Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,496,732	C→T	intergenic (+243/‑18)	leuX → / → intB	tRNA‑Leu/KpLE2 phage‑like element; putative integrase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,496,732	0	C	T	100.0%	278.7 / NA	82	intergenic (+243/‑18)	leuX/intB	tRNA‑Leu/KpLE2 phage‑like element; putative integrase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (41/41);  total (41/41)

TACATCTACCAACATATGTTGGTACAGATGATGGTATTTCCGGTTCGATAATGCTTGTACCAACAGGGAGGGAATACGATGGCATTAACAGATATCAAAGTCAGAGCAGCCAAGCCAACGGATAAGCAATATTAGCTGACTGATGGTGGCGGTATGCATCTGCTTGTCCATCCAAATGGTTCTAAGTACTGGCGTTTGCA‑GTACCGTTATGAGGGAAAGCAAAAAATGCTGGCACTTGGGGTTTATCCTGAAATCACACTAGCGGATGCCA  >  NC_000913/4496597‑4496867                                                                                                                                        |                                                                                                                                         tACATCTACCAACATATGTTGGTACAGATGATGGTATTTCCGGTTCGATAATGCTTGTACCAACAGGGAGGGAATACGATGGCATTAACAGATATCAAAGTCAGAGCAGCCAAGCCAACGGATAAGCAATATTAGttgac                                                                                                                                      >  2:36133/1‑140 (MQ=255)        aCCAACATATGTTGGTACAGATGATGGTATTTCTGGTTCGATAATGCTTGTACCAACAGGGAGGGAATACGATGGCATTAACAGATATCAAAGTCAGAGCAGCCAAGCCAACGGATAAGCAATATTAGTTGACTGAtggt                                                                                                                               >  2:265259/1‑140 (MQ=255)        aCCAACATATGTTGGTACAGATGATGGTATTTCCGGTTCGATAATGCTTGTACCAACAGGGAGGGAATACGATGGCATTAACAGATATCAAAGTCAGAGCAGCCAAGCCAACGGATAAGCAATATTAGTTGACTGAtggt                                                                                                                               <  2:786368/140‑1 (MQ=255)        aCCAACATATGTTGGTACAGATGATGGTATTTCCGGTTCGATAATGCTTGTACCAACAGGGAGGGAATACGAAGGCATTAACAGATATCAAAGTCAGAGCAGCCAAGCCAACGGATAAGCAATATTAGTTGACTGAtggt                                                                                                                               >  1:916232/1‑140 (MQ=255)         ccAACATATGTTGGTACAGATGATGGTATTTCCGGTTCGATAATGCTTGTACCAACAGGGAGGGAATACGATGGCATTAACAGATATCAAAGTCAGAGCAGCCAAGCCAACGGATAAGCAATATTAGTTGACTGAtggtg                                                                                                                              >  2:325210/1‑140 (MQ=255)         ccAACATATGTTGGTACAGATGATGGTATTTCCGGTTCGATAATGCTTGTACCAACAGGGAGGGAATACGATGGCATTAACAGATATCAAAGTCAGAGCAGCCAAGCCAACGGATAAACAATATTAGTTGACTGAtggtg                                                                                                                              >  1:259108/1‑140 (MQ=255)          cAACATATGTTGGTACAGATGATGGTATTTCCGGTTCGATAATGCTTGTACCAACAGGGAGGGAATACGATGGCATTAACAGATATCAAAGTCAGAGCAGCCAAGCCAACGGATAAGCAATATTAGTTGACTGAtggtgg                                                                                                                             <  2:632992/140‑1 (MQ=255)              atatGTTGGTACAGATGATGGTATTTCCGGTTCGATAATGCTTGTACCAACAGGGAGGGAATACGATGGCATTAACAGATATCAAAGTCAGAGCAGCCAAGCCAACGGATAAGCAATATTAGTTGACTGATGGTGGCGGt                                                                                                                         >  2:807033/1‑140 (MQ=255)               tatGTTGGTACAGATGATGGTATTTCCGGTTCGATAATGCTTGTACCAACAGGGAGGGAATACGATGGCATTAACAGATATCAAAGTCAGAGCAGCCAAGCCAACGGATAAGCAATATTAGTTGACTGATGGTGGCGGTa                                                                                                                        >  2:43476/1‑140 (MQ=255)                atGTTGGTACAGATGATGGTATTTCCGGTTCGATAATGCTTGTACCAACAGGGAGGGAATACGATGGCATTAACAGATATCAAAGTCAGAGCAGCCAAGCCAACGGATAAGCAATATTAGTTGACTGAtggtgg                                                                                                                             <  2:27578/134‑1 (MQ=255)                atGTTGGTACAGATGATGGTATTTCCGGTTCGATAATGCTTGTACCAACAGGGAGGGAATACGATGGCATTAACAGATATCAAAGTCAGAGCAGCCAAGCCAACGGATAAGCAATATTAGTTGACTGAtggtgg                                                                                                                             >  1:27578/1‑134 (MQ=255)                 tGTTGGTACAGATGATGGTATTTCCGGTTCGATAATGCTTGTACCAACAGGGAGGGAATACGATGGCATTAACAGATATCAAAGTCAGAGCAGCCAAGCCAACGGATAAGCAATATTAGTTGACTGAtgg                                                                                                                                >  1:918879/1‑130 (MQ=255)                 tGTTGGTACAGATGATGGTATTTCCGGTTCGATAATGCTTGTACCAACAGGGAGGGAATACGATGGCATTAACAGATATCAAAGTCAGAGCAGCCAAGCCAACGGATAAGCAATATTAGTTGACTGAtgg                                                                                                                                <  2:918879/130‑1 (MQ=255)                  gTTGGTACAGATGATGGTATTTCCGGTTCGATAATGCTTGTACCAACAGGGAGGGAATACGATGGCATTAACAGATATCAAAGTCAGAGCAGCCAAGCCAACGGATAAGCAATATTAGTTGACTGATGGTGGCGGTATGc                                                                                                                     <  1:43476/140‑1 (MQ=255)                   ttGGTACAGATGATGGTATTTCCGGTTCGATAATGCTTGTACCAACAGGGAGGGAATACGATGGCATTAACAGATATCAAAGTCAGAGCAGCCAAGCCAACGGATAAGCAATATTAGTTGACTGATGGTGGCGGTATGCa                                                                                                                    <  2:284101/140‑1 (MQ=255)                    tGGTACAGATGATGGTATTTCCGGTTCGATAATGCTTGTACCAACAGGGAGGGAATACGATGGCATTAACAGATATCAAAGTCAGAGCAGCCAAGCCAACGGATAAGCAATATTAGTTGACTGATGGTGGCGGTATGCAt                                                                                                                   >  1:892893/1‑140 (MQ=255)                      gTACAGATGATGGTATTTCCGGTTCGATAATGCTTGTACCAACAGGGAGGGAATACGATGGCATTAACAGATATCAAAGTCAGAGCAGCCAAGCCAACGGATAAGCAATATTAGTTGACTGATGGTGGCGGTATGCATCt                                                                                                                 >  1:212279/1‑140 (MQ=255)                       tACAGATGATGGTATTTCCGGTTCGATAATGCTTGTACCAACAGGGAGGGAATACGATGGCATTAACAGATATCAAAGTCAGAGCAGCCAAGCCAACGGATAAGCAATATTAGTTGACTGATGGTGGCGGTATGCATCTg                                                                                                                >  2:279144/1‑140 (MQ=255)                       tACAGATGATGGTATTTCCGGTTCGATAATGCTTGTACCAACAGGGAGGGAATACGATGGCATTAACAGATATCAAAGTCAGAGCAGCCAAGCCAACGGATAAGCAATATTAGTTGACTGATGGTGGCGGTATGCATCTg                                                                                                                >  2:70520/1‑140 (MQ=255)                        aCAGATGATGGTATTTCCGGTTCGATAATGCTTGTACCAACAGGGAGGGAATACGATGGCATTAACAGATATCAAAGTCAGAGCAGCCAAGCCAACGGATAAGCAATATTAGTTGACTGATGGTGGCGGTATGCATCTGc                                                                                                               >  2:875804/1‑140 (MQ=255)                        aCAGATGATGGTATTTCCGGTTCGATAATGCTTGTACCAACAGGGAGGGAATACGATGGCATTAACAGATATCAAAGTCAGAGCAGCCAAGCCAACGGATAAGCAATATTAGTTGACTGATGGTGGCGGTATGCATCTGc                                                                                                               >  1:649062/1‑140 (MQ=255)                         cAGATGATGGTATTTCCGGTTCGATAATGCTTGTACCAACAGGGAGGGAATACGATGGCATTAACAGATATCAAAGTCAGAGCAGCCAAGCCAACGGATAAGCAATATTAGTTGACTGATGGTGGCGGTATGCATCTGCt                                                                                                              >  1:797765/1‑140 (MQ=255)                            atgatgGTATTTCCGGTTCGATAATGCTTGTACCAACAGGGAGGGAATACGATGGCATTAACAGATATCAAAGTCAGAGCAGCCAAGCCAACGGATAAGCAATATTAGTTGACTGAtggtgg                                                                                                                             <  1:478990/122‑1 (MQ=255)                            atgatgGTATTTCCGGTTCGATAATGCTTGTACCAACAGGGAGGGAATACGATGGCATTAACAGATATCAAAGTCAGAGCAGCCAAGCCAACGGATAAGCAATATTAGTTGACTGAtggtgg                                                                                                                             >  2:478990/1‑122 (MQ=255)                            atgatgGTATTTCCGGTTCGATAATGCTTGTACCAACAGGGAGGGAATACGATGGCATTAACAGATATCAAAGTCAGAGCAGCCAAGCCAACGGATAAGCAATATTAGTTGACTGATGGTGGCGGTATGCATCTGCTTGt                                                                                                           >  2:874140/1‑140 (MQ=255)                            atgatgGTATTTCCGGTTCGATAATGCTTGTACCAACAGGGAGGGAATACGATGGCATTAACAGATATCAAAGTCAGAGCAGCCAAGCCAACGGATAAGCAATATTAGTTGACTGATGGTGGCGGTATGCATCTGCTTGt                                                                                                           <  2:212279/140‑1 (MQ=255)                             tgatgGTATTTCCGGTTCGATAATGCTTGTACCAACAGGGAGGGAATACGATGGCATTAACAGATATCAAAGTCAGAGCAGCCAAGCCAACGGATAAGCAATATTAGTTGACTGATGGTGGCGGTATGCATCTGCTTGtc                                                                                                          <  1:279144/140‑1 (MQ=255)                              gatgGTATTTCCGGTTCGATAATGCTTGTACCAACAGGGAGGGAATACGATGGCATTAACAGATATCAAAGTCAGAGCAGCCAAGCCAACGGATAAGCAATATTAGTTGACTGATGGTGGCGGTATGCATCTGCTTGtcc                                                                                                         >  1:737266/1‑140 (MQ=255)                                tgGTATTTCCGGTTCGATAATGCTTGTACCAACAGGGAGGGAATACGATGGCATTAACAGATATCAAAGTCAGAGCAGCCAAGCCAACGGATAAGCAATATTAGTTGACTGATGGTGGCGGTATGCATCTGCTTGtccat                                                                                                       >  1:132489/1‑140 (MQ=255)                                 ggTATTTCCGGTTCGATAATGCTTGTACCAACAGGGAGGGAATGCGATGGCATTAACAGATATCAAAGTCAGAGCAGCCAAGCCAACGGATAAGCAATATTAGTTGACTGATGGTGGCGGTATGc                                                                                                                     <  1:339428/125‑1 (MQ=255)                                 ggTATTTCCGGTTCGATAATGCTTGTACCAACAGGGAGGGAATACGATGGCATTAACAGATATCAAAGTCAGAGCAGCCAAGCCAACGGATAAGCAATATTAGTTGACTGATGGTGGCGGTATGCATCTGCTTGtccatc                                                                                                      >  1:263416/1‑140 (MQ=255)                                 ggTATTTCCGGTTCGATAATGCTTGTACCAACAGGGAGGGAATACGATGGCATTAACAGATATCAAAGTCAGAGCAGCCAAGCCAACGGATAAGCAATATTAGTTGACTGATGGTGGCGGTATGCATCTGCTTGtccatc                                                                                                      >  2:801774/1‑140 (MQ=255)                                 ggTATTTCCGGTTCGATAATGCTTGTACCAACAGGAAGGGAATACGATGGCATTAACAGATATCAAAGTCAGAGCAGCCAAGCCAACGGATAAGCAATATTAGTTGACTGATGGTGGCGGTATGc                                                                                                                     >  2:339428/1‑125 (MQ=255)                                     tttCTGGTTCGATAATGCTTGTACCAACAGGGAGGGAATACGATGGCATTAACAGATATCAAAGTCAGAGCAGCCAAGCCAACGGATAAGCAATATTAGTTGACTGATGGTGGCGGTATGCATCTGCTTGTCCATCCaaa                                                                                                  <  1:265259/140‑1 (MQ=255)                                     tttCCGGTTCGATAATGCTTGTACCAACAGGGAGGGAATACGATGGCATTAACAGATATCAAAGTCAGAGCAGCCAAGCCAACGGATAAGCAATATTAGTTGACTGATGGTGGCGGTATGCATCTGCTTGTCCATCCaaa                                                                                                  <  1:801774/140‑1 (MQ=255)                                     tttCCGGTTCGATAATGCTTGTACCAACAGGGAGGGAATACGATGGCATTAACAGATATCAAAGTCAGAGCAGCCAAGCCAACGGATAAGCAATATTAGTTGACTGATGGTGGCGGTATGCATCTGCTTGTCCATCCaaa                                                                                                  >  1:7077/1‑140 (MQ=255)                                     tttCCGGTTCGATAATGCTTGTACCAACAGGGAGGGAATACGATGGCATTAACAGATATCAAAGTCAGAGCAGCCAAGCCAACGGATAAGCAATATTAGTTGACTGATGGTGGCGGTATGCATCTGCTTGTCCATCCaaa                                                                                                  <  2:132489/140‑1 (MQ=255)                                      ttCCGGTTCGATAATGCTTGTACCAACAGGGAGGGAATACGATGGCATTAACAGATATCAAAGTCAGAGCAGCCAAGCCAACGGATAAGCAATATTAGTTGACTGATGGTGGCGGTATGCATCTGCTTGTCCATCCaaa                                                                                                  <  2:375664/139‑1 (MQ=255)                                      ttCCGGTTCGATAATGCTTGTACCAACAGGGAGGGAATACGATGGCATTAACAGATATCAAAGTCAGAGCAGCCAAGCCAACGGATAAGCAATATTAGTTGACTGATGGTGGCGGTATGCATCTGCTTGTCCATCCaaa                                                                                                  >  1:375664/1‑139 (MQ=255)                                      ttCCGGTTCGATAATGCTTGTACCAACAGGGAGGGAATACGATGGCATTAACAGATATCAAAGTCAGAGCAGCCAAGCCAACGGATAAGCAATATTAGTTGACTGATGGTGGCGGTATGCATCTGCTTGTCCATCCAAAt                                                                                                 >  2:22894/1‑140 (MQ=255)                                       tCCGGTTCGATAATGCTTGTACCAACAGGGAGGGAATACGATGGCATTAACAGATATCAAAGTCAGAGCAGCCAAGCCAACGGATAAGCAATATTAGTTGACTGATGGTGGCGGTATGCATCTGCTTGTCCATCCAAATg                                                                                                <  1:22894/140‑1 (MQ=255)                                       tCCGGTTCGATAATGCTTGTACCAACAGGGAGGGAATACGATGGCATTAACAGATATCAAAGTCAGAGCAGCCAAGCCAACGGATAAGCAATATTAGTTGACTGATGGTGGCGGTATGCATCTGCTTGTCCATCCAAATg                                                                                                <  1:713964/140‑1 (MQ=255)                                            ttCGATAATGCTTGTACCAACAGGGAGGGAATACGATGGCATTAACAGATATCAAAGTCAGAGCAGCCAAGCCAACGGATAAGCAATATTAGTTGACTGATGGTGGCGGTATGCATCTGCTTGTCCATCCAAATGGTTCt                                                                                           <  2:576493/140‑1 (MQ=255)                                            ttCGATAATGCTTGTACCAACAGGGAGGGAATACGATGGCATTAACAGATATCAAAGTCAGAGCAGCCAAGCCAACGGATAAGCAATATTAGTTGACTGATGGTGGCGGTATGCATCTGCTTGTCCATCCAAATGGTTCt                                                                                           <  2:588037/140‑1 (MQ=255)                                                   aTGCTTGTACCAACAGGGAGGGAATACGATGGCATTAACAGATATCAAAGTCAGAGCAGCCAAGCCAACGGATAAGCAATATTAGTTGACTGATGGTGGCGGTATGCATCTGCTTGTCCATCCAAATGGTTCTAAGTACt                                                                                    <  1:36133/140‑1 (MQ=255)                                                      cTTGTACCAACAGGGAGGGAATACGATGGCATTAACAGATATCAAAGTCAGAGCAGCCAAGCCAACGGATAAGCAATATTAGTTGACTGATGGTGGCGGTATGCATCTGCTTGTCCATCCAAATGGTTCTAAGTACTGGc                                                                                 <  2:892893/140‑1 (MQ=255)                                                      cTTGTACCAACAGGGAGGGAATACGATGGCATTAACAGATATCAAAGTCAGAGCAGCCAAGCCAACGGATAAGCAATATTAGTTGACTGATGGTGGCGGTATGCATCTGCTTGTCCATCCAAATGGTTCTAAGTACTGGc                                                                                 >  2:896386/1‑140 (MQ=255)                                                      cTTGTACCAACAGGGAGGGAATACGATGGCATTAACAGATATCAAAGTCAGAGCAGCCAAGCCAACGGATAAGCAATATTAGTTGACTGATGGTGGCGGTATGCATCTGCTTGTCCATCCAAATGGTTCTAAGTACTGGc                                                                                 <  2:142691/140‑1 (MQ=255)                                                      cTTGTACCAACAGGGAGGGAATACGATGGCATTAACAGATATCAAAGTCAGAGCAGCCAAGCCAACGGATAAGCAATATTAGTTGACTGATGGTGGCGGTATGCATCTGCTTGTCCATCCAAATGGTTCTAAGTACTGGc                                                                                 <  2:263416/140‑1 (MQ=255)                                                        tGTACCAACAGGGAGGGAATACGATGGCATTAACAGATATCAAAGTCAGAGCAGCCAAGCCAACGGATAAGCAATATTAGTTGACTGATGGTGGCGGTATGCATCTGCTTGTCCATCCAAATGGTTCTAAGTACTGGCGt                                                                               <  2:619465/140‑1 (MQ=255)                                                         gTACCAACAGGGAGGGAATACGATGGCATTAACAGATATCAAAGTCAGAGCAGCCAAGCCAACGGATAAGCAATATTAGTTGACTGATGGTGGCGGTATGCATCTGCTTGTCCATCCAAATGGTTCTAAGTACTGGc                                                                                 <  2:748450/137‑1 (MQ=255)                                                         gTACCAACAGGGAGGGAATACGATGGCATTAACAGATATCAAAGTCAGAGCAGCCAAGCCAACGGATAAGCAATATTAGTTGACTGATGGTGGCGGTATGCATCTGCTTGTCCATCCAAATGGTTCTAAGTACTGGc                                                                                 >  1:748450/1‑137 (MQ=255)                                                             cAACAGGGAGGGAATACGATGGCATTAACAGATATCAAAGTCAGAGCAGCCAAGCCAACGGATAAGCAATATTAGTTGACTGATGGTGGCGGTATGCATCTGCTTGTCCATCCAAATGGTTCTAAGTACTGGCGTTTGCa                                                                          <  2:797765/140‑1 (MQ=255)                                                              aaCAGGGAGGGAATACGATGGCATTAACAGATATCAAAGTCAGAGCAGCCAAGCCAACGGATAAGCAATATTAGTTGACTGATGGTGGCGGTATGCATCTGCTTGTCCATCCAAATGGTTCTAAGTACTGGCGTTTGCA‑g                                                                        >  1:562668/1‑140 (MQ=255)                                                               aCAGGGAGGGAATACGATGGCATTAACAGATATCAAAGTCAGAGCAGCCAAGCCAACGGATAAGCAATATTAGTTGACTGATGGTGGCGGTATGCATCTGCTTGTCCATCCAAATGGTTCTAAGTACTGGCGTTTGCa                                                                          <  1:121971/138‑1 (MQ=255)                                                               aCAGGGAGGGAATACGATGGCATTAACAGATATCAAAGTCAGAGCAGCCAAGCCAACGGATAAGCAATATTAGTTGACTGATGGTGGCGGTATGCATCTGCTTGTCCATCCAAATGGTTCTAAGTACTGGCGTTTGCa                                                                          >  2:121971/1‑138 (MQ=255)                                                                cAGGGAGGGAATACGATGGCATTAACAGATATCAAAGTCAGAGCAGCCAAGCCAACGGATAAGCAATATTAGTTGACTGATGGTGGCGGTATGCATCTGCTTGTCCATCCAAATGGTTCTAAGTACTGGCGTTTGCA‑GTa                                                                      <  2:97981/140‑1 (MQ=255)                                                                 agggagggAATACGATGGCATTAACAGATATCAAAGTCAGAGCAGCCAAGCCAACGGATAAGCAATATTAGTTGACTGATGGTGGCGGTATGCATCTGCTTGTCCATCCAAATGGTTCTAAGTACTGGCGTTTGCA‑GTAc                                                                     <  1:409705/140‑1 (MQ=255)                                                                 agggagggAATACGATGGCATTAACAGATATCAAAGTCAGAGCAGCCAAGCCAACGGATAAGCAATATTAGTTGACTGATGGTGGCGGTATGCATCTGCTTGTCCATCCAAATGGTTCTAAGTACTGGCGTTTGCA‑GTAc                                                                     <  1:896386/140‑1 (MQ=255)                                                                   ggagggaATACGATGGCATTAACAGATATCAAAGTCAGAGCAGCCAAGCCAACGGATAAGCAATATTAGTTGACTGATGGTGGCGGTATGCATCTGCTTGTCCATCCAAATGGTTCTAAGTACTGGCGTTTGCA‑GTACCg                                                                   >  2:889043/1‑140 (MQ=255)                                                                   ggagggaATACGATGGCATTAACAGATATCAAAGTCAGAGCAGCCAAGCCAACGGATAAGCAATATTAGTTGACTGATGGTGGCGGTATGCATCTGCTTGTCCATCCAAATGGTTCTAAGTACTGGCGTTTGCA‑GTACCg                                                                   <  1:875804/140‑1 (MQ=255)                                                                                   cATTAACAGATATCAAAGTCAGAGCAGCCAAGCCAACGGATAAGCAATATTAGTTGACTGATGGTGGCGGTATGCATCTGCTTGTCCATCCAAATGGTTCTAAGTACTGGCGTTTGCA‑GTACCGTTATGAGGGAAAGCaa                                                   >  1:857146/1‑140 (MQ=255)                                                                                   cATTAACAGATATCAAAGTCAGAGCAGCCAAGCCAACGGATAAGCAATATTAGTTGACTGATGGTGGCGGTATGCATCTGCTTGTCCATCCAAATGGTTCTAAGTACTGGCGTTTGCA‑GTACCGTTATGAGGGAAAGCaa                                                   >  1:232342/1‑140 (MQ=255)                                                                                   cATTAACAGATATCAAAGTCAGAGCAGCCAAGCCAACGGATAAGCAATATTAGTTGACTGATGGTGGCGGTATGCATCTGCTTGTCCATCCAAATGGTTCT‑AGTACTGGGGGTTGCAGGAACGGTTTTGGGGGAAGGCaa                                                   >  1:804847/1‑140 (MQ=255)                                                                                     ttAACAGATATCAAAGTCAGAGCAGCCAAGCCAACGGATAAGCAATATTAGTTGACTGATGGTGGCGGTATGCATCTGCTTGTCCATCCAAATGGTTCTAAGTACTGGCGTTTGCA‑GTACCGTTATGAGGGAAAGCaaaa                                                 >  1:352053/1‑140 (MQ=255)                                                                                       aaCAGATATCAAAGTCAGAGCAGCCAAGCCAACGGATAAGCAATATTAGTTGACTGATGGTGGCGGTATGCATCTGCTTGTCCATCCAAATGGTTCTAAGTACTGGCGTTTGCA‑GTACCGTTATGAGGGAAAGCaaaaaa                                               >  2:690376/1‑140 (MQ=255)                                                                                         cAGATATCAAAGTCAGAGCAGCCAAGCCAACGGATAAGCAATATTAGTTGACTGATGGTGGCGGTATGCATCTGCTTGTCCATCCAAATGGTTCTAAGTACTGGCGTTTGCA‑GTACCGTTATGAGGGAAAGCAAAAAATg                                             <  1:70520/140‑1 (MQ=255)                                                                                           gATATCAAAGTCAGAGCAGCCAAGCCAACGGATAAGCAATATTAGTTGACTGATGGTGGCGGTATGCATCTGCTTGTCCATCCAAATGGTTCTAAGTACTGGCGTTTGCA‑GTACCGTTATGAGGGAAAGCAAAAAATGCt                                           <  1:874140/140‑1 (MQ=255)                                                                                            atatCAAAGTCAGAGCAGCCAAGCCAACGGATAAGCAATATTAGTTGACTGATGGTGGCGGTATGCATCTGCTTGTCCATCCAAATGGTTCTAAGTACTGGCGTTTGCA‑GTACCGTTATGAGGGAAAGCAAAAAATGCTg                                          >  1:205056/1‑140 (MQ=255)                                                                                                   aGTCAGAGCAGCCAAGCCAACGGATAAGCAATATTAGTTCACTGATGGTGGCGGTATGCATCTGCTTGTCCATCCAAATGGTTCTAAGTACTGGCGTTTGCA‑GTACCGTTATGAGGGAAAGCAAAAAATGCTGGCACTTg                                   >  1:39759/1‑140 (MQ=255)                                                                                                        gagCAGCCAAGCCAACGGATAAGCAATATTAGTTGACTGATGGTGGCGGTATGCATCTGCTTGTCCATCCAAATGGTTCTAAGTACTGGCGTTTGCA‑GTACCGTTATGAGGGAAAGCAAAAAATGCTGGCACTTGGGGtt                              >  2:474467/1‑140 (MQ=255)                                                                                                        gagCAGCCAAGCCAACGGATAAGCAATATTAGTTGACTGATGGTGGCGGTATGCATCTGCTTGTCCATCCAAATGGTTCTAAGTACTGGCGTTTGCA‑GTACCGTTATGAGGGAAAGCAAAAAATGCTGGCACTTGGGGtt                              >  2:84175/1‑140 (MQ=255)                                                                                                         agcagcCAAGCCAACGGATAAGCAATATTAGTTGACTGATGGTGGCGGTATGCATCTGCTTGTCCATCCAAATGGTTCTAAGTACTGGCGTTTGCA‑GTACCGTTATGAGGGAAAGCAAAAAATGCTGGCACTTGGGGttt                             >  2:637427/1‑140 (MQ=255)                                                                                                             gccaagccaaCGGATAAGCAATATTAGTTGACTGATGGTGGCGGTATGCATCTGCTTGTCCATCCAAATGGTTCTAAGTACTGGCGTTTGCA‑GTACCGTTATGAGGGAAAGCAAAAAATGCTGGCACTTGGGGTTTATcc                         <  2:737266/140‑1 (MQ=255)                                                                                                               caagccaaCGGATAAGCAATATTAGTTGACTGATGGTGGCGGTATGCATCTGCTTGTCCATCCAAATGGTTCTAAGTACTGGCGTTTGCA‑GTACCGTTATGAGGGAAAGCAAAAAATGCTGGCACTTGGGGTTTATCCTg                       >  1:796005/1‑140 (MQ=255)                                                                                                                 agccaaCGGATAAGCAATATTAGTTGACTGATGGTGGCGGTATGCATCTGCTTGTCCATCCAAATGGTTCTAAGTACTGGCGTTTGCA‑GTACCGTTATGAGGGAAAGCAAAAAATGCTGGCACTTGGGGTTTATCCTGaa                     >  1:946140/1‑140 (MQ=255)                                                                                                                   ccaaCGGATAAGCAATATTAGTTGACTGATGGTGGCGGTATGCATCTGCTTGTCCATCCAAATGGTTCTAAGTACTGGCGTTTGCA‑GTACCGTTATGAGGGAAAGCAAAAAATGCTGGCACTTGGGGTTTATCCTGAAAt                   <  2:562668/140‑1 (MQ=255)                                                                                                                      aCGGATAAGCAATATTAGTTGACTGATGGTGGCGGTATGCATCTGCTTGTCCATCCAAATGGTTCTAAGTACTGGCGTTTGCA‑GTACCGTTATGAGGGAAAGCAAAAAATGCTGGCACTTGGGGTTTATCCTGAAATcac                >  1:191157/1‑140 (MQ=255)                                                                                                                        ggATAAGCAATATTAGTTGACTGATGGTGGCGGTATGCATCTGCTTGTCCATCCAAATGGTTCTAAGTACTGGCGTTTGCA‑GTACCGTTATGAGGGAAAGCAAAAAATGCTGGCACTTGGGGTTTATCCTGAAATCacac              <  2:796005/140‑1 (MQ=255)                                                                                                                        ggATAAGCAATATTAGTTGACTGATGGTGGCGGTATGCATCTGCTTGTCCATCCAAATGGTTCTAAGTACTGGCGTTTGCA‑GTACCGTTATGAGGGAAAGCAAAAAATGCTGGCACTTGGGGTTTATCCTGAAATCacac              <  2:649062/140‑1 (MQ=255)                                                                                                                            aaGCAATATTAGTTGACTGATGGTGGCGGTATGCATCTGCTTGTCCATCCAAATGGTTCTAAGTACTGGCGTTTGCA‑GTACCGTTATGAGGGAAAGCAAAAAATGCTGGCACTTGGGGTTTATCCTGAAATCACACTAGc          <  1:637427/140‑1 (MQ=255)                                                                                                                            aaGCAATATTAGTTCACTGATGGTGGCGGTATGCATCTGCTTGTCCATCCAAATGGTTCTAAGTACTGGCGTTTGCA‑GTACCGTTATGAGGGAAAGCAAAAAATGCTGGCACTTGGGGTTTATCCTGAAATCACACTAGc          <  2:39759/140‑1 (MQ=255)                                                                                                                               cAATATTAGTTGGCTGATGGTGGCGGTATGCATCTGCTTGTCCATCCAAATGTTTCTAAGTACTGGCGTTTGCA‑GTACCGTTATGAGGGAAAGCAAGAAATGCTGGCACTTGGGGTGTATCCTGAAATCACACTAGCGGa       <  2:7077/140‑1 (MQ=255)                                                                                                                               cAATATTAGTTGACTGATGGTGGCGGTATGCATCTGCTTGTCCATCCAAATGGTTCTAAGTACTGGCGTTTGCA‑GTACCGTTATGAGGGAAAGCAAAAAATGCTGGCACTTGGGGTTTATCCTGAAATCACACTAGCGGa       <  1:690376/140‑1 (MQ=255)                                                                                                                               cAATATTAGTTGACTGATGGTGGCGGTATGCATCTGCTTGTCCATCCAAATGGTTCTAAGTACTGGCGTTTGCA‑GTACCGTTATGAGGGAAAGCAAAAAATGCTGGCACTTGGGGTTTATCCTGAAATCACACTAGCGGa       <  2:946140/140‑1 (MQ=255)                                                                                                                                    ttAGTTGACTGATGGTGGCGGTATGCATCTGCTTGTCCATCCAAATGGTTCTAAGTACTGGCGTTTGCA‑GTACCGTTATGAGGGAAAGCAAAAAATGCTGGCACTTGGGGTTTATCCTGAAATCACACTAGCGGATGCCa  <  2:352053/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                         TACATCTACCAACATATGTTGGTACAGATGATGGTATTTCCGGTTCGATAATGCTTGTACCAACAGGGAGGGAATACGATGGCATTAACAGATATCAAAGTCAGAGCAGCCAAGCCAACGGATAAGCAATATTAGCTGACTGATGGTGGCGGTATGCATCTGCTTGTCCATCCAAATGGTTCTAAGTACTGGCGTTTGCA‑GTACCGTTATGAGGGAAAGCAAAAAATGCTGGCACTTGGGGTTTATCCTGAAATCACACTAGCGGATGCCA  >  NC_000913/4496597‑4496867

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑