Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	445,558	0	A	C	32.0%	76.1 / 31.1	50	V370G (GTA→GGA)	yajR	putative major facilitator superfamily transport protein YajR
Reads supporting (aligned to +/- strand):  ref base A (26/8);  new base C (0/16);  total (26/24)
Fisher's exact test for biased strand distribution p-value = 1.49e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

AGCAAACGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTACTGATAAGTG  >  NC_000913/445420‑445693                                                                                                                                           |                                                                                                                                        aGCAAACGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTTATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAAAAc                                                                                                                                        >  1:995883/1‑140 (MQ=255) aGCAAACGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTTGCGACTGTCAGCCACACCGCGGCCAGCATTGCGCCAGCGAGAAATAc                                                                                                                                        >  1:391652/1‑140 (MQ=255) aGCAAACGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATAc                                                                                                                                        >  1:126899/1‑140 (MQ=255) aGCAAACGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATAc                                                                                                                                        >  1:533231/1‑140 (MQ=255)      aCGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCt                                                                                                                                   <  2:551340/140‑1 (MQ=255)      aCGCACTTTTAACCCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTTATGGGACTGGCGGCTGTCAGCCACACTCCGGGCAGCATTGCGCCAGCGGGAAAAAcccccc                                                                                                                                   >  2:14671/1‑139 (MQ=255)                   gCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACAt                                                                                                                      <  1:163642/140‑1 (MQ=255)                      tCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGcc                                                                                                                   >  1:216825/1‑140 (MQ=255)                        gTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTTATGGTACCGGCGACTGTCAGCCCCACCGCGGGCCGCATTGCGCCAGCGAGAAAAACCCCCCCACCCTCAAACATGCCgc                                                                                                                 >  2:1091397/1‑139 (MQ=255)                           tGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTaa                                                                                                              <  2:988296/140‑1 (MQ=255)                                cAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAg                                                                                                         <  1:838976/140‑1 (MQ=255)                                  aTGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTTATGGTACTGGCGACTGTCAGCCCCACTGCGGCCCGCATTGCGCCCGCGAGAAAAAACCCCCTACCGTCCAACATGCCGTTAATCCAgcc                                                                                                       >  2:119879/1‑140 (MQ=255)                                   tGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAgccg                                                                                                      >  2:564250/1‑140 (MQ=255)                                     ttCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATCCCCCCTGACCGTCAAACATGCCGTTAATCCAgccgcc                                                                                                    <  1:1091397/140‑1 (MQ=255)                                     ttCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTCGGGCCAGAATTGCGCCAGCGAGAAATCCCCCCTGACCGTAAAACATGCCGTTAATCCAgccgcc                                                                                                    <  2:985683/140‑1 (MQ=255)                                      tCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGccc                                                                                                   <  1:564250/140‑1 (MQ=255)                                       cccggggTTTTAAATGCGAAAACTGCTGACATGCGGCGTTTTTTTCAGGGTACGGGCGCCTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAAAAATCCCCCCTGCCCGTAAAACATGCCTTTAATCCAGCCGCCCa                                                                                                  <  2:961833/136‑1 (MQ=255)                                           gggTTTTCAAGGCGCAACCTGCTGACATACGGGGGTTTTTTCAGGTTCCGGGCGCCTGCCAGCCACCCGGCGGCCAGCTTGGCGCCAGCGAGAAATCCCCCCTGCCCGTAAAACATGCCGTTAATCCAGCCGCCCAGCGa                                                                                              <  2:1628046/140‑1 (MQ=255)                                             gATTTCAATGCGCAAACTGCTGACATACGGCGGTTTTTTTATTGGACTGGCGACTGTCAGCCACACTGCGGCCCGCATTTCGCCCGCGGGAAAAACCCCCCGACCCTCAAACATGCCGTTAATCCAGCCCCCCCCCGAAc                                                                                            >  2:1379268/1‑140 (MQ=255)                                             gATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAAc                                                                                            >  1:1628046/1‑140 (MQ=255)                                                 ccAATGCGAAAACTGCTGACATACGGCGTTTTTTTCAGGGTCTTGGCGACTGTCAGCCACACTGCGGCAAGCATGGCGCCAGCGAAAAATCCCCCCTGCCCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGcc                                                                                        <  2:1411147/139‑1 (MQ=255)                                                       cgcAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCCCACCGCGGCCCGCCTTGCGCCAGCGAGAAAAACCCCCCGACCGTCAAACATGCCGGTAATCCAGCCGCCCACCCAACCCCCCAAcgc                                                                                  >  2:202500/1‑140 (MQ=255)                                                            aCTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACCGCGGCCAGCATTGCGCCCGCGAGAAAAACCCCCTGACCGTCAAACAAGCCGTTAATCCACCCGCCCCGCGAACCGCCAATcgccccgc                                                                             >  2:973049/1‑140 (MQ=255)                                                             ctgctgACATCCGGGGTTTTTTCCATGGTCCGGGGGACTGCCACCCACCCGGGGGCCAGCATGGCCCCGGCGAAAAATCCCCCCTGCCCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATcgccacgcc                                                                            <  2:391652/140‑1 (MQ=255)                                                             ctgctgACATACGGCGGTTCTTTTATGGTACTGGCGACTGTCAACCACACTGCGGCCCGCAATTCGCCAGCGAGAAAAAACCCCCGACCGTCCAAAAAGCCGGTAATCCAGCCCCCCCGCGAACCGCCCATcgccacgcc                                                                            >  2:1732067/1‑140 (MQ=255)                                                                 tgACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCCGCATTGCGCCAGCGAGAAATAACCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCCCCCATCGCCACCCCAAGa                                                                        >  2:939452/1‑140 (MQ=255)                                                                               tCTTTCATGGTACTGGCGACTGTCAGCCACACCGCGGCCAGCATTTCGCCAGCGAGAAAAACCCCCTGACCGTCAAAAAAGCCGTTAATCCAGCCGCCCCGCCAACCGCCAAACGCCCCCCCAAAAAAATGGCtgggggg                                                          >  2:1577074/1‑139 (MQ=255)                                                                                   tCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATCCCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTaa                                                      <  1:14671/140‑1 (MQ=255)                                                                                           cTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCAt                                              >  2:192290/1‑140 (MQ=255)                                                                                            tGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATc                                             >  2:1181245/1‑140 (MQ=255)                                                                                            tGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATc                                             >  2:774454/1‑140 (MQ=255)                                                                                             ggCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAAAAATCCCCCCTGCCCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCg                                            <  1:282009/140‑1 (MQ=255)                                                                                             ggCGACTGCCAGCCACCCTGCGGCCAGCTTGGCCCCAGCGAAAAACCCCCCCGGCCGGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCg                                            <  2:1741112/140‑1 (MQ=255)                                                                                              gCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGc                                           >  1:1284477/1‑140 (MQ=255)                                                                                               cGACTGTCAGCCACCCTGCGGCCAGCATTGCGCCAGCGAGAAATCCCCCCTGCCCGCAAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGcc                                          <  1:202500/140‑1 (MQ=255)                                                                                                gccTGTGAGCCACACTGCGGCCAGCATTGCGCCAGCGAAAAATCCCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCg                                         <  1:1399175/138‑1 (MQ=255)                                                                                                gACTGTCAGCCACCCTGCGGCCAGCATTGCGCCGGCGAAAAATCCCCCCTGCCCGCAAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCg                                         <  2:643789/140‑1 (MQ=255)                                                                                                gACTGTCAGCCACACTGGGGCCAGCATTGCGCCAGCGAGAAATCCCCCCTGACCGTAAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCg                                         <  1:1294567/140‑1 (MQ=255)                                                                                                   tGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTAc                                      >  2:22207/1‑140 (MQ=255)                                                                                                   tGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTAc                                      >  1:663894/1‑140 (MQ=255)                                                                                                   tGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTAc                                      >  1:1620117/1‑140 (MQ=255)                                                                                                   tGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTAc                                      >  2:1545128/1‑140 (MQ=255)                                                                                                     tCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCt                                    >  1:45139/1‑140 (MQ=255)                                                                                                     tCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCt                                    >  1:1269032/1‑140 (MQ=255)                                                                                                     tCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGGGGAGGAAACACCCATCGCCGTACCt                                    >  2:1521175/1‑140 (MQ=255)                                                                                                        gCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTg                                 >  1:1741112/1‑140 (MQ=255)                                                                                                          cacaCTGCGGCCAGCATGGCCCCAGGGAAAAATCCCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTa                               <  2:166554/140‑1 (MQ=255)                                                                                                          cacaCTGCGGCAAGCATGGCCCCAGCGAAAAATCCCCCCTGCCGGTAAACCATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTa                               <  2:663894/140‑1 (MQ=255)                                                                                                            cacTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAAc                             >  2:1668025/1‑140 (MQ=255)                                                                                                               tGCGGCCGGAATTCCCCAAGCGAAAAACCCCCCCGACCCCCCAAACATCCCGTTAATCCAGCCGCCCAGCGATCCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACctg                          <  2:968680/140‑1 (MQ=255)                                                                                                               tGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACctg                          >  1:556240/1‑140 (MQ=255)                                                                                                               tGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACctg                          >  2:681572/1‑140 (MQ=255)                                                                                                               tGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACctg                          >  2:800200/1‑140 (MQ=255)                                                                                                                 cGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTTGAGTAAACACCCATCGCCGTACCTTTGTAACctgct                        >  1:399020/1‑140 (MQ=255)                                                                                                                   gCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTgg                      <  1:681572/140‑1 (MQ=255)                                                                                                                   gCCAGCATTGCCCCACCAAAAAATCCCCCCTGACCGCAAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTgg                      <  2:1715784/140‑1 (MQ=255)                                                                                                                   gCCAGCATGGCGCCAGCGAGAAATCCCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTgg                      <  1:1567118/140‑1 (MQ=255)                                                                                                                    ccAGCATTGCGCCGGCGAAAAATCCCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGc                     <  1:1732067/140‑1 (MQ=255)                                                                                                                    ccAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGc                     <  1:22207/140‑1 (MQ=255)                                                                                                                     cAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCg                    >  2:1186864/1‑140 (MQ=255)                                                                                                                      aGCATTGCGCCAGCGAGAAATCCCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGa                   <  1:774454/140‑1 (MQ=255)                                                                                                                      aGCATTGCGCCAGCGAGAAATCCCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGa                   <  1:192290/140‑1 (MQ=255)                                                                                                                      aGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGa                   <  1:800200/140‑1 (MQ=255)                                                                                                                      aGCATTGCGCCAGCGAAAAATCCCCCCTGACCGTAAAACATGCCGTTAACCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGa                   <  2:533231/140‑1 (MQ=255)                                                                                                                       gCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGAc                  >  1:1715784/1‑140 (MQ=255)                                                                                                                        ctttGCCCCAGCGAGAAATCCCCCCTGCCCGTAAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGAct                 <  2:179685/138‑1 (MQ=255)                                                                                                                        cATTGCGCCGGCGAGAAATCCCCCCTGACCGTAAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGAct                 <  1:705106/140‑1 (MQ=255)                                                                                                                        aaTTGCGCCAGCGAAAAATCCCCCCTGCCCGTAAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGAct                 <  1:883173/139‑1 (MQ=255)                                                                                                                          ttGCGCCAGCGAGAAATCCCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGActct               <  1:1181245/140‑1 (MQ=255)                                                                                                                            gcgcCAGCGAGAAATCCCCCTTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCttt             <  1:939452/140‑1 (MQ=255)                                                                                                                             cgcCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTa            >  1:179685/1‑140 (MQ=255)                                                                                                                             cgcCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTa            >  1:1439252/1‑140 (MQ=255)                                                                                                                             cgcCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTa            >  2:1065399/1‑140 (MQ=255)                                                                                                                             cgcCAGCGAAAAATACCCCCTGACCGACAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTa            <  1:1118266/140‑1 (MQ=255)                                                                                                                               ccAGCGAGAAATCCCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTACt          <  1:1379268/140‑1 (MQ=255)                                                                                                                                 ggCGAGAAATCCCCCCTGCCCGTAAACCATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTACTGa        <  2:216825/139‑1 (MQ=255)                                                                                                                                  gCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTACTGAt       >  2:324685/1‑140 (MQ=255)                                                                                                                                  gCGAAAAATCCCCCCTGCCCGTAAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTACTGAt       <  2:1439252/140‑1 (MQ=255)                                                                                                                                       aaaTCCCCCCTGACCGTAAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTACTGATAAGTg  <  1:973049/140‑1 (MQ=255)                                                                                                                                           |                                                                                                                                        AGCAAACGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTACTGATAAGTG  >  NC_000913/445420‑445693

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑