Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	312,327	A→T	R72R (CGA→CGT)	ykgL →	uncharacterized protein YkgL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	312,327	0	A	T	100.0%	173.5 / NA	51	R72R (CGA→CGT)	ykgL	uncharacterized protein YkgL
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (32/19);  total (32/19)

AAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTGGATTAGATTTACAAATCACATATAGCCGTCCTTTTCGCTTCACAATCTG  >  NC_000913/312191‑312463                                                                                                                                         |                                                                                                                                         aaGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGTTCt                                                                                                                                       <  1:233283/140‑1 (MQ=255)                   aCATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGTTCTGATTATTAATCAGACaaa                                                                                                                     <  2:272971/140‑1 (MQ=255)                   aCATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGTTCTGATTATTAATCAGACaaa                                                                                                                     <  2:482192/140‑1 (MQ=255)                   aCATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGTTCTGATTATTAATCAGACaaa                                                                                                                     <  1:674455/140‑1 (MQ=255)                              aTGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGTTCTGATTATTAATCAGACAAATATCGTTa                                                                                                             <  2:417613/137‑1 (MQ=255)                              aTGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGTTCTGATTATTAATCAGACAAATATCGTTa                                                                                                             >  1:417613/1‑137 (MQ=255)                                 gAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGTTCTGATTATTAATCAGACAAATATCGTTAATCCGt                                                                                                       <  1:733729/140‑1 (MQ=255)                                        cGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGTTCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAAttt                                                                                                <  2:529766/140‑1 (MQ=255)                                         ggAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGTTCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAAtttt                                                                                               >  2:654349/1‑140 (MQ=255)                                         ggAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGTTCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAAtttt                                                                                               >  1:517117/1‑140 (MQ=255)                                         ggAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGTTCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAAtttt                                                                                               >  1:197180/1‑140 (MQ=255)                                                            ttGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGTTCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTa                                                                            <  1:896808/140‑1 (MQ=255)                                                             tGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGTTCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTAc                                                                           >  2:771273/1‑140 (MQ=255)                                                                ccTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGTTCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGAc                                                                        <  1:772532/140‑1 (MQ=255)                                                                      aTGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGTTCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGaa                                                                  <  1:654349/140‑1 (MQ=255)                                                                         aaCAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGTTCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACgg                                                               >  1:225500/1‑140 (MQ=255)                                                                              gaagaaTATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGTTCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTa                                                          <  1:771273/140‑1 (MQ=255)                                                                                  aaTATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGTTCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAAc                                                      <  1:860386/140‑1 (MQ=255)                                                                                        tCCCCAGGAAAATTAATGTTGGTGTTATACACACACCAACATTAATCCGTTCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGATTTTTCTTACGACCCTGAACGGCCTCAAAACGTGGAt                                                >  2:699308/1‑140 (MQ=255)                                                                                        tCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGTTCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTGGAt                                                >  2:708918/1‑140 (MQ=255)                                                                                        tCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGTTCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTGGAt                                                >  2:275832/1‑140 (MQ=255)                                                                                            cAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGTTCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTGGATTAGa                                            >  2:252213/1‑140 (MQ=255)                                                                                            cAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGTTCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTGGATTAGa                                            >  1:776981/1‑140 (MQ=255)                                                                                              ggAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGTTCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTGGATTAGAtt                                          >  2:438566/1‑140 (MQ=255)                                                                                              ggAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGTTCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTGGATTAGAtt                                          >  2:359826/1‑140 (MQ=255)                                                                                                 aaaTTAATGTTGGTGTTATAAACACACCAACATTAATCCGTTCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTGGATTAGATTTAc                                       <  1:299515/140‑1 (MQ=255)                                                                                                  aaTTAATGTTGGTGTTATAAACACACCAACATTAATCCGTTCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTGGATTAGATTTACa                                      <  1:104344/140‑1 (MQ=255)                                                                                                    ttAATGTTGGTGTTATAAACACACCAACATTAATCCGTTCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTGGATTAGATTTACaaa                                    <  1:139224/140‑1 (MQ=255)                                                                                                                ttATAAACACACCAACATTAATCCGTTCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTGGATTAGATTTACAAATCACATATAGcc                        >  1:720841/1‑140 (MQ=255)                                                                                                                 tataAACACACCAACATTAATCCGTTCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTGGATTAGATTTACAAATCACATATAGCCg                       <  2:438224/140‑1 (MQ=255)                                                                                                                       cacacCAACATTAATCCGTTCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTGGATTAGATTTACAAATCACATATAGCCGTCCttt                 >  2:813068/1‑140 (MQ=255)                                                                                                                         cacCAACATTAATCCGTTCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTGGATTAGATTTACAAATCACATATAGCCGTCCTTTTc               >  2:783316/1‑140 (MQ=255)                                                                                                                          atcAACATTAATCCGTTCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTGGATTAGATTTACAAATCACATATAGCCGTCCTTTTCg              >  2:156111/3‑140 (MQ=255)                                                                                                                          acCAACATTAATCCGTTCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTGGATTAGATTTACAAATCACATATAGCCGTCCTTTTCg              <  2:517117/140‑1 (MQ=255)                                                                                                                          acCAACATTAATCCGTTCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTGGATTAGATTTACAAATCACATATAGCCGTCCTTTTCg              >  2:294555/1‑140 (MQ=255)                                                                                                                          acCAACATTAATCCGTTCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTGGATTAGATTTACAAATCACATATAGCCGTCCTTTTCg              >  2:134278/1‑140 (MQ=255)                                                                                                                          acCAACATTAATCCGTTCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTGGATTAGATTTACAAATCACATATAGCCGTCCTTTTCg              >  2:247864/1‑140 (MQ=255)                                                                                                                          acCAACATTAATCCGTTCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTGGATTAGATTTACAAATCACATATAGCCGTCCTTTTCg              <  2:197180/140‑1 (MQ=255)                                                                                                                          acCAACATTAATCCGTTCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTGGATTAGATTTACAAATCACATATAGCCGTCCTTTTCg              >  2:261106/1‑140 (MQ=255)                                                                                                                          acCAACATTAATCCGTTCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTGGATTAGATTTACAAATCACATATAGCCGTCCTTTTCg              >  1:745582/1‑140 (MQ=255)                                                                                                                          acCAACATTAATCCGTTCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTGGATTAGATTTACAAATCACATATAGCCGTCCTTTTCg              >  1:239845/1‑140 (MQ=255)                                                                                                                          acCAACATTAATCCGTTCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTGGATTAGATTTACAAATCACATATAGCCGTCCTTTTCg              >  1:297768/1‑140 (MQ=255)                                                                                                                            cAACATTAATCCGTTCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTGGATTAGATTTACAAATCACATATAGCCGTCCTTTTCGCt            >  2:300883/1‑140 (MQ=255)                                                                                                                            cAACATTAATCCGTTCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTGGATTAGATTTACAAATCACATATAGCCGTCCTTTTCGCt            >  2:71505/1‑140 (MQ=255)                                                                                                                             aaCATTAATCCGTTCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTGGATTAGATTTACAAATCACATATAGCCGTCTTTTTCGCtt           >  1:48473/1‑140 (MQ=255)                                                                                                                                 ttAATCCGTTCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTGGATTAGATTTACAAATCACATATAGCCGTCCTTTTCGCTTcaca       <  1:438566/140‑1 (MQ=255)                                                                                                                                  tAATCCGTTCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTGGATTAGATTTACAAATCACATATAGCCGTCCTTTTCGCTTCACaa      >  2:192747/1‑140 (MQ=255)                                                                                                                                    aTCCGTTCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTGGATTAGATTTACAAATCACATATAGCCGTCCTTTTCGCtt           >  2:160730/1‑133 (MQ=255)                                                                                                                                    aTCCGTTCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTGGATTAGATTTACAAATCACATATAGCCGTCCTTTTCGCtt           <  1:160730/133‑1 (MQ=255)                                                                                                                                    aTCCGTTCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTGGATTAGATTTACAAATCACATATAGCCGTCCTTTTCGCTTCACAATc    >  2:97557/1‑140 (MQ=255)                                                                                                                                    aTCCGTTCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTGGATTAGATTTACAAATCACATATAGCCGTCCTTTTCGCTTCACAATc    >  1:413039/1‑140 (MQ=255)                                                                                                                                    aTCCGTTCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTGGATTAGATTTACAAATCACATATAGCCGTCCTTTTCGCTTCACAATc    >  1:914767/1‑140 (MQ=255)                                                                                                                                    aTCCGTTCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTCAAAACGTGGATTAGATTTACAAATCACATATAGCCGTCCTTTTCGCTTCACAATc    >  1:479507/1‑140 (MQ=255)                                                                                                                                      ccGTTCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTGGATTAGATTTACAAATCACATATAGCCGTCCTTTTCGCTTCACAATCTg  <  1:156111/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                         AAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTGGATTAGATTTACAAATCACATATAGCCGTCCTTTTCGCTTCACAATCTG  >  NC_000913/312191‑312463

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑