Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,316,169	A→C	intergenic (‑134/+247)	yciG ← / ← trpA	protein YciG/tryptophan synthase subunit alpha

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,316,169	0	A	C	100.0%	267.7 / NA	69	intergenic (‑134/+247)	yciG/trpA	protein YciG/tryptophan synthase subunit alpha
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (33/36);  total (33/36)

CCATGTTATTTCTCCCGTTGCGTTGCATTGTTTCATTAATATGAGTGTTGTGTGTCGACACTCATTAAAATTAGTCGCTAATGAGAATTAGTCAAATTAAGCGCAACGAGAAGATAGAGGGAAAATATATTTTGAGGAACATTCTGGATATATTAACAATTACCTGAGGAATAAGTGACTTAGAGAAAATTATTGATTTTACTGGTGTTATGTTGCGGGATTAATTTGTTTGATTATATTACTGTTGGGCGGAAAAATGACGTAAGTTGACGTT  >  NC_000913/1316032‑1316305                                                                                                                                          |                                                                                                                                         ccATGTTATTTCTCCCGTTGCGTTGCATTGTTTCATTAATATGAGTGTTGTGTGTCGACACTCATTAAAATTAGTCGCTAATGAGAATTAGTCAAATTAAGCGCAACGAGAAGATAGAGGGAAAATATATTTTGAGGCAc                                                                                                                                        >  1:77232/1‑140 (MQ=255)  cATGTTATTTCTCCCGTTGCGTTGCATTGTTTCATTAATATGAGTGTTGTGTGTCGACACTCATTAAAATTAGTCGCTAATGAGAATTAGTCAAATTAAGCGCAACGAGAAGATAGAGGGAAAATATATTTTGAGGcaca                                                                                                                                       >  2:211766/1‑140 (MQ=255)   aTGTTATTTCTCCCGTTGCGTTGCATTGTTTCATTAATATGAGTGTTGTGTGTCGACACTCATTAAAATTAGTCGCTAATGAGAATTAGTCAAATTAAGCGCAACGAGAAGATAGAGGGAAAATATATTTTGAGGCACAt                                                                                                                                      <  1:490475/140‑1 (MQ=255)    tGTTATTTCTCCCGTTGCGTTGCATTGTTTCATTAATATGAGTGTTGTGTGTCGACACTCATTAAAATTAGTCGCTAATGAGAATTAGTCAAATTAAGCGCAACGAGAAGATAGAGGGAAAATATATTTTGAGGCACAtt                                                                                                                                     <  2:464568/140‑1 (MQ=255)    tGTTATTTCTCCCGTTGCGTTGCATTGTTTCATTAATATGAGTGTTGTGTGTCGACACTCATTAAAATTAGTCGCTAATGAGAATTAGTCAAATTAAGCGCAACGAGAAGATAGAGGGAAAATATATTTTGAGGCACAtt                                                                                                                                     <  1:159630/140‑1 (MQ=255)      ttATTTCTCCCGTTGCGTTGCATTGTTTCATTAATATGAGTGTTGTGTGTCGACACTCATTAAAATTAGTCGCTAATGAGAATTAGTCAAATTAAGCGCAACGAGAAGATAGAGGGAAAATATATTTTGAGGCACATTCt                                                                                                                                   >  1:83632/1‑140 (MQ=255)       tATTTCTCCCGTTGCGTTGCATTGTTTCATTAATATGAGTGTTGTGTGTCGACACTCATTAAAATTAGTCGCTAATGAGAATTAGTCAAATTAAGCGCAACGAGAAGATAGAGGGAAAATATATTTTGAGGCACATTCTg                                                                                                                                  <  2:83632/140‑1 (MQ=255)       tATTTCTCCCGTTGCGTTGCATTGTTTCATTAATATGAGTGTTGTGTGTCGACACTCATTAAAATTAGTCGCTAATGAGAATTAGTCAAATTAAGCGCAACGAGAAGATAGAGGGAAAATATATTTTGAGGCACATTCTg                                                                                                                                  <  2:1212494/140‑1 (MQ=255)         tttCTCCCGTTGCGTTGCATTGTTTCATTAATATGAGTGTTGTGTGTCGACACTCATTAAAATTAGTCGCTAATGAGAATTAGTCAAATTAAGCGCAACGAGAAGATAGAGGGAAAATATATTTTGAGGCACATTCTGGa                                                                                                                                <  1:211766/140‑1 (MQ=255)          ttCTCCCGTTGCGTTGCATTGTTTCATTAATATGAGTGTTGTGTGTCGACACTCATTAAAATTAGTCGCTAATGAGAATTAGTCAAATTAAGCGCAACGAGAAGATAGAGGGAAAATATATTTTGAGGCACATTCTGGat                                                                                                                               <  1:403246/140‑1 (MQ=255)              cccGTTGCGTTGCATTGTTTCATTAATATGAGTGTTGTGTGTCGACACTCATTAAAATTAGTCGCTAATGAGAATTAGTCAAATTAAGCGCAACGAGAAGATAGAGGGAAAATATATTTTGAGGCACATTCTGGatatat                                                                                                                           >  1:1075871/1‑140 (MQ=255)              cccGTTGCGTTGCATTGTTTCATTAATATGAGTGTTGTGTGTCGACACTCATTAAAATTAGTCGCTAATGAGAATTAGTCAAATTAAGCGCAACGAGAAGATAGAGGGAAAATATATTTTGAGGCACATTCTGGatatat                                                                                                                           <  1:1363445/140‑1 (MQ=255)               ccGTTGCGTTGCATTGTTTCATTAATATGAGTGTTGTGTGTCGACACTCATTAAAATTAGTCGCTAATGAGAATTAGTCAAATTAAGCGCAACGAGAAGATAGAGGGAAAATATATTTTGAGGCACATTCTGGATATAtt                                                                                                                          >  2:1216532/1‑140 (MQ=255)                  ttgcgttgcATTGTTTCATTAATATGAGTGTTGTGTGTCGACACTCATTAAAATTAGTCGCTAATGAGAATTAGTCAAATTAAGCGCAACGAGAAGATAGAGGGAAAATATATTTTGAGGCACATTCTGGATATATTAAc                                                                                                                       >  1:440662/1‑140 (MQ=255)                   tgcgttgcATTGTTTCATTAATATGAGTGTTGTGTGTCGACACTCATTAAAATTAGTCGCTAATGAGAATTAGTCAAATTAAGCGCAACGAGAAGATAGAGGGAAAATATATTTTGAGGCACAtt                                                                                                                                     >  1:414943/1‑125 (MQ=255)                   tgcgttgcATTGTTTCATTAATATGAGTGTTGTGTGTCGACACTCATTAAAATTAGTCGCTAATGAGAATTAGTCAAATTAAGCGCAACGAGAAGATAGAGGGAAAATATATTTTGAGGCACAtt                                                                                                                                     <  2:414943/125‑1 (MQ=255)                   tgcgttgcATTGTTTCATTAATATGAGTGTTGTGTGTCGACACTCATTAAAATTAGTCGCTAATGAGAATTAGTCAAATTAAGCGCAACGAGAAGATAGAGGGAAAATATATTTTGAGGCACATTCTGGATATATTAACa                                                                                                                      >  2:692140/1‑140 (MQ=255)                         gcATTGTTTCATTAATATGAGTGTTGTGTGTCGACACTCATTAAAATTAGTCGCTAATGAGAATTAGTCAAATTAAGCGCAACGAGAAGATAGAGGGAAAATATATTTTGAGGCACATTCTGGATATATTAACAATTAcc                                                                                                                <  1:574595/140‑1 (MQ=255)                             tGTTTCATTAATATGAGTGTTGTGTGTCGACACTCATTAAAATTAGTCGCTAATGAGAATTAGTCAAATTAAGCGCAACGAGAAGATAGAGGGAAAATATATTTTGAGGCACATTCTGGATATATTAACAATTACCTGAg                                                                                                            >  1:1426050/1‑140 (MQ=255)                                    ttAATATGAGTGTTGTGTGTCGACACTCATTAAAATTAGTCGCTAATGAGAATTAGTCAAATTAAGCGCAACGAGAAGATAGAGGGAAAATATATTTTGAGGCACATTCTGGATATATTAACAATTACCTg                                                                                                              >  2:132271/1‑131 (MQ=255)                                    ttAATATGAGTGTTGTGTGTCGACACTCATTAAAATTAGTCGCTAATGAGAATTAGTCAAATTAAGCGCAACGAGAAGATAGAGGCAAAATATATTTTGAGGCACATTCTGGATATATTAACAATTACCTg                                                                                                              <  1:132271/131‑1 (MQ=255)                                         atGAGTGTTGTGTGTCGACACTCATTAAAATTAGTCGCTAATGAGAATTAGTCAAATTAAGCGCAACGAGAAGATAGAGGGAAAATATATTTTGAGGCACATTCTGGATATATTAACAATTACCTGAGGAATAAGTGACt                                                                                                >  1:256530/1‑140 (MQ=255)                                            aGTGTTGTGTGTCGACACTCATTAAAATTAGTCGCTAATGAGAATTAGTCAAATTAAGCGCAACGAGAAGATAGAGGGAAAATATATTTTGAGGCACATTCTGGATATATTAACAATTACCTGAGGAATAAGTGACTTag                                                                                             >  2:432496/1‑140 (MQ=255)                                                ttgtGTGTCGACACTCATTAAAATTAGTCGCTAATGAGAATTAGTCAAATTAAGCGCAACGAGAAGATAGAGGGAAAATATATTTTGAGGCACATTCTGGATATATTAACAATTACCTGAGGAATAAGTGACTTAGAGaa                                                                                         >  1:830508/1‑140 (MQ=255)                                                ttgtGTGTCGACACTCATTAAAATTAGTCGCTAATGAGAATTAGTCAAATTAAGCGCAACGAGAAGATAGAGGGAAAATATATTTTGAGGCACATTCTGGATATATTAACAATTACCTGAGGAATAAGTGACTTAGAGaa                                                                                         >  1:66845/1‑140 (MQ=255)                                                  gtgtgtCGACACTCATTAAAATTAGTCGCTAATGAGAATTAGTCAAATTAAGCGCAACGAGAAGATAGAGGGAAAATATATTTTGAGGCACATTCTGGATATATTAACAATTACCTGAGGAATAAGTGACTTAGAGaaaa                                                                                       >  2:785860/1‑140 (MQ=255)                                                  gtgtgtCGACACTCATTAAAATTAGTCGCTAATGAGAATTAGTCAAATTAAGCGCAACGAGAAGATAGAGGGAAAATATATTTTGAGGCACATTCTGGATATATTAACAATTACCTGAGGAATAAGTGACTTAGAGaaaa                                                                                       >  2:881709/1‑140 (MQ=255)                                                  gtgtgtCGACACTCATTAAAATTAGTCGCTAATGAGAATTAGTCAAATTAAGCGCAACGAGAAGATAGAGGGAAAATATATTTTGAGGCACATTCTGGATATATTAACAATTACCTGAGGAATAAGTGACTTAGAGaaaa                                                                                       >  2:24154/1‑140 (MQ=255)                                                   tgtgtCGACACTCATTAAAATTAGTCGCTAATGAGAATTAGTCAAATTAAGCGCAACGAGAAGATAGAGGGAAAATATATTTTGAGGCACATTCTGGATATATTAACAATTACCTGAGGAATAAGTGACTTAGAGAAAat                                                                                      >  2:990156/1‑140 (MQ=255)                                                   tgtgtCGACACTCATTAAAATTAGTCGCTAATGAGAATTAGTCAAATTAAGCGCAACGAGAAGATAGAGGGAAAATATATTTTGAGGCACATTCTGGATATATTAACAATTACCTGAGGAATAAGTGACTTAGAGAAAat                                                                                      >  1:1106539/1‑140 (MQ=255)                                                     tgtCGACACTCATTAAAATTAGTCGCTAATGAGAATTAGTCAAATTAAGCGCAACGAGAAGATAGAGGGAAAATATATTTTGAGGCACATTCTGGATATATTAACAATTACCTGAGGAATAAGTGACTTAGAGAAAatta                                                                                    >  1:535696/1‑140 (MQ=255)                                                     tgtCGACACTCATTAAAATTAGTCGCTAATGAGAATTAGTCAAATTAAGCGCAACGAGAAGATAGAGGGAAAATATATTTTGAGGCACATTCTGGATATATTAACAATTACCTGAGGAATAAGTGACTTAGAGAAAatta                                                                                    >  2:1519400/1‑140 (MQ=255)                                                       tCGACACTCATTAAAATTAGTCGCTAATGAGAATTAGTCAAATTAAGCGCAACGAGAAGATAGAGGGAAAATATATTTTGAGGCACATTCTGGATATATTAACAATTACCTGAGGAATAAGTGACTTAGAGAAAattatt                                                                                  <  2:535696/140‑1 (MQ=255)                                                        cGACACTCATTAAAATTAGTCGCTAATGAGAATTAGTCAAATTAAGCGCAACGAGAAGATAGAGGGAAAATATATTTTGAGGCACATTCTGGATATATTAACAATTACCTGAGGAATAAGTGACTTAGAGAAAATTATTg                                                                                 >  1:1296495/1‑140 (MQ=255)                                                          acacTCATTAAAATTAGTCGCTAATGAGAATTAGTCAAATTAAGCGCAACGAGAAGATAGAGGGAAAATATATTTTGAGGCACATTCTGGATATATTAACAATTACCTGAGGAATAAGTGACTTAGAGAAAATTATTGAt                                                                               >  2:735600/1‑140 (MQ=255)                                                              tCATTAAAATTAGTCGCTAATGAGAATTAGTCAAATTAAGCGCAACGAGAAGATAGAGGGAAAATATATTTTGAGGCACATTCTGGATATATTAACAATTACCTGAGGAATAAGTGACTTAGAGAAAATTATTGATTTTa                                                                           <  2:810838/140‑1 (MQ=255)                                                               cATTAAAATTAGTCGCTAATGAGAATTAGTCAAATTAAGCGCAACGAGAAGATAGAGGGAAAATATATTTTGAGGCACATTCTGGATATATTAACAATTACCTGAGGAATAAGTGACTTAGAGAAAATTATTGATTTTAc                                                                          <  1:24154/140‑1 (MQ=255)                                                               cATTAAAATTAGTCGCTAATGAGAATTAGTCAAATTAAGCGCAACGAGAAGATAGAGGGAAAATATATTTTGAGGCACATTCTGGATATATTAACAATTACCTGAGGAATAAGTGACTTAGAGAAAATTATTGATTTTAc                                                                          <  1:1519400/140‑1 (MQ=255)                                                               cATTAAAATTAGTCGCTAATGAGAATTAGTCAAATTAAGCGCAACGAGAAGATAGAGGGAAAATATATTTTGAGGCACATTCTGGATATATTAACAATTACCTGAGGAATAAGTGACTTAGAGAAAATTATTGATTTTAc                                                                          <  2:20502/140‑1 (MQ=255)                                                               cATTAAAATTAGTCGCTAATGAGAATTAGTCAAATTAAGCGCAACGAGAAGATAGAGGGAAAATATATTTTGAGGCACATTCTGGATATATTAACAATTACCTGAGGAATAAGTGACTTAGAGAAAATTATTGATTTTAc                                                                          <  1:1254984/140‑1 (MQ=255)                                                               cATTAAAATTAGTCGCTAATGAGAATTAGTCAAATTAAGCGCAACGAGAAGATAGAGGGAAAATATATTTTGAGGCACATTCTGGATATATTAACAATTACCTGAGGAATAAGTGACTTAGAGAAAATTATTGATTTTAc                                                                          <  1:735600/140‑1 (MQ=255)                                                               cATTAAAATTAGTCGCTAATGAGAATTAGTCAAATTAAGCGCAACGAGAAGATAGAGGGAAAATATATTTTGAGGCACATTCTGGATATATTAACAATTACCTGAGGAATAAGTGACTTAGAGAAAATTATTGATTTTAc                                                                          <  2:1044973/140‑1 (MQ=255)                                                               cATTAAAATTAGTCGCTAATGAGAATTAGTCAAATTAAGCGCAACGAGAAGATAGAGGGAAAATATATTTTGAGGCACATTCTGGATATATTAACAATTACCTGAGGAATAAGTGACTTAGAGAAAATTATTGATTTTAc                                                                          <  1:1216532/140‑1 (MQ=255)                                                               cATTAAAATTAGTCGCTAATGAGAATTAGTCAAATTAAGCGCAACGAGAAGATAGAGGGAAAATATATTTTGAGGCACATTCTGGATATATTAACAATTACCTGAGGAATAAGTGACTTAGAGAAAATTATTGATTTTAc                                                                          <  2:1325358/140‑1 (MQ=255)                                                               cATTAAAATTAGTCGCTAATGAGAATTAGTCAAATTAAGCGCAACGAGAAGATAGAGGGAAAATATATTTTGAGGCACATTCTGGATATATTAACAATTACCTGAGGAATAAGTGACTTAGAGAAAATTATTGATTTTAc                                                                          >  2:130213/1‑140 (MQ=255)                                                               cATTAAAATTAGTCGCTAATGAGAATTAGTCAAATTAAGCGCAACGAGAAGATAGAGGGAAAATATATTTTGAGGCACATTCTGGATATATTAACAATTACCTGAGGAATAAGTGACTTAGAGAAAATTATTGATTTTAc                                                                          <  2:1296495/140‑1 (MQ=255)                                                                 ttAAAATTAGTCGCTAATGAGAATTAGTCAAATTAAGCGCAACGAGAAGATAGAGGGAAAATATATTTTGAGGCACATTCTGGATATATTAACAATTACCTGAGGAATAAGTGACTTAGAGAAAATTATTGATTTTACTg                                                                        >  2:1094074/1‑140 (MQ=255)                                                                 ttAAAATTAGTCGCTAATGAGAATTAGTCAAATTAAGCGCAACGAGAAGATAGAGGGAAAATATATTTTGAGGCACATTCTGGATATATTAACAATTACCTGAGGAATAAGTGACTTAGAGAAAATTATTGATTTTACTg                                                                        >  1:1590285/1‑140 (MQ=255)                                                                 ttAAAATTAGTCGCTAATGAGAATTAGTCAAATTAAGCGCAACGAGAAGATAGAGGGAAAATATATTTTGAGGCACATTCTGGATATATTAACAATTACCTGAGGAATAAGTGACTTAGAGAAAATTATTGATTTTACTg                                                                        <  2:830508/140‑1 (MQ=255)                                                                        tAGTCGCTAATGAGAATTAGTCAAATTAAGCGCAACGAGAAGATAGAGGGAAAATATATTTTGAGGCACATTCTGGATATATTAACAATTACCTGAGGAATAAGTGACTTAGAGAAAATTATTGATTTTACTGGTGTTAt                                                                 >  1:1296256/1‑140 (MQ=255)                                                                            cGCTAATGAGAATTAGTCAAATTAAGCGCAACGAGAAGATAGAGGGAAAATATATTTTGAGGCACATTCTTGATATATTAACAATTCCCTGAGGAATAAGTGACTTAGAGAAAATTATTGATTTTACTGGTTTTATGTTg                                                             <  2:77232/140‑1 (MQ=255)                                                                            cGCTAATGAGAATTAGTCAAATTAAGCGCAACGAGAAGATAGAGGGAAAATATATTTTGAGGCACATTCTGGATATATTAACAATTACCTGAGGAATAAGTGACTTAGAGAAAATTATTGATTTTACTGGTGTTATGTTg                                                             <  1:1094074/140‑1 (MQ=255)                                                                              cTAATGAGAATTAGTCAAATTAAGCGCAACGAGAAGATAGAGGGAAAATATATTTTGAGGCACATTCTGGATATATTAACAATTACCTGAGGAATAAGTGACTTAGAGAAAATTATTGATTTTACTGGTGTTATGTTGCg                                                           <  2:14427/140‑1 (MQ=255)                                                                                     gaATTAGTCAAATTAAGCGCAACGAGAAGATAGAGGGAAAATATATTTTGAGGCACATTCTGGATATATTAACAATTACCTGAGGAATAAGTGACTTAGAGAAAATTATTGATTTTACTGGTGTTATGTTGCGGGATTaa                                                    <  2:440662/140‑1 (MQ=255)                                                                                          aGTCAAATTAAGCGCAACGAGAAGATAGAGGGAAAATATATTTTGAGGCACATTCTGGATATATTAACAATTACCTGAGGAATAAGTGACTTAGAGAAAATTATTGATTTTACTGGTGTTATGTTGCGGGATTAAtttgt                                               <  2:256530/140‑1 (MQ=255)                                                                                           gTCAAATTAAGCGCAACGAGAAGATAGAGGGAAAATATATTTTGAGGCACATTCTGGATATATTAACAATTACCTGAGGAATAAGTGACTTAGAGAAAATTATTGATTTTACTGGTGTTATGTTGCGGGATTAAtttgtt                                              >  1:1421318/1‑140 (MQ=255)                                                                                            tCAAATTAAGCGCAACGAGAAGATAGAGGGAAAATATATTTTGAGGCACATTCTGGATATATTAACAATTACCTGAGGAATAAGTGACTTAGAGAAAATTATTGATTTTACTGGTGTTATGTTGCGGGATTAAtttgttt                                             <  2:1296256/140‑1 (MQ=255)                                                                                            tCAAATTAAGCGCAACGAGAAGATAGAGGGAAAATATATTTTGAGGCACATTCTGGATATATTAACAATTACCTGAGGAATAAGTGACTTAGAGAAAATTATTGATTTTACTGGTGTTATGTTGCGGGATTAAtttgttt                                             <  2:1426050/140‑1 (MQ=255)                                                                                              aaaTTAAGCGCAACGAGAAGATAGAGGGAAAATATATTTTGAGGCACATTCTGGATATATTAACAATTACCTGAGGAATAAGTGACTTAGAGAAAATTATTGATTTTACTGGTGTTATGTTGCGGGATTAATTTGTTTGa                                           <  2:531399/140‑1 (MQ=255)                                                                                              aaaTTAAGCGCAACGAGAAGATAGAGGGAAAATATATTTTGAGGCACATTCTGGATATATTAACAATTACCTGAGGAATAAGTGACTTAGAGAAAATTATTGATTTTACTGGTGTTATGTTGCGGGATTAATTTGTTTGa                                           <  2:66845/140‑1 (MQ=255)                                                                                              aaaTTAAGCGCAACGAGAAGATAGAGGGAAAATATATTTTGAGGCACATTCTGGATATATTAACAATTACCTGAGGAATAAGTGACTTAGAGAAAATTATTGATTTTACTGGTGTTATGTTGCGGGATTAATTTGTTTGa                                           <  2:1421318/140‑1 (MQ=255)                                                                                                aTTAAGCGCAACGAGAAGATAGAGGGAAAATATATTTTGAGGCAAATTCTGGATATATTAACAATTACCTGAGGAATAAGTGACTTAGAGAAAATTATTGATTTTACTGGTGTTATGTTGCGGGATTAATTTGTTTGAtt                                         >  2:378551/1‑140 (MQ=255)                                                                                                    aGCGCAACGAGAAGATAGAGGGAAAATATATTTTGAGGCACATTCTGGATATATTAACAATTACCTGAGGAATAAGTGACTTAGAGAAAATTATTGATTTTACTGGTGTTATGTTGCGGGATTAATTTGTTTGATTatat                                     <  2:1106539/140‑1 (MQ=255)                                                                                                      cgcAACGAGAAGATAGAGGGAAAATATATTTTGAGGCACATTCTGGATATATTAACAATTACCTGAGGAATAAGTGACTTAGAGAAAATTATTGATTTTACTGGTGTTATGTTGCGGGATTAATTTGTTTGATTATATTa                                   >  1:195453/1‑140 (MQ=255)                                                                                                              gaagaTAGAGGGAAAATATATTTTGAGGCACATTCTGGATATATTAACAATTACCTGAGGAATAAGTGACTTAGAGAAAATTATTGATTTTACTGGTGTTATGTTGCGGGATTAATTTGTTTGATTATATTACTGTTggg                           <  2:788043/140‑1 (MQ=255)                                                                                                              gaagaTAGAGGGAAAATATATTTTGAGGCAAATTCTGGATATATTAACAATTACCTGAGGAATAAGTGACTTAGAGAAAATTATTGATTTTACTGGTGTTATGTTGCGGGATTAATTTGTTTGATTATATTACTGTTggg                           <  1:378551/140‑1 (MQ=255)                                                                                                                 gaTAGAGGGAAAATATATTTTGAGGCACATTCTGGATATATTAACAATTACCTGAGGAATAAGTGACTTAGAGAAAATTATTGATTTTACTGGTGTTATGTTGCGGGATTAATTTGTTTGATTATATTACTGTTGGGCgg                        <  2:195453/140‑1 (MQ=255)                                                                                                                      agGGAAAATATATTTTGAGGCACATTCTGGATATATTAACAATTACCTGAGGAATAAGTGACTTAGAGAAAATTATTGATTTTACTGGTGTTATGTTGCGGGATTAATTTGTTTGATTATATTACTGTTGGGCGGaaaaa                   >  1:1246705/1‑140 (MQ=255)                                                                                                                           aaaTATATTTTGAGGCACATTCTGGATATATTAACAATTACCTGAGGAATAAGTGACTTAGAGAAAATTATTGATTTTACTGGTGTTATGTTGCGGGATTAATTTGTTTGATTATATTACTGTTGGGCGGAAAAATGACg              >  2:1460401/1‑140 (MQ=255)                                                                                                                            aaTATATTTTGAGGCACATTCTGGATATATTAACAATTACCTGAGGAATAAGTGACTTAGAGAAAATTATTGATTTTACTGGTGTTATGTTGCGGGATTAATTTGTTTGATTATATTACTGTTGGGCGGAAAAATGACGt             >  2:676342/1‑140 (MQ=255)                                                                                                                                   tttGAGGCACATTCTGGATATATTAACAATTACCTGAGGAATAAGTGACTTAGAGAAAATTATTGATTTTACTGGTGTTATGTTGCGGGATTAATTTGTTTGATTATATTACTGTTGGGCGGAAAAATGACGTAAGTTGa      >  1:1164388/1‑140 (MQ=255)                                                                                                                                       aGGCACATTCTGGATATATTAACAATTACCTGAGGAATAAGTGACTTAGAGAAAATTATTGATTTTACTGGTGTTATGTTGCGGGATTAATTTGTTTGATTATATTACTGTTGGGCGGAAAAATGACGTAAGTTGACGtt  >  2:698836/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                         CCATGTTATTTCTCCCGTTGCGTTGCATTGTTTCATTAATATGAGTGTTGTGTGTCGACACTCATTAAAATTAGTCGCTAATGAGAATTAGTCAAATTAAGCGCAACGAGAAGATAGAGGGAAAATATATTTTGAGGAACATTCTGGATATATTAACAATTACCTGAGGAATAAGTGACTTAGAGAAAATTATTGATTTTACTGGTGTTATGTTGCGGGATTAATTTGTTTGATTATATTACTGTTGGGCGGAAAAATGACGTAAGTTGACGTT  >  NC_000913/1316032‑1316305

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑