Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,494,424	T→C	intergenic (‑22/‑274)	yfdX ← / → ypdI	protein YfdX/colanic acid synthesis putative lipoprotein YpdI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,494,424	0	T	C	100.0%	132.3 / NA	37	intergenic (‑22/‑274)	yfdX/ypdI	protein YfdX/colanic acid synthesis putative lipoprotein YpdI
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (23/14);  total (23/14)

TGCTGAGTTTGCTGTTGTGCCGCTACCGGAGCATTATCAGCAGCCCATACAGTTGAAGATGCCAGAATTGCTGTGACCATCGTGGCCATAATTAAACGTTTCATACTTACCTCATTCTTTAACAGTTTTAGAAAACGCCGCCATCATATTGGGTGAGCATGAAATGAGAAATCAGAGAGCAACGGTCATTTTGAAAGGGTTATCTTACAGTTGTAGTTATTATCGCAAGAAGTATTTTAGTCTTACAATTGTAGGAATAT  >  NC_000913/2494299‑2494558                                                                                                                              |                                                                                                                                       tGCTGAGTTTGCTGTTGTGCCGCTACCGGAGCATTATCAGCAGCCCATACAGTTGAAGATGCCAGAATTGCTGTGACCATCGTGGCCATAATTAAACGTTTCATACTTACCTCATTCTTTAACAGCTTTAGAAAAcgccg                                                                                                                          <  1:2107210/140‑1 (MQ=255)   cTGAGTTTGCTGTTGTGCCGCTACCGGAGCATTATCAGCAGCCCATACAGTTGAAGATGCCAGAATTGCTGTGACCATCGTGGCCATAATTAAACGTTTCATACTTACCTCATTCTTTAACAGCTTTAGAAAACgccgcc                                                                                                                        <  1:2245140/140‑1 (MQ=255)   cTGAGTTTGCTGTTGTGCCGCTACCGGAGCATTATCAGCAGCCCATACAGTTGAAGATGCCAGAATTGCTGTGACCATCGTGGCCATAATTAAACGTTTCATACTTACCTCATTCTTTAACAGCTTTAGAAAACgccgcc                                                                                                                        <  1:1935053/140‑1 (MQ=255)           gCTGTTGTGCCGCTACCGGAGCATTATCAGCAGCCCATACAGTTGAAGATGCCAGAATTGCTGTGACCATCGTGGCCATAATTAAACGTTTCATACTTACCTCATTCTTTAACAGCTTTAGAAAACGCCGCCATCATAtt                                                                                                                >  2:1827060/1‑140 (MQ=255)                  tgCCGCTACCGGAGCATTATCAGCAGCCCATACAGTTGAAGATGCCAGAATTGCTGTGACCATCGTGGCCATAATTAAACGTTTCATACTTACCTCATTCTTTAACAGCTTTAGAAAACGCCGCCATCATATTGGGTGAg                                                                                                         >  2:1684488/1‑140 (MQ=255)                  tgCCGCTACCGGAGCATTATCAGCAGCCCATACAGTTGAAGATGCCAGAATTGCTGTGACCATCGTGGCCATAATTAAACGTTTCATACTTACCTCATTCTTTAACAGCTTTAGAAAACGCCGCCATCATATTGGGTGAg                                                                                                         >  2:2285234/1‑140 (MQ=255)                       cTACCGGAGCATTATCAGCAGCCCATACAGTTGAAGATGCCAGAATTGCTGTGACCATCGTGGCCATAATTAAACGTTTCATACTTACCTCATTCTTTAACAGCTTTAGAAAACGCCGCCATCATATTGGGTGAGCATGa                                                                                                    >  1:1770928/1‑140 (MQ=255)                           cGGAGCATTATCAGCAGCCCATACAGTTGAAGATGCCAGAATTGCTGTGACCATCGTGGCCATAATTAAACGTTTCATACTTACCTCATTCTTTAACAGCTTTAGAAAACGCCGCCATCATATTGGGTGAGCATGAAATg                                                                                                <  2:1922933/140‑1 (MQ=255)                                  ttATCAGCAGCCCATACAGTTGAAGATGCCAGAATTGCTGTGACCATCGTGGCCATAATTAAACGTTTCATACTTACCTCATTCTTTAACAGCTTTAGAAAACGCCGCCATCATATTGGGTGAGCATGAAATGAGAAATc                                                                                         >  2:1678621/1‑140 (MQ=255)                                    aTCAGCAGCCCATACAGTTGAAGATGCCAGAATTGCTGTGACCATCGTGGCCATAATTAAACGTTTCATACTTACCTCATTCTTTAACAGCTTTAGAAAACGCCGCCATCATATTGGGTGAGCATGAAATGAGAAATCag                                                                                       >  2:1872731/1‑140 (MQ=255)                                            cccATACAGTTGAAGATGCCAGAATTGCTGTGACCATCGTGGCCATAATTAAACGTTTCATACTTACCTCATTCTTTAACAGCTTTAGAAAACGCCGCCATCATATTGGGTGAGCATGAAATGAGAAATCAGAGAGCAAc                                                                               >  2:2230124/1‑140 (MQ=255)                                             ccATACAGTTGAAGATGCCAGAATTGCTGTGACCATCGTGGCCATAATTAAACGTTTCATACTTACCTCATTCTTTAACAGCTTTAGAAAACGCCGCCATCATATTGGGTGAGCATGAAATGAGAAATCAGAGAGCAACg                                                                              >  1:1847465/1‑140 (MQ=255)                                                tACAGTTGAAGATGCCAGAATTGCTGTGACCATCGTGGCCATAATTAAACGTTTCATACTTACCTCATTCTTTAACAGCTTTAGAAAACGCCGCCATCATATTGGGTGAGCATGAAATGAGAAATCAGAGAGCAACGGTc                                                                           >  1:1677996/1‑140 (MQ=255)                                                          gATGCCAGAATTGCTGTGACCATCGTGGCCATAATTAAACGTTTCATACTTACCTCATTCTTTAACAGCTTTAGAAAACGCCGCCATCATATTGGGTGAGCATGAAATGAGAAATCAGAGAGCAACGGTCATTTTGAAAg                                                                 >  1:2055542/1‑140 (MQ=255)                                                            tGCCAGAATTGCTGTGACCATCGTGGCCATAATTAAACGTTTCATACTTACCTCATTCTTTAACAGCTTTAGAAAACGCCGCCATCATATTGGGTGAGCATGAAATGAGAAATCAGAGAGCAACGGTCATTTTGAAAggg                                                               <  1:1918118/140‑1 (MQ=255)                                                               cAGAATTGCTGTGACCATCGTGGCCATAATTAAACGTTTCATACTTACCTCATTCTTTAACAGCTTTAGAAAACGCCGCCATCATATTGGGTGAGCATGAAATGAGAAATCAGAGAGCAACGGTCATTTTGAAAGGGTTa                                                            >  2:1591278/1‑140 (MQ=255)                                                                  aaTTGCTGTGACCATCGTGGCCATAATTAAACGTTTCATACTTACCTCATTCTTTAACAGCTTTAGAAAACGCCGCCATCATATTGGGTGAGCATGAAATGAGAAATCAGAGAGCAACGGTCATTTTGAAAGGGTTATCt                                                         <  1:1981925/140‑1 (MQ=255)                                                                    ttGCTGTGACCATCGTGGCCATAATTAAACGTTTCATACTTACCTCATTCTTTAACAGCTTTAGAAAACGCCGCCATCATATTGGGTGAGCATGAAATGAGAAATCAGAGAGCAACGGTCATTTTGAAAGGGTTATCTTa                                                       <  1:2040850/140‑1 (MQ=255)                                                                    ttGCTGTGACCATCGTGGCCATAATTAAACGTTTCATACTTACCTCATTCTTTAACAGCTTTAGAAAACGCCGCCATCATATTGGGTGAGCATGAAATGAGAAATCAGAGAGCAACGGTCATTTTGAAAGGGTTATCTTa                                                       <  1:1871913/140‑1 (MQ=255)                                                                       cTGTGACCATCGTGGCCATAATTAAACGTTTCATACTTACCTCATTCTTTAACAGCTTTAGAAAACGCCGCCATCATATTGGGTGAGCATGAAATGAGAAATCAGAGAGCAACGGTCATTTTGAAAGGGTTATCTTACAg                                                    <  1:1816357/140‑1 (MQ=255)                                                                       cTGTGACCATCGTGGCCATAATTAAACGTTTCATACTTACCTCATTCTTTAACAGCTTTAGAAAACGCCGCCATCATATTGGGAGAGCATGAAATGAGAAATCAGAGAGCAACGGGCATTTTGAAAGGGTTATCTTACAg                                                    >  2:1833462/1‑140 (MQ=255)                                                                           gACCATCGTGGCCATAATTAAACGTTTCATACTTACCTCATTCTTTAACAGCTTTAGAAAACGCCGCCATCATATTGGGTGAGCATGAAATGAGAAATCAGAGAGCAACGGTCATTTTGAAAGGGTTATCTTACAGTTGt                                                <  1:1760663/140‑1 (MQ=255)                                                                            aCCATCGTGGCCATAATTAAAAGTTTCATACTTACTTCATTCTTTAACAGCTTTAGAAAACGCCGCCATCATATTGGGTGAGCATGAAATTAGAAATCAGAGAGCAACGGGCATTTTGAAAGGGTTATCTTACAGTTGTa                                               >  2:1786385/1‑140 (MQ=255)                                                                                 cGTGGCCATAATTAAACGTTTCATACTTACCTCATTCTTTAACAGCTTTAGAAAACGCCGCCATCATATTGGGTGAGCATGAAATGAGAAATCAGAGAGCAACGGTCATTTTGAAAGGGTTATCTTACAGTTGTAGttat                                          >  2:1948301/1‑140 (MQ=255)                                                                                                 cGTTTCATACTTACCTCATTCTTTAACAGCTTTAGAAAACGCCGCCATCATATTGGGTGAGCATGAAATGAGAAATCAGAGAGCAACGGTCATTTTGAAAGGGTTATCTTACAGTTGTAGTTATTATCGCAAGAAGTAtt                          >  1:2328136/1‑140 (MQ=255)                                                                                                    ttCATACTTACCTCATTCTTTAACAGCTTTAGAAAACGCCGCCATCATATTGGGTGAGCATGAAATGAGAAATCAGAGAGCAACGGTCATTTTGAAAGGGTTATCTTACAGTTGTAGTTATTATCGCAAGAAGTATTTTa                       >  2:1653727/1‑140 (MQ=255)                                                                                                    ttCATACTTACCTCATTCTTTAACAGCTTTAGAAAACGCCGCCATCATATTGGGTGAGCATGAAATGAGAAATCAGAGAGCAACGGTCATTTTGAAAGGGTTATCTTACAGTTGTAGTTATTATCGCAAGAAGTATTTTa                       >  2:1853844/1‑140 (MQ=255)                                                                                                     tCATACTTACCTCATTCTTTAACAGCTTTAGAAAACGCCGCCATCATATTGGGTGAGCATGAAATGAGAAATCAGAGAGCAACGGTCATTTTGAAAGGGTTATCTTACAGTTGTAGTTATTATCGCAAGAAGTATTTTAg                      >  1:2043708/1‑140 (MQ=255)                                                                                                           ttACCTCATTCTTTAACAGCTTTAGAAAACGCCGCCATCATATTGGGTGAGCATGAAATGAGAAATCAGAGAGCAACGGTCATTTTGAAAGGGTTATCTTACAGTTGTAGTTATTATCGCAAGAAGTATTTTAGTCTTAc                <  1:1833462/140‑1 (MQ=255)                                                                                                             aCCTCATTCTTTAAGAGCTTTAGAAAACGCCGCCATCATATTGGGTGAGCATGAAATGAGAAATCAGAGAGCAACGGTCATTTTGAAAGGGTTATCTTACAGTTGTAGTTATTATCGCAAGAAGTATTTTAGTCTTACaa              >  2:1869518/1‑140 (MQ=255)                                                                                                             aCCTCATTCTTTAACAGCTTTAGAAAACGCCGCCATCATATTGGGTGAGCATGAAATGAGAAATCAGAGAGCAACGGTCATTTTGAAAGGGTTATCTTACAGTTGTAGTTATTATCGCAAGAAGTATTTTAGTCTTACaa              >  2:2320491/1‑140 (MQ=255)                                                                                                             aCCTCATTCTTTAACAGCTTTAGAAAACGCCGCCATCATATTGGGTGAGCATGAAATGAGAAATCAGAGAGCAACGGTCATTTTGAAAGGGTTATCTTACAGTTGTAGTTATTATCGCAAGAAGTATTTTAGTCTTACaa              <  1:1872731/140‑1 (MQ=255)                                                                                                                tCATTCTTTAACAGCTTTAGAAAACGCCGCCATCATATTGGGTGAGCATGAAATGAGAAATCAGAGAGCAACGGTCATTTTGAAAGGGTTATCTTACAGTTGTAGTTATTATCGCAAGAAGTATTTTAGTCTTACAATTg           <  1:2045619/140‑1 (MQ=255)                                                                                                                  aTTCTTTAACAGCTTTAGAAAACGCCGCCATCATATTGGGTGAGCATGAAATGAGAAATCAGAGAGCAACGGTCATTTTGAAAGGGTTATCTTACAGTTGTAGTTATTATCGCAAGAAGTATTTTAGTCTTACAATTGTa         >  1:2128912/1‑140 (MQ=255)                                                                                                                   ttCTTTAACAGCTTTAGAAAACGCCGCCATCATATTGGGTGAGCATGAAATGAGAAATCAGAGAGCAACGGTCATTTTGAAAGGGTTATCTTACAGTTGTAGTTATTATCGCAAGAAGTATTTTAGTCTTACAATTGTAg        >  2:1566192/1‑140 (MQ=255)                                                                                                                     cTTTAACAGCTTTAGAAAACGCCGCCATCATATTGGGTGAGCATGAAATGAGAAATCAGAGAGCAACGGTCATTTTGAAAGGGTTATCTTACAGTTGTAGTTATTATCGCAAGAAGTATTTTAGTCTTACAATTGTAGGa      <  1:2230124/140‑1 (MQ=255)                                                                                                                         aaCAGCTTTAGAAAACGCCGCCATCATATTGGGTGAGCATGAAATGAGAAATCAGAGAGCAACGGTCATTTTGAAAGGGTTATCTTACAGTTGTAGTTATTATCGCAAGAAGTATTTTAGTCTTACAATTGTAGGAatat  >  1:1931727/1‑140 (MQ=255)                                                                                                                              |                                                                                                                                       TGCTGAGTTTGCTGTTGTGCCGCTACCGGAGCATTATCAGCAGCCCATACAGTTGAAGATGCCAGAATTGCTGTGACCATCGTGGCCATAATTAAACGTTTCATACTTACCTCATTCTTTAACAGTTTTAGAAAACGCCGCCATCATATTGGGTGAGCATGAAATGAGAAATCAGAGAGCAACGGTCATTTTGAAAGGGTTATCTTACAGTTGTAGTTATTATCGCAAGAAGTATTTTAGTCTTACAATTGTAGGAATAT  >  NC_000913/2494299‑2494558

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑