Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,101,514	0	G	T	22.2%	226.3 / 22.7	81	intergenic (+84/‑176)	sodA/kdgT	superoxide dismutase (Mn)/2‑dehydro‑3‑deoxy‑D‑gluconate:H(+) symporter
Reads supporting (aligned to +/- strand):  ref base G (15/48);  new base T (18/0);  total (33/48)
Fisher's exact test for biased strand distribution p-value = 2.27e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

TCTGGAACGTGGTGAACTGGGACGAAGCAGCGGCACGTTTTGCGGCGAAAAAATAATCATTTGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGG‑TGGTTTTTTTGTGATCAATTTCAAAATAAAAACAATGATCCGATAAAAATAAAACAGCGTTTCAATTGATGTGGTTTTGATCACTTTTATTGATTAAATGAATGTCTATCTTCGTTTCCATCAACACTGATGCTCCATTGAGGAATTA  >  NC_000913/4101375‑4101651                                                                                                                                             |                                                                                                                                          tCTGGAACGTGGTGAACTGGGACGAAGCAGCGGCACGTTTTGCGGCGAAAAAATAATCATTTGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGG‑TGGTTTTTTtg                                                                                                                                           <  2:1735822/140‑1 (MQ=255)   tGGAACGTGGTGAACTGGGACGAAGCAGCGGCACGTTTTGCGGCGAAAAAATAATCATTTGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGG‑TGGTTTTTTtgtg                                                                                                                                         >  1:944213/1‑140 (MQ=255)     gAACGTGGTGAACTGGGACGAAGCAGCGGCACGTTTTGCGGCGAAAAAATAATCATTTGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGG‑TGGGTTTTTTTTGAt                                                                                                                                       >  1:1685414/1‑140 (MQ=255)     gAACGTGGTGAACTGGGACGAAGCAGCGGCACGTTTTGCGGCGAAAAAATAATCATTTGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGG‑GGGGTTTTTtttggt                                                                                                                                       >  2:970011/1‑138 (MQ=255)        cGTGGTGAACTGGGACGAAGCAGCGGCACGTTTTGCGGCGAAAAAATAATCATTTGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGG‑TGGTTTTTTTGTGATCaa                                                                                                                                    >  2:1673524/1‑140 (MQ=255)          tggtgAACTGGGACGAAGCAGCGGCACGTTTTGCGGCGAAAAAATAATCATTTGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGG‑TGGGTTTTTTGTGATCAAtt                                                                                                                                  >  2:661216/1‑140 (MQ=255)            gtgAACTGGGACGAAGCAGCGGCACGTTTTGCGGCGAAAAAATAATCATTTGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGG‑TGGTTTTTTTGTGAt                                                                                                                                       <  2:394183/133‑1 (MQ=255)            gtgAACTGGGACGAAGCAGCGGCACGTTTTGCGGCGAAAAAATAATCATTTGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGG‑TGGGTTTTTTGTGAt                                                                                                                                       >  1:394183/1‑133 (MQ=255)               aaCTGGGACGAAGCAGCGGCACGTTTTGCGGCGAAAAAATAATCATTTGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGG‑TGGTTTTTTTTTGATCAAATTCaaa                                                                                                                             >  1:1328600/1‑140 (MQ=255)               aaCTGGGACGAAGCAGCGGCACGTTTTGCGGCGAAAAAATAATCATTTGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGG‑TGGTTTTTTTGTGATCAATTTCaaa                                                                                                                             <  1:531811/140‑1 (MQ=255)                 cTGGGACGAAGCAGCGGCACGTTTTGCGGCGAAAAAATAATCATTTGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGG‑TGGTTTTTTTGTGATCAATTTCAAAAt                                                                                                                           <  1:661216/140‑1 (MQ=255)                   gggACGAAGCAGCGGCACGTTTTGCGGCGAAAAAATAATCATTTGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGG‑TGGTTTTTTTGTGATCAATTTCAAAATaa                                                                                                                         <  2:657998/140‑1 (MQ=255)                    ggACGAAGCAGCGGCACGTTTTGCGGCGAAAAAATAATCATTTGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGG‑TGGTTTTTTTGTGATCAATTTCAAAATaaa                                                                                                                        >  1:775920/1‑140 (MQ=255)                    ggACGAAGCAGCGGCACGTTTTGCGGCGAAAAAATAATCATTTGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGG‑TGGTTTTTTTGTGATCAATTTCAAAATaaa                                                                                                                        <  1:892695/140‑1 (MQ=255)                    ggACGAAGCAGCGGCACGTTTTGCGGCGAAAAAATAATCATTTGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGG‑TGGGTTTTTTTTGGTCAATTTTAAAATaaa                                                                                                                        >  2:1554857/1‑140 (MQ=255)                    ggACGAAGCAGCGGCACGTTTTGCGGCGAAAAAATAATCATTTGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGG‑TGGGTTTTTTTTGATCAATTTTaaaaaaaa                                                                                                                        >  2:1098370/1‑140 (MQ=255)                     gACGAAGCAGCGGCACGTTTTGCGGCGAAAAAATAATCATTTGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGG‑TGGTTTTTTTGTGATCAATTTCAAAATaaaa                                                                                                                       <  1:745761/140‑1 (MQ=255)                     gACGAAGCAGCGGCACGTTTTGCGGCGAAAAAATAATCATTTGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGG‑TGGGTTTTTTTTGATCAATTTtaaaataaaa                                                                                                                       >  1:584275/1‑140 (MQ=255)                        gAAGCAGCGGCACGTTTTGCGGCGAAAAAATAATCATTTGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGG‑TGGTTTTTTTGTGATCAATTTCAAAATAAAAACa                                                                                                                    <  2:609126/140‑1 (MQ=255)                         aaGCAGCGGCACGTTTTGCGGCGAAAAAATAATCATTTGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGGTTGGTTTGTTTTTG‑TCAATTTTAAAATAAAAACaa                                                                                                                   >  2:622518/1‑140 (MQ=255)                         aaGCAGCGGCACGTTTTGCGGCGAAAAAATAATCATTTGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGG‑TGGTTTTTTTGTGATCAATTTCAAAAt                                                                                                                           <  2:188136/132‑1 (MQ=255)                         aaGCAGCGGCACGTTTTGCGGCGAAAAAATAATCATTTGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGG‑TGGGTTTTTTTTGATCAATTTTAaaaaaaaaaaaa                                                                                                                   >  2:421204/1‑140 (MQ=255)                         aaGCAGCGGCACGTTTTGCGGCGAAAAAATAATCATTTGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGG‑TGGGTTTTTTTTGATCAATTTCAaaaaaaaaaaaa                                                                                                                   >  1:954699/1‑140 (MQ=255)                         aaGCAGCGGCACGTTTTGCGGCGAAAAAATAATCATTTGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGG‑GGGTTTTTTTGTGATCAATTTCAAAAt                                                                                                                           >  1:188136/1‑132 (MQ=255)                                  cACGTTTTGCGGCGAAAAAATAATCATTTGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGG‑TGGTTTTTTTGTGATCAATTTCAAAATAAAAACAATGATCCGat                                                                                                          <  2:1035474/140‑1 (MQ=255)                                  cACGTTTTGCGGCGAAAAAATAATCATTTGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGG‑TGGTTTTTTTGTGATCAATTTCAAAATAAAAACAATGATCCGat                                                                                                          <  2:1174960/140‑1 (MQ=255)                                    cGTTTTGCGGCGAAAAAATAATCATTTGCCGCCTGCTGCAATGAGGCGTGTAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGG‑TGGTTTTTTTGTGATCAATTTCAAAATAAAAACAATGATCCGataa                                                                                                        <  2:310566/140‑1 (MQ=255)                                    cGTTTTGCGGCGAAAAAATAATCATTTGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGG‑TGGTTTTTTTGTGATCAATTTCAAAATAAAAACAATGATCCGataa                                                                                                        <  2:1326572/140‑1 (MQ=255)                                    cGTTTTGCGGCGAAAAAATAATCATTTGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGG‑TGGGTTTTTTTTGATCAATTTTAAAATAAAAAAAAAGATTCGataa                                                                                                        >  2:962491/1‑140 (MQ=255)                                            ggcgAAAAAATAATCATTTGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGG‑TGGCTTTTTTGTGATCAATTTCAAAATAAAAACAATGATCCGataaaaataaaa                                                                                                >  2:143345/1‑140 (MQ=255)                                                aaaaaaTAATCATTTGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGG‑TGGTTTTTTTTTGATCAATTTCAAAAAAAAAAAAAAGATCCGataaaaataaaaaaac                                                                                            >  2:1683752/1‑136 (MQ=255)                                                aaaaaaTAATCATTTGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGG‑GGGGTTTTTTTTGATAAATTTTACAATAAAAACCAAGATACCATAAAAAAAAAACaac                                                                                            >  2:1495180/1‑138 (MQ=255)                                                             ttGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGG‑TGGTTTTTTTGTGATCAATTTCAAAATAAAAACAATGATCCGATAAAAATAAAACAGCGTTTCAATTGAtg                                                                               <  2:858786/140‑1 (MQ=255)                                                             ttGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGG‑TGGTTTTTTTGTGATCAATTTCAAAATAAAAACAATGATCCGATAAAAATAAAACAGCGTTTCAATTGAtg                                                                               <  2:414422/140‑1 (MQ=255)                                                              tGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGG‑TGGTTTTTTTTTGATCAATTTTAAAATAAAAAAAATGATCCGATAAAAATAAAAAAGCGTTTCAATTGAtgt                                                                              >  1:120177/1‑140 (MQ=255)                                                                   ccTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGG‑TGGGTTTTTTTTGATTAATTTTAAAAAAAAAAAAAAGATCCGATAAAAAAAAAAAAACGTTTTAATTGATGTGGttt                                                                         >  2:1355816/1‑140 (MQ=255)                                                                     tgctgcAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGG‑GGGGTTTTTTGTGATCAATTTCAAAATAAAAAAAAAGATCCGATAAAAATAAAAAAGCGTTTCAATTGATGTGGgtttt                                                                       >  2:292525/1‑139 (MQ=255)                                                                       gagacaggAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGG‑TGGTTTTTTTGTGATCAATTTCAAAATAAAAACAATGATCCGATAAAAATAAAACAGCGTTTCAATTGATGTGGTTTTGAt                                                                     <  1:778160/133‑1 (MQ=255)                                                                       ctgcAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGG‑TGGTTTTTTTGTGATCAATTTCAAAATAAAAACAATGATCCGATAAAAATAAAACAGCGTTTCAAt                                                                                    <  2:71276/125‑1 (MQ=255)                                                                       ctgcAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGG‑TGGTTTTTTTGTGATCAATTTCAAAATAAAAACAATGATCCGATAAAAATAAAACAGCGTTTCAAt                                                                                    >  1:71276/1‑125 (MQ=255)                                                                       ctgcAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGG‑TGGTTTTTTTGTGATCAATTTCAAAATAAAAACAATGATCCGATAAAAATAAAACAGCGTTTCAATTGATGTGGTTTTGAt                                                                     <  2:725774/140‑1 (MQ=255)                                                                       ctgcAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGG‑TGGTTTTTTTGTGATCAATTTCAAAATAAAAACAATGATCCGATAAAAATAAAACAGCGTTTCAATTGATGTGGTTTTGAt                                                                     <  2:222213/140‑1 (MQ=255)                                                                       ctgcAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGG‑TGGTTTTTTTGTGATCAATTTCAAAATAAAAACAATGATCCGATAAAAATAAAACAGCGTTTCAATTGATGTGGTTTTGAt                                                                     <  1:1554857/140‑1 (MQ=255)                                                                          cAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGG‑TGGTTTTTTTGTGATCAATTTCAAAATAAAAACAATGATCCGATAAAAATAAAACAGCGTTTCAATTGATGTGGTTTTGATCAc                                                                  <  1:412459/140‑1 (MQ=255)                                                                             tGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGG‑TGGGTTTTTTTTGATCAATTTCAAAATAAAAACAATGATCCGATAAAAATAAAAAAGCGTTTCAATTGATGTGGTTTTGATCACttt                                                               >  1:1391692/1‑140 (MQ=255)                                                                               aGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGG‑TGGTTTTTTTGTGATCAATTTCAAAATAAAAACAATGATCCGATAAAAATAAAACAGCGTTTCAATTGATGTGGTTTTGATCACTTTTa                                                             <  2:289055/140‑1 (MQ=255)                                                                               aGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGG‑TGGTTTTTTTGTGATCAATTTCAAAATAAAAACAATGATCCGATAAAAATAAAACAGCGTTTCAATTGATGTGGTTTTGATCACTTTTa                                                             <  1:1495180/140‑1 (MQ=255)                                                                               aGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGG‑TGGTTTTTTTGTGATCAATTTCAAAATAAAAACAATGATCCGATAAAAATAAAACAGCGTTTCAATTGATGTGGTTTTGATCACTTTTa                                                             <  1:1683752/140‑1 (MQ=255)                                                                               aGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGG‑TGGTTTTTTTGTGATCAATTTCAAAATAAAAACAATGATCCGATAAAAATAAAACAGCGTTTCAATTGATGTGGTTTTGATCACTTTTa                                                             <  1:1355816/140‑1 (MQ=255)                                                                               aGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGG‑TGGGTTTTTTTTGATCAAATTTAAAATAAAAAAAAAGATCCGATAAAAAAAAAAAAACGTTTCAATTTATGTGGGTTTTATCACttttt                                                             >  2:1828069/1‑139 (MQ=255)                                                                                  cGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGG‑TGGGTTTTTTTTGATTAATTTTAAAATAAAAAAAATGATCCGATAAAAATAAAACAGCGTTTTAATTGATGTGGGTTTTATCACTttttttt                                                          >  2:249618/1‑139 (MQ=255)                                                                                  cGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGG‑TGGGTTTTTTGTGATCAATTTCAAAATAAAAAAAATGATCCGATAAAAATAAAACAGCGTTTTAATTGATGTGGTTTTGATCACTTTTAttt                                                          >  1:146071/1‑139 (MQ=255)                                                                                   gTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGG‑TGGTTTTTTTGTGATCAATTTCAAAATAAAAACAATGATCCGATAAAAATAAAACAGCGTTTCAATTGATGTGGTTTTGATCACTTTTATTGa                                                         <  2:1069254/140‑1 (MQ=255)                                                                                         gCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGG‑TGGTTTTTTTGTGATCAATTTCAAAATAAAAACAATGATCCGATAAAAATAAAACAGCGTTTCAATTGATGTGGTTTTGATCACTTTTATTGATTAaat                                                   <  2:944213/140‑1 (MQ=255)                                                                                         gCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGG‑TGGGTTTTTTTTGATCAATTTTAAAATAAAAACAATGATCCGGTAAAAAAAAAAAAACGTTTTAATTGATGTGGGTTTTATCACTTTTTTTTATTAaat                                                   >  2:623515/1‑140 (MQ=255)                                                                                         gCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGG‑TGGGTTTTTTTTGATCAATTTTAAAATAAAAAAAATGATCCGATAAAAATAAAAAAGCGGTTCAATTGATGTGGTTTTGTTTACTTTTTTTTATTaaaa                                                   >  2:725805/1‑139 (MQ=255)                                                                                           cGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGG‑TGGTTTTTTTGTGATCAATTTCAAAATAAAAACAATGATCCGATAAAAATAAAACAGCGTTTCAATTGATGTGGTTTTGATCACTTTTATTGATTAaatga                                                 >  1:966386/1‑140 (MQ=255)                                                                                           cGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGG‑TGGTTTTTTTGTGATCAATTTCAAAATAAAAACAATAATCCGATAAAAATAAAAAAGCGTTTCAAATTAAGTGGTTTTGATCACTTTTATTGATTAaatga                                                 >  2:706211/1‑140 (MQ=255)                                                                                           cGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGG‑TGGGTTTTTTTTGATCAATTTTTAAAAAAAAAAAAAGATCCCATAAAAAAAAAAAAACGTTTTAATTTTTGTGGGTTTTATTACTTTTTTTGATTAaaaga                                                 >  2:435869/1‑140 (MQ=255)                                                                                           cGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGG‑TGGGTTTTTTTTGATCAATTTTAAAATAAAAACAATGATCCGATAAAAATAAAAAAGCGTTTTAATTGATGTGGTTTTTATCACTTTTTTTTATTTaatga                                                 >  1:1481634/1‑140 (MQ=255)                                                                                           cGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGG‑TGGGTTTTTTGTGGTCAATTTCAAAATAAAAAAAAAGATCCGATAAAAATAAAAAAACGTTTTAATTTATGTGGGTTTTATCACTTTTTTTGATTAaatga                                                 >  2:20610/1‑140 (MQ=255)                                                                                             cATATCAGCTTAAAAAATGAACCATCGCCAACGGCGG‑TTGGTTTTTTGTTATTAATTTAAAAATAAAAAAAATGATCCGATAAAAATAAAACAGACATTCAATTGATGTGGTTTTTATCACTTTTATTTATTAaatgaaa                                               >  2:1739693/1‑139 (MQ=255)                                                                                             cATATCAGCTTAAAAAATGAACCATCGCCAACGGCGG‑TGGTTTTTTTTTGATCAATTTTAAAAAAAAAACAAAGATCCGATAAAAAAAAAAAAGCGTTTTAATTTATGTGGTTTTTATCACTTTTATTTATTAaaagaaa                                               >  2:198720/1‑139 (MQ=255)                                                                                              atatCAGCTTAAAAAATGAACCATCGCCAACGGCGG‑TGGTTTTTTTGTGATCAATTTTAAAATAAAAACAATGATCCGATAAAAATAAAACAGCGTTTCAATTGATGTGGTTTTGATCACTTTTATTTATTAaattaatt                                              >  1:1650164/1‑139 (MQ=255)                                                                                               tatCAGCTTAAAAAATGAACCATCGCCAACGGCGG‑TGGTTTTTTTGTGATCAATTTCAAAATAAAAACAATGATCCGATAAAAATAAAACAGCGTTTCAATTGATGTGGTTTTGATCACTTTTATTGATTAAATGAATGt                                             <  1:20610/140‑1 (MQ=255)                                                                                               tatCAGCTTAAAAAATGAACCATCGCCAACGGCGG‑TGGTTTTTTTGTGATCAATTTCAAAATAAAAACAATGATCCGATAAAAATAAAACAGCGTTTCAATTGATGTGGTTTTGATCACTTTTATTGATTAAATGAATGt                                             <  1:725805/140‑1 (MQ=255)                                                                                                atCAGCTTAAAAAATGAACCATCGCCAACGGCGG‑TGGTTTTTTTGTGATCAATTTCAAAATAAAAACAATGATCCGATAAAAATAAAACAGCGTTTCAATTGATGTGGTTTTGATCACTTTTATTGATTAAATGAATGTc                                            <  2:690561/140‑1 (MQ=255)                                                                                                   aGCTTAAAAAATGAACCATCGCCAACGGCGG‑TGGGTTTTTTTTTATTAATTTTAAAAAAAAAAAAAATATCCCATAAAAAAAAAACAACGGTTCAATTTATGTGGGTTTGATCACTTTTTTTTTTTAAAATAATGTCTAt                                         >  2:890804/1‑140 (MQ=255)                                                                                                     cTTAAAAAATGAACCATCGCCAACGGCGG‑TGGTTTTTTTGTGATCAATTTCAAAATAAAAACAATGATCCGATAAAAATAAAACAGCGTTTCAATTGATGTGGTTTTGATCACTTTTATTGATTAAATGAATGTCTATCt                                       <  1:143345/140‑1 (MQ=255)                                                                                                      ttAAAAAATGAACCATCGCCAACGGCGG‑TGGGTTTTTTGTGATCAATTTTAAAAAAAAAAAAATGATCCCATAAAAATAAAAAAGCGTTTTAATTGATGTGGGTTTGATCACTTTTTTTTATTAAATGAATGTCTATCtt                                      >  2:1387784/1‑140 (MQ=255)                                                                                                          aaaaTGAACCATCGCCAACGGCGG‑TGGTTTTTTTGTGATCAATTTCAAAATAAAAACAATGATCCGATAAAAATAAAACAGCGTTTCAATTGATGTGGTTTTGATCACTTTTATTGATTAAATGAATGTCTATCTTCGtt                                  <  2:1481634/140‑1 (MQ=255)                                                                                                          aaaaTGAACCATCGCCAACGGCGG‑TGGTTTTTTTGTGATCAATTTCAAAATAAAAACAATGATCCGATAAAAATAAAACAGCGTTTCAATTGATGTGGTTTTGATCACTTTTATTGATTAAATGAATGTCTATCTTCGtt                                  <  2:584275/140‑1 (MQ=255)                                                                                                          aaaaTGAACCATCGCCAACGGCGG‑TGGTTTTTTTGTGATCAATTTCAAAATAAAAACAATGATCCGATAAAAATAAAACAGCGTTTCAATTGATGTGGTTTTGATCACTTTTATTGATTAAATGAATGTCTATCTTCGtt                                  >  1:945127/1‑140 (MQ=255)                                                                                                          aaaaTGAACCATCGCCAACGGCGG‑TGGGTTTTTTTTGATCAATTTTAAAATAAAAAAAAAGATCCGATAAAAATAAAACAGCGTTTTAATTTATGTGGGTTTTATTACTTTTATTTATTAAATGAATGTCTATCTTCGtt                                  >  2:1725368/1‑140 (MQ=255)                                                                                                           aaaTGAACCATCGCCAACGGCGG‑TGGTTTTTTTGTGATCAATTTCAAAATAAAAACAATGATCCGATAAAAATAAAACAGCGTTTCAATTGATGTGGTTTTGATCTCTTTTATTGATTAAATGAATGTCTATCTTCGttt                                 <  1:622518/140‑1 (MQ=255)                                                                                                             aTGAACCATCGCCAACGGCGG‑TGGTTTTTTTGTGATCAATTTCAAAATAAAAACAATGATCCGATAAAAATAAAACAGCGTTTCAATTGATGTGGTTTTGATCACTTTTATTGATTAAATGAATGTCTATCTTCGTTTcc                               <  1:1327375/140‑1 (MQ=255)                                                                                                                  ccATCGCCAACGGCGG‑TGGTTTTTTTGTGATCAATTTCAAAATAAAAACAATGATCCGATAAAAATAAAACAGCGTTTCAATTGATGTGGTTTTGATCACTTTTATTGATTAAATGAATGTCTATCTTCGTTTCCATCaa                          <  1:1098370/140‑1 (MQ=255)                                                                                                                  ccATCGCCAACGGCGG‑TGGTTTTTTTGTGATCAATTTCAAAATAAAAACAATGATCCGATAAAAATAAAACAGCGTTTCAATTGATGTGGTTTTGATCACTTTTATTGATTAAATGAATGTCTATCTTCGTTTCCATCaa                          <  2:1206289/140‑1 (MQ=255)                                                                                                                  ccATCGCCAACGGCGG‑TGGTTTTTTTGTGATCAATTTCAAAATAAAAACAATGATCCGATAAAAATAAAACAGCGTTTCAATTGATGTGGTTTTGATCACTTTTATTGATTAAATGAATGTCTATCTTCGTTTCCATCaa                          <  1:1828069/140‑1 (MQ=255)                                                                                                                     tCGCCAACGGCGG‑TGGTTTTTTTGTGATCAATTTCAAAATAAAAACAATGATCCGATAAAAATAAAACAGCGTTTCAATTGATGTGGTTTTGATCACTTTTATTGATTAAATGAATGTCTATCTTCGTTTCCATCAacac                       <  1:962491/140‑1 (MQ=255)                                                                                                                      cGCCAACGGCGG‑TGGGTTTTTTTTGATCAATTTTAAAATAAAAAAAAAGATCCGATAAAAAAAAAAAAGCGTTTCAATTTATGTGGGTTTTATCACTTTTTTTTATTAAATGAATGTCTATCTTTGTTTTCATCAACACt                      >  2:268208/1‑140 (MQ=255)                                                                                                                      cGCCAACGGCGG‑TGGGTTTTTTTTGATCAATTTCAAAAAAAAAACAAAGATCCGATAAAAATAAAAAAGCGTTTTAATTGATGTGGGTTTTATCACTTTTTTTGATTAAATGAATGTCTATCTTCGTTTCCATCAACACt                      >  2:740771/1‑140 (MQ=255)                                                                                                                       gCCAACGGCGG‑TGGTTTTTTTGTGATCAATTTCAAAATAAAAACAATGATCCGATAAAAATAAAACAGCGTTTCAATTGATGTGGTTTTGATCACTTTTATTGATTAAATGAATGTCTATCTTCGTTTCCATCAACACTg                     <  1:1041349/140‑1 (MQ=255)                                                                                                                          aaCGGCGG‑TGGTTTTTTTGTGATCAATTTCAAAATAAAAACAATGATCCGATAAAAATAAAACAGCGTTTCAATTGATGTGGTTTTGATCACTTTTATTGATTAAATGAATGTCTATCTTCGTTTCCATCAACACTGATg                  <  1:198720/140‑1 (MQ=255)                                                                                                                          aaCGGCGG‑TGGTTTTTTTGTGATCAATTTCAAAATAAAAACAATGATCCGATAAAAATAAAACAGCGTTTCAATTGATGTGGTTTTGATCACTTTTATTGATTAAATGAATGTCTATCTTCGTTTCCATCAACACTGATg                  <  2:945127/140‑1 (MQ=255)                                                                                                                          aaCGGCGG‑TGGTTTTTTTGTGATCAATTTCAAAATAAAAACAATGATCCGATAAAAATAAAACAGCGTTTCAATTGATGTGGTTTTGATCACTTTTATTGATTAAATGAATGTCTATCTTCGTTTCCATCAACACTGATg                  <  2:966386/140‑1 (MQ=255)                                                                                                                            cggcgg‑TGGTTTTTTTGTGATCAATTTCAAAATAAAAACAATGATCCGATAAAAATAAAACAGCGTTTCAATTGATGTGGTTTTGATCACTTTTATTGATTAAATGAATGTCTATCTTCGTTTCCATCAACACTGATGCt                <  2:1109033/140‑1 (MQ=255)                                                                                                                             ggcgg‑TGGGTTTTTTGTGATCAATTTCAAAATAAAAACAATGATCCGATAAAAATAAAACAGCGTTTCAATTGATGTGGTTTTGATCACTTTTATTGATTAAATGAATGTCTATCTTCGTTTCCATCAACACTGATGCTc               >  2:487872/1‑140 (MQ=255)                                                                                                                               cgg‑cggCTTTTTTGTGGTGAATTTCAAAATAAAAACAAAAATCCGGTAAAAATAAAAAAACGTTTTAATTTTTGTGGTTTTTATCACTTTTTTTGATTAAATGAATGTCTTTCTTCGCTTCCATCAACACTGATGCTCCa             >  2:1777515/1‑140 (MQ=255)                                                                                                                               cgg‑TGGTTTTTTTTTGATCAATTTTAAAATAAAAACAAAGATCCCATAAAAATAAAAAAACGTTTTAATTGATGTGGGTTTTATCACTTTTATTGATTAAATGAATGTCTATCTTCGTTTTCATCAACACTGATGCTccc             >  2:1347964/1‑139 (MQ=255)                                                                                                                               cgg‑TGGTTTTTTTGTGATCAATTTCAAAATAAAAACAATGATCCGATAAAAATAAAACAGCGTTTCAATTGATGTGGTTTTGATCACTTTTATTGATTAAATGAATGTCTATCTTCGTTTCCATCAACACTGATGCTCCa             <  1:731338/140‑1 (MQ=255)                                                                                                                                 g‑tgggTTTTTTTTGATCAATTTTAAAATAAAAAAAATGATCCGATAAAAATAAAAAAGCGTTTCAATTGATGTGGGTTTGATCACTTTTATTGATTAAATGAATGTCTATCTTCGTTTCCATCAACACTGATGCTCCAtt           >  2:643393/1‑140 (MQ=255)                                                                                                                                     gtTTTTTTGTGATCAATTTCAAAATAAAAACAATGATCCGATAAAAATAAAACAGCGTTTCAATTGATGTGGTTTTGATCACTTTTATTGATTAAATGAATGTCTATCTTCGTTTCCATCAACACTGATGCTCCATTGAg        <  1:750220/140‑1 (MQ=255)                                                                                                                                      tttttttGTGATCAATTTCAAAATAAAAACAATGATCCGATAAAAATAAAACAGCGTTTCAATTGATGTGGTTTTGATCACTTTTATTGATTAAATGAATGTCTATCTTCGTTTCCATCAACACTGATGCTCCATTGAgg       <  2:654474/140‑1 (MQ=255)                                                                                                                                         ttttGTGATCAATTTCAAAATAAAAACAATGATCCGATAAAAATAAAACAGCGTTTCAATTGATGTGGTTTTGATCACTTTTATTGATTAAATGAATGTCTATCTTCGTTTCCATCAACACTGATGCTCCATTGAGGAAt    <  1:1725368/140‑1 (MQ=255)                                                                                                                                           ttGTGATCAATTTCAAAATAAAAACAATGATCCGATAAAAATAAAACAGCGTTTCAATTGATGTGGTTTTGATCACTTTTATTGATTAAATGAATGTCTATCTTCGTTTCCATCAACACTGATGCTCCATTGAGGAATTa  <  1:326398/140‑1 (MQ=255)                                                                                                                                             |                                                                                                                                          TCTGGAACGTGGTGAACTGGGACGAAGCAGCGGCACGTTTTGCGGCGAAAAAATAATCATTTGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGG‑TGGTTTTTTTGTGATCAATTTCAAAATAAAAACAATGATCCGATAAAAATAAAACAGCGTTTCAATTGATGTGGTTTTGATCACTTTTATTGATTAAATGAATGTCTATCTTCGTTTCCATCAACACTGATGCTCCATTGAGGAATTA  >  NC_000913/4101375‑4101651

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑