Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,851,687	A→T	D366E (GAT→GAA)	ilvB ←	acetohydroxy acid synthase I subunit IlvB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,851,687	0	A	T	100.0%	216.4 / NA	62	D366E (GAT→GAA)	ilvB	acetohydroxy acid synthase I subunit IlvB
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (39/23);  total (39/23)

GTCAGCCACTGGCGTGGGCGATTGAGCGGATAAGCTTGCGCGGTCCACATCTGATGCTGACCAACGTCGGTGGTGATAATTGCATTGTCATCGACACAGGCGGCAACGGCGTTGATCAGGCCGTAATGGCTTAACGGATCGCACGCTTTCGGGATTGGACACGGAAACTCACGCTGCAAATCCGCTACCAACTGGTGCCACTCTGCACGCGGTTGCGCTTCCACCAGCGGGATCAACTGCGCCAGCACGTCATCAACATCCGCCTGAATC  >  NC_000913/3851550‑3851819                                                                                                                                          |                                                                                                                                     gTCAGCCACTGGCGTGGGCGATTGAGCGGATAAGCTTGCGCGGTCCACATCTGATGCTGACCAACGTCGGTGGTGATAATTGCATTGTCATCGACACAGGCGGCAACGGCGTTGATCAGGCCGTAATGGCTTAACGGTTc                                                                                                                                    >  2:35971/1‑140 (MQ=255)    aGCCACTGGCGTGGGCGATTGAGCGGATAAGCTTGCGCGGTCCACATCTGATGCTGACCAACGTCGGTGGTGATAATTGCATTGTCATCGACACAGGCGGCAACGGCGTTGATCAGGCCGTAATGGCTTAACGGTTCGCa                                                                                                                                 <  1:411096/140‑1 (MQ=255)    aGCCACTGGCGTGGGCGATTGAGCGGATAAGCTTGCGCGGTCCACATCTGATACTGACCAACGTCGGTGGTGATAATTGCATTGTCATCGACACAGGCGGCAACGGCGTTGATCAGGCCGTAATGGCTTAACGGTTCGCa                                                                                                                                 <  1:46697/140‑1 (MQ=255)         cTGGCGTGGGCGATTGAGCGGATAAGCTTGCGCGGTCCACATCTGATGCTGACCAACGTCGGTGGTGATAATTGCATTGTCATCGACACAGGCGGCAACGGCGTTGATCAGGCCGTAATGGCTTAACGGTTCGCACGCtt                                                                                                                            >  1:806376/1‑140 (MQ=255)              gTGGGCGATTGAGCGGATAAGCTTGCGCGGTCCACATCTGATGCTGACCAACGTCGGTGGTGATAATTGCATTGTCATCGACACAGGCGGCAACGGCGTTGATCAGGCCGTAATGGCTTAACGGTTCGCACGCTTTCggg                                                                                                                       >  2:39316/1‑140 (MQ=255)                      ttGAGCGGATAAGCTTGCGCGGTCCACATCTGATGCTGACCAACGTCGGTGGTGATAATTGCATTGTCATCGACACAGGCGGCAACGGCGTTGATCAGGCCGTAATGGCTTAACGGTTCGCACGCTTTCGGGATTGGaca                                                                                                               >  1:833142/1‑140 (MQ=255)                           cGGATAAGCTTGCGCGGTCCACATCTGATGCTGACCAACGTCGGTGGTGATAATTGCATTGTCATCGACACAGGCGGCAACGGCGTTGATCAGGCCGTAATGGCTTAACGGTTCGCACGCTTTCGGGATTGGACACGGaa                                                                                                          >  2:79364/1‑140 (MQ=255)                                 aGCTTGCGCGGTCCACATCTGATGCTGACCAACGTCGGTGGTGATAATTGCATTGTCATCGACACAGGCGGCAACGGCGTTGATCAGGCCGTAATGGCTTAACGGTTCGCACGCTTTCGGGATTGGACACGGAAACTCAc                                                                                                    <  1:627698/140‑1 (MQ=255)                                 aGCTTGCGCGGTCCACATCTGATGCTGACCAACGTCGGTGGTGATAATTGCATTGTCATCGACACAGGCGGCAACGGCGTTGATCAGGCCGTAATGGCTTAACGGTTCGCACGCTTTCGGGATTGGACACGGAAACTCAc                                                                                                    <  2:711456/140‑1 (MQ=255)                                     tGCGCGGTCCACATCTGATGCTGACCAACGTCGGTGGTGATAATTGCATTGTCATCGACACAGGCGGCAACGGCGTTGATCAGGCCGTAATGGCTTAACGGTTCGCACGCTTTCGGGATTGGACACGGAAACTCACGCTg                                                                                                >  2:434137/1‑140 (MQ=255)                                      gcgcGGTCCACATCTGATGCTGACCAACGTCGGTGGTGATAATTGCATTGTCATCGACACAGGCGGCAACGGCGTTGATCAGGCCGTAATGGCTTAACGGTTCGCACGCTTTCGGGATTGGa                                                                                                                 >  1:610186/1‑122 (MQ=255)                                      gcgcGGTCCACATCTGATGCTGACCAACGTCGGTGGTGATAATTGCATTGTCATCGACACAGGCGGCAACGGCGTTGATCAGGCCGTAATGGCTTAACGGTTCGCACGCTTTCGGGATTGGa                                                                                                                 <  2:610186/122‑1 (MQ=255)                                       cgcgGTCCACATCTGATGCTGACCAACGTCGGTGGTGATAATTGCATTGTCATCGACACAGGCGGCAACGGCGTTGATCAGGCCGTAATGGCTTAACGGTTCGCACGCTTTCGGGATTGGACACGGAAACTCACGCTGCa                                                                                              >  1:679069/1‑140 (MQ=255)                                                      aTGCTGACCAACGTCGGTGGTGATAATTGCATTGTCATCGACACAGGCGGCAACGGCGTTGATCAGGCCGTAATGGCTTAACGGTTCGCACGCTTTCGGGATTGGACACGGAAACTCACGCTGCAAATCCGCTACCAACt                                                                               >  1:550763/1‑140 (MQ=255)                                                      aTGCTGACCAACGTCGGTGGTGATAATTGCATTGTCATCGACACAGGCGGCAACGGCGTTGATCAGGCCGTAATGGCTTAACGGTTCGCACGCTTTCGGGATTGGACACGGAAACTCACGCTGCAAATCCGCTACCAACt                                                                               >  1:851240/1‑140 (MQ=255)                                                          tGACCAACGTCGGTGGTGATAATTGCATTGTCATCGACACAGGCGGCAACGGCGTTGATCAGGCCGTAATGGCTTAACGGTTCGCACGCTTTCGGGATTGGACACGGAAACTCACGCTGCAAATCCGCTACCAACTGGTg                                                                           >  2:52838/1‑140 (MQ=255)                                                           gACCAACGTCGGTGGTGATAATTGCATTGTCATCGACACAGGCGGCAACGGCGTTGATCAGGCCGTAATGGCTTAACGGTTCGCACGCTTTCGGGATTGGACACGGAAACTCACGCTGCAAATCCGCTACCAAc                                                                                <  2:130420/134‑1 (MQ=255)                                                           gACCAACGTCGGTGGTGATAATTGCATTGTCATCGACACAGGCGGCAACGGCGTTGATCAGGCCGTAATGGCTTAACGGTTCGCACGCTTTCGGGATTGGACACGGAAACTCACGCTGCAAATCCGCTACCAAc                                                                                >  1:130420/1‑134 (MQ=255)                                                           gACCAACGTCGGTGGTGATAATTGCATTGTCATCGACACAGGCGGCAACGGCGTTGATCAGGCCGTAATGGCTTAACGGTTCGCACGCTTTCGGGATTGGACACGGAAACTCACGCTGCAAATCCGCTACCAACTGGTGc                                                                          >  2:450220/1‑140 (MQ=255)                                                           gACCAACGTCGGTGGTGATAATTGCATTGTCATCGACACAGGCGGCAACGGCGTTGATCAGGCCGTAATGGCTTAACGGTTCGCACGCTTTCGGGATTGGACACGGAAACTCACGCTGCAAATCCGCTACCAACTGGTGc                                                                          <  2:851240/140‑1 (MQ=255)                                                           gACCAACGTCGGTGGTGATAATTGCATTGTCATCGACACAGGCGGCAACGGCGTTGATCAGGCCGTAATGGCTTAACGGTTCGCACGCTTTCGGGATTGGACACGGAAACTCACGCTGCAAATCCGCTACCAACTGGTGc                                                                          >  2:921042/1‑140 (MQ=255)                                                           gACCAACGTCGGTGGTGATAATTGCATTGTAATCGACACAGGCGGCAACGGCGTTGATCAGGCCGTAATGGCTTAACGGTTCGCACGCTTTCGGGATTGGACACGGAAACTCACGCTGCAAATCCGCTa                                                                                     <  2:30687/129‑1 (MQ=255)                                                           gACCAACGTCGGTGGTGATAATTGCATTGTAATCGACACAGGCGGCAACGGCGTTGATCAGGCCGTAATGGCTTAACGGTTCGCACGCTTTCGGGATTGGACACGGAAACTCACGCTGCAAATCCGCTa                                                                                     >  1:30687/1‑129 (MQ=255)                                                             ccAACGTCGGTGGTGATAATTGCATTGTCATCGACACAGGCGGCAACGGCGTTGATCAGGCCGTAATGGCTTAACGGTTCGCACGCTTTCGGGATTGGACACGGAAACTCACGCTGCAAATCCGCTACCAACTGGTGCCa                                                                        <  1:79364/140‑1 (MQ=255)                                                                 cGTCGGTGGTGATAATTGCATTGTCATCGACACAGGCGGCAACGGCGTTGATCAGGCCGTAATGGCTTAACGGTTCGCACGCTTTCGGGATTGGACACGGAAACTCACGCTGCAAATCCGCTACCa                                                                                  <  2:894411/126‑1 (MQ=255)                                                                 cGTCGGTGGTGATAATTGCATTGTCATCGACACAGGCGGCAACGGCGTTGATCAGGCCGTAATGGCTTAACGGTTCGCACGCTTTCGGGATTGGACACGGAAACTCACGCTGCAAATCCGCTACCa                                                                                  >  1:894411/1‑126 (MQ=255)                                                                 cGTCGGTGGTGATAATTGCATTGTCATCGACACAGGCGGCAACGGCGTTGATCAGGCCGTAATGGCTTAACGGTTCGCACGCTTTCGGGATTGGACACGGAAACTCACGCTGCAAATCCGCTACCAACTGGTGCCActct                                                                    >  2:855739/1‑140 (MQ=255)                                                                      gtggtgATAATTGCATTGTCATCGACACAGGCGGCAACGGCGTTGATCAGGCCGTAATGGCTTAACGGTTCGCACGCTTTCGGGATTGGACACGGAAACTCACGCTGCAAATCCGCTACCAACTGGTGCCACTCTGCAcg                                                               >  1:153036/1‑140 (MQ=255)                                                                              aaTTGCATTGTCATCGACACAGGCGGCAACGGCGTTGATCAGGCCGTAATGGCTTAACGGTTCGCACGCTTTCGGGATTGGACACGGAAACTCACGCTGCAAATCCGCTACCAACTGGTGCCACTCTGCACGCGGTTgcg                                                       >  1:733203/1‑140 (MQ=255)                                                                                 tGCATTGTCATCGACACAGGCGGCAACGGCGTTGATCAGGCCGTAATGGCTTAACGGTTCGCACGCTTTCGGGATTGGACACGGAAACTCACGCTGCAAATCCGCTACCAACTGGTGCCACTCTGCACGCGGTTGCGCtt                                                    >  1:460370/1‑140 (MQ=255)                                                                                   cATTGTCATCGACACAGGCGGCAACGGCGTTGATCAGGCCGTAATGGCTTAACGGTTCGCACGCTTTCGGGATTGGACACGGAAACTCACGCTGCAAATCCGCTACCAACTGGTGCCACTCTGCACGCGGTTGCGCTTcc                                                  >  1:638337/1‑140 (MQ=255)                                                                                    aTTGTCATCGACACAGGCGGCAACGGCGTTGATCAGGCCGTAATGGCTTAACGGTTCGCACGCTTTCGGGATTGGACACGGAAACTCACGCTGCAAATCCGCTACCAACTGGTGCCACTCTGCACGCGGTTGCGCTTcca                                                 >  1:691671/1‑140 (MQ=255)                                                                                       gTCATCGACACAGGCGGCAACGGCGTTGATCAGGCCGTAATGGCTTAACGGTTCGCACGCTTTCGGGATTGGACACGGAAACTCACGCTGCAAATCCGCTACCAACTGGTGCCACTCTGCACGCGGTTGCGCTTccacca                                              <  2:153036/140‑1 (MQ=255)                                                                                       gTCATCGACACAGGCGGCAACGGCGTTGATCAGGCCGTAATGGCTTAACGGTTCGCACGCTTTCGGGATTGGACACGGAAACTCACGCTGCAAATCCGCTACCAACTGGTGCCACTCTGCACGCGGTTGCGCTTccacca                                              <  1:660330/140‑1 (MQ=255)                                                                                           tCGACACAGGCGGCAACGGCGTTGATCAGGCCGTAATGGCTTAACGGTTCGCACGCTTTCGGGATTGGACACGGAAACTCACGCTGCAAATCCGCTACCAACTGGTGCCACTCTGCACGCGGTTGCGCTTccacca                                              >  1:867382/1‑136 (MQ=255)                                                                                           tCGACACAGGCGGCAACGGCGTTGATCAGGCCGTAATGGCTTAACGGTTCGCACGCTTTCGGGATTGGACACGGAAACTCACGCTGCAAATCCGCTACCAACTGGTGCCACTCTGCACGCGGTTGCGCTTccacca                                              <  2:867382/136‑1 (MQ=255)                                                                                                 caGGCGGCAACGGCGTTGATCAGGCCGTAATGGCTTAACGGTTCGCACGCTTTCGGGATTGGACACGGAAACTCACGCTGCAAATCCGCTACCAACTGGTGCCACTCTGCACGCGGTTGCGCTTCCACCAGCGGGATCaa                                    <  2:733203/140‑1 (MQ=255)                                                                                                  aGGCGGCAACGGCGTTGATCAGGCCGTAATGGCTTAACGGTTCGCACGCTTTCGGGATTGGACACGGAAACTCACGCTGCAAATCCGCTACCAACTGGTGCCACTCTGCACGCGGTTGCGCTTCCACCAGCGGGATCAAc                                   <  1:81031/140‑1 (MQ=255)                                                                                                  aGGCGGCAACGGCGTTGATCAGGCCGTAATGGCTTAACGGTTCGCACGCTTTCGGGATTGGACACGGAAACTCACGCTGCAAATCCGCTACCAACTGGTGCCACTCTGCACGCGGTTGCGCTTCCACCAGCGGGATCAAc                                   >  2:203958/1‑140 (MQ=255)                                                                                                  aGGCGGCAACGGCGTTGATCAGGCCGTAATGGCTTAACGGTTCGCACGCTTTCGGGATTGGACACGGAAACTCACGCTGCAAATCCGCTACCAACTGGTGCCACTCTGCACGCGGTTGCGCTTCCACCAGCGGGATCAAc                                   >  2:170964/1‑140 (MQ=255)                                                                                                  aGGCGGCAACGGCGTTGATCAGGCCGTAATGGCTTAACGGTTCGCACGCTTTCGGGATTGGACACGGAAACTCACGCTGCAAATCCGCTACCAACTGGTGCCACTCTGCACGCGGTTGCGCTTCCACCAGCGGGATCAAc                                   >  2:81031/1‑140 (MQ=255)                                                                                                    gcggcAACGGCGTTGATCAGGCCGTAATGGCTTAACGGTTCGCACGCTTTCGGGATTGGACACGGAAACTCACGCTGCAAATCCGCTACCAACTGGTGCCACTCTGCACGCGGTTGCGCTTCCACCAg                                             >  2:453824/1‑128 (MQ=255)                                                                                                    gcggcAACGGCGTTGATCAGGCCGTAATGGCTTAACGGTTCGCACGCTTTCGGGATTGGACACGGAAACTCACGCTGCAAATCCGCTACCAACTGGTGCCACTCTGCACGCGGTTGCGCTTCCACCAg                                             <  1:453824/128‑1 (MQ=255)                                                                                                    gcggcAACGGCGTTGATCAGGCCGTAATGGCTTAACGGTTCGCACGCTTTCGGGATTGGACACGGAAACTCACGCTGCAAATCCGCTACCAACTGGTGCCACTCTGCACGCGGTTGCGCTTCCACCAGCGGGATCAACTg                                 >  2:518933/1‑140 (MQ=255)                                                                                                             gCGTTGATCAGGCCGTAATGGCTTAACGGTTCGCACGCTTTCGGGATTGGACACGGAAACTCACGCTGCAAATCCGCTACCAACTGGTGCCACTCTGCACGCGGTTGCGCTTCCACCAGCGGGATCAACTGCGCCAGCAc                        <  2:638337/140‑1 (MQ=255)                                                                                                              cGTTGATCAGGCCGTAATGGCTTAACGGTTCGCACGCTTTCGGGATTGGACACGGAAACTCACGCTGCAAATCCGCTACCAACTGGTGCCACTCTGCACGCGGTTGCGCTTCCACCAGCGGGATCAACTGCGCCAGCACg                       >  1:7693/1‑140 (MQ=255)                                                                                                              cGTTGATCAGGCCGTAATGGCTTAACGGTTCGCACGCTTTCGGGATTGGACACGGAAACTCACGCTGCAAATCCGCTACCAACTGGTGCCACTCTGCACGCGGTTGCGCTTCCACCAGCGGGATCAACTGCGCCAGCACg                       <  2:691671/140‑1 (MQ=255)                                                                                                              cGTTGATCAGGCCGTAATGGCTTAACGGTTCGCACGCTTTCGGGATTGGACACGGAAACTCACGCTGCAAATCCGCTACCAACTGGTGCCACTCTGCACGCGGTTGCGCTTCCACCAGCGGGATCAACTGCGCCAGCACg                       >  2:541057/1‑140 (MQ=255)                                                                                                              cGTTGATCAGGCCGTAATGGCTTAACGGTTCGCACGCTTTCGGGATTGGACACGGAAACTCACGCTGCAAATCCGCTACCAACTGGTGCCACTCTGCACGCGGTTGCGCTTCCACCAGCGGGATCAACTGCGCCAGCACg                       >  2:171765/1‑140 (MQ=255)                                                                                                              cGTTGATCAGGCCGTAATGGCTTAACGGTTCGCACGCTTTCGGGATTGGACACGGAAACTCACGCTGCAAATCCGCTACCAACTGGTGCCACTCTGCACGCGGTTGCGCTTCCACCAGCGGGATCAACTGCGCCAGCACg                       >  2:357964/1‑140 (MQ=255)                                                                                                                       ggCCGTAATGGCTTAACGGTTCGCACGCTTTCGGGATTGGACACGGAAACTCACGCTGCAAATCCGCTACCAACTGGTGCCACTCTGCACGCGGTTGCGCTTCCACCAGCGGGATCAACTGCGCCAGCACGTCATCAACa              <  1:52838/140‑1 (MQ=255)                                                                                                                       ggCCGTAATGGCTTAACGGTTCGCACGCTTTCGGGATTGGACACGGAAACTCACGCTGCAAATCCGCTACCAACTGGTGCCACTCTGCACGCGGTTGCGCTTCCACCAGCGGGATCAACTGCGCCAGCACGTCATCAACa              <  2:7693/140‑1 (MQ=255)                                                                                                                        gCCGTAATGGCTTAACGGTTCGCACGCTTTCGGGATTGGACACGGAAACTCACGCTGCAAATCCGCTACCAACTGGTGCCACTCTGCACGCGGTTGCGCTTCCACCAGCGGGATCAACTGCGCCAGCa                         >  1:384153/1‑128 (MQ=255)                                                                                                                        gCCGTAATGGCTTAACGGTTCGCACGCTTTCGGGATTGGACACGGAAACTCACGCTGCAAATCCGCTACCAACTGGTGCCACTCTGCACGCGGTTGCGCTTCCACCAGCGGGATCAACTGCGCCAGCa                         <  2:384153/128‑1 (MQ=255)                                                                                                                        gCCGTAATGGCTTAACGGTTCGCACGCTTTCGGGATTGGACACGGAAACTCACGCTGCAAATCCGCTACCAACTGGTGCCACTCTGCACGCGGTTGCGCTTCCACCAGCGGGATCAACTGCGCCAGCACGTCATCAACAt             <  1:35971/140‑1 (MQ=255)                                                                                                                        gCCGTAATGGCTTAACGGTTCGCACGCTTTCGGGATTGGACACGGAAACTCACGCTGCAAATCCGCTACCAACTGGTGCCACTCTGCACGCGGTTGCGCTTCCACCAGCGGGATCAACTGCGCCAGCACGTCATCAACAt             >  2:478118/1‑140 (MQ=255)                                                                                                                        gCCGTAATGGCTTAACGGTTCGCACGCTTTCGGGATTGGACACGGAAACTCACGCTGCAAATCCGCTACCAACTGGTGCCACTCTGCACGCGGTTGCACTTCCACCAGCGGGATCAACTGCGCCAGCACGTCATCAACAt             >  2:568455/1‑140 (MQ=255)                                                                                                                          cGTAATGGCTTAACGGTTCGCACGCTTTCGGGATTGGACACGGAAACTCACGCTGCAAATCCGCTACCAACTGGTGCCACTCTGCACGCGGTTGCGCTTCCACCAGCGGGATCAACTGCGCCAGCACGTCATCAACATcc           <  2:137931/140‑1 (MQ=255)                                                                                                                             aaTGGCTTAACGGTTCGCACGCTTTCGGGATTGGACACGGAAACTCACGCTGCAAATCCGCTACCAACTGGTGCCACTCTGCACGCGGTTGCGCTTCCACCAGCGGGATCAACTGCGCCAGCACGTCATCAACATCCGcc        >  2:499373/1‑140 (MQ=255)                                                                                                                                  cTTAACGGTTCGCACGCTTTCGGGATTGGACACGGAAACTCACGCTGCAAATCCGCTACCAACTGGTGCCACTCTGCACGCGGTTGCGCTTCCACCAGCGGGATCAACTGCGCCAGCACGTCATCAACATCCGCCTGAAt   >  1:425248/1‑140 (MQ=255)                                                                                                                                  cTTAACGGTTCGCACGCTTTCGGGATTGGACACGGAAACTCACGCTGCAAATCCGCTACCAACTGGTGCCACTCTGCACGCGGTTGCGCTTCCACCAGCGGGATCAACTGCGCCAGCACGTCATCAACATCCGCCTGAAt   >  1:814566/1‑140 (MQ=255)                                                                                                                                   ttAACGGTTCGCACGCTTTCGGGATTGGACACGGAAACTCACGCTGCAAATCCGCTACCAACTGGTGCCACTCTGCACGCGGTTGCGCTTCCACCAGCTGGATCAACTGCGCCAGCACGTCATCAACATCCGCCTGAATc  >  1:58589/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                     GTCAGCCACTGGCGTGGGCGATTGAGCGGATAAGCTTGCGCGGTCCACATCTGATGCTGACCAACGTCGGTGGTGATAATTGCATTGTCATCGACACAGGCGGCAACGGCGTTGATCAGGCCGTAATGGCTTAACGGATCGCACGCTTTCGGGATTGGACACGGAAACTCACGCTGCAAATCCGCTACCAACTGGTGCCACTCTGCACGCGGTTGCGCTTCCACCAGCGGGATCAACTGCGCCAGCACGTCATCAACATCCGCCTGAATC  >  NC_000913/3851550‑3851819

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑