Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,339,171	C→G	A84P (GCG→CCG)	gyrA ←	DNA gyrase subunit A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,339,171	0	C	G	100.0%	236.3 / NA	61	A84P (GCG→CCG)	gyrA	DNA gyrase subunit A
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (28/33);  total (28/33)

TTCGCCAGACGGATTTCCGTATAACGCATTGCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGCCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGACGGTGTACCGGCTTCAGGCCATC  >  NC_000913/2339035‑2339306                                                                                                                                         |                                                                                                                                        ttCGCCAGACGGATTTCCGTATAACGCATTGCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGGCGa                                                                                                                                      <  1:691509/140‑1 (MQ=255)   cGCCAGACGGATTTCCGTATAACGCATTGCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACTAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTAATAGACCGGCGAGt                                                                                                                                    <  2:554755/140‑1 (MQ=255)   cGCCAGACGGATTTCCGTATAACGCATTGCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGGCGAGt                                                                                                                                    <  2:654873/140‑1 (MQ=255)    gCCAGACGGATTTCCGTATAACGCATTGCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGGCGAGTc                                                                                                                                   <  1:648005/140‑1 (MQ=255)      cAGACGGATTTCCGTATAACGCATTGCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGGCGAGTCAc                                                                                                                                 <  1:842524/140‑1 (MQ=255)         aCGGATTTCCGTATAACGCATTGCCGCCGCAGAGTCGCCGTCGATAGAATCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGGCGAGTCACCAt                                                                                                                              <  2:303581/140‑1 (MQ=255)             aTTTCCGTATAACGCATTGCCGCCGCAGAGTCGCCGTCGATAGACCCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGGCGAGTCACCATgggg                                                                                                                          <  2:158434/140‑1 (MQ=255)             aTTTCCGTATAACGCATTGCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGGCGAGTCACCATgggg                                                                                                                          <  1:184933/140‑1 (MQ=255)              tttCCGTATAACGCATTGCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGGCGAGTCACCATGGGGa                                                                                                                         <  1:799958/140‑1 (MQ=255)                    tataACGCATTGCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGGCGAGTCACCATgggg                                                                                                                          <  1:852027/133‑1 (MQ=255)                    tataACGCATTGCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGGCGAGTCACCATgggg                                                                                                                          >  2:852027/1‑133 (MQ=255)                         cGCATTGCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGGCGAGTCACCATGGGGATGGTATTTAcc                                                                                                              >  1:259024/1‑140 (MQ=255)                         cGCATTGCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGGCGAGTCACCATGGGGATGGTATTTAcc                                                                                                              >  1:393792/1‑140 (MQ=255)                          gCATTGCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGGCGAGTCACCATGGGGATGGTATTTACCg                                                                                                             <  2:259024/140‑1 (MQ=255)                                   ccgcAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGGCGAGTCACCATGGGGATGGTATTTACCGATTACGTCa                                                                                                    >  1:406130/1‑140 (MQ=255)                                    cgcAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGGCGAGTCACCATGGGGATGGTATTTACCTATTACGTCAc                                                                                                   >  1:410793/1‑140 (MQ=255)                                           tCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGGCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGac                                                                                            >  1:819563/1‑140 (MQ=255)                                                   gATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGGCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAg                                                                                    >  2:388984/1‑140 (MQ=255)                                                           cGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGGCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTta                                                                            >  1:76720/1‑140 (MQ=255)                                                            gAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGGCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTtat                                                                           <  2:819563/140‑1 (MQ=255)                                                               gTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGGCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAgg                                                                        >  1:142067/1‑140 (MQ=255)                                                                ttACCCTTACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGGCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGc                                                                       <  2:231143/140‑1 (MQ=255)                                                                 tACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGGCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCt                                                                      <  2:389418/140‑1 (MQ=255)                                                                  aCCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGGCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCt                                                                      <  2:554410/139‑1 (MQ=255)                                                                  aCCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGGCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCt                                                                      >  1:554410/1‑139 (MQ=255)                                                                      tGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGGCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGtt                                                                 <  2:573268/140‑1 (MQ=255)                                                                        aCCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGGCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTcc                                                               >  1:740249/1‑140 (MQ=255)                                                                        aCCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGGCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTcc                                                               >  1:855366/1‑140 (MQ=255)                                                                             cTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGGCGAGTCACCATGGGGATGGTATTTACCTATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCa                                                          <  2:410793/140‑1 (MQ=255)                                                                             cTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGGCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCa                                                          <  1:388984/140‑1 (MQ=255)                                                                             cTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGGCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCa                                                          <  2:142067/140‑1 (MQ=255)                                                                               aCCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGGCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCAtt                                                        <  2:152408/140‑1 (MQ=255)                                                                                    cATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGGCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTa                                                   >  1:99784/1‑140 (MQ=255)                                                                                     atatAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGGCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAg                                                  <  2:406130/140‑1 (MQ=255)                                                                                        taACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGGCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGc                                                      >  1:858652/1‑133 (MQ=255)                                                                                        taACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGGCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGc                                                      <  2:858652/133‑1 (MQ=255)                                                                                         aaCGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGGCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACg                                              >  1:447133/1‑140 (MQ=255)                                                                                          aCGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGGCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGt                                             >  2:764586/1‑140 (MQ=255)                                                                                            gCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGGCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTc                                           <  2:818211/140‑1 (MQ=255)                                                                                              aGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGGCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCAt                                         >  2:108585/1‑140 (MQ=255)                                                                                              aGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGGCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAAAGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCAt                                         >  1:81421/1‑140 (MQ=255)                                                                                                   gaATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGGCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCa                                          <  2:549369/134‑1 (MQ=255)                                                                                                   gaATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGGCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCa                                          >  1:549369/1‑134 (MQ=255)                                                                                                           gcgcCATGCGGACGATCGTGTCATAGACCGGCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTAcg                            <  2:447133/140‑1 (MQ=255)                                                                                                              ccATGCGGACGATCGTGTCATAGACCGGCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGa                         >  2:251166/1‑140 (MQ=255)                                                                                                              ccATGCGGACGATCGTGTCATAGACCGGCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGa                         <  2:855366/140‑1 (MQ=255)                                                                                                              ccATGCGGACGATCGTGTCATAGACCGGCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGa                         <  2:76720/140‑1 (MQ=255)                                                                                                              ccATGCGGACGATCGTGTCATAGACCGGCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGa                         <  2:740249/140‑1 (MQ=255)                                                                                                                 tGCGGACGATCGTGTCATAGACCGGCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGACgg                      >  2:358012/1‑140 (MQ=255)                                                                                                                 tGCGGACGATCGTGTCATAGACCGGCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGACgg                      >  1:269145/1‑140 (MQ=255)                                                                                                                     gACGATCGTGTCATAGACCGGCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGACGGTGTa                  <  2:393792/140‑1 (MQ=255)                                                                                                                       cGATCGTGTCATAGACCGGCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAAAGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGACGGTGTAcc                <  2:81421/140‑1 (MQ=255)                                                                                                                           cGTGTCATAGACCGGCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGACGGTGTACCGGCt            >  2:56124/1‑140 (MQ=255)                                                                                                                             tgtCATAGACCGGCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGACGGTGTACCGGCTTc          >  2:541048/1‑140 (MQ=255)                                                                                                                                cATAGACCGGCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGACg                       <  2:861902/124‑1 (MQ=255)                                                                                                                                cATAGACCGGCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGACg                       >  1:861902/1‑124 (MQ=255)                                                                                                                                cATAGACCGGCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGACGGTGTACCGGCTTCAgg       <  2:92359/140‑1 (MQ=255)                                                                                                                                   aGACCGGCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGACGGTGTACCGGCTTCAGGCCa    <  1:764586/140‑1 (MQ=255)                                                                                                                                    gACCGGCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGACGGTGTACCGGCTTCAGGCCAt   >  1:373426/1‑140 (MQ=255)                                                                                                                                    gACCGGCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGACGGTGTACCGGCTTCAGGCCAt   >  1:772995/1‑140 (MQ=255)                                                                                                                                     aCCGGCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGACGGTGTACCGGCTTCAGGCCAtc  >  1:898621/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                        TTCGCCAGACGGATTTCCGTATAACGCATTGCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGCCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGACGGTGTACCGGCTTCAGGCCATC  >  NC_000913/2339035‑2339306

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑