Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	757,240	A→T	N445I (AAC→ATC)	sdhA →	succinate:quinone oxidoreductase, FAD binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	757,240	0	A	T	100.0%	260.8 / NA	76	N445I (AAC→ATC)	sdhA	succinate:quinone oxidoreductase, FAD binding protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (29/47);  total (29/47)

CTGGGCGGCAACTCGCTGCTGGACCTGGTGGTCTTTGGTCGCGCGGCAGGTCTGCATCTGCAAGAGTCTATCGCCGAGCAGGGCGCACTGCGCGATGCCAGCGAGTCTGATGTTGAAGCGTCTCTGGATCGCCTGAACCGCTGGAACAATAATCGTAACGGTGAAGATCCGGTGGCGATCCGTAAAGCGCTGCAAGAATGTATGCAGCATAACTTCTCGGTCTTCCGTGAAGGTGATGCGATGGCGAAAGGGCTTGAGCAGTTGAAAGTGA  >  NC_000913/757104‑757374                                                                                                                                         |                                                                                                                                       cTGGGCGGCAACTCGCTGCTGGACCTGGTGGTCTTTGGTCGCGCGGCAGGTCTGCATCTGCAAGAGTCTATCGCCGAGCAGGGCGCACTGCGCGATGCCAGCGAGTCTGATGTTGAAGCGTCTCTGGATCGCCTGATCCg                                                                                                                                     >  1:391307/1‑140 (MQ=255) cTGGGCGGCAACTCGCTGCTGGACCTGGTGGTCTTTGGTCGCGCGGCAGGTCTGCATCTGCAAGAGTCTATCGCCGAGCAGGGCGCACTGCGCGATGCCAGCGAGTCTGATGTTGAAGCGTCTCTGGATCGCCTGATCCg                                                                                                                                     >  2:514792/1‑140 (MQ=255) cTGGGCGGCAACTCGCTGCTGGACCTGGTGGTCTTTGGTCGCGCGGCAGGTCTGCATCTGCAAGAGTCTATCGCCGAGCAGGGCGCACTGCGCGATGCCAGCGAGTCTGATGTTGAAGCGTCTCTGGATCGCCTGATCCg                                                                                                                                     >  1:987592/1‑140 (MQ=255)  tGGGCGGCAACTCGCTGCTGGACCTGGTGGTCTTTGGTCGCGCGGCAGGTCTGCATCTGCAAGAGTCTATCGCCGAGCAGGGCGTACTGCGCGATGCCAGCGAGTCTGATGTTGAAGCGTCTCTGGATCGCCTGATCCGc                                                                                                                                    <  1:1342146/140‑1 (MQ=255)  tGGGCGGCAACTCGCTGCTGGACCTGGTGGTCTTTGGTCGCGCGGCAGGTCTGCATCTGCAAGAGTCTATCGCCGAGCAGGGCGCACTGCGCGATGCCAGCGAGTCTGATGTTGAAGCGTCTCTGGATCGCCTGATCCGc                                                                                                                                    <  2:406111/140‑1 (MQ=255)  tGGGCGGCAACTCGCTGCTGGACCTGGTGGTCTTTGGTCGCGCGGCAGGTCTGCATCTGCAAGAGTCTATCGCCGAGCAGGGCGCACTGCGCGATGCCAGCGAGTCTGATGTTGAAGCGTCTCTGGATCGCCTGATCCGc                                                                                                                                    <  1:1092090/140‑1 (MQ=255)     gcggcAACTCGCTGCTGGACCTGGTGGTCTTTGGTCGCGCGGCAGGTCTGCATCTGCAAGAGTCTATCGCCGAGCAGGGCGCACTGCGCGATGCCAGCGAGTCTGATGTTGAAGCGTCTCTGGATCGCCTGATCCGCTgg                                                                                                                                 <  1:1260501/140‑1 (MQ=255)      cggcAACTCGCTGCTGGACCTGGTGGTCTTTGGTCGCGCGGCAGGTCTGCATCTGCAAGAGTCTATCGCCGAGCAGGGCGCACTGCGCGATGCCAGCGAGTCTGATGTTGAAGCGTCTCTGGATCGCCTGATCCg                                                                                                                                     >  2:1526673/1‑135 (MQ=255)      cggcAACTCGCTGCTGGACCTGGTGGTCTTTGGTCGCGCGGCAGGTCTGCATCTGCAAGAGTCTATCGCCGAGCAGGGCGCACTGCGCGATGCCAGCGAGTCTGATGTTGAAGCGTCTCTGGATCGCCTGATCCg                                                                                                                                     <  1:1526673/135‑1 (MQ=255)      cggcAACTCGCTGCTGGACCTGGTGGTCTTTGGTCGCGCGGCAGGTCTGCATCTGCAAGAGTCTATCGCCGAGCAGGGCGCACTGCGCGATGCCAGCGAGTCTGATGTTGAAGCGTCTCTGGATCGCCTGATCCGCTGGa                                                                                                                                >  1:965289/1‑140 (MQ=255)      cggcAACTCGCTGCTGGACCTGGTGGTCTTTGGTCGCGCGGCAGGTCTGCATCTGCAAGAGTCTATCGCCGAGCAGGGCGCACTGCGCGATGCCAGCGAGTCTGATGTTGAAGCGTCTCTGGATCGCCTGATCCGCTGGa                                                                                                                                >  2:300886/1‑140 (MQ=255)              cGCTGCTGGACCTGGTGGTCTTTGGTCGCGCGGCAGGTCTGCATCTGCAAGAGTCTATCGCCGAGCAGGGCGCACTGCGCGATGCCAGCGAGTCTGATGTTGAAGCGTCTCTGGATCGCCTGATCCGCTGGAACaataat                                                                                                                        >  2:881477/1‑140 (MQ=255)                 tgctgGACCTGGTGGTCTTTGGTCGCGCGGCAGGTCTGCATCTGCAAGAGTCTATCGCCGAGCAGGGCGCACTGCGCGATGCCAGCGAGTCTGATGTTGAAGCGTCTCTGGATCGCCTGATCCGCTGGAACAATAATCGt                                                                                                                     <  1:912469/140‑1 (MQ=255)                  gctgGACCTGGTGGTCTTTGGTCGCGCGGCAGGTCTGCATCTGCAAGAGTCTATCGCCGAGCAGGGCGCACTGCGCGATGCCAGCGAGTCTGATGTTGAAGCGTCTCTGGATCGCCTGATCCGCTGGAACAATAATCGTa                                                                                                                    <  1:833924/140‑1 (MQ=255)                   ctgGACCTGGTGGTCTTTGGTCGCGCGGCAGGTCTGCATCTGCAAGAGTCTATCGCCGAGCAGGGCGCACTGCGCGATGCCAGCGAGTCTGATGTTGAAGCGTCTCTGGATCGCCTGATCCGCTGGAACAATAATCGTaa                                                                                                                   <  1:344923/140‑1 (MQ=255)                   ctgGACCTGGTGGTCTTTGGTCGCGCGGCAGGTCTGCATCTGCAAGAGTCTATCGCCGAGCAGGGCGCACTGCGCGATGCCAGCGAGTCTGATGTTGAAGCGTCTCTGGATCGCCTGATCCGCTGGAACAATAATCGTaa                                                                                                                   <  1:769588/140‑1 (MQ=255)                    tgGACCTGGTGGTCTTTGGTCGCGCGGCAGGTCTGCATCTGCAAGAGTCTATCGCCGAGCAGGGCGCACTGCGCGATGCCAGCGAGTCTGATGTTGAAGCGTCTCTGGATCGCCTGATCCGCTGGAACAATAATCGTAAc                                                                                                                  <  2:1507788/140‑1 (MQ=255)                      gACCTGGTGGTCTTTGGTCGCGCGGCAGGTCTGCATCTGCAAGAGTCTATCGCCGAGCAGGGCGCACTGCGCGATGCCAGCGAGTCTGATGTTGAAGCGTCTCTGGATCGCCTGATCCGCTGGAACAATAATCGTAACgg                                                                                                                <  2:134614/140‑1 (MQ=255)                       aCCTGGTGGTCTTTGGTCGCGCGGCAGGTCTGCATCTGCAAGAGTCTATCGCCGAGCAGGGCGCACTGCGCGATGCCAGCGAGTCTGATGTTGAAGCGTCTCTGGATCGCCTGATCCGCTGGAACAATAATCGTAACGGt                                                                                                               <  1:1495433/140‑1 (MQ=255)                       aCCTGGTGGTCTTTGGTCGCGCGGCAGGTCTGCATCTGCAAGAGTCTATCGCCGAGCAGGGCGCACTGCGCGATGCCAGCGAGTCTGATGTTGAAGCGTCTCTGGATCGCCTGATCCGCTGGAACAATAATCGTAACGGt                                                                                                               <  2:347929/140‑1 (MQ=255)                         cTGGTGGTCTTTGGTCGCGCGGCAGGTCTGCATCTGCAAGAGTCTATCGCCGAGCAGGGCGCACTGCGCGATGCCAGCGAGTCTGATGTTGAAGCGTCTCTGGATCGCCTGATCCGCTGGAACAATAATCGTAACGGTGa                                                                                                             <  2:1568321/140‑1 (MQ=255)                         cTGGTGGTCTTTGGTCGCGCGGCAGGTCTGCATCTGCAAGAGTCTATCGCCGAGCAGGGCGCACTGCGCGATGCCAGCGAGTCTGATGTTGAAGCGTCTCTGGATCGCCTGATCCGCTGGAACAATAATCGTAACGGTGa                                                                                                             <  1:278933/140‑1 (MQ=255)                         cTGGTGGTCTTTGGTCGCGCGGCAGGTCTGCATCTGCAAGAGTCTATCGCCGAGCAGGGCGCACTGCGCGATGCCAGCGAGTCTGATGTTGAAGCGTCTCTGGATCGCCTGATCCGCTGGAACAATAATCGTAACGGTGa                                                                                                             >  2:318458/1‑140 (MQ=255)                           ggtggtCTTTGGTCGCGCGGCAGGTCTGCATCTGCAAGAGTCTATCGCCGAGCAGGGCGCACTGCGCGATGCCAGCGAGTCTGATGTTGAAGCGTCTCTGGATCGCCTGATCCGCTGGAACAATAATCGTAACGGTGAAg                                                                                                           >  1:1036374/1‑140 (MQ=255)                            gtggtCTTTGGTCGCGCGGCAGGTCTGCATCTGCAAGAGTCTATCGCCGAGCAGGGCGCACTGCGCGATGCCAGCGAGTCTGATGTTGAAGCGTCTCTGGATCGCCTGATCCGCTGGAACAATAATCGTAACGGTGAAGa                                                                                                          <  2:1368480/140‑1 (MQ=255)                                 cTTTGGTCGCGCGGCAGGTCTGCATCTGCAAGAGTCTATCGCCGAGCAGGGCGCACTGCGCGATGCCAGCGAGTCTGATGTTGAAGCGTCTCTGGATCGCCTGATCCGCTGGAACAATAATCGTAACGGTGAAGATCCgg                                                                                                     <  1:318458/140‑1 (MQ=255)                                  tttGGTCGCGCGGCAGGTCTGCATCTGCAAGAGTCTATCGCCGAGCAGGGCGCACTGCGCGATGCCAGCGAGTCTGATGTTGAAGCGTCTCTGGATCGCCTGATCCGCTGGAACAATAATCGTaa                                                                                                                   <  1:599497/125‑1 (MQ=255)                                  tttGGTCGCGCGGCAGGTCTGCATCTGCAAGAGTCTATCGCCGAGCAGGGCGCACTGCGCGATGCCAGCGAGTCTGATGTTGAAGCGTCTCTGGATCGCCTGATCCGCTGGAACAATAATCGTaa                                                                                                                   >  2:599497/1‑125 (MQ=255)                                   ttGGTCGCGCGGCAGGTCTGCATCTGCAAGAGTCTATCGCCGAGCAGGGCGCACTGCGCGATGCCAGCGAGTCTGATGTTGAAGCGTCTCTGGATCGCCTGATCCGCTGGAACAATAATCGTAACGGTGAAGATCCGGTg                                                                                                   <  1:1183166/140‑1 (MQ=255)                                     ggTCGCGCGGCAGGTCTGCATCTGCAAGAGTCTATCGCCGAGCAGGGCGCACTGCGCGATGCCAGCGAGTCTGATGTTGAAGCGTCTCTGGATCGCCTGATCCGCTGGAACAATAATCGTAACGGTGAAGATCCGGTGGc                                                                                                 <  2:391307/140‑1 (MQ=255)                                      gTCGCGCGGCAGGTCTGCATCTGCAAGAGTCTATCGCCGAGCAGGGCGCACTGCGCGATGCCAGCGAGTCTGATGTTGAAGCGTCTCTGGATCGCCTGATCCGCTGGAACAATAATCGTAACGGTGAAGATCCGGTGGCg                                                                                                <  1:435093/140‑1 (MQ=255)                                      gTCGCGCGGCAGGTCTGCATCTGCAAGAGTCTATCGCCGAGCAGGGCGCACTGCGCGATGCCAGCGAGTCTGATGTTGAAGCGTCTCTGGATCGCCTGATCCGCTGGAACAATAATCGTAACGGTGAAGATCCGGTGGCg                                                                                                <  1:881477/140‑1 (MQ=255)                                      gTCGCGCGGCAGGTCTGCATCTGCAAGAGTCTATCGCCGAGCAGGGCGCACTGCGCGATGCCAGCGAGTCTGATGTTGAAGCGTCTCTGGATCGCCTGATCCGCTGGAACAATAATCGTAACGGTGAAGATCCGGTGGCg                                                                                                <  2:309236/140‑1 (MQ=255)                                           gcgGCAGGTCTGCATCTGCAAGAGTCTATCGCCGAGCAGGGCGCACTGCGCGATGCCAGCGAGTCTGATGTTGAAGCGTCTCTGGATCGCCTGATCCGCTGGAACAATAATCGTAACGGTGAAGATCCGGTGGCGATCCg                                                                                           >  2:359427/1‑140 (MQ=255)                                            cgGCAGGTCTGCATCTGCAAGAGTCTATCGCCGAGCAGGGCGCACTGCGCGATGCCAGTGAGTCTGATGTTGAAGCGTCTCTGGATCGCCTGATCCGCTGGAACAATAATCGTAACGGTGAAGATCCGGTGGCGATCCGt                                                                                          >  2:1076012/1‑140 (MQ=255)                                                aGGTCTGCATCTGCAAGAGTCTATCGCCGAGCAGGGCGCACTGCGCGATGCCAGCGAGTCTGATGTTGAAGCGTCTCTGGATCGCCTGATCCGCTGGAACAATAATCGTAACGGTGAAGATCCGGTGGCGATCCGTAAAg                                                                                      <  1:347940/140‑1 (MQ=255)                                                  gTCTGCATCTGCAAGAGTCTATCGCCGAGCAGGGCGCACTGCGCGATGCCAGTGAGTCTGATGTTGAAGCGTCTCTGGATCGCCTGATCCGCTGGAACAATAATCGTAACGGTGAAGATCCGGTGGCGATCCGTAAAgcg                                                                                    <  1:1076012/140‑1 (MQ=255)                                                       catctgcaAGAGTCTATCGCCGAGCAGGGCGCACTGCGCGATGCCAGCGAGTCTGATGTTGAAGCGTCTCTGGATCGCCTGATCCGCTGGAACAATAATCGTAACGGTGAAGATCCGGTGGCGATCCGTAAAGCGCTGCa                                                                               <  1:359427/140‑1 (MQ=255)                                                       catctgcaAGAGTCTATCGCCGAGCAGGGCGCACTGCGCGATGCCAGCGAGTCTGATGTTGAAGCGTCTCTGGATCGCCTGATCCGCTGGAACAATAATCGTAACGGTGAAGATCCGGTGGCGATCCGTAAAGCGCTGCa                                                                               <  2:1016979/140‑1 (MQ=255)                                                       catctgcaAGAGTCTATCGCCGAGCAGGGCGCACTGCGCGATGCCAGCGAGTCTGATGTTGAAGCGTCTCTGGATCGCCTGATCCGCTGGAACAATAATCGTAACGGTGAAGATCCGGTGGCGATCCGTAAAGCGCTGCa                                                                               <  1:569938/140‑1 (MQ=255)                                                            gcaAGAGTCTATCGCCGAGCAGGGCGCACTGCGCGATGCCAGCGAGTCTGATGTTGAAGCGTCTCTGGATCGCCTGATCCGCTGGAACAATAATCGTAACGGTGAAGATCCGGTGGCGATCCGTAAAGCGCTGCAAGAAt                                                                          >  1:1389755/1‑140 (MQ=255)                                                                 agTCTATCGCCGAGCAGGGCGCACTGCGCGATGCCAGCGAGTCTGATGTTGAAGCGTCTCTGGATCGCCTGATCCGCTGGAACAATAATCGTAACGGTGAAGATCCGGTGGCGATCCGTAAAGCGCTGCAAGAATGTATg                                                                     <  2:1389755/140‑1 (MQ=255)                                                                  gTCTATCGCCGAGCAGGGCGCACTGCGCGATGCCAGCGAGTCTGATGTTGAAGCGTCTCTGGATCGCCTGATCCGCTGGAACAATAATCGTAACGGTGAAGATCCGGTGGCGATCCGTAAAGCGCTGCAAGAATGTATgc                                                                    <  2:802237/140‑1 (MQ=255)                                                                       tCGCCGAGCAGGGCGCACTGCGCGATGCCAGCGAGTCTGATGTTGAAGCGTCTCTGGATCGCCTGATCCGCTGGAACAATAATCGTAACGGTGAAGATCCGGTGGCGATCCGTAAAGCGCTGCAAGAATGTATGCAGCAt                                                               <  1:514792/140‑1 (MQ=255)                                                                       tCGCCGAGCAGGGCGCACTGCGCGATGCCAGCGAGTCTGATGTTGAAGCGTCTCTGGATCGCCTGATCCGCTGGAACAATAATCGTAACGGTGAAGATCCGGTGGCGATCCGTAAAGCGCTGCAAGAATGTATGCAGCAt                                                               <  1:954816/140‑1 (MQ=255)                                                                        cGCCGAGCAGGGCGCACTGCGCGATGCCAGCGAGTCTGATGTTGAAGCGTCTCTGGATCGCCTGATCCGCTTGAACAATAATCGTAACGGTGAAGATCCGGTGGCGATCCGTAAAGCGCTGCa                                                                               <  1:802119/123‑1 (MQ=255)                                                                        cGCCGAGCAGGGCGCACTGCGCGATGCCAGCGAGTCTGATGTTGAAGCGTCTCTGGATCGCCTGATCCGCTGGAACAATAATCGTAACGGTGAAGATCCGGTGGCGATCCGTAAAGCGCTGCa                                                                               >  2:802119/1‑123 (MQ=255)                                                                            gAGCAGGGCGCACTGCGCGATGCCAGCGAGTCTGATGTTGAAGCGTCTCTGGATCGCCTGATCCGCTGGAACAATAATCGTAACGGTGAAGATCCGGTGGCGATCCGTAAAGCGCTGCAAGAATGTATGCAGCATAACtt                                                          >  2:1461423/1‑140 (MQ=255)                                                                             aGCAGGGCGCACTGCGCGATGCCAGCGAGTCTGATGTTGAAGCGTCTCTGGATCGCCTGATCCGCTGGAACAATAATCGTAACGGTGAAGATCCGGTGGCGATCCGTAAAGCGCTGCAAGAATGTATGCAGCATAACTtc                                                         >  1:1583769/1‑140 (MQ=255)                                                                                aGGGCGCACTGCGCGATGCCAGCGAGTCTGATGTTGAAGCGTCTCTGGATCGCCTGATCCGCTGGAACAATAATCGTAACGGTGAAGATCCGGTGGCGATCCGTAAAGCGCTGCAAGAATGTATGCAGCATAACTTCTCg                                                      <  1:261484/140‑1 (MQ=255)                                                                                  ggCGCACTGCGCGATGCCAGCGAGTCTGATGTTGAAGCGTCTCTGGATCGCCTGATCCGCTGGAACAATAATCGTAACGGTGAAGATCCGGTGGCGATCCGTAAAGCGCTGCAAGAATGTATGCAGCATAACTTCTCGGt                                                    >  2:1255857/1‑140 (MQ=255)                                                                                   gcgcACTGCGCGATGCCAGCGAGTCTGATGTTGAAGCGTCTCTGGATCGCCTGATCCGCTGGAACAATAATCGTAACGGTGAAGATCCGGTGGCGATCCGTAAAGCGCTGCAAGAATGTATGCAGCATAACTTCTCGGTc                                                   <  1:819918/140‑1 (MQ=255)                                                                                   gcgcACTGCGCGATGCCAGCGAGTCTGATGTTGAAGCGTCTCTGGATCGCCTGATCCGCTGGAACAATAATCGTAACGGTGAAGATCCGGTGGCGATCCGTAAAGCGCTGCAAGAATGTATGCAGCATAACTTCTCGGTc                                                   <  2:1036374/140‑1 (MQ=255)                                                                                       aCTGCGCGATGCCAGCGAGTCTGATGTTGAAGCGTCTCTGGATCGCCTGATCCGCTGGAACAATAATCGTAACGGTGAAGATCCGGGGGCGAGCCGTAAAGCTCTGCAAGAATGTATGCAGCATAACTTCTCGGTCTTcc                                               >  2:1479567/1‑140 (MQ=255)                                                                                          gcgcGATGCCAGCGAGTCTGATGTTGAAGCGTCTCTGGATCGCCTGATCCGCTGGAACAATAATCGTAACGGTGAAGATCCGGTGGCGATCCGTAAAGCGCTGCAAGAATGTATGCAGCATAACTTCTCGGTCTTCCGTg                                            <  2:1057113/140‑1 (MQ=255)                                                                                            gcgATGCCAGCGAGTCTGATGTTGAAGCGTCTCTGGATCGCCTGATCCGCTGGAACAATAATCGTAACGGTGAAGATCCGGTGGCGATCCGTAAAGCGCTGCAAGAATGTATGCAGCATAACTTCTCGGTCTTCCGTGaa                                          >  1:412370/1‑140 (MQ=255)                                                                                            gcgATGCCAGCGAGTCTGATGTTGAAGCGTCTCTGGATCGCCTGATCCGCTGGAACAATAATCGTAACGGTGAAGATCCGGTGGCGATCCGTAAAGCGCTGCAAGAATGTATGCAGCATAACTTCTCGGTCTTCCGTGaa                                          >  1:75310/1‑140 (MQ=255)                                                                                                   cAGCGAGTCTGATGTTGAAGCGTCTCTGGATCGCCTGATCCGCTGGAACAATAATCGTAACGGTGAAGATCCGGTGGCGATCCGTAAAGCGCTGCAAGAATGTATGCAGCATAACTTCTCGGTCTTCCGTGAAGGTGATg                                   <  2:1583769/140‑1 (MQ=255)                                                                                                        aGTCTGATGTTGAAGCGTCTCTGGATCGCCTGATCCGCTGGAACAATAATCGTAACGGTGAAGATCCGGTGGCGATCCGTAAAGCGCTGCAAGAATGTATGCAGCATAACTTCTCGGTCTTCCGTGAAGGTGATGCGATg                              <  2:412370/140‑1 (MQ=255)                                                                                                        aGTCTGATGTTGAAGCGTCTCTGGATCGCCTGATCCGCTGGAACAATAATCGTAACGGTGAAGATCCGGTGGCGATCCGTAAAGCGCTGCAAGAATGTATGCAGCATAACTTCTCGGTCTTCCGTGAAGGTGATGCGATg                              <  2:75310/140‑1 (MQ=255)                                                                                                          tCTGATGTTGAAGCGTCTCTGGATCGCCTGATCCGCTGGAACAATAATCGTAACGGTGAAGATCCGGTGGCGATCCGTAAAGCGCTGCAAGAATGTATGCAGCATAACTTCTCGGTCTTCCGt                                             >  1:951201/1‑123 (MQ=255)                                                                                                          tCTGATGTTGAAGCGTCTCTGGATCGCCTGATCCGCTGGAACAATAATCGTAACGGTGAAGATCCGGTGGCGATCCGTAAAGCGCTGCAAGAATGTATGCAGCATAACTTCTCGGTCTTCCGt                                             <  2:951201/123‑1 (MQ=255)                                                                                                          tCTGATGTTGAAGCGTCTCTGGATCGCCTGATCCGCTGGAACAATAATCGTAACGGTGAAGATCCGGTGGCGATCCGTAAAGCGCTGCAAGAATGTATGCAGCATAACTTCTCGGTCTTCCGTGAAGGTGATGCGATGGc                            >  2:736290/1‑140 (MQ=255)                                                                                                          tCTGATGTTGAAGCGTCTCTGGATCGCCTGATCCGCTGGAACAATAATCGTAACGGTGAAGATCCGGTGGCGATCCGTAAAGCGCTGCAAGAATGTATGCAGCATAACTTCTCGGTCTTCCGTGAAGGTGATGCGATGGc                            >  1:1101501/1‑140 (MQ=255)                                                                                                             gATGTTGAAGCGTCTCTGGATCGCCTGATCCGCTGGAACAATAATCGTAACGGTGAAGATCCGGTGGCGATCCGTAAAGCGCTGCAAGAATGTATGCAGCATAACTTCTCGGTCTTCCGTGAAGGTGATGCGATGGCGaa                         <  2:987592/140‑1 (MQ=255)                                                                                                             gATGTTGAAGCGTCTCTGGATCGCCTGATCCGCTGGAACAATAATCGTAACGGTGAAGATCCGGTGGCGATCCGTAAAGCGCTGCAAGAATGTATGCAGCATAACTTCTCGGTCTTCCGTGAAGGTGATGCGATGGCGaa                         <  2:1188954/140‑1 (MQ=255)                                                                                                             gATGTTGAAGCGTCTCTGGATCGCCTGATCCGCTGGAACAATAATCGTAACGGTGAAGATCCGGTGGCGATCCGTAAAGCGCTGCAAGAATGTATGCAGCATAACTTCTCGGTCTTCCGTGAAGGTGATGCGATGGCGaa                         <  1:1232763/140‑1 (MQ=255)                                                                                                                   gAAGCGTCTCTGGATCGCCTGATCCGCTGGAACAATAATCGTAACGGTGAAGATCCGGTGGCGATCCGTAAAGCGCTGCAAGAATGTATGCAGCATAACTTCTCGGTCTTCCGTGAAGGTGATGCGATGGCGAAAGGGc                    >  1:886692/1‑139 (MQ=255)                                                                                                                   gAAGCGTCTCTGGATCGCCTGATCCGCTGGAACAATAATCGTAACGGTGAAGATCCGGTGGCGATCCGTAAAGCGCTGCAAGAATGTATGCAGCATAACTTCTCGGTCTTCCGTGAAGGTGATGCGATGGCGAAAGGGc                    <  2:886692/139‑1 (MQ=255)                                                                                                                   gAAGCGTCTCTGGATCGCCTGATCCGCTGGAACAATAATCGTAACGGTGAAGATCCGGTGGCGATCCGTAAAGCGCTGCAAGAATGTATGCAGCATAACTTCTCGGTCTTCCGTGAAGGTGATGCGATGGCGAAAGGGCt                   >  1:1020613/1‑140 (MQ=255)                                                                                                                       cGTCTCTGGATCGCCTGATCCGCTGGAACAATAATCGTAACGGTGAAGATCCGGTGGCGATCCGTAAAGCGCTGCAAGAATGTATGCAGCATAACTTCTCGGTCTTCCGTGAAGGTGATGCGATGGCGAAAGGGCTTGAg               <  2:965289/140‑1 (MQ=255)                                                                                                                       cGTCTCTGGATCGCCTGATCCGCTGGAACAATAATCGTAACGGTGAAGATCCGGTGGCGATCCGTAAAGCGCTGCAAGAATGTATGCAGCATAACTTCTCGGTCTTCCGTGAAGGTGATGCGATGGCGAAAGGGCTTGAg               <  1:300886/140‑1 (MQ=255)                                                                                                                         tctcTGGATCGCCTGATCCGCTGGAACAATAATCGTAACGGTGAAGATCCGGTGGCGATCCGTAAAGCGCTGCAAGAATGTATGCAGCATAACTTCTCGGTCTTCCGTGAAGGTGATGCGATGGCGAAAGGGCTTGAGCa             >  2:1276378/1‑140 (MQ=255)                                                                                                                                    ccTGATCCGCTGGAACAATAATCGTAACGGTGAAGATCCGGTGGCGATCCGTAAAGCGCTGCAAGAATGTATGCAGCATAACTTCTCGGTCTTCCGTGAAGGTGATGCGATGGCGAAAGGGCTTGAGCAGTTGAAAGTGa  >  1:106494/1‑140 (MQ=255)                                                                                                                                    ccTGATCCGCTGGAACAATAATCGTAACGGTGAAGATCCGGTGGCGATCCGTAAAGCGCTGCAAGAATGTATGCAGCATAACTTCTCGGTCTTCCGTGAAGGTGATGCGATGGCGAAAGGGCTTGAGCAGTTGAAAGTGa  >  2:1503491/1‑140 (MQ=255)                                                                                                                                    ccTGATCCGCTGGAACAATAATCGTAACGGTGAAGATCCGGTGGCGATCCGTAAAGCGCTGCAAGAATGTATGCAGCATAACTTCTCGGTCTTCCGTGAAGGTGATGCGATGGCGAAAGGGCTTGAGCAGTTGAAAGTGa  >  1:465237/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                       CTGGGCGGCAACTCGCTGCTGGACCTGGTGGTCTTTGGTCGCGCGGCAGGTCTGCATCTGCAAGAGTCTATCGCCGAGCAGGGCGCACTGCGCGATGCCAGCGAGTCTGATGTTGAAGCGTCTCTGGATCGCCTGAACCGCTGGAACAATAATCGTAACGGTGAAGATCCGGTGGCGATCCGTAAAGCGCTGCAAGAATGTATGCAGCATAACTTCTCGGTCTTCCGTGAAGGTGATGCGATGGCGAAAGGGCTTGAGCAGTTGAAAGTGA  >  NC_000913/757104‑757374

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑