Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,404,2150TG30.2% 70.4 / 16.9 43G244G (GGT→GGG) yhdHacrylyl‑CoA reductase
Reads supporting (aligned to +/- strand):  ref base T (5/25);  new base G (13/0);  total (18/25)
Fisher's exact test for biased strand distribution p-value = 2.34e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

GATGAGTTTGCCGAATCCCGTCCTCTGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATGTCCGTTTGCAAGGGGTGGATTCAGTAATGACGCCACCAGAACGCCGCGCACAAGCCTGGCAGCGACTGGTCGCCGATTTACCGGAATCATT  >  NC_000913/3404078‑3404352
                                                                                                                                         |                                                                                                                                         
gatgaGTTTGCCGAATCCCGTCCTCTGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGggtgg                                                                                                                                         >  1:377105/1‑140 (MQ=255)
     gTTTGCCGAATCCCGTCCTCTGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTa                                                                                                                                    <  2:521129/140‑1 (MQ=255)
        tGCCGAATCCCGTCCTCTGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGGGGGTTTtctc                                                                                                                                 >  2:621324/1‑140 (MQ=255)
          ccGAATCCCGTCCTCTGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTg                                                                                                                               <  1:821163/140‑1 (MQ=255)
            gAATCCCGTCCTCTGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGcc                                                                                                                             <  2:144437/140‑1 (MQ=255)
               tCCCGTCCTCTGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGGCTGGCGGGGGGGTTTTTTCTGCCAAc                                                                                                                          >  2:31524/1‑140 (MQ=255)
                    tCCTCTGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGGGGGTTTACTCTGCCAACCACgg                                                                                                                     >  1:775105/1‑140 (MQ=255)
                      ctctGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTc                                                                                                                   <  1:666850/140‑1 (MQ=255)
                        ctGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCAt                                                                                                                 <  2:546918/140‑1 (MQ=255)
                        ctGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCAt                                                                                                                 <  1:31524/140‑1 (MQ=255)
                            aaaaaCAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCATGCCa                                                                                                             <  2:377105/140‑1 (MQ=255)
                                 cAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTAt                                                                                                        >  1:826310/1‑140 (MQ=255)
                                 cAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGGGGGTTTACTCTGCCAACCACGGTCATGCCAttttt                                                                                                        >  1:675890/1‑138 (MQ=255)
                                     tCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTg                                                                                                    <  1:621324/140‑1 (MQ=255)
                                     tCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTg                                                                                                    <  1:197039/140‑1 (MQ=255)
                                             ggggCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATGt                                                                                            >  1:308412/1‑140 (MQ=255)
                                               ggCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATGTcc                                                                                          <  2:357025/140‑1 (MQ=255)
                                                     tGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATGTCCGTTTGc                                                                                    <  1:886074/140‑1 (MQ=255)
                                                     tGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATGTCCGTTTGc                                                                                    <  1:730276/140‑1 (MQ=255)
                                                        cacCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGGGGGTTTTCTCTGCCAACCACGGGCATGCCATTTTTTCTGCGGTATGTCCGTTTGGAAg                                                                                 >  2:568135/1‑140 (MQ=255)
                                                            gTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATGTCCGTTTGCAAGGGGt                                                                             <  1:568135/140‑1 (MQ=255)
                                                            gTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATGTCCGTTTGCAAGGGGt                                                                             <  1:642996/140‑1 (MQ=255)
                                                              tGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGGTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATGTCCGTTTGCAAGGGGTgg                                                                           >  1:729631/1‑140 (MQ=255)
                                                              tGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGGGGGTTTTCTCTGCCCACCACGGGCATGCCATTTATTTTGCGTAATGTCCGTTTTCAAGgggggg                                                                           >  2:174262/1‑140 (MQ=255)
                                                              tGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGGGGGTTTTCTCTGCCAACCCCGGGCATGCCCTTTTTTTTGCGTAATGTCCGTTTGCAAGgggggg                                                                           >  2:730276/1‑140 (MQ=255)
                                                              tGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGGGGGTTTTCTCTGCCAACCCCGGGCATGCCCTTTTTTCTGCGGAATGTCCGTTTTCAAGgggggg                                                                           >  1:935165/1‑140 (MQ=255)
                                                              tGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGGGGGTTTACTCTTCCAACCACGGTCATGCCATTTATTCTGCGTAATGGCCGTTTGCAAGgggggg                                                                           >  1:721155/1‑140 (MQ=255)
                                                               ggCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATGTCCGTTTGCAAGGGGTGGa                                                                          <  1:797374/140‑1 (MQ=255)
                                                               ggCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATGTCCGTTTGCAAGGGGTGGa                                                                          <  1:756524/140‑1 (MQ=255)
                                                               ggCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATGTCCGTTTGCAAGGGGTGGa                                                                          <  1:105144/140‑1 (MQ=255)
                                                               ggCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATGTCCGTTTGCAAGGGGTGGa                                                                          <  1:174262/140‑1 (MQ=255)
                                                               ggCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGGGGGTTTTCTCTGCCAACCACGGGCATGCCATTTATTCTGCGTAATGTCCGGTTGCAAGggggggg                                                                          >  2:850340/1‑139 (MQ=255)
                                                                gCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGGGGGTTTTCTCTGCCAACCACGGGCATGCCATTTATTCTGCGGAATGTCCGTTTTGAAGgggggggt                                                                         >  2:756524/1‑138 (MQ=255)
                                                                gCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGGGGGTTTTATCTGCCCACCCCGGGCCTGCCATTTTTTTTGCGTAATGTCCGTTTGGCAGgggggggt                                                                         >  2:48143/1‑138 (MQ=255)
                                                                 cGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGGGGGTTTTCTCTACCAACCACGGGCATGCCCTTTTTTTTGCGTAATGTCCGTTTTCAAGGGGGGGAtt                                                                        >  2:732739/1‑140 (MQ=255)
                                                                                   aGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGGGGGTTTTCTCTGCCCACCCCGGGCCTGCCCATTTTTCTGCGTAATGTCCGGTTTCAAGGGGGGGGTTCAGTAAAGACGCCACCAg                                                      >  2:786130/1‑140 (MQ=255)
                                                                                        tGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCCACCACGGGCATGCCATTTATTCTGCGTAATGTCCGTTTGCAAGGGGTGGATTTAGTAATGACGCCACCAGAAcgc                                                 >  1:721408/1‑140 (MQ=255)
                                                                                         ggCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATGTCCGTTTGCAAGGGGTGGATTCAGTAATGACGCCACCAGAAcgcc                                                <  2:826310/140‑1 (MQ=255)
                                                                                             cAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATGTCCGTTTGCAAGGGGTGGATTCAGTAATGACGCCACCAGAACGCCgcgc                                            <  1:550468/140‑1 (MQ=255)
                                                                                               aaTGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATGTCCGTTTGCAAGGGGTGGATTCAGTAATGACGCCACCAGAACGCCGCGcac                                          <  1:850340/140‑1 (MQ=255)
                                                                                                        cggcggCTGCGTGGCGGCCTGTGGTCTGGCGGGGGGGTTTTCTCTGCCAACCACGGGCATGCCATTTTTTTTGCGTAATGTCCGTTTTCAAGGGGGGGGTTTAGTAATGACGCCACCAGAACGCCGCGCCCAAACCCGGc                                 >  2:550468/1‑140 (MQ=255)
                                                                                                          gcggcTGCGTGGCGGCCTGTGGTCTGGCGGGGGGGTTTTCTCTTCCCACCACCGGCATTCCCTTTATTCTGCGGAATGTCCGTTTTCAAGGGGGGGATTTAGTAATGACGCCCCCCGAACGCCCCGCCCAAACCTTGccg                               >  2:686291/1‑138 (MQ=255)
                                                                                                             gcTGCGTGGCGGCCTGTGGTCTGGCGGGGGGTTTTTCTCTGCCAACCACGGGCATGCCCTTTTTTTTGCGTAATGTCCGTTTGGAAGGGGGGGGTTTAGTAATGACGCCACCAGAACGCCGCGCACAAGCCTGGCAGCGa                            >  2:174643/1‑140 (MQ=255)
                                                                                                             gcTGCGTGGCGGCCTGTGGTCTGGCGGGGGGTTTTTATCTGCCAAACCCGGGCATGCCCTTTATTCTGGGGAAAGTCCGTTTGGAAGGGGGGGATTTAGTAATGACGCCCCCAGAAAGCCGCGCACAAGCCTGGCAGCgg                            >  2:835407/1‑139 (MQ=255)
                                                                                                                         ccTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATGTCCGTTTGCAAGGGGTGGATTCAGTAATGACGCCACCAGAACGCCGCGCACAAGCCTGGCAGCGACTGGTCGCCGAt                <  1:441788/140‑1 (MQ=255)
                                                                                                                          cTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATGTCCGTTTGCAAGGGGTGGATTCAGTAATGACGCCACCAGAACGCCGCGCACAAGCCTGGCAGCGACTGGTCGCCGAtt               <  1:933502/140‑1 (MQ=255)
                                                                                                                                   ggCGGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATGTCCGTTTGCAAGGGGTGGATTCAGTAATGACGCCACCAGAACGCCGCGCACAAGCCTGGCAGCGACTGGTCGCCGATTTACCGGAAt      >  2:44708/1‑140 (MQ=255)
                                                                                                                                   ggCGGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATGTCCGTTTGCAAGGGGTGGATTCAGTAATGACGCCACCAGAACGCCGCGCACAAGCCTGGCAGCGACTGGTCGCCGATTTACCGGAAt      <  2:675890/140‑1 (MQ=255)
                                                                                                                                   ggCGGGTGGTTTTACTCTACCAACCACGGTCATGCCATTTATTCTGCGTAATGTCCGTTTGCAAGGGGTGGATTCAGTAATGACGCCACCAGAACGCCGCGCACAAGCCTGGCAGCGACTGGTCGCCGATTTACCGGAAt      <  1:732739/140‑1 (MQ=255)
                                                                                                                                       ggtggtTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATGTCCGTTTGCAAGGGGTGGATTCAGTAATGACGCCACCAGAACGCCGCGCACAAGCCTGGCAGCGACTGGTCGCCGATTTACCGGAATCAtt  <  1:44708/140‑1 (MQ=255)
                                                                                                                                       ggtggtTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATGTCCGTTTGCAAGGGGTGGATTCAGTAATGACGCCACCAGAACGCCGCGCACAAGCCTGGCAGCGACTGGTCGCCGATTTACCGGAATCAtt  <  1:140355/140‑1 (MQ=255)
                                                                                                                                       ggtggtTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATGTCCGTTTGCAAGGGGTGGATTCAGTAATGACGCCACCAGAACGCCGCGCACAAGCCTGGCAGCGACTGGTCGCCGATTTACCGGAATCAtt  >  1:122617/1‑140 (MQ=255)
                                                                                                                                         |                                                                                                                                         
GATGAGTTTGCCGAATCCCGTCCTCTGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATGTCCGTTTGCAAGGGGTGGATTCAGTAATGACGCCACCAGAACGCCGCGCACAAGCCTGGCAGCGACTGGTCGCCGATTTACCGGAATCATT  >  NC_000913/3404078‑3404352

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑