BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	224,846	T→C	noncoding (1076/1542 nt)	rrsH →	16S ribosomal RNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	224,846	0	T	C	86.4%	15.3 / 67.0	242	noncoding (1076/1542 nt)	rrsH	16S ribosomal RNA
Reads supporting (aligned to +/- strand): ref base T (19/13); new base C (110/93); total (133/109)
Fisher's exact test for biased strand distribution p-value = 7.03e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 255 total aligned reads displayed.

GCGGTGGAGCATGTGGTTTAATTCGATGCAACGCGAAGAACCTTACCTGGTCTTGACATCCACAGAACTTTCCAGAGATGGATTGGTGCCTTCGGGAACTGTGAGACAGGTGCTGCATGGCTGTCGTCAGCTCGTGTTGTGAAATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATCTTTTGTTGCCAGC‑GGTCCGGCCGGGAACTCAAAGGAGACTGCCAGTGATAAACTGGAGGAAGGTGGGGATGACGTCAAGTCATCATGGCCCTT  >  NC_000913/224709‑224982

           tgtgGTTTAATTCGATGCAACGCGAAGAACCTTACCTGGTCTTGACATCCACAGAACTTTCCAGAGATGGATTGGTGCCTTCGGGAACTGTGAGACAGGTGCTGCATGGCTGTCGTCAGCTCGTGTTGTGAAATGTTggg                                                                                                                              >  2:1803412/1‑140 (MQ=255)

             tgGTTTAATTCGATGCAACGCGAAGAACCTTACCTGGTCTTGACATCCACAGAACTTTCCAGAGATGGATTGGTGCCTTCGGGAACTGTGAGACAGGTGCTGCATGGCTGTCGTCAGCTCGTGTTGTGAAATGTTGGGtt                                                                                                                            <  1:1909567/140‑1 (MQ=255)

                     ttCGATGCAACGCGAAGAACCTTACCTGGTCTTGACATCCACAGAACTTTCCAGAGATGGATTGGTGCCTTCGGGAACTGTGAGACAGGTGCTGCATGGCTGTCGTCAGCTCGTGTTGTGAAATGTTGGGTTAAGTCCCg                                                                                                                    >  1:1744867/1‑140 (MQ=255)

                         aTGCAACGCGAAGAACCTTACCTGGTCTTGACATCCACAGAACTTTCCAGAGATGGATTGGAGCCTTCGGGAACTGTGAGACAGGTGCTGCATGGCTGTCGTCAGCTCGTGTTGTGAAATGTTGGGTTAAGTCCCGCAAc                                                                                                                >  1:1611368/1‑140 (MQ=255)

                            cAACGCGAAGAACCTTACCTGGTCTTGACATCCACAGAACTTTCCAGAGATGGATTGGTGCCTTCGGGAACTGTGAGACAGGTGCTGCATGGCTGTCGTCAGCTCGTGTTGTGAAATGTTGGGTTAAGTCCCGCAACGAg                                                                                                             <  2:1912711/140‑1 (MQ=255)

                                       aCCTTACCTGGTCTTGACATCCACAGAACTTTCCAGAGATGGATTGGTGCCTTCGGGAACTGTGAGACAGGTGCTGCATGGCTGTCGTCAGCTCGTGTTGTGAAATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTa                                                                                                  >  1:1831563/1‑140 (MQ=255)

                                                                                        ccTTCGGGAACTGTGAGACAGGTGCTGCATGGCTGTCGTCAGCTCGTGTTGTGAAATGTTGGGTTAAGTCCCGCAACGAGCGGAACCCTTATCTTTTGTTGCCAGC‑GGTCCGGCCGGGAACTCAAAGGAGACTGCCAGTg                                                <  2:1611368/140‑1 (MQ=21)

                                                                                                  attcaagaCAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCgga                                      <  2:1518746/135‑1 (MQ=2)

                                                                                                  attaaagaCAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCgga                                      <  2:595557/135‑1 (MQ=2)

                                                                                                   ttcaagaCAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCggag                                     >  1:573227/5‑140 (MQ=2)

                                                                                                   ttcaagaCAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCggag                                     >  1:243978/5‑140 (MQ=2)

                                                                                                   tgtgAGTCAGGTGCTGCATGGCTGTCGTCAGCTCGTGTTGTGAAATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATCTTTTGTTGCCAGC‑GGTCCGGCCGGGAACTCAAAGGAGACTGCCAGTGATAAACTggag                                     <  2:2116611/140‑1 (MQ=18)

                                                                                                    tcaataCAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGCACGTAAGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCggag                                     >  1:1331644/6‑140 (MQ=2)

                                                                                                    tcaagaCAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCggagg                                    >  1:1273717/4‑140 (MQ=2)

                                                                                                     caagaCAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCggagga                                   <  2:2022922/138‑1 (MQ=2)

                                                                                                        taCAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTTGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAgg                                >  2:266413/2‑140 (MQ=2)

                                                                                                        gaCAGGTG‑TGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTTGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGt                               <  2:425261/140‑1 (MQ=2)

                                                                                                         aCAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGt                               <  1:755505/140‑1 (MQ=2)

                                                                                                          cAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCTCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTg                              <  2:1428604/140‑1 (MQ=2)

                                                                                                          cAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCACA‑CGTAAGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTg                              <  2:444149/140‑1 (MQ=2)

                                                                                                           aGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCggagg                                    <  1:747085/133‑1 (MQ=2)

                                                                                                            ggttgtgcATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTggg                            >  2:652170/6‑140 (MQ=2)

                                                                                                            ggtggtGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTggg                            >  1:963931/1‑140 (MQ=2)

                                                                                                            ggtggtGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTggg                            >  1:1544929/1‑140 (MQ=2)

                                                                                                             gtggtgcATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTTGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAgg                                >  2:1228859/5‑135 (MQ=2)

                                                                                                             gtggtgcATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTTGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTgggg                           >  1:1509181/5‑140 (MQ=2)

                                                                                                             gtggtgcATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTgggg                           >  2:887805/5‑140 (MQ=2)

                                                                                                             gtggtgcATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAAc                                          >  2:1210536/5‑125 (MQ=2)

                                                                                                             gtggtgcATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTgggg                           >  1:1443907/5‑140 (MQ=2)

                                                                                                             gtggtgcATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTgggg                           >  1:539176/5‑140 (MQ=2)

                                                                                                             gtggtgCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTTGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTgggg                           >  2:1530831/1‑140 (MQ=2)

                                                                                                             gtggtgCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTTGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTgggg                           >  1:2293851/1‑140 (MQ=2)

                                                                                                             gtggtgCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTTGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTgggg                           >  2:1315409/1‑140 (MQ=2)

                                                                                                             gtggtgCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGTGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTgggg                           >  2:1183226/1‑140 (MQ=2)

                                                                                                             gtggtgCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTgggg                           >  1:446246/1‑140 (MQ=2)

                                                                                                             gtggtgCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTgggg                           >  2:928143/1‑140 (MQ=2)

                                                                                                             gtggtgCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTgggg                           <  1:2279759/140‑1 (MQ=2)

                                                                                                             gtggtgCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAAAGAGACTGCCGGTGACaaa                                           <  2:1191616/124‑1 (MQ=2)

                                                                                                             gtggtgCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAAAGAGACTGCCGGTGACaaa                                           >  1:1191616/1‑124 (MQ=2)

                                                                                                             gtggtgCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTgggg                           >  2:584196/1‑140 (MQ=2)

                                                                                                             gtggtgCATGGTTGTAGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCggag                                     >  1:380151/1‑130 (MQ=2)

                                                                                                             gTGCTGCATGGCTGTCGTCAGCTCGTGTTGTGAAATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATCTTTTGTTGCCAGC‑GGTCCGGCCGGGAACTCAAAGGAGACTGCCAGTGATAAACTGGAGGAAGGTgggg                           >  1:1797692/1‑140 (MQ=32)

                                                                                                             gTGCTGCATGGCTGTCGTCAGCTCGTGTTGTGAAATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATCTTTTGTTGCCAGC‑GGTCCGGCCGGGAACTCAAAGGAGACTGCCAGTGATAAACTGGAGGAAGGTgggg                           <  2:2193877/140‑1 (MQ=32)

                                                                                                              tggtgcATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGa                          >  1:451761/4‑140 (MQ=2)

                                                                                                               ggtgcATGGTTGTCGTCAGCTCGTGTCGTTAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGAt                         <  1:1220722/138‑1 (MQ=2)

                                                                                                               ggtgcATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGAt                         <  2:782366/138‑1 (MQ=2)

                                                                                                                gtgcATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGTGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATg                        >  2:1001251/2‑140 (MQ=2)

                                                                                                                gtgcATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTg                              <  2:166158/133‑1 (MQ=2)

                                                                                                                  gcATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGAc                      >  2:673291/1‑140 (MQ=11)

                                                                                                                   cATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGCACGTAAGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGAc                      <  1:206258/140‑1 (MQ=2)

                                                                                                                   cATGGCTGTCGTCAGCTCGTGTTGTGAAATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATCTTTTGTTGCCAGC‑GGTCCGGCCGGGAACTCAAAGGAGACTGCCAGTGATAAACTGGAGGAAGGTGGGGATGACg                     >  2:2162061/1‑140 (MQ=32)

                                                                                                                   cATGGCTGTCGTCAGCTCGTGTTGTGAAATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATCTTTTGTTGCCAGC‑GGTCCGGCCGGGAACTCAAAGGAGACTGCCAGTGATAAACTGGAGGAAGGTGGGGATGACg                     >  1:1676752/1‑140 (MQ=32)

                                                                                                                     tGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTTGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCggagg                                    >  1:337969/1‑123 (MQ=2)

                                                                                                                      ggttGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCa                  <  1:1452330/137‑1 (MQ=2)

                                                                                                                      ggttGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTCAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCa                  <  2:71082/137‑1 (MQ=2)

                                                                                                                       gttGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGCACGTAAGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCa                  >  2:1039926/3‑140 (MQ=2)

                                                                                                                       gttGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTTGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTg                              <  2:537893/125‑1 (MQ=2)

                                                                                                                       gttGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTTGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCaa                 <  1:215079/138‑1 (MQ=2)

                                                                                                                       gttGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGAt                         >  2:378154/3‑132 (MQ=2)

                                                                                                                       gttGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCaa                 >  2:232811/3‑140 (MQ=2)

                                                                                                                       gttGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCaa                 >  1:1800566/3‑140 (MQ=11)

                                                                                                                       gttGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCaa                 >  2:1417433/3‑140 (MQ=11)

                                                                                                                        ttGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGAt                         <  1:1066580/130‑1 (MQ=2)

                                                                                                                        ttGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAATTGCCAGC‑ACTTCGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCaaa                <  2:1485163/139‑2 (MQ=2)

                                                                                                                         tGTCGTCAGCTCGTGTTGTGAAATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATCTTTTGTTGCCAGC‑GGTCCGGCCGGGAACTCAAAGGAGACTGCCAGTGATAAACTGGAGGAAGGTGGGGATGACGTCAAGt               <  2:1831563/140‑1 (MQ=32)

                                                                                                                         tGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTTGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCaaat               <  1:901263/140‑3 (MQ=2)

                                                                                                                          gtcgtcAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGCACGTAAGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCaaat               <  1:1039926/140‑3 (MQ=2)

                                                                                                                           tcgtcAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGCACGTAAGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAAtc              >  2:1064/1‑140 (MQ=2)

                                                                                                                           tcgtcAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTTGGGTGGACACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAAtca             >  2:1460881/1‑140 (MQ=2)

                                                                                                                           tcgtcAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAAtca             >  2:397489/1‑140 (MQ=2)

                                                                                                                           tcgtcAGCTCGTGTAGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTTGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAAtca             >  1:99624/1‑140 (MQ=2)

                                                                                                                           tcgtcAGCTCGTGTAGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTTGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAAtca             >  1:649172/1‑140 (MQ=2)

                                                                                                                            cgtcAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAAtcat            <  1:673291/140‑1 (MQ=2)

                                                                                                                                aGCTCGTGTCGTTAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATg        <  1:583765/140‑1 (MQ=2)

                                                                                                                                aGCTCGTGTCGTTAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATg        <  1:772630/140‑1 (MQ=2)

                                                                                                                                aGCTCGTGTCGTGAGATGTTTGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAatcatc           <  2:1092613/137‑1 (MQ=2)

                                                                                                                                aGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTTGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATg        >  2:299278/1‑140 (MQ=2)

                                                                                                                                aGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTTGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATg        <  2:2273377/140‑1 (MQ=2)

                                                                                                                                aGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTTGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATg        <  1:113453/140‑1 (MQ=2)

                                                                                                                                aGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGTGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATg        <  1:1001251/140‑1 (MQ=2)

                                                                                                                                aGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATAATg        <  1:1251541/140‑1 (MQ=2)

                                                                                                                                 gCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACTAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATgc       <  1:304893/140‑2 (MQ=2)

                                                                                                                                 gCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGCACGTAAGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGt                    >  2:489725/1‑128 (MQ=2)

                                                                                                                                 gCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGCACGTAAGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATg        <  2:326425/140‑1 (MQ=2)

                                                                                                                                 gCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTTGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATgc       <  1:153237/140‑2 (MQ=2)

                                                                                                                                 gCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATgc       <  2:963931/140‑2 (MQ=2)

                                                                                                                                 gCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATgc       <  2:474466/140‑2 (MQ=2)

                                                                                                                                 gCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATgc       <  1:397489/140‑2 (MQ=2)

                                                                                                                                 gCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATgc       <  2:1345317/140‑2 (MQ=2)

                                                                                                                                 gCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATgc       <  2:556350/140‑2 (MQ=2)

                                                                                                                                 gCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATgc       <  2:1305825/140‑2 (MQ=2)

                                                                                                                                 gCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCCAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATgc       <  2:688431/140‑2 (MQ=2)

                                                                                                                                  cTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATgcc      >  1:1368468/1‑138 (MQ=2)

                                                                                                                                  cTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATgcc      >  1:494582/1‑138 (MQ=2)

                                                                                                                                   tcgtgtcgtgAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATgccc     <  1:1421999/140‑4 (MQ=2)

                                                                                                                                     gtgtCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGTAAGGTGGGGATGACGTCAAATCATCATGCCCCt   >  1:1204009/1‑140 (MQ=2)

                                                                                                                                      tgtcgtGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTTGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCtt  <  1:92105/140‑1 (MQ=2)

                                                                                                                                      tgtcgtGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCtt  <  2:836452/140‑1 (MQ=2)

                                                                                                                                      tgtcgtGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCtt  <  1:876197/140‑1 (MQ=2)

                                                                                                                                      tgtagtGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCtt  <  2:830041/140‑1 (MQ=2)

GCGGTGGAGCATGTGGTTTAATTCGATGCAACGCGAAGAACCTTACCTGGTCTTGACATCCACAGAACTTTCCAGAGATGGATTGGTGCCTTCGGGAACTGTGAGACAGGTGCTGCATGGCTGTCGTCAGCTCGTGTTGTGAAATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATCTTTTGTTGCCAGC‑GGTCCGGCCGGGAACTCAAAGGAGACTGCCAGTGATAAACTGGAGGAAGGTGGGGATGACGTCAAGTCATCATGGCCCTT  >  NC_000913/224709‑224982

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`