Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,906,299	G→T	G197C (GGC→TGC)	ygcG →	protein YgcG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,906,299	0	G	T	98.3%	192.0 / ‑2.7	59	G197C (GGC→TGC)	ygcG	protein YgcG
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base T (31/27);  total (32/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.93e-01

AACACCAATATCCGACTAATCCTTCAGAAAGTGAATCAGCGTCTTCCAGTGATCATTATTACTTTGCTATTTTTTGGGTATTTGCAGTGATGTTCTTCCCTTTCTGGTTTTTTCATCAAGGCAGTAATTTTTGTCGCGCATGTAAAAGTGGAGTCTGTATTTCAGCAATCTATCTTTTAGATTTATTCCTGTTCTCCGATAAAATTTTTTCCATTGCTGTATTTTCCTTCTTTTTTACTTTCACCATATTTATG  >  NC_000913/2906180‑2906433                                                                                                                        |                                                                                                                                       aacaCCAATATCCGACTAATCCTTCAGAAAGTGAATCAGCGTCTTCCAGTGATCATTATTACTTTGCTATTTTTTGGGTATTTGCAGTGATGTTCTTCCCTTTCTGGTTTTTTCATCAATGCAGTAATTTTTGTCGCGCa                                                                                                                    >  1:746057/1‑140 (MQ=255) aacaCCAATATCCGACTAATCCTTCAGAAAGTGAATCAGCGTCTTCCAGTGATCATTATTACTTTGCTATTTTTTGGGTATTTGCAGTGATGTTCTTCCCTTTCTGGTTTTTTCATCAATGCAGTAATTTTTGTCGCGCa                                                                                                                    <  1:577963/140‑1 (MQ=255)     ccAATATCCGACTAATCCTTCAGAAAGTGAATCAGCGTCTTCCAGTGATCATTATTACTTTGCTATTTTTTGGGTATTTGCAGTGATGTTCTTCCCTTTCTGGTTTTTTCATCAATGCAGTAATTTTTGTCGCGCATGTa                                                                                                                >  2:330204/1‑140 (MQ=255)     ccAATATCCGACTAATCCTTCAGAAAGTGAATCAGCGTCTTCCAGTGATCATTATTACTTTGCTATTTTTTGGGTATTTGCAGTGATGTTCTTCCCTTTCTGGTTTTTTCATCAATGCAGTAATTTTTGTCGCGCATGTa                                                                                                                <  2:771773/140‑1 (MQ=255)         tatCCGACTAATCCTTCAGAAAGTGAATCAGCGTCTTCCAGTGATCATTATTACTTTGCTATTTTTTGGGTATTTGCAGTGATGTTCTTCCCTTTCTGGTTTTTTCATCAATGCAGTAATTTTTGTCGCGCATGTAAAAg                                                                                                            >  1:214324/1‑140 (MQ=255)            ccGACTAATCCTTCAGAAAGTGAATCAGCGTCTTCCAGTGATCATTATTACTTTGCTATTTTTTGGGTATTTGCAGTGATGTTCTTCCCTTTCTGGTTTTTTCATCAATGCAGTAATTTTTGTCGCGCATGTAAAAGTgg                                                                                                         >  1:592511/1‑140 (MQ=255)                cTAATCCTTCAGAAAGTGAATCAGCGTCTTCCAGTGATCATTATTACTTTGCTATTTTTTGGGTATTTGCAGTGATGTTCTTCCCTTTCTGGTTTTTTCATCAATGCAGTAATTTTTGTCGCGCATGTAAAAGTGGAGTc                                                                                                     >  2:770152/1‑140 (MQ=255)                cTAATCCTTCAGAAAGTGAATCAGCGTCTTCCAGTGATCATTATTACTTTGCTATTTTTTGGGTATTTGCAGTGATGTTCTTCCCTTTCTGGTTTTTTCATCAATGCAGTAATTTTTGTCGCGCATGTAAAAGTGGAGTc                                                                                                     <  2:768673/140‑1 (MQ=255)                cTAATCCTTCAGAAAGTGAATCAGCGTCTTCCAGTGATCATTATTACTTTGCTATTTTTTGGGTATTTGCAGTGATGTTCTTCCCTTTCTGGTTTTTTCATCAATGCAGTAATTTTTGTCGCGCATGTAAAAGTGGAGTc                                                                                                     <  2:387435/140‑1 (MQ=255)                cTAATCCTTCAGAAAGTGAATCAGCGTCTTCCAGTGATCATTATTACTTTGCTATTTTTTGGGTATTTGCAGTGATGTTCTTCCCTTTCTGGTTTTTTCATCAATGCAGTAATTTTTGTCGCGCATGTAAAAGTGGAGTc                                                                                                     >  1:90416/1‑140 (MQ=255)                   aTCCTTCAGAAAGTGAATCAGCGTCTTCCAGTGATCATTATTACTTTGCTATTTTTTGGGTATTTGCAGTGATGTTCTTCCCTTTCTGGTTTTTTCATCAATGCAGTAATTTTTGTCGCGCATGTAAAAGTg                                                                                                          >  1:337141/1‑132 (MQ=255)                   aTCCTTCAGAAAGTGAATCAGCGTCTTCCAGTGATCATTATTACTTTGCTATTTTTTGGGTATTTGCAGTGATGTTCTTCCCTTTCTGGTTTTTTCATCAATGCAGTAATTTTTGTCGCGCATGTAAAAGTg                                                                                                          <  2:337141/132‑1 (MQ=255)                   aTCCTTCAGAAAGTGAATCAGCGTCTTCCAGTGATCATTATTACTTTGCTATTTTTTGGGTATTTGCAGTGATGTTCTTCCCTTTCTGGTTTTTTCATCAATGCAGTAATTTTTGTCGCGCATGTAAAAGTGGAGTCTGt                                                                                                  >  2:244655/1‑140 (MQ=255)                              aGTGAATCAGCGTCTTCCAGTGATCATTATTACTTTGCTATTTTTTGGGTATTTGCAGTGATGTTCTTCCCTTTCTGGTTTTTTCATCAATGCAGTAATTTTTGTCGCGCATGTAAAAGTGGAGTCTGTATTTCa                                                                                            >  2:12330/1‑135 (MQ=255)                              aGTGAATCAGCGTCTTCCAGTGATCATTATTACTTTGCTATTTTTTGGGTATTTGCAGTGATGTTCTTCCCTTTCTGGTTTTTTCATCAATGCAGTAATTTTTGTCGCGCATGTAAAAGTGGAGTCTGTATTTCa                                                                                            <  1:12330/135‑1 (MQ=255)                              aGTGAATCAGCGTCTTCCAGTGATCATTATTACTTTGCTATTTTTTGGGTATTTGCAGTGATGTTCTTCCCTTTCTGGTTTTTTCATCAATGCAGTAATTTTTGTCGCGCATGTAAAAGTGGAGTCTGTATTTCAGCAat                                                                                       >  1:127999/1‑140 (MQ=255)                                       gCGTCTTCCAGTGATCATTATTACTTTGCTATTTTTTGGGTATTTGCAGTGATGTTCTTCCCTTTCTGGTTTTTTCATCAATGCAGTAATTTTTGTCGCGCATGTAAAAGTGGAGTCTGTATTTCAGCAATCTATCtttt                                                                              <  1:536996/140‑1 (MQ=255)                                            ttCCAGTGATCATTATTACTTTGCTATTTTTTGGGTATTTGCAGTGATGTTCTTCCCTTTCTGGTTTTTTCATCAATGCAGTAATTTTTGTCGCGCATGTAAAAGTGGAGTCTGTATTTCAGCAATCTATCTTTTAGAtt                                                                         <  1:330204/140‑1 (MQ=255)                                                      cATTATTACTTTGCTATTTTTTGGGTATTTGCAGTGATGTTCTTCCCTTTCTGGTTTTTTCATCAATGCAGTAATTTTTGTCGCGCATGTAAAAGTGGAGTCTGTATTTCAGCAATCTATCTTTTAGATTTATTCCTGtt                                                               >  2:73056/1‑140 (MQ=255)                                                         tattaCTTTGCTATTTTTTGGGTATTTGCAGTGATGTTCTTCCCTTTCTGGTTTTTTCATCAATGCAGTAATTTTTGTCGCGCATGTAAAAGTGGAGTCTGTATTTCAGCAATCTATCTTTTAGATTTATTCCTGTtctc                                                            <  2:90416/140‑1 (MQ=255)                                                         tattaCTTTGCTATTTTTTGGGTATTTGCAGTGATGTTCTTCCCTTTCTGGTTTTTTCATCAATGCAGTAATTTTTGTCGCGCATGTAAAAGTGGAGTCTGTATTTCAGCAATCTATCTTTTAGATTTATTCCTGTtctc                                                            >  2:483122/1‑140 (MQ=255)                                                           ttaCTTTGCTATTTTTTGGGTATTTGCAGTGATGTTCTTCCCTTTCTGGTTTTTTCATCAATGCAGTAATTTTTGTCGCGCATGTAAAAGTGGAGTCTGTATTTCAGCAATCTATCTTTTAGATTTATTCCTGTTCTCCg                                                          >  1:835724/1‑140 (MQ=255)                                                                ttGCTATTTTTTGGGTATTTGCAGTGATGTTCTTCCCTTTCTGGTTTTTTCATCAATGCAGTAATTTTTGTCGCGCATGTAAAAGTGGAGTCTGTATTTCAGCAATCTATCTTTTAGATTTATTCCTGTTCTCCGATaaa                                                     >  2:840613/1‑140 (MQ=255)                                                                 tGCTATTTTTTGGGTATTTGCAGTGATGTTCTTCCCTTTCTGGTTTTTTCATCAATGCAGTAATTTTTGTCGCGCATGTAAAAGTGGAGTCTGTATTTCAGCAATCTATCTTTTAGATTTATTCCTGTTCTCCGATaaaa                                                    >  2:87070/1‑140 (MQ=255)                                                                 tGCTATTTTTTGGGTATTTGCAGTGATGTTCTTCCCTTTCTGGTTTTTTCATCAATGCAGTAATTTTTGTCGCGCATGTAAAAGTGGAGTCTGTATTTCAGCAATCTATCTTTTAGATTTATTCCTGTTCTCCGATaaaa                                                    >  1:106888/1‑140 (MQ=255)                                                                 tGCTATTTTTTGGGTATTTGCAGTGATGTTCTTCCCTTTCTGGTTTTTTCATCAATGCAGTAATTTTTGTCGCGCATGTAAAAGTGGAGTCTGTATTTCAGCAATCTATCTTTTAGATTTATTCCTGTTCTCCGATaaaa                                                    >  1:367893/1‑140 (MQ=255)                                                                   cTATTTTTTGGGTATTTGCAGTGATGTTCTTCCCTTTCTGGTTTTTTCATCAATGCAGTAATTTTTGTCGCGCATGTAAAAGTGGAGTCTGTATTTCAGCAATCTATCTTTTAGATTTATTCCTGTTCTCCGATAAAAtt                                                  >  1:730433/1‑140 (MQ=255)                                                                   cTATTTTTTGGGTATTTGCAGTGATGTTCTTCCCTTTCTGGTTTTTTCATCAATGCAGTAATTTTTGTCGCGCATGTAAAAGTGGAGTCTGTATTTCAGCAATCTATCTTTTAGATTTATTCCTGTTCTCCGATAAAAtt                                                  >  2:762401/1‑140 (MQ=255)                                                                          ttGGGTATTTGCAGTGATGTTCTTCCCTTTCTGGTTTTTTCATCAATGCAGTAATTTTTGTCGCGCATGTAAAAGTGGAGTCTGTATTTCAGCAATCTATCTTTTAGATTTATTCCTGTTCTCCGATAAAATTTTTTCCa                                           <  1:483122/140‑1 (MQ=255)                                                                          ttGGGTATTTGCAGTGATGTTCTTCCCTTTCTGGTTTTTTCATCAATGCAGTAATTTTTGTCGCGCATGTAAAAGTGGAGTCTGTATTTCAGCAATCTATCTTTTAGATTTATTCCTGTTCTCCGATAAAATTTTTTCCa                                           <  2:367893/140‑1 (MQ=255)                                                                           tGGGTATTTGCAGTGATGTTCTTCCCTTTCTGGTTTTTTCATCAATGCAGTAATTTTTGTCGCGCATGTAAAAGTGGAGTCTGTATTTCAGCAATCTATCTTTTAGATTTATTCCTGTTCTCCGATAAAAtttt                                                <  2:575634/134‑1 (MQ=255)                                                                           tGGGTATTTGCAGTGATGTTCTTCCCTTTCTGGTTTTTTCATCAATGCAGTAATTTTTGTCGCGCATGTAAAAGTGGAGTCTGTATTTCAGCAATCTATCTTTTAGATTTATTCCTGTTCTCCGATAAAAtttt                                                >  1:575634/1‑134 (MQ=255)                                                                           tGGGTATTTGCAGTGATGTTCTTCCCTTTCTGGTTTTTTCATCAATGCAGTAATTTTTGTCGCGCATGTAAAAGTGGAGTCTGTATTTCAGCAATCTATCTTTTAGATTTATTCCTGTTCTCCGATAAAATTTTTTCCAt                                          >  1:566358/1‑140 (MQ=255)                                                                             ggTATTTGCAGTGATGTTCTTCCCTTTCTGGTTTTTTCATCAATGCAGTAATTTTTGTCGCGCATGTAAAAGTGGAGTCTGTATTTCAGCAATCTATCTTTTAGATTTATTCCTGTTCTCCGATAAAATTTTTTCCATTg                                        >  1:629985/1‑140 (MQ=255)                                                                                  ttGCAGTGATGTTCTTCCCTTTCTGGTTTTTTCATCAATGCAGTAATTTTTGTCGCGCATGTAAAAGTGGAGTCTGTATTTCAGCAATCTATCTTTTAGATTTATTCCTGTTCTCCGATAAAATTTTTTCCATTGCTGTa                                   >  1:186578/1‑140 (MQ=255)                                                                                        tGATGTTCTTCCCTTTCTGGTTTTTTCATCAATGCAGTAATTTTTGTCGCGCATGTAAAAGTGGAGTCTGTATTTCAGCAATCTATCTTTTAGATTTATTCCTGTTCTCCGATAAAATTTTTTCCATTGCTGTATTTTcc                             <  1:762401/140‑1 (MQ=255)                                                                                         gATGTTCTTCCCTTTCTGGTTTTTTCATCAATGCAGTAATTTTTGTCGCGCATGTAAAAGTGGAGTCTGTATTTCAGCAATCTATCTTTTAGATTTATTCCTGTTCTCCGATAAAATTTTTTCCATTGCTGTATTTTCct                            <  2:901553/140‑1 (MQ=255)                                                                                           tGTTCTTCCCTTTCTGGTTTTTTCATCAAGGCAGTAATTTTTGTCGCGCATGTAAAAGTGGAGTCTGTATTTCAGCAATCTATCTTTTAGATTTATTCCTGTTCTCCGATAAAATTTTTTCCATTGCTGTATTTTCcttc                          >  2:726675/1‑140 (MQ=255)                                                                                               cttcCCTTTCTGGTTTTTTCATCAATGCAGTAATTTTTGTCGCGCATGTAAAAGTGGAGTCTGTATTTCAGCAATCTATCTTTTAGATTTATTCCTGTTCTCCGATAAAATTTTTTCCATTGCTGTATTTTCCTTCtttt                      >  1:111086/1‑140 (MQ=255)                                                                                                  cccTTTCTGGTTTTTTCATCAATGCAGTAATTTTTGTCGCGCATGTAAAAGTGGAGTCTGTATTTCAGCAATCTATCTTTTAGATTTATTCCTGTTCTCCGATAAAATTTTTTCCATTGCTGTATTTTCCTTCTTTTTTa                   >  1:865041/1‑140 (MQ=255)                                                                                                  cccTTTCTGGTTTTTTCATCAATGCAGTAATTTTTGTCGCGCATGTAAAAGTGGAGTCTGTATTTCAGCAATCTATCTTTTAGATTTATTCCTGTTCTCCGATAAAATTTTTTCCATTGCTGTATTTTCCTTCTTTTTTa                   >  2:559739/1‑140 (MQ=255)                                                                                                     tttCTGGTTTTTTCATCAATGCAGTAATTTTTGTCGCGCATGTAAAAGTGGAGTCTGTATTTCAGCAATCTATCTTTTAGATTTATTCCTGTTCTCCGATAAAATTTTTTCCATTGCTGTATTTTCCTTCTTTTTTACtt                >  1:212045/1‑140 (MQ=255)                                                                                                     tttCTGGTTTTTTCATCAATGCAGTAATTTTTGTCGCGCATGTAAAAGTGGAGTCTGTATTTCAGCAATCTATCTTTTAGATTTATTCCTGTTCTCCGATAAAATTTTTTCCATTGCTGTATTTTCCTTCTTTTTTACtt                >  1:144229/1‑140 (MQ=255)                                                                                                      ttCTGGTTTTTTCATCAATGCAGTAATTTTTGTCGCGCATGTAAAAGTGGAGTCTGTATTTCAGCAATCTATCTTTTAGATTTATTCCTGTTCTCCGATAAAATTTTTTCCATTGCTGTATTTTCCTTCTTTTTTACttt               <  2:726057/140‑1 (MQ=255)                                                                                                      ttCTGGTTTTTTCATCAATGCAGTAATTTTTGTCGCGCATGTAAAAGTGGAGTCTGTATTTCAGCAATCTATCTTTTAGATTTATTCCTGTTCTCCGATAAAATTTTTTCCATTGCTGTATTTTCCTTCTTTTTTACttt               <  1:840613/140‑1 (MQ=255)                                                                                                      ttCTGGTTTTTTCATCAATGCAGTAATTTTTGTCGCGCATGTAAAAGTGGAGTCTGTATTTCAGCAATCTATCTTTTAGATTTATTCCTGTTCTCCGATAAAATTTTTTCCATTGCTGTATTTTCCTTCTTTTTTACttt               <  1:559739/140‑1 (MQ=255)                                                                                                       tCTGGTTTTTTCATCAATGCAGTAATTTTTGTCGCGCATGTAAAAGTGGAGTCTGTATTTCAGCAATCTATCTTTTAGATTTATTCCTGTTCTCCGATAAAATTTTTTCCATTGCTGTATTTTCCTTCTTTTTTACTTTc              >  2:100901/1‑140 (MQ=255)                                                                                                       tCTGGTTTTTTCATCAATGCAGTAATTTTTGTCGCGCATGTAAAAGTGGAGTCTGTATTTCAGCAATCTATCTTTTAGATTTATTCCTGTTCTCCGATAAAATTTTTTCCATTGCTGTATTTTCCTTCTTTTTTACTTTc              >  1:940883/1‑140 (MQ=255)                                                                                                        cTGGTTTTTTCATCAATGCAGTAATTTTTGTCGCGCATGTAAAAGTGGAGTCTGTATTTCAGCAATCTATCTTTTAGATTTATTCCTGTTCTCCGATAAAATTTTTTCCATTGCTGTATTTTCCTTCTTTTTTACTTTCa             >  2:268161/1‑140 (MQ=255)                                                                                                         tGGTTTTTTTATCAATGCAGTAATTTTTGTCGCGCATGTAAAAGTGGAGTCTGTATTTCAGCAATCTATCTTTTAGATTTATTCCTGTTCTCCGATAAAATTTTTTCCATTGCTGTATTTTCCTTCTTTTTTACTTTCAc            <  1:725569/140‑1 (MQ=255)                                                                                                         tGGTTTTTTCATCAATGCAGTAATTTTTGTCGCGCATGTAAAAGTGGAGTCTGTATTTCAGCAATCTATCTTTTAGATTTATTCCTGTTCTCCGATAAAATTTTTTCCATTGCTGTATTTTCCTTCTTTTTTACTTTCAc            <  2:52106/140‑1 (MQ=255)                                                                                                          ggTTTTTTCATCAATGCAGTAATTTTTGTCGCGCATGTAAAAGTGGAGTCTGTATTTCAGCAATCTATCTTTTAGATTTATTCCTGTTCTCCGATAAAATTTTTTCCATTGCTGTATTTTCCTTCTTTTTTACTTTCAcc           <  2:940883/140‑1 (MQ=255)                                                                                                           gTTTTTTCATCAATGCAGTAATTTTTGTCGCGCATGTAAAAGTGGAGTCTGTATTTCAGCAATCTATCTTTTAGATTTATTCCTGTTCTCCGATAAAATTTTTTCCATTGCTGTATTTTCCTTCTTTTTTACTTTCACCa          <  2:566358/140‑1 (MQ=255)                                                                                                           gTTTTTTCATCAATGCAGTAATTTTTGTCGCGCATGTAAAAGTGGAGTCTGTATTTCAGCAATCTATCTTTTAGATTTATTCCTGTTCTCCGATAAAATTTTTTCCATTGCTGTATTTTCCTTCTTTTTTACTTTCACCa          <  2:730433/140‑1 (MQ=255)                                                                                                           gTTTTTTCATCAATGCAGTAATTTTTGTCGCGCATGTAAAAGTGGAGTCTGTATTTCAGCAATCTATCTTTTAGATTTATTCCTGTTCTCCGATAAAATTTTTTCCATTGCTGTATTTTCCTTCTTTTTTACTTTCACCa          <  1:100901/140‑1 (MQ=255)                                                                                                                 tcatcaATGCAGTAATTTTTGTCGCGCATGTAAAAGTGGAGTCTGTATTTCAGCAATCTATCTTTTAGATTTATTCCTGTTCTCCGATAAAATTTTTTCCATTGCTGTATTTTCCTTCTTTTTTACTTTCACCATATTTa    <  2:746057/140‑1 (MQ=255)                                                                                                                 tcatcaATGCAGTAATTTTTGTCGCGCATGTAAAAGTGGAGTCTGTATTTCAGCAATCTATCTTTTAGATTTATTCCTGTTCTCCGATAAAATTTTTTCCATTGCTGTATTTTCCTTCTTTTTTACTTTCACCATATTTa    <  2:144229/140‑1 (MQ=255)                                                                                                                 tcatcaATGCAGTAATTTTTGTCGCGCATGTAAAAGTGGAGTCTGTATTTCAGCAATCTATCTTTTAGATTTATTCCTGTTCTCCGATAAAATTTTTTCCATTGCTGTATTTTCCTTCTTTTTTACTTTCACCATATTTa    <  1:196297/140‑1 (MQ=255)                                                                                                                   atcaATGCAGTAATTTTTGTCGCGCATGTAAAAGTGGAGTCTGTATTTCAGCAATCTATCTTTTAGATTTATTCCTGTTCTCCGATAAAATTTTTTCCATTGCTGTATTTTCCTTCTTTTTTACTTTCACCATATTTATg  <  1:87070/140‑1 (MQ=255)                                                                                                                        |                                                                                                                                       AACACCAATATCCGACTAATCCTTCAGAAAGTGAATCAGCGTCTTCCAGTGATCATTATTACTTTGCTATTTTTTGGGTATTTGCAGTGATGTTCTTCCCTTTCTGGTTTTTTCATCAAGGCAGTAATTTTTGTCGCGCATGTAAAAGTGGAGTCTGTATTTCAGCAATCTATCTTTTAGATTTATTCCTGTTCTCCGATAAAATTTTTTCCATTGCTGTATTTTCCTTCTTTTTTACTTTCACCATATTTATG  >  NC_000913/2906180‑2906433

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑