BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	224,933	A→G	noncoding (1163/1542 nt)	rrsH →	16S ribosomal RNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	224,933	0	A	G	91.3%	112.5 / 75.8	437	noncoding (1163/1542 nt)	rrsH	16S ribosomal RNA
Reads supporting (aligned to +/- strand): ref base A (19/19); new base G (173/224); total (193/244)
Fisher's exact test for biased strand distribution p-value = 4.96e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.65e-01

Only 100 of 437 total aligned reads displayed.

CCTTCGGGAACTGTGAGACAGGTGCTGCATGGCTGTCGTCAGCTCGTGTTGTGAAATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATCTTTTGTTGCCAGC‑GGTCCGGCCGGGAACTCAAAGGAGACTGCCAGTGATAAACTGGAGGAAGGTGGGGATGACGTCAAGTCATCATGGCCCTTACGACCAGGGCTACACACGTGCTACAATGGCGCATACAAAGAGAAG  >  NC_000913/224797‑225028

ccTTCGGGAACTGTGAGACAGGTGCTGCATGGCTGTCGTCAGCTCGTGTTGTGAAATGTTGGGTTAAGTCCCGCAACGAGCGGAACCCTTATCTTTTGTTGCCAGC‑GGTCCGGCCGGGAACTCAAAGGAGACTGCCAGTg                                                                                              <  2:1611368/140‑1 (MQ=21)

  ttCGGGAACTGTGAGACAGGTGCTGCATGGCTGTCGTCAGCTCGTGTTGTGAAATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATCTTTTGTTGCCAGC‑GGTCCGGCCGGGAACTCAAAGGAGACTGCCAGTGAt                                                                                            >  1:1897735/1‑140 (MQ=34)

          cTGTGAGACAGGTGCTGCATGGCTGTCGTCAGCTCGTGTTGTGAAATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATCTTTTGTTGCCAGC‑GGTCCGGCCGGGAACTCAAAGGGGACTGCCAGTGATAAACTgga                                                                                    >  1:1771882/1‑140 (MQ=18)

          attcaagaCAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCgga                                                                                    <  2:1518746/135‑1 (MQ=2)

           ttcaagaCAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCggag                                                                                   >  1:243978/5‑140 (MQ=2)

           tgtgAGTCAGGTGCTGCATGGCTGTCGTCAGCTCGTGTTGTGAAATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATCTTTTGTTGCCAGC‑GGTCCGGCCGGGAACTCAAAGGAGACTGCCAGTGATAAACTggag                                                                                   <  2:2116611/140‑1 (MQ=18)

            tcaagaCAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCtgagg                                                                                  <  1:1447468/137‑1 (MQ=2)

            tcaagaCAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCggagg                                                                                  >  1:1273717/4‑140 (MQ=2)

             caagaCAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCggagga                                                                                 <  2:1894016/138‑1 (MQ=2)

               agaCAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTTGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAg                                                                               >  2:1042822/1‑140 (MQ=2)

                gaCAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAgg                                                                              <  2:831425/140‑1 (MQ=2)

                 aCAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTTAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGt                                                                             >  2:1220722/1‑140 (MQ=2)

                  cAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCTCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTg                                                                            <  2:1428604/140‑1 (MQ=2)

                   aGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCggagg                                                                                  <  1:747085/133‑1 (MQ=2)

                    ggttgtgcATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTggg                                                                          >  2:652170/6‑140 (MQ=2)

                    ggtggtGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTggg                                                                          >  1:1544929/1‑140 (MQ=2)

                    ggtggtGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTggg                                                                          >  1:1253238/1‑140 (MQ=2)

                     gtggtgCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGCACGTAAGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTggg                                                                          >  2:1539596/1‑140 (MQ=2)

                     gtggtgCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGCACGTAAGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTggg                                                                          >  1:326425/1‑140 (MQ=2)

                     gtggtgCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTTGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTgggg                                                                         >  2:1315409/1‑140 (MQ=2)

                     gtggtgCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTTGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTgggg                                                                         >  1:2293851/1‑140 (MQ=2)

                     gTGCTGCATGGCTGTCGTCAGCTCGTGTTGTGAAATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATCTTTTGTTGCCAGC‑GGTCCGGCCGGGAACTCAAAGGAGACTGCCAGTGATAAACTGGAGGAAGGTgggg                                                                         >  2:1706359/1‑140 (MQ=32)

                      tggtgcATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCAGCAACGAGCGCAACCCTTATTTTTAGTTGCCAGC‑ACTTTGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGa                                                                        >  1:1421928/4‑140 (MQ=2)

                       ggtgcATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGAt                                                                       <  2:451761/138‑1 (MQ=2)

                          gcATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGAc                                                                    <  2:706486/140‑1 (MQ=2)

                          gcATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGAc                                                                    >  2:673291/1‑140 (MQ=11)

                           cATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACg                                                                   <  2:1026543/140‑1 (MQ=2)

                               gttGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTTGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCaa                                                               <  1:215079/138‑1 (MQ=2)

                               gttGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCaa                                                               <  2:616548/138‑1 (MQ=2)

                               gttGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAGAAAGACTGCAGGTGACAAACCGGAGGAAGGTGGGGATGACGTCaa                                                               >  1:1312796/3‑140 (MQ=2)

                               gttGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCaa                                                               >  1:1800566/3‑140 (MQ=11)

                               gttGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCATATTTTTAGTTGCCAGC‑ACTTTGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACg                                                                   >  1:425964/3‑136 (MQ=2)

                                ttGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGCACGTAAGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCaa                                                               >  2:929554/2‑140 (MQ=2)

                                ttGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGAt                                                                       <  1:1066580/130‑1 (MQ=2)

                                ttGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCaaa                                                              >  2:237002/2‑139 (MQ=2)

                                ttGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAATTGCCAGC‑ACTTCGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCaaa                                                              <  2:1485163/139‑2 (MQ=2)

                                 tGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGCACGTAAGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCaaa                                                              >  2:1192789/1‑139 (MQ=2)

                                   tcgtcAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGCACGTAAGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAAtc                                                            >  2:1064/1‑140 (MQ=2)

                                   tcgtcAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAGAAAGACTTCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAAtca                                                           >  1:572126/1‑140 (MQ=2)

                                   tcgtcAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAAtca                                                           >  2:78836/1‑140 (MQ=11)

                                   tcgtcAGCTCGTGTAGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTTGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAAtca                                                           >  1:649172/1‑140 (MQ=2)

                                   tcgtcAGCTCGTGTAGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTTGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAAtca                                                           >  1:99624/1‑140 (MQ=2)

                                    cgtcAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAAtcat                                                          <  1:673291/140‑1 (MQ=2)

                                     gtcAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGCACGTAAGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAAtcat                                                          >  1:1210183/1‑140 (MQ=2)

                                        aGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGTGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATg                                                      <  1:1001251/140‑1 (MQ=2)

                                         gCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGCACGTAAGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGt                                                                  >  2:489725/1‑128 (MQ=2)

                                         gCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGCACGTAAGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATg                                                      <  2:326425/140‑1 (MQ=2)

                                         gCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTTGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATgc                                                     <  2:1820196/140‑2 (MQ=2)

                                         gCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTTGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATgc                                                     <  2:411908/140‑2 (MQ=2)

                                         gCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTTGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATgc                                                     <  1:1530831/140‑2 (MQ=2)

                                         gCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATgc                                                     <  1:397489/140‑2 (MQ=2)

                                         gCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATgc                                                     >  2:2192665/1‑139 (MQ=2)

                                          cTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTTGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATgcc                                                    >  1:104563/1‑138 (MQ=2)

                                          cTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTTGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATgcc                                                    >  1:824503/1‑138 (MQ=2)

                                          cTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATgcc                                                    >  1:899819/1‑138 (MQ=2)

                                          cTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATgcc                                                    >  2:576799/1‑138 (MQ=2)

                                          cTCGTGTAGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGCACGTAAGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACCTCAAATCATCATgc                                                     >  1:456263/1‑139 (MQ=2)

                                              tgtcgtGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTTGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCtt                                                <  1:913327/140‑1 (MQ=2)

                                              tgtcgtGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCtt                                                <  2:549385/140‑1 (MQ=2)

                                               gtcgtgAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATgc                                                     <  2:53369/131‑2 (MQ=2)

                                               gtcgtgAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATgc                                                     >  1:53369/4‑133 (MQ=2)

                                               gtcgtgAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTa                                               <  2:966293/137‑1 (MQ=2)

                                                tcgtgAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCATATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTat                                              <  1:378169/138‑2 (MQ=2)

                                                 cgtgAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTatg                                             <  1:984957/139‑3 (MQ=2)

                                                  gtgAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGCACGTAAGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTatg                                             >  1:117973/1‑138 (MQ=2)

                                                   tgAAATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATCTTTTGTTGCCAGC‑GGTCCGGCCGGGAACTCAAAGGAGACTGCCAGTGATAAACTGGAGGAAGGTGGGGATGACGTCAAGTCATCATGGCCCTTACGAc                                           <  1:1620517/140‑1 (MQ=32)

                                                    gagaTGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGCACGTAAGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGAc                                           <  2:431246/137‑1 (MQ=2)

                                                    gagaTGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGAcc                                          <  2:1134330/137‑1 (MQ=2)

                                                    gagaTGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGAcc                                          >  2:392565/4‑140 (MQ=2)

                                                    gagaTGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGAcc                                          <  2:1411019/137‑1 (MQ=2)

                                                         gTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTAT‑TTTAGTTGCCAGC‑ACTTTGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGCt                                    >  2:585829/1‑140 (MQ=2)

                                                            gggTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAAAGAGACTGCCGGTTACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGCTac                                  <  2:373962/140‑1 (MQ=2)

                                                            gggTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATAATGCCCCTTATGACCTGGGCTac                                  <  1:497815/140‑1 (MQ=2)

                                                             ggTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGTCAGCACGTAAGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGCTac                                  <  1:2216155/140‑1 (MQ=2)

                                                             ggTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGCACGTAAGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGCTac                                  >  1:943343/1‑140 (MQ=2)

                                                             ggTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTTGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGCt                                    >  1:1347507/1‑137 (MQ=2)

                                                             ggTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTTGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGCt                                    <  2:1347507/137‑1 (MQ=2)

                                                             ggTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATAATGCCCCTTATGACCTGGGCTaca                                 >  2:1265682/1‑140 (MQ=2)

                                                             ggTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAGAAAGACTACCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGAcc                                          >  2:1178933/1‑131 (MQ=2)

                                                              gTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTTGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGCTacac                                <  1:1232537/140‑1 (MQ=2)

                                                              gTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGCTacac                                <  1:115504/140‑1 (MQ=2)

                                                              gTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGCTacac                                <  1:585029/140‑1 (MQ=2)

                                                               ttAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGCACGTAAGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGCTacac                                <  2:748102/140‑1 (MQ=2)

                                                               ttAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATAATCATGCCCCTTATGAc                                           >  2:130353/1‑128 (MQ=2)

                                                                tAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGCACGTAAGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGCTacaca                               <  1:147754/140‑1 (MQ=2)

                                                                   gTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTTGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGCTACACACGta                           <  1:771847/140‑2 (MQ=2)

                                                                   gTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGCTACACACGta                           <  2:895913/140‑2 (MQ=2)

                                                                       cGCAACGAGCGCAACCCTTATCTTTTGTTGCCAGC‑GGTCCGGCCGGGAACTCAAAGGAGACTGCCAGTGATAAACTGGAGGAAGGTGGGGATGACGTCAAGTCATCATGGCCCTTACGACCAGGGCTACACACGTGCTAc                       >  2:2282579/1‑140 (MQ=33)

                                                                       cGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCATTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGCTa                                   >  2:1454463/1‑128 (MQ=2)

                                                                       cGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGCTacac                                >  1:1452463/1‑131 (MQ=2)

                                                                       cGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATAATCATGCCCCTTATGACCTGGGc                                     >  2:874805/1‑126 (MQ=2)

                                                                       cGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATAATCATGCCCCTTATGACCTGGGc                                     <  1:874805/126‑1 (MQ=2)

                                                                        gCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTTGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGCTACACACGTACTACa                      <  1:2059846/140‑1 (MQ=2)

                                                                        gCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTTGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGCTACACACGTACTACa                      <  1:1685945/140‑1 (MQ=2)

                                                                            cGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGTGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGCTACACACGTACTACAATg                   >  1:346849/1‑139 (MQ=2)

                                                                             gAGCGCAACCCTTATCTTTTGTTGCCAGC‑GGTCCGGCCGGGAACTCAAAGGAGACTGCCAGTGATAAACTGGAGGAAGGTGGGGATGACGTCAAGTCATCATGGCCCTTACGACCAGGGCTACACACGTGCTACAATGgc                 <  2:2168815/140‑1 (MQ=32)

                                                                              aGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTTGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGCTACACACGTACTACAATGgcc                >  2:2008989/1‑139 (MQ=2)

                                                                              aGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTTGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGCTACACACGTACTACAATGgcc                >  1:2091896/1‑139 (MQ=2)

                                                                              aGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTTGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGCTACACACGTACTACAATGgcc                >  2:298246/1‑139 (MQ=2)

                                                                              aGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTTGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGCTACACACGTACTACAATGgcc                >  2:1102089/1‑139 (MQ=2)

CCTTCGGGAACTGTGAGACAGGTGCTGCATGGCTGTCGTCAGCTCGTGTTGTGAAATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATCTTTTGTTGCCAGC‑GGTCCGGCCGGGAACTCAAAGGAGACTGCCAGTGATAAACTGGAGGAAGGTGGGGATGACGTCAAGTCATCATGGCCCTTACGACCAGGGCTACACACGTGCTACAATGGCGCATACAAAGAGAAG  >  NC_000913/224797‑225028

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`