Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,166,570	C→T	S162S (TCC→TCT)	ndh →	NADH:quinone oxidoreductase II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,166,570	0	C	T	100.0%	176.0 / NA	52	S162S (TCC→TCT)	ndh	NADH:quinone oxidoreductase II
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (28/24);  total (28/24)

CCTGGTAATGGCGCTGGGTAGCACCTCTAACGATTTCAATACGCCAGGTGTCAAAGAGAACTGCATTTTCCTCGATAACCCGCACCAGGCGCGTCGCTTCCACCAGGAGATGCTGAATTTGTTCCTGAAATACTCCGCCAACCTGGGCGCGAATGGCAAAGTGAACATTGCGATTGTCGGCGGCGGCGCGACGGGTGTAGAACTCTCCGCTGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACG  >  NC_000913/1166435‑1166703                                                                                                                                        |                                                                                                                                      ccTGGTAATGGCGCTGGGTAGCACCTCTAACGATTTCAATACGCCAGGTGTCAAAGAGAACTGCATTTTCCTCGATAACCCGCACCAGGCGCGTCGCTTCCACCAGGAGATGCTGAATTTGTTCCTGAAATACTCTGCCa                                                                                                                                   >  1:1424976/1‑140 (MQ=255)     gTAATGGCGCTGGGTAGCACCTCTAACGATTTCAATACGCCAGGTGTCAAAGAGAACTGCATTTTCCTCGATAACCCGCACCAGGCGCGTCGCTTCCACCAGGAGATGCTGAATTTGTTCCTGAAATACTCTGCCAACCt                                                                                                                               >  2:434841/1‑140 (MQ=255)        aTGGCGCTGGGTAGCACCTCTAACGATTTCAATACGCCAGGTGTCAAAGAGAACTGCATTTTCCTCGATAACCCGCACCAGGCGCGTCGCTTCCACCAGGAGATGCTGAATTTGTTCCTGAAATACTCTGCCAACCTggg                                                                                                                            >  1:1039356/1‑140 (MQ=255)         tGGCGCTGGGTAGCACCTCTAACGATTTCAATACGCCAGGTGTCAAAGAGAACTGCATTTTCCTCGATAACCCGCACCAGGCGCGTCGCTTCCACCAGGAGATGCTGAATTTGTTCCTGAAATACTCTGCCAACCTGGgc                                                                                                                           >  1:1247740/1‑140 (MQ=255)               tGGGTAGCACCTCTAACGATTTCAATACGCCAGGTGTCAAAGAGAACTGCATTTTCCTCGATAACCCGCACCAGGCGCGTCGCTTCCACCAGGAGATGCTGAATTTGTTCCTGAAATACTCTGCCAACCTGGGCGCGAAt                                                                                                                     >  1:407893/1‑140 (MQ=255)                gggTAGCACCTCTAACGATTTCAATACGCCAGGTGTCAAAGAGAACTGCATTTTCCTCGATAACCCGCACCAGGCGCGTCGCTTCCACCAGGAGATGCTGAATTTGTTCCTGAAATACTCTGCCAACCTGGGCGCGAATg                                                                                                                    >  1:414729/1‑140 (MQ=255)                      cACCTCTAACGATTTCAATACGCCAGGTGTCAAAGAGAACTGCATTTTCCTCGATAACCCGCACCAGGCGCGTCGCTTCCACCAGGAGATGCTGAATTTGTTCCTGAAATACTCTGCCAACCTGGGCGCGAATGGCAAAg                                                                                                              >  1:1367654/1‑140 (MQ=255)                        ccTCTAACGATTTCAATACGCCAGGTGTCAAAGAGAACTGCATTTTCCTCGATAACCCGCACCAGGCGCGTCGCTTCCACCAGGAGATGCTGAATTTGTTCCTGAAATACTCTGCCAACCTGGGCGCGAATGGCAAAGTg                                                                                                            <  1:1045285/140‑1 (MQ=255)                          tctAACGATTTCAATACGCCAGGTGTCAAAGAGAACTGCATTTTCCTCGATAACCCGCACCAGGCGCGTCGCTTCCACCAGGAGATGCTGAATTTGTTCCTGAAATACTCTGCCAACCTGGGCGCGAATGGCAAAGTGaa                                                                                                          <  2:1247740/140‑1 (MQ=255)                           ctAACGATTTCAATACGCCAGGTGTCAAAGAGAACTGCATTTTCCTCGATAACCCGCACCAGGCGCGTCGCTTCCACCAGGAGATGCTGAATTTGTTCCTGAAATACTCTGCCAACCTGGGCGCGAATGGCaa                                                                                                                <  2:271761/133‑1 (MQ=255)                           ctAACGATTTCAATACGCCAGGTGTCAAAGAGAACTGCATTTTCCTCGATAACCCGCACCAGGCGCGTCGCTTCCACCAGGAGATGCTGAATTTGTTCCTGAAATACTCTGCCAACCTGGGCGCGAATGGCaa                                                                                                                >  1:271761/1‑133 (MQ=255)                                   ttCAATACGCCAGGTGTCAAAGAGAACTGCATTTTCCTCGATAACCCGCACCAGGCGCGTCGCTTCCACCAGGAGATGCTGAATTTGTTCCTGAAATACTCTGCCAACCTGGGCGCGAATGGc                                                                                                                  >  1:1248432/1‑123 (MQ=255)                                   ttCAATACGCCAGGTGTCAAAGAGAACTGCATTTTCCTCGATAACCCGCACCAGGCGCGTCGCTTCCACCAGGAGATGCTGAATTTGTTCCTGAAATACTCTGCCAACCTGGGCGCGAATGGc                                                                                                                  <  2:1248432/123‑1 (MQ=255)                                      aaTACGCCAGGTGTCAAAGAGAACTGCATTTTCCTCGATAACCCGCACCAGGCGCGTCGCTTCCACCAGGAGATGCTGAATTTGTTCCTGAAATACTCTGCCAACCTGGGCGCGAATGGCAAAGTGAACATTGCGATTGt                                                                                              >  1:885068/1‑140 (MQ=255)                                                   tCAAAGAGAACTGCATTTTCCTCGATAACCCGCACCAGGCGCGTCGCTTCCACCAGGAGATGCTGAATTTGTTCCTGAAATCCTCTGCCAACCTGGGCGCGAATGGCAAAGTGAACATTGCGATTGTCGGCGGCGGcgcg                                                                                 <  2:334324/140‑1 (MQ=255)                                                     aaaGAGAACTGCATTTTCCTCGATAACCCGCACCAGGCGCGTCGCTTCCACCAGGAGATGCTGAATTTGTTCCTGAAATACTCTGCCAACCTGGGCGCGAATGGCAAAGTGAACATTGCGATTGTCGGCGGCGGCGCGAc                                                                               <  2:407893/140‑1 (MQ=255)                                                     aaaGAGAACTGCATTTTCCTCGATAACCCGCACCAGGCGCGTCGCTTCCACCAGGAGATGCTGAATTTGTTCCTGAAATACTCTGCCAACCTGGGCGCGAATGGCAAAGTGAACATTGCGATTGTCGGCGGCGGCGCGAc                                                                               >  2:382687/1‑140 (MQ=255)                                                     aaaGAGAACTGCATTTTCCTCGATAACCCGCACCAGGCGCGTCGCTTCCACCAGGAGATGCTGAATTTGTTCCTGAAATACTCTGCCAACCTGGGCGCGAATGGCAAAGTGAACATTGCGATTGTCGGCGGCGGCGCGAc                                                                               >  1:1498734/1‑140 (MQ=255)                                                     aaaGAGAACTGCATTTTCCTCGATAACCCGCACCAGGCGCGTCGCTTCCACCAGGAGATGCTGAATTTGTTCCTGAAATACTCTGCCAACCTGGGCGCGAATGGCAAAGTGAACATTGCGATTGTCGGCGGCGGCGCGAc                                                                               <  2:1429763/140‑1 (MQ=255)                                                             cTGCATTTTCCTCGATAACCCGCACCAGGCGCGTCGCTTCCACCAGGAGATGCTGAATTTGTTCCTGAAATACTCTGCCAACCTGGGCGCGAATGGCAAAGTGAACATTGCGATTGTCGGCGGCGGCGCGACGGGTGTAg                                                                       >  1:1261621/1‑140 (MQ=255)                                                                 aTTTTCCTCGATAACCCGCACCAGGCGCGTCGCTTCCACCAGGAGATGCTGAATTTGTTCCTGAAATACTCTGCCAACCTGGGCGCGAATGGCAAAGTGAACATTGCGATTGTCGGCGGCGGCGCGACGGGTGTAGAAct                                                                   <  2:1498734/140‑1 (MQ=255)                                                                     tCCTCGATAACCCGCACCAGGCGCGTCGCTTCCACCAGGAGATGCTGAATTTGTTCCTGAAATACTCTGCCAACCTGGGCGCGAATGGCAAAGTGAACATTGCGATTGTCGGCGGCGGCGCGACGGGTGTAGAACTCTcc                                                               >  2:1216443/1‑140 (MQ=255)                                                                      ccTCGATAACCCGCACCAGGCGCGTCGCTTCCACCAGGAGATGCTGAATTTGTTCCTGAAATACTCTGCCAACCTGGGCGCGAATGGCAAAGTGAACATTGCGATTGTCGGCGGCGGCGCGACGGGTGTAGAACTCTCCg                                                              <  2:1261621/140‑1 (MQ=255)                                                                        tCGATAACCCGCACCAGGCGCGTCGCTTCCACCAGGAGATGCTGAATTTGTTCCTGAAATACTCTGCCAACCTGGGCGCGAATGGCAAAGTGAACATTGCGATTGTCGGCGGCGGCGCGACGGGTGTAGAACTCTCCGCt                                                            >  2:88040/1‑140 (MQ=255)                                                                          gATAACCCGCACCAGGCGCGTCGCTTCCACCAGGAGATGCTGAATTTGTTCCTGAAATACTCTGCCAACCTGGGCGCGAATGGCAAAGTGAACATTGCGATTGTCGGCGGCGGCGCGACGGGTGTAGAACTCTCCGCTGa                                                          >  1:1552932/1‑140 (MQ=255)                                                                           aTAACCCGCACCAGGCGCGTCGCTTCCACCAGGAGATGCTGAATTTGTTCCTGAAATACTCTGCCAACCTGGGCGCGAATGGCAAAGTGAACATTGCGATTGTCGGCGGCGGCGCGACGGGTGTAGAACTCTCCGCTGaa                                                         <  1:1216443/140‑1 (MQ=255)                                                                                   cACCAGGCGCGTCGCTTCCACCAGGAGATGCTGAATTTGTTCCTGAAATACTCTGCCAACCTGGGCGCGAATGGCAAAGTGAACATTGCGATTGTCTGCGGCGGCGCGACGGGTGTAGAACTCTCCGCTGAATTGCACaa                                                 <  2:359550/140‑1 (MQ=255)                                                                                    aCCAGGCGCGTCGCTTCCACCAGGAGATGCTGAATTTGTTCCTGAAATACTCTGCCAACCTGGGCGCGAATGGCAAAGTGAACATTGCGATTGTCGGCGGCGGCGCGACGGGTGTAGAACTCTCCGCTGAATTGCACAAc                                                >  1:47496/1‑140 (MQ=255)                                                                                       aGGCGCGTCGCTTCCACCAGGAGATGCTGAATTTGTTCCTGAAATACTCTGCCAACCTGGGCGCGAATGGCAAAGTGAACATTGCGATTGTCGGCGGCGGCGCGACGGGTGTAGAACTCTCCGCTGAATTGCACAAcgcg                                             <  1:88040/140‑1 (MQ=255)                                                                                         gcgcGTCGCTTCCACCAGGAGATGCTGAATTTGTTCCTGAAATACTCTGCCAACCTGGGCGCGAATGGCAAAGTGAACATTGCGATTGTCGGCGGCGGCGCGACGGGTGTAGAACTCTCCGCTGAATTGCACAACGCGGt                                           >  1:1050932/1‑140 (MQ=255)                                                                                              tCGCTTCCACCAGGAGATGCTGAATTTGTTCCTGAAATACTCTGCCAACCTGGGCGCGAATGGCAAAGTGAACATTGCGATTGTCGGCGGCGGCGCGACGGGTGTAGAACTCTCCGCTGAATTGCACAACGCGGTCAAGc                                      >  1:900955/1‑140 (MQ=255)                                                                                              tCGCTTCCACCAGGAGATGCTGAATTTGTTCC‑GAAATACTCTGCCAACCTGGGCGCGAATGGCAAAGTGAACATTGCGATTGTCGGCGGCGGCGCGACGGGTGTAGAACTCTCCGCTGAATTGCACAACGCGGTCAAGCa                                     >  1:1577673/1‑140 (MQ=255)                                                                                                gCTTCCACCAGGAGATGCTGAATTTGTTCCTGAAATACTCTGCCAACCTGGGCGCGAATGGCAAAGTGAACATTGCGATTGTCGGCGGCGGCGCGACGGGTGTAGAACTCTCCGCTGAATTGCACAACGCGGTCAAGCaa                                    <  2:1039356/140‑1 (MQ=255)                                                                                                gCTTCCACCAGGAGATGCTGAATTTGTTCCTGAAATACTCTGCCAACCTGGGCGCGAATGGCAAAGTGAACATTGCGATTGTCGGCGGCGGCGCGACGGGTGTAGAACTCTCCGCTGAATTGCACAACGCGGTCAAGCaa                                    <  2:1353945/140‑1 (MQ=255)                                                                                                     caccaGGAGATGCTGAATTTGTTCCTGAAATACTCTGCCAACCTGGGCGCGAATGGCAAAGTGAACATTGCGATTGTCGGCGGCGGCGCGACGGGTGTAGAACTCTCCGCTGAATTGCACAACGCGGTCAAGCAACTGca                               <  2:900955/140‑1 (MQ=255)                                                                                                          ggAGATGCTGAATTTGTTCCTGAAATACTCTGCCAACCTGGGCGCGAATGGCAAAGTGAACATTGCGATTGTCGGCGGCGGCGCGACGGGTGTAGAACTCTCCGCTGAATTGCACAACGCGGTCAAGCAACTGCACAGCt                          >  2:885802/1‑140 (MQ=255)                                                                                                          ggAGATGCTGAATTTGTTCCTGAAATACTCTGCCAACCTGGGCGCGAATGGCAAAGTGAACATTGCGATTGTCGGCGGCGGCGCGACGGGTGTAGAACTCTCCGCTGAATTGCACAACGCGGTCAAGCAACTGCACAGCt                          <  1:382687/140‑1 (MQ=255)                                                                                                           gagaTGCTGAATTTGTTCCTGAAATACTCTGCCAACCTGGGCGCGAATGGCAAAGTGAACATTGCGATTGTCGGCGGCGGCGCGACGGGTGTAGAACTCTCCGCTGAATTGCACAACGCGGTCAAGCAACTGCACAGCTa                         >  2:796485/1‑140 (MQ=255)                                                                                                            agaTGCTGAATTTGTTCCTGAAATACTCTGCCAACCTGGGCGCGAATGGCAAAGTGAACATTGCGATTGTCGGCGGCGGCGCGACGGGTGTAGAACTCTCCGCTGAATTGCACAACGCGGTCAAGCAACTGCACAGCTAc                        <  2:152999/140‑1 (MQ=255)                                                                                                              aTGCTGAATTTGTTCCTGAAATACTCTGCCAACCTGGGCGCGAATGGCAAAGTGAACATTGCGATTGTCGGCGGCGGCGCGACGGGTGTAGAACTCTCCGCTGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACgg                      >  2:117660/1‑140 (MQ=255)                                                                                                              aTGCTGAATTTGTTCCTGAAATACTCTGCCAACCTGGGCGCGAATGGCAAAGTGAACATTGCGATTGTCGGCGGCGGCGCGACGGGTGTAGAACTCTCCGCTGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACgg                      <  2:155861/140‑1 (MQ=255)                                                                                                              aTGCTGAATTTGTTCCTGAAATACTCTGCCAACCTGGGCGCGAATGGCAAAGTGAACATTGCGATTGTCGGCGGCGGCGCGACGGGTGTAGAACTCTCCGCTGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACgg                      <  1:244296/140‑1 (MQ=255)                                                                                                                gCTGAATTTGTTCCTGAAATACTCTGCCAACCTGGGCGCGAATGGCAAAGTGAACATTGCGATTGTCGGTGGCGGCGCGACGGGTGTAGAACTCTCCGCTGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGtt                    >  1:44023/1‑140 (MQ=255)                                                                                                                gCTGAATTTGTTCCTGAAATACTCTGCCAACCTGGGCGCGAATGGCAAAGTGAACATTGCGATTGTCGGCGGCGGCGCGACGGGTGTAGAACTCTCCGCTGAATTGCACAACGCGGTCAAGCaa                                    >  1:1044257/1‑124 (MQ=255)                                                                                                                gCTGAATTTGTTCCTGAAATACTCTGCCAACCTGGGCGCGAATGGCAAAGTGAACATTGCGATTGTCGGCGGCGGCGCGACGGGTGTAGAACTCTCCGCTGAATTGCACAACGCGGTCAAGCaa                                    <  2:1044257/124‑1 (MQ=255)                                                                                                                gCTGAATTTGTTCCTGAAATACTCTGCCAACCTGGGCGCGAATGGCAAAGTGAACATTGCGATTGTAGGCGGCGGCGCGACGGGTGTAGAACTCTCCGCTGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGtt                    >  1:1373811/1‑140 (MQ=255)                                                                                                                 cTGAATTTGTTCCTGAAATACTCTGCCAACCTGGGCGCGAATGGCAAAGTGAACATTGCGATTGTCGGCGGCGGCGCGACGGGTGTAGAACTCTCCGCTGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTa                   >  1:1445486/1‑140 (MQ=255)                                                                                                                   gAATTTGTTCCTGAAATACTCTGCCAACCTGGGCGCGAATGGCAAAGTGAACATTGCGATTGTCGGCGGCGGCGCGACGGGTGTAGAACTCTCCGCTGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACa                 <  2:414729/140‑1 (MQ=255)                                                                                                                   gAATTTGTTCCTGAAATACTCTGCCAACCTGGGCGCGAATGGCAAAGTGAACATTGCGATTGTCGGCGGCGGCGCGACGGGTGTAGAACTCTCCGCTGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACa                 <  1:434841/140‑1 (MQ=255)                                                                                                                          ttCCTGAAATACTCTGCCAACCTGGGCGCGAATGGCAAAGTGAACATTGCGATTGTCGGCGGCGGCGCGACGGGTGTAGAACTCTCCGCTGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCt          >  1:1465404/1‑140 (MQ=255)                                                                                                                             cTGAAATACTCTGCCAACCTGGGCGCGAATGGCAAAGTGAACATTGCGATTGTCGGCGGCGGCGCGACGGGTGTAGAACTCTCCGCTGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGAc       <  2:1367654/140‑1 (MQ=255)                                                                                                                                  aTACTCTGCCAACCTGGGCGCGAATGGCAAAGTGAACATTGCGATTGTCGGCGGCGGCGCGACGGGTGTAGAACTCTCCGCTGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACg  <  1:117660/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                      CCTGGTAATGGCGCTGGGTAGCACCTCTAACGATTTCAATACGCCAGGTGTCAAAGAGAACTGCATTTTCCTCGATAACCCGCACCAGGCGCGTCGCTTCCACCAGGAGATGCTGAATTTGTTCCTGAAATACTCCGCCAACCTGGGCGCGAATGGCAAAGTGAACATTGCGATTGTCGGCGGCGGCGCGACGGGTGTAGAACTCTCCGCTGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACG  >  NC_000913/1166435‑1166703

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑