Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,740,909	0	T	G	25.3%	156.7 / 23.4	84	V402G (GTG→GGG)	avtA	valine‑‑pyruvate aminotransferase
Reads supporting (aligned to +/- strand):  ref base T (19/43);  new base G (21/0);  total (41/43)
Fisher's exact test for biased strand distribution p-value = 5.35e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

TCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGA‑TTCTGGCGGAAGAGATAGAAAGAGCCTGGGCTGAAAGTCACTAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATGATTTCTGCAATATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTA  >  NC_000913/3740772‑3741048                                                                                                                                          |                                                                                                                                             tCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGa                                                                                                                                            >  1:1155955/1‑140 (MQ=255) tCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGGGa                                                                                                                                            >  1:1549458/1‑140 (MQ=255)   aGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGGGAAg                                                                                                                                          >  2:1301883/1‑140 (MQ=255)      gcCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGCCTGGATAAACCGTGGCCGCATACGCATCAGTGCATGCGCATGCACTACGTACCAGAGCCGGAGTAAATTGAGGCGGGGGAGAAGA‑tt                                                                                                                                      >  1:1618947/1‑140 (MQ=255)       ccTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGA‑TTc                                                                                                                                     <  2:689982/140‑1 (MQ=255)       ccTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGA‑TTc                                                                                                                                     <  2:1195989/140‑1 (MQ=255)         tGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGA‑TTCTg                                                                                                                                   >  2:1032681/1‑140 (MQ=255)         tGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGA‑TTCTg                                                                                                                                   >  2:1354412/1‑140 (MQ=255)         tGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGA‑TTCTg                                                                                                                                   <  2:1549458/140‑1 (MQ=255)         tGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGGGAAGA‑TTCTg                                                                                                                                   >  1:161361/1‑140 (MQ=255)           aaaGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGGGAAGA‑ATTTGGc                                                                                                                                 >  2:743979/1‑140 (MQ=255)               cACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAGCCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGA‑TTCTGGCGGaa                                                                                                                             <  1:1802260/140‑1 (MQ=255)               cACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGA‑TTCTGGCGGaa                                                                                                                             <  1:508130/140‑1 (MQ=255)               cACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGA‑TTCTGGCGGaa                                                                                                                             <  1:597180/140‑1 (MQ=255)               cACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGGGAAGA‑TTCTGGCGGaa                                                                                                                             >  2:1513958/1‑140 (MQ=255)                  gcggcgTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCATAGCCGGAGAAAATTGAGGCGGGGGTGGAGAGTTCT‑GCGGAagag                                                                                                                          >  2:341692/1‑140 (MQ=255)                   cggcgTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCATAGCCGGAGAAAATTGAGGCGGGGGTGAAGA‑TTCTGGCGGAAgaga                                                                                                                         <  1:341692/140‑1 (MQ=255)                   cggcgTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGA‑TTCTGGCGGAAgaga                                                                                                                         <  1:1301883/140‑1 (MQ=255)                      cgTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGA‑TTCTGGCGGa                                                                                                                              >  2:1810414/1‑132 (MQ=255)                      cgTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGA‑TTCTGGCGGa                                                                                                                              <  1:1810414/132‑1 (MQ=255)                                       ggCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGGGAAGA‑TTCTGGCGGAAGAGATAGAAAGAGCCTGGGCTGaa                                                                                                     >  2:192858/1‑140 (MQ=255)                                       ggCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGGGAAGA‑TTCTGGCGGAAGAGATAGAAAAAGCCTGGGCTGaa                                                                                                     >  2:1354055/1‑140 (MQ=255)                                        gCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGA‑TTCTGGCGGAAGAGATAGAAAGAGCCTGGGCTGaaa                                                                                                    <  2:913532/140‑1 (MQ=255)                                                        gggCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGGGAAGA‑TTCTGGCGGAAGAGATAGAAAGAGCCTGGGCTGAAAGGCACTAAAAATTGcc                                                                                    >  2:354118/1‑140 (MQ=255)                                                          gCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGA‑TTCTGGCGGAAGAGATAGAAAGAGCCTGGGCTGAAAGTCACTAAAAATTGCCTg                                                                                  <  2:1155955/140‑1 (MQ=255)                                                           cTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGA‑TTCTGGCGGAAGAGATAGAAAGAGCCTGGGCTGAAAGTCACTAAAAATTGCCTGa                                                                                 <  1:657515/140‑1 (MQ=255)                                                              gATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGGGAAGA‑TTCTGGCGGAAGAGATAGAAAGAGCCTGGGCTGGAAGGCACTAAAAATTTCCTGATgc                                                                              >  2:1893515/1‑140 (MQ=255)                                                              gATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGGGAAGA‑TTCTGGCGGAAGAGATAGAAAGAGCCTGGGCTGAAAGTCACTAAAAATTGCCTGATgc                                                                              >  1:1629224/1‑140 (MQ=255)                                                               aTAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGA‑TTCTGGCGGAAGAGATAGAAAGAGCCTGGGCTGAAAGTCACTAAAAATTGCCTGATgcg                                                                             <  1:436660/140‑1 (MQ=255)                                                               aTAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGA‑TTCTGGCGGAAGAGATAGAAAGAGCCTGGGCTGAAAGTCACTAAAAATTGCCTGATgcg                                                                             <  1:1144938/140‑1 (MQ=255)                                                                  aaCCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGA‑TTCTGGCGGAAGAGATAGAAAGAGCCTGGGCTGAAAGTCACTAAAAATTGCCTGATGCGCTa                                                                          <  1:615703/140‑1 (MQ=255)                                                                   aCCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGA‑TTCTGGCGGAAGAGATAGAAAGAGCCTGGGCTGAAAGTCACTAAAAATTGCCTGATGCGCTa                                                                          >  2:597571/1‑139 (MQ=255)                                                                   aCCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGA‑TTCTGGCGGAAGAGATAGAAAGAGCCTGGGCTGAAAGTCACTAAAAATTGCCTGATGCGCTa                                                                          <  1:597571/139‑1 (MQ=255)                                                                   aCCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGGGAAGA‑TTCTGGCGGAAGAGATAGAAAGAGCCTGGGCTGAAAGTCACTAAAAATTGCCTGATGCGCTAc                                                                         >  2:1477883/1‑140 (MQ=255)                                                                    ccGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGA‑TTCTGGCGGAAGAGATAGAAAGAGCCTGGGCTGAAAGTCACTAAAAATTGCCTGATGCGCTACg                                                                        <  1:1354412/140‑1 (MQ=255)                                                                        ggCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGA‑TTCTGGCGGAAGAGATAGAAAGAGCCTGGGCTGAAAGTCACTAAAAATTGCCTGATGCGCTACGCTTa                                                                    >  1:993913/1‑140 (MQ=255)                                                                        ggCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGA‑TTCTGGCGGAAGAGATAGAAAGAGCCTGGGCTGAAAGGCACTAAAAATTGCCTGATGCGCTACGCTTa                                                                    >  2:32927/1‑140 (MQ=255)                                                                        ggCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGGGAAGA‑TTCTGGCGGAAGAGATAGAAAGAGCCTGGGCTGAAAGTCACTAAAAATTGCCTGATGCGCTACGCTTa                                                                    >  2:1603965/1‑140 (MQ=255)                                                                        ggCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGGGAAGA‑TTCTGGCGGAAGAGATAGAAAGAGCCTGGGCTGAAAGTCACTAAAAATTGCCTGATGCGCTACGCTTa                                                                    >  2:1057459/1‑140 (MQ=255)                                                                        ggCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGGGAAGA‑TTCTGGCGGAAGAGATAGAAAGAGCCTGGGCTGAAAGTCACTAAAAATCGCCTGATGCGCTACGCTTa                                                                    >  1:1184568/1‑140 (MQ=255)                                                                        ggCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGGGAAGA‑TTCTGGCGGAAGAGATAGAAAGAGCCTGGGCTGAAAAGCACTAAAAATTGCCTGATGGGCTACGCTTa                                                                    >  2:1944372/1‑140 (MQ=255)                                                                        ggCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGGGAAGA‑TTCTGGCGGAAGAGATAGAAAAAGCCCGGGCTGAAAGACACTAAAAATTGCCTGATGCGCCACGCttt                                                                    >  2:615703/1‑139 (MQ=255)                                                                         gCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGA‑TTCTGGCGGAAGAGATAGAAAGAGCCTGGGCTGAAAGTCACTAAAAATTGCCTGATGCGCTACg                                                                        >  1:1673911/1‑135 (MQ=255)                                                                         gCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGA‑TTCTGGCGGAAGAGATAGAAAGAGCCTGGGCTGAAAGTCACTAAAAATTGCCTGATGCGCTACg                                                                        <  2:1673911/135‑1 (MQ=255)                                                                         gCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGA‑TTCTGGCGGAAGAGATAGAAAGAGCCTGGGCTGAAAGTCACTAAAAATTGCCTGATGCGCTACGCTTAt                                                                   >  1:744900/1‑140 (MQ=255)                                                                         gCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGA‑TTCTGGCGGAAGAGATAGAAAGAGCCTGGGCTGAAAGTCACTAAAAATTGCCTGATGCGCTACGCTTAt                                                                   <  2:1629224/140‑1 (MQ=255)                                                                         gCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGA‑TTATGGCGGAAGAGATAGAAAGAGCCTGGGCTGAAAGTCACTAAAAATTGCCTGATGCGCTACGCTTAt                                                                   <  1:1604947/140‑1 (MQ=255)                                                                         gCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGGGAAGA‑TTCTGGCGGAAGAGATAGAAAGAGCCTGGGCTGAAAGTCACTAAAAATTGCCTGATGCGCTACGCTTAt                                                                   >  2:1093505/1‑140 (MQ=255)                                                                         gCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGGGAAGA‑TTATGGCGGGAAAGAAAAAAAAAGCCCGGGCTGGAAATTACCAAAAAATTGCTGATGCGCCAAGCtttt                                                                   >  2:1604947/1‑138 (MQ=255)                                                                            gCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGA‑TTCTGGCGGAAGAGATAGAAAGAGCCTGGGCTGAAAGTCACTAAAAATTGCCTGATGCGCTACGCTTATCAg                                                                <  1:1032681/140‑1 (MQ=255)                                                                             cATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGA‑TTCTGGCGGAAGAGATAGAAAGAGCCTGGGCTGAAAGTCACTAAAAATTGCCTGATGCGCTACGCTTATCAgg                                                               <  2:744900/140‑1 (MQ=255)                                                                             cATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGA‑TTCTGGCGGAAGAGATAGAAAGAGCCTGGGCTGAAAGTCACTAAAAATTGCCTGATGCGCTACGCTTATCAgg                                                               <  2:1647817/140‑1 (MQ=255)                                                                             cATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGA‑TTCTGGCGGAAGAGATAGAAAGAGCCTGGGCTGAAAGTCACTAAAAATTGCCTGATGCGCTACGCTTATCAgg                                                               <  1:192858/140‑1 (MQ=255)                                                                             cATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGA‑TTCTGGCGGAAGAGATAGAAAGAGCCTGGGCTGAAAGTCACTAAAAATTGCCTGATGCGCTACGCTTATCAgg                                                               <  1:1929550/140‑1 (MQ=255)                                                                              aTACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGA‑TTCTGGCGGAAGAGATAGAAAGAGCCTGGGCTGAAAGTCACTAAAAATTGCCTGATGCGCTACGCTTATCAGGc                                                              <  2:993913/140‑1 (MQ=255)                                                                              aTACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGGGAAGA‑TTCTGGCGGAAGAGATAGAAAGAGCCTGGGCTGAAAGTCACTAAAAATTGCCTGATGCGCTACGCTTATCAGGc                                                              >  2:1138489/1‑140 (MQ=255)                                                                                       aaTGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGA‑TTCTGGCGGAAGAGATAGAAAGAGCCTGGGCTGAAAGTCACTAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATGAt                                                     <  2:1352034/140‑1 (MQ=255)                                                                                        aTGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGA‑TTCTGGCGGAAGAGATAGAAAGAGCCTGGGCTGAAAGTCACTAAAAATTGCCTGATGCGCTACGCTTATCAgg                                                               <  2:394152/129‑1 (MQ=255)                                                                                        aTGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGGGAAGA‑TTCTGGCGGAAGAGATAGAAAGAGCCTGGGCTGAAAGTCACTAAAAATTGCCTGATGCGCTACGCTTATCAgg                                                               >  1:394152/1‑129 (MQ=255)                                                                                            aTGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGA‑TTCTGGCGGAAGAGATAGAAAGAGCCTGGGCTGAAAGTCACTAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATGAt                                                     >  1:727693/1‑135 (MQ=255)                                                                                            aTGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGA‑TTCTGGCGGAAGAGATAGAAAGAGCCTGGGCTGAAAGTCACTAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATGAt                                                     <  2:727693/135‑1 (MQ=255)                                                                                            aTGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGA‑TTCTGGCGGAAGAGATAGAAAGAGCCTGGGCTGAAAGTCACTAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATGATTTCTg                                                >  1:1101109/1‑140 (MQ=255)                                                                                             tGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGA‑TTCTGGCGGAAGAGATAGAAAGAGCCTGGGCTGAAAGTCACTAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATGATTTCTGc                                               <  1:336568/140‑1 (MQ=255)                                                                                              gcgcATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGA‑TTCTGGCGGAAGAGATAGAAAGAGCCTGGGCTGAAAGTCACTAAAAATTGCCTGATGCGCTACGCTTATCAg                                                                <  2:1515523/122‑1 (MQ=255)                                                                                              gcgcATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGA‑TTCTGGCGGAAGAGATAGAAAGAGCCTGGGCTGAAAGTCACTAAAAATTGCCTGATGCGCTACGCTTATCAg                                                                >  1:1515523/1‑122 (MQ=255)                                                                                              gcgcATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGA‑TTCTGGCGGAAGAGATAGAAAGAGCCTGGGCTGAAAGTCACTAAAAATTGCCTGATGCGCTACGCTTATCAGGcc                                                             >  2:895567/1‑125 (MQ=255)                                                                                              gcgcATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGA‑TTCTGGCGGAAGAGATAGAAAGAGCCTGGGCTGAAAGTCACTAAAAATTGCCTGATGCGCTACGCTTATCAGGcc                                                             <  1:895567/125‑1 (MQ=255)                                                                                              gcgcATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGA‑TTCTGGCGGAAGAGATAGAAAGAGCCTGGGCTGAAAGTCACTAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATGATTTCTGCa                                              <  1:1944372/140‑1 (MQ=255)                                                                                               cgcATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGA‑TTCTGGCGGAAGAGATAGAAAGAGCCTGGGCTGAAAGTCACTAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATGATTTCTGCaa                                             >  1:811205/1‑140 (MQ=255)                                                                                                   tGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGA‑TTCTGGCGGAAGAGATAGAAAGAGCCTGGGCTGAAAGTCACTAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATGATTTCTGCAatata                                         >  2:100709/1‑140 (MQ=255)                                                                                                   tGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGGGAAGA‑TTCTGGCGGAAGAGATAGAAAGAGCCTGGGCTGAAAGTCACTAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATGATTTCTGCAatata                                         >  1:1752291/1‑140 (MQ=255)                                                                                                   tGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGGGAAGA‑TTCTGGCGGAAGAGATAGAAAGAGCCTGGGCTGAAAGTCACTAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATGATTTCTGCAatata                                         >  2:1392395/1‑140 (MQ=255)                                                                                                   tGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGGGAAGA‑TTCTGGCGGAAGAGATAGAAAGAGCCTAGGCTGAAAAGCACTAAAAATTGCCTGATTCGCCACGCCTATTAGGCCTACATGATTTTTGCAatata                                         >  2:336568/1‑140 (MQ=255)                                                                                                     aaCTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGA‑TTCTGGCGGAAGAGATAGAAAGAGCCTGGGCTGAAAGTCACTAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATGATTTCTGCAATATAtt                                       >  2:1035355/1‑140 (MQ=255)                                                                                                     aaCTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGGGAAGA‑TTCTGGCGGAAGAGATAGAAAGAGCCTGGGCTGAAAGTCACTAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATGATTTCTGCAATATAtt                                       >  2:1291478/1‑140 (MQ=255)                                                                                                         aCGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGA‑TTCTGGCGGAAGAGATAGAAAGAGCCTGGGCTGAAAGTCACTAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATGATTTCTGCAATATATTGAAt                                   <  1:1093505/140‑1 (MQ=255)                                                                                                          cGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGA‑TTCTGGCGGAAGAGATAGAAAGAGCCTGGGCTGAAAGTCACTAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATGATTTCTGCa                                              >  1:1860424/1‑128 (MQ=255)                                                                                                          cGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGA‑TTCTGGCGGAAGAGATAGAAAGAGCCTGGGCTGAAAGTCACTAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATGATTTCTGCa                                              <  2:1860424/128‑1 (MQ=255)                                                                                                          cGTACCAGAGCCGGAGAAAATTGAGGCGGGGGGGAAGA‑TTCTGGCGGAAGAGATAGAAAGAGCCTGGGCTGAAAGTCACTAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATGATTTCTGCAATATATTGAAtt                                  >  2:1508567/1‑140 (MQ=255)                                                                                                            tACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGA‑TTCTGGCGGAAGAGATAGAAAGAGCCTGGGCTGAAAGTCACTAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATGATTTCTGCAATATATTGAATTTg                                <  1:1291478/140‑1 (MQ=255)                                                                                                            tACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGA‑TTCTGGCGGAAGAGATAGAAAGAGCCTGGGCTGAAAGTCACTAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATGATTTCTGCAATATATTGAATTTg                                <  1:1603965/140‑1 (MQ=255)                                                                                                            tACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGA‑TTCTGGCGGAAGAGATAGAAAGAGCCTGGGCTGAAAGTCACTAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATGATTTCTGCAATATATTGAATTTg                                <  2:681073/140‑1 (MQ=255)                                                                                                            tACCAGAGCCGGAGAAAATTGAGGCGGGGGGGAAGA‑TTCTGGCGGAAGAGATAGAAAGAGCCTGGGCTGAAAGGCACTAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATGATTTCTGCAATATATTGAATTTg                                >  2:617898/1‑140 (MQ=255)                                                                                                              ccAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGA‑TTCTGGCGGAAGAGATAGAAAGAGCCTGGGCTGAAAGTCACTAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATGATTTCTGCAATATATTGAATTTGga                              <  1:1138489/140‑1 (MQ=255)                                                                                                                    ccGGAGAAAATTGAGGCGGGGGTGAAGA‑TTCTGGCGGAATAGATAGAAAGAGCCTGGGCTGAAAGTCACTAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATGATTTCTGCAATATATTGAATTTgg                               >  1:1579318/1‑133 (MQ=255)                                                                                                                    ccGGAGAAAATTGAGGCGGGGGTGAAGA‑TTCTGGCGGAATAGATAGAAAGAGCCTGGGCTGAAAGTCACTAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATGATTTCTGCAATATATTGAATTTgg                               <  2:1579318/133‑1 (MQ=255)                                                                                                                    ccGGAGAAAATTGAGGCGGGGGCGAAGA‑TTCTGGCGGAAGAGATAGAAAGAGCCTGGGCTGAAAATCACTAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATGATTTCTGCAATATATTGAATTTGGAAGAAtt                        >  1:1330130/1‑140 (MQ=255)                                                                                                                     cGGAGAAAATTGAGGCGGGGGGGAAGA‑TTCTGGCGGAAGAGATAGAAAGAGCCTGGGTTGAAAGTCACTCAAAATTGCCTGATTCGCTACCCTTATCAGGCCTACATGATTTCTTCTATATATTTAATTTTGAAGTAttt                       >  2:1406737/1‑140 (MQ=38)                                                                                                                       gagaAAATTGAGGCGGGGGTGAAGA‑TTCTGGCGGAAGAGATAGAAAGAGCCTGGGCTGAAAGTCACTAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATGATTTCTGCAATATATTGAATTTGGAAGAATTTGt                     >  2:85729/1‑140 (MQ=255)                                                                                                                          aaaaTTGAGGCGGGGGTGAAGA‑TTCTGGCGGAAGAGATAGAAAGAGCCTGGGCTGAAAGTCACTAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATGATTTCTGCAATATATTGAATTTGGAAGAATTTGTAgg                  <  1:100709/140‑1 (MQ=255)                                                                                                                            aaTTGAGGCGGGGGTGAAGA‑TTCTGGCGGAAGAGATAGAAAGAGCCTGGGCTGAAAGTCACTAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATGATTTCTGCAATATATTGAATTTGGAAGAATTTGTAGGcc                <  1:1477883/140‑1 (MQ=255)                                                                                                                                gAGGCGGGGGTGAAGA‑TTCTGGCGGAAGAGATAGAAAGAGCCTGGGCTGAAAGTCACTAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATGATTTCTGCAATATATTGAATTTGGAAGAATTTGTAGGCCGGAt            <  1:1035355/140‑1 (MQ=255)                                                                                                                                      ggggggAAGA‑TTCTGGCGGAAGAGATAGAAAGAGCCTGGGCTGAAAGTCACTAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATGATTTCTGCAATATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCg      >  2:695609/1‑140 (MQ=255)                                                                                                                                       ggggGAAGA‑TTCTGGCGGAAGAGATAGAAAGAGCCTGGGCTGAAAGTCACTAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTAGATGATTTCTGCAATATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGt     >  2:102737/1‑140 (MQ=255)                                                                                                                                          tGAAGA‑TTCTGGCGGAAGAGATAGAAAGAGCCTGGGCTGAAAGTCACTAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATGATTTCTGCAATATATTGAATTTGGAAGAATTTGTAGGACGGATAAGGCGTTTa  <  1:1057459/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                             TCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGA‑TTCTGGCGGAAGAGATAGAAAGAGCCTGGGCTGAAAGTCACTAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATGATTTCTGCAATATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTA  >  NC_000913/3740772‑3741048

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑