Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,604,3440CT35.4% 134.9 / 59.8 99S62F (TCT→TTT) bglJDNA‑binding transcriptional regulator BglJ
Reads supporting (aligned to +/- strand):  ref base C (15/49);  new base T (35/0);  total (50/49)
Fisher's exact test for biased strand distribution p-value = 4.11e-15
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 113 total aligned reads displayed.

AAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTG‑CCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCAT  >  NC_000913/4604205‑4604481
                                                                                                                                           |                                                                                                                                          
aaaTGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCATTTGCGGCGGTCATtttttc                                                                                                                                            <  1:415416/140‑1 (MQ=255)
  aTGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATtttttttt                                                                                                                                          >  2:1349519/1‑140 (MQ=255)
  aTGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATtttttctt                                                                                                                                          >  1:1082412/1‑140 (MQ=255)
     cGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATtttttcttttt                                                                                                                                       <  1:79858/140‑1 (MQ=255)
     cGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATtttttcttttt                                                                                                                                       <  2:126950/140‑1 (MQ=255)
     cGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATtttttcttttt                                                                                                                                       <  1:1462442/140‑1 (MQ=255)
       tCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTttttttttttct                                                                                                                                     >  2:748736/1‑140 (MQ=255)
       tCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTttttctttttct                                                                                                                                     <  2:835370/140‑1 (MQ=255)
            agtagtATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTG‑CCAt                                                                                                                               <  2:1080211/140‑1 (MQ=255)
                   tCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCAtttttttttttt                                                                                                                                       >  2:650646/1‑126 (MQ=255)
                   tCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATtttttcttttt                                                                                                                                       <  1:650646/126‑1 (MQ=255)
                   tCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTG‑CCATGAGAAGt                                                                                                                        <  1:778569/140‑1 (MQ=255)
                   tCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTG‑CCATGAGAAGt                                                                                                                        <  1:382499/140‑1 (MQ=255)
                   tCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTG‑CCATGAGAAGt                                                                                                                        <  1:686381/140‑1 (MQ=255)
                    cGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTCTG‑CCATGAGAAGTg                                                                                                                       >  1:901163/1‑140 (MQ=255)
                      gTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTT‑CCATGTGAAGGGAg                                                                                                                     >  2:654182/1‑140 (MQ=255)
                      gTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTT‑CCATGAGAAATggg                                                                                                                     >  2:382499/1‑138 (MQ=255)
                      gTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTG‑CCCTGAGAAATGAg                                                                                                                     >  2:483672/1‑140 (MQ=255)
                      gTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTG‑CCATGAGAAGTGAg                                                                                                                     <  1:1328267/140‑1 (MQ=255)
                      gTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTG‑CCATGAGAAGTGAg                                                                                                                     <  2:1007775/140‑1 (MQ=255)
                       tATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTG‑CCCTGAGAAGTGAgg                                                                                                                    >  2:1108737/1‑139 (MQ=255)
                        aTTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTttttctttttct                                                                                                                                     <  1:114817/123‑1 (MQ=255)
                        aTTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTT‑CCATGAGGAGTGAgcg                                                                                                                   >  2:1328267/1‑140 (MQ=255)
                        aTTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTT‑CCATGAGAAGTGGgcg                                                                                                                   >  2:1154247/1‑140 (MQ=255)
                         ttGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTCTG‑CCATGAGAAGTGAgcgc                                                                                                                  >  1:150946/1‑140 (MQ=255)
                         ttGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTCTG‑CCATGAGAAGTGAgcgc                                                                                                                  >  1:1326604/1‑140 (MQ=255)
                           gagaGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTTTT‑CCATGAGAAGTGAGCGCag                                                                                                                >  1:416750/1‑140 (MQ=255)
                           gagaGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTG‑CCATGAGAAGTGAGCGCag                                                                                                                <  1:1154247/140‑1 (MQ=255)
                           gagaGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTG‑CCATGAGAAGTGAGCGCag                                                                                                                >  1:964929/1‑140 (MQ=255)
                            agagTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTG‑CCATGAGAAGTGAGCGCaga                                                                                                               <  1:36060/140‑1 (MQ=255)
                             gagTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTG‑CCATGAGAAGTGAGCGCagag                                                                                                              <  2:1185839/140‑1 (MQ=255)
                             gagTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGAATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTG‑CCATGAGAAGTGAGCGCagag                                                                                                              <  1:1156459/140‑1 (MQ=255)
                                 ttATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTT‑CCCTGGGAAGGGGGGGCCGGGAggg                                                                                                          >  2:851363/1‑140 (MQ=255)
                                 ttATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTCTG‑CCATGAGAAGTGAGCGCAGAGAggg                                                                                                          >  2:25136/1‑140 (MQ=255)
                                 ttATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTG‑CCATGAGAAGTGAGCGCAGAGAggg                                                                                                          <  2:484147/140‑1 (MQ=255)
                                 ttATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTG‑CCATGAGAAGTGAGCGCAGAGAggg                                                                                                          >  1:1000598/1‑140 (MQ=255)
                                   aTTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTG‑CCATGAGAAGTGAGCGCAGAGAGGGa                                                                                                         <  2:153160/139‑1 (MQ=255)
                                     tCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTT‑CCATGAGGAGGGAGCGGAGAGAGGGGttt                                                                                                      >  2:36060/1‑139 (MQ=255)
                                         aaaGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGGCATTTTTTCTTTTTTTG‑CCATGAGAAGGGAGCGCACAGAGGGGTTAtttt                                                                                                  >  2:566544/1‑140 (MQ=255)
                                          aaGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTG‑CCATGAGAAGTGAGCGCAGAGAGGGATTATCTTg                                                                                                 <  2:150946/140‑1 (MQ=255)
                                          aaGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTG‑CCATGAGAAGTGAGCGCAGAGAGGGATTATCTTg                                                                                                 <  1:566544/140‑1 (MQ=255)
                                           aGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTG‑CCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGc                                                                                                <  1:1276659/140‑1 (MQ=255)
                                               tGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTT‑TCATTAGAAGTGGGGGCAGAGAGGGGTTTTCTTTCctga                                                                                            >  2:1589022/1‑140 (MQ=255)
                                               tGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTT‑CCATGAGAAGGGAGCGCAGAGAGGGGGTATCTTGCctga                                                                                            >  2:1363551/1‑140 (MQ=255)
                                               tGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTG‑CCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCctga                                                                                            <  1:1589022/140‑1 (MQ=255)
                                                     tAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTG‑CCATGAGAAGTGAGCGCAGAGAGGGATTATTTTGCCTGACTGAAc                                                                                      >  1:1058186/1‑140 (MQ=255)
                                                     tAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTG‑CCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAAc                                                                                      <  1:25136/140‑1 (MQ=255)
                                                       gcccTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTG‑CCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTg                                                                                    <  1:1595898/139‑1 (MQ=255)
                                                       aCCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTG‑CCATGAGAAGTGAGCGCAGAGAGGGGTTATCTTGCCTGACTGAACTg                                                                                    >  1:465756/1‑140 (MQ=255)
                                                            tataAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTG‑CCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGAt                                                                               <  1:748736/140‑1 (MQ=255)
                                                                  cTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTG‑CCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGtt                                                                         <  1:1140301/140‑1 (MQ=255)
                                                                   tCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTT‑CCATGGGAAGTGAGCGCAGGGAGGGGTTTTTTTGCCTGACTGAACTTGCGGTTaaagtt                                                                        >  2:588829/1‑136 (MQ=255)
                                                                   tCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTG‑CCCTTAGGAGGGAGGGCAGAGAGGGGGTATCTTTCCCGACTGGACTGGCGGTTTAAttt                                                                        >  2:1595898/1‑140 (MQ=255)
                                                                     cATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTT‑CCATGAGAAGGGAGCGCAGAGAGGGGTTTTTTTGCCTGACTTAACTGGCGGTTAAGttttc                                                                      >  2:89010/1‑138 (MQ=255)
                                                                      aTACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTT‑CCCTGAGAAGTGAGCGCAGAGGGGGGTTATCTTGGCTGACTGGACTGGCGGTTAAATTTCcg                                                                     >  2:909359/1‑140 (MQ=255)
                                                                            ataCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTG‑CCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTAcc                                                               <  2:1082412/140‑1 (MQ=255)
                                                                              aCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTG‑CCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCg                                                             <  2:1326604/140‑1 (MQ=255)
                                                                              aCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTG‑CCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCg                                                             <  1:851363/140‑1 (MQ=255)
                                                                               ccAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTG‑CCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCgg                                                            <  1:937081/140‑1 (MQ=255)
                                                                                 aGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTG‑CCCTGAGAAGTGAGGGCAGAGGGGGGTTTTTTTTCCTGACTGAACCGGCGAGTAAGGTTTCGCGGACCCcgcg                                                          >  2:60703/1‑140 (MQ=255)
                                                                                 aGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTG‑CCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCg                                                          <  1:472696/140‑1 (MQ=255)
                                                                                  gTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTT‑CCCTGAGAAGGGAGCGCAGAGGGGGAGTATTTTTCCTTACTGAACTGGCGATTAAGGTTTCGCGTACCCcgcgg                                                         >  2:573741/1‑139 (MQ=255)
                                                                                  gTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTCTT‑CCCTGAGAAGTGAGCGCAGGGGGGGGTTATCTTTCCTGACTGAACTGGCGATTAAGTTTTCGCGTACCCggggg                                                         >  2:156988/1‑137 (MQ=255)
                                                                                  gTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTTTT‑CCATGAGAAGTGAGGGCAGAGAGGGATTTTCTTTCCTGACTGAACTGGCGCTTAAGTTTCCGCGTAACCcgcgg                                                         >  2:955039/1‑139 (MQ=255)
                                                                                  gTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTG‑CCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGt                                                         >  2:1021023/1‑140 (MQ=255)
                                                                                   tCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTTTG‑CCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGtt                                                        >  1:1353038/1‑140 (MQ=255)
                                                                                   tCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTG‑CCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTAcc                                                               >  2:637419/1‑133 (MQ=255)
                                                                                   tCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTG‑CCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTAcc                                                               <  1:637419/133‑1 (MQ=255)
                                                                                    cAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTGATTTTTTCTTTTTTTT‑TCCTGAGGAGTGAGCGCCGGGCGGGGGTATCTTTCCTGACCGTACACGCGATTAAGTTTCCCCGCAACCCGCGttt                                                       >  2:288788/1‑140 (MQ=255)
                                                                                    cAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTT‑CCCTGAGAAGTGAGCGCCGAGGGGGGTTATTTTTCCTGACTGAACTGGGGATTAAGTTTTCGCGGACCCGGCGgtt                                                       >  2:472696/1‑140 (MQ=255)
                                                                                     aGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTT‑CCATGAGAAGGGAGCGCAGAGAGGGATTATCTTTCCTGACTGAACTGGCGATTAAGTTTTCGCGGACCCCGCGtttt                                                      >  2:1468078/1‑139 (MQ=255)
                                                                                       gAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTG‑CCATGAGAAGTGAGGGCGGAGAGGGGTTATTTTGCCTTACTGAACTGGCGGTTAAGTTTTCGCGTACCCGGGGTTTTGt                                                    >  2:1581335/1‑140 (MQ=255)
                                                                                           cATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTG‑CCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTAtt                                                <  1:551257/140‑1 (MQ=255)
                                                                                            aTTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTTTG‑CCATGAGAAGTGAGCGCCGAGGGGGGGTATCTTTCCTGACTGAACTGGCGATTAAATTTTCGCGTACCCCGCGTTTTGTttttt                                               >  2:773304/1‑139 (MQ=255)
                                                                                            aTTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTG‑CCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTg                                               <  1:1363551/140‑1 (MQ=255)
                                                                                            aTTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTG‑CCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTg                                               <  1:1468078/140‑1 (MQ=255)
                                                                                            aTTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTG‑CCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTg                                               <  2:1000598/140‑1 (MQ=255)
                                                                                             tttCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTG‑CCATGAGAAGTGAGCGCAGAGAGGGATTATCTTTCCTGACTGAACTGGCGATTAAGTTTTCGCGTACCCGGCGGTTTGTTAtttg                                              >  2:551257/1‑138 (MQ=255)
                                                                                                cAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTG‑CCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGga                                           <  2:730757/140‑1 (MQ=255)
                                                                                                cAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTG‑CCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGga                                           <  1:574458/140‑1 (MQ=255)
                                                                                                 aGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTCTT‑CCCTGAGAAGTGAACGCAAGGAGGGGATATTTTTCCTGCCCGAACTGGCGAGTTAATTTTCGCGTTCCCCGCGTTTTGTTATTTTGgat                                          >  2:1082719/1‑140 (MQ=255)
                                                                                                 aGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTG‑CCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGgat                                          <  1:288788/140‑1 (MQ=255)
                                                                                                  ggATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTG‑CCATGAGAAGTGAGCGCAGAGAGGGGTTATTTTTCCTCGATGAACTGGGGATTAAGTTTTCGCGTACCCGGCGGTTAGTTTTTGCGggtg                                         >  2:252008/1‑140 (MQ=255)
                                                                                                   gATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTG‑CCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGgatga                                        <  1:94393/140‑1 (MQ=255)
                                                                                                    aTGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTG‑CCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGgatgat                                       <  1:955039/140‑1 (MQ=255)
                                                                                                    aTGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTG‑CCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGgatgat                                       <  1:909359/140‑1 (MQ=255)
                                                                                                    aTGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTG‑CCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGgatgat                                       <  2:901163/140‑1 (MQ=255)
                                                                                                     tGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGTCCAAG‑GGAGGGAAGGCAGAGCGGGGTTGTTCTTCCCGACTTGACCGGCGATTAAGATTTCGCCTGTCCCGGGTTTTGTTGTTGAGggtgaag                                      >  2:454299/1‑138 (MQ=255)
                                                                                                      gCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTG‑CCATGAGAAGGGAGCGCAGAGAGGGGTTATCTTGCCTGACTGAACTGGCGGTTAAGTTTCCGCGTACCCCGCGTTTTTTTTTTGCGgatgatga                                     >  2:547916/1‑140 (MQ=255)
                                                                                                      gCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTT‑CCCTGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTTCGgatgatga                                     >  1:629230/1‑140 (MQ=255)
                                                                                                             cgcgGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTCTT‑CCATGAGAAGTGAGGGCAGGGAGGGGTTATCTTTTCTGCCTTGAATGGGGATTAAAGTTCCGCGTACCCGGCGGTTAGTTTTTTTGGAGGATGATATTgta                              >  2:1474419/1‑138 (MQ=255)
                                                                                                              gcgGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTG‑CCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAg                             <  1:547916/140‑1 (MQ=255)
                                                                                                              gcgGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTG‑CCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACCGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAg                             <  2:917062/140‑1 (MQ=255)
                                                                                                                ggATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTG‑CCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCt                           <  1:573741/140‑1 (MQ=255)
                                                                                                                   tctcGTTTGCGGCGGTCATTTTTTCTTTTTCTG‑CCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCgg                        <  1:1021023/140‑1 (MQ=255)
                                                                                                                      cGTTTGCGGCGGTCATTTTTTTTTTTTTTT‑CCCTGAGAAGGGAGCGCCGAGAGGGGTTATCTTTCCTGTCTGAACTGGGGATTTAGTTTCCGCGTACCCCGCGGTTTGTTTTTTCGGGTGATGATATTGAAGCTCGGCTg                     >  2:392875/1‑140 (MQ=255)
                                                                                                                          tGCGGCGGTCATTTTTTTTTTTTTTG‑CCATGAGAAGGGAGCGCCGAGAGGGGTTATTTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGGACCCGGCGTTTTGTTATTTCGGATGATGATATTGAAGCTCGGCTGATTg                 >  2:214059/1‑140 (MQ=255)
                                                                                                                                 gTCATTTTTTCTTTTTTTG‑CCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTTCGGATGATGATATTGAAGCTCGGCTGATTGGTTCAtt          >  2:1300249/1‑140 (MQ=255)
                                                                                                                                 gTCATTTTTTCTTTTTCTG‑CCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCAtt          <  2:1058186/140‑1 (MQ=255)
                                                                                                                                         ttctttttctG‑CCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCAt  <  1:783817/140‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                          
AAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTG‑CCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCAT  >  NC_000913/4604205‑4604481

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑