Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,490,314	G→A	A546V (GCA→GTA)	oxc ←	oxalyl‑CoA decarboxylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,490,314	0	G	A	100.0%	128.7 / NA	38	A546V (GCA→GTA)	oxc	oxalyl‑CoA decarboxylase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (18/20);  total (18/20)

TTAGCATAATAGTATTTCCGATAAGGGTCCCCTCACCATCGCATAATGAGTTGGCAAGAGGAGACGATGTCAGTATTTAATTACCAGCGACTTGTTTTGGGTTAAGTTTGGTAATATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGAATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCAT  >  NC_000913/2490180‑2490446                                                                                                                                       |                                                                                                                                     ttAGCATAATAGTATTTCCGATAAGGGTCCCCTCACCATCGCATAATGAGTTGGCAAGAGGAGACGATGTCAGTATTTAATTACCAGCGACTTGTTTTGGGTTAAGTTTGGTAATATGGCCACTTTCAGTTCCTACTGCa                                                                                                                                 <  2:1620954/140‑1 (MQ=255) ttAGCATAATAGTATTTCCGATAAGGGTCCCCTCACCATCGCATAATGAGTTGGCAAGAGGAGACGATGTCAGTATTTAATTACCAGCGACTTGTTTTGGGTTAAGTTTGGTAATATGGCCACTTTCAGTTCCTACTGCa                                                                                                                                 <  1:2096885/140‑1 (MQ=255)            gTATTTCCGATAAGGGTCCCCTCACCATCGCATAATGAGTTGGCAAGAGGAGACGATGTCAGTATTTAATTACCAGCGACTTGTTTTGGGTTAAGTTTGGTAATATGGCCACTTTCAGTTCCTACTGCAGGGTCGATGAc                                                                                                                      <  2:1986412/140‑1 (MQ=255)            gTATTTCCGATAAGGGTCCCCTCACCATCGCATAATGAGTTGGCAAGAGGAGACGATGTCAGTATTTAATTACCAGCGACTTGTTTTGGGTTAAGTTTGGTAATATGGCCACTTTCAGTTCCTACTGCAGGGTCGATGAc                                                                                                                      <  1:2312679/140‑1 (MQ=255)             tATTTCCGATAAGGGTCCCCTCACCATCGCATAATGAGTTGGCAAGAGGAGACGATGTCAGTATTTAATTACCAGCGACTTGTTTTGGGTTAAGTTTGGTAATATGGCCACTTTCAGTTCCTACTGCAGGGTCGATGAcc                                                                                                                     >  2:1707494/1‑140 (MQ=255)                ttCCGATAAGGGTCCCCTCACCATCGCATAATGAGTTGGCAAGAGGAGACGATGTCAGTATTTAATTACCAGCGACTTGTTTTGGGTTAAGTTTGGTAATATGGCCACTTTCAGTTCCTACTGCAGGGTCGATGACcaca                                                                                                                  <  2:2285774/140‑1 (MQ=255)                  ccGATAAGGGTCCCCTCACCATCGCATAATGAGTTGGCAAGAGGAGACGATGTCAGTATTTAATTACCAGCGACTTGTTTTGGGTTAAGTTTGGTAATATGGCCACTTTCAGTTCCTACTGCa                                                                                                                                 <  2:1874002/123‑1 (MQ=255)                  ccGATAAGGGTCCCCTCACCATCGCATAATGAGTTGGCAAGAGGAGACGATGTCAGTATTTAATTACCAGCGACTTGTTTTGGGTTAAGTTTGGTAATATGGCCACTTTCAGTTCCTACTGCa                                                                                                                                 >  1:1874002/1‑123 (MQ=255)                     aTAAGGGTCCCCTCACCATCGCATAATGAGTTGGCAAGAGGAGACGATGTCAGTATTTAATTACCAGCGACTTGTTTTGGGTTAAGTTTGGTAATATGGCCACTTTCAGTTCCTACTGCAGGGTCGATGACCACATtaat                                                                                                             >  1:1727596/1‑140 (MQ=255)                     aTAAGGGTCCCCTCACCATCGCATAATGAGTTGGCAAGAGGAGACGATGTCAGTATTTAATTACCAGCGACTTGTTTTGGGTTAAGTTTGGTAATATGGCCACTTTCAGTTCCTACTGCAGGGTCGATGACCACATtaat                                                                                                             >  1:1918574/1‑140 (MQ=255)                              cccTCACCATCGCATAATGAGTTGGCAAGAGGAGACGATGTCAGTATTTAATTACCAGCGACTTGTTTTGGGTTAAGTTTGGTAATATGGCCACTTTCAGTTCCTACTGCAGGGTCGATGACCACATTAATAATGGTCgg                                                                                                    >  2:1672577/1‑140 (MQ=255)                                    ccATCGCATAATGAGTTGGCAAGAGGAGACGATGTCAGTATTTAATTACCAGCGACTTGTTTTGGGTTAAGTTTGGTAATATGGCCACTTTCAGTTCCTACTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTgcg                                                                                              <  2:1558454/140‑1 (MQ=255)                                    ccATCGCATAATGAGTTGGCAAGAGGAGACGATGTCAGTATTTAATTACCAGCGACTTGTTTTGGGTTAAGTTTGGTAATATGGCCACTTTCAGTTCCTACTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTgcg                                                                                              <  1:2161108/140‑1 (MQ=255)                                          cATAATGAGTTGGCAAGAGGAGACGATGTCAGTATTTAATTACCAGCGACTTGTTTTGGGTTAAGTTTGGTAATATGGCCACTTTCAGTTCCTACTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTg                                                                                        >  2:2307105/1‑140 (MQ=255)                                          cATAATGAGTTGGCAAGAGGAGACGATGTCAGTATTTAATTACCAGCGACTTGTTTTGGGTTAAGTTTGGTAATATGGCCACTTTCAGTTCCTACTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTg                                                                                        >  2:2097170/1‑140 (MQ=255)                                                     ggCAAGAGGAGACGATGTCAGTATTTAATTACCAGCGACTTGTTTTGGGTTAAGTTTGGTAATATGGCCACTTTCAGTTCCTACTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGAATACCggtgg                                                                             >  2:1744924/1‑140 (MQ=255)                                                     ggCAAGAGGAGACGATGTCAGTATATAATTACCAGCGACTTGTTTTGGGTTAAGTTTGGTAATATGGCCACTTTCAGTTCCTACTGCAGGGTCGATGACCACATTAATAA‑GGTCGGTTTGCGCGACTGAATACCggtggt                                                                            >  2:2125924/1‑140 (MQ=255)                                                       cAAGAGGAGACGATGTCAGTATTTAATTACCAGCGACTTGTTTTGGGTTAAGTTTGGTAATATGGCCACTTTCAGTTCCTACTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGAATACCGGTGGtt                                                                           >  1:1827294/1‑140 (MQ=255)                                                                aCGATGTCAGTATTTAATTACCAGCGACTTGTTTTGGGTTAAGTTTGGTAATATGGCCACTTTCAGTTCCTACTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGAATACCGGTGGTTAAAGCATGa                                                                  >  1:1871340/1‑140 (MQ=255)                                                                  gATGTCAGTATTTAATTACCAGCGACTTGGTTTGGGTTAAGTTTGGTAATATGGCCACTTTCAGTTCCTACTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGAATACCGGTGGTTAAAGCATGACg                                                                <  1:2043111/140‑1 (MQ=255)                                                                        aGTATTTAATTACCAGCGACTTGTTTTGGGTTAAGTTTGGTAATATGGCCACTTTCAGTTCCTACTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGAATACCGGTGGTTAAAGCATGACGAAGTTc                                                          >  2:1774729/1‑140 (MQ=255)                                                                        aGTATTTAATTACCAGCGACTTGTTTTGGGTTAAGTTTGGCAATATGGCCACTTTCAGTTCCTACTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGAATACCGGTGGTTAAAGCATGACGAAGTTc                                                          >  1:2175112/1‑140 (MQ=255)                                                                          tATTTAATTACCAGCGACTTGTTTTGGGTTAAGTTTGGTAATATGGCCACTTTCAGTTCCTACTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGAATACCGGTGGTTAAAGCATGACGAAGTTCAt                                                        <  2:1727596/140‑1 (MQ=255)                                                                            tttAATTACCAGCGACTTGTTTTGGGTTAAGTTTGGTAATATGGCCACTTTCAGTTCCTACTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGAATACCGGTGGTTAAAGCATGACGAAGTTCATCt                                                      >  2:1776939/1‑140 (MQ=255)                                                                             ttAATTACCAGCGACTTGTTTTGGGTTAAGTTTGGTAATATGGCCACTTTCAGTTCCTACTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGAATACCGGTGGTTAAAGCATGACGAAGTTCATCTg                                                     <  1:1707494/140‑1 (MQ=255)                                                                                       gCGACTTGTTTTGGGTTAAGTTTGGTAATATGGCCACTTTCAGTTCCTACTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGAATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGAc                                           >  1:1725260/1‑140 (MQ=255)                                                                                                  tGGGTTAAGTTTGGTAATATGGCCACTTTCAGTTCCTACTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGAATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCaa                                <  1:1748210/140‑1 (MQ=255)                                                                                                  tGGGTTAAGTTTGGTAATATGGCCACTTTCAGTTCCTACTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGAATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCaa                                <  1:1723835/140‑1 (MQ=255)                                                                                                       tAAGTTTGGTAATATGGCCACTTTCAGTTCCTACTGCAGGGTCGATGACCACATTAATAA‑GGTCGGTTTGCGCGACTGAATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCAc                          <  1:2125924/140‑1 (MQ=255)                                                                                                         aGTTTGGTAATATGGCCACTTTCAGTTCCTACTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGAATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACg                         >  2:1619062/1‑140 (MQ=255)                                                                                                          gTTTGGTAATATGGCCACTTTCAGTTCCTACTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGAATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGa                        <  2:1827294/140‑1 (MQ=255)                                                                                                               gTAATATGGCCACTTTCAGTTCCTACTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGAATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGc                   <  1:2225220/140‑1 (MQ=255)                                                                                                                            tttCAGTTCCTACTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGAATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTa           >  2:1598162/1‑135 (MQ=255)                                                                                                                            tttCAGTTCCTACTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGAATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTa           <  1:1598162/135‑1 (MQ=255)                                                                                                                            tttCAGTTCCTACTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGAATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAATTTg      >  2:2164167/1‑140 (MQ=255)                                                                                                                              tCAGTTCCTACTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGAATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAATTTGTc    <  1:2104237/140‑1 (MQ=255)                                                                                                                              tCAGTTCCTACTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGAATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAATTTGTc    <  2:1871340/140‑1 (MQ=255)                                                                                                                                aGTTCCTACTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGAATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCAt  <  1:1776939/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                     TTAGCATAATAGTATTTCCGATAAGGGTCCCCTCACCATCGCATAATGAGTTGGCAAGAGGAGACGATGTCAGTATTTAATTACCAGCGACTTGTTTTGGGTTAAGTTTGGTAATATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGAATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCAT  >  NC_000913/2490180‑2490446

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑