Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,745,3970AC28.0% 124.9 / 10.8 75L198F (TTA→TTC) yiaN2,3‑diketo‑L‑gulonate:Na(+) symporter ‑ membrane subunit
Reads supporting (aligned to +/- strand):  ref base A (27/27);  new base C (0/21);  total (27/48)
Fisher's exact test for biased strand distribution p-value = 1.14e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 106 total aligned reads displayed.

TTCCATTCCGTTTATTATCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGC‑GGGCATTGCCCCTGGCATGATGATGGGCGCAACGCTGAT‑GCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGG‑AAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGCCTGTTTACCCCTACCGAAGCCGGGGCCGTTGC  >  NC_000913/3745259‑3745534
                                                                                                                                            |                                                                                                                                          
ttCCATTCCGTTTATTATCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGC‑GGGCATTGCCCCTGGCATGATGATGGGCGCAACGCTGAT‑GCTTACCTGGTGGGGGCAGGCCAGTCGCCTTAATTTAc                                                                                                                                           >  2:1372926/1‑140 (MQ=255)
ttCCATTCCGTTTATTATCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGC‑GGGCATTGCCCCCGGCATGATGATGGGCGCAACGCTGAT‑TCTTACCTGGTGGGGGCAGGCCAGTCGCCTTAATTTAc                                                                                                                                           >  2:139580/1‑140 (MQ=255)
 tCCATTCCGTTTATTATCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGC‑GGGCATTGCCCCTGGCATGATGATGGGCGCAACGCTGAT‑GCTTACCTGGTGGGGGCAGGCCAGTCGCCTTAATTTAcc                                                                                                                                          >  1:365132/1‑140 (MQ=255)
        cGTTTATTATCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGTTGTTTATGGC‑GGGCATTGCCCCTGGCATGATGATGGGCGCACCGCTGAT‑GCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTACCCCGCCAg                                                                                                                                   <  1:1261954/140‑1 (MQ=255)
        cGTTTATTATCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGC‑GGGCATTGCCCCCGGCATGATGATGGGCGCAACCCCGAT‑GCTTACCTGGTGGTGGCAGGCCAGGCGCCTTAATTTACcccccccc                                                                                                                                   >  2:1052263/1‑138 (MQ=255)
          tttATTATCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGC‑GGGCATTTCCCCTGGGGTGAAGATGGGAGCAACGCTGAT‑GCTTACCTTGTGGTGGCAGGGCCCACGCCTTAATTTACCCCCCCAACa                                                                                                                                 >  2:1261954/1‑140 (MQ=255)
          tttATTATCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGC‑GGGCATTGCCCCTGGCCTGGTGATGGGCGCAACGCTGAT‑GCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTACCCCGCCCGCa                                                                                                                                 >  1:1389593/1‑140 (MQ=255)
          tttATTATCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGC‑GGGCATTGCCCCTGGCATGCTGATGGGCGCAACCCTGAT‑GCTTACCCCGGGGGGGCAGGCCAGCCGCCTTTATTTACCCCCCCCGCa                                                                                                                                 >  2:1533205/1‑140 (MQ=255)
          tttATTATCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGC‑GGGCATTGCCCCTGGCATGATGATGGGCGCAACGCTGAT‑GCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTACCCCGCCAGCa                                                                                                                                 <  2:365132/140‑1 (MQ=255)
          tttATTATCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGC‑GGGCATTGCCCCCCGCATGATGGTGGGGGGAACGCTGAT‑GATTAACTCGTGGTGGCAGGCCCGTAGCCTTAATTTACCCCGCCAGCa                                                                                                                                 >  2:1160346/1‑140 (MQ=255)
            tattatCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGC‑GGGCATTGCCCCCGGCATGATGATGGGCGCAACGCTGAT‑GCTTACCTGGGGGGGGCCGGGCAGGCGCCCTAATTTACCCCCCCCGCaaa                                                                                                                               >  2:244381/1‑140 (MQ=255)
                   ttCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGC‑GGGCATTGCCCCTGGCATGATGATGGGCGCAACGCTGAT‑GCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTACCCCGCCAGCAAAAAGCAAc                                                                                                                        <  1:1765641/140‑1 (MQ=255)
                   ttCGGCGTTTCCAGCGGATTATCAATCAGTAAGCGGTTTATGGC‑GGGCTTTGCCCCTGGCATGATGATGGGCGCACCGCTGAT‑GCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTACCCCGCCAGCAAAAAGCAAc                                                                                                                        <  1:2003691/140‑1 (MQ=255)
                    tCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGGGATTG‑CCCTGGCCTGCTGATGGGCGCAACACTTAG‑TCTTACCTGGTGGGGGGGGGGCGGACGTCTTAATTTACCCCCCCAGCAAAAAACAACg                                                                                                                       >  2:2081777/1‑140 (MQ=255)
                    tCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGC‑GGGGATTGTCCCCGGCGTGATGATGGGCGGCAAGCTTGT‑GCTTTCCTGGTGGGGGCAGGGCAGTCGCCTTTATTTACCCCCCCAGCAAAAAGCAACg                                                                                                                       >  2:1100967/1‑140 (MQ=255)
                    tCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGC‑GGGCATTTTCCCTGGCATGCTGAGGGGGGCCACCCTGAT‑GTTTTCCCCGGGGGGGCCGGTCCGGCTCCTTTATTTTCCCCCCCCTCCAAAACCAAca                                                                                                                       >  2:778300/1‑139 (MQ=255)
                    tCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGC‑GGGCATTGTCCCTGGCATGATGATGGGCGCAACGCTGAT‑GCTTACCTGGTGGGGGGAGGCCAGTCGCCTTAATTTACCCCCCCCGCAAAAAACAACg                                                                                                                       >  2:583450/1‑140 (MQ=255)
                    tCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGC‑GGGCATTGCCCCTGGCATGATGATGGGGGCAACGCTGAT‑GCTTACCTGGTGGGGGCAGGCCCGTCGCCCTAATTTACCCCGCCCGCAAAAAGCAACg                                                                                                                       >  2:1929430/1‑140 (MQ=255)
                    tCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGC‑GGGCATTGCCCCTGGCATGATGATGGGCGCAACGCTGAT‑GCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTACCCCCCCCGCAAAAaacaacc                                                                                                                       >  2:1648775/1‑139 (MQ=255)
                    tCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGC‑GGGCATTGCCCCCGGCATGATGATGGGGGCCACGCTGAT‑GCTTTCCTGGTGGTGGCAGGGCCGTCGCCTTAATTTTCCCCCCCCGCAAAAAGCAACg                                                                                                                       >  2:738944/1‑140 (MQ=255)
                      ggCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGC‑GGGCATTGCCCCTGGCATGATGATGGGCGCAACGCTGAT‑GCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTCCCCCGCCAGCAAAAAGCaa                                                                                                                         <  2:1042992/136‑1 (MQ=255)
                      ggCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGC‑GGGCATTGCCCCTGGCATGATGATGGGCGCAACGCTGAT‑GCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTACCCCGCCAGCAAAAAGCaa                                                                                                                         >  1:1042992/1‑136 (MQ=255)
                         gTTTCCAGGGGATTATCCATCAGTAAGCTGTTTATGGC‑GGGCATTGCCCCTGGCATGATGATGGGCGCAACGCTGAT‑GCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCa                                                                                                                  <  1:1648775/140‑1 (MQ=255)
                         gTTTCCAGCGGATTTTCCATCAGTAAGCTGTTTATGGC‑GGGCATTGCCCCTGGCATGATGATGGGCGCAACGCTGAT‑GCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCa                                                                                                                  <  1:778300/140‑1 (MQ=255)
                           ttCCAGCGGATTATCCATCAGTAAGCTGTTTATGGC‑GGGCATTTCCCCCGGCCTGATGATGGGCGCAACGCTGAT‑GCTTTCCTGGTGGTGGGAGGGCAGTCGCCTTTATTTACCCCCCCAGCCAAAAACAACGATGCAgg                                                                                                                >  2:641221/1‑140 (MQ=255)
                             ccAGCGGATTATCCATCAGTAAGCTGTTTATGGC‑GGGCATTGCCCCTGGCATGATGATGGGCGCAACGCTGAT‑GCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGG‑aa                                                                                                             <  1:1477728/140‑1 (MQ=255)
                                    aTTATCCATCAGTAAGCTGTTTATGGC‑GGGCATTGCCCCTGGCCTGATGATGGGCGCAAAGCTGAT‑GCTTACCCGGTGGTGGCAGGCCAGTCGCCCTAATTTACCCCCCCCGCAAAAAACAACGATGCAGG‑AAAACTGGc                                                                                                      >  2:1658330/1‑140 (MQ=255)
                                    aTTATCCATCAGTAAGCTGTTTATGGC‑GGGCATTGCCCCTGGCATGATGATGGGCGCAACGCTGAT‑GCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTCCCCCGCCAGCAAAAAGCAACGATGCAGG‑AAATCTGGc                                                                                                      <  1:139580/140‑1 (MQ=255)
                                      tATCCATCAGTAAGCTGTTTATGGC‑GGGCATTGCCCCTGGCATGATGATGGGCGCAACGCTGAT‑GCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTCCCCCGCCAGCAAAAAGCAACGATGCAGG‑AAATCTGGCAc                                                                                                    <  2:190963/140‑1 (MQ=255)
                                      tATCCATCAGTAAGCTGTTTATGGC‑GGGCATTGCCCCTGGCATGATGATGGGCGCAACGCTGAT‑GCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGG‑AAATCTGGCAc                                                                                                    <  1:1627818/140‑1 (MQ=255)
                                          cATCAGTAAGCTGTTTATGGC‑GGGCATTGCCCCTGGCATGATGATGGGCGCAACGCTGAT‑GCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGG‑AAATCTGGCACTCtt                                                                                                <  1:1658330/140‑1 (MQ=255)
                                            tCAGTAAGCTGTTTATGGC‑GGGCATTGCCCCTGGCATGATGATGGGCGCAACGCTGAT‑GCTTCCCTGGTGGTGGCAGGCCAGTCGCCTTAATTTCCCCCGCCAGCAAAAAGCAACGATGCAGG‑AAATCTGGCACTCtttt                                                                                              <  1:1469852/140‑1 (MQ=255)
                                             cAGTAAGCTGTTTATGGC‑GGGCATTGCCCCTGGCATGATGATGGGCGCAACGCTGAT‑GCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGG‑AAATCTGGCACTCTTTTg                                                                                             <  1:1302975/140‑1 (MQ=255)
                                                 aaGCTGTTTATGGG‑GGGCATTGCCCCTGGCATGATGAGGGGCGCAACGCTGAT‑GCTTACCTGGTGGTGGCAGGCCAGTCGCCTTATTTTACCCCGCCAGCAAAAAGCAACGATGCAGG‑AAATCTGGCACTCTTTTGtctc                                                                                         <  2:138985/140‑1 (MQ=255)
                                                 aaGCTGTTTATGGC‑GGGCATTGCCCCTGGCATGATGATGGGCGCAACGCTGAT‑GCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTCCCCCGCCAGCAAAAAGCAACGATGCAGG‑AAATCTGGCACTCTTTTGtctc                                                                                         <  2:100297/140‑1 (MQ=255)
                                                 aaGCTGTTTATGGC‑GGGCATTGCCCCTGGCATGATGATGGGCGCAACGCTGAT‑GCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTCCCCCGCCAGCAAAAAGCAACGATGCAGG‑AAATCTGGCACTCTTTTGtctc                                                                                         <  1:1929430/140‑1 (MQ=255)
                                                 aaGCTGTTTATGGC‑GGGCATTGCCCCTGGCATGATGATGGGCGCAACGCTGAT‑GCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTCCCCCGCCAGCAAAAAGCAACGATGCAGG‑AAATCTGGCACTCTTTTGtctc                                                                                         <  2:1658585/140‑1 (MQ=255)
                                                 aaGCTGTTTATGGC‑GGGCATTGCCCCTGGCATGATGATGGGCGCAACGCTGAT‑GCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGG‑AAATCTGGCACTCTTTTGtctc                                                                                         <  2:816265/140‑1 (MQ=255)
                                                  aGCTGTTTATGGC‑GGGCATTGCCCCTGGCCTGATGGTGGGCGCCGCGCCGAT‑GCTTACCCGGTGGGGGGCGGGCAGTCGCCTTTATTTACCCCGCCAGCAAAAAGCAAGGACGCAGG‑AAATCTTGCACTCTTTTTTTTcc                                                                                        >  2:1244034/1‑140 (MQ=255)
                                                  aGCTGTTTATGGC‑GGGCATTGCCCCCGGCATGATGATGGGGGCAACGCTGAT‑GCTTTCCTGGTGGTGGGAGGCCAGGCGCCTTAATTTTCCCCCCCCGCAAAAAACAACGATGCAGG‑GAATCTGGCACTCTTTTTTCTcc                                                                                        >  2:1989174/1‑140 (MQ=255)
                                                  aGCTGTTTATGGC‑GGGCATTGCCCCCGGCATGATGATGGGGGCAACGCTGAT‑GCTTACCTGGTGGGGGCAGGCCAGTCGCCTTAATTTACCCCCCCAGCAAAAAACAACGATGCAGG‑AAATCTGGCACTCTTTTGTCTcc                                                                                        >  2:1156388/1‑140 (MQ=255)
                                                     tGTTTATGGC‑GGGCATTGCCCCCGGCATGATGATGGGGGCAACGCTGAT‑GCTTACCTGGTGGGGGGAGGCCAGTCGCCTTAATTTACCCCCCCCGCAAAAAACAACGATGCAGG‑AAATCTGGGACTCTTTTTTCTCCggg                                                                                     >  2:541109/1‑139 (MQ=255)
                                                       tttATGGC‑GGGCATTTCCCCTGGCATGATGATGGGCGCAACGCTGAT‑GCTTTCCTGGTGGGGGCAGGCCAGTCGCCTTTATTTACCCCCCCCGCAAAAAACAACGATGCAGG‑AAATCTGGCACTCTTTTTTCTCCGGGAt                                                                                   >  2:1158179/1‑140 (MQ=255)
                                                       tttATGGC‑GGGCATTGCCCCTGGCATGATGATGGGCGCAACGCTGAT‑GCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGG‑AAATCTGGCACTCTTTTGTCTCCGGTAt                                                                                   >  2:908112/1‑140 (MQ=255)
                                                       tttATGGC‑GGGCATTGCCCCTGGCATGATGATGGGCGCAACGCTGAT‑GCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGG‑AAATCTGGCACTCTTTTGTCTCCGGTAt                                                                                   >  1:1740350/1‑140 (MQ=255)
                                                       tttATGGC‑GGGCATTGCCCCTGGCATGATGATGGGCGCAACGCTGAT‑GCTTACCTGGTGGGGGCAGGCCAGTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGG‑AAATCTGGCACTCTTTTGTCTCCGGTAt                                                                                   >  1:1458918/1‑140 (MQ=255)
                                                       tttATGGC‑GGGCATTGCCCCTGGCATGATGATGGGCGCAACGCTGAT‑GCTTACCTGGTGGGGGCAGGCCAGTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGG‑AAATCTGGCACTCTTTTGTCTCCGGTAt                                                                                   >  1:2192493/1‑140 (MQ=255)
                                                         tATGGC‑GGGCATTGCCCCTGGCATGATGATGGGCGCACCGCTGAT‑GCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGG‑AAATCTGGCACTCTTTTGTCTCCGGTATCt                                                                                 <  2:1389593/140‑1 (MQ=255)
                                                         tATGGC‑GGGCATTGCCCCTGGCATGATGATGGGCGCAACGCTGAT‑GCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTCCCCCGCCAGCAAAAAGCAACGATGCAGG‑AAATCTGGCACTCTTTTGTCTCCGGTATCt                                                                                 <  1:1533205/140‑1 (MQ=255)
                                                         tATGGC‑GGGCATTGCCCCTGGCATGATGATGGGCGCAACGCTGAT‑GCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGG‑AAATCTGGCACTCTTTTGTCTCCGGTATCt                                                                                 <  1:2026316/140‑1 (MQ=255)
                                                            ggC‑GGGCATTGCCCCTGGCATGATGATGGGGGCAACGCTGAT‑GCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTACCCCGCCCGCAAAAAACAACGATGCAGG‑AAATCTGGCACTCTTTTTTCTCCGGGATCTggg                                                                              >  2:262132/1‑140 (MQ=255)
                                                             gC‑GGGCATTGCCCCTGGCATGATGATGGGCGCAACGCTGAT‑GCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTCCCCCGCCAGCAAAAAGCAACGATGCAGG‑AAATCTGGCACTCTTTTGTCTCCGGTATCTGGgc                                                                             <  1:541109/140‑1 (MQ=255)
                                                             gC‑GGGCATTGCCCCTGGCATGATGATGGGCGCAACGCTGAT‑GCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGG‑AAATCTGGCACTCTTTTGTCTCCGGTATCTGGgc                                                                             <  1:1936393/140‑1 (MQ=255)
                                                              c‑GGGCATTGCCCCTGGCATGATGATGGGCGCAACGCTGAT‑GCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTCCCCCGCCAGCAAAAAGCAACGATGCAGG‑AAATCTGGCACTCTTTTGTCTCCGGTATCTGGgcg                                                                            <  1:1158179/140‑1 (MQ=255)
                                                              c‑GGGCATTGCCCCTGGCATGATGATGGGCGCAACGCTGAT‑GCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTCCCCCGCCAGCAAAAAGCAACGATGCAGG‑AAATCTGGCACTCTTTTGTCTCCGGTATCTGGgcg                                                                            <  1:1031737/140‑1 (MQ=255)
                                                               ggggCATTGCCCCTGGCATGATGATGGGCGCAACGCTGAT‑GTTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTCCCCCGCCAGCAAAAAGCAACGATGCAGG‑AAATCTGGCACTCTTTTGTCTCCGGTATCTGGgcg                                                                            <  1:2124427/139‑1 (MQ=255)
                                                               ggggCATTGCCCCTGGCATGATGATGGGCGCAACGCTGAT‑GCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGG‑AAATCTGGCACTCTTTTGTCTCCGGTATCTGGgcg                                                                            <  1:583450/139‑1 (MQ=255)
                                                                 ggCATTGCCCCTGGCATGATGATGGGCGCAACGCTGAT‑GCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTCCCCCGCCAGCAAAAAGCAACGATGCAGG‑AAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCt                                                                          <  1:1372926/140‑1 (MQ=255)
                                                                 ggCATTGCCCCTGGCATGATGATGGGCGCAACGCTGAT‑GCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGG‑AAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCt                                                                          <  2:1557838/140‑1 (MQ=255)
                                                                  gccttGCCCCCGGCGTGATGAGGGGCGCCACGCCGATGGCTTCCCTGG‑GGTGGGAGGCCAGTCGCCTTAATTTAACCCCCCAGGAAAAAGCAACGAAGCCGGAAAAACTGG‑ACTCTTTTGTCTTCGGGATCTGGGCGCTg                                                                         >  2:537381/4‑140 (MQ=255)
                                                                  gCATTTCCCCCTGCATGAAGGCGGGCGCAACGCCGAG‑GATTTCCTGGTGGGGGCAGGCCAGTCGGCTTAATTTTTCCCGCCAGCAAAAAGCAACAATGCAGG‑AAAACTGGCACTCTTTTTTCTCCGGCATCTTGGCGCTg                                                                         >  2:1353616/1‑140 (MQ=255)
                                                                  gCATTTCCCCCGGGATGATGATGGGCGCAACGCTGAT‑GCTTACCTGGTGGGGGGAGGCCCGCCGCCTTAATTTTCCCCGCCAGCAAAAAAAAACGATGCAGG‑AAAAATGGCACTCTTTTGTCTCCGGGATCTTGGCGCTg                                                                         >  2:2124427/1‑140 (MQ=255)
                                                                  gCATTGCCCCTGGCATGATGATGGGCGCAACGCTGAT‑GCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGG‑AAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTg                                                                         <  2:689370/140‑1 (MQ=255)
                                                                  gCATTGCCCCTGGCATGATGATGGGCGCAACGCTGAT‑GCTTACCTGGTGGTGGCAGGACAGTCGCCTTAATTTCCCCCGCCAGCAAAAAGCAACGATGCAGG‑AAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCt                                                                          <  1:1784973/139‑1 (MQ=255)
                                                                  gCATTGCCCCTGGCATGATGATGGGCGCAACGCTGAT‑GCTTACCTGGTGGTGGCAGGACAGTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGG‑AAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCt                                                                          >  2:1784973/1‑139 (MQ=255)
                                                                  gCATTGCCCCCGGCATGATGATGGGCGCCACGCTGGT‑GCTTACCTGGGGGTGGGAGGCCAGGCGCCTTAATTTTCCCCCCCCGCAAAAAGCAACGATGCAGG‑AAATCTGGCACTCTTTTTTTTCCCGTATTTGGGCGCTg                                                                         >  2:1031737/1‑140 (MQ=255)
                                                                      tGCCCCTGGCATGATGATGGGCGCAACGCTGAT‑GCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGG‑AAATCTGGCACTCTTTTGTCTCCGGTATCTGGgcg                                                                            >  1:1722234/1‑133 (MQ=255)
                                                                      tGCCCCTGGCATGATGATGGGCGCAACGCTGAT‑GCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGG‑AAATCTGGCACTCTTTTGTCTCCGGTATCTGGgcg                                                                            <  2:1722234/133‑1 (MQ=255)
                                                                       gCCCCTGGCATGATGATGGGCGCAACGCTGAT‑GCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCATG‑AAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTttct                                                                    <  1:1160346/140‑1 (MQ=255)
                                                                           cTGGCATGATGATGGGCGCAACGCTGAT‑GCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTCCCCCGCCAGCAAAAAGCAACGATGCAGG‑AAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCa                                                                <  2:2012551/140‑1 (MQ=255)
                                                                              gCATGATGATGGGCGCAACGCTGAT‑GCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTCCCCCGCCAGCAAAAAGCAACGATGCAGG‑AAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGtc                                                             <  1:908112/140‑1 (MQ=255)
                                                                                  gatgatgGGCGCAACGCTGAT‑GCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGG‑AAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGtt                                                                       >  2:1436773/1‑126 (MQ=255)
                                                                                  gatgatgGGCGCAACGCTGAT‑GCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGG‑AAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGtt                                                                       <  1:1436773/126‑1 (MQ=255)
                                                                                  gatgatgGGCGCAACGCTGAT‑GCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGG‑AAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGtcatca                                                         >  1:479084/1‑140 (MQ=255)
                                                                                   atgatgGGCGCAACGCTGAT‑GCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGG‑AAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGtcatc                                                          <  2:1302617/138‑1 (MQ=255)
                                                                                   atgatgGGCGCAACGCTGAT‑GCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGG‑AAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGtcatc                                                          >  1:1302617/1‑138 (MQ=255)
                                                                                   atgatgGGCGCAACGCTGAT‑GCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGG‑AAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTcatcat                                                        >  2:1297531/1‑140 (MQ=255)
                                                                                         ggCGCAACGCTGAT‑GCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGG‑AAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTgg                                                  >  2:1878083/1‑140 (MQ=255)
                                                                                         ggCGCAACGCTGAT‑GCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGG‑AAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTgg                                                  >  1:509424/1‑140 (MQ=255)
                                                                                             cAACGCTGAT‑GCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGG‑AAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCggg                                              >  1:1350299/1‑140 (MQ=255)
                                                                                              aaCGCTGAT‑GCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGG‑AAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGt                                             >  1:1857188/1‑140 (MQ=255)
                                                                                                    gAT‑GCTTACCTGGTGGTGGCAGGCCAGTCCCCTTAATTTCCCCCGCCAGCAAAAAGCAACGATGCAGG‑AAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCt                                       <  2:1152582/140‑1 (MQ=255)
                                                                                                    gAT‑GCTTACCTGGTGGTGGCAGCCCAGTCGCTTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGG‑AAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCt                                       <  1:2081777/140‑1 (MQ=255)
                                                                                                         gttACCTGGTGGGGGCGGGCCAGTCGCCTTAATTTCCCCCGCCAGCAAAAAGCAACGATGCAGG‑AAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCgg                                   <  2:851974/139‑1 (MQ=255)
                                                                                                          ttACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTCCCCCGCCAGCAAAAAGCAACGATGCAGG‑AAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGc                                  <  2:479084/140‑1 (MQ=255)
                                                                                                          ttACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGG‑AAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGc                                  <  1:738944/140‑1 (MQ=255)
                                                                                                          ttACCTGGTGGTGGCAGGCCAGCCGCCTTAATTTCCCCCGCCAGCAAAAAGCAACGATGCAGG‑AAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGc                                  <  1:537381/140‑1 (MQ=255)
                                                                                                           tACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGG‑AAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGcc                                 >  1:1689671/1‑140 (MQ=255)
                                                                                                            aCCTGGTGGTGGCAGGCCAGTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGG‑AAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGGTTCGCTCCCGCCt                                >  2:1732401/1‑140 (MQ=255)
                                                                                                                   ggtggCAGGCCAGTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGG‑AAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGGTTCGCTCCGGCCCGTTTTcc                         >  2:1793832/1‑140 (MQ=255)
                                                                                                                    gtggCAGGCCAGTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGG‑AAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGCCTGTTTAccc                        >  1:281275/1‑140 (MQ=255)
                                                                                                                     tggCAGGCCAGTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGG‑AAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTTGCTCCTGCCTGTTTTcccc                       >  1:851974/1‑140 (MQ=255)
                                                                                                                      ggCAGGCCAGTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGG‑AAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGc                                  <  2:537511/128‑1 (MQ=255)
                                                                                                                      ggCAGGCCAGTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGG‑AAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGc                                  >  1:537511/1‑128 (MQ=255)
                                                                                                                       gCAGGCCAGTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGG‑AAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGGTTTGCTCCGGCCCGTTTAcccccc                     >  2:1163527/1‑138 (MQ=255)
                                                                                                                         aGGCCAGTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGG‑AAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGCCTGTTTACCCCTAcc                   <  2:1350299/140‑1 (MQ=255)
                                                                                                                           gCCAGTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGG‑AAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGGTTTGCTCCGGCCTGTTTACCCCTAccca                 >  1:1788736/1‑138 (MQ=255)
                                                                                                                           gCCAGTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGG‑AAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGGTTCGCTCCCGCCTGTTTTCCCCAAcccg                 >  2:264060/1‑138 (MQ=255)
                                                                                                                                tCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGG‑AAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGGTTTGCTCCGGCCTGTTTACCCCTACCCAAGccc            >  1:155314/1‑139 (MQ=255)
                                                                                                                                          ttACCCCGCCAGCAAAAAGCAACGATGCAGG‑AAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGCCTGTTTACCCCTACCGAAGCCGGGGCCGTtgc  <  2:1689671/140‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                          
TTCCATTCCGTTTATTATCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGC‑GGGCATTGCCCCTGGCATGATGATGGGCGCAACGCTGAT‑GCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGG‑AAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGCCTGTTTACCCCTACCGAAGCCGGGGCCGTTGC  >  NC_000913/3745259‑3745534

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑