Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	225,537	C→A	noncoding (38/76 nt)	alaV →	tRNA‑Ala

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	225,537	0	C	A	76.8%	184.3 / 64.4	82	noncoding (38/76 nt)	alaV	tRNA‑Ala
Reads supporting (aligned to +/- strand):  ref base C (10/9);  new base A (31/32);  total (41/41)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

AGAGCGCACCCCTGATAAGGGTGAGGTCGGTGGTTCAAGTCCACTCAGGCCTACCAAATTTGCACGGCAAATTTGAAGAGGTTTTAACTACATGTTATGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCACCATCTCTGTAGTGGTTAAATAAAAAATACTTCAGAGTGTACCTGCAAAGGTTCACTGCGAAGTTTTGCTCTTTAAAAATCTGGATCAAGCTGAAAATTG  >  NC_000913/225402‑225672                                                                                                                                        |                                                                                                                                        gctcagctggttagagcgcacccctgataagggtgaggtcggtggttcgagtccactaaggcccaccattatcaacacatgagtgttgatcccaatacGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGCAAGCag                                                                                                                                     >  2:249496‑M1/99‑140 (MQ=255)   tcagctggttagagcgcacccctgataagggtgaggtcggtggttctagtccactaaggcccaccattatcaacacatgagtgttgatcccaatacGGGGCCATAGCTCAGCTGCGAGAGCGCCTGCCTTGCAAGCagga                                                                                                                                   >  1:1411760‑M1/97‑140 (MQ=255)   tcagctggttagagcgcacccctgataagggtgaggtcggtggttcgagtccactaaggcccaccattatcaacacatgagtgttgatcccaatacGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGCAAGCagga                                                                                                                                   >  2:19965‑M1/97‑140 (MQ=255)    cagctggttagagcgcacccctgataagggtgaggtcggtggttcgagtccactaaggcccaccattatcaacacatgagtgttgatcccaatacGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGCAAGCaggag                                                                                                                                  <  1:1294063‑M1/45‑1 (MQ=255)     agctggttagagcgcacccctgataagggtgaggtcggtggttcgagtccactaaggcccaccattatcaacacatgagtgttgatcccaTTAAGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGCAAGCaggagg                                                                                                                                 <  2:1384288‑M1/50‑1 (MQ=255)       ctggttagagcgcacccctgataagggtgaggtcggtggttcgagtccactaaggcccaccattatcaacacatgagtgttgatcccaatacGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGCAAGCAGGAGGTc                                                                                                                               >  1:1451511‑M1/93‑140 (MQ=255)        tggttagagcgcacccctgataagggtgaggtcggtggttcgagtccactaaggcccaccattatcaacacatgagtgttgatcccaatacGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGCAAGCAGGAGGtca                                                                                                                              >  2:2098445‑M1/92‑139 (MQ=255)         ggttagagcgcacccctgataagggtgaggtcggtggttcgagtccactaaggcccaccattatcaacacatgagtgttgatcccaatacGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGCAAGCAGGAGgtcag                                                                                                                             >  1:255996‑M1/91‑138 (MQ=255)         ggttagagcgcacccctgataagggtgaggtcggtggttcgagtccactaaggcccaccattatcaacacatgagtgttgatcccaatacGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGCAAGCAGGAGgtcag                                                                                                                             >  1:726719‑M1/91‑138 (MQ=255)         ggttagagcgcacccctgataagggtgaggtcggtggttcgagtccactaaggcccaccattatcaacacatgagtgttgatcccaatacGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGCAAGCAGGAGgtcag                                                                                                                             >  2:55879‑M1/91‑138 (MQ=255)         ggttagagcgcacccctgataagggtgaggtcggtggttcgagtccactaaggcccaccattatcaacacatgagtgttgatcccaatacGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGCAAGCAGGAGgtcag                                                                                                                             >  1:815255‑M1/91‑138 (MQ=255)         ggttagagcgcacccctgataagggtgaggtcggtggttcgagtccactaaggcccaccattatcaacacatgagtgttgatcccaTTAAGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGCAAGCAGGAGgtcag                                                                                                                             >  1:1372127‑M1/87‑138 (MQ=255)               agcgcacccctgataagggtgaggtcggtggttcgagtccactaaggcccaccattatcaacacatgagtgttgatcccaTTAAGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGCAAGCAGGAGGTCAGCGGTTc                                                                                                                       <  1:1376237‑M1/60‑1 (MQ=255)                 cgcacccctgataagggtgaggtcggtggttcgagtccactaaggcccaccattatcaacacatgagtgtttatcccaatacGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGCAAGCAGGAGGTCAGCGGTTCGa                                                                                                                     <  2:191391‑M1/58‑1 (MQ=255)                 cgcacccctgataagggtgaggtcggtggttcgagtccactaaggcccaccattatcaacacatgagtgttgatcccaatacGGGGCCATAGCTCAGCTGGGATAGCGCCTGCCTTGCAAGCAGGAGGTCAGcggttaga                                                                                                                     <  2:1186710‑M1/58‑4 (MQ=255)                 cgcacccctgataagggtgaggtcggtggttcgagtccactaaggcccaccattatcaacacatgagtgttgatcccaatacGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGCAAGCAGGAGGTCAGCGGTTCGa                                                                                                                     <  1:213827‑M1/58‑1 (MQ=255)                 cgcacccatgataagggtgaggtcggtggttcgagtccactaaggcccaccattatcaacacatgagtgttgatcccaTTAAGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGCAAGCAGGAGGTCAGCGGTTCGa                                                                                                                     <  1:881194‑M1/62‑1 (MQ=255)                  gcacccctgataagggtgaggtcggtggttcgagtccactaaggcccaccattatcaacacatgagtgttgatcccaTTAAGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGCAAGCAGGAGGTCAGCGGTTCGAt                                                                                                                    >  2:435597‑M1/78‑140 (MQ=255)                   cacccctgataagggtgaggtcggtggttcgagtccactaaggcccaccattatcaacacatgagtgttgatcccaatacGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGCAAGCAGGAGGTCAGCGGTTCGATc                                                                                                                   >  2:1294063‑M1/81‑140 (MQ=255)                   cacccctgataagggtgaggtcggtggttcgagtccactaaggcccaccattatcaacacatgagtgttgatcccaatacGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGCAAGCAGGAGGTCAGCGGTTCGATc                                                                                                                   >  1:384781‑M1/81‑140 (MQ=255)                    acccctgataagggtgaggtcggtggttcgagtccactaaggcccaccattatcaacacatgagtgttgatcccaatacGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGCAAGCAGGAGgtcag                                                                                                                             >  1:1171190‑M1/80‑127 (MQ=255)                    acccctgataagggtgaggtcggtggttcgagtccactaaggcccaccattatcaacacatgagtgttgatcccaatacGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGCAAGCAGGAGgtcag                                                                                                                             <  2:1171190‑M1/50‑3 (MQ=255)                      ccctgataagggtgaggtcggtggttcgagtccactaaggcccaccattatcaacacatgagtgttgatcccaatacGGGGCCATAGCTCAGCTGGGATAGCGCCTGCCTTGCAAGCAGGAGGTCAGCGGTTAGATCCCg                                                                                                                >  1:1186710‑M1/78‑140 (MQ=255)                      ccctgataagggtgaggtcggtggttcgagtccactaaggcccaccattatcaacacatgagtgttgatcccaatacGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGCAAGCAGGAGGTCAGCGGTTCGATCCCg                                                                                                                <  1:983467‑M1/63‑1 (MQ=255)                      ccctgataagggtgaggtaggtggttcgagtccactaaggcccaccattatcaacacatgagtgttgatcccaatacGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGCAAGCAGGAGGTCAGCGGTTCGATCCCg                                                                                                                <  1:531534‑M1/63‑1 (MQ=255)                       cctgataagggtgaggtcggtggttcgagtccactaaggcccaccattatcaacacatgagtgttgatcccaatacGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGCAAGCAGGAGGTCAGCGGTTCGATCCCGc                                                                                                               >  2:983467‑M1/77‑140 (MQ=255)                       cctgataagggtgaggtcggtggttcgagtccactaaggcccaccattatcaacacatgagtgttgatcccaTTAAGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCATTGCAAGCAGGAGGTCAGCGGTTCGATCCCGc                                                                                                               >  1:673176‑M1/73‑140 (MQ=255)                         tgataagggtgaggtcggtggttcgagtccactaaggcccaccattatcaacacatgagtgttgatcccaatacGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGCAAGCAGGAGGTCAGTGGTTCGATCCcgctt                                                                                                             >  1:888155‑M1/75‑138 (MQ=255)                         tgataagggtgaggtcggtggttcgagtccactaaggcccaccattatcaacacatgagtgttgatcccaatacGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGCAAGCAGGAGGTCAGCGGTTCGATCCcgctt                                                                                                             >  2:104934‑M1/75‑138 (MQ=255)                         tgataagggtgaggtcggtggttcgagtccactaaggcccaccattatcaacacatgagtgttgatcccaatacGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGCAAGCAGGAGGTCAGCGGTTCGATCCcgctt                                                                                                             >  1:235358‑M1/75‑138 (MQ=255)                         tgataagggtgaggtcggtggttcgagtccactaaggcccaccattatcaacacatgagtgttgatcccaatacGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGCAAGCAGGAGGTAAGCGGTTCGATCCcgctt                                                                                                             >  2:704249‑M1/75‑138 (MQ=255)                           ataagggtgaggtcggtggttcgagtccactaaggcccaccattatcaacacatgagtgttgatcccaatacGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGCAAGCAGGAGGTCAGCGGTTCGATcccgcttgg                                                                                                           <  2:77029‑M1/68‑5 (MQ=255)                           ataagggtgaggtcggtggttcgagtccactaaggcccaccattatcaacacatgagtgttgatcccaTTAAGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGCAAGCAGGAGGTCAGCGGTTCGATcccgcttgg                                                                                                           <  1:540404‑M1/72‑5 (MQ=255)                            taatggtgaggtcggtggttcgagtccactaaggcccaccattatcaacacatgagtgttgatcccaTTAAGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGCAAGCAGGATGTCAGCGGTTCGAtcccgcttggc                                                                                                          >  2:396223‑M1/68‑135 (MQ=255)                            taagggtgaggtcggtggttcgagtccactaaggcccaccattatcaacacatgagtgttgatcccaTTAAGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGCAAGCAGGAGGTCAGCGGTTCGAtcccgcttggc                                                                                                          >  2:110482‑M1/68‑135 (MQ=255)                                ggttaggtcggtggttcgagtccactaaggcccaccattatcaacacatgagtgttgatcccaatacGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGCAAGCAGGATGTCAGCGGTTCGATCCCGCTTGGCTcca                                                                                                      <  2:1056233‑M1/73‑1 (MQ=255)                                ggtgaggtcggtggttcgagtccactaaggcccaccattatcaacacatgagtgttgatcccaatacGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGCAAGCAGGAGGTCAGCGGTTCGAtcccgcttggc                                                                                                          >  1:202062‑M1/68‑131 (MQ=255)                                ggtgaggtcggtggttcgagtccactaaggcccaccattatcaacacatgagtgttgatcccaatacGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGCAAGCAGGAGGTCAGCGGTTCGAtcccgcttggc                                                                                                          <  2:202062‑M1/69‑6 (MQ=255)                                ggtgaggtcggtggttcgagtccactaaggcccaccattatcaacacatgagtgttgatcccaatacGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGCAAGCAGGAGGTCAGCGGTTCGATCCCGCTTGGCTcca                                                                                                      <  2:529010‑M1/73‑1 (MQ=255)                                ggtgaggtcggtggttcgagtccactaaggcccaccattatcaacacatgagtgttgatcccaatacGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGCAAGCAGGAGGTCAGCGGTTCGATCCCGCTTGGCTcca                                                                                                      <  2:725310‑M1/73‑1 (MQ=255)                                ggtgaggtcggtggttcgagtccactaaggcccaccattatcaacacatgagtgttgatcccaatacGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGCAAGCAGGAGGTCAGCGGTTCGATCCCGCTTGGCTcca                                                                                                      <  1:1235344‑M1/73‑1 (MQ=255)                                ggtgaggtcggtggttcgagtccactaaggcccaccattatcaacacatgagtgttgatcccaatacGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGCAAGCAGGAGGTCAGCGGTTCGATCCCGCTTGGCTcca                                                                                                      <  2:1209749‑M1/73‑1 (MQ=255)                                ggtgaggtcggtggttcgagtccactaaggcccaccattatcaacacatgagtgttgatcccaatacGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGCAAGCAGGAGGTCAGCGGTTCGATCCCGCTTGGCTcca                                                                                                      <  1:101290‑M1/73‑1 (MQ=255)                                ggtgaggtcggtggttcgagtccactaaggcccaccattatcaacacatgagtgttgatcccaTTAAGGGGCCATAGCTCAGCTGGGATAGCGCCTGCCTTGCAAGCAGGAGGTCAGCGGTTCGATCCCGCTTGGCTcca                                                                                                      <  2:922173‑M1/77‑1 (MQ=255)                                ggtgaggtcggtggttcgagtccactaaggcccaccattatcaacacatgagtgttgatcccaTTAAGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGCAAGCAGGAGGTCAGCGGTTCGATcc                                                                                                                  <  1:533366‑M1/65‑1 (MQ=255)                                ggtgaggtcggtggttcgagtccactaaggcccaccattatcaacacatgagtgttgatcccaTTAAGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGCAAGCAGGAGGTCAGCGGTTCGATcc                                                                                                                  >  2:533366‑M1/64‑128 (MQ=255)                                ggtgaggtcggtggttcgagtccactaaggcccaccattatcaacacatgagtgttgatcccaTTAAGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGCAAGCAGGAGGTCAGCGGTTCGATCCCGCTTGGCTcca                                                                                                      <  1:524313‑M1/77‑1 (MQ=255)                                  tgaggtcggtggttcgagtccactaaggcccaccattatcaacacatgagtgttgatcccaatacGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGCAAGCAGGAGGTCAGCGGTTCGATCCCGCTTGGctccatt                                                                                                    <  1:243179‑M1/75‑3 (MQ=255)                                  tgaggtcggtggttcgagtccactaaggcccaccattatcaacacatgagtgttgatcccaTTAAGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGCAAGCAGGAGGTCAGCGGTTCGATCCCGCTTGGctccatt                                                                                                    <  2:508768‑M1/79‑3 (MQ=255)                                  tgaggtcggtggttcgagtccactaaggcccaccattatcaacacatgagtgttgatcccaTTAAGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGCAAGCAGGAGGTCAGCGGTTCGATCCCGCTTGGctccatt                                                                                                    <  2:283561‑M1/79‑3 (MQ=255)                                  tgaggtcggtggttcgagtccactaaggcccaccattatcaacacatgagtgttgatcccaTTAAGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGCAAGCAGGAGGTCAGCGGTTCGATCCCGCTTGGctccatt                                                                                                    <  1:1444867‑M1/79‑3 (MQ=255)                                  tgaggtcggtggttcgagtccactaaggcccaccattatcaacacatgagtgttgatcccaTTAAGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGCAAGCAGGAGGTCAGCGGTTAGATCCCGCTTGGctccatt                                                                                                    <  2:36239‑M1/79‑3 (MQ=255)                                   gaggtcggtggttcgagtccactaaggcccaccattatcaacacatgagtgttgatcccaatacGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGCAAGCAGGAGGTCAGCGGTTCGATc                                                                                                                   >  1:50965‑M1/65‑124 (MQ=255)                                   gaggtcggtggttcgagtccactaaggcccaccattatcaacacatgagtgttgatcccaatacGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGCAAGCAGGAGGTCAGCGGTTCGATc                                                                                                                   <  2:50965‑M1/60‑1 (MQ=255)                                    aggtcggtggttcgagtccactaaggcccaccattatcaacacatgagtgttgatcccaataaGGGCC‑ATAGCTCAGCTGGGAGAGCGCCTGCCTTGCAAGCAGGAGGTCAGCGGTTCGATCCCGCTtggctccatttgt                                                                                                 <  2:1147873‑M1/77‑6 (MQ=255)                                    aggtcggtggttcgagtccactaaggcccaccattatcaacacatgagtgttgatcccaTTAAGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGCAAGCAGGAGGTCAGCGGTTCGATCCCGCTTggctccatttg                                                                                                  <  2:1549939‑M1/81‑5 (MQ=255)                                     ggtcggtggttcgagtccactaaggcccaccattatcaacacatgagtgttgatcccaatacGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGCAAGCAGGAGGTCAGCGGTTCGATCCCGCTtggctccatttgt                                                                                                 >  2:436110‑M1/63‑135 (MQ=255)                                     ggtcggtggttcgagtccactaaggcccaccattatcaacacatgagtgttgatcccaatacGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGCAAGCAGGAGGTCAGCGGTTCGATCCCGCTtggctccatttgt                                                                                                 >  2:101290‑M1/63‑135 (MQ=255)                                     ggtcggtggttcgagtccactaaggcccaccattatcaacacatgagtgttgatcccaatacGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGCAAGCAGGAGGTCAGCGGTTCGATCCCGCTtggctccatttgt                                                                                                 >  2:243179‑M1/63‑135 (MQ=255)                                     ggtcggtggttcgagtccactaaggcccaccattatcaacacatgagtgttgatcccaTTAAGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGCAAGCAGGAGGTCAGCGGTTCGATCCCGCTtggctccatttgt                                                                                                 <  2:1114860‑M1/82‑6 (MQ=255)                                       tcggtggttcgagtccactaaggcccaccattatcaacacatgagtgttgatcccaTTAAGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGCAAGCAGGAGGTCAGCGGTTCGATCCCGcttggctccatttgttc                                                                                               >  1:1159044‑M1/57‑133 (MQ=255)                                          gtggttcgagtccactaaggcccaccattatcaacacatgagtgttgatcccaatacGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGCAAGCAGGAGGTCAGCGGTTCGATCCcgctt                                                                                                             >  1:948940‑M1/58‑121 (MQ=255)                                          gtggttcgagtccactaaggcccaccattatcaacacatgagtgttgatcccaatacGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGCAAGCAGGAGGTCAGCGGTTCGATCCcgctt                                                                                                             <  2:948940‑M1/66‑3 (MQ=255)                                                 gCCTACCAAATTTGCACGGCAAATTTGAAGAGGTTTTAACTACATGTTATGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCTTTGTACGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCACCATCTCTGTAGTGGtt                                                                                     >  1:1830812/1‑140 (MQ=17)                                                          aTTTGCACGGCAAATTTGAAGAGGTTTTAACTACATGTTATGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCACCATCTCTGTAGTGGTTAAATaaaaa                                                                            <  1:1810169/140‑1 (MQ=33)                                                                aCGGCAAATTTGAAGAGGTTTTAACTACATGTTATGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCACCATCTCTGTAGTGGTTAAATAAAAAATACtt                                                                      <  1:2316965/140‑1 (MQ=33)                                                                                 gTTTTAACTACATGTTATGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCACCATCTCTGTAGTGGTTAAATAAAAAATACTTCAGAGTGTACCTGCaaa                                                     >  1:1689869/1‑140 (MQ=32)                                                                                     tAACTACATGTTATGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCACCATCTCTGTAGTGGTTAAATAAAAAATTCTTCAGAGTGTACCTGCAAAGGtt                                                 <  1:1611121/140‑1 (MQ=17)                                                                                            aTGTTATGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCCCCATCTCTGTAGTGGTTAAATAAAAAATACTTCAGAGTGTACCTGCAAAGGTTCACTGCg                                          <  2:1897241/140‑1 (MQ=17)                                                                                            aTGTTATGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCACCATCTCTGTAGTGGTTAAATAAAAAATACTTCAGAGTGTACCTGCAAAGGTTCACTGCg                                          <  2:2161450/140‑1 (MQ=33)                                                                                                 aTGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCACCATCTCTGTAGTGGTTAAATAAAAAATACTTCAGAGTGTACCTGCAAAGGTTCACTGCGAAGtt                                     <  2:2093185/140‑1 (MQ=33)                                                                                                     ggCTATAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCACCATCTCTGTAGTGGTTAAATAAAAAATACTTCAGAGTGTACCTGCAAAGGTTCACTGCGAAGTTTTGc                                 >  1:2297627/1‑140 (MQ=32)                                                                                                      gCTATAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCACCATCTCTGTAGTGGTTAAATAAAAAATACTTCAGAGTGTTCCTGCAAAGGTTCACTGCGAAGTTTTGct                                <  2:1968840/140‑1 (MQ=17)                                                                                                          taGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCACCATCTCTGTAGTGGTTAAATAAAAAATACTTCAGAGTGTACCTGCAAAGGTTCACTGCGAAGTTTTGCTCttt                            >  2:2022159/1‑140 (MQ=32)                                                                                                          taGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCACCATCTCTGTAGTGGTTAAATAAAAAATACTTCAGAGTGTACCTGCAAAGGTTCACTGCGAAGTTTTACTCttt                            >  2:2120325/1‑140 (MQ=17)                                                                                                                  cTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCACCATCTCTGTAGTGGTTAAATAAAAAATACTTCAGAGTGTACCTGCAAAGGTTCACTGCGAAGTTTTGCTCTTTAAAAATCt                    >  1:1692765/1‑140 (MQ=32)                                                                                                                    gggAGAGCGCCTGCTTTGCACGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCACCATCTCTGTAGTGGTTAAATAAAAAATACTTCAGAGTGTACCTGCAAAGGTTCACTGCGAAGTTTTGCTCTTTAAAAATCTgg                  >  2:1868062/1‑140 (MQ=32)                                                                                                                       agagCGCCTGCTTTGCACGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCACCATCTCTGTAGTGGTTAAATAAAAAATACTTCAGAGTGTACCTGCAAAGGTTCACTGCGAAGTTTTGCTCTTTAAAAATCTGGATc               <  1:1811855/140‑1 (MQ=32)                                                                                                                          gcgcCTGCTTTGCACGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCACCATCTCTGTAGTGGTTAAATAAAAAATACTTCAGAGTGTACCTGCAAAGGTTCACTGCGAAGTTTTGCTCTTTAAAAATCTGGATCAAg            >  2:2251868/1‑140 (MQ=32)                                                                                                                             ccTGCTTTGCACGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCACCATCTCTGTAGTGGTTAAATAAAAAATACTTCAGAGTGTACCTGCAAAGGTTCACTGCGAAGTTTTGCTCTTTAAAAATCTGGATCAAGCTg         >  1:1763060/1‑140 (MQ=32)                                                                                                                             ccTGCTTTGCACGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCACCATCTCTGTAGTGGTTAAATAAAAAATACTTCAGAGTGTACCTGCAAAGGTTCACTGCGAAGTTTTGCTCTTTAAAAATCTGGATCAAGCTg         <  2:1689869/140‑1 (MQ=32)                                                                                                                                 cTTTGCACGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCACCATCTCTGTAGTGGTTAAATAAAAAATACTTCAGAGTGTACCTGCAAAGGTTCACTGCGAAGTTTTGCTCTTTAAAAATCTGGATCAAGCTGaaaa     >  2:1795108/1‑140 (MQ=32)                                                                                                                                    tgtaCGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCACCATCTCTGTAGTGGTTAAATAAAAAATACTTCAGAGTGTACCTGCAAAGGTTCACTGCGAAGTTTTGCTCTTTAAAAATCTGGATCAAGCTTAAAATTg  <  2:1830812/137‑1 (MQ=17)                                                                                                                                        |                                                                                                                                        AGAGCGCACCCCTGATAAGGGTGAGGTCGGTGGTTCAAGTCCACTCAGGCCTACCAAATTTGCACGGCAAATTTGAAGAGGTTTTAACTACATGTTATGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCACCATCTCTGTAGTGGTTAAATAAAAAATACTTCAGAGTGTACCTGCAAAGGTTCACTGCGAAGTTTTGCTCTTTAAAAATCTGGATCAAGCTGAAAATTG  >  NC_000913/225402‑225672

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑