Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,329,057	C→T	V247V (GTG→GTA)	yfaQ ←	tandem DUF2300 domain‑containing protein YfaQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,329,057	0	C	T	97.8%	153.6 / ‑3.7	46	V247V (GTG→GTA)	yfaQ	tandem DUF2300 domain‑containing protein YfaQ
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base T (26/19);  total (26/20)
Fisher's exact test for biased strand distribution p-value = 4.35e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.90e-01

CGTCACACGATAGCGCCCATTAAGTACGCCGGGATTTACTGCCGTCGTACTCTTTTCCGCTGTGATTTTCTTCAGCGGATAGCGGGCAAACAGCTCCACCTCTACACATTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCCACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCAT  >  NC_000913/2328925‑2329193                                                                                                                                     |                                                                                                                                         cGTCACACGATAGCGCCCATTAAGTACGCCGGGATTTACTGCCGTCGTACTCTTTTCCGCTGTGATTTTCTTCAGCGGATAGCGGGCAAACAGCTCCACCTCTACACATTGCCCACTGGCAACCTGCGTCGGTACTGGCa                                                                                                                                   >  1:185822/1‑140 (MQ=255)    cacaCGATAGCGCCCATTAAGTACGCCGGGATTTACTGCCGTCGTACTCTTTTCCGCTGTGATTTTCTTCAGCGGATAGCGGGCAAACAGCTCCACCTCTACACATTGCCCACTGGCAACCTGCGTCGGTACTGGCaaaa                                                                                                                                >  1:314882/1‑140 (MQ=255)       acGATAGCGCCCATTAAGTACGCCGGGATTTACTGCCGTCGTACTCTTTTCCGCTGTGATTTTCTTCAGCGGATAGCGGGCAAACAGCTCCACCTCTACACATTGCCCACTGGCAACCTGCGTCGGTACTGGCAAAAccc                                                                                                                             >  2:627306/1‑140 (MQ=255)       acGATAGCGCCCATTAAGTACGCCGGGATTTACTGCCGTCGTACTCTTTTCCGCTGTGATTTTCTTCAGCGGATAGCGGGCAAACAGCTCCACCTCTACACATTGCCCACTGGCAACCTGCGTCGGTACTGGCAAAAccc                                                                                                                             >  1:299383/1‑140 (MQ=255)                 ccATTAAGTACGCCGGGATTTACTGCCGTCGTACTCTTTTCCGCTGTGATTTTCTTCAGCGGATAGCGGGCAAACAGCTCCACCTCTACACATTGCCCACTGGCAACCTGCGTCGGTACTGGCAAAACCCGATTAAGCAc                                                                                                                   <  1:627306/140‑1 (MQ=255)                     tAAGTACGCCGGGATTTACTGCCGTCGTACTCTTTTCCGCTGTGATTTTCTTCAGCGGATAGCGGGCAAACAGCTCCACCTCTACACATTGCCCACTGGCAACCTGCGTCGGTACTGGCAAAACCCGATTAAGCACCGTc                                                                                                               >  2:719800/1‑140 (MQ=255)                           cGCCGGGATTTACTGCCGTCGTACTCTTTTCCGCTGTGATTTTCTTCAGCGGATAGCGGGCAAACAGCTCCACCTCTACACATTGCCCACTGGCAACCTGCGTCGGTACTGGCAAAACCCGATTAAGCACCGTCGCATaa                                                                                                         <  1:936231/140‑1 (MQ=255)                             ccGGGATTTACTGCCGTCGTACTCTTTTCCGCTGTGATTTTCTTCAGCGGATAGCGGGCAAACAGCTCCACCTCTACACATTGCCCACTGGCAACCTGCGTCGGTACTGGCAAAACCCGATTAAGCACCGTCGCATAACg                                                                                                       >  1:228430/1‑140 (MQ=255)                              cGGGATTTACTGCCGTCGTACTCTTTTCCGCTGTGATTTTCTTCAGCGGATAGCGGGCAAACAGCTCCACCTCTACACATTGCCCACTGGCAACCTGCGTCGGTACTGGCAAAACCCGATTAAGCACCGTCGCATAACgg                                                                                                      >  2:391704/1‑140 (MQ=255)                               gggATTTACTGCCGTCGTACTCTTTTCCGCTGTGATTTTCTTCAGCGGATAGCGGGCAAACAGCTCCACCTCTACACATTGCCCACTGGCAACCTGCGTCGGTACTGGCAAAACCCGATTAAGCACCGTCGCATAACggg                                                                                                     >  1:76316/1‑140 (MQ=255)                                 gATTTACTGCCGTCGTACTCTTTTCCGCTGTGATTTTCTTCAGCGGATAGCGGGCAAACAGCTCCACCTCTACACATTGCCCACTGGCAACCTGCGTCGGTACTGGCAAAACCCGATTAAGCACCGTCGCATaa                                                                                                         <  2:944133/134‑1 (MQ=255)                                 gATTTACTGCCGTCGTACTCTTTTCCGCTGTGATTTTCTTCAGCGGATAGCGGGCAAACAGCTCCACCTCTACACATTGCCCACTGGCAACCTGCGTCGGTACTGGCAAAACCCGATTAAGCACCGTCGCATaa                                                                                                         >  1:944133/1‑134 (MQ=255)                                    ttACTGCCGTCGTACTCTTTTCCGCTGTGATTTTCTTCAGCGGATAGCGGGCAAACAGCTCCACCTCTACACATTGCCCACTGGCAACCTGCGTCGGTACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGt                                                                                                    <  2:508939/136‑1 (MQ=255)                                    ttACTGCCGTCGTACTCTTTTCCGCTGTGATTTTCTTCAGCGGATAGCGGGCAAACAGCTCCACCTCTACACATTGCCCACTGGCAACCTGCGTCGGTACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGt                                                                                                    >  1:508939/1‑136 (MQ=255)                                      aCTGCCGTCGTACTCTTTTCCGCTGTGATTTTCTTCAGCGGATAGCGGGCAAACAGCTCCACCTCTACACATTGCCCACTGGCAACCTGCGTCGGTACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTAc                                                                                              >  2:76242/1‑140 (MQ=255)                                          ccGTCGTACTCTTTTCCGCTGTGATTTTCTTCAGCGGATAGCGGGCAAACAGCTCCACCTCTACACATTGCCCACTGGCAACCTGCGTCGGTACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGtt                                                                                          >  2:308539/1‑140 (MQ=255)                                           cgtcgtACTCTTTTCCGCTGTGATTTTCTTCAGCGGATAGCGGGCAAACAGCTCCACCTCTACACATTGCCCACTGGCAACCTGCGTCGGTACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGttt                                                                                         >  1:489846/1‑140 (MQ=255)                                           cgtcgtACTCTTTTCCGCTGTGATTTTCTTCAGCGGATAGCGGGCAAACAGCTCCACCTCTACACATTGCCCACTGGCAACCTGCGTCGGTACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGttt                                                                                         >  1:926864/1‑140 (MQ=255)                                                             tgtgATTTTCTTCAGCGGATAGCGGGCAAACAGCTCCACCTCTACACATTGCCCACTGGCAACCTGCGTCGGTACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAg                                                                       <  2:314882/140‑1 (MQ=255)                                                               tgATTTTCTTCAGCGGATAGCGGGCAAACAGCTCCACCTCTACACATTGCCCACTGGCAACCTGCGTCGGTACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTa                                                                     <  1:431933/140‑1 (MQ=255)                                                                    ttcttcAGCGGATAGCGGGCAAACAGCTCCACCTCTACACATTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCca                                                                <  1:23817/140‑1 (MQ=255)                                                                       ttcAGCGGATAGCGGGCAAACAGCTCCACCTCTACACATTGCCCACTGGCAACCTGCGTCGGTACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCCACAg                                                             <  2:299383/140‑1 (MQ=255)                                                                       ttcAGCGGATAGCGGGCAAACAGCTCCACCTCTACACATTGCCCACTGGCAACCTGCGTCGGTACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCCACAg                                                             <  2:38791/140‑1 (MQ=255)                                                                                tAGCGGGCAAACAGCTCCACCTCTACACATTGCCCACTGGCAACCTGCGTCGGTACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCCACAGCGGTGTAcc                                                    <  1:719800/140‑1 (MQ=255)                                                                                tAGCGGGCAAACAGCTCCACCTCTACACATTGCCCACTGGCAACCTGCGTCGGTACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCCACAGCGGTGTAcc                                                    <  2:660051/140‑1 (MQ=255)                                                                                        aaaCAGCTCCACCTCTACACATTGCCCACTGGCAACCTGCGTCGGTACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCCACAGCGGTGTACCATCGGTTa                                            >  1:756300/1‑140 (MQ=255)                                                                                            aGCTCCACCTCTACACATTGCCCACTGGCAACCTGCGTCGGTACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCCACAGCGGTGTACCATCGGTTAAcca                                        <  1:308539/140‑1 (MQ=255)                                                                                                 cACCTCTACACATTGCCCACTGGCAACCTGCGTCGGTACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCCACAGCGGTGTACCATCGGTTAAccagccag                                   <  2:76316/140‑1 (MQ=255)                                                                                                      ctACACATTGCCCACTGGCAACCTGCGTCGGTACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCCACAGCGGTGTACCATCGGTTAACCAGCCAGCaaaa                              >  2:492904/1‑140 (MQ=255)                                                                                                       tACACATTGCCCACTGGCAACCTGCGTCGGTACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCCACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAac                             >  2:408337/1‑140 (MQ=255)                                                                                                           caTTGCCCACTGGCAACCTGCGTCGGTACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCCACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAaccacc                         >  1:495874/1‑140 (MQ=255)                                                                                                            aTTGCCCACTGGCAACCTGCGTCGGTACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCCACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCAccc                        <  2:284182/140‑1 (MQ=255)                                                                                                             ttGCCCACTGGCAACCTGCGTCGGTACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCCACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCt                       <  1:381772/140‑1 (MQ=255)                                                                                                             ttGCCCACTGGCAACCTGCGTCGGTACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCCACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCt                       <  2:489815/140‑1 (MQ=255)                                                                                                              tGCCCACTGGCAACCTGCGTCGGTACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCCACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAacca                           >  2:9986/1‑135 (MQ=255)                                                                                                              tGCCCACTGGCAACCTGCGTCGGTACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCCACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAacca                           <  1:9986/135‑1 (MQ=255)                                                                                                              tGCCCACTGGCAACCTGCGTCGGTACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCCACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTg                      >  2:158671/1‑140 (MQ=255)                                                                                                              tGCCCACTGGCAACCTGCGTCGGTACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCCACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTg                      >  2:720773/1‑140 (MQ=255)                                                                                                                cccACTGGCAACCTGCGTCGGTACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCCACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGAc                    >  2:137938/1‑140 (MQ=255)                                                                                                                cccACTGGCAACCTGCGTCGGTACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCCACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGAc                    >  2:131892/1‑140 (MQ=255)                                                                                                                    cTGGCAACCTGCGTCGGTACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCCACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGAt                <  1:408337/140‑1 (MQ=255)                                                                                                                     tGGCAACCTGCGTCGGTACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCCACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGAtt               >  2:620423/1‑140 (MQ=255)                                                                                                                               cGTCGGTACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCCACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTat     >  2:443395/1‑140 (MQ=255)                                                                                                                               cGTCGGTACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCCACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTat     >  2:358208/1‑140 (MQ=255)                                                                                                                                gTCGGTACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCCACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTatc    <  2:495874/140‑1 (MQ=255)                                                                                                                                  cGGTACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCCACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTatcat  <  2:185822/140‑1 (MQ=255)                                                                                                                                     |                                                                                                                                         CGTCACACGATAGCGCCCATTAAGTACGCCGGGATTTACTGCCGTCGTACTCTTTTCCGCTGTGATTTTCTTCAGCGGATAGCGGGCAAACAGCTCCACCTCTACACATTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCCACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCAT  >  NC_000913/2328925‑2329193

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑