Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	225,531	T→C	noncoding (32/76 nt)	alaV →	tRNA‑Ala

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	225,531	0	T	C	78.2%	195.5 / 44.0	88	noncoding (32/76 nt)	alaV	tRNA‑Ala
Reads supporting (aligned to +/- strand):  ref base T (10/9);  new base C (33/35);  total (44/44)
Fisher's exact test for biased strand distribution p-value = 8.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.16e-01

GTGGTTAGAGCGCACCCCTGATAAGGGTGAGGTCGGTGGTTCAAGTCCACTCAGGCCTACCAAATTTGCACGGCAAATTTGAAGAGGTTTTAACTACATGTTATGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCACCATCTCTGTAGTGGTTAAATAAAAAATACTTCAGAGTGTACCTGCAAAGGTTCACTGCGAAGTTTTGCTCTTTAAAAATCTGGATCAAGCTGAAAA  >  NC_000913/225396‑225669                                                                                                                                        |                                                                                                                                           cctttagctcagctggttagagcgcacccctgataagggtgaggtcggtggttcgagtccactaaggcccaccattatcaacacatgagtgttgatcccaTTAAGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGc                                                                                                                                        <  1:1900064‑M1/40‑1 (MQ=255)  ctttagctcagctggttagagagcacccctgataagggtgaggtcggtggttcgagtccactaaggcccaccattatcaacacatgagtgttgatcccaTTAAGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGCa                                                                                                                                       >  2:373424‑M1/100‑140 (MQ=255)   tttagctcagctggttagagcgcacccctgataagggtgaggtcggtggttcgagtccactaaggcccaccattatcaacacatgagtgttgatcccaTTAAGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGcaa                                                                                                                                      <  2:1346434‑M1/42‑2 (MQ=255)    ttagctcagctggttagagcgcacccctgataagggtgaggtcggttgttcgagtccactaaggcccaccattatcaacacatgagtgttgatcccaatacGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTgcaag                                                                                                                                     >  2:1436364‑M1/102‑138 (MQ=255)    ttagctcagctggttagagcgcacccctgataagggtgaggtcggtggttcgagtccactaaggcccgccattatcaacacatgagtgttgatcccaTTAAGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTgcaag                                                                                                                                     <  2:2169636‑M1/43‑3 (MQ=255)    ttagctcagctggttagagcgcacccctgataagggtgaggtcggtggttcgagtccactaaggcccaccattatcaacacatgagtgttgatcccaTTAAGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTgcaag                                                                                                                                     >  2:1121051‑M1/98‑138 (MQ=255)       gctcagctggttagagcgcacccctgataagggtgaggtcggtggttcgagtccactaaggcccaccattatcaacacatgagtgttgatcccaatacGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGCAAGCag                                                                                                                                  >  2:249496‑M1/99‑140 (MQ=255)         tcagctggttagagcgcacccctgataagggtgaggtcggtggttctagtccactaaggcccaccattatcaacacatgagtgttgatcccaatacGGGGCCATAGCTCAGCTGCGAGAGCGCCTGCCTTGCAAGCagga                                                                                                                                >  1:1411760‑M1/97‑140 (MQ=255)         tcagctggttagagcgcacccctgataagggtgaggtcggtggttcgagtccactaaggcccaccattatcaacacatgagtgttgatcccaatacGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGCAAGCagga                                                                                                                                >  2:19965‑M1/97‑140 (MQ=255)          cagctggttagagcgcacccctgataagggtgaggtcggtggttcgagtccactaaggcccaccattatcaacacatgagtgttgatcccaatacGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGCAAGCaggag                                                                                                                               <  1:1294063‑M1/45‑1 (MQ=255)           agctggttagagcgcacccctgataagggtgaggtcggtggttcgagtccactaaggcccaccattatcaacacatgagtgttgatcccaTTAAGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGCAAGCaggagg                                                                                                                              <  2:1384288‑M1/50‑1 (MQ=255)             ctggttagagcgcacccctgataagggtgaggtcggtggttcgagtccactaaggcccaccattatcaacacatgagtgttgatcccaatacGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGCAAGCAGGAGGTc                                                                                                                            >  1:1451511‑M1/93‑140 (MQ=255)              tggttagagcgcacccctgataagggtgaggtcggtggttcgagtccactaaggcccaccattatcaacacatgagtgttgatcccaatacGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGCAAGCAGGAGGtca                                                                                                                           >  2:2098445‑M1/92‑139 (MQ=255)               ggttagagcgcacccctgataagggtgaggtcggtggttcgagtccactaaggcccaccattatcaacacatgagtgttgatcccaatacGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGCAAGCAGGAGgtcag                                                                                                                          >  1:726719‑M1/91‑138 (MQ=255)               ggttagagcgcacccctgataagggtgaggtcggtggttcgagtccactaaggcccaccattatcaacacatgagtgttgatcccaatacGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGCAAGCAGGAGgtcag                                                                                                                          >  1:815255‑M1/91‑138 (MQ=255)               ggttagagcgcacccctgataagggtgaggtcggtggttcgagtccactaaggcccaccattatcaacacatgagtgttgatcccaatacGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGCAAGCAGGAGgtcag                                                                                                                          >  1:255996‑M1/91‑138 (MQ=255)               ggttagagcgcacccctgataagggtgaggtcggtggttcgagtccactaaggcccaccattatcaacacatgagtgttgatcccaatacGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGCAAGCAGGAGgtcag                                                                                                                          >  2:55879‑M1/91‑138 (MQ=255)               ggttagagcgcacccctgataagggtgaggtcggtggttcgagtccactaaggcccaccattatcaacacatgagtgttgatcccaTTAAGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGCAAGCAGGAGgtcag                                                                                                                          >  1:1372127‑M1/87‑138 (MQ=255)                     agcgcacccctgataagggtgaggtcggtggttcgagtccactaaggcccaccattatcaacacatgagtgttgatcccaTTAAGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGCAAGCAGGAGGTCAGCGGTTc                                                                                                                    <  1:1376237‑M1/60‑1 (MQ=255)                       cgcacccctgataagggtgaggtcggtggttcgagtccactaaggcccaccattatcaacacatgagtgtttatcccaatacGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGCAAGCAGGAGGTCAGCGGTTCGa                                                                                                                  <  2:191391‑M1/58‑1 (MQ=255)                       cgcacccctgataagggtgaggtcggtggttcgagtccactaaggcccaccattatcaacacatgagtgttgatcccaatacGGGGCCATAGCTCAGCTGGGATAGCGCCTGCCTTGCAAGCAGGAGGTCAGcggttaga                                                                                                                  <  2:1186710‑M1/58‑4 (MQ=255)                       cgcacccctgataagggtgaggtcggtggttcgagtccactaaggcccaccattatcaacacatgagtgttgatcccaatacGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGCAAGCAGGAGGTCAGCGGTTCGa                                                                                                                  <  1:213827‑M1/58‑1 (MQ=255)                       cgcacccatgataagggtgaggtcggtggttcgagtccactaaggcccaccattatcaacacatgagtgttgatcccaTTAAGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGCAAGCAGGAGGTCAGCGGTTCGa                                                                                                                  <  1:881194‑M1/62‑1 (MQ=255)                        gcacccctgataagggtgaggtcggtggttcgagtccactaaggcccaccattatcaacacatgagtgttgatcccaTTAAGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGCAAGCAGGAGGTCAGCGGTTCGAt                                                                                                                 >  2:435597‑M1/78‑140 (MQ=255)                         cacccctgataagggtgaggtcggtggttcgagtccactaaggcccaccattatcaacacatgagtgttgatcccaatacGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGCAAGCAGGAGGTCAGCGGTTCGATc                                                                                                                >  2:1294063‑M1/81‑140 (MQ=255)                         cacccctgataagggtgaggtcggtggttcgagtccactaaggcccaccattatcaacacatgagtgttgatcccaatacGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGCAAGCAGGAGGTCAGCGGTTCGATc                                                                                                                >  1:384781‑M1/81‑140 (MQ=255)                          acccctgataagggtgaggtcggtggttcgagtccactaaggcccaccattatcaacacatgagtgttgatcccaatacGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGCAAGCAGGAGgtcag                                                                                                                          <  2:1171190‑M1/50‑3 (MQ=255)                          acccctgataagggtgaggtcggtggttcgagtccactaaggcccaccattatcaacacatgagtgttgatcccaatacGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGCAAGCAGGAGgtcag                                                                                                                          >  1:1171190‑M1/80‑127 (MQ=255)                            ccctgataagggtgaggtcggtggttcgagtccactaaggcccaccattatcaacacatgagtgttgatcccaatacGGGGCCATAGCTCAGCTGGGATAGCGCCTGCCTTGCAAGCAGGAGGTCAGCGGTTAGATCCCg                                                                                                             >  1:1186710‑M1/78‑140 (MQ=255)                            ccctgataagggtgaggtcggtggttcgagtccactaaggcccaccattatcaacacatgagtgttgatcccaatacGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGCAAGCAGGAGGTCAGCGGTTCGATCCCg                                                                                                             <  1:983467‑M1/63‑1 (MQ=255)                            ccctgataagggtgaggtaggtggttcgagtccactaaggcccaccattatcaacacatgagtgttgatcccaatacGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGCAAGCAGGAGGTCAGCGGTTCGATCCCg                                                                                                             <  1:531534‑M1/63‑1 (MQ=255)                             cctgataagggtgaggtcggtggttcgagtccactaaggcccaccattatcaacacatgagtgttgatcccaatacGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGCAAGCAGGAGGTCAGCGGTTCGATCCCGc                                                                                                            >  2:983467‑M1/77‑140 (MQ=255)                             cctgataagggtgaggtcggtggttcgagtccactaaggcccaccattatcaacacatgagtgttgatcccaTTAAGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCATTGCAAGCAGGAGGTCAGCGGTTCGATCCCGc                                                                                                            >  1:673176‑M1/73‑140 (MQ=255)                               tgataagggtgaggtcggtggttcgagtccactaaggcccaccattatcaacacatgagtgttgatcccaatacGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGCAAGCAGGAGGTCAGTGGTTCGATCCcgctt                                                                                                          >  1:888155‑M1/75‑138 (MQ=255)                               tgataagggtgaggtcggtggttcgagtccactaaggcccaccattatcaacacatgagtgttgatcccaatacGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGCAAGCAGGAGGTCAGCGGTTCGATCCcgctt                                                                                                          >  2:104934‑M1/75‑138 (MQ=255)                               tgataagggtgaggtcggtggttcgagtccactaaggcccaccattatcaacacatgagtgttgatcccaatacGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGCAAGCAGGAGGTCAGCGGTTCGATCCcgctt                                                                                                          >  1:235358‑M1/75‑138 (MQ=255)                               tgataagggtgaggtcggtggttcgagtccactaaggcccaccattatcaacacatgagtgttgatcccaatacGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGCAAGCAGGAGGTAAGCGGTTCGATCCcgctt                                                                                                          >  2:704249‑M1/75‑138 (MQ=255)                                 ataagggtgaggtcggtggttcgagtccactaaggcccaccattatcaacacatgagtgttgatcccaatacGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGCAAGCAGGAGGTCAGCGGTTCGATcccgcttgg                                                                                                        <  2:77029‑M1/68‑5 (MQ=255)                                 ataagggtgaggtcggtggttcgagtccactaaggcccaccattatcaacacatgagtgttgatcccaTTAAGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGCAAGCAGGAGGTCAGCGGTTCGATcccgcttgg                                                                                                        <  1:540404‑M1/72‑5 (MQ=255)                                  taatggtgaggtcggtggttcgagtccactaaggcccaccattatcaacacatgagtgttgatcccaTTAAGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGCAAGCAGGATGTCAGCGGTTCGAtcccgcttggc                                                                                                       >  2:396223‑M1/68‑135 (MQ=255)                                  taagggtgaggtcggtggttcgagtccactaaggcccaccattatcaacacatgagtgttgatcccaTTAAGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGCAAGCAGGAGGTCAGCGGTTCGAtcccgcttggc                                                                                                       >  2:110482‑M1/68‑135 (MQ=255)                                      ggttaggtcggtggttcgagtccactaaggcccaccattatcaacacatgagtgttgatcccaatacGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGCAAGCAGGATGTCAGCGGTTCGATCCCGCTTGGCTcca                                                                                                   <  2:1056233‑M1/73‑1 (MQ=255)                                      ggtgaggtcggtggttcgagtccactaaggcccaccattatcaacacatgagtgttgatcccaatacGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGCAAGCAGGAGGTCAGCGGTTCGAtcccgcttggc                                                                                                       <  2:202062‑M1/69‑6 (MQ=255)                                      ggtgaggtcggtggttcgagtccactaaggcccaccattatcaacacatgagtgttgatcccaatacGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGCAAGCAGGAGGTCAGCGGTTCGAtcccgcttggc                                                                                                       >  1:202062‑M1/68‑131 (MQ=255)                                      ggtgaggtcggtggttcgagtccactaaggcccaccattatcaacacatgagtgttgatcccaatacGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGCAAGCAGGAGGTCAGCGGTTCGATCCCGCTTGGCTcca                                                                                                   <  1:101290‑M1/73‑1 (MQ=255)                                      ggtgaggtcggtggttcgagtccactaaggcccaccattatcaacacatgagtgttgatcccaatacGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGCAAGCAGGAGGTCAGCGGTTCGATCCCGCTTGGCTcca                                                                                                   <  2:725310‑M1/73‑1 (MQ=255)                                      ggtgaggtcggtggttcgagtccactaaggcccaccattatcaacacatgagtgttgatcccaatacGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGCAAGCAGGAGGTCAGCGGTTCGATCCCGCTTGGCTcca                                                                                                   <  2:529010‑M1/73‑1 (MQ=255)                                      ggtgaggtcggtggttcgagtccactaaggcccaccattatcaacacatgagtgttgatcccaatacGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGCAAGCAGGAGGTCAGCGGTTCGATCCCGCTTGGCTcca                                                                                                   <  1:1235344‑M1/73‑1 (MQ=255)                                      ggtgaggtcggtggttcgagtccactaaggcccaccattatcaacacatgagtgttgatcccaatacGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGCAAGCAGGAGGTCAGCGGTTCGATCCCGCTTGGCTcca                                                                                                   <  2:1209749‑M1/73‑1 (MQ=255)                                      ggtgaggtcggtggttcgagtccactaaggcccaccattatcaacacatgagtgttgatcccaTTAAGGGGCCATAGCTCAGCTGGGATAGCGCCTGCCTTGCAAGCAGGAGGTCAGCGGTTCGATCCCGCTTGGCTcca                                                                                                   <  2:922173‑M1/77‑1 (MQ=255)                                      ggtgaggtcggtggttcgagtccactaaggcccaccattatcaacacatgagtgttgatcccaTTAAGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGCAAGCAGGAGGTCAGCGGTTCGATcc                                                                                                               <  1:533366‑M1/65‑1 (MQ=255)                                      ggtgaggtcggtggttcgagtccactaaggcccaccattatcaacacatgagtgttgatcccaTTAAGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGCAAGCAGGAGGTCAGCGGTTCGATcc                                                                                                               >  2:533366‑M1/64‑128 (MQ=255)                                      ggtgaggtcggtggttcgagtccactaaggcccaccattatcaacacatgagtgttgatcccaTTAAGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGCAAGCAGGAGGTCAGCGGTTCGATCCCGCTTGGCTcca                                                                                                   <  1:524313‑M1/77‑1 (MQ=255)                                        tgaggtcggtggttcgagtccactaaggcccaccattatcaacacatgagtgttgatcccaatacGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGCAAGCAGGAGGTCAGCGGTTCGATCCCGCTTGGctccatt                                                                                                 <  1:243179‑M1/75‑3 (MQ=255)                                        tgaggtcggtggttcgagtccactaaggcccaccattatcaacacatgagtgttgatcccaTTAAGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGCAAGCAGGAGGTCAGCGGTTCGATCCCGCTTGGctccatt                                                                                                 <  2:508768‑M1/79‑3 (MQ=255)                                        tgaggtcggtggttcgagtccactaaggcccaccattatcaacacatgagtgttgatcccaTTAAGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGCAAGCAGGAGGTCAGCGGTTCGATCCCGCTTGGctccatt                                                                                                 <  1:1444867‑M1/79‑3 (MQ=255)                                        tgaggtcggtggttcgagtccactaaggcccaccattatcaacacatgagtgttgatcccaTTAAGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGCAAGCAGGAGGTCAGCGGTTCGATCCCGCTTGGctccatt                                                                                                 <  2:283561‑M1/79‑3 (MQ=255)                                        tgaggtcggtggttcgagtccactaaggcccaccattatcaacacatgagtgttgatcccaTTAAGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGCAAGCAGGAGGTCAGCGGTTAGATCCCGCTTGGctccatt                                                                                                 <  2:36239‑M1/79‑3 (MQ=255)                                         gaggtcggtggttcgagtccactaaggcccaccattatcaacacatgagtgttgatcccaatacGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGCAAGCAGGAGGTCAGCGGTTCGATc                                                                                                                >  1:50965‑M1/65‑124 (MQ=255)                                         gaggtcggtggttcgagtccactaaggcccaccattatcaacacatgagtgttgatcccaatacGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGCAAGCAGGAGGTCAGCGGTTCGATc                                                                                                                <  2:50965‑M1/60‑1 (MQ=255)                                          aggtcggtggttcgagtccactaaggcccaccattatcaacacatgagtgttgatcccaataaGGGCC‑ATAGCTCAGCTGGGAGAGCGCCTGCCTTGCAAGCAGGAGGTCAGCGGTTCGATCCCGCTtggctccatttgt                                                                                              <  2:1147873‑M1/77‑6 (MQ=255)                                          aggtcggtggttcgagtccactaaggcccaccattatcaacacatgagtgttgatcccaTTAAGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGCAAGCAGGAGGTCAGCGGTTCGATCCCGCTTggctccatttg                                                                                               <  2:1549939‑M1/81‑5 (MQ=255)                                           ggtcggtggttcgagtccactaaggcccaccattatcaacacatgagtgttgatcccaatacGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGCAAGCAGGAGGTCAGCGGTTCGATCCCGCTtggctccatttgt                                                                                              >  2:436110‑M1/63‑135 (MQ=255)                                           ggtcggtggttcgagtccactaaggcccaccattatcaacacatgagtgttgatcccaatacGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGCAAGCAGGAGGTCAGCGGTTCGATCCCGCTtggctccatttgt                                                                                              >  2:101290‑M1/63‑135 (MQ=255)                                           ggtcggtggttcgagtccactaaggcccaccattatcaacacatgagtgttgatcccaatacGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGCAAGCAGGAGGTCAGCGGTTCGATCCCGCTtggctccatttgt                                                                                              >  2:243179‑M1/63‑135 (MQ=255)                                           ggtcggtggttcgagtccactaaggcccaccattatcaacacatgagtgttgatcccaTTAAGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGCAAGCAGGAGGTCAGCGGTTCGATCCCGCTtggctccatttgt                                                                                              <  2:1114860‑M1/82‑6 (MQ=255)                                             tcggtggttcgagtccactaaggcccaccattatcaacacatgagtgttgatcccaTTAAGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGCAAGCAGGAGGTCAGCGGTTCGATCCCGcttggctccatttgttc                                                                                            >  1:1159044‑M1/57‑133 (MQ=255)                                                gtggttcgagtccactaaggcccaccattatcaacacatgagtgttgatcccaatacGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGCAAGCAGGAGGTCAGCGGTTCGATCCcgctt                                                                                                          >  1:948940‑M1/58‑121 (MQ=255)                                                gtggttcgagtccactaaggcccaccattatcaacacatgagtgttgatcccaatacGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGCAAGCAGGAGGTCAGCGGTTCGATCCcgctt                                                                                                          <  2:948940‑M1/66‑3 (MQ=255)                                                       gCCTACCAAATTTGCACGGCAAATTTGAAGAGGTTTTAACTACATGTTATGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCTTTGTACGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCACCATCTCTGTAGTGGtt                                                                                  >  1:1830812/1‑140 (MQ=17)                                                                aTTTGCACGGCAAATTTGAAGAGGTTTTAACTACATGTTATGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCACCATCTCTGTAGTGGTTAAATaaaaa                                                                         <  1:1810169/140‑1 (MQ=33)                                                                      aCGGCAAATTTGAAGAGGTTTTAACTACATGTTATGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCACCATCTCTGTAGTGGTTAAATAAAAAATACtt                                                                   <  1:2316965/140‑1 (MQ=33)                                                                                       gTTTTAACTACATGTTATGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCACCATCTCTGTAGTGGTTAAATAAAAAATACTTCAGAGTGTACCTGCaaa                                                  >  1:1689869/1‑140 (MQ=32)                                                                                           tAACTACATGTTATGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCACCATCTCTGTAGTGGTTAAATAAAAAATTCTTCAGAGTGTACCTGCAAAGGtt                                              <  1:1611121/140‑1 (MQ=17)                                                                                                  aTGTTATGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCCCCATCTCTGTAGTGGTTAAATAAAAAATACTTCAGAGTGTACCTGCAAAGGTTCACTGCg                                       <  2:1897241/140‑1 (MQ=17)                                                                                                  aTGTTATGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCACCATCTCTGTAGTGGTTAAATAAAAAATACTTCAGAGTGTACCTGCAAAGGTTCACTGCg                                       <  2:2161450/140‑1 (MQ=33)                                                                                                       aTGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCACCATCTCTGTAGTGGTTAAATAAAAAATACTTCAGAGTGTACCTGCAAAGGTTCACTGCGAAGtt                                  <  2:2093185/140‑1 (MQ=33)                                                                                                           ggCTATAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCACCATCTCTGTAGTGGTTAAATAAAAAATACTTCAGAGTGTACCTGCAAAGGTTCACTGCGAAGTTTTGc                              >  1:2297627/1‑140 (MQ=32)                                                                                                            gCTATAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCACCATCTCTGTAGTGGTTAAATAAAAAATACTTCAGAGTGTTCCTGCAAAGGTTCACTGCGAAGTTTTGct                             <  2:1968840/140‑1 (MQ=17)                                                                                                                taGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCACCATCTCTGTAGTGGTTAAATAAAAAATACTTCAGAGTGTACCTGCAAAGGTTCACTGCGAAGTTTTGCTCttt                         >  2:2022159/1‑140 (MQ=32)                                                                                                                taGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCACCATCTCTGTAGTGGTTAAATAAAAAATACTTCAGAGTGTACCTGCAAAGGTTCACTGCGAAGTTTTACTCttt                         >  2:2120325/1‑140 (MQ=17)                                                                                                                        cTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCACCATCTCTGTAGTGGTTAAATAAAAAATACTTCAGAGTGTACCTGCAAAGGTTCACTGCGAAGTTTTGCTCTTTAAAAATCt                 >  1:1692765/1‑140 (MQ=32)                                                                                                                          gggAGAGCGCCTGCTTTGCACGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCACCATCTCTGTAGTGGTTAAATAAAAAATACTTCAGAGTGTACCTGCAAAGGTTCACTGCGAAGTTTTGCTCTTTAAAAATCTgg               >  2:1868062/1‑140 (MQ=32)                                                                                                                             agagCGCCTGCTTTGCACGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCACCATCTCTGTAGTGGTTAAATAAAAAATACTTCAGAGTGTACCTGCAAAGGTTCACTGCGAAGTTTTGCTCTTTAAAAATCTGGATc            <  1:1811855/140‑1 (MQ=32)                                                                                                                                gcgcCTGCTTTGCACGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCACCATCTCTGTAGTGGTTAAATAAAAAATACTTCAGAGTGTACCTGCAAAGGTTCACTGCGAAGTTTTGCTCTTTAAAAATCTGGATCAAg         >  2:2251868/1‑140 (MQ=32)                                                                                                                                   ccTGCTTTGCACGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCACCATCTCTGTAGTGGTTAAATAAAAAATACTTCAGAGTGTACCTGCAAAGGTTCACTGCGAAGTTTTGCTCTTTAAAAATCTGGATCAAGCTg      >  1:1763060/1‑140 (MQ=32)                                                                                                                                   ccTGCTTTGCACGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCACCATCTCTGTAGTGGTTAAATAAAAAATACTTCAGAGTGTACCTGCAAAGGTTCACTGCGAAGTTTTGCTCTTTAAAAATCTGGATCAAGCTg      <  2:1689869/140‑1 (MQ=32)                                                                                                                                       cTTTGCACGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCACCATCTCTGTAGTGGTTAAATAAAAAATACTTCAGAGTGTACCTGCAAAGGTTCACTGCGAAGTTTTGCTCTTTAAAAATCTGGATCAAGCTGaaaa  >  2:1795108/1‑140 (MQ=32)                                                                                                                                        |                                                                                                                                           GTGGTTAGAGCGCACCCCTGATAAGGGTGAGGTCGGTGGTTCAAGTCCACTCAGGCCTACCAAATTTGCACGGCAAATTTGAAGAGGTTTTAACTACATGTTATGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCACCATCTCTGTAGTGGTTAAATAAAAAATACTTCAGAGTGTACCTGCAAAGGTTCACTGCGAAGTTTTGCTCTTTAAAAATCTGGATCAAGCTGAAAA  >  NC_000913/225396‑225669

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑