BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	224,906	C→T	noncoding (1136/1542 nt)	rrsH →	16S ribosomal RNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	224,906	0	C	T	88.7%	79.1 / 117.8	438	noncoding (1136/1542 nt)	rrsH	16S ribosomal RNA
Reads supporting (aligned to +/- strand): ref base C (19/20); major base T (155/199); minor base A (19/26); total (193/245)
Fisher's exact test for biased strand distribution p-value = 8.75e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.12e-01

Only 100 of 441 total aligned reads displayed.

CCACAGAACTTTCCAGAGATGGATTGGTGCCTTCGGGAACTGTGAGACAGGTGCTGCATGGCTGTCGTCAGCTCGTGTTGTGAAATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATCTTTTGTTGCCAGC‑GGTCCGGCCGGGAACTCAAAGGAGACTGCCAGTGATAAACTGGAGGAAGGTGGGGATGACGTCAAGTCATCATGGCCCTTACGACCAGGGCTACACACGTGCTACAATGGCGCATACAAAGAGAAG  >  NC_000913/224768‑225028

ccACAGAACTTTCCAGAGATGGATTGGTGCCTTCGGGAACTGTGAGACAGGTGCTGCATGGCTGTCGTCAGCTCGTGTTGTGAAATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATCTTTTGTTGCCAGC‑GGTcc                                                                                                                           >  1:1691235/1‑140 (MQ=255)

                             ccTTCGGGAACTGTGAGACAGGTGCTGCATGGCTGTCGTCAGCTCGTGTTGTGAAATGTTGGGTTAAGTCCCGCAACGAGCGGAACCCTTATCTTTTGTTGCCAGC‑GGTCCGGCCGGGAACTCAAAGGAGACTGCCAGTg                                                                                              <  2:1611368/140‑1 (MQ=21)

                                        tgtgAGTCAGGTGCTGCATGGCTGTCGTCAGCTCGTGTTGTGAAATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATCTTTTGTTGCCAGC‑GGTCCGGCCGGGAACTCAAAGGAGACTGCCAGTGATAAACTggag                                                                                   <  2:2116611/140‑1 (MQ=18)

                                         tcaagaCAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCtgagg                                                                                  <  1:1447468/137‑1 (MQ=2)

                                          caagaCAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCggagga                                                                                 <  2:1894016/138‑1 (MQ=2)

                                              aCAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGt                                                                             <  1:755505/140‑1 (MQ=2)

                                               cAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCTCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTg                                                                            <  2:1428604/140‑1 (MQ=2)

                                                 ggtggtGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTggg                                                                          >  1:1253238/1‑140 (MQ=2)

                                                  gtggtgcATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAAc                                                                                        >  2:1210536/5‑125 (MQ=2)

                                                  gtggtgcATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTgggg                                                                         >  1:539176/5‑140 (MQ=2)

                                                  gtggtgCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGCACGTAAGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTggg                                                                          >  2:1539596/1‑140 (MQ=2)

                                                  gtggtgCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTTGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTgggg                                                                         >  2:1315409/1‑140 (MQ=2)

                                                  gtggtgCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCggggg                                                                                  >  1:446709/1‑131 (MQ=2)

                                                  gtggtgCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTgggg                                                                         <  1:2279759/140‑1 (MQ=2)

                                                  gtggtgCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTgggg                                                                         <  2:1026342/140‑1 (MQ=2)

                                                  gtggtgCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAAAGAGACTGCCGGTGACaaa                                                                                         <  2:1191616/124‑1 (MQ=2)

                                                  gtggtgCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAAc                                                                                        <  1:1210536/125‑1 (MQ=2)

                                                   tggtgcATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCAGCAACGAGCGCAACCCTTATTTTTAGTTGCCAGC‑ACTTTGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGa                                                                        >  1:1421928/4‑140 (MQ=2)

                                                    ggtgcATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTTGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGAt                                                                       <  1:909270/138‑1 (MQ=2)

                                                    ggtgcATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGAt                                                                       <  2:451761/138‑1 (MQ=2)

                                                       gcATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGAc                                                                    >  2:673291/1‑140 (MQ=11)

                                                       gcATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTCAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGAc                                                                    >  1:71082/1‑140 (MQ=2)

                                                        cATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTTGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCggagga                                                                                 <  1:992973/126‑1 (MQ=2)

                                                          tGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTTGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCggagg                                                                                  >  1:337969/1‑123 (MQ=2)

                                                            gttGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCaa                                                               <  2:616548/138‑1 (MQ=2)

                                                            gttGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCaa                                                               >  2:232811/3‑140 (MQ=2)

                                                            gttGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAGAAAGACTGCAGGTGACAAACCGGAGGAAGGTGGGGATGACGTCaa                                                               >  1:1312796/3‑140 (MQ=2)

                                                            gttGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCaa                                                               >  1:2023120/3‑140 (MQ=11)

                                                            gttGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCaa                                                               >  1:1800566/3‑140 (MQ=11)

                                                            gttGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCATATTTTTAGTTGCCAGC‑ACTTTGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACg                                                                   <  2:425964/134‑1 (MQ=2)

                                                             ttGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGCACGTAAGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCaa                                                               >  2:929554/2‑140 (MQ=2)

                                                             ttGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGAt                                                                       <  1:1066580/130‑1 (MQ=2)

                                                             ttGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCaaa                                                              <  2:1516011/139‑2 (MQ=2)

                                                             ttGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAATTGCCAGC‑ACTTCGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCaaa                                                              <  2:1485163/139‑2 (MQ=2)

                                                              tGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGCACGTAAGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCaaa                                                              <  1:2079/140‑2 (MQ=2)

                                                                tcgtcAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAAtca                                                           >  2:78836/1‑140 (MQ=11)

                                                                     aGCTCGTGTCGTTAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATg                                                      <  1:583765/140‑1 (MQ=2)

                                                                     aGCTCGTGTCGTGAGATGTTTGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAatcatc                                                         <  2:1092613/137‑1 (MQ=2)

                                                                     aGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTTGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATg                                                      <  2:2273377/140‑1 (MQ=2)

                                                                     aGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGTGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATg                                                      <  1:1001251/140‑1 (MQ=2)

                                                                     aGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATg                                                      <  2:573227/140‑1 (MQ=2)

                                                                      gCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACTAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATgc                                                     <  1:304893/140‑2 (MQ=2)

                                                                      gCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGCACGTAAGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGt                                                                  <  1:489725/128‑1 (MQ=2)

                                                                      gCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTTGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATgc                                                     <  1:153237/140‑2 (MQ=2)

                                                                      gCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTTGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATgc                                                     <  2:1820196/140‑2 (MQ=2)

                                                                      gCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTTGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATgc                                                     <  2:411908/140‑2 (MQ=2)

                                                                      gCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTATAAAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATgc                                                     <  2:285398/140‑2 (MQ=2)

                                                                      gCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATgc                                                     <  2:1345317/140‑2 (MQ=2)

                                                                       cTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTTGGGTGGGCACTCTAAAGAGACTGCCGGTTACAAACCGGAGGAAGGTGGGGATGACGTCaaa                                                              >  1:1354586/1‑129 (MQ=2)

                                                                       cTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTTGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATgcc                                                    >  1:104563/1‑138 (MQ=2)

                                                                          gtgtCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCt                                                 >  2:308122/1‑140 (MQ=2)

                                                                           tgtcgtGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCtt                                                <  2:549385/140‑1 (MQ=2)

                                                                           tgtagtGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCtt                                                <  2:830041/140‑1 (MQ=2)

                                                                            gtcgtgAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTTGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATg                                                      >  2:1260410/4‑133 (MQ=2)

                                                                            gtcgtgAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTa                                               <  2:966293/137‑1 (MQ=2)

                                                                               gtgAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGCACGTAAGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTatg                                             >  1:117973/1‑138 (MQ=2)

                                                                                tgAAATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATCTTTTGTTGCCAGC‑GGTCCGGCCGGGAACTCAAAGGAGACTGCCAGTGATAAACTGGAGGAAGGTGGGGATGACGTCAAGTCATCATGGCCCTTACGAc                                           <  1:1620517/140‑1 (MQ=32)

                                                                                tgAAATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATCTTTTGTTGCCAGC‑GGTCCGGCCGGGAACTCAAAGGAGACTGCCAGTGATAAACTGGAGGAAGGTGGGGATGACGTCAAGTCATCATGGCCCTTACGAc                                           <  2:1691235/140‑1 (MQ=32)

                                                                                 gagaTGTTGTGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGAcc                                          <  2:1042478/137‑1 (MQ=2)

                                                                                 gagaTGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGAcc                                          <  2:1411019/137‑1 (MQ=2)

                                                                                  agaTGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTTGGGTGGACACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGAcct                                         <  2:1049863/138‑2 (MQ=2)

                                                                                     tGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTTGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCAT‑GCCCTTATGACCTGGGc                                     <  1:53007/140‑1 (MQ=2)

                                                                                      gTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTAAAATCATCATGCCCCTTATGAcct                                         <  2:1301372/136‑2 (MQ=2)

                                                                                      gTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTAAAATCATCATGCCCCTTATGAcct                                         >  1:1301372/1‑135 (MQ=2)

                                                                                      gTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTAT‑TTTAGTTGCCAGC‑ACTTTGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGCt                                    >  2:585829/1‑140 (MQ=2)

                                                                                        tGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTTGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGCTa                                   >  1:387294/1‑140 (MQ=2)

                                                                                         gggTTAAGTCCCGCAACGAGCGCAACCCTTATCTTTTGTTGCCAGC‑GGTCCGGCCGGGAACTCAAAGGAGACTGCCAGTGATAAACTGGAGGAAGGTGGGGATGACGTCAAGTCATCATGGCCCTTACGACCAGGGCTac                                  <  1:1737023/140‑1 (MQ=32)

                                                                                         gggTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGCTac                                  <  1:928143/140‑1 (MQ=2)

                                                                                         gggTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGCTac                                  >  2:651683/1‑140 (MQ=2)

                                                                                          ggTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGCTa                                   <  2:523957/138‑1 (MQ=2)

                                                                                          ggTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAGAAAGACTACCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGAcc                                          >  2:1178933/1‑131 (MQ=2)

                                                                                           gTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTTGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGCTacac                                <  1:810585/140‑1 (MQ=2)

                                                                                           gTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTTGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGCTacac                                <  1:1232537/140‑1 (MQ=2)

                                                                                           gTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGCTacac                                <  1:115504/140‑1 (MQ=2)

                                                                                           gTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGCTacac                                <  1:2125809/140‑1 (MQ=2)

                                                                                           gTTAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGCTacac                                <  1:626750/140‑1 (MQ=2)

                                                                                            ttAAGTCCCGCAACGAGCGCAACCCTTATCTTTTGTTGCCAGC‑GGTCCGGCCGGGAACTCAAAGGAGACTGCCAGTGATAAACTGGAGGAAGGTGGGGATGACGTCAAGTCATCATGGCCCTTACGACCAGGGCTacaca                               >  1:2212793/1‑140 (MQ=33)

                                                                                            ttAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGCACGTAAGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGCTacac                                <  2:748102/140‑1 (MQ=2)

                                                                                            ttAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGCTacaca                               <  1:145435/140‑1 (MQ=2)

                                                                                             tAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGCACGTAAGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGCTacaca                               <  1:147754/140‑1 (MQ=2)

                                                                                             tAAGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGCTacacac                              >  1:895913/1‑140 (MQ=2)

                                                                                              aaGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTTGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGCTACACACg                             <  2:387294/140‑1 (MQ=2)

                                                                                               aGTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGCTACACACg                             <  2:1120377/139‑1 (MQ=2)

                                                                                                gTCCCGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGCTACACACGta                           <  2:895913/140‑2 (MQ=2)

                                                                                                gTCACGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGCTACACACGta                           <  1:97809/140‑2 (MQ=2)

                                                                                                    cGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGCTacac                                <  2:1452463/131‑1 (MQ=2)

                                                                                                    cGCAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATAATCATGCCCCTTATGACCTGGGc                                     >  2:874805/1‑126 (MQ=2)

                                                                                                     gaaaCGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTTGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGCTACACACGTACTACa                      <  1:861906/138‑1 (MQ=2)

                                                                                                      cAACGAGCGCAACCCTTATCTTTTGTTGCCAGC‑GGTCCGGCCGGGAACTCAAAGGAGACTGCCAGTGATAAACTGGAGGAAGGTGGGGATGACGTCAAGTCATCATGGCCCTTACGACCAGGGCTACACACGTGCTACaa                     >  2:2146768/1‑140 (MQ=33)

                                                                                                      cAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTTGGGTGGGCACTCTAA‑‑AGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGCTACACACGTACTACAATg                   <  1:660092/140‑1 (MQ=2)

                                                                                                      cAACGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGCTACACACGTACTACaa                     >  2:483219/1‑140 (MQ=2)

                                                                                                        aCGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGCTACACACGTACTACAATg                   <  2:348142/140‑1 (MQ=2)

                                                                                                         cGAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGCTACACACGTACTACAATgg                  >  2:2191627/1‑140 (MQ=2)

                                                                                                          gAGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAGAAAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGCTACACACGTACTACAATGgc                 <  1:237002/140‑1 (MQ=2)

                                                                                                           aGCGCAACCCCTATTTTTAGTTGCCAGC‑ACTTTGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGCTACACACGTACTACAATGgcc                >  2:2008989/1‑139 (MQ=2)

                                                                                                            gcgcAACCCCTATTTTTAGTTGCCAGC‑ACTTTGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGCTACACACGTACTACAATGgcca               <  1:42039/140‑3 (MQ=2)

                                                                                                             cgcAACCCCTATTTTTAGTTGCCAGC‑ACTTTGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGCTACACACGTACTACAATGgccag              >  1:1550146/1‑137 (MQ=2)

                                                                                                             cgcAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGCTacac                                >  2:702282/1‑122 (MQ=2)

                                                                                                             cgcAACCCCTATTTTTAGTTGCCAGC‑ACTTCGGGTGGGCACTCTAAAGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGCTACACACGTACTACAATGgccag              >  1:369101/1‑137 (MQ=2)

                                                                                                                  cccTTATCTTTTGTTGCCAGC‑GGTCCGGCCGGGAACTCAAAGGAGACTGCCAGTGATAAACTGGAGGAAGGTGGGGATGACGTCAAGTCATCATGGCCCTTACGACCAGGGCTACACACGTGCTACAATGGCGCATACaa         >  1:1876312/1‑140 (MQ=33)

CCACAGAACTTTCCAGAGATGGATTGGTGCCTTCGGGAACTGTGAGACAGGTGCTGCATGGCTGTCGTCAGCTCGTGTTGTGAAATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATCTTTTGTTGCCAGC‑GGTCCGGCCGGGAACTCAAAGGAGACTGCCAGTGATAAACTGGAGGAAGGTGGGGATGACGTCAAGTCATCATGGCCCTTACGACCAGGGCTACACACGTGCTACAATGGCGCATACAAAGAGAAG  >  NC_000913/224768‑225028

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`