Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,280,722	G→C	pseudogene (22/402 nt)	agaW →	N‑acetyl‑D‑galactosamine specific PTS truncated enzyme IIC component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,280,722	0	G	C	100.0%	308.4 / NA	80	pseudogene (22/402 nt)	agaW	N‑acetyl‑D‑galactosamine specific PTS truncated enzyme IIC component
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (41/39);  total (41/39)

GCGGGCGATATCGCAGCATTTAACGACCTGAAAACCGCTGGGGTGGAATGCTTCGTTCAGGGCGTCCCGACAGAGCCTGCTGTGGACCTCTTTAAATTACTTTGAGGGATTCATCATGGAAATCAGCCTGTTGCAGGCATTTGCGTTGGGCATTATCGCCTTTATCGCTGGCCTGGATATGTTTAACGGCCTAACCCATATGCACCGCCCGGTGGTCCTCGGCCCGTTGGTCGGGCTGGTACTTGGCGATCTGCATACCGGAATTTTAACCGG  >  NC_000913/3280586‑3280858                                                                                                                                         |                                                                                                                                         gCGGGCGATATCGCAGCATTTAACGACCTGAAAACCGCTGGGGTGGAATGCTTCGTTCAGGGCGTCCCGACAGAGCCTGCTGTGGACCTCTTTAAATTACTTTGAGGGATTCATCATGGAAATCAGCCTGTTGCAGCCAt                                                                                                                                       <  1:1535079/140‑1 (MQ=255)      tgATATCGCAGCATTTAACGACCTGAAAACCGCTGGGGTGGAATGCTTCGTTCAGGGCGTCCCGACAGAGCCTGCTGTGCACCTCTTTAAATTACTTTGAGGGATTCATCATGGAAATCAGCCTGTTGCAGCCATTTGCg                                                                                                                                  >  1:116535/2‑140 (MQ=255)      cGATATCGCAGCATTTAACGACCTGAAAACCGCTGGGGTGGAATGCTTCGTTCAGGGCGTCCCGACAGAGCCTGCTGTGGACCTCTTTAAATTACTTTGAGGGATTCATCATGGAAATCAGCCTGTTGCAGCCATTTGCg                                                                                                                                  <  1:429772/140‑1 (MQ=255)                 caTTTAACGACCTGAAAACCGCTGGGGTGGAATGCTTCGTTCAGGGCGTCCCGACAGAGCCTGCTGTGGACCTCTTTAAATTACTTTGAGGGATTCATCATGGAAATCAGCCTGTTGCAGCCATTTGCGTTGGGCATTAt                                                                                                                       >  2:1129587/1‑140 (MQ=255)                 caTTTAACGACCTGAAAACCGCTGGGGTGGAATGCTTCGTTCAGGGCGTCCCGACAGAGCCTGCTGTGGACCTCTTTAAATTACTTTGAGGGATTCATCATGGAAATCAGCCTGTTGCAGCCATTTGCGTTGGGCATTAt                                                                                                                       >  2:929271/1‑140 (MQ=255)                 caTTTAACGACCTGAAAACCGCTGGGGTGGAATGCTTCGTTCAGGGCGTCCCGACAGAGCCTGCTGTGGACCTCTTTAAATTACTTTGAGGGATTCATCATGGAAATCAGCCTGTTGCAGCCATTTGCGTTGGGCATTAt                                                                                                                       <  2:76659/140‑1 (MQ=255)                   tttAACGACCTGAAAACCGCTGGGGTGGAATGCTTCGTTCAGGGCGTCCCGACAGAGCCTGCTGTGGACCTCTTTAAATTACTTTGAGGGATTCATCATGGAAATCAGCCTGTTGCAGCCATTTGCGTTGGGCATTATCg                                                                                                                     >  1:1320679/1‑140 (MQ=255)                       aCGACCTGAAAACCGCTGGGGTGGAATGCTTCGTTCAGGGCGTCCCGACAGAGCCTGCTGTGGACCTCTTTAAATTACTTTGAGGGATTCATCATGGAAATCAGCCTGTTGCAGCCATTTGCGTTGGGCATTATCGCCtt                                                                                                                 <  1:929271/140‑1 (MQ=255)                           ccTGAAAACCGCTGGGGTGGAATGCTTCGTTCAGGGCGTCCCGACAGAGCCTGCTGTGGACCTCTTTAAATTACTTTGAGGGATTCATCATGGAAATCAGCCTGTTGCAGCCATTTGCGTTGGGCATTATCGCCTTTATc                                                                                                             >  1:469404/1‑140 (MQ=255)                           ccTGAAAACCGCTGGGGTGGAATGCTTCGTTCAGGGCGTCCCGACAGAGCCTGCTGTGGACCTCTTTAAATTACTTTGAGGGATTCATCATGGAAATCAGCCTGTTGCAGCCATTTGCGTTGGGCATTATCGCCTTTATc                                                                                                             >  2:74371/1‑140 (MQ=255)                           ccTGAAAACCGCTGGGGTGGAATGCTTCGTTCAGGGCGTCCCGACAGAGCCTGCTGTGGACCTCTTTAAATTACTTTGAGGGATTCATCATGGAAATCAGCCTGTTGCAGCCATTTGCGTTGGGCATTATCGCCTTTATc                                                                                                             >  2:82134/1‑140 (MQ=255)                           ccTGAAAACCGCTGGGGTGGAATGCTTCGTTCAGGGCGTCCCGACAGAGCCTGCTGTGGACCTCTTTAAATTACTTTGAGGGATTCATCATGGAAATCAGCCTGTTGCAGCCATTTGCGTTGGGCATTATCGCCTTTATc                                                                                                             >  2:1129001/1‑140 (MQ=255)                           ccTGAAAACCGCTGGGGTGGAATGCTTCGTTCAGGGCGTCCCGACAGAGCCTGCTGTGGACCTCTTTAAATTACTTTGAGGGATTCATCATGGAAATCAGCCTGTTGCAGCCATTTGCGTTGGGCATTATCGCCTTTATc                                                                                                             >  2:1198754/1‑140 (MQ=255)                           ccTGAAAACCGCTGGGGTGGAATGCTTCGTTCAGGGCGTCCCGACAGAGCCTGCTGTGGACCTCTTTAAATTACTTTGAGGGATTCATCATGGAAATCAGCCTGTTGCAGCCATTTGCGTTGGGCATTATCGCCTTTATc                                                                                                             >  1:76396/1‑140 (MQ=255)                            cTGAAAACCGCTGGGGTGGAATGCTTCGTTCAGGGCGTCCCGACAGAGCCTGCTGTGGACCTCTTTAAATTACTTTGAGGGATTCATCATGGAAATCAGCCTGTTGCAGCCATTTGCGTTGGGCATTATCGCCTTTATCg                                                                                                            <  1:291884/140‑1 (MQ=255)                             tGAAAACCGCTGGGGTGGAATGCTTCGTTCAGGGCGTCCCGACAGAGCCTGCTGTGGAGCTCTTTAAATTACTTTGAGGGATTCATCATGGAAATCAGCCTGTTGCAGCCATTTGCGTTGGGCATTATCGCCTTTATCGc                                                                                                           <  1:1044708/140‑1 (MQ=255)                             tGAAAACCGCTGGGGTGGAATGCTTCGTTCAGGGCGTCCCGACAGAGCCTGCTGTGGACCTCTTTAAATTACTTTGAGGGATTCATCATGGAAATCAGCCTGTTGCAGCCATTTGCGTTGGGCATTATCGCCTTTATCGc                                                                                                           <  2:393577/140‑1 (MQ=255)                             tGAAAACCGCTGGGGTGGAATGCTTCGTTCAGGGCGTCCCGACAGAGCCTGCTGTGCACCTCTTTAAATTACTTTGAGGGATTCATCATGGAAATCAGCCTGTTGCAGCCATTTGCGTTGGGCATTATCGCCTTTATCGc                                                                                                           <  2:116535/140‑1 (MQ=255)                                 aaCCGCTGGGGTGGAATGCTTCGTTCAGGGCGTCCCGACAGAGCCTGCTGTGGACCTCTTTAAATTACTTTGAGGGATTCATCATGGAAATCAGCCTGTTGCAGCCATTTGCGTTGGGCATTATCGCCTTTATCGCTGGc                                                                                                       <  1:74371/140‑1 (MQ=255)                                  aCCGCTGGGGTGGAATGCTTCGTTCAGGGCGTCCCGACAGAGCCTGCTGTGGACCTCTTTAAATTACTTTGAGGGATTCATCATGGAAATCAGCCTGTTGCAGCCATTTGCGTTGGGCATTATCGCCTTTATCGCTGGcc                                                                                                      <  2:1320679/140‑1 (MQ=255)                                  aCCGCTGGGGTGGAATGCTTCGTTCAGGGCGTCCCGACAGAGCCTGCTGTGGACCTCTTTAAATTACTTTGAGGGATTAATCATGGAAATCAGCCTGTTGCAGCCATTTGCGTTGGGCATTATCGCCTTTATCGCTGGcc                                                                                                      <  1:82311/140‑1 (MQ=255)                                      cTGGGGTGGAATGCTTCGTTCAGGGCGTCCCGACAGAGCCTGCTGTGGACCTCTTTAAATTACTTTGAGGGATTCATCATGGAAATCAGCCTGTTGCAGCCATTTGCGTTGGGCATTATCGCCTTTATCGCTGGCCTGGa                                                                                                  >  1:1392718/1‑140 (MQ=255)                                      cTGGGGTGGAATGCTTCGTTCAGGGCGTCCCGACAGAGCCTGCTGTGGACCTCTTTAAATTACTTTGAGGGATTCATCATGGAAATCAGCCTGTTGCAGCCATTTGCGTTGGGCATTATCGCCTTTATCGCTGGCCTGGa                                                                                                  >  2:874481/1‑140 (MQ=255)                                        ggggTGGAATGCTTCGTTCAGGGCGTCCCGACAGAGCCTGCTGTGGACCTCTTTAAATTACTTTGAGGGATTCATCATGGAAATCAGCCTGTTGCAGCCATTTGCGTTGGGCATTATCGCCTTTATCGCTGGCCTGGata                                                                                                <  1:82134/140‑1 (MQ=255)                                              gAATGCTTCGTTCAGGGCGTCCCGACAGAGCCTGCTGTGGACCTCTTTAAATTACTTTGAGGGATTCATCATGGAAATCAGCCTGTTGCAGCCATTTGCGTTGGGCATTATCGCCTTTATCGCTGGCCTGGATATGTTTa                                                                                          <  2:469404/140‑1 (MQ=255)                                               aaTGCTTCGTTCAGGGCGTCCCGACAGAGCCTGCTGTGGACCTCTTTAAATTACTTTGAGGGATTCATCATGGAAATCAGCCTGTTGCAGCCATTTGCGTTGGGCATTATCGCCTTTATCGCTGGCCTGGATATGTTTaa                                                                                         >  2:935102/1‑140 (MQ=255)                                                   cTTCGTTCAGGGCGTCCCGACAGAGCCTGCTGTGGACCTCTTTAAATTACTTTGAGGGATTCATCATGGAAATCAGCCTGTTGCAGCCATTTGCGTTGGGCATTATCGCCTTTATCGCTGGCCTGGATATGTTTAACGGc                                                                                     >  1:45996/1‑140 (MQ=255)                                                   cTTCGTTCAGGGCGTCCCGACAGAGCCTGCTGTGGACCTCTTTAAATTACTTTGAGGGATTCATCATGGAAATCAGCCTGTTGCAGCCATTTGCGTTGGGCATTATCGCCTTTATCGCTGGCCTGGATATGTTTAACGGc                                                                                     >  1:156673/1‑140 (MQ=255)                                                   cTTCGTTCAGGGCGTCCCGACAGAGCCTGCTGTGGACCTCTTTAAATTACTTTGAGGGATTCATCATGGAAATCAGCCTGTTGCAGCCATTTGCGTTGGGCATTATCGCCTTTATCGCTGGCCTGGATATGTTTAACGGc                                                                                     >  1:1116046/1‑140 (MQ=255)                                                   cTTCGTTCAGGGCGTCCCGACAGAGCCTGCTGTGGACCTCTTTAAATTACTTTGAGGGATTCATCATGGAAATCAGCCTGTTGCAGCCATTTGCGTTGGGCATTATCGCCTTTATCGCTGGCCTGGATATGTTTAACGGc                                                                                     <  2:45996/140‑1 (MQ=255)                                                   cTTCGTTCAGGGCGTCCCGACAGAGCCTGCTGTGGACCTCTTTAAATTACTTTGAGGGATTCATCATGGAAATCAGCCTGTTGCAGCCATTTGCGTTGGGCATTATCGCCTTTATCGCTGGCCTGGATATGTTTAACGGc                                                                                     <  1:1198754/140‑1 (MQ=255)                                                     tCGTTCAGGGCGTCCCGACAGAGCCTGCTGTGGACCTCTTTAAATTACTTTGAGGGATTCATCATGGAAATCAGCCTGTTGCAGCCATTTGCGTTGGGCATTATCGCCTTTATCGCTGGCCTGGATATGTTTAACGGCCt                                                                                   <  2:1610281/140‑1 (MQ=255)                                                     tCGTTCAGGGCGTCCCGACAGAGCCTGCTGTGGACCTCTTTAAATTACTTTGAGGGATTCATCATGGAAATCAGCCTGTTGCAGCCATTTGCGTTGGGCATTATCGCCTTTATCGCTGGCCTGGATATGTTTAACGGCCt                                                                                   <  1:70474/140‑1 (MQ=255)                                                             ggCGTCCCGACAGAGCCTGCTGTGGACCTCTTTAAATTACTTTGAGGGATTCATCATGGAAATCAGCCTGTTGCAGCCATTTGCGTTGGGCATTATCGCCTTTATCGCTGGCCTGGATATGTTTAACGGCCTAACCCata                                                                           >  1:988390/1‑140 (MQ=255)                                                                 tCCCGACAGAGCCTGCTGTGGACCTCTTTAAATTACTTTGAGGGATTCATCATGGAAATCAGCCTGTTGCAGCCATTTGCGTTGGGCATTATCGCCTTTATCGCTGGCCTGGATATGTTTAACGGCCTAACCCATATGCa                                                                       <  2:1050363/140‑1 (MQ=255)                                                                       cAGAGCCTGCTGTGGACCTCTTTAAATTACTTTGAGGGATTCATCATGGAAATCAGCCTGTTGCAGCCATTTGCGTTGGGCATTATCGCCTTTATCGCTGGCCTGGATATGTTTAACGGCCTAACCCATATGCACCGccc                                                                 >  2:407359/1‑140 (MQ=255)                                                                          agCCTGCTGTGGACCTCTTTAAATTACTTTGAGGGATTCATCATGGAAATCAGCCTGTTGCAGCCATTTGCGTTGGGCATTATCGCCTTTATCGCTGGCCTGGATATGTTTAACGGCCTAACCCATATGCACCGCCCggt                                                              <  2:24020/140‑1 (MQ=255)                                                                          agCCTGCTGTGGACCTCTTTAAATTACTTTGAGGGATTCATCATGGAAATCAGCCTGTTGCAGCCATTTGCGTTGGGCATTATCGCCTTTATCGCTGGCCTGGATATG‑TTAACGGCCTAACCCATATGCACCGCCCggtg                                                             >  2:138046/1‑140 (MQ=255)                                                                            ccTGCTGTGGACCTCTTTAAATTACTTTGAGGGATTCATCATGGAAATCAGCCTGTTGCAGCCATTTGCGTTGGGCATTATCGCCTTTATCGCTGGCCTGGATATGTTTAACGGCCTAACCCATATGCACCGCCCggtgg                                                            >  2:280913/1‑140 (MQ=255)                                                                            ccTGCTGTGGACCTCTTTAAATTACTTTGAGGGATTCATCATGGAAATCAGCCTGTTGCAGCCATTTGCGTTGGGCATTATCGCCTTTATCGCTGGCCTGGATATGTTTAACGGCCTAACCCATATGCACCGCCCggtgg                                                            >  1:967337/1‑140 (MQ=255)                                                                            ccTGCTGTGGACCTCTTTAAATTACTTTGAGGGATTCATCATGGAAATCAGCCTGTTGCAGCCATTTGCGTTGGGCATTATCGCCTTTATCGCTGGCCTGGATATGTTTAACGGCCTAACCCATATGCACCGCCCggtgg                                                            >  1:1308958/1‑140 (MQ=255)                                                                             ctgctgTGGACCTCTTTAAATTACTTTGAGGGATTCATCATGGAAATCAGCCTGTTGCAGCCATTTGCGTTGGGCATTATCGCCTTTATCGCTGGCCTGGATATGTTTAACGGCCTAACCCATATGCACCGCCCggtggt                                                           >  1:1024866/1‑140 (MQ=255)                                                                                 tgtgGACCTCTTTAAATTACTTTGAGGGATTCATCATGGAAATCAGCCTGTTGCAGCCATTTGCGTTGGGCATTATCGCCTTTATCGCTGGCCTGGATATGTTTAACGGCCTAACCCATATGCACCGCCCGGTGGTCCTc                                                       >  2:1220096/1‑140 (MQ=255)                                                                                    ggACCTCTTTAAATTACTTTGAGGGATTCATCATGGAAATCAGCCTGTTGCAGCCATTTGCGTTGGGCATTATCGCCTTTATCGCTGGCCTGGATATGTTTAACGGCCTAACCCATATGCACCGCCCGGTGGTCCTCGGc                                                    >  1:573388/1‑140 (MQ=255)                                                                                     gACCTCTTTAAATTACTTTGAGGGATTCATCATGGAAATCAGCCTGTTGCAGCCATTTGCGTTGGGCATTATCGCCTTTATCGCTGGCCTGGATATGTTTAACGGCCTAACCCATATGCACCGCCCGGTGGTCCTCg                                                      >  1:177535/1‑137 (MQ=255)                                                                                     gACCTCTTTAAATTACTTTGAGGGATTCATCATGGAAATCAGCCTGTTGCAGCCATTTGCGTTGGGCATTATCGCCTTTATCGCTGGCCTGGATATGTTTAACGGCCTAACCCATATGCACCGCCCGGTGGTCCTCg                                                      <  2:177535/137‑1 (MQ=255)                                                                                     gACCTCTTTAAATTACTTTGAGGGATTCATCATGGAAATCAGCCTGTTGCAGCCATTTGCGTTGGGCATTATCGCCTTTATCGCTGGCCTGGATATGTTTAACGGCCTAACCCATATGCACCGCCCGGTGGTCCTCGGcc                                                   >  1:1586058/1‑140 (MQ=255)                                                                                     gACCTCTTTAAATTACTTTGAGGGATTCATCATGGAAATCAGCCTGTTGCAGCCATTTGCGTTGGGCATTATCGCCTTTATCGCTGGCCTGGATATGTTTAACGGCCTAACCCATATGCACCGCCCGGTGGTCCTCGGcc                                                   >  1:47019/1‑140 (MQ=255)                                                                                      aCCTCTTTAAATTACTTTGAGGGATTCATCATGGAAATCAGCCTGTTGCAGCCATTTGCGTTGGGCATTATCGCCTTTATCGCTGGCCTGGATATGTTTAACGGCCTAACCCATATGCACCGCCCGGTGGTCCTCGGccc                                                  <  1:1220096/140‑1 (MQ=255)                                                                                        ctctTTAAATTACTTTGAGGGATTCATCATGGAAATCAGCCTGTTGCAGCCATTTGCGTTGGGCATTATCGCCTTTATCGCTGGCCTGGATATGTTTAACGGCCTAACCCATATGCACCGCCCGGTGGTCCTCGGCCCGt                                                <  2:47019/140‑1 (MQ=255)                                                                                         tctTTAAATTACTTTGAGGGATTCATCATGGAAATCAGCCTGTTGCAGCCATTTGCGTTGGGCATTATCGCCTTTATCGCTGGCCTGGATATGTTTAACGGCCTAACCCATATGCACCGCCCGGTGGTCCTCGGCCCGtt                                               >  1:87553/1‑140 (MQ=255)                                                                                            ttAAATTACTTTGAGGGATTCATCATGGAAATCAGCCTGTTGCAGCCATTTGCGTTGGGCATTATCGCCTTTATCGCTGGCCTGGATATGTTTAACGGCCTAACCCATATGCACCGCCCGGTGGTCCTCGGCCCGTTGGt                                            <  1:874481/140‑1 (MQ=255)                                                                                            ttAAATTACTTTGAGGGATTCATCATGGAAATCAGCCTGTTGCAGCCATTTGCGTTGGGCATTATCGCCTTTATCGCTGGCCTGGATATGTTTAACGGCCTAACCCATATGCACCGCCCGGTGGTCCTCGGCCCGTTGGt                                            <  2:76396/140‑1 (MQ=255)                                                                                            ttAAATTACTTTGAGGGATTCATCATGGAAATCAGCCTGTTGCAGCCATTTGCGTTGGGCATTATCGCCTTTATCGCTGGCCTGGATATGTTTAACGGCCTAACCCATATGCACCGCCCGGTGGTCCTCGGCCCGTTGGt                                            <  2:1586058/140‑1 (MQ=255)                                                                                               aaTTACTTTGAGGGATTCATCATGGAAATCAGCCTGTTGCAGCCATTTGCGTTGGGCATTATCGCCTTTATCGCTGGCCTGGATATGTTTAACGGCCTAACCCATATGCACCGCCCGGTGGTCCTCGGCCCGTTGGTCgg                                         >  2:79004/1‑140 (MQ=255)                                                                                                   aCTTTGAGGGATTCATCATGGAAATCAGCCTGTTGCAGCCATTTGCGTTGGGCATTATCGCCTTTATCGCTGGCCTGGATATGTTTAACGGCCTAACCCATATGCACCGCCCGGTGGTCCTCGGCCCGTTGGTCGGGCTg                                     <  2:156673/140‑1 (MQ=255)                                                                                                   aCTTTGAGGGATTCATCATGGAAATCAGCCTGTTGCAGCCATTTGCGTTGGGCATTATCGCCTTTATCGCTGGCCTGGATATGTTTAACGGCCTAACCCATATGCACCGCCCGGTGGTCCTCGGCCCGTTGGTCGGGCTg                                     <  2:1367282/140‑1 (MQ=255)                                                                                                    cTTTGAGGGATTCATCATGGAAATCAGCCTGTTGCAGCCATTTGCGTTGGGCATTATCGCCTTTATCGCTGGCCTGGATATGTTTAACGGCCTAACCCATATGCACCGCCCGGTGGTCCTCGGCCCGTTGGTCGGGCTgg                                    >  1:582353/1‑140 (MQ=255)                                                                                                     tttGAGGGATTCATCATGGAAATCAGCCTGTTGCAGCCATTTGCGTTGGGCATTATCGCCTTTATCGCTGGCCTGGATGTGTTTAACGGCCTAACCCATATGCACCGCCCGGTGGTCCTCGGCCCGTTGGTCGGGc                                       >  1:958184/1‑136 (MQ=255)                                                                                                     tttGAGGGATTCATCATGGAAATCAGCCTGTTGCAGCCATTTGCGTTGGGCATTATCGCCTTTATCGCTGGCCTGGATATGTTTAACGGCCTAACCCATATGCACCGCCCGGTGGTCCTCGGCCCGTTGGTCGGGc                                       <  2:958184/136‑1 (MQ=255)                                                                                                     tttGAGGGATTCATCATGGAAATCAGCCTGTTGCAGCCATTTGCGTTGGGCATTATCGCCTTTATCGCTGGCCTGGATATGTTTAACGGCCTAACCCATATGCACCGCCCGGTGGTCCTCGGCCCGTTGGTCGGGCTGGt                                   >  2:739980/1‑140 (MQ=255)                                                                                                     tttGAGGGATTCATCATGGAAATCAGCCTGTTGCAGCCATTTGCGTTGGGCATTATCGCCTTTATCGCTGGCCTGGATATGTTTAACGGCCTAACCCATATGCACCGCCCGGTGGTCCTCGGCCCGTTGGTCGGGCTGGt                                   >  1:1458552/1‑140 (MQ=255)                                                                                                     tttGAGGGATTCATCATGGAAATCAGCCTGTTGCAGCCATTTGCGTTGGGCATTATCGCCTTTATCGCTGGCCTGGATATGTTTAACGGCCTAACCCATATGCACCGCCCGGTGGTCCTCGGCCCGTTGGTCGGGCTGGt                                   >  2:248580/1‑140 (MQ=255)                                                                                                     tttGAGGGATTCATCATGGAAATCAGCCTGTTGCAGCCATTTGCGTTGGGCATTATCGCCTTTATCGCTGGCCTGGATATGTTTAACGGCCTAACCCATATGCACCGCCCGGTGGTCCTCGGCCCGTTGGTCGGGCTGGt                                   <  1:407359/140‑1 (MQ=255)                                                                                                         aGGGATTCATCATGGAAATCAGCCTGTTGCAGCCATTTGCGTTGGGCATTATCGCCTTTATCGCTGGCCTGGATATGTTTAACGGCCTAACCCATATGCACCGCCCGGTGGTCCTCGGCCCGTTGGt                                            >  1:56413/1‑127 (MQ=255)                                                                                                         aGGGATTCATCATGGAAATCAGCCTGTTGCAGCCATTTGCGTTGGGCATTATCGCCTTTATCGCTGGCCTGGATATGTTTAACGGCCTAACCCATATGCACCGCCCGGTGGTCCTCGGCCCGTTGGt                                            <  2:56413/127‑1 (MQ=255)                                                                                                          gggATTCATCATGGAAATCAGCCTGTTGCAGCCATTTGCGTTGGGCATTATCGCCTTTATCGCTGGCCTGGATATGTTTAACGGCCTAACCCATATGCACCGCCCGGTGGTCCTCGGCCCGTTGGTCGGGCTGGTACTTg                              <  2:967337/140‑1 (MQ=255)                                                                                                          gggATTCATCATGGAAATCAGCCTGTTGCAGCCATTTGCGTTGGGCATTATCGCCTTTATCGCTGGCCTGGATATGTTTAACGGCCTAACCCATATGCACCGCCCGGTGGTCCTCGGCCCGTTGGTCGGGCTGGTACTTg                              <  2:1458552/140‑1 (MQ=255)                                                                                                          gggATTCATCATGGAAATCAGCCTGTTGCAGCCATTTGCGTTGGGCATTATCGCCTTTATCGCTGGCCTGGATATGTTTAACGGCCTAACCCATATGCACCGCCCGGTGGTCCTCGGCCCGTTGGTCGGGCTGGTACTTg                              <  1:79004/140‑1 (MQ=255)                                                                                                            gATTCATCATGGAAATCAGCCTGTTGCAGCCATTTGCGTTGGGCATTATCGCCTTTATCGCTGGCCTGGATATGTTTAACGGCCTAACCCATATGCACCGCCCGGTGGTCCTCGGCCCGTTGGTCGGGCTGGTACTTGGc                            <  1:739980/140‑1 (MQ=255)                                                                                                              ttCATCATGGAAATCAGCCTGTTGCAGCCATTTGCGTTGGGCATTATCGCCTTTATCGCTGGCCTGGATATGTTTAACGGCCTAACCCATATGCACCGCCCGGTGGTCCTCGGCCCGTTGGTCggg                                        >  1:1500020/1‑126 (MQ=255)                                                                                                              ttCATCATGGAAATCAGCCTGTTGCAGCCATTTGCGTTGGGCATTATCGCCTTTATCGCTGGCCTGGATATGTTTAACGGCCTAACCCATATGCACCGCCCGGTGGTCCTCGGCCCGTTGGTCggg                                        <  2:1500020/126‑1 (MQ=255)                                                                                                               tcatcaTGGAAATCAGCCTGTTGCAGCCATTTGCGTTGGGCATTATCGCCTTTATCGCTGGCCTGGATATGTTTAACGGCCTAACCCATATGCACCGCCCGGTGGTCCTCGGCCCGTTGGTCGGGCTGGTACTTGGCGAt                         <  1:1129001/140‑1 (MQ=255)                                                                                                                     tGGAAATCAGCCTGTTGCAGCCATTTGCGTTGGGCATTATCGCCTTTATCGCTGGCCTGGATATGTTTAACGGCCTAACCCATATGCACCGCCCGGTGGTCCTCGGCCCGTTGGTCGGGCTGGTACTTGGCGATCTGCAt                   >  1:773782/1‑140 (MQ=255)                                                                                                                     tGGAAATCAGCCTGTTGCAGCCATTTGCGTTGGGCATTATCGCCTTTATCGCTGGCCTGGATATGTTTAACGGCCTAACCCATATGCACCGCCCGGTGGTCCTCGGCCCGTTGGTCGGGCTGGTACTTGGCGATCTGCAt                   >  2:244469/1‑140 (MQ=255)                                                                                                                      ggAAATCAGCCTGTTGCAGCCATTTGCGTTGGGCATTATCGCCTTTATCGCTGGCCTGGATATGTTTAACGGCCTAACCCATATGCACCGCCCGGTGGTCCTCGGCCCGTTGGTCGGGCTGGTACTTGGCGATCTGCATa                  >  2:914430/1‑140 (MQ=255)                                                                                                                       gAAATCAGCCTGTTGCAGCCATTTGCGTTGGGCATTATCGCCTTTATCGCTGGCCTGGATATGTTTAACGGCCTAACCCATATGCACCGCCCGGTGGTCCTCGGCCCGTTGGTCGGGCTGGTACTTGGCGATCTGCa                    <  1:1067890/137‑1 (MQ=255)                                                                                                                       gAAATCAGCCTGTTGCAGCCATTTGCGTTGGGCATTATCGCCTTTATCGCTGGCCTGGATATGTTTAACGGCCTAACCCATATGCACCGCCCGGTGGTCCTCGGCCCGTTGGTCGGGCTGGTACTTGGCGATCTGCa                    >  2:1067890/1‑137 (MQ=255)                                                                                                                        aaaTCAGCCTGTTGCAGCCATTTGCGTTGGGCATTATCGCCTTTATCGCTGGCCTGGATATGTTTAACGGCCTAACCCATATGCACCGCCCGGTGGTCCTCGGCCCGTTGGTCGGGCTGGTACTTGGCGATCTGCATAcc                <  1:1129587/140‑1 (MQ=255)                                                                                                                                cTGTTGCAGCCATTTGCGTTGGGCATTATCGCCTTTATCGCTGGCCTGGATATGTTTAACGGCCTAACCCATATGCACCGCCCGGTGGTCCTCGGCCCGTTGGTCGGGCTGGTACTTGGCGATCTGCATACCGGAAtttt        >  1:1315054/1‑140 (MQ=255)                                                                                                                                      cAGCCATTTGCGTTGGGCATTATCGCCTTTATCGCTGGCCTGGATATGTTTAACGGCCTAACCCATATGCACCGCCCGGTGGTCCTCGGCCCGTTGGTCGGGCTGGTACTTGGCGATCTGCATACCGGAATTTTAACcgg  <  2:988390/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                         GCGGGCGATATCGCAGCATTTAACGACCTGAAAACCGCTGGGGTGGAATGCTTCGTTCAGGGCGTCCCGACAGAGCCTGCTGTGGACCTCTTTAAATTACTTTGAGGGATTCATCATGGAAATCAGCCTGTTGCAGGCATTTGCGTTGGGCATTATCGCCTTTATCGCTGGCCTGGATATGTTTAACGGCCTAACCCATATGCACCGCCCGGTGGTCCTCGGCCCGTTGGTCGGGCTGGTACTTGGCGATCTGCATACCGGAATTTTAACCGG  >  NC_000913/3280586‑3280858

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑