Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,111,7470GT24.3% 105.1 / 12.7 37N156K (AAC→AAA) yiiQDUF1454 domain‑containing protein YiiQ
Reads supporting (aligned to +/- strand):  ref base G (27/1);  new base T (0/9);  total (27/10)
Fisher's exact test for biased strand distribution p-value = 8.04e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

CTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCGCCGTTGT‑CCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAGCTTTCGCTTTCGCGGCTTTTTGCTCTGGCCCCTGGATGG  >  NC_000913/4111629‑4111886
                                                                                                                       |                                                                                                                                           
ctacAAGCGATTAAGATACCCCCACCTTAATCGTTCCAACAGCGAAGGCCACCCCCTTTCCGCCGTTGT‑CCGCGACAACATAACGCAGTCCCCCTTCTGTCTGGGTTTAATAACTTTTTTTTTCCCCCGCCGTTAGTAGc                                                                                                                        <  2:510078/137‑1 (MQ=255)
   cAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCGCCGTTGT‑CCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGAc                                                                                                                     >  1:80209/1‑140 (MQ=255)
        gATTCAGATAGCGCCAGTTTAATGGGTAAAACAGCGAAGGTCACCCCTTTTTCGCCGTTGC‑CCGCGAAAACATACCGCAGTCCCCCTTCTGTCTGGGTTTAATACTTTTTTTTTTCCCCCGCCGTTAGTAGCGACTGCAg                                                                                                                <  2:1126479/140‑1 (MQ=255)
        gATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCGCCGTTGT‑CCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAg                                                                                                                >  2:473064/1‑140 (MQ=255)
        gATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCGCCGTTGT‑CCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAg                                                                                                                >  1:384589/1‑140 (MQ=255)
          ttCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCGCCGTTGT‑CCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGtt                                                                                                              >  2:819751/1‑140 (MQ=255)
               acaGCCCCAGTTAAATGGGTACAACAGCAAGGGTCACCCCCTTTCCGCCGTTGC‑CCGCAAAAAAATAACGCGGTCCCCCTTCTGTCTCGGTGTAATAATTTTTTTTTTCCCCCGCCGTTAGTAGCGACTGCAGTTTTTTc                                                                                                         <  2:541155/138‑1 (MQ=255)
                    gcCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCGCCGTTGT‑CCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATGACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCt                                                                                                    >  2:310247/1‑140 (MQ=255)
                    gcCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCGCCGTTGT‑CCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCt                                                                                                    >  2:809976/1‑140 (MQ=255)
                    gcCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCGCCGTTGT‑CCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCt                                                                                                    >  1:325162/1‑140 (MQ=255)
                     ccAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCGCCGTTGT‑CCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCtt                                                                                                   >  1:6902/1‑140 (MQ=255)
                        gCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCGCCGTTGT‑CCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTtgt                                                                                                >  1:1241840/1‑140 (MQ=255)
                         cTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCGCCGTTGT‑CCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTtgtg                                                                                               >  1:1101920/1‑140 (MQ=255)
                             aTCGGTTCAACAGCGAAGGTCAGCCCCTTTTCGCCGTTGT‑CCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGtttt                                                                                           >  2:267248/1‑140 (MQ=255)
                                gtttCAACAGCGAGGGCCACCCCCTTTCCGCCTTTGC‑CCGCAAAAACAAAACGCAGTGCCCCTTTTGTCTCGGTGTAATACCGTTTTTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGt                                                                                        <  2:111191/138‑1 (MQ=255)
                                ggTTCAACAGGGAAGGTCACCCCTTTTCCGCTGTTCC‑CCGCAAAAACATACCGCAGTCCCCCTTCTGTCTGGGTGTAATAATTTTTTTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGt                                                                                        <  2:1241840/140‑1 (MQ=255)
                                  ttCAACAGCGAAGGTCAGCCCCTTTTCGCCGTTGT‑CCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCa                                                                                      >  1:89803/1‑140 (MQ=255)
                                               gtcccccccTTTTCCGCGTTTCCCCGCAACAAAATACCGCGGTCCCCCTTCTGTCGGGGTGTAATATTTTTTTTTTTCCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGa                                                                          <  2:350929/135‑1 (MQ=255)
                                                         tttCGCCGTTGT‑CCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATc                                                               >  2:1222856/1‑140 (MQ=255)
                                                             gCCGTTGT‑CCGCAACAACATAACGCGGTCCCCCTTTTGTCTGGGTGTAATATTTTTTTTTTTCCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCg                                                           <  2:384589/140‑1 (MQ=255)
                                                               cGTTGC‑CCGCAAAAACATAACGCGGTGCACTTTCTGTCTGGGTTTAATAATTTTTTTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCt                                                         <  2:1152069/140‑1 (MQ=255)
                                                                  tGT‑CCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGcc                                                      >  2:754475/1‑140 (MQ=255)
                                                                             aacaTAACGCAGTGCCCCTTCTGTCTCGGTGTAATAACTTTTTTTTTCCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCt                                            <  1:717777/140‑1 (MQ=255)
                                                                                         tGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAgctttcgctt                                >  2:103547/1‑140 (MQ=255)
                                                                                         tGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAgctttcgctt                                >  1:261740/1‑140 (MQ=255)
                                                                                         tGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAgctttcgctt                                >  2:954556/1‑140 (MQ=255)
                                                                                         tGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAgctttcgctt                                >  1:1321340/1‑140 (MQ=255)
                                                                                         tGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAgctttcgctt                                >  2:1319320/1‑140 (MQ=255)
                                                                                          gCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAgctttcgcttt                               >  1:1173474/1‑140 (MQ=255)
                                                                                           cACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAgctttcgctttc                              >  2:292662/1‑140 (MQ=255)
                                                                                            cctttCTGTCTCGGTGTAATATTTTTTTTTTTCCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAGctttcgctttcg                             <  2:1101920/137‑1 (MQ=255)
                                                                                                   gTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAGCTTTCGCTTTCGCGGCttt                      >  1:963757/1‑140 (MQ=255)
                                                                                                   gTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAGCTTTCGCTTTCGCGGCttt                      >  2:47499/1‑140 (MQ=255)
                                                                                                   gTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAGCTTTCGCTTTCGCGGCttt                      >  2:495345/1‑140 (MQ=255)
                                                                                                   gTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAGCTTTCGCTTTCGCGGCttt                      >  2:673692/1‑140 (MQ=255)
                                                                                                        ggTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAGCTTTCGCTTTCGCGGCTTTTTGct                 >  2:72923/1‑140 (MQ=255)
                                                                                                         gtgtAATATTTTTTTTTTTCCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAGCTTTCGCTTTCGCGGCTTTTTGctc                <  1:473064/140‑1 (MQ=255)
                                                                                                          tgtAATAGGGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAGCTTTCGCTTTCGCGGCTTTTTGctct               <  1:47499/140‑1 (MQ=255)
                                                                                                          tgtAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAGCTTTCGCTTTCGCGGCTTTTTGctct               >  1:544580/1‑140 (MQ=255)
                                                                                                            taataaCGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAGCTTTCGCTTTcgcg                           <  1:145024/126‑1 (MQ=255)
                                                                                                            taataaCGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAGCTTTCGCTTTcgcg                           >  2:145024/1‑126 (MQ=255)
                                                                                                                  ttttttttttCCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAGCTTTCGCTTTCGCGGCTTTTTGCTCTGGCCCCTg       <  1:819751/138‑1 (MQ=255)
                                                                                                                    tttgtttTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAGCTTTCGCTTTCGCGGCTTTTTGCTCTGGCCCCTGGa     >  1:240979/1‑140 (MQ=255)
                                                                                                                    tttgtttTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAGCTTTCGCTTTCGCGGCTTTTTGCTCTGGCCCCTGGa     >  1:229191/1‑140 (MQ=255)
                                                                                                                      tgtttTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAGCTTTCGCTTTCGCGGCTTTTTGCTCTGGCCCCTGGATg   >  2:945265/1‑140 (MQ=255)
                                                                                                                      tgtttTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAGCTTTCGCTTTCGCGGCTTTTTGCTCTGGCCCCTGGATg   >  2:1114746/1‑140 (MQ=255)
                                                                                                                       gtttTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAGCTTTCGCTTTCGCGGCTTTTTGCTCTGGCCCCTGGATgg  >  1:871466/1‑140 (MQ=255)
                                                                                                                       |                                                                                                                                           
CTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCGCCGTTGT‑CCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAGCTTTCGCTTTCGCGGCTTTTTGCTCTGGCCCCTGGATGG  >  NC_000913/4111629‑4111886

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑