Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,815,288	G→C	R161G (CGC→GGC)	pyrE ←	orotate phosphoribosyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,815,288	0	G	C	100.0%	328.5 / NA	85	R161G (CGC→GGC)	pyrE	orotate phosphoribosyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (44/41);  total (44/41)

GCCTTAACCGCCGCCAGATGTTCCGCCATTTCCGGCTTCTCTTCCAGGTAAGCAATCAGGTCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCGCGTTCCTGACGATCGAGCGAAATCAACACGCCAGCAAGCGTCGCGCCATTGGCCTGAATAATCTCCATCGACTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAA  >  NC_000913/3815152‑3815423                                                                                                                                         |                                                                                                                                        gCCTTAACCGCCGCCAGATGTTCCGCCATTTCCGGCTTCTCTTCCAGGTAAGCAATCAGGTCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCcccc                                                                                                                                      <  2:434452/140‑1 (MQ=255)    ttAACCGCCGCCAGATGTTCCGCCATTTCCGGCTTCTCTTCCAGGTAAGCAATCAGGTCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCCCCcgcg                                                                                                                                   <  2:516562/140‑1 (MQ=255)    ttAACCGCCGCCAGATGTTCCGCCATTTCCGGCTTCTCTTCCAGGTAAGCAATCAGGTCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCCCCcgcg                                                                                                                                   <  2:1477883/140‑1 (MQ=255)    ttAACCGCCGCCAGATGTTCCGCCATTTCCGGCTTCTCTTCCAGGTAAGCAATCAGGTCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCCCCcgcg                                                                                                                                   <  2:333389/140‑1 (MQ=255)    ttAACCGCCGCCAGATGTTCCGCCATTTCCGGCTTCTCTTCCAGGTAAGCAATCAGGTATTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCCCCcgcg                                                                                                                                   <  2:78672/140‑1 (MQ=255)           ccgccAGATGTTCCGCCATTTCCGGCTTCTCTTCCAGGTAAGCAATCAGGTCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCCCCCGCGTTCCTGa                                                                                                                            >  1:531993/1‑140 (MQ=255)            cgccAGATGTTCGGCCATTTCCGGCTTCTCTTCCAGGTAAGCAATCAGGTCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCCCCCGCGTTCCTGAc                                                                                                                           >  2:1300995/1‑140 (MQ=255)            cgccAGATGTTCCGCCATTTCCGGCTTCTCTTCCAGGTAAGCAATCAGGTCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCcccc                                                                                                                                      >  1:380295/1‑129 (MQ=255)            cgccAGATGTTCCGCCATTTCCGGCTTCTCTTCCAGGTAAGCAATCAGGTCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCcccc                                                                                                                                      <  2:380295/129‑1 (MQ=255)            cgccAGATGTTCCGCCATTTCCGGCTTCTCTTCCAGGTAAGCAATCAGGTCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCCCCCGCGTTCCTGAc                                                                                                                           >  1:185069/1‑140 (MQ=255)                            aTTTCCGGCTTCTCTTCCAGGTAAGCAATCAGGTCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCCCCCGCGTTCCTGACGATCGAGCGAAATCaa                                                                                                           <  1:763261/140‑1 (MQ=255)                             tttCCGGCTTCTCTTCCAGGTAAGCAATCAGGTCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCCCCCGCGTTCCTGACGATCGAGCGAAATCAac                                                                                                          <  2:253262/140‑1 (MQ=255)                              ttCCGGCTTCTCTTCCAGGTAAGCAATCAGGTCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCCCCCGCGTTCCTGACGATCGAGCGAAATCAaca                                                                                                         <  1:866236/140‑1 (MQ=255)                               tCCGGCTTCTCTTCCAGGTAAGCAATCAGGTCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCCCCCGCGTTCCTGACGATCGAGCGAAATCAacac                                                                                                        >  2:911442/1‑140 (MQ=255)                                ccGGCTTCTCTTCCAGGTAAGCAATCAGGTCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCCCCCGCGTTCCTGACGATCGAGCGAAATCAACACg                                                                                                       <  1:1370225/140‑1 (MQ=255)                                ccGGCTTCTCTTCCAGGTAAGCAATCAGGTCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCCCCCGCGTTCCTGACGATCGAGCGAAATCAACACg                                                                                                       <  1:911442/140‑1 (MQ=255)                                          ttCCAGGTAAGCAATCAGGTCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCCCCCGCGTTCCTGACGATCGAGCGAAATCAACACGCCAGCAAGCg                                                                                             >  1:840582/1‑140 (MQ=255)                                               ggTAAGCAATCAGGTCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCCCCCGCGTTCCTGACGATCGAGCGAAATCAACACGCCAGCAAGCGTcgcg                                                                                        >  1:1350122/1‑140 (MQ=255)                                                  aaGCAATCAGGTCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCCCCCGCGTTCCTGACGATCGAGCGAAATCAACACGCCAGCAAGCGTCGCGCCa                                                                                     >  1:599269/1‑140 (MQ=255)                                                  aaGCAATCAGGTCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCCCCCGCGTTCCTGACGATCGAGCGAAATCAACACGCCAGCAAGCGTCGCGCCa                                                                                     >  1:1058374/1‑140 (MQ=255)                                                  aaGCAATCAGGTCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCCCCCGCGTTCCTGACGATCGAGCGAAATCAACACGCCAGCAAGCGTCGCGCCa                                                                                     >  1:780808/1‑140 (MQ=255)                                                  aaGCAATCAGGTCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCCCCCGCGTTCCTGACGATCGAGCGAAATCAACACGCCAGCAAGCGTCGCGCCa                                                                                     <  1:1300995/140‑1 (MQ=255)                                                  aaGCAATCAGGTCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCCCCCGCGTTCCTGACGATCGAGCGAAATCAACACGCCAGCAAGCGTCGCGCCa                                                                                     <  2:606280/140‑1 (MQ=255)                                                     cAATCAGGTCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCCCCCGCGTTCCTGACGATCGAGCGAAATCAACACGCCAGCAAGCGTCGCGCCATTg                                                                                  >  1:721210/1‑140 (MQ=255)                                                       aTCAGGTCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCCCCCGCGTTCCTGACGATCGAGCGAAATCAACACGCCAGCAAGCGTCGCGCCATTGGc                                                                                <  2:378544/140‑1 (MQ=255)                                                       aTCAGGTCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCCCCCGCGTTCCTGACGATCGAGCGAAATCAACACGCCAGCAAGCGTCGCGCCATTGGc                                                                                <  2:185069/140‑1 (MQ=255)                                                        tCAGGTCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCCCCCGCGTTCCTGACGATCGAGCGAAATCAACACGCCAGCAAGCGTCGCGCCATTGGcc                                                                               <  2:1058374/140‑1 (MQ=255)                                                        tCAGGTCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCCCCCGCGTTCCTGACGATCGAGCGAAATCAACACGCCAGCAAGCGTCGCGCCATTGGcc                                                                               <  2:721210/140‑1 (MQ=255)                                                        tCAGGTCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCCCCCGCGTTCCTGACGATCGAGCGAAATCAACACGCCAGCAAGCGTCGCGCCATTGGcc                                                                               <  2:599269/140‑1 (MQ=255)                                                          aGGTCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCCCCCGCGTTCCTGACGATCGAGCGAAATCAACACGCCAGCAAGCGTCGCGCCATTGGc                                                                                <  1:712589/137‑1 (MQ=255)                                                          aGGTCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCCCCCGCGTTCCTGACGATCGAGCGAAATCAACACGCCAGCAAGCGTCGCGCCATTGGc                                                                                >  2:712589/1‑137 (MQ=255)                                                            gTCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCCCCCGCGTTCCTGACGATCGAGCGAAATCAACACGCCAGCAAGCGTCGCGCCATTGGCCTGaa                                                                           >  1:552701/1‑140 (MQ=255)                                                               tttCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCCCCCGCGTTCCTGACGATCGAGCGAAAT‑AACACGCCAGCAAGCGTCGCGCCATTGGCCTGaataat                                                                       <  2:1524985/140‑1 (MQ=255)                                                                ttCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCCCCCGCGTTCCTGACGATCGAGCGAAATCAACACGCCAGCAAGCGTCGCGCCATTGGCCTGaataat                                                                       <  1:61471/140‑1 (MQ=255)                                                                  cAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCCCCCGCGTTCCTGACGATCGAGCGAAATCAACACGCCAGCAAGCGTCGCGCCATTGGCCTGaataat                                                                       >  2:665963/1‑138 (MQ=255)                                                                  cAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCCCCCGCGTTCCTGACGATCGAGCGAAATCAACACGCCAGCAAGCGTCGCGCCATTGGCCTGaataat                                                                       <  1:665963/138‑1 (MQ=255)                                                                      gTGATGATAGAGATCACTTTGCAGTTGTAATCACTCTCAACTTCCTGAATCGCCGAAATCTCGCCGCCCCCGCGTTCCTGACGATCGAGCGAAATCAACACGCCAGCAAGCGTCGCGCCATTGGCCTGAATAATCTCCAt                                                                 >  2:167772/1‑140 (MQ=255)                                                                      gTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCCCCCGCGTTCCTGACGATCGAGCGAAATCAACACGCCAGCAAGCGTCGCGCCATTGGCCTGAATAATCTCCAt                                                                 >  1:1556851/1‑140 (MQ=255)                                                                      gTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCCCCCGCGTTCCTGACGATCGAGCGAAATCAACACGCCAGCAAGCGTCGCGCCATTGGCCTGAATAATCTCCAt                                                                 >  1:479806/1‑140 (MQ=255)                                                                         atgatAGGGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCCCCCGCGTTCCTGACGATCGAGCGAAATCAACACGCCAGCAAGCGTCGCGCCATTGGCCTGAATAATCTCCATCGa                                                              >  1:355535/1‑140 (MQ=255)                                                                         atgatAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCCCCCGCGTTCCTGACGATCGAGCGAAATCAACACGCCAGCAAGCGTCGCGCCATTGGCCTGAATAATCTCCATCGa                                                              >  1:1575302/1‑140 (MQ=255)                                                                               gagaTCACTTTGCAGTTGTAATCACTCTCAACTTCCTGAATCGCCGAAATCTCGCCGCCCCCGCGTTCCTGACGATCGAGCGAAATCAACACGCCAGCAAGCGTCGCGCCATTGGCCTGAATAATCTCCATCGACTcgcg                                                        <  1:167772/140‑1 (MQ=255)                                                                                agaTCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCCCCCGCGTTCCTGACGATCGAGCGAAATCAACACGCCAGCAAGCGTCGCGCCATTGGCCTGAATAATCTCCATCGACTCGCGa                                                       >  1:193978/1‑140 (MQ=255)                                                                                 gaTCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCCCCCGCGTTCCTGACGATCGAGCGAAATCAACACGCCAGCAAGCGTCGCGCCATTGGCCTGAATAATCTCCATCGACTCGCGaa                                                      >  1:209302/1‑140 (MQ=255)                                                                                 gaTCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCCCCCGCGTTCCTGACGATCGAGCGAAATCAACACGCCAGCAAGCGTCGCGCCATTGGCCTGAATAATCTCCATCGACTCGCGaa                                                      >  1:1224966/1‑140 (MQ=255)                                                                                  aTCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCCCCCGCGTTCCTGACGATCGAGCGAAATCAACACGCCAGCAAGCGTCGCGCCATTGGCCTGAATAATCTCCATCGACTCGCGAAt                                                     >  1:211111/1‑140 (MQ=255)                                                                                   tCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCCCCCGCGTTCCTGACGATCGAGCGAAATCAACACGCCAGCAAGCGTCGCGCCATTGGCCTGAATAATCTCCATCGACTCGCGAATc                                                    >  2:1343093/1‑140 (MQ=255)                                                                                   tCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCCCCCGCGTTCCTGACGATCGAGCGAAATCAACACGCCAGCAAGCGTCGCGCCATTGGCCTGAATAATCTCCATCGACTCGCGAATc                                                    >  1:1149249/1‑140 (MQ=255)                                                                                   tCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCCCCCGCGTTCCTGACGATCGAGCGAAATCAACACGCCAGCAAGCGTCGCGCCATTGGCCTGAATAATCTCCATCGACTCGCGAATc                                                    >  2:1244881/1‑140 (MQ=255)                                                                                    cACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCCCCCGCGTTCCTGACGATCGAGCGAAATCAACACGCCAGCAAGCGTCGCGCCATTGGCCTGAATAATCTCCATCGACTCGCGAATCg                                                   <  2:1224966/140‑1 (MQ=255)                                                                                    cACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCCCCCGCGTTCCTGACGATCGAGCGAAATCAACACGCCAGCAAGCGTCGCGCCATTGGCCTGAATAATCTCCATCGACTCGCGAATCg                                                   >  1:417718/1‑140 (MQ=255)                                                                                    cACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCCCCCGCGTTCCTGACGATCGAGCGAAATCAACACGCCAGCAAGCGTCGCGCCATTGGCCTGAATAATCTCCATCGACTCGCGAATCg                                                   >  2:323808/1‑140 (MQ=255)                                                                                    cACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCCCCCGCGTTCCTGACGATCGAGCGAAATCAACACGCCAGCAAGCGTCGCGCCATTGGCCTGAATAATCTCCATCGACTCGCGAATCg                                                   >  1:772234/1‑140 (MQ=255)                                                                                    cACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCCCCCGCGTTCCTGACGATCGAGCGAAATCAACACGCCAGCAAGCGTCGCGCCATTGGCCTGAATAATCTCCATCGACTCGCGAATCg                                                   >  1:859772/1‑140 (MQ=255)                                                                                     aCTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCCCCCGCGTTCCTGACGATCGAGCGAAATCAACACGCCAGCAAGCGTCGCGCCATTGGCCTGAATAATCTCCATCGACTCGCGAATCGc                                                  >  1:1262128/1‑140 (MQ=255)                                                                                       tttGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCCCCCGCGTTCCTGACGATCGAGCGAAATCAACACGCCAGCAAGCGTCGCGCCATTGGCCTGAATAATCTCCATCGACTCGCGAATCGCCg                                                <  2:1149249/140‑1 (MQ=255)                                                                                         tGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCCCCCGCGTTCCTGACGATCGAGCGAAATCAACACGCCAGCAAGCGTCGCGCCATTGGCCTGAATAATCTCCATCGACTCGCGAATCGCCGtt                                              >  1:1170638/1‑140 (MQ=255)                                                                                         tGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCCCCCGCGTTCCTGACGATCGAGCGAAATCAACACGCCAGCAAGCGTCGCGCCATTGGCCTGAATAATCTCCATCGACTCGCGAATCGCCGtt                                              >  1:976847/1‑140 (MQ=255)                                                                                          gCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCCCCCGCGTTCCTGACGATCGAGCGAAATCAACACGCCAGCAAGCGTCGCGCCATTGGCCTGAATAATCTCCATCGACTCGCGAATCGCCGTTc                                             <  1:170470/140‑1 (MQ=255)                                                                                            aGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCCCCCGCGTTCCTGACGATCGAGCGAAATCAACACGCCAGCAAGCGTCGCGCCATTGGCCTGAATAATCTCCATCGACTCGCGAATCGCCGTTCcg                                           <  2:840582/140‑1 (MQ=255)                                                                                             gTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCCCCCGCGTTCCTGACGATCGAGCGAAATCAACACGCCAGCAAGCGTCGCGCCATTGGCCTGAATAATCTCCATCGACTCGCGAATCGCCGTTCcgg                                          <  2:193978/140‑1 (MQ=255)                                                                                              ttGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCCCCCGCGTTCCTGACGATCGAGCGAAATCAACACGCCAGCAAGCGTCGCGCCATTGGCCTGAATAATCTCCATCGACTCGCGAATCGCCGTTCcggc                                         <  2:611058/140‑1 (MQ=255)                                                                                               tGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCCCCCGCGTTCCTGACGATCGAGCGAAATCAACACGCCAGCAAGCGTCGCGCCATTGGCCTGAATAATCTCCATCGACTCGCGAATCGCCGTTCcggcg                                        <  2:780808/140‑1 (MQ=255)                                                                                                 tAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCCCCCGCGTTCCTGACGATCGAGCGAAATCAACACGCCAGCAAGCGTCGCGCCATTGGCCTGAATAATCTCCATCGACTCGTGAATCGCCGTTCCGGCGGt                                      <  1:1191749/140‑1 (MQ=255)                                                                                                 tAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCCCCCGCGTTCCTGACGATCGAGCGAAATCAACACGCCAGCAAGCGTCGCGCCATTGGCCTGAATAATCTCCATCGACTCGCGAATCGCCGTTCcggcgg                                       <  1:673400/139‑1 (MQ=255)                                                                                                 tAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCCCCCGCGTTCCTGACGATCGAGCGAAATCAACACGCCAGCAAGCGTCGCGCCATTGGCCTGAATAATCTCCATCGACTCGCGAATCGCCGTTCcggcgg                                       >  2:673400/1‑139 (MQ=255)                                                                                                   aTCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCCCCCGCGTTCCTGACGATCGAGCGAAATCAACACGCCAGCAAGCGTCGCGCCATTGGCCTGAATAATCTCCATCGACTCGCGAATCGCCGTTCCGGCGGTGa                                    >  1:898511/1‑140 (MQ=255)                                                                                                      aCGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCCCCCGCGTTCCTGACGATCGAGCGAAATCAACACGCCAGCAAGCGTCGCGCCATTGGCCTGAATAATCTCCATCGACTCGCGAATCGCCGTTCCGGCGGTGATca                                 <  2:1262128/140‑1 (MQ=255)                                                                                                        gCTCAACTTCCTGAATCGCCGAAATCTCGCCGCCCCCGCGTTCCTGACGATCGAGCGAAATCAACACGCCAGCAAGCGTCGCGCCATTGGCCTGAATAATCTCCATCGACTCGCGAATCGCCGTTCCGGCGGTGATcaca                               >  2:1419908/1‑140 (MQ=255)                                                                                                        gCTCAACTTCCTGAATCGCCGAAATCTCGCCGCCCCCGCGTTCCTGACGATCGAGCGAAATCAACACGCCAGCAAGCGTCGCGCCATTGGCCTGAATAATCTCCATCGACTCGCGAATCGCCGTTCCGGCGGTGATcaca                               >  1:73871/1‑140 (MQ=255)                                                                                                        gCTCAACTTCCTGAATCGCCGAAATCTCGCCGCCCCCGCGTTCCTGACGATCGAGCGAAATCAACACGCCAGCAAGCGTCGCGCCATTGGCCTGAATAATCTCCATCGACTCGCGAATCGCCGTTCCGGCGGTGATcaca                               >  1:586697/1‑140 (MQ=255)                                                                                                        gCTCAACTTCCTGAATCGCCGAAATCTCGCCGCCCCCGCGTTCCTGACGATCGAGCGAAATCAACACGCCAGCAAGCGTCGCGCCATTGGCCTGAATAATCTCCATCGACTCGCGAATCGCCGTTCCGGCGGTGATcaca                               >  1:26969/1‑140 (MQ=255)                                                                                                          tCAACTTCCTGAATCGCCGAAATCTCGCCGCCCCCGCGTTCCTGACGATCGAGCGAAATCAACACGCCAGCAAGCGTCGCGCCATTGGCCTGAATAATCTCCATCGACTCGCGAATCGCCGTTCCGGCGGTGATCAcatc                             >  1:88254/1‑140 (MQ=255)                                                                                                           cAACTTCCTGAATCGCCGAAATCTCGCCGCCCCCGCGTTCCTGACGATCGAGCGAAATCAACACGCCAGCAAGCGTCGCGCCATTGGCCTGAATAATCTCCATCGACTCGCGAATCGCCGTTCCGGCGGTGATCAcatca                            >  1:1339545/1‑140 (MQ=255)                                                                                                           cAACTTCCTGAATCGCCGAAATCTCGCCGCCCCCGCGTTCCTGACGATCGAGCGAAATCAACACGCCAGCAAGCGTCGCGCCATTGGCCTGAATAATCTCCATCGACTCGCGAATCGCCGTTCCGGCGGTGATCAcatca                            <  2:859772/140‑1 (MQ=255)                                                                                                           cAACTTCCTGAATCGCCGAAATCTCGCCGCCCCCGCGTTCCTGACGATCGACCGAAATCAACACGCCAGCAAGCGTCGCGCCATTGGCCTGAATAATCTCCATCGACTCGCGAATCGCCGTTCCGGCGGTGATCAcatca                            <  2:355535/140‑1 (MQ=255)                                                                                                           cAACTTCCTGAATCGCCGAAATCTCGCCGCCACCGCGTTCCTGACGATCGAGCGAAATCAACACGCCAGCAAGCGTCGCGCCATTGGCCTGAATAATCTCCATCGACTCGCGAATCGCCGTTCCGGCGGTGATCAcatca                            >  1:530861/1‑140 (MQ=255)                                                                                                              cTTCCTGAATCGCCGAAATCTCGCCGCCCCCGCGTTCCTGACGATCGAGCGAAATCAACACGCCAGCAAGCGTCGCGCCATTGGCCTGAATAATCTCCATCGACTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCt                         >  2:897455/1‑140 (MQ=255)                                                                                                                tCCTGAATCGCCGAAATCTCGCCGCCCCCGCGTTCCTGACGATCGAGCGAAATCAACACGCCAGCAAGCGTCGCGCCATTGGCCTGAATAATCTCCATCGACTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTAc                       <  2:552701/140‑1 (MQ=255)                                                                                                                      aTCGCCGAAATCTCGCCGCCCCCGCGTTCCTGACGATCGAGCGAAATCAACACGCCAGCAAGCGTCGCGCCATTGGCCTGAATAATCTCCATCGACTCGCGAATCTCCGTTCCGGCGGTGATCACATCATCTACCAGCAt                 >  2:381849/1‑140 (MQ=255)                                                                                                                          ccGAAATCTCGCCGCCCCCGCGTTCCTGACGATCGAGCGAAATCAACACGCCAGCAAGCGTCGCGCCATTGGCCTGAATAATCTCCATCGACTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTAcg             <  1:323808/140‑1 (MQ=255)                                                                                                                          ccGAAATCTCGCCGCCCCCGCGTTCCTGACGATCGAGCGAAATCAACACGCCAGCAAGCGTCGCGCCATTGGCCTGAATAATCTCCATCGACTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTAcg             <  1:1343093/140‑1 (MQ=255)                                                                                                                          ccGAAATCTCGCCGCCCCCGCGTTCCTGACGATCGAGCGAAATCAACACGCCAGCAAGCGTCGCGCCATTGGCCTGAATAATCTCCATCGACTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTAcg             <  1:381849/140‑1 (MQ=255)                                                                                                                          ccGAAATCTCGCCGCCCCCGCGTTCCTGACGATCGAGCGAAATCAACACGCCAGCAAGCGTCGCGCCATTGGCCTGAATAATCTCCATCGACTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTAcg             <  2:772234/140‑1 (MQ=255)                                                                                                                          ccGAAATCTCGCCGCCCCCGCGTTCCTGACGATCGAGCGAAATCAACACGCCAGCAAGCGTCGCGCCATTGGCCTGAATAATCTCCATCGACTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTAcg             <  2:1339545/140‑1 (MQ=255)                                                                                                                          ccGAAATCTCGCCGCCCCCGCGTTCCTGACGATCGAGCGAAATCAACACGCCAGCAAGCGTCGCGCCATTGGCCTGAATAATCTCCATCGACTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTAcg             <  2:26969/140‑1 (MQ=255)                                                                                                                          ccGAAATCTCGCCGCCCCCGCGTTCCTGACGATCGAGCGAAATCAACACGCCAGCAAGCGTCGCGCCATTGGCCTGAATAATCTCCATCGACTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTAcg             <  2:209302/140‑1 (MQ=255)                                                                                                                          ccGAAATCTCGCCGCCACCGCGTTCCTGACGATCGAGCGAAATCAACACGCCAGCAAGCGTCGCGCCATTGGCCTGAATAATCTCCATCGACTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTAcg             <  2:530861/140‑1 (MQ=255)                                                                                                                                     ccgcCCCCGCGTTCCTGACGATCGAGCGAAATCAACACGCCAGCAAGCGTCGCGCCATTGGCCTGAATAATCTCCATCGACTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTaa  >  1:237403/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                        GCCTTAACCGCCGCCAGATGTTCCGCCATTTCCGGCTTCTCTTCCAGGTAAGCAATCAGGTCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCACGCTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCGCGTTCCTGACGATCGAGCGAAATCAACACGCCAGCAAGCGTCGCGCCATTGGCCTGAATAATCTCCATCGACTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAA  >  NC_000913/3815152‑3815423

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑