Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,037,213	T→C	noncoding (73/77 nt)	ileT →	tRNA‑Ile

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,037,213	0	T	C	93.2%	238.1 / 4.8	74	noncoding (73/77 nt)	ileT	tRNA‑Ile
Reads supporting (aligned to +/- strand):  ref base T (5/0);  new base C (34/35);  total (39/35)
Fisher's exact test for biased strand distribution p-value = 5.59e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.47e-01

TAAAGAAGCGTTCTTTGAAGTGCTCACACAGATTGTCTGATGAAAATGAGCAGTAAAACCTCTACAGGCTTGTAGCTCAGGTGGTTAGAGCGCACCCCTGATAAGGGTGAGGTCGGTGGTTCAAGTCCACTCAGGCCTACCAAATTTGCACGGCAAATTTGAAGAGGTTTTAACTACATGTTATGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCTGCGGTTCGATCCCGCATAG  >  NC_000913/4037076‑4037327                                                                                                                                          |                                                                                                                   taAAGAAGCGTTCTTTGAAGTGCTCACACAGATTGTCTGATGAAAATGAGCAGTAAAACCTCTACAGGCTTGTAGCTCAGGTGGTTAGAGCGCACCCCTGATAAGGGTGAGGTCGGTGGTTCAAGTCCACTCAGGCCTAc                                                                                                                  >  2:2042390/1‑140 (MQ=34) taAAGAAGCGTTCTTTGAAGTGCTCACACAGATTGTCTGATGAAAATGAGCAGTAAAACCTCTACAGGCTTGTAGCTCAGGTGGTTAGAGCGCACCCCTGATAAGGGTGAGGTCGGTGGTTCAAGTCCACTCAGGCCTAc                                                                                                                  >  1:2165896/1‑140 (MQ=37)   aagaagCGTTCTTTGAAGTGCTCACACAGATTGTCTGATGAAAATGAGCAGTAAAACCTCTACAGGCTTGTAGCTCAGGTGGTTAGAGCGCACCCCTGATAAGGGTGAGGTCGGTGGTTCAAGTCCACTCAGGCCTACCa                                                                                                                >  2:2199478/1‑140 (MQ=35)         cGTTCTTTGAAGTGCTCACACAGATTGTCTGATGAAAATGAGCAGTAAAACCTCTACAGGCTTGTAGCTCAGGTGGTTAGAGCGCACCCCTGATAAGGGTGAGGTCGGTGGTTCAAGTCCACTCAGGCCTACCAAATTTg                                                                                                          >  2:1886616/1‑140 (MQ=34)         cGTTCTTTGAAGAGCTCACACAGATTGTCTGATGAAAATGAGCAGTAAAACCTCTACAGGCTTGTAGCTCAGGTGGTTAGAGCGCACCCCAGATAAGGGTGAGGTCGGTGGTTCAAGACCACAAAGGCCTACCAAATTTg                                                                                                          >  2:1668238/1‑140 (MQ=11)           ttCTTTGAAGTGCTCACACAGATTGTCTGATGAAAATGAGCAGTAAAACCTCTACAGGCTTGTAGCTCAGGTGGTTAGAGCGCACCCCTGATAAGGGTGAGGTCGGTGGTTCAAGTCCACTCAGGCCTACCAAATTTGCa                                                                                                        >  1:1750365/1‑140 (MQ=34)                                                                     cctttagctCAGCTGGTTAGAGCGCACCCCTGATAAGGGTGAGGTCGGTGGTTCGAGTCCACTAAGGCCCACCAttatcaacacatgagtgttgatcccattaaggggccatagctcagctgggagagcgcctgccttgc                                              <  1:1900064‑M2/136‑67 (MQ=255)                                                                      ctttagcTCAGCTGGTTAGAGAGCACCCCTGATAAGGGTGAGGTCGGTGGTTCGAGTCCACTAAGGCCCACCAttatcaacacatgagtgttgatcccattaaggggccatagctcagctgggagagcgcctgccttgca                                             >  2:373424‑M2/4‑73 (MQ=255)                                                                       tttagCTCAGCTGGTTAGAGCGCACCCCTGATAAGGGTGAGGTCGGTGGTTCGAGTCCACTAAGGCCCACCAttatcaacacatgagtgttgatcccattaaggggccatagctcagctgggagagcgcctgccttgcaa                                            <  2:1346434‑M2/138‑69 (MQ=255)                                                                        ttaGCTCAGCTGGTTAGAGCGCACCCCTGATAAGGGTGAGGTCGGTTGTTCGAGTCCACTAAGGCCCACCAttatcaacacatgagtgttgatcccaatacggggccatagctcagctgggagagcgcctgccttgcaag                                           >  2:1436364‑M2/2‑71 (MQ=255)                                                                        ttaGCTCAGCTGGTTAGAGCGCACCCCTGATAAGGGTGAGGTCGGTGGTTCGAGTCCACTAAGGCCCGCCAttatcaacacatgagtgttgatcccattaaggggccatagctcagctgggagagcgcctgccttgcaag                                           <  2:2169636‑M2/139‑70 (MQ=255)                                                                        ttaGCTCAGCTGGTTAGAGCGCACCCCTGATAAGGGTGAGGTCGGTGGTTCGAGTCCACTAAGGCCCACCAttatcaacacatgagtgttgatcccattaaggggccatagctcagctgggagagcgcctgccttgcaag                                           >  2:1121051‑M2/2‑71 (MQ=255)                                                                           gCTCAGCTGGTTAGAGCGCACCCCTGATAAGGGTGAGGTCGGTGGTTCGAGTCCACTAAGGCCCACCAttatcaacacatgagtgttgatcccaatacggggccatagctcagctgggagagcgcctgccttgcaagcag                                        >  2:249496‑M2/1‑68 (MQ=255)                                                                             tCAGCTGGTTAGAGCGCACCCCTGATAAGGGTGAGGTCGGTGGTTCTAGTCCACTAAGGCCCACCAttatcaacacatgagtgttgatcccaatacggggccatagctcagctgcgagagcgcctgccttgcaagcagga                                      >  1:1411760‑M2/1‑66 (MQ=255)                                                                             tCAGCTGGTTAGAGCGCACCCCTGATAAGGGTGAGGTCGGTGGTTCGAGTCCACTAAGGCCCACCAttatcaacacatgagtgttgatcccaatacggggccatagctcagctgggagagcgcctgccttgcaagcagga                                      >  2:19965‑M2/1‑66 (MQ=255)                                                                              cAGCTGGTTAGAGCGCACCCCTGATAAGGGTGAGGTCGGTGGTTCGAGTCCACTAAGGCCCACCAttatcaacacatgagtgttgatcccaatacggggccatagctcagctgggagagcgcctgccttgcaagcaggag                                     <  1:1294063‑M2/140‑76 (MQ=255)                                                                               agctggttagAGCGCACCCCTGATAAGGGTGAGGTCGGTGGTTCGAGTCCACTAAGGCCCACCAttatcaacacatgagtgttgatcccattaaggggccatagctcagctgggagagcgcctgccttgcaagcaggagg                                    <  2:1384288‑M2/137‑77 (MQ=255)                                                                                 ctggttAGAGCGCACCCCTGATAAGGGTGAGGTCGGTGGTTCGAGTCCACTAAGGCCCACCAttatcaacacatgagtgttgatcccaatacggggccatagctcagctgggagagcgcctgccttgcaagcaggaggtc                                  >  1:1451511‑M2/2‑62 (MQ=255)                                                                                  tggtTAGAGCGCACCCCTGATAAGGGTGAGGTCGGTGGTTCGAGTCCACTAAGGCCCACCAttatcaacacatgagtgttgatcccaatacggggccatagctcagctgggagagcgcctgccttgcaagcaggaggtca                                 >  2:2098445‑M2/1‑61 (MQ=255)                                                                                   ggtTAGAGCGCACCCCTGATAAGGGTGAGGTCGGTGGTTCGAGTCCACTAAGGCCCACCAttatcaacacatgagtgttgatcccattaaggggccatagctcagctgggagagcgcctgccttgcaagcaggaggtcag                                >  1:1372127‑M2/1‑60 (MQ=255)                                                                                   ggtTAGAGCGCACCCCTGATAAGGGTGAGGTCGGTGGTTCGAGTCCACTAAGGCCCACCAttatcaacacatgagtgttgatcccaatacggggccatagctcagctgggagagcgcctgccttgcaagcaggaggtcag                                >  1:255996‑M2/1‑60 (MQ=255)                                                                                   ggtTAGAGCGCACCCCTGATAAGGGTGAGGTCGGTGGTTCGAGTCCACTAAGGCCCACCAttatcaacacatgagtgttgatcccaatacggggccatagctcagctgggagagcgcctgccttgcaagcaggaggtcag                                >  2:55879‑M2/1‑60 (MQ=255)                                                                                   ggtTAGAGCGCACCCCTGATAAGGGTGAGGTCGGTGGTTCGAGTCCACTAAGGCCCACCAttatcaacacatgagtgttgatcccaatacggggccatagctcagctgggagagcgcctgccttgcaagcaggaggtcag                                >  1:726719‑M2/1‑60 (MQ=255)                                                                                   ggtTAGAGCGCACCCCTGATAAGGGTGAGGTCGGTGGTTCGAGTCCACTAAGGCCCACCAttatcaacacatgagtgttgatcccaatacggggccatagctcagctgggagagcgcctgccttgcaagcaggaggtcag                                >  1:815255‑M2/1‑60 (MQ=255)                                                                                         agCGCACCCCTGATAAGGGTGAGGTCGGTGGTTCGAGTCCACTAAGGCCCACCAttatcaacacatgagtgttgatcccattaaggggccatagctcagctgggagagcgcctgccttgcaagcaggaggtcagcggttc                          <  1:1376237‑M2/140‑87 (MQ=255)                                                                                           cgcACCCCTGATAAGGGTGAGGTCGGTGGTTCGAGTCCACTAAGGCCCACCAttatcaacacatgagtgtttatcccaatacggggccatagctcagctgggagagcgcctgccttgcaagcaggaggtcagcggttcga                        <  2:191391‑M2/140‑89 (MQ=255)                                                                                           cgcACCCCTGATAAGGGTGAGGTCGGTGGTTCGAGTCCACTAAGGCCCACCAttatcaacacatgagtgttgatcccaatacggggccatagctcagctgggatagcgcctgccttgcaagcaggaggtcagcggttaga                        <  2:1186710‑M2/140‑89 (MQ=255)                                                                                           cgcACCCCTGATAAGGGTGAGGTCGGTGGTTCGAGTCCACTAAGGCCCACCAttatcaacacatgagtgttgatcccaatacggggccatagctcagctgggagagcgcctgccttgcaagcaggaggtcagcggttcga                        <  1:213827‑M2/140‑89 (MQ=255)                                                                                           cgcACCCATGATAAGGGTGAGGTCGGTGGTTCGAGTCCACTAAGGCCCACCAttatcaacacatgagtgttgatcccattaaggggccatagctcagctgggagagcgcctgccttgcaagcaggaggtcagcggttcga                        <  1:881194‑M2/140‑89 (MQ=255)                                                                                            gcACCCCTGATAAGGGTGAGGTCGGTGGTTCGAGTCCACTAAGGCCCACCAttatcaacacatgagtgttgatcccattaaggggccatagctcagctgggagagcgcctgccttgcaagcaggaggtcagcggttcgat                       >  2:435597‑M2/1‑51 (MQ=255)                                                                                             cACCCCTGATAAGGGTGAGGTCGGTGGTTCGAGTCCACTAAGGCCCACCAttatcaacacatgagtgttgatcccaatacggggccatagctcagctgggagagcgcctgccttgcaagcaggaggtcagcggttcgatc                      >  2:1294063‑M2/1‑50 (MQ=255)                                                                                             cACCCCTGATAAGGGTGAGGTCGGTGGTTCGAGTCCACTAAGGCCCACCAttatcaacacatgagtgttgatcccaatacggggccatagctcagctgggagagcgcctgccttgcaagcaggaggtcagcggttcgatc                      >  1:384781‑M2/1‑50 (MQ=255)                                                                                              aCCCCTGATAAGGGTGAGGTCGGTGGTTCGAGTCCACTAAGGCCCACCAttatcaacacatgagtgttgatcccaatacggggccatagctcagctgggagagcgcctgccttgcaagcaggaggtcag                                >  1:1171190‑M2/1‑49 (MQ=255)                                                                                              aCCCCTGATAAGGGTGAGGTCGGTGGTTCGAGTCCACTAAGGCCCACCAttatcaacacatgagtgttgatcccaatacggggccatagctcagctgggagagcgcctgccttgcaagcaggaggtcag                                <  2:1171190‑M2/129‑81 (MQ=255)                                                                                                cccTGATAAGGGTGAGGTCGGTGGTTCGAGTCCACTAAGGCCCACCAttatcaacacatgagtgttgatcccaatacggggccatagctcagctgggatagcgcctgccttgcaagcaggaggtcagcggttagatcccg                   >  1:1186710‑M2/1‑47 (MQ=255)                                                                                                cccTGATAAGGGTGAGGTCGGTGGTTCGAGTCCACTAAGGCCCACCAttatcaacacatgagtgttgatcccaatacggggccatagctcagctgggagagcgcctgccttgcaagcaggaggtcagcggttcgatcccg                   <  1:983467‑M2/140‑94 (MQ=255)                                                                                                cccTGATAAGGGTGAGGTAGGTGGTTCGAGTCCACTAAGGCCCACCAttatcaacacatgagtgttgatcccaatacggggccatagctcagctgggagagcgcctgccttgcaagcaggaggtcagcggttcgatcccg                   <  1:531534‑M2/140‑94 (MQ=255)                                                                                                 ccTGATAAGGGTGAGGTCGGTGGTTCGAGTCCACTAAGGCCCACCAttatcaacacatgagtgttgatcccattaaggggccatagctcagctgggagagcgcctgcattgcaagcaggaggtcagcggttcgatcccgc                  >  1:673176‑M2/1‑46 (MQ=255)                                                                                                 ccTGATAAGGGTGAGGTCGGTGGTTCGAGTCCACTAAGGCCCACCAttatcaacacatgagtgttgatcccaatacggggccatagctcagctgggagagcgcctgccttgcaagcaggaggtcagcggttcgatcccgc                  >  2:983467‑M2/1‑46 (MQ=255)                                                                                                   tGATAAGGGTGAGGTCGGTGGTTCGAGTCCACTAAGGCCCACCAttatcaacacatgagtgttgatcccaatacggggccatagctcagctgggagagcgcctgccttgcaagcaggaggtcagtggttcgatcccgctt                >  1:888155‑M2/1‑44 (MQ=255)                                                                                                   tGATAAGGGTGAGGTCGGTGGTTCGAGTCCACTAAGGCCCACCAttatcaacacatgagtgttgatcccaatacggggccatagctcagctgggagagcgcctgccttgcaagcaggaggtcagcggttcgatcccgctt                >  1:235358‑M2/1‑44 (MQ=255)                                                                                                   tGATAAGGGTGAGGTCGGTGGTTCGAGTCCACTAAGGCCCACCAttatcaacacatgagtgttgatcccaatacggggccatagctcagctgggagagcgcctgccttgcaagcaggaggtcagcggttcgatcccgctt                >  2:104934‑M2/1‑44 (MQ=255)                                                                                                   tGATAAGGGTGAGGTCGGTGGTTCGAGTCCACTAAGGCCCACCAttatcaacacatgagtgttgatcccaatacggggccatagctcagctgggagagcgcctgccttgcaagcaggaggtaagcggttcgatcccgctt                >  2:704249‑M2/1‑44 (MQ=255)                                                                                                     aTAAGGGTGAGGTCGGTGGTTCGAGTCCACTAAGGCCCACCAttatcaacacatgagtgttgatcccattaaggggccatagctcagctgggagagcgcctgccttgcaagcaggaggtcagcggttcgatcccgcttgg              <  1:540404‑M2/140‑99 (MQ=255)                                                                                                     aTAAGGGTGAGGTCGGTGGTTCGAGTCCACTAAGGCCCACCAttatcaacacatgagtgttgatcccaatacggggccatagctcagctgggagagcgcctgccttgcaagcaggaggtcagcggttcgatcccgcttgg              <  2:77029‑M2/140‑99 (MQ=255)                                                                                                      tAATGGTGAGGTCGGTGGTTCGAGTCCACTAAGGCCCACCAttatcaacacatgagtgttgatcccattaaggggccatagctcagctgggagagcgcctgccttgcaagcaggatgtcagcggttcgatcccgcttggc             >  2:396223‑M2/1‑41 (MQ=255)                                                                                                      tAAGGGTGAGGTCGGTGGTTCGAGTCCACTAAGGCCCACCAttatcaacacatgagtgttgatcccattaaggggccatagctcagctgggagagcgcctgccttgcaagcaggaggtcagcggttcgatcccgcttggc             >  2:110482‑M2/1‑41 (MQ=255)                                                                                                          ggTTAGGTCGGTGGTTCGAGTCCACTAAGGCCCACCAttatcaacacatgagtgttgatcccaatacggggccatagctcagctgggagagcgcctgccttgcaagcaggatgtcagcggttcgatcccgcttggctcca         <  2:1056233‑M2/140‑104 (MQ=255)                                                                                                          ggTGAGGTCGGTGGTTCGAGTCCACTAAGGCCCACCAttatcaacacatgagtgttgatcccattaaggggccatagctcagctgggatagcgcctgccttgcaagcaggaggtcagcggttcgatcccgcttggctcca         <  2:922173‑M2/140‑104 (MQ=255)                                                                                                          ggTGAGGTCGGTGGTTCGAGTCCACTAAGGCCCACCAttatcaacacatgagtgttgatcccattaaggggccatagctcagctgggagagcgcctgccttgcaagcaggaggtcagcggttcgatcccgcttggctcca         <  1:524313‑M2/140‑104 (MQ=255)                                                                                                          ggTGAGGTCGGTGGTTCGAGTCCACTAAGGCCCACCAttatcaacacatgagtgttgatcccattaaggggccatagctcagctgggagagcgcctgccttgcaagcaggaggtcagcggttcgatcc                     <  1:533366‑M2/128‑92 (MQ=255)                                                                                                          ggTGAGGTCGGTGGTTCGAGTCCACTAAGGCCCACCAttatcaacacatgagtgttgatcccattaaggggccatagctcagctgggagagcgcctgccttgcaagcaggaggtcagcggttcgatcc                     >  2:533366‑M2/1‑37 (MQ=255)                                                                                                          ggTGAGGTCGGTGGTTCGAGTCCACTAAGGCCCACCAttatcaacacatgagtgttgatcccaatacggggccatagctcagctgggagagcgcctgccttgcaagcaggaggtcagcggttcgatcccgcttggctcca         <  2:725310‑M2/140‑104 (MQ=255)                                                                                                          ggTGAGGTCGGTGGTTCGAGTCCACTAAGGCCCACCAttatcaacacatgagtgttgatcccaatacggggccatagctcagctgggagagcgcctgccttgcaagcaggaggtcagcggttcgatcccgcttggctcca         <  2:1209749‑M2/140‑104 (MQ=255)                                                                                                          ggTGAGGTCGGTGGTTCGAGTCCACTAAGGCCCACCAttatcaacacatgagtgttgatcccaatacggggccatagctcagctgggagagcgcctgccttgcaagcaggaggtcagcggttcgatcccgcttggctcca         <  2:529010‑M2/140‑104 (MQ=255)                                                                                                          ggTGAGGTCGGTGGTTCGAGTCCACTAAGGCCCACCAttatcaacacatgagtgttgatcccaatacggggccatagctcagctgggagagcgcctgccttgcaagcaggaggtcagcggttcgatcccgcttggctcca         <  1:101290‑M2/140‑104 (MQ=255)                                                                                                          ggTGAGGTCGGTGGTTCGAGTCCACTAAGGCCCACCAttatcaacacatgagtgttgatcccaatacggggccatagctcagctgggagagcgcctgccttgcaagcaggaggtcagcggttcgatcccgcttggctcca         <  1:1235344‑M2/140‑104 (MQ=255)                                                                                                          ggTGAGGTCGGTGGTTCGAGTCCACTAAGGCCCACCAttatcaacacatgagtgttgatcccaatacggggccatagctcagctgggagagcgcctgccttgcaagcaggaggtcagcggttcgatcccgcttggc             >  1:202062‑M2/1‑37 (MQ=255)                                                                                                          ggTGAGGTCGGTGGTTCGAGTCCACTAAGGCCCACCAttatcaacacatgagtgttgatcccaatacggggccatagctcagctgggagagcgcctgccttgcaagcaggaggtcagcggttcgatcccgcttggc             <  2:202062‑M2/136‑100 (MQ=255)                                                                                                            tGAGGTCGGTGGTTCGAGTCCACTAAGGCCCACCAttatcaacacatgagtgttgatcccattaaggggccatagctcagctgggagagcgcctgccttgcaagcaggaggtcagcggttcgatcccgcttggctccatt       <  1:1444867‑M2/140‑106 (MQ=255)                                                                                                            tGAGGTCGGTGGTTCGAGTCCACTAAGGCCCACCAttatcaacacatgagtgttgatcccattaaggggccatagctcagctgggagagcgcctgccttgcaagcaggaggtcagcggttcgatcccgcttggctccatt       <  2:283561‑M2/140‑106 (MQ=255)                                                                                                            tGAGGTCGGTGGTTCGAGTCCACTAAGGCCCACCAttatcaacacatgagtgttgatcccattaaggggccatagctcagctgggagagcgcctgccttgcaagcaggaggtcagcggttcgatcccgcttggctccatt       <  2:508768‑M2/140‑106 (MQ=255)                                                                                                            tGAGGTCGGTGGTTCGAGTCCACTAAGGCCCACCAttatcaacacatgagtgttgatcccattaaggggccatagctcagctgggagagcgcctgccttgcaagcaggaggtcagcggttagatcccgcttggctccatt       <  2:36239‑M2/140‑106 (MQ=255)                                                                                                            tGAGGTCGGTGGTTCGAGTCCACTAAGGCCCACCAttatcaacacatgagtgttgatcccaatacggggccatagctcagctgggagagcgcctgccttgcaagcaggaggtcagcggttcgatcccgcttggctccatt       <  1:243179‑M2/140‑106 (MQ=255)                                                                                                             gAGGTCGGTGGTTCGAGTCCACTAAGGCCCACCAttatcaacacatgagtgttgatcccaatacggggccatagctcagctgggagagcgcctgccttgcaagcaggaggtcagcggttcgatc                      <  2:50965‑M2/124‑91 (MQ=255)                                                                                                             gAGGTCGGTGGTTCGAGTCCACTAAGGCCCACCAttatcaacacatgagtgttgatcccaatacggggccatagctcagctgggagagcgcctgccttgcaagcaggaggtcagcggttcgatc                      >  1:50965‑M2/1‑34 (MQ=255)                                                                                                              aGGTCGGTGGTTCGAGTCCACTAAGGCCCACCAttatcaacacatgagtgttgatcccattaaggggccatagctcagctgggagagcgcctgccttgcaagcaggaggtcagcggttcgatcccgcttggctccatttg     <  2:1549939‑M2/140‑108 (MQ=255)                                                                                                              aGGTCGGTGGTTCGAGTCCACTAAGGCCCACCAttatcaacacatgagtgttgatcccaataagggccatagctcagctgggagagcgcctgccttgcaagcaggaggtcagcggttcgatcccgcttggctccatttgt     <  2:1147873‑M2/140‑108 (MQ=255)                                                                                                               ggTCGGTGGTTCGAGTCCACTAAGGCCCACCAttatcaacacatgagtgttgatcccattaaggggccatagctcagctgggagagcgcctgccttgcaagcaggaggtcagcggttcgatcccgcttggctccatttgt    <  2:1114860‑M2/140‑109 (MQ=255)                                                                                                               ggTCGGTGGTTCGAGTCCACTAAGGCCCACCAttatcaacacatgagtgttgatcccaatacggggccatagctcagctgggagagcgcctgccttgcaagcaggaggtcagcggttcgatcccgcttggctccatttgt    >  2:436110‑M2/1‑32 (MQ=255)                                                                                                               ggTCGGTGGTTCGAGTCCACTAAGGCCCACCAttatcaacacatgagtgttgatcccaatacggggccatagctcagctgggagagcgcctgccttgcaagcaggaggtcagcggttcgatcccgcttggctccatttgt    >  2:243179‑M2/1‑32 (MQ=255)                                                                                                               ggTCGGTGGTTCGAGTCCACTAAGGCCCACCAttatcaacacatgagtgttgatcccaatacggggccatagctcagctgggagagcgcctgccttgcaagcaggaggtcagcggttcgatcccgcttggctccatttgt    >  2:101290‑M2/1‑32 (MQ=255)                                                                                                                 tCGGTGGTTCGAGTCCACTAAGGCCCACCAttatcaacacatgagtgttgatcccattaaggggccatagctcagctgggagagcgcctgccttgcaagcaggaggtcagcggttcgatcccgcttggctccatttgttc  >  1:1159044‑M2/1‑30 (MQ=255)                                                                                                                    gtggtTCGAGTCCACTAAGGCCCACCAttatcaacacatgagtgttgatcccaatacggggccatagctcagctgggagagcgcctgccttgcaagcaggaggtcagcggttcgatcccgctt                >  1:948940‑M2/1‑27 (MQ=255)                                                                                                                    gtggtTCGAGTCCACTAAGGCCCACCAttatcaacacatgagtgttgatcccaatacggggccatagctcagctgggagagcgcctgccttgcaagcaggaggtcagcggttcgatcccgctt                <  2:948940‑M2/123‑97 (MQ=255)                                                                                                                                          |                                                                                                                   TAAAGAAGCGTTCTTTGAAGTGCTCACACAGATTGTCTGATGAAAATGAGCAGTAAAACCTCTACAGGCTTGTAGCTCAGGTGGTTAGAGCGCACCCCTGATAAGGGTGAGGTCGGTGGTTCAAGTCCACTCAGGCCTACCAAATTTGCACGGCAAATTTGAAGAGGTTTTAACTACATGTTATGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCTGCGGTTCGATCCCGCATAG  >  NC_000913/4037076‑4037327

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑