Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,068,941	C→G	G300A (GGC→GCC)	rutG ←	pyrimidine:H(+) symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,068,941	0	C	G	100.0%	233.9 / NA	60	G300A (GGC→GCC)	rutG	pyrimidine:H(+) symporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (26/34);  total (26/34)

AATCAGCGCACCAAATTTCGGTGAAAAGCCCAACAGCATGGCGATGACGGCAGCAGCAACAAACACCAGCGTCGAGTAGACTTTGGTCACGGCCATCACACCGATATTTTCAGCATAGGTGGTCACGCCGCTACCGCCGACAGAGCCGGAAAGCATCGTTGCCAGACCATCGCCTACGAATGCCCGCCCCATATACGGGTCCATATTGCGTCCGGTCATCCCGGCGACTGCCTTGAGATGACCTAAGTTTTCCGCCACCAGAATCACCGC  >  NC_000913/1068805‑1069074                                                                                                                                         |                                                                                                                                      aaTCAGCGCACCAAATTTCGGTGAAAAGCCCAACAGCATGGCGATGACGGCAGCAGCAACAAACACCAGCGTCGAGTAGACTTTGGTCACGGCCATCACACCGATATTTTCAGCATAGGTGGTCACGCCGCTACCGGCGa                                                                                                                                    <  1:864009/140‑1 (MQ=255) aaTCAGCGCACCAAATTTCGGTGAAAAGCCCAACAGCATGGCGATGACGGCAGCAGCAACAAACACCAGCGTCGAGTAGACTTTGGTCACGGCCATCACACCGATATTTTCAGCATAGGTGGTCACGCCGCTACCGGCGa                                                                                                                                    <  1:210460/140‑1 (MQ=255)  aTCAGCGCACCAAATTTCGGTGAAAAGCCCAACAGCATGGCGATGACGGCAGCAGCAACAAACACCAGCGTCGAGTAGACTTTGGTCACGGCCATCACACCGATATTTTCAGCATAGGTGGTCACGCCGCTACCGGCGAc                                                                                                                                   >  1:666733/1‑140 (MQ=255)  aTCAGCGCACCAAATTTCGGTGAAAAGCCCAACAGCATGGCGATGACGGCAGCAGCAACAAACACCAGCGTCGAGTAGACTTTGGTCACGGCCATCACACCGATATTTTCAGCATAGGTGGTCACGCCGCTACCGGCGAc                                                                                                                                   >  2:1366124/1‑140 (MQ=255)  aTCAGCGCACCAAATTTCGGTGAAAAGCCCAACAGCATGGCGATGACGGCAGCAGCAACAAACACCAGCGTCGAGTAGACTTTGGTCACGGCCATCACACCGATATTTTCAGCATAGGTGGTCACGCCGCTACCGGCGAc                                                                                                                                   >  2:242060/1‑140 (MQ=255)  aTCAGCGCACCAAATTTCGGTGAAAAGCCCAACAGCATGGCGATGACGGCAGCAGCAACAAACACCAGCGTCGAGTAGACTTTGGTCACGGCCATCACACCGATATTTTCAGCATAGGTGGTCACGCCGCTACCGGCGAc                                                                                                                                   >  2:402269/1‑140 (MQ=255)     aGCGCACCAAATTTCGGTGAAAAGCCCAACAGCATGGCGATGACGGCAGCAACAACAAACACCAGCGTCGAGTAGACTTTGGTCACGGCCATCACACCGATATTTTCAGCATAGGTGGTCACGCCGCTACCGGCGACaga                                                                                                                                >  2:435643/1‑140 (MQ=255)      gcgcACCAAATTTCGGTGAAAAGCCCAACAGCATGGCGATGACGGCAGCAGCAACAAACACCAGCGTCGAGTAGACTTTGGTCACGGCCATCACACCGATATTTTCAGCATAGGTGGTCACGCCGCTACCGGCGACagag                                                                                                                               <  2:335055/140‑1 (MQ=255)      gcgcACCAAATTTCGGTGAAAAGCCCAACAGCATGGCGATGACGGCAGCAGCAACAAACACCAGCGTCGAGTAGACTTTGGTCACGGCCATCACACCGATATTTTCAGCATAGGTGGTCACGCCGCTACCGGCGACag                                                                                                                                 >  2:848416/1‑138 (MQ=255)      gcgcACCAAATTTCGGTGAAAAGCCCAACAGCATGGCGATGACGGCAGCAGCAACAAACACCAGCGTCGAGTAGACTTTGGTCACGGCCATCACACCGATATTTTCAGCATAGGTGGTCACGCCGCTACCGGCGACag                                                                                                                                 <  1:848416/138‑1 (MQ=255)      gcgcACCAAATTTCGGTGAAAAGCCCAACAGCATGGCGATGACGGCAGCAGCAACAAACACCAGCGTCGAGTAGACTTTGGTC‑CGGCCATCACACCGATATTTTCAGCATAGGTGGTCACGCCGCTACCGGCGa                                                                                                                                    <  2:1170850/134‑1 (MQ=255)      gcgcACCAAATTTCGGTGAAAAGCCCAACAGCATGGCGATGACGGCAGCAGCAACAAACACCAGCGTCGAGTAGACTTTGGTC‑CGGCCATCACACCGATATTTTCAGCATAGGTGGTCACGCCGCTACCGGCGa                                                                                                                                    >  1:1170850/1‑134 (MQ=255)       cgcACCAAATTTCGGTGAAAAGCCCAACAGCATGGCGATGACGGCAGCAGCAACAAACACCAGCGTCGAGTAGACTTTGGTCACGGCCATCACACCGATATTTTCAGCATAGGTGGTCACGCCGCTACCGGCGACAGAGc                                                                                                                              >  1:280132/1‑140 (MQ=255)       cgcACCAAATTTCGGTGAAAAGCCCAACAGCATGGCGATGACGGCAGCAGCAACAAACACCAGCGTCGAGTAGACTTTGGTCACGGCCATCACACCGATATTTTCAGCATAGGTGGTCACGCCGCTACCGGCGACAGAGc                                                                                                                              >  2:749606/1‑140 (MQ=255)       cgcACCAAATTTCGGTGAAAAGCCCAACAGCATGGCGATGACGGCAGCAGCAACAAACACCAGCGTCGAGTAGACTTTGGTCACGGCCATCACACCGATATTTTCAGCATAGGTGGTCACGCCGCTACCGGCGACAGAGc                                                                                                                              >  2:1293331/1‑140 (MQ=255)         cACCAAATTTCGGTGAAAAGCCCAACAGCATGGCGATGACGGCAGCAGCAACAAACACCAGCGTCGAGTAGACTTTGGTCACGGCCATCACACCGATATTTTCAGCATAGGTGGTCACGCCGCTACCGGCGACAGAGCCg                                                                                                                            <  1:749606/140‑1 (MQ=255)           ccAAATTTCGGTGAAAAGCCCAACAGCATGGCGATGACGGCAGCAGCAACAAACACCAGCGTCGAGTAGACTTTGGTCACGGCCATCACACCGATATTTTCAGCATAGGTGGTCACGCCGCTACCGGCGACAGAGCCGGa                                                                                                                          <  1:242060/140‑1 (MQ=255)              aaTTTCGGTGAAAAGCCCAACAGCATGGCGATGACGGCAGCAGCAACAAACACCAGCGTCGAGTAGACTTTGGTCACGGCCATCACACCGATATTTTCAGCATAGGTGGTCACGCCGCTACCGGCGACAGAGCCGGAAAg                                                                                                                       <  1:1293331/140‑1 (MQ=255)                 ttCGGTGAAAAGCCCAACAGCATGGCGATGACGGCAGCAGCAACAAACACCAGCGTCGAGTAGACTTTGGTCACGGCCATCACACCGATATTTTCAGCATAGGTGGTCACGCCGCTACCGGCGACAGAGCCGGAAAGCAt                                                                                                                    <  2:106309/140‑1 (MQ=255)                  tCGGTGAAAAGCCCAACAGCATGGCGATGACGGCAGCAGCAACAAACACCAGCGTCGAGTAGACTTTGGTCACGGCCATCACACCGATATTTTCAGCATAGGTGGTCACGCCGCTACCGGCGACAGAGCCGGAAAGCATc                                                                                                                   <  1:674553/140‑1 (MQ=255)                            ccccAACAGCATGGCGATGACGGCAGCAGCAACAAACACCAGCGTCGAGTAGACTTTGGTCACGGCCATCACACCGATATTTTCAGCATAGGTGGTCACGCCGCTACCGGCGACAGAGCCGGAAAGCATCGTTGCCAGAc                                                                                                         <  1:706630/139‑1 (MQ=255)                               cAACAGCATGGCGATGACGGCAGCAGCAACAAACACCAGCGTCGAGTAGACTTTGGTCACGGCCATCACACCGATATTTTCAGCATAGGTGGTCACGCCGCTACCGGCGACAGAGCCGGAAAGCATCGTTGCCAGACCAt                                                                                                      <  2:1308034/140‑1 (MQ=255)                                  cAGCATGGCGATGACGGCAGCAGCAACAAACACCAGCGTCGAGTAGACTTTGGTCACGGCCATCACACCGATATTTTCAGCATAGGTGGTCACGCCGCTACCGGCGACAGAGCCGGAAAGCATCGTTGCCAGACCATCGc                                                                                                   >  1:901433/1‑140 (MQ=255)                                   aGCATGGCGATGACGGCAGCAGCAACAAACACCAGCGTCGAGTAGACTTTGGTCACGGCCATCACACCGATATTTTCAGCATAGGTGGTCACGCCGCTACCGGCGACAGAGCCGGAAAGCATCGTTGCCAGACCATCGcc                                                                                                  <  2:280132/140‑1 (MQ=255)                                       tGGCGATGACGGCAGCAGCAACAAACACCAGCGTCGAGTAGACTTTGGTCACGGCCATCACACCGATATTTTCAGCATAGGTGGTCACGCCGCTACCGGCGACAGAGCCGGAAAGCATCGTTGCCAGACCATCGCCTACg                                                                                              >  1:1350453/1‑140 (MQ=255)                                             tGACGGCAGCAGCAACAAACACCAGCGTCGAGTAGACTTTGGTCACGGCCATCACACCGATATTTTCAGCATAGGTGGTCACGCCGCTACCGGCGACAGAGCCGGAAAGCATCGTTGCCAGACCATCGCCTACGAATgcc                                                                                        <  2:1350453/140‑1 (MQ=255)                                             tGACGGCAGCAGCAACAAACACCAGCGTCGAGTAGACTTTGGTCACGGCCATCACACCGATATTTTCAGCATAGGTGGTCACGCCGCTACCGGCGACAGAGCCGGAAAGCATCGTTGCCAGACCATCGCCTACGAATgcc                                                                                        <  1:36629/140‑1 (MQ=255)                                              gACGGTAGCAGCAACAAACACCAGCGTCGAGTAGACTTTGGTCACGGCCATCACACCGATATTTTCAGCATAGGTGGTCACGCCGCTACCGGCGACAGAGCCGGAAAGCATCGTTGCCAGACCATCGCCTACGAATgccc                                                                                       <  1:1276153/140‑1 (MQ=255)                                               ccGGCAGCAGCAACAAACACCAGCGTCGAGTAGCCTTTGGTCACGGTCATCACCCCGATATTTTCAGCATAGGTGGTCACGCCGCTACCGGCGACAGAGCCGGAAAGCATCGTTGCCAGACCATCGCCTACGAATgcccg                                                                                      <  2:1240029/139‑1 (MQ=255)                                               aCGGCAGCAGCAACAAACACCAGCGTCGAGTAGACTTTGGTCACGGCCATCACACCGATATTTTCAGCATAGGTGGTCACGCCGCTACCGGCGACAGAGCCGGAAAGCATCGTTGCCAGACCATCGCCTACGAATgcccg                                                                                      <  1:821302/140‑1 (MQ=255)                                                cGGCAGCAACAACAAACACCAGCGTCGAGTAGACTTTGGTCACGGCCATCACACCGATATTTTCAGCATAGGTGGTCACGCCGCTACCGGCGACAGAGCCGGAAAGCATCGTTGCCAGACCATCGCCTACGAATgcccgc                                                                                     <  1:435643/140‑1 (MQ=255)                                                 ggCAGCAGCAACAAACACCAGCGTCGAGTAGACTTTGGTCACGGCCATCACACCGATATTTTCAGCATAGGTGGTCACGCCGCTACCGGCGACAGAGCCGGAAAGCATCGTTGCCAGACCATCGCCTACGAATgcccgcc                                                                                    <  2:666733/140‑1 (MQ=255)                                                 ggCAGCAGCAACAAACACCAGCGTCGAGTAGACTTTGGTCACGGCCATCACACCGATATTTTCAGCATAGGTGGTCACGCCGCTACCGGCGACAGAGCCGGAAAGCATCGTTGCCAGACCATCGCCTACGAATgcccgcc                                                                                    <  1:1343731/140‑1 (MQ=255)                                                          aacaaacaCCAGCGTCGAGTAGACTTTGGTCACGGCCATCACACCGATATTTTCAGCATAGGTGGTCACGCCGCTACCGGCGACAGAGCCGGAAAGCATCGTTGCCAGACCATCGCCTACGAATGCCCGCCCCATATACg                                                                           <  1:1014418/140‑1 (MQ=255)                                                            caaacaCCAGCGTCGAGTAGACTTTGGTCACGGCCATCACACCGATATTTTCAGCATAGGTGGTCACGCCGCTACCGGCGACAGAGCCGGAAAGCATCGTTGCCAGACCATCGCCTACGAATGCCCGCCCCATATACggg                                                                         <  1:501949/140‑1 (MQ=255)                                                                   cAGCGTCGAGTAGACTTTGGTCACGGCCATCACACCGATATTTTCAGCATAGGTGGTCACGCCGCTACCGGCGACAGAGCCGGAAAGCATCGTTGCCAGACCATCGCCTACGAATGCCCGCCCCATATACGGGTCCatat                                                                  >  1:69741/1‑140 (MQ=255)                                                                      cGTCGAGTAGACTTTGGTCACGGCCATCACACCGATATTTTCAGCATAGGTGGTCACGCCGCTACCGGCGACAGAGCCGGAAAGCATCGTTGCCAGACCATCGCCTACGAATGCCCGCCCCATATACGGGTCCATATTGc                                                               >  2:974871/1‑140 (MQ=255)                                                                       gTCGAGTAGACTTTGGTCACGGCCATCACACCGATATTTTCAGCATAGGTGGTCACGCCGCTACCGGCGACAGAGCCGGAAAGCATCGTTGCCAGACCATCGCCTACGAATGCCCGCCCCATATACGGGTCCATATTGCg                                                              <  2:1131775/140‑1 (MQ=255)                                                                                aCTTTGGTCACGGCCATCACACCGATATTTTCAGCATAGGTGGTCACGCCGCTACCGGCGACAGAGCCGGAAAGCATCGTTGCCAGACCATCGCCTACGAATGCCCGCCCCATATACGGGTCCATATTGCGTCCGGTCAt                                                     >  2:1112139/1‑140 (MQ=255)                                                                                        cACGGCCATCACACCGATATTTTCAGCATAGGTGGTCACGCCGCTACCGGCGACAGAGCCGGAAAGCATCGTTGCCAGACCATCGCCTACGAATGCCCGCCCCATATACGGGTCCATATTGCGTCCGGTCATCCCGGCGa                                             <  2:361891/140‑1 (MQ=255)                                                                                        cACGGCCATCACACCGATATTTTCAGCATAGGTGGTCACGCCGCTACCGGCGACAGAGCCGGAAAGCATCGTTGCCAGACCATCGCCTACGAATGCCCGCCCCATATACGGGTCCATATTGCGTCCGGTCATCCCGGCGa                                             <  1:1212390/140‑1 (MQ=255)                                                                                        cACGGCCATCACACCGATATTTTCAGCATAGGTGGTCACGCCGCTACCGGCGACAGAGCCGGAAAGCATCGTTGCCAGACCATCGCCTACGAATGCCCGCCCCATATACGGGTCCATATTGCGTCCGGTCATCCCGGCGa                                             <  2:1151504/140‑1 (MQ=255)                                                                                          cGGCCATCACACCGATATTTTCAGCATAGGTGGTCACGCCGCTACCGGCGACAGAGCCGGAAAGCATCGTTGCCAGACCATCGCCTACGAATGCCCGCCCCATATACGGGTCCATATTGCGTCCGGTCATCCCGGCGACt                                           <  2:1182166/140‑1 (MQ=255)                                                                                          cGGCCATCACACCGATATTTTCAGCATAGGTGGTCACGCCGCTACCGGCGACAGAGCCGGAAAGCATCGTTGCCAGACCATCGCCTACGAATGCCCGCCCCATATACGGGTCCATATTGCGTCCGGTCATCCCGGCGACt                                           >  2:499665/1‑140 (MQ=255)                                                                                             ccATCACACCGATATTTTCAGCATAGGTGGTCACGCCGCTACCGGCGACAGAGCCGGAAAGCATCGTTGCCAGACCATCGCCTACGAATGCCCGCCCCATATACGGGTCCATATTGCGTCCGGTCATCCCGGCGACTGcc                                        <  1:499111/140‑1 (MQ=255)                                                                                                  acacCGATATTTTCAGCATAGGTGGTCACGCCGCTACCGGCGACAGAGCCGGAAAGCATCGTTGCCAGACCATCGCCTACGAATGCCCGCCCCATATACGGGTCCATATTGCGTCCGGTCATCCCGGCGACTGCCTTgag                                   <  1:402269/140‑1 (MQ=255)                                                                                                       gATATTTTCAGCATAGGTGGTCACGCCGCTACCGGCGACAGAGCCGGAAAGCATCGTTGCCAGACCATCGCCTACGAATGCCCGCCCCATATACGGGTCCATATTGCGTCCGGTCATCCCGGCGACTGCCTTGAGATGAc                              <  2:69741/140‑1 (MQ=255)                                                                                                            tttCAGCATAGGTGGTCACGCCGCTACCGGCGACAGAGCCGGAAAGCATCGTTGCCAGACCATCGCCTACGAATGCCCGCCCCATATACGGGTCCATATTGCGTCCGGTCATCCCGGCGACTGCCTTGAGATGACCTAAg                         >  1:194530/1‑140 (MQ=255)                                                                                                                  cATAGGTGGTCACGCCGCTACCGGCGACAGAGCCGGAAAGCATCGTTGCCAGACCATCGCCTACGAATGCCCGCCCCATATACGGGTCCATATTGCGTCCGGTCATCCCGGCGACTGCCTTGAGATGACCTAAGTTTTcc                   >  1:918318/1‑140 (MQ=255)                                                                                                                   aTAGGTGGTCACGCCGCTACCGGCGACAGAGCCGGAAAGCATCGTTGCCAGACCATCGCCTACGAATGCCCGCCCCATATACGGGTCCATATTGCGTCCGGTCATCCCGGCGACTGCCTTGAGATGACCTAAGTTTTCCg                  <  2:828229/140‑1 (MQ=255)                                                                                                                            cACGCCGCTACCGGCGACAGAGCCGGAAAGCATCGTTGCCAGACCATCGCCTACGAATGCCCGCCCCATATACGGGTCCATATTGCGTCCGGTCATCCCGGCGACTGCCTTGAGATGACCTAAGTTTTCCGCCACCAGaa         >  1:135316/1‑140 (MQ=255)                                                                                                                            cACGCCGCTACCGGCGACAGAGCCGGAAAGCATCGTTGCCAGACCATCGCCTACGAATGCCCGCCCCATATACGGGTCCATATTGCGTCCGGTCATCCCGGCGACTGCCTTGAGATGACCTAAGTTTTCCGCCACCAGaa         >  1:455080/1‑140 (MQ=255)                                                                                                                            cACGCCGCTACCGGCGACAGAGCCGGAAAGCATCGTTGCCAGACCATCGCCTACGAATGCCCGCCCCATATACGGGTCCATATTGCGTCCGGTCATCCCGGCGACTGCCTTGAGATGACCTAAGTTTTCCGCCACCAGaa         >  2:903709/1‑140 (MQ=255)                                                                                                                             aCGCCGCTACCGGCGACAGAGCCGGAAAGCATCGTTGCCAGACCATCGCCTACGAATGCCCGCCCCATATACGGGTCCATATTGCGTCCGGTCATCCCGGCGACTGCCTTGAGATGACCTAAGTTTTCCGCCACCAGAAt        <  1:1112139/140‑1 (MQ=255)                                                                                                                               gccgcTACCGGCGACAGAGCCGGAAAGCATCGTTGCCAGACCATCGCCTACGAATGCCCGCCCCATATACGGGTCCATATTGCGTCCGGTCATCCCGGCGACTGCCTTGAGATGACCTAAGTTTTCCg                  <  1:1327667/128‑1 (MQ=255)                                                                                                                               gccgcTACCGGCGACAGAGCCGGAAAGCATCGTTGCCAGACCATCGCCTACGAATGCCCGCCCCATATACGGGTCCATATTGCGTCCGGTCATCCCGGCGACTGCCTTGAGATGACCTAAGTTTTCCg                  >  2:1327667/1‑128 (MQ=255)                                                                                                                                 cgcTACCGGCGACAGAGCCGGAAAGCATCGTTGCCAGACCATCGCCTACGAATGCCCGCCCCATATACGGGTCCATATTGCGTCCGGTCATCCCGGCGACTGCCTTGAGATGACCTAAGTTTTc                    >  1:1137435/1‑124 (MQ=255)                                                                                                                                 cgcTACCGGCGACAGAGCCGGAAAGCATCGTTGCCAGACCATCGCCTACGAATGCCCGCCCCATATACGGGTCCATATTGCGTCCGGTCATCCCGGCGACTGCCTTGAGATGACCTAAGTTTTc                    <  2:1137435/124‑1 (MQ=255)                                                                                                                                 cgcTACCGGCGACAGAGCCGGAAAGCATCGTTGCCAGACCATCGCCTACGAATGCCCGCCCCATATACGGGTCCATATTGCGTCCGGTCATCCCGGCGACTGCCTTGAGATGACCTAAGTTTTCCGCCACCAGAATCAcc    >  2:610152/1‑140 (MQ=255)                                                                                                                                  gcTACCGGCGACAGAGCCGGAAAGCATCGTTGCCAGACCATCGCCTACGAATGCCCGCCCCATATACGGGTCCATATTGCGTCCGGTCATCCCGGCGACTGCCTTGAGATGACCTAAGTTTTCCGCCACCAGAATCACCg   >  1:961988/1‑140 (MQ=255)                                                                                                                                   cTACCGGCGACAGAGCCGGAAAGCATCGTTGCCAGACCATCGCCTACGAATGCCCGCCCCATATACGGGTCCATATTGCGTCCGGTCATCCCGGCGACTGCCTTGAGATGACCTAAGTTTTCCGCCACCAGAATCACCGc  >  1:1003087/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                      AATCAGCGCACCAAATTTCGGTGAAAAGCCCAACAGCATGGCGATGACGGCAGCAGCAACAAACACCAGCGTCGAGTAGACTTTGGTCACGGCCATCACACCGATATTTTCAGCATAGGTGGTCACGCCGCTACCGCCGACAGAGCCGGAAAGCATCGTTGCCAGACCATCGCCTACGAATGCCCGCCCCATATACGGGTCCATATTGCGTCCGGTCATCCCGGCGACTGCCTTGAGATGACCTAAGTTTTCCGCCACCAGAATCACCGC  >  NC_000913/1068805‑1069074

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑