Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,052,605	T→G	D253A (GAC→GCC)	yccM ←	putative 4Fe‑4S membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,052,605	0	T	G	100.0%	174.0 / NA	47	D253A (GAC→GCC)	yccM	putative 4Fe‑4S membrane protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (23/24);  total (23/24)

GTGAAAAGGTCAATGTTGAGGCTACCGGACATGACTCTACGCAAGTCATACAGCCGGTACATTCCACTGTTCGTACCTGAATTAATTTATCGACCGGGATCCGTGATGGGCAATTTTTTGCGCATTTGCCACAGTCGATACAACTTTCGGCATTGCGACGAATCTTAAACGGCGACAATAGCGAAACCACGCCCATCAGCGCGCCATATGGGCAAAGATAACGACACCAGGCATGGCGAATAAACAGGCTGGCAATCAGCAAAACGGTC  >  NC_000913/1052471‑1052739                                                                                                                                       |                                                                                                                                       gTGAAAAGGTCAATGTTGAGGCTACCGGACATGACTCTACGCAAGTCATACAGCCGGTACATTCCACTGTTCGTACCTGAATTAATTTATCGACCGGGATCCGTGATGGGCAATTTTTTGCGCATTTGCCACAGGCGATa                                                                                                                                   <  2:1819047/140‑1 (MQ=255)   gAAAAGGTCAATGTTGAGGCTACCGGACATGACTCTACGCAAGTCATACAGCCGGTACATTCCACTGTTCGTACCTGAATTAATTTATCGACCGGGATCCGTGATGGGCAATTTTTTGCGCATTTGCCACAGGCGATACa                                                                                                                                 <  2:2293624/140‑1 (MQ=255)                  gAGGCTACCGGACATGACTCTACGCAAGTCATACAGCCGGTACATTCCACTGTTCGTACCTGAATTAATTTATCGACCGGGATCCGTGATGGGCAATTTTTTGCGCATTTGCCACAGGCGATACAACTTTCGGCATTGcg                                                                                                                  >  2:1826531/1‑140 (MQ=255)                   aGGCTACCGGACATGACTCTACGCAAGTCATACAGCCGGTACATTCCACTGTTCGTACCTGAATTAATTTATCGACCGGGATCCGTGATGGGCAATTTTTTGCGCATTTGCCACAGGCGATACAACTTTCGGCATTGcga                                                                                                                 >  1:2015033/1‑140 (MQ=255)                     gCTACCGGACATGACTCTACGCAAGTCATACAGCCGGTACATTCCACTGTTCGTACCTGAATTAATTTATCGACCGGGATCCGTGATGGGCAATTTTTTGCGCATTTGCCACAGGCGATACAACTTTCGGCATTGcgacg                                                                                                               <  2:1919398/140‑1 (MQ=255)                        aCCGGACATGACTCTACGCAAGTCATACAGCCGGTACATTCCACTGTTCGTACCTGGATTAATTTATCGACCGGGATCCGTGATGGGCAATTTTTTGCGCATTTGCCACAGGCGATACAACTTTCGGCATTGCGACGAAt                                                                                                            >  1:1969308/1‑140 (MQ=255)                        aCCGGACATGACTCTACGCAAGTCATACAGCCGGTACATTCCACTGTTCGTACCTGAATTAATTTATCGACCGGGATCCGTGATGGGCAATTTTTTGCGCATTTGCCACAGGCGATACAACTTTCGGCATTGCGACGAAt                                                                                                            >  1:2029539/1‑140 (MQ=255)                        aCCGGACATGACTCTACGCAAGTCATACAGCCGGTACATTCCACTGTTCGTACCTGAATTAATTTATCGACCGGGATCCGTGATGGGCAATTTTTTGCGCATTTGCCACAGGCGATACAACTTTCGGCATTGCGACGAAt                                                                                                            >  1:1899969/1‑140 (MQ=255)                             aCATGACTCTACGCAAGTCATACAGCCGGTACATTCCACTGTTCGTACCTGAATTAATTTATCGACCGGGATCCGTGATGGGCAATTTTTTGCGCATTTGCCACAGGCGATACAACTTTCGGCATTGCGACGAATCTTaa                                                                                                       <  1:2222709/140‑1 (MQ=255)                                     ctACGCAAGTCATACAGCCGGTACATTCCACTGTTCGTACCTGAATTAATTTATCGACCGGGATCCGTGATGGGCAATTTTTTGCGCATTTGCCACAGGCGATACAACTTTCGGCATTGCGACGAATCTTAAACGGCGAc                                                                                               <  1:2293183/140‑1 (MQ=255)                                      tACGCAAGTCATACAGCCGGTACATTCCACTGTTCGTACCTGAATTAATTTATCGACCGGGATCCGTGATGGGCAATTTTTTGCGCATTTGCCACAGGCGATACAACTTTCGGCATTGCGACGAATCTTAAACGGCGACa                                                                                              >  1:1878292/1‑140 (MQ=255)                                      tACGCAAGTCATACAGCCGGTACATTCCACTGTTCGTACCTGAATTAATTTATCGACCGGGATCCGTGATGGGCAATTTTTTGCGCATTTGCCACAGGCGATACAACTTTCGGCATTGCGACGAATCTTAAACGGCGACa                                                                                              >  2:1669374/1‑140 (MQ=255)                                          cAAGTCATACAGCCGGTACATTCCACTGTTCGTACCTGAATTAATTTATCGACCGGGATCCGTGATGGGCAATTTTTTGCGCATTTGCCACAGGCGATACAACTTTCGGCATTGCAACGAATCTTAAACGGCGACAATAg                                                                                          <  1:1976835/140‑1 (MQ=255)                                             gTCATACAGCCGGTACATTCCACTGTTCGTACCTGAATTAATTTATCGACCGGGATCCGTGATGGGCAATTTTTTGCGCATTTGCCACAGGCGATACAACTTTCGGCATTGCGACGAATCTTAAACGGCGACAATAGCGa                                                                                       >  2:1689176/1‑140 (MQ=255)                                               cATACAGCCGGTACATTCCACTGTTCGTACCTGAATTAATTTATCGACCGGGATCCGTGATGGGCAATTTTTTGCGCATTTGCCACAGGCGATACAACTTTCGGCATTGCGACGAATCTTAAACGGCGACAATAGCGaaa                                                                                     <  2:1820541/140‑1 (MQ=255)                                                  aCAGCCGGTACATTCCACTGTTCGTACCTGAATTAATTTATCGACCGGGATCCGTGATGGGCAATTTTTTGCGCATTTGCCACAGGCGATACAACTTTCGGCATTGCGACGAATCTTAAACGGCGACAATAGCGAAACCa                                                                                  <  1:1700529/140‑1 (MQ=255)                                                  aCAGCCGGTACATTCCACTGTTCGTACCTGAATTAATTTATCGACCGGGATCCGTGATGGGCAATTTTTTGCGCATTTGCCACAGGCGATACAACTTTCGGCATTGCGACGAATCTTAAACGGCGACAATAGCGAAACCa                                                                                  >  1:2274837/1‑140 (MQ=255)                                                     gCCGGTACATTCCACTGTTCGTACCTGAATTAATTTATCGACCGGGATCCGTGATGGGCAATTTTTTGCGCATTTGCCACAGGCGATACAACTTTCGGCATTGCGACGAATCTTAAACGGCGACAATAGCGAAACCACGc                                                                               >  2:1740071/1‑140 (MQ=255)                                                         gTACATTCCACTGTTCGTACCTGAATTAATTTATCGACCGGGATCCGTGATGGGCAATTTTTTGCGCATTTGCCACAGGCGATACAACTTTCGGCATTGCGACGAATCTTAAACGGCGACAATAGCGAAACCACGCCCAt                                                                           >  2:2181737/1‑140 (MQ=255)                                                          tACATTCCACTGTTCGTACCTGAATTAATTTATCGACCGGGATCCGTGATGGGCAATTTTTTGCGCATTTGCCACAGGCGATACAACTTTCGGCATTGCGACGAATCTTAAACGGCGACAATAGCGAAACCACGCCCATc                                                                          <  2:2015033/140‑1 (MQ=255)                                                             aTTCCACTGTTCGTACCTGAATTAATTTATCGACCGGGATCCGTGATGGGCAATTTTTTGCGCATTTGCCACAGGCGATACAACTTTCGGCATTGCGACGAATCTTAAACGGCGACAATAGCGAAACCACGCCCATCAgc                                                                       <  2:2278924/140‑1 (MQ=255)                                                              ttCCACTGTTCGTACCTGAATTAATTTATCGACCGGGATCCGTGATGGGCAATTTTTTGCGCATTTGCCACAGGCGATACAACTTTCGGCATTGCGACGAATCTTAAACGGCGACAATAGCGAAACCACGCCCATCAgcg                                                                      <  2:2316098/140‑1 (MQ=255)                                                               tCCACTGTTCGTACCTGAATTAATTTATCGACCGGGATCCGTGATGGGCAATTTTTTGCGCATTTGCCACAGGCGATACAACTTTCGGCATTGCGACGAATCTTAAACGGCGACAATAGCGAAACCACGCCCATCAgcgc                                                                     >  1:2111778/1‑140 (MQ=255)                                                               tCCACTGTTCGTACCTGAATTAATTTATCGACCGGGATCCGTGATGGGCAATTTTTTGCGCATTTGCCACAGGCGATACAACTTTCGGCATTGCGACGAATCTTAAACGGCGACAATAGCGAAACCACGCCCATCAgcgc                                                                     <  2:2261183/140‑1 (MQ=255)                                                                ccACTGTTCGTACCTGAATTAATTTATCGACCGGGATCCGTGATGGGCAATTTTTTGCGCATTTGCCACAGGCGATACAACTTTCGGCATTGCGACGAATCTTAAACGGCGACAATAGCGAAACCACGCCCATCAgcgcg                                                                    <  2:2200537/140‑1 (MQ=255)                                                                    tGTTCGTACCTGAATTAATTTATCGACCGGGATCCGTGATGGGCAATTTTTTGCGCATTTGCCACAGGCGATACAACTTTCGGCATTGCGACGAATCTTAAACGGCGACAATAGCGAAACCACGCCCATCAGCGCGCCat                                                                >  1:1615875/1‑140 (MQ=255)                                                                     gTTCGTACCTGAATTAATTTATCGACCGGGATCCGTGATGGGCAATTTTTTGCGCATTTGCCACAGGCGATACAACTTTCGGCATTGCGACGAATCTTAAACGGCGACAATAGCGAAACCACGCCCATCAGCGCGCCata                                                               <  2:1918516/140‑1 (MQ=255)                                                                      ttCGTACCTGAATTAATTTATCGACCGGGATCCGTGATGGGCAATTTTTTGCGCATTTGCCACAGGCGATACAACTTTCGGCATTGCGACGAATCTTAAACGGCGACAATAGCGAAACCACGCCCATCAGCGCGCCatat                                                              >  1:1916382/1‑140 (MQ=255)                                                                      ttCGTACCTGAATTAATTTATCGACCGGGATCCGTGATGGGCAATTTTTTGCGCATTTGCCACAGGCGATACAACTTTCGGCATTGCGACGAATCTTAAACGGCGACAATAGCGAAACCACGCCCATCAGCGCGCCatat                                                              >  2:2096435/1‑140 (MQ=255)                                                                        cGTACCTGAATTAATTTATCGACCGGGATCCGTGATGGGCAATTTTTTGCGCATTTGCCACAGGCGATACAACTTTCGGCATTGCGACGAATCTTAAACGGCGACAATAGCGAAACCACGCCCATCAGCGCGCCATATgg                                                            >  1:1719536/1‑140 (MQ=255)                                                                         gTACCTGAATTAATTTATCGACCGGGATCCGTGATGGGCAATTTTTTGCGCATTTGCCACAGGCGATACAACTTTCGGCATTGCGACGAATCTTAAACGGCGACAATAGCGAAACCACGCCCATCAGCGCGCCATATggg                                                           >  2:1700906/1‑140 (MQ=255)                                                                             cTGAATTAATTTATCGACCGGGATCCGTGATGGGCAATTTTTTGCGCATTTGCCACAGGCGATACAACTTTCGGCATTGCGACGAATCTTAAACGGCGACAATAGCGAAACCACGCCCATCAGCGCGCCATATGGGCaaa                                                       >  2:2094017/1‑140 (MQ=255)                                                                                aattaattTATCGACCGGGATCCGTGATGGGCAATTTTTTGCGCATTTGCCACAGGCGATACAACTTTCGGCATTGCGACGAATCTTAAACGGCGACAATAGCGAAACCACGCCCATCAGCGCGCCATATGGGCAAAGAt                                                    >  1:2123757/1‑140 (MQ=255)                                                                                  ttaattTATCGACCGGGATCCGTGATGGGCAATTTTTTGCGCATTTGCCACAGGCGATACAACTTTCGGCATTGCGACGAATCTTAAACGGCGACAATAGCGAAACCACGCCCATCAGCGCGCCATATGGGCAAAGATaa                                                  <  1:1908614/140‑1 (MQ=255)                                                                                  ttaattTATCGACCGGGATCCGTGATGGGCAATTTTTTGCGCATTTGCCACAGGCGATACAACTTTCGGCATTGCGACGAATCTTAAACGGCGACAATAGCGAAACCACGCCCATCAGCGCGCCATATGGGCAAAGATaa                                                  <  1:1689176/140‑1 (MQ=255)                                                                                        tATCGACCGGGATCCGTGATGGGCAATTTTTTGCGCATTTGCCACAGGCGATACAACTTTCGGCATTGCGACGAATCTTAAACGGCGACAATAGCGAAACCACGCCCATCAGCGCGCCATATGGGCAAAGATAACGacac                                            <  2:2029539/140‑1 (MQ=255)                                                                                               cGGGATCCGTGATGGGCAATTTTTTGCGCATTTGCCACAGGCGATACAACTTTCGGCATTGCGACGAATCTTAAACGGCGACAATAGCGAAACCACGCCCATCAGCGCGCCATATGGGCAAAGATAACGACACCAGGCAt                                     <  1:2251068/140‑1 (MQ=255)                                                                                                 ggATCCGTGATGGGCAATTTTTTGCGCATTTGCCACAGGCGATACAACTTTCGGCATTGCGACGAATCTTAAACGGCGACAATAGCGAAACCACGCCCATCAGCGCGCCATATGGGCAAAGATAACGACACCAGGCATgg                                   >  1:2275150/1‑140 (MQ=255)                                                                                                      cGTGATGGGCAATTTTTTGCGCATTTGCCACAGGCGATACAACTTTCGGCATTGCGACGAATCTTAAACGGCGACAATAGCGAAACCACGCCCATCAGCGCGCCATATGGGCAAAGATAACGACACCAGGCATGGCGAAt                              <  1:1865018/140‑1 (MQ=255)                                                                                                        tGATGGGCAATTTTTTGCGCATTTGCCACAGGCGATACAACTTTCGGCATTGCGACGAATCTTAAACGGCGACAATAGCGAAACCACGCCCATCAGCGCGCCATATGGGCAAAGATAACGACACCAGGCATGGCGAATaa                            >  2:1823725/1‑140 (MQ=255)                                                                                                           tGGGCAATTTTTTGCGCATTTGCCACAGGCGATACAACTTTCGGCATTGCGACGAATCTTAAACGGCGACAATAGCGAAACCACGCCCATCAGCGCGCCATATGGGCAAAGATAACGACACCAGGCATGGCGAATAAACa                         <  1:1826531/140‑1 (MQ=255)                                                                                                            gggCAATTTTTTGCGCATTTGCCACAGGCGATACAACTTTCGGCATTGCGACGAATCTTAAACGGCGACAATAGCGAAACCACGCCCATCAGCGCGCCATATGGGCAAAGATAACGACACCAGGCATGGCGAATAAACAg                        >  2:1827516/1‑140 (MQ=255)                                                                                                            gggCAATTTTTTGCGCATTTGCCACAGGCGATACAACTTTCGGCATTGCGACGAATCTTAAACGGCGACAATAGCGAAACCACGCCCATCAGCGCGCCATATGGGCAAAGATAACGACACCAGGCATGGCGAATAAACAg                        >  1:2259552/1‑140 (MQ=255)                                                                                                                   tttttGCGCATTTGCCACAGGCGATACAACTTTCGGCATTGCGACGAATCTTAAACGGCGACAATAGCGAAACCACGCCCATCAGCGCGCCATATGGGCAAAGATAACGACACCAGGCATGGCGAATAAACAGGCTGGCa                 <  2:2274837/140‑1 (MQ=255)                                                                                                                        gcgcATTTGCCACAGGCGATACAACTTTCGGCATTGCGACGAATCTTAAACGGCGACAATAGCGAAACCACGCCCATCAGCGCGCCATATGGGCAAAGATAACGACACCAGGCATGGCGAATAAACAGGCTGGCAATCAg            <  2:2111778/140‑1 (MQ=255)                                                                                                                           cATTTGCCACAGGCGATACAACTTTCGGCATTGCGACGAATCTTAAACGGCGACAATAGCGAAACCACGCCCATCAGCGCGCCATATGGGCAAAGATAACGACACCAGGCATGGCGAATAAACAGGCTGGCAATCAGCaa         <  2:1899969/140‑1 (MQ=255)                                                                                                                                  cacaGGCGATACAACTTTCGGCATTGCGACGAATCTTAAACGGCGACAATAGCGAAACCACGCCCATCAGCGCGCCATATGGGCAAAGATAACGACACCAGGCATGGCGAATAAACAGGCTGGCAATCAGCAAAACGGTc  <  1:2154007/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                       GTGAAAAGGTCAATGTTGAGGCTACCGGACATGACTCTACGCAAGTCATACAGCCGGTACATTCCACTGTTCGTACCTGAATTAATTTATCGACCGGGATCCGTGATGGGCAATTTTTTGCGCATTTGCCACAGTCGATACAACTTTCGGCATTGCGACGAATCTTAAACGGCGACAATAGCGAAACCACGCCCATCAGCGCGCCATATGGGCAAAGATAACGACACCAGGCATGGCGAATAAACAGGCTGGCAATCAGCAAAACGGTC  >  NC_000913/1052471‑1052739

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑