Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,392,453	C→T	intergenic (+18/‑19)	glyV → / → glyX	tRNA‑Gly/tRNA‑Gly

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,392,453	0	C	T	100.0%	26.8 / NA	10	intergenic (+18/‑19)	glyV/glyX	tRNA‑Gly/tRNA‑Gly
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (4/6);  total (4/6)

CGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGT  >  NC_000913/4392319‑4392487                                                                                                                                       |                                   cgCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTG‑AAAGTGCTGTAAGGca                              >  1:491548/1‑140 (MQ=255) cgCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTG‑AAAGTGCTGTAAGGca                              >  1:901176/1‑140 (MQ=255) cgCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTG‑AAAGTGCTGTAAGGca                              >  2:141922/1‑140 (MQ=255) cgCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTG‑AAAGTGCTGTAAGGca                              >  2:329368/1‑140 (MQ=255)      aaaTGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTG‑AAAGTGCTGTAAGGCACAGac                         <  2:559959/140‑1 (MQ=255)          gCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTG‑AAAGTGCTGTAAGGCACAGaccacc                     <  2:317542/140‑1 (MQ=255)                  tACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTG‑AAAGTGCTGTAAGGCACAGACCACCCAAGCggg             <  2:143757/140‑1 (MQ=255)                        aGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTG‑AAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGc       <  1:141922/140‑1 (MQ=255)                         gCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTG‑AAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCt      <  1:329368/140‑1 (MQ=255)                             aaTTAAGTAGTACGCGGGAATAGCTCAGTGGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATGTG‑AAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGt  <  1:338816/140‑1 (MQ=11)                                                                                                                                       |                                   CGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGT  >  NC_000913/4392319‑4392487

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑