Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,401,459	0	T	C	22.0%	115.1 / 12.0	59	D625G (GAT→GGT)	csrD	regulator of CsrB and CsrC decay
Reads supporting (aligned to +/- strand):  ref base T (35/11);  new base C (0/13);  total (35/24)
Fisher's exact test for biased strand distribution p-value = 6.17e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

GCGAGCAGACAAGCACACTCCCATGAGACGCAGCGCGCATTATTCTACGTGAAAACGGATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGAACCAGCAGCTGGTTCTCCGTT  >  NC_000913/3401321‑3401596                                                                                                                                           |                                                                                                                                          gCGAGCAGACAAGCACACTCCCATGAGACGCAGCGCGCATTATTCTACGTGAAAACGGATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAATc                                                                                                                                          >  1:201779/1‑140 (MQ=255)        gACAAGCACACTCCCATGAGACGCAGCGCGCATTATTCTACGTGAAAACGGATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAATCCCCTTGc                                                                                                                                   <  1:243568/140‑1 (MQ=255)        gACAAGCACACTCCCATGAGACGCAGCGCGCATTATTCTACGTGAAAACGGATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCAAAAAAATCCCCTTGc                                                                                                                                   <  1:649905/140‑1 (MQ=255)          cAAGCACACTCCCATGAGACGCAGCGCGCATTATTCTACGTGAAAACGGATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAATCCCCTTGccc                                                                                                                                 <  2:447540/140‑1 (MQ=255)          cAAGCACACTCCCATGAGACGCAGCGCGCATTATTCTACGTGAAAACGGATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAATCCCCTTGccc                                                                                                                                 <  2:40174/140‑1 (MQ=255)          cAAGCACACTCCCATGAGACGCAGCGCGCATTATTCTACGTGAAAACGGATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAACCCCCTTGccc                                                                                                                                 <  2:1353437/140‑1 (MQ=255)          cAAGCACACCCCCATGACACGCAGCCCGCATTATTCTACGTGAAAACGGTTTAACCGGCAGGTAAACCCGAGTATCTTTGTGAATATTTTTCCCCGTTGGTATCAAGTGGCTGGGAGGACGCGAAAAAACCCCCTTGccc                                                                                                                                 <  2:860462/140‑1 (MQ=255)           aaGCACACTCCCATGAGACGCAGCGCGCATTATTCTACGTGAAAACGGATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAATCCCCTTGCCCg                                                                                                                                >  1:674269/1‑140 (MQ=255)            aGCACACTCCCATGAGACGCAGCGCGCATTATTCTACGTGAAAACGGATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAATCCCCTTGCCCGc                                                                                                                               <  2:201779/140‑1 (MQ=255)                   tCCCATGAGACGCAGCGCGCATTATTCTACGTGAAAACGGATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAATCCCCTTGCCCGCCTGTAac                                                                                                                        >  2:384027/1‑140 (MQ=255)                    cccATGAGACGCAGCGCGCATTATTCTACGTGAAAACGGATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAATCCCCTTGCCCGCCTGTAaca                                                                                                                       <  1:27226/140‑1 (MQ=255)                     ccATGAGACGCAGCGCGCATTATTCTACGTGAAAACGGATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAACCCCCTTGCCCGCCTGTAacac                                                                                                                      <  2:1316894/140‑1 (MQ=255)                       aTGAGACGCAGCGCGCATTATTCTACGTGAAAACGGATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACcg                                                                                                                    >  2:1238515/1‑140 (MQ=255)                       aTGAGACGCAGCGCGCATTATTCTACGTGAAAACGGATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAATCCCCTAGCCCGCCTGTAACACcg                                                                                                                    >  1:1204257/1‑140 (MQ=255)                        tGAGACGCAGCGCGCATTATTCTACGTGAAAACGGATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACcgc                                                                                                                   >  1:664555/1‑140 (MQ=255)                         gagcCGCAGCGCGCTTTATTCTACGTAAAAACGGATTAACCGGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCAGGTTAGTATCAAGTGGCTGGGAGGACGCGAAAAAACCCCCTTGCCCGCCTGTAACACcgcg                                                                                                                  <  2:1242316/140‑1 (MQ=255)                             cGCAGCGCGCATTATTCTACGTGAAAACGGATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGa                                                                                                              >  1:486948/1‑140 (MQ=255)                               cAGCGCGCATTATTCTACGTGAAAACGGATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAAt                                                                                                            >  2:1021546/1‑140 (MQ=255)                                  cgcgcATTATTCTACGTGAAAACGGATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAg                                                                                                         >  2:4324/1‑140 (MQ=255)                                    cgcATTATTCTACGTGAAAACGGATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAggg                                                                                                       >  2:75476/1‑140 (MQ=255)                                     gcATTATTCTACGTGAAAACGGATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCAAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGt                                                                                                      <  1:1052667/140‑1 (MQ=255)                                                 gTGAAAACGGATTAAACGGCAGGTTAAACCGAGTATCTTTGTGTATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACATCGCGCTGAATCAGGGTCTGCCACTCGCt                                                                                          >  1:1148507/1‑140 (MQ=255)                                                   gAAAACGGATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTc                                                                                        >  1:992838/1‑140 (MQ=255)                                                     aaaCGGATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTcgc                                                                                      >  1:10507/1‑140 (MQ=255)                                                                 ggggaGGTGAAGCCGAGTGTCTTTGTGAATATTTTTCCATGTGAGTTTCACGGGGCTGTGAGGACGGAAAAAATTCCCCTGCCCCGCCTGTAACCCCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCg                                                                          <  2:486948/136‑1 (MQ=255)                                                                    cAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTg                                                                       >  2:723387/1‑140 (MQ=255)                                                                       gTTAAACCGATTATTTTTGTGAATTTTTTTTCACGTTATTATCAAGTGGCTGTGAGGACGCAAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCg                                                                    <  2:1111799/140‑1 (MQ=255)                                                                         tAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAACCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTa                                                                  <  1:880436/140‑1 (MQ=255)                                                                                aGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTgg                                                           >  2:912521/1‑140 (MQ=255)                                                                                 gTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTggg                                                          >  2:262651/1‑140 (MQ=255)                                                                                 gTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTggg                                                          >  1:953549/1‑140 (MQ=255)                                                                                 gTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTggg                                                          >  1:771094/1‑140 (MQ=255)                                                                                 gTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCGGTGAGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACTCACGCCGGTGGCGTAAACCTggg                                                          >  2:613476/1‑140 (MQ=255)                                                                                     cTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCt                                                      <  1:75476/140‑1 (MQ=255)                                                                                          tgAATTTTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAACCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTcc                                                 <  1:1362839/140‑1 (MQ=255)                                                                                          tgAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTcc                                                 >  1:724947/1‑140 (MQ=255)                                                                                          tgAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAACCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTcc                                                 <  2:724947/140‑1 (MQ=255)                                                                                          catattttttttCAGGTTAGTATCAAGTGGTTGTGGGGACGCAAAAAAACCCCCTGCCCCGCCTGTAACACCGCGCTGAATCAGGGTCTTCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTcc                                                 <  2:1351127/134‑1 (MQ=255)                                                                                            aaTTTTTTTTCACGTTAGTATCAAGTGGCTGGGAGGCCGCAAAAAACCCCCCTGGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCg                                               <  2:461736/140‑1 (MQ=255)                                                                                               atTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAACCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAg                                            <  2:10507/140‑1 (MQ=255)                                                                                                ttttttCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGc                                           >  2:132187/1‑140 (MQ=255)                                                                                                    ttCCCGTTAGTATAAAGGGGCTGTGAGGACGCAAAAAAACCCCCTGGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGc                                       <  2:812815/140‑1 (MQ=255)                                                                                                        ctttAGTATCAAGTGGCTGTGAGGACGCGAAAAAACCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTcc                                   <  1:329860/138‑1 (MQ=255)                                                                                                        cGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTTCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCtctg                                   >  1:1351127/1‑137 (MQ=255)                                                                                                          ttAGTATCAAGTGGCTGTGAGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTccac                                 >  1:232931/1‑140 (MQ=255)                                                                                                                   aGTGGCTGTGAGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTg                        >  1:554281/1‑140 (MQ=255)                                                                                                                   aGTGGCTGTGAGGACGCGAAAAAATCCCCTTGCCAGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTg                        >  1:1149664/1‑140 (MQ=255)                                                                                                                    gTGGCTGTGAGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGa                       <  1:262651/140‑1 (MQ=255)                                                                                                                     tGGCTGTGAGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGaa                      >  1:701746/1‑140 (MQ=255)                                                                                                                     tGGCTGTGAGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCCGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGaa                      >  1:481931/1‑140 (MQ=255)                                                                                                                       gCTGTGAGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGAAcc                    >  1:386378/1‑140 (MQ=255)                                                                                                                       gCTGTGAGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGAAcc                    >  1:494167/1‑140 (MQ=255)                                                                                                                       gCTGTGAGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGAAcc                    >  1:885440/1‑140 (MQ=255)                                                                                                                        cTGTGAGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGAACca                   >  1:365857/1‑140 (MQ=255)                                                                                                                        cTGTGAGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGAACca                   >  2:1378228/1‑140 (MQ=255)                                                                                                                        cTGTGAGGACGCAAAAAAACCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGAACca                   <  2:774990/140‑1 (MQ=255)                                                                                                                             aGGACGCAAAAAACCCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGAACCagcagc              <  2:365857/140‑1 (MQ=255)                                                                                                                               gACGCGAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGAACCAGCAGCTg            >  2:669872/1‑140 (MQ=255)                                                                                                                                aCGCGAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGAACCAGCAGCTgg           >  2:237576/1‑140 (MQ=255)                                                                                                                                 cgcgAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGAACCAGCAGCTGGt          >  1:843191/1‑140 (MQ=255)                                                                                                                                  gcgAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGAACCAGCAGCTGGtt         >  1:436129/1‑140 (MQ=255)                                                                                                                                         aaTCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGAACCAGCAGCTGGTTCTCCGtt  <  1:613476/140‑1 (MQ=255)                                                                                                                                         aaTCCCCTTGCCAGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGAACCAGCAGCTGGTTCTCCGtt  <  2:1149664/140‑1 (MQ=255)                                                                                                                                           |                                                                                                                                          GCGAGCAGACAAGCACACTCCCATGAGACGCAGCGCGCATTATTCTACGTGAAAACGGATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGAACCAGCAGCTGGTTCTCCGTT  >  NC_000913/3401321‑3401596

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑