Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,429,9840TG27.8% 164.1 / 43.1 97L41V (TTG→GTG) cycAserine/alanine/glycine/cycloserine:H(+)symporter
Reads supporting (aligned to +/- strand):  ref base T (10/60);  new base G (27/0);  total (37/60)
Fisher's exact test for biased strand distribution p-value = 4.72e-16
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 125 total aligned reads displayed.

GGTACAGGAAGAAAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATGCGGGCAATGGGGGAATTGCTGCTTTCGAATCTGGAATACAAATCTTT  >  NC_000913/4429845‑4430123
                                                                                                                                           |                                                                                                                                           
ggTACAGGAAGAAAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGgt                                                                                                                                             >  2:854304/1‑140 (MQ=255)
ggTACAGGAAGAAAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGgt                                                                                                                                             <  2:2128665/140‑1 (MQ=255)
ggTACAGGAAGAAAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGgt                                                                                                                                             <  1:425842/140‑1 (MQ=255)
ggTACAGGAAGAAAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGgt                                                                                                                                             <  1:2084873/140‑1 (MQ=255)
ggTACAGGAAGAAAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGgt                                                                                                                                             <  2:1790966/140‑1 (MQ=255)
ggTACAGGAAGAAAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGgt                                                                                                                                             <  2:838303/140‑1 (MQ=255)
 gTACAGGAAGAAAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGgtt                                                                                                                                            <  1:432802/140‑1 (MQ=255)
  tACAGGAAGAAAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCCTTGGTACGGGggtg                                                                                                                                           >  1:2133500/1‑140 (MQ=255)
  tACAGGAAGAAAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGgttg                                                                                                                                           >  2:612665/1‑140 (MQ=255)
  tACAGGAAGAAAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGgttg                                                                                                                                           >  2:1716008/1‑140 (MQ=255)
  tACAGGAAGAAAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGgttg                                                                                                                                           >  2:782650/1‑140 (MQ=255)
  tACAGGAAGAAAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGgttg                                                                                                                                           >  1:1440103/1‑140 (MQ=255)
       gaagaaAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGTGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTGTTTAt                                                                                                                                      >  2:330108/1‑140 (MQ=255)
       gaagaaAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCCTTGGGTTGGGGGTGttttt                                                                                                                                      >  2:471500/1‑138 (MQ=255)
        aagaaAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTGTTTATg                                                                                                                                     <  2:328239/140‑1 (MQ=255)
        aagaaAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTGTTTATg                                                                                                                                     <  2:1440103/140‑1 (MQ=255)
         agaaAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTGTTTAt                                                                                                                                      <  2:245586/138‑1 (MQ=255)
         agaaAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTGTTTATgg                                                                                                                                    >  1:1365119/1‑140 (MQ=255)
         agaaAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGGTGTTTTTgg                                                                                                                                    >  2:2085702/1‑140 (MQ=255)
           aaaaaaaCATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGGTGTTTATgggg                                                                                                                                  >  1:1182265/1‑140 (MQ=255)
           aaaaaaaCATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGGGCCCTTTGTACGGGGGTGTTTTTtggg                                                                                                                                  >  2:1894023/1‑140 (MQ=255)
            aaaaaaCATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTGTTTATGGGGt                                                                                                                                 <  1:1978169/140‑1 (MQ=255)
             aaaaaCATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTGTTTATGGGGTc                                                                                                                                <  1:301079/140‑1 (MQ=255)
               aaaCATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTGTTTATGGGGTCTg                                                                                                                              >  1:1832230/1‑140 (MQ=255)
                  cATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGGTGGTTATGGGGTGTGGCa                                                                                                                           >  2:1818586/1‑140 (MQ=255)
                   aTGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTGTTTATGGGGTCTGGCaa                                                                                                                          <  2:1552075/140‑1 (MQ=255)
                     ggTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGGTGTTTATGGGGTCTGGCaaaa                                                                                                                        >  1:1651544/1‑140 (MQ=255)
                        aGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGa                                                                                                                     <  2:1342789/140‑1 (MQ=255)
                        aGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGa                                                                                                                     <  1:2084197/140‑1 (MQ=255)
                            cAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGGTGTTTATGGGGTCTGGCAAAACGATTAg                                                                                                                 >  1:1906890/1‑140 (MQ=255)
                              ggTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCCTTGGTACGGGGGTGTTTTTGGGGGCTGGCAAAACGATTAGcc                                                                                                               >  2:171223/1‑140 (MQ=255)
                               gTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTTGTACGGGGGTGTTTTTGGGGTCTGGCAAAACGATTAGCCt                                                                                                              >  2:1656266/1‑140 (MQ=255)
                               gTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTTGTACGGGGGTGTTTTTGGGGTCTGGCAAAAAGATTAGCCt                                                                                                              >  2:1846278/1‑140 (MQ=255)
                               gTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGGTGTTTTTGGGGGCTGGCAAAACGATTAGCCt                                                                                                              >  2:1763660/1‑140 (MQ=255)
                               gTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGGTGTTTATTGGGGCTGGCAAAAAGATTAGCCt                                                                                                              >  2:77800/1‑140 (MQ=255)
                                 aaaaGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCCTTGGGACGGGGGTGTTTATGGGGTCTGGCAAAACGATTAGCCttt                                                                                                            >  2:1825444/1‑139 (MQ=255)
                                 aaaaGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGGTGTTTATGGGGTCTGGCAAAACGATTAGCCTTg                                                                                                            >  1:1230417/1‑140 (MQ=255)
                                 aaaaGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGGACGGGGGTGTTTTTTGGGTCTGGCAAAACGATTAGCCttt                                                                                                            >  2:1259354/1‑139 (MQ=255)
                                  aaaGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTGTTTATGGGGTCTGGC‑AAACGATTAGCCTTGcc                                                                                                          <  1:1248762/140‑1 (MQ=255)
                                    aGTCGTTGCCGATGATCCGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGGTGTTTATGGGGGCTGGCAAAACGATTAGCCTTGCCg                                                                                                         >  1:588508/1‑140 (MQ=255)
                                        gTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGcc                                                                                                     <  1:1588526/140‑1 (MQ=255)
                                         ttGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGTCATTGGCGGTGCCATTGGTACGGGGGTGTTTTTGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCg                                                                                                    >  1:1169964/1‑140 (MQ=255)
                                         ttGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCg                                                                                                    <  2:1182265/140‑1 (MQ=255)
                                         ttGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCg                                                                                                    >  1:730331/1‑140 (MQ=255)
                                            ccGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCg                                                                                                 <  2:1832230/140‑1 (MQ=255)
                                              gatgatCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGAt                                                                                               <  2:1725508/140‑1 (MQ=255)
                                                tgatCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCa                                                                                             >  1:1220017/1‑140 (MQ=255)
                                                  atCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCAtt                                                                                           <  1:171223/140‑1 (MQ=255)
                                                  atCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCAtt                                                                                           <  2:1365119/140‑1 (MQ=255)
                                                  atCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCAtt                                                                                           <  2:415959/140‑1 (MQ=255)
                                                    cAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCAtttt                                                                                         <  1:471500/140‑1 (MQ=255)
                                                      ggCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCAttt                                                                                          >  1:319170/1‑137 (MQ=255)
                                                      ggCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCg                                                                                       <  2:1854865/140‑1 (MQ=255)
                                                       gCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGt                                                                                      <  1:612665/140‑1 (MQ=255)
                                                       gCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGt                                                                                      <  2:1663829/140‑1 (MQ=255)
                                                        cTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGGACGGGGGTGTTTATGGGGGCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGtt                                                                                     >  1:1737186/1‑140 (MQ=255)
                                                           cGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTtat                                                                                  <  1:1410233/140‑1 (MQ=255)
                                                           cGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTtat                                                                                  <  2:1473069/140‑1 (MQ=255)
                                                            ggCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTtata                                                                                 <  1:1304104/140‑1 (MQ=255)
                                                            ggCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTtata                                                                                 <  1:1656266/140‑1 (MQ=255)
                                                            ggCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTtata                                                                                 <  1:1763660/140‑1 (MQ=255)
                                                            ggCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGGACGGGGGTGTTTATGGGGGCTGGCAAAACGATTAGCCTTTCCGGGCCGTCGATCATTTTCGTTtata                                                                                 >  1:2091952/1‑140 (MQ=255)
                                                             gCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCCTTTGTACGGGGGTGTTTTTGGGGGCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGCTCATTTTTGTTTttat                                                                                >  2:133499/1‑140 (MQ=255)
                                                             gCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCCTTGGTACGGGGGTGTTTTTGGGGTCTGGCAAAACGATTAGCCTTGCCGGGGCGTCGGTCATTTTTGTTTttat                                                                                >  2:163635/1‑140 (MQ=255)
                                                             gCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCTTCGATCATTTTCGTTTatat                                                                                <  1:1259354/140‑1 (MQ=255)
                                                             gCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTatat                                                                                <  1:1825444/140‑1 (MQ=255)
                                                              cTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATg                                                                               <  1:1225769/140‑1 (MQ=255)
                                                                 aaCAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGGTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATc                                                                            >  1:1779612/1‑140 (MQ=255)
                                                                 aaCAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGGACGGGGGTGTTTATGGGGTCTGGCAAAACGATTAGCCTTTCCGGGCCGTCGATCATTTTCGTTTTtattatt                                                                            >  2:2043207/1‑139 (MQ=255)
                                                                   cAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCAt                                                                          <  1:1106820/140‑1 (MQ=255)
                                                                     gTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGGTGTTTTTGGGGTCTGGCAAAAAGATTAGCCTTGCCGGGCCGGCGGTCATTTTCGTTTTTATGATCAttt                                                                        >  2:655825/1‑139 (MQ=255)
                                                                     gTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGGTGTTTATGGGGGCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTg                                                                        >  1:316052/1‑140 (MQ=255)
                                                                      tCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGGTGTTTTTGGGGTCTGGCAAAACGATTAGCCTTTCCGGGCCGTCGGTCATTTTTGTTTTTATTATCATTgg                                                                       >  2:1121514/1‑140 (MQ=255)
                                                                        gCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTACCATTTGTAAGGGGGTGTTTTTGGGGTCTGGCAAAACCCATAGGCTTGACGGGCCCACGGTCATTTTCGTTTTTATTATCATTGGtt                                                                     >  2:1492687/1‑140 (MQ=255)
                                                                             ggCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATg                                                                <  1:744831/140‑1 (MQ=255)
                                                                              gcgcAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGGTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGc                                                               >  2:1989519/1‑140 (MQ=255)
                                                                                    tctcACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGGTGTTTTTGGGGGCTGGCAAAACGATTAGCCTTTCCGGGGCGTCGGTCATTTTTGTTTATATGATCATTGGTTTTTTGCTCtttt                                                         >  2:1875002/1‑140 (MQ=255)
                                                                                      tcACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTc                                                       <  1:782650/140‑1 (MQ=255)
                                                                                      tcACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTc                                                       <  1:133499/140‑1 (MQ=255)
                                                                                       cacaAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCg                                                      <  1:1121514/140‑1 (MQ=255)
                                                                                           aaCCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGAt                                                  <  1:330108/140‑1 (MQ=255)
                                                                                              cGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATGCg                                               <  1:1711504/140‑1 (MQ=255)
                                                                                              cGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATGCg                                               <  1:77800/140‑1 (MQ=255)
                                                                                                aCATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATGCggg                                             <  2:1220017/140‑1 (MQ=255)
                                                                                                 cATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATGCGGGc                                            <  1:1840588/140‑1 (MQ=255)
                                                                                                   tatTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTGTTTATGGTGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATGCGGGCaa                                          <  2:1173089/140‑1 (MQ=255)
                                                                                                    atTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATGCGGGCAAt                                         <  1:843039/140‑1 (MQ=255)
                                                                                                          cTTATTGCCATTGGCGGTGCCCTTGGTACGGGGGTGTTTTTGGGGTCTGGCAAAAAGAATAGCCTTGCCGGGCCGTCTATCATTTTCGTTTATATGATAATTGGTTTTTTGCTCTTTTTCTTGATTCGGGCCATgggggg                                   >  2:175106/1‑139 (MQ=255)
                                                                                                              ttGCCATTGGCGGTGCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATGCGGGCAATGGGGGAATtg                               <  1:175106/140‑1 (MQ=255)
                                                                                                              ttGCCATTGGCGGTGCCATTGGGACGGGGGTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGGCGGCGATCATTTTTGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATGCGGGCAATGGGGGGATtg                               >  2:63835/1‑140 (MQ=255)
                                                                                                                   aTTGGCGGTGCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATGCGGGCAATGGGGGAATTgctgct                          <  2:2091952/140‑1 (MQ=255)
                                                                                                                            gCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATGCGGGCAATGGGGGAATtgct                             <  1:275356/128‑1 (MQ=255)
                                                                                                                            gCCATTGGTACGGGGGTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATGCGGGCAATGGGGGAATtgct                             >  2:275356/1‑128 (MQ=255)
                                                                                                                                 tGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATGCGGGCAATGGGGGAATTgctgct                          >  1:582119/1‑126 (MQ=255)
                                                                                                                                 tGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATGCGGGCAATGGGGGAATTgctgct                          <  2:582119/126‑1 (MQ=255)
                                                                                                                                  ggTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATGCGGGCAATGGGGGAATtgct                             <  1:1628392/122‑1 (MQ=255)
                                                                                                                                  ggTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATGCGGGCAATGGGGGAATTGCTGCTTTCGAATCTGGAATa           >  1:1910254/1‑140 (MQ=255)
                                                                                                                                    tACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATGCGGGCAATGGGGGAATTGCTGCTTTCGAATCTGGAATACa         <  2:316052/140‑1 (MQ=255)
                                                                                                                                     aCGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCAATTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATGCGGGCAATGGGGGAATTGCTGCTTTCGAATCTGGAATACaa        >  1:1847616/1‑140 (MQ=255)
                                                                                                                                       ggggTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATGCGGGCAATGGGGGAATTGCTGCTTTCGAATCTGGAATACAAAt      <  1:1989519/140‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                           
GGTACAGGAAGAAAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATGCGGGCAATGGGGGAATTGCTGCTTTCGAATCTGGAATACAAATCTTT  >  NC_000913/4429845‑4430123

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑