| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,950,734 | A→G | R576R (CGT→CGC) | recD ← | exodeoxyribonuclease V subunit RecD |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,950,734 | 0 | A | G | 98.2% | 205.6 / ‑4.5 | 54 | R576R (CGT→CGC) | recD | exodeoxyribonuclease V subunit RecD |
| Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (28/25); total (29/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CGTAACACTCGTACGTCGCATCCGGCAATTACGTTTATTCCCGTGAACTAAACAACGCCGCCAGACCACTGCGCCGCTCAGTACGAGTGGCGATTGCCGCACTTAATATGCGCTCATCGGCATACAGCGACAGACGGCGACGCGCGCGGGTCACCGCGGTATAAACCAGCTCTCGCGTTACTACCGGCGTGCGTTGGCTCGGCAAAATCAACGCCGCATGGTCGAACTCCGATCCCTGCGATTTATGTACCGTCATCGCCCACGT > NC_000913/2950601‑2950865 | cGTAACACTCGTACGTCGCATCCGGCAATTACGTTTATTCCCGTGAACTAAACAACGCCGCCAGACCACTGCGCCGCTCAGTACGAGTGGCGATTGCCGCACTTAATATGCGCTCATCGGCATACAGCGACAGGCGGCGa > 2:431593/1‑140 (MQ=255) gTAACACTCGTACGTCGCATCCGGCAATTACGTTTATTCCCGTGAACTAAACAACGCCGCCAGACCACTGCGCCGCTCAGTACGAGTGGCGATTGCCGCACTTAATATGCGCTCATCGGCATACAGCGACAGGCGGCGAc < 1:67938/140‑1 (MQ=255) aaCACTCGTACGTCGCATCCGGCAATTACGTTTATTCCCGTGAACTAAACAACGCCGCCAGACCACTGCGCCGCTCAGTACGAGTGGCGATTGCCGCACTTAATATGCGCTCATCGGCATACAGCGACAGGCGGCGAcgc > 2:701693/1‑140 (MQ=255) acacTCGTACGTCGCATCCGGCAATTACGTTTATTCCCGTGAACTAAACAACGCCGCCAGACCACTGCGCCGCTCAGTACGAGTGGCGATTGCCGCACTTAATATGCGCTCATCGGCATACAGCGACAGGCGGCGAcgcg < 1:837089/140‑1 (MQ=255) acTCGTACGTCGCATCCGGCAATTACGTTTATTCCCGTGAACTAAACAACGCCGCCAGACCACTGCGCCGCTCAGTACGAGTGGCGATTGCCGCACTTAATATGCGCTCATCGGCATACAGCGACAGGCGGCGAcgcgcg < 2:42908/140‑1 (MQ=255) tCGTACGTCGCATCCGGCAATTACGTTTATTCCCGTGAACTAAACAACGCCGCCAGACCACTGCGCCGCTCAGTACGAGTGGCGATTGCCGCACTTAATATGCGCTCATCGGCATACAGCGACAGGCGGCGAcgcgcgcg > 2:197995/1‑140 (MQ=255) cGTACGTCGCATCCGGCAATTACGTTTATTCCCGTGAACTAAACAACGCCGCCAGACCACTGCGCCGCTCAGTACGAGTGGCGATTGCCGCACTTAATATGCGCTCATCGGCATACAGCGACAGGCgg < 2:473854/128‑1 (MQ=255) cGTACGTCGCATCCGGCAATTACGTTTATTCCCGTGAACTAAACAACGCCGCCAGACCACTGCGCCGCTCAGTACGAGTGGCGATTGCCGCACTTAATATGCGCTCATCGGCATACAGCGACAGGCgg > 1:473854/1‑128 (MQ=255) gTACGTCGCATCCGGCAATTACGTTTATTCCCGTGAACTAAACAACGCCGCCAGACCACTGCGCCGCTCAGTACGAGTGGCGATTGCCGCACTTAATATGCGCTCATCGGCATACAGCGACAGGCGGCGACGCGCGCggg > 1:279078/1‑140 (MQ=255) tCGCATCCGGCAATTACGTTTATTCCCGTGAACTAAACAACGCCGCCAGACCACTGCGCCGCTCAGTACGAGTGGCGATTGCCGCACTTAATATGCGCTCATCGGCATACAGCGACAGGCGGCGAcgc > 2:808482/1‑128 (MQ=255) tCGCATCCGGCAATTACGTTTATTCCCGTGAACTAAACAACGCCGCCAGACCACTGCGCCGCTCAGTACGAGTGGCGATTGCCGCACTTAATATGCGCTCATCGGCATACAGCGACAGGCGGCGAcgc < 1:808482/128‑1 (MQ=255) cGCATCCGGCAATTACGTTTATTCCCGTGAACTAAACAACGCCGCCAGACCACTGCGCCGCTCAGTACGAGTGGCGATTGCCGCACTTAATATGCGCTCATCGGCATACAGCGACAGGCGGCGACGCGCGCGGGTCACcg < 1:301219/140‑1 (MQ=255) cGCATCCGGCAATTACGTTTATTCCCGTGAACTAAACAACGCCGCCAGACCACTGCGCCGCTCAGTACGAGTGGCGATTGCCGCACTTAATATGCGCTCATCGGCATACAGCGACAGGCGGCGACGCGCGCGGGTCACcg < 2:459041/140‑1 (MQ=255) cGCATCCGGCAATTACGTTTATTCCCGTGAACTAAACAACGCCGCCAGACCACTGCGCCGCTCAGTACGAGTGGCGATTGCCGCACTTAATATGCGCTCATCGGCATACAGCGACAGGCGGCGACGCGCGCGGGTCACcg > 2:876593/1‑140 (MQ=255) cATCCGGCAATTACGTTTATTCCCGTGAACTAAACAACGCCGCCAGACCACTGCGCCGCTCAGTACGAGTGGCGATTGCCGCACTTAATATGCGCTCATCGGCATACAGCGACAGGCGGCGACGCGCGCGGGt > 2:147977/1‑133 (MQ=255) cATCCGGCAATTACGTTTATTCCCGTGAACTAAACAACGCCGCCAGACCACTGCGCCGCTCAGTACGAGTGGCGATTGCCGCACTTAATATGCGCTCATCGGCATACAGCGACAGGCGGCGACGCGCGCGGGt < 1:147977/133‑1 (MQ=255) cATCCGGCAATTACGTTTATTCCCGTGAACTAAACAACGCCGCCAGACCACTGCGCCGCTCAGTACGAGTGGCGATTGCCGCACTTAATATGCGCTCATCGGCATACAGCGACAGGCGGCGACGCGCGCGGGTCACcgcg > 1:331071/1‑140 (MQ=255) cATCCGGCAATTACGTTTATTCCCGTGAACTAAACAACGCCGCCAGACCACTGCGCCGCTCAGTACGAGTGGCGATTGCCGCACTTAATATGCGCTCATCGGCATACAGCGACAGGCGGCGACGCGCGCGGGTCACcgcg > 1:829129/1‑140 (MQ=255) tCCGGCAATTACGTTTATTCCCGTGAACTAAACAACGCCGCCAGACCACTGCGCCGCTCAGTACGAGTGGCGATTGCCGCACTTAATATGCGCTCATCGGCATACAGCGACAGGCGGCGACGCGCGCGGGTCACCGCGGt > 2:781929/1‑140 (MQ=255) ttACGTTTATTCCCGTGAACTAAACAACGCCGCCAGACCACTGCGCCGCTCAGTACGAGTGGCGATTGCCGCACTTAATATGCGCTCATCGGCATACAGCGACAGGCGGCGACGCGCGCGGGTCACCGCGGTATAAACCa > 2:331558/1‑140 (MQ=255) ttATTCCCGTGAACTAAACAACGCCGCCAGACCACTGCGCCGCTCAGTACGAGTGGCGATTGCCGCACTTAATATGCGCTCATCGGCATACAGCGACAGGCGGCGACGCGCGCGGGTCACCGCGGTATAAACCAGCtctc < 1:781929/140‑1 (MQ=255) ttATTCCCGTGAACTAAACAACGCCGCCAGACCACTGCGCCGCTCAGTACGAGTGGCGATTGCCGCACTTAATATGCGCTCATCGGCATACAGCGACAGGCGGCGACGCGCGCGGGTCACCGCGGTATAAACCAGCtctc < 1:476082/140‑1 (MQ=255) cccGTGAACTAAACAACGCCGCCAGACCACTGCGCCGCTCAGTACGAGTGGCGATTGCCGCACTTAATATGCGCTCATCGGCATACAGCGACAGGCGGCGACGCGCGCGGGTCACCGCGGTATAAACCAGCTCTCGCGtt > 1:799509/1‑140 (MQ=255) gTGAACTAAACAACGCCGCCAGACCACTGCGCCGCTCAGTACGAGTGGCGATTGCCGCACTTAATATGCGCTCATCGGCATACAGCGACAGGCGGCGACGCGCGCGGGTCACCGCGGTATAAACCAGCTCTCGCGTtact > 1:917119/1‑140 (MQ=255) gAACTAAACAACGCCGCCAGACCACTGCGCCGCTCAGTACGAGTGGCGATTGCCGCACTTAATATGCGCTCATCGGCATACAGCGACAGGCGGCGACGCGCGCGGGTCACCGCGGTATAAACCAGCTCTCGCGTtactac > 2:295360/1‑140 (MQ=255) gAACTAAACAACGCCGCCAGACCACTGCGCCGCTCAGTACGAGTGGCGATTGCCGCACTTAATATGCGCTCATCGGCATACAGCGACAGGCGGCGACGCGCGCGGGTCACCGCGGTATAAACCAGCTCTCGCGTtactac < 1:876593/140‑1 (MQ=255) cgccgcCAGACCACTGCGCCGCTCAGTACGAGTGGCGATTGCCGCACTTAATATGCGCTCATCGGCATACAGCGACAGGCGGCGACGCGCGCGGGTCACCGCGGTATAAACCAGCTCTCGCGTTACTACCGGCGTGCGtt > 1:607789/1‑140 (MQ=255) ccgccAGACCACTGCGCCGCTCAGTACGAGTGGCGATTGCCGCACTTAATATGCGCTCATCGGCATACAGCGACAGGCGGCGACGCGCGCGGGTCACCGCGGTATAAACCAGCTCTCGCGTTACTACCGGCGTGCGTTgg > 2:203976/1‑140 (MQ=255) cgccAGACCACTGCGCCGCTCAGTACGAGTGGCGATTGCCGCACTTAATATGCGCTCATCGGCATACAGCGACAGGCGGCGACGCGCGCGGGTCACCGCGGTATAAACCAGCTCTCGCGTTACTACCGGCGTGCGTTGGc < 2:331071/140‑1 (MQ=255) gccAGACCACTGCGCCGCTCAGTACGAGTGGCGATTGCCGCACTTAATATGCGCTCATCGGCATACAGCGACAGGCGGCGACGCGCGCGGGTCACCGCGGTATAAACCAGCTCTCGCGTTACTACCGGCGTGCGTTGGCt < 1:197995/140‑1 (MQ=255) cTGCGCCGCTCAGTACGAGTGGCGATTGCCGCACTTAATATGCGCTCATCGGCATACAGCGACAGGCGGCGACGCGCGCGGGTCACCGCGGTATAAACCAGCTCTCGCGTTACTACCGGCGTGCGTTGGCTCGGCAAAAt > 1:50720/1‑140 (MQ=255) ccgcTCAGTACGAGTGGCGATTGCCGCACTTAATATGCGCTCATCGGCATACAGCGACAGGCGGCGACGCGCGCGGGTCACCGCGGTATAAACCAGCTCTCGCGTTACTACCGGCGTGCGTTGGCTCGGCAAAATCAAcg > 2:604994/1‑140 (MQ=255) cTCAGTACGAGTGGCGATTGCCGCACTTAATATGCGCTCATCGGCATACAGCGACAGGCGGCGACGCGCGCGGGTCACCGCGGTATAAACCAGCTCTCGCGTTACTACCGGCGTGCGTTGGCTCGGCAAAATCAAcgccg > 1:299586/1‑140 (MQ=255) aGTACGAGTGGCGATTGCCGCACTTAATATGCGCTCATCGGCATACAGCGACAGGCGGCGACGCGCGCGGGTCACCGCGGTATAAACCAGCTCTCGCGTTACTACCGGCGTGCGTTGGCTCGGCAAAATCAACGCCGCAt > 2:158469/1‑140 (MQ=255) cGAGTGGCGATTGCCGCACTTAATATGCGCTCATCGGCATACAGCGACAGGCGGCGACGCGCGCGGGTCACCGCGGTATAAACCAGCTCTCGCGTTACTACCGGCGTGCGTTGGCTCGGCAAAATCAACGCCGCATGGTc < 2:279078/140‑1 (MQ=255) cGAGTGGCGATTGCCGCACTTAATATGCGCTCATCGGCATACAGCGACAGGCGGCGACGCGCGCGGGTCACCGCGGTATAAACCAGCTCTCGCGTTACTACCGGCGTGCGTTGGCTCGGCAAAATCAACGCCGCATGGTc < 2:829129/140‑1 (MQ=255) cGAGTGGCGATTGCCGCACTTAATATGCGCTCATCGGCATACAGCGACAGGCGGCGACGCGCGCGGGTCACCGCGGTATAAACCAGCTCTCGCGTTACTACCGGCGTGCGTTGGCTCGGCAAAATCAACGCCGCATGGTc < 1:431593/140‑1 (MQ=255) cGAGTGGCGATTGCCGCACTTAATATGCGCTCATCGGCATACAGCGACAGGCGGCGACGCGCGCGGGTCACCGCGGTATAAACCAGCTCTCGCGTTACTACCGGCGTGCGTTGGCTCGGCAAAATCAACGCCGCATGGTc < 1:604994/140‑1 (MQ=255) aGTGGCGATTGCCGCACTTAATATGCGCTCATCGGCATACAGCGACAGGCGGCGACGCGCGCGGGTCACCGCGGTATAAACCAGCTCTCGCGTTACTACCGGCGTGCGTTGGCTCGGCAAAATCAACGCCGCATGGTCGa < 2:917119/140‑1 (MQ=255) gATTGCCGCACTTAATATGCGCTCATCGGCATACAGCGACAGGCGGCGACGCGCGCGGGTCACCGCGGTATAAACCAGCTCTCGCGTTACTACCGGCGTGCGTTGGCTCGGCAAAATCAACGCCGCATGGTCGAACTCCg > 2:59155/1‑140 (MQ=255) gATTGCCGCACTTAATATGCGCTCATCGGCATACAGCGACAGGCGGCGACGCGCGCGGGTCACCGCGGTATAAACCAGCTCTCGCGTTACTACCGGCGTGCGTTGGCTCGGCAAAATCAACGCCGCATGGTCGAACTCCg > 2:694066/1‑140 (MQ=255) ccGCACTTAATATGCGCTCATCGGCATACAGCGACAGGCGGCGACGCGCGCGGGTCACCGCGGTATAAACCAGCTCTCGCGTTACTACCGGCGTGCGTTGGCTCGGCAAAATCAACGCCGCATGGTCGAACTCCGATccc < 2:299586/140‑1 (MQ=255) cACTTAATATGCGCTCATCGGCATACAGCGACAGGCGGCGACGCGCGCGGGTCACCGCGGTATAAACCAGCTCTCGCGTTACTACCGGCGTGCGTTGGCTCGGCAAAATCAACGCCGCATGGTCGAACTCCGATCCCTGc < 1:694066/140‑1 (MQ=255) cTTAATATGCGCTCATCGGCATACAGCGACAGGCGGCGACGCGCGCGGGTCACCGCGGTATAAACCAGCTCTCGCGTTACTACCGGCGTGCGTTGGCTCGGCAAAATCAACGCCGCATGGTCGAACTCCGATCCCTGCGa < 2:50720/140‑1 (MQ=255) aaTATGCGCTCATCGGCATACAGCGACAGGCGGCGACGCGCGCGGGTCACCGCGGTATAAACCAGCTCTCGCGTTACTACCGGCGTGCGTTGGCTCGGCAAAATCAACGCCGCATGGTCGAACTc > 1:610303/1‑125 (MQ=255) aaTATGCGCTCATCGGCATACAGCGACAGGCGGCGACGCGCGCGGGTCACCGCGGTATAAACCAGCTCTCGCGTTACTACCGGCGTGCGTTGGCTCGGCAAAATCAACGCCGCATGGTCGAACTc < 2:610303/125‑1 (MQ=255) cTCATCGGCATACAGCGACAGGCGGCGACGCGCGCGGGTCACCGCGGTATAAACCAGCTCTCGCGTTACTACCGGCGTGCGTTGGCTCGGCAAAATCAACGCCGCATGGTCGAACTCCGATCCCTGCGATTTATGTACCg > 1:707559/1‑140 (MQ=255) cTCATCGGCATACAGCGACAGACGGCGACGCGCGCGGGTCACCGCGGTATAAACCAGCTCTCGCGTTACTACCGGCGTGCGTTGGCTCGGCAAAATCAACGCCGCATGGTCGAACTCCGATCCCTGCGATTTATGTACCg > 2:361327/1‑140 (MQ=255) cAGCGACAGGCGGCGACGCGCGCGGGTCACCGCGGTATAAACCAGCTCTCGCGTTACTACCGGCGTGCGTTGGCTCGGCAAAATCAACGCCGCATGGTCGAACTCCGATCCCTGCGATTTATGTACCGTCATCGCCCACg < 2:607789/140‑1 (MQ=255) cAGCGACAGGCGGCGACGCGCGCGGGTCACCGCGGTATAAACCAGCTCTCGCGTTACTACCGGCGTGCGTTGGCTCGGCAAAATCAACGCCGCATGGTCGAACTCCGATCCCTGCGATTTATGTACCGTCATCGCCCACg < 1:227007/140‑1 (MQ=255) aGCGACAGGCGGCGACGCGCGCGGGTCACCGCGGTATAAACCAGCTCTCGCGTTACTACCGGCGTGCGTTGGCTCGGCAAAATCAACGCCGCATGGTCGAACTCCGATCCCTGCGATTTATGTa < 2:353082/124‑1 (MQ=255) aGCGACAGGCGGCGACGCGCGCGGGTCACCGCGGTATAAACCAGCTCTCGCGTTACTACCGGCGTGCGTTGGCTCGGCAAAATCAACGCCGCATGGTCGAACTCCGATCCCTGCGATTTATGTa > 1:353082/1‑124 (MQ=255) aGCGACAGGCGGCGACGCGCGCGGGTCACCGCGGTATAAACCAGCTCTCGCGTTACTACCGGCGTGCGTTGGCTCGGCAAAATCAACGCCGCATGGTCGAACTCCGATCCCTGCGATTTATGTACCGTCATCGCCCACGt > 1:6709/1‑140 (MQ=255) aGCGACAGGCGGCGACGCGCGCGGGTCACCGCGGTATAAACCAGCTCTCGCGTTACTACCGGCGTGCGTTGGCTCGGCAAAATCAACGCCGCATGGTCGAACTCCGATCCCTGCGATTTATGTACCGTCATCGCCCACGt > 1:328895/1‑140 (MQ=255) | CGTAACACTCGTACGTCGCATCCGGCAATTACGTTTATTCCCGTGAACTAAACAACGCCGCCAGACCACTGCGCCGCTCAGTACGAGTGGCGATTGCCGCACTTAATATGCGCTCATCGGCATACAGCGACAGACGGCGACGCGCGCGGGTCACCGCGGTATAAACCAGCTCTCGCGTTACTACCGGCGTGCGTTGGCTCGGCAAAATCAACGCCGCATGGTCGAACTCCGATCCCTGCGATTTATGTACCGTCATCGCCCACGT > NC_000913/2950601‑2950865 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |