Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	505,107	G→T	G65V (GGT→GTT)	ushA →	5'‑nucleotidase/UDP‑sugar hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	505,107	0	G	T	100.0%	327.8 / NA	93	G65V (GGT→GTT)	ushA	5'‑nucleotidase/UDP‑sugar hydrolase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (45/48);  total (45/48)

AGTGAAACTGCTCTGGCGTATGAGCAGGATAAAACCTACAAAATTACAGTTCTGCATACCAATGATCATCATGGGCATTTTTGGCGCAATGAATATGGCGAATATGGTCTGGCGGCGCAAAAAACGCTGGTGGATGGTATCCGCAAAGAGGTTGCGGCTGAAGGCGGTAGCGTGCTGCTACTTTCCGGTGGCGACATTAACACTGGCGTGCCCGAGTCTGACTTACAGGATGCCGAACCTGATTTTCGCGGTATGAATCTGGTGGGCTATGA  >  NC_000913/504971‑505242                                                                                                                                         |                                                                                                                                        aGTGAAACTGCTCTGGCGTATGAGCAGGATAAAACCTACAAAATTACAGTTCTGCATACCAATGATCATCATGGGCATTTTTGGCGCAATGAATATGGCGAATATGGTCTGGCGGCGCAAAAAACGCTGGTGGATGTTAt                                                                                                                                      <  2:1029112/140‑1 (MQ=255) aGTGAAACTGCTCTGGCGTATGAGCAGGATAAAACCTACAAAATTACAGTTCTGCATACCAATGATCATCATGGGCATTTTTGGCGCAATGAATATGGCGAATATGGTCTGGCGGCGCAAAAAACGCTGGTGGATGTTAt                                                                                                                                      <  2:269159/140‑1 (MQ=255) aGTGAAACTGCTCTGGCGTATGAGCAGGATAAAACCTACAAAATTACAGTTCTGCATACCAATGATCATCATGGGCATTTTTGGCGCAATGAATATGGCGAATATGGTCTGGCGGCGCAAAAAACGCTGGTGGATGTTAt                                                                                                                                      >  1:1383901/1‑140 (MQ=255)   tGAAACTGCTCTGGCGTATGAGCAGGATAAAACCTACAAAATTACAGTTCTGCATACCAATGATCATCATGGGCATTTTTGGCGCAATGAATATGGCGAATATGGTCTGGCGGCGCAAAAAACGCTGGTGGATGTTATcc                                                                                                                                    >  2:1228369/1‑140 (MQ=255)   tGAAACTGCTCTGGCGTATGAGCAGGATAAAACCTACAAAATTACAGTTCTGCATACCAATGATCATCATGGGCATTTTTGGCGCAATGAATATGGCGAATATGGTCTGGCGGCGCAAAAAACGCTGGTGGATGTTATcc                                                                                                                                    >  1:501111/1‑140 (MQ=255)      aaCTGCTCTGGCGTATGAGCAGGATAAAACCTACAAAATTACAGTTCTGCATACCAATGATCATCATGGGCATTTTTGGCGCAATGAATATGGCGAATATGGTCTGGCGGCGCAAAAAACGCTGGTGGATGTTATCCGCa                                                                                                                                 <  1:129643/140‑1 (MQ=255)        cTGCTCTGGCGTATGAGCAGGATAAAACCTACAAAATTACAGTCCTGCATACCAATGATCATCATGGGCATTTTTGGCGCAATGAATATGGCGAATATGGTCTGGCGGCGCAAAAAACGCTGGTGGATGTTATCCGCaaa                                                                                                                               >  2:473108/1‑140 (MQ=255)             ctGGCGTATGAGCAGGATAAAACCTACAAAATTACAGTTCTGCATACCAATGATCATCATGGGCATTTTTGGCGCAATGAATATGGCGAATATGGTCTGGCGGCGCAAAAAACGCTGGTGGATGTTATCCGCAAAGAGGt                                                                                                                          >  1:41060/1‑140 (MQ=255)               ggCGTATGAGCAGGATAAAACCTACAAAATTACAGTTCTGCATACCAATGATCATCATGGGCATTTTTGGCGCAATGAATATGGCGAATATGGTCTGGCGGCGCAAAAAACGCTGGTGGATGTTATCCGCAAAGAGGTTg                                                                                                                        <  1:1354246/140‑1 (MQ=255)                 cGTATGAGCAGGATAAAACCTACAAAATTACAGTTCTGCATACCAATGATCATCATGGGCATTTTTGGCGCAATGAGTATGGCGAATATGGTCTGGCGGCGCAAAAAACGCTGGTGGATGTTATCCGCAAAGAGGTTGCg                                                                                                                      >  2:431441/1‑140 (MQ=255)                  gTATGAGCAGGATAAAACCTACAAAATTACAGTTCTGCATACCAATGATCATCATGGGCATTTTTGACGCAATGAATATGGCGAATATGGTCTGGCGGCGCAAAAAACGCTGGTGGATGTTATCCGCAAAGAGGTTGCgg                                                                                                                     <  2:1021776/140‑1 (MQ=255)                     tGAGCAGGATAAAACCTACAAAATTACAGTTCTGCATACCAATGATCATCATGGGCATTTTTGGCGCAATGAATATGGCGAATATGGTCTGGCGGCGCAAAAAACGCTGGTGGATGTTATCCGCAAAGAGGTTGCGGCTg                                                                                                                  >  1:1053786/1‑140 (MQ=255)                      gAGCAGGATAAAACCTACAAAATTACAGTTCTGCATACCAATGATCATCATGGGCATTTTTGGCGCAATGAATATGGCGAATATGGTCTGGCGGCGCAAAAAACGCTGGTGGATGTTATCCGCAAAGAGGTTGCGGCTGa                                                                                                                 <  2:87418/140‑1 (MQ=255)                        gCAGGATAAAACCTACAAAATTACAGTTCTGCATACCAATGATCATCATGGGCATTTTTGGCGCAATGAATATGGCGAATATGGTCTGGCGGCGCAAAAAACGCTGGTGGATGTTATCCGCAAAGAGGTTGCGGCTGAAg                                                                                                               >  1:972648/1‑140 (MQ=255)                         cAGGATAAAACCTACAAAATTACAGTTCTGCATACCAATGATCATCATGGGCATTTTTGGCGCAATGAATATGGCGAATATGGTCTGGCGGCGCAAAAAACGCTGGTGGATGTTATCCGCAAAGAGGTTGCGGCTGAAgg                                                                                                              <  2:692459/140‑1 (MQ=255)                          aGGATAAAACCTACAAAATTACAGTTCTGCATACCAATGATCATCATGGGCATTTTTGGCGCAATGAATATGGCGAATATGGTCTGGCGGCGCAAAAAACGCTGGTGGATGTTATCCGCAAAGAGGTTGTGGCt                                                                                                                   >  1:1323971/1‑134 (MQ=255)                          aGGATAAAACCTACAAAATTACAGTTCTGCATACCAATGATCATCATGGGCATTTTTGGCGCAATGAATATGGCGAATATGGTCTGGCGGCGCAAAAAACGCTGGTGGATGTTATCCGCAAAGAGGTTGTGGCt                                                                                                                   <  2:1323971/134‑1 (MQ=255)                          aGGATAAAACCTACAAAATTACAGTTCTGCATACCAATGATCATCATGGGCATTTTTGGCGCAATGAATATGGCGAATATGGTCTGGCGGCGCAAAAAACGCTGGTGGATGTTATCCGCAAAGAGGTTGCGGCTGAAGGc                                                                                                             >  2:1342714/1‑140 (MQ=255)                             aTAAAACCTACAAAATTACAGTTCTGCATACCAATGATCATCATGGGCATTTTTGGCGCAATGAATATGGCGAATATGGTCTGGCGGCGCAAAAAACGCTGGTGGATGTTATCCGCAAAGAGGTTGCGGCTGAAGGCGGt                                                                                                          <  2:1260371/140‑1 (MQ=255)                               aaaaCCTACAAAATTACAGTTCTGCATACCAATGATCATCATGGGCATTTTTGGCGCAATGAATATGGCGAATATGGTCTGGCGGCGCAAAAAACGCTGGTGGATGTTATCCGCAAAGAGGTTGCGGCTGAAGGCGGTAg                                                                                                        <  1:128575/140‑1 (MQ=255)                               aaaaCCTACAAAATTACAGTTCTGCATACCAATGATCATCATGGGCATTTTTGGCGCAATGAATATGGCGAATATGGTCTGGCGGCGCAAAAAACGCTGGTGGATGTTATCCGCAAAGAGGTTGCGGCTGAAGGCGGTAg                                                                                                        <  2:926073/140‑1 (MQ=255)                               aaaaCCTACAAAATTACAGTTCTGCATACCAATGATCATCATGGGCATTTTTGGCGCAATGAATATGGCGAATATGGTCTGGCGGCGCAAAAAACGCTGGTGGATGTTATCCGCAAAGAGGTTGCGGCTGAAGGCGGTAg                                                                                                        <  2:1224483/140‑1 (MQ=255)                                 aaCCTACAAAATTACAGTTCTGCATACCAATGATCATCATGGGCATTTTTGGCGCAATGAATATGGCGAATATGGTCTGGCGGCGCAAAAAACGCTGGTGGATGTTATCCGCAAAGAGGTTGCGGCTGAAGGCGGTAGCg                                                                                                      >  1:542917/1‑140 (MQ=255)                                    cTACAAAATTACAGTTCTGCATACCAATGATCATCATGGGCATTTTTGGCGCAATGAATATGGCGAATATGGTCTGGCGGCGCAAAAAACGCTGGTGGATGTTATCCGCAAAGAGGTTGCGGCTGAAGGCGGTAGCGtgc                                                                                                   <  1:211190/140‑1 (MQ=255)                                      aCAAAATTACAGTTCTGCATACCAATGATCATCATGGGCATTTTTGGCGCAATGAATATGGCGAATATGGTCTGGCGGCGCAAAAAACGCTGGTGGATGTTATCCGCAAAGAGGTTGCGGCTGAAGGCGGTAGCGtgctg                                                                                                 >  2:1338723/1‑140 (MQ=255)                                      aCAAAATTACAGTTCTGCATACCAATGATCATCATGGGCATTTTTGGCGCAATGAATATGGCGAATATGGTCTGGCGGCGCAAAAAACGCTGGTGGATGTTATCCGCAAAGAGGTTGCGGCTGAAGGCGGTAGCGtgctg                                                                                                 >  1:76746/1‑140 (MQ=255)                                      aCAAAATTACAGTTCTGCATACCAATGATCATCATGGGCATTTTTGGCGCAATGAATATGGCGAATATGGTCTGGCGGCGCAAAAAACGCTGGTGGATGTTATCCGCAAAGAGGTTGCGGCTGAAGGCGGTAGCGtgctg                                                                                                 >  2:874024/1‑140 (MQ=255)                                      aCAAAATTACAGTTCTGCATACCAATGATCATCATGGGCATTTTTGGCGCAATGAATATGGCGAATATGGTCTGGCGGCGCAAAAAACGCTGGTGGATGTTATCCGCAAAGAGGTTGCGGCTGAAGGCGGTAGCGtgctg                                                                                                 >  1:1254217/1‑140 (MQ=255)                                      aCAAAATTACAGTTCTGCATACCAATGATCATCATGGGCATTTTTGGCGCAATGAATATGGCGAATATGGTCTGGCGGCGCAAAAAACGCTGGTGGATGTTATCCGCAAAGAGGTTGCGGCTGAAGGCGGTAGCGtgctg                                                                                                 >  1:1262160/1‑140 (MQ=255)                                      aCAAAATTACAGTTCTGCATACCAATGATCATCATGGGCATTTTTGGCGCAATGAATATGGCGAATATGGTCTGGCGGCGCAAAAAACGCTGGTGGATGTTATCCGCAAAGAGGTTGCGGCTGAAGGCGGTAGCGtgctg                                                                                                 >  2:654040/1‑140 (MQ=255)                                             tACAGTTCTGCATACCAATGATCATCATGGGCATTTTTGGCGCAATGAATATGGCGAATATGGTCTGGCGGCGCAAAAAACGCTGGTGGATGTTATCCGCAAAGAGGTTGCGGCTGAAGGCGGTAGCGTGCTGCTACttt                                                                                          >  1:400430/1‑140 (MQ=255)                                              aCAGTTCTGCATACCAATGATCATCATGGGCATTTTTGGCGCAATGAATATGGCGAATATGGTCTGGCGGCGCAAAAAACGCTGGTGGATGTTATCCGCAAAGAGGTTGCGGCTGAAGGCGGTAGCGTGCTGCTACTTTc                                                                                         >  2:516784/1‑140 (MQ=255)                                                   tCTGCATACCAATGATCATCATGGGCATTTTTGGCGCAATGAATATGGCGAATATGGTCTGGCGGCGCAAAAAACGCTGGTGGATGTTATCCGCAAAGAGGTTGCGGCTGAAGGCGGTAGCGTGCTGCTACTTTCCGGTg                                                                                    <  2:41060/140‑1 (MQ=255)                                                   tCTGCATACCAATGATCATCATGGGCATTTTTGGCGCAATGAATATGGCGAATATGGTCTGGCGGCGCAAAAAACGCTGGTGGATGTTATCCGCAAAGAGGTTGCGGCTGAAGGCGGTAGCGTGCTGCTACTTTCCGGTg                                                                                    <  2:972648/140‑1 (MQ=255)                                                   tCTGCATACCAATGATCATCATGGGCATTTTTGGCGCAATGAATATGGCGAATATGGTCTGGCGGCGCAAAAAACGCTGGTGGATGTTATCCGCAAAGAGGTTGCGGCTGAAGGCGGTAGCGTGCTGCTACTTTCCGGTg                                                                                    <  1:516784/140‑1 (MQ=255)                                                    cTGCATACCAATGATCATCATGGGCATTTTTGGCGCAATGAATATGGCGAATATGGTCTGGCGGCGCAAAAAACGCTGGTGGATGTTATCCGCAAAGAGGTTGCGGCTGAAGGCGGTAGCGTGCTGCTACTTTCCGGTgg                                                                                   >  2:551936/1‑140 (MQ=255)                                                     tGCATACCAATGATCATCATGGGCATTTTTGGCGCAATGAATATGGCGAATATGGTCTGGCGGCGCAAAAAACGCTGGTGGATGTTATCCGCAAAGAGGTTGCGGCTGAAGGCGGTAGCGTGCTGCTACTTTCCGGTGGc                                                                                  <  1:874024/140‑1 (MQ=255)                                                       cATACCAATGATCATCATGGGCATTTTTGGCGCAATGAATATGGCGAATATGGTCTGGCGGCGCAAAAAACGCTGGTGGATGTTATCCGCAAAGAGGTTGCGGCTGAAGGCGGTAGCGTGCTGCTACTTTCCGGTGGCGa                                                                                >  2:123093/1‑140 (MQ=255)                                                          aCCAATGATCATCATGGGCATTTTTGGCGCAATGAATATGGCGAATATGGTCTGGCGGCGCAAAAAACGCTGGTGGATGTTATCCGCAAAGAGGTTGCGGCTGAAGGCGGTAGCGTGCTGCTACTTTCCGGTGGCGACAt                                                                             <  2:759675/140‑1 (MQ=255)                                                              aTGATCATCATGGGCATTTTTGGCGCAATGAATATGGCGAATATGGTCTGGCGGCGCAAAAAACGCTGGTGGATGTTATCCGCAAAGAGGTTGCGGCTGAAGGCGGTAGCGTGCTGCTACTTTCCGGTGGCGAc                                                                               <  1:752190/134‑1 (MQ=255)                                                              aTGATCATCATGGGCATTTTTGGCGCAATGAATATGGCGAATATGGTCTGGCGGCGCAAAAAACGCTGGTGGATGTTATCCGCAAAGAGGTTGCGGCTGAAGGCGGTAGCGTGCTGCTACTTTCCGGTGGCGACATTAac                                                                         >  2:1181702/1‑140 (MQ=255)                                                              aTGATCATCATGGGCATTTTTGGCGCAATGAATATGGCGAATATGGTCTGGCGGCGCAAAAAACGCTGGTGGAAGTTATCCGCAAAGAGGTTGCGGCTGAAGGCGGTAGCGTGCTGCTACTTTCCGGTGGCGAc                                                                               >  2:752190/1‑134 (MQ=255)                                                               tGATCATCATGGGCATTTTTGGCGCAATGAATATGGCGAATATGGTCTGGCGGCGCAAAAAACGCTGGTGGATGTTATCCGCAAAGAGGTTGCGGCTGAAGGCGGTAGCGTGCTGCTACTTTCCGGTGGc                                                                                  <  1:1305481/130‑1 (MQ=255)                                                               tGATCATCATGGGCATTTTTGGCGCAATGAATATGGCGAATATGGTCTGGCGGCGCAAAAAACGCTGGTGGATGTTATCCGCAAAGAGGTTGCGGCTGAAGGCGGTAGCGTGCTGCTACTTTCCGGTGGc                                                                                  >  2:1305481/1‑130 (MQ=255)                                                               tGATCATCATGGGCATTTTTGGCGCAATGAATATGGCGAATATGGTCTGGCGGCGCAAAAAACGCTGGTGGATGTTATCCGCAAAGAGGTTGCGGCTGAAGGCGGTAGCGTGCTGCTACTTTCCGGTGGCGACATTAaca                                                                        >  1:461373/1‑140 (MQ=255)                                                                 atcatcATGGGCATTTTTGGCGCAATGAATATGGCGAATATGGTCTGGCGGCGCAAAAAACGCTGGTGGATGTTATCCGCAAAGAGGTTGCGGCTGAAGGCGGTAGCGTGCTGCTACTTTCCGGTGGCGACATTAACACt                                                                      >  2:948617/1‑140 (MQ=255)                                                                 atcatcATGGGCATTTTTGGCGCAATGAATATGGCGAATATGGTCTGGCGACGCAAAAAACGCTGGTGGATGTTATCCGCAAAGAGGTTGCGGCTGAAGGCGGTAGCGTGCTGCTACTTTCCGGTGGCGACATTAACACt                                                                      >  2:278326/1‑140 (MQ=255)                                                                    atcatGGGCATTTTTGGCGCAATGAATATGGCGAATATGGTCTGGCGGCGCAAAAAACGCTGGTGGATGTTATCCGCAAAGAGGTTGCGGCTGAAGGCGGTAGCGTGCTGCTACTTTCCGGTGGCGACATTAACACTGGc                                                                   >  1:470190/1‑140 (MQ=255)                                                                    atcatGGGCATTTTTGGCGCAATGAATATGGCGAATATGGTCTGGCGGCGCAAAAAACGCTGGTGGATGTTATCCGCAAAGAGGTTGCGGCTGAAGGCGGTAGCGTGCTGCTACTTTCCGGTGGCGACATTAACACTGGc                                                                   <  1:460950/140‑1 (MQ=255)                                                                      catGGGCATTTTTGGCGCAATGAATATGGCGAATATGGTCTGGCGGCGCAAAAAACGCTGGTGGATGTTATCCGCAAAGAGGTTGCGGCTGAAGGCGGTAGCGTGCTGCTACTTTCCGGTGGCGACATTAACACTGGCGt                                                                 >  1:884630/1‑140 (MQ=255)                                                                        tGGGCATTTTTGGCGCAATGAATATGGCGAATATGGTCTGGCGGCGCAAAAAACGCTGGTGGATGTTATCCGCAAAGAGGTTGCGGCTGAAGGCGGTAGCGTGCTGCTACTTTCCGGTGGCGACATTAACACTGGCGTGc                                                               <  1:569668/140‑1 (MQ=255)                                                                         gggCATTTTTGGCGCAATGAATATGGCGAATATGGTCTGGCGGCGCAAAAAACGCTGGTGGATGTTATCCGCAAAGAGGTTGCGGCTGAAGGCGGTAGCGTGCTGCTACTTTCCGGTGGCGACATTAACACTGGCGTGcc                                                              <  2:501111/140‑1 (MQ=255)                                                                            cATTTTTGGCGCAATGAATATGGCGAATATGGTCTGGCGGCGCAAAAAACGCTGGTGGATGTTATCCGCAAAGAGGTTGCGGCTGAAGGCGGTAGCGTGCTGCTACTTTCCGGTGGCGACATTAACACTGGCGTGCCCGa                                                           >  2:702442/1‑140 (MQ=255)                                                                            cATTTTTGGCGCAATGAATATGGCGAATATGGTCTGGCGGCGCAAAAAACGCTGGTGGATGTTATCCGCAAAGAGGTTGCGGCTGAAGGCGGTAGCGTGCTGCTACTTTCCGGTGGCGACATTAACACTGGCGTGCCCGa                                                           <  2:470190/140‑1 (MQ=255)                                                                             aTTTTTGGCGCAATGAATATGGCGAATATGGTCTGGCGGCGCAAAAAACGCTGGTGGATGTTATCCGCAAAGAGGTTGCGGCTGAAGGCGGTAGCGTGCTGCTACTTTCCGGTGGCGACATTAACACTGGCGTGCCCGAg                                                          >  1:1281681/1‑140 (MQ=255)                                                                             aTTTTTGGCGCAATGAATATGGCGAATATGGTCTGGCGGCGCAAAAAACGCTGGTGGATGTTATCCGCAAAGAGGTTGCGGCTGAAGGCGGTAGCGTGCTGCTACTTTCCGGTGGCGACATTAACACTGGCGTGCCCGAg                                                          >  2:804465/1‑140 (MQ=255)                                                                              tttttGGCGCAATGAATATGGCGAATATGGTCTGGCGGCGCAAAAAACGCTGGTGGATGTTATCCGCAAAGAGGTTGCGGCTGAAGGCGGTAGCGTGCTGCTACTTTCCGGTGGCGACATTAACACTGGCGTGCCCGAGt                                                         <  1:519614/140‑1 (MQ=255)                                                                              tttttGGCGCAATGAATATGGCGAATATGGTCTGGCGGCGCAAAAAACGCTGGTGGATGTTATCCGCAAAGAGGTTGCGGCTGAAGGCGGTAGCGTGCTGCTACTTTCCGGTGGCGACATTAACACTGGCGTGCCCGAGt                                                         <  2:1383901/140‑1 (MQ=255)                                                                               ttttGGCGCAATGAATATGGCGAATATGGTCTGGCGGCGCAAAAAACGCTGGTGGATGTTATCCGCAAAGAGGTTGCGGCTGAAGGCGGTAGCGTGCTGCTACTTTCCGGTGGCGACATTAACACTGGCGTGCCCGAGTc                                                        <  1:702442/140‑1 (MQ=255)                                                                               ttttGGCGCAATGAATATGGCGAATATGGTCTGGCGGCGCAAAAAACGCTGGTGGATGTTATCCGCAAAGAGGTTGCGGCTGAAGGCGGTAGCGTGCTGCTACTTTCCGGTGGCGACATTAACACTGGCGTGCCCGAGTc                                                        <  1:123093/140‑1 (MQ=255)                                                                               ttttGGCGCAATGAATATGGCGAATATGGTCTGGCGGCGCAAAAAACGCTGGTGGATGTTATCCGCAAAGAGGTTGCGGCTGAAGGCGGTAGCGTGCTGCTACTTTCCGGTGGCGACATTAACACTGGCGTGCCCGAGTc                                                        <  2:681192/140‑1 (MQ=255)                                                                                 ttGGCGCAATGAATATGGCGAATATGGTCTGGCGGCGCAAAAAACGCTGGTGGATGTTATCCGCAAAGAGGTTGCGGCTGAAGGCGGTAGCGTGCTGCTACTTTCCGGTGGCGACATTAACACTGGCGTGCCCGAGTCTg                                                      <  2:754489/140‑1 (MQ=255)                                                                                   ggCGCAATGAATATGGCGAATATGGTCTGGCGGCGCAAAAAACGCTGGTGGATGTTATCCGCAAAGAGGTTGCGGCTGAAGGCGGTAGCGTGCTGCTACTTTCCGGTGGCGACATTAACACTGGCGTGCCCGAGTCTGAc                                                    <  2:542917/140‑1 (MQ=255)                                                                                             atatGGCGAATATGGTCTGGCGGCGCAAAAAACGCTGGTGGATGTTATCCGCAAAGAGGTTGCGGCTGAAGGCGGTAGCGTGCTGCTACTTTCCGGTGGCGACATTAACACTGGCGTGCCCGAGTCTGACTTACAGGATg                                          <  2:1262160/140‑1 (MQ=255)                                                                                                   cGAATATGGTCTGGCGGCGCAAAAAACGCTGGTGGATGTTATCCGCAAAGAGGTTGCGGCTGAAGGCGGTAGCGTGCTGCTACTTTCCGGTGGCGACATTAACACTGGCGTGCCCGAGTCTGACTTACAGGATGCCGAAc                                    >  1:772338/1‑140 (MQ=255)                                                                                                      atatGGTCTGGCGGCGCAAAAAACGCTGGTGGATGTTATCCGCAAAGAGGTTGCGGCTGAAGGCGGTAGCGTGCTGCTACTTTCCGGTGGCGACATTAACACTGGCGTGCCCGAGTCTGACTTACAGGATGCCGAACCTg                                 >  1:1090597/1‑140 (MQ=255)                                                                                                      atatGGTCTGGCGGCGCAAAAAACGCTGGTGGATGTTATCCGCAAAGAGGTTGCGGCTGAAGGCGGTAGCGTGCTGCTACTTTCCGGTGGCGACATTAACACTGGCGTGCCCGAGTCTGACTTACAGGATGCCGAACCTg                                 >  1:948274/1‑140 (MQ=255)                                                                                                      atatGGTCTGGCGGCGCAAAAAACGCTGGTGGATGTTATCCGCAAAGAGGTTGCGGCTGAAGGCGGTAGCGTGCTGCTACTTTCCGGTGGCGACATTAACACTGGCGTGCCCGAGTCTGACTTACAGGATGCCGAACCTg                                 >  2:544877/1‑140 (MQ=255)                                                                                                        atGGTCTGGCGGCGCAAAAAACGCTGGTGGATGTTATCCGCAAAGAGGTTGCGGCTGAAGGCGGTAGCGTGCTGCTACTTTCCGGTGGCGACATTAACACTGGCGTGCCCGAGTCTGACTTACAGGATGCCGAACCTGAt                               <  1:551936/140‑1 (MQ=255)                                                                                                        atGGTCTGGCGGCGCAAAAAACGCTGGTGGATGTTATCCGCAAAGAGGTTGCGGCTGAAGGCGGTAGCGTGCTGCTACTTTCCGGTGGCGACATTAACACTGGCGTGCCCGAGTCTGACTTACAGGATGCCGAACCTGAt                               >  2:1159229/1‑140 (MQ=255)                                                                                                            tCTGGCGGCGCAAAAAACGCTGGTGGATGTTATCCGCAAAGAGGTTGCGGCTGAAGGCGGTAGCGTGCTGCTACTTTCCGGTGGCGACATTAACACTGGCGTGCCCGAGTCTGACTTACAGGATGCCGAACCTGATTTTc                           >  2:888624/1‑140 (MQ=255)                                                                                                            tCTGGCGGCGCAAAAAACGCTGGTGGATGTTATCCGCAAAGAGGTTGCGGCTGAAGGCGGTAGCGTGCTGCTACTTTCCGGTGGCGACATTAACACTGGCGTGCCCGAGTCTGACTTACAGGATGCCGAACCTGATTTTc                           <  1:690863/140‑1 (MQ=255)                                                                                                             cTGGCGGCGCAAAAAACGCTGGTGGATGTTATCCGCAAAGAGGTTGCGGCTGAAGGCGGTAGCGTGCTGCTACTTTCCGGTGGCGACATTAACACTGGCGTGCCCGAGTCTGACTTACAGGATGCCGAACCTGATTTTcg                          <  2:90571/140‑1 (MQ=255)                                                                                                             cTGGCGGCGCAAAAAACGCTGGTGGATGTTATCCGCAAAGAGGTTGCGGCTGAAGGCGGTAGCGTGCTGCTACTTTCCGGTGGCGACATTAACACTGGCGTGCCCGAGTCTGACTTACAGGATGCCGAACCTGATTTTcg                          <  2:948274/140‑1 (MQ=255)                                                                                                             cTGGCGGCGCAAAAAACGCTGGTGGATGTTATCCGCAAAGAGGTTGCGGCTGAAGGCGGTAGCGTGCTGCTACTTTCCGGTGGCGACATTAACACTGGCGTGCCCGAGTCTGACTTACAGGATGCCGAACCTGATTTTcg                          <  2:400430/140‑1 (MQ=255)                                                                                                             cTGGCGGCGCAAAAAACGCTGGTGGATGTTATCCGCAAAGAGGTTGCGGCTGAAGGCGGTAGCGTGCTGCTACTTTCCGGTGGCGACATTAACACTGGCGTGCCCGAGTCTGACTTACAGGATGCCGAACCTGATTTTcg                          <  1:431441/140‑1 (MQ=255)                                                                                                             cTGGCGGCGCAAAAAACGCTGGTGGATGTTATCCGCAAAGAGGTTGCGGCTGAAGGCGGTAGCGTGCTGCTACTTTCCGGTGGCGACATTAACACTGGCGTGCCCGAGTCTGACTTACAGGATGCCGAACCTGATTTTcg                          <  2:1090597/140‑1 (MQ=255)                                                                                                             cTGGCGGCGCAAAAAACGCTGGTGGATGTTATCCGCAAAGAGGTTGCGGCTGAAGGCGGTAGCGTGCTGCTACTTTCCGGTGGCGACATTAACACTGGCGTGCCCGAGTCTGACTTACAGGATGCCGAACCTGATTTTcg                          <  2:1374636/140‑1 (MQ=255)                                                                                                               ggcggcGCAAAAAACGCTGGTGGATGTTATCCGCAAAGAGGTTGCGGCTGAAGGCGGTAGCGTGCTGCTACTTTCCGGTGGCGACATTAACACTGGCGTGCCCGAGTCTGACTTACAGGATGCCGAACCTGATTTTcgcg                        >  1:1347590/1‑140 (MQ=255)                                                                                                                gcggcgCAAAAAACGCTGGTGGATGTTATCCGCAAAGAGGTTGCGGCTGAAGGCGGTAGCGTGCTGCTACTTTCCGGTGGCGACATTAACACTGGCGTGCCCGAGTCTGACTTACAGGATGCCGAACCTGATTTTCGCgg                       <  1:1037514/140‑1 (MQ=255)                                                                                                                gcggcgCAAAAAACGCTGGTGGATGTTATCCGCAAAGAGGTTGCGGCTGAAGGCGGTAGCGTGCTGCTACTTTCCGGTGGCGACATTAACACTGGCGTGCCCGAGTCTGACTTACAGGATGCCGAACCTGATTTTCGCgg                       >  1:221380/1‑140 (MQ=255)                                                                                                                gcggcgCAAAAAACGCTGGTGGATGTTATCCGCAAAGAGGTTGCGGCTGAAGGCGGTAGCGTGCTGCTACTTTCCGGTGGCGACATTAACACTGGCGTGCCCGAGTCTGACTTACAGGATGCCGAACCTGATTTTCGCgg                       <  1:278326/140‑1 (MQ=255)                                                                                                                 cggcgCAAAAAACGCTGGTGGATGTTATCCGCAAAGAGGTTGCGGCTGAAGGCGGTAGCGTGCTGCTACTTTCCGGTGGCGACATTAACACTGGCGTGCCCGAGTCTGACTTACAGGATGCCGAACCTGATTTTCGCGGt                      <  2:1281681/140‑1 (MQ=255)                                                                                                                    cgcAAAAAACGCTGGTGGATGTTATCCGCAAAGAGGTTGCGGCTGAAGGCGGTAGCGTGCTGCTACTTTCCGGTGGCGACATTAACACTGGCGTGCCCGAGTCTGACTTACAGGATGCCGAACCTGATTTTCGCGGTATg                   >  1:1068693/1‑140 (MQ=255)                                                                                                                     gcAAAAAACGCTGGTGGATGTTATCCGCAAAGAGGTTGCGGCTGAAGGCGGTAGCGTGCTGCTACTTTCCGGTGGCGACATTAACACTGGCGTGCCCGAGTCTGACTTACAGGATGCCGAACCTGATTTTCGCGGTATGa                  >  2:1296819/1‑140 (MQ=255)                                                                                                                      cAAAAAACGCTGGTGGATGTTATCCGCAAAGAGGTTGCGGCTGAAGGCGGTAGCGTGCTGCTACTTTCCGGTGGCGACATTAACACTGGCGTGCCCGAGTCTGACTTACAGGATGCCGAACCTGATTTTCGCGGTATGaa                 >  1:407368/1‑140 (MQ=255)                                                                                                                      cAAAAAACGCTGGTGGATGTTATCCGCAAAGAGGTTGCGGCTGAAGGCGGTAGCGTGCTGCTACTTTCCGGTGGCGACATTAACACTGGCGTGCCCGAGTCTGACTTACAGGATGCCGAACCTGATTTTCGCGGTATGaa                 >  1:803613/1‑140 (MQ=255)                                                                                                                          aaaCGCTGGTGGATGTTATCCGCAAAGAGGTTGCGGCTGAAGGCGGTAGCGTGCTGCTACTTTCCGGTGGCGACATTAACACTGGCGTGCCCGAGTCTGACTTACAGGATGCCGAACCTGATTTTCGCGGTATGAATCtg             <  1:888624/140‑1 (MQ=255)                                                                                                                            aCGCTGGTGGATGTTATCCGCAAAGAGGTTGCGGCTGAAGGCGGTAGCGTGCTGCTACTTTCCGGTGGCGACATTAACACTGGCGTGCCCGAGTCTGACTTACAGGATGCCGAACCTGATTTTCGCGGTATGAATCtggt           <  1:1159229/140‑1 (MQ=255)                                                                                                                                 ggtggATGTTATCCGCAAAGAGGTTGCGGCTGAAGGCGGTAGCGTGCTGCTACTTTCCGGTGGCGACATTAACACTGGCGTGCCCGAGTCTGACTTACAGGATGCCGAACCTGATTTTCGCGGTATGa                  >  2:87192/1‑128 (MQ=255)                                                                                                                                 ggtggATGTTATCCGCAAAGAGGTTGCGGCTGAAGGCGGTAGCGTGCTGCTACTTTCCGGTGGCGACATTAACACTGGCGTGCCCGAGTCTGACTTACAGGATGCCGAACCTGATTTTCGCGGTATGa                  <  1:87192/128‑1 (MQ=255)                                                                                                                                  gtggATGTTATCCGCAAAGAGGTTGCGGCTGAAGGCGGTAGCGTGCTGCTACTTTCCGGTGGCGACATTAACACTGGCCTGCCCGAGTCTGACTTACAGGATGCCGAACCTGATTTTCGCGGTATGAATCTGGTGGGCTa     <  2:803613/140‑1 (MQ=255)                                                                                                                                     gATGTTATCCGCAAAGAGGTTGCGGCTGAAGGCGGTAGCGTGCTGCTACTTTCCGGTGGCGACATTAACACTGGCGTGCCCGAGTCTGACTTACAGGATGCCGAACCTGATTTTCGCGGTATGAATCTGGTGGGCTATGa  <  2:1347590/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                        AGTGAAACTGCTCTGGCGTATGAGCAGGATAAAACCTACAAAATTACAGTTCTGCATACCAATGATCATCATGGGCATTTTTGGCGCAATGAATATGGCGAATATGGTCTGGCGGCGCAAAAAACGCTGGTGGATGGTATCCGCAAAGAGGTTGCGGCTGAAGGCGGTAGCGTGCTGCTACTTTCCGGTGGCGACATTAACACTGGCGTGCCCGAGTCTGACTTACAGGATGCCGAACCTGATTTTCGCGGTATGAATCTGGTGGGCTATGA  >  NC_000913/504971‑505242

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑