Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,267,777	A→G	D153G (GAC→GGC)	ychA →	transglutaminase‑like/TPR repeat‑containing protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,267,777	0	A	G	100.0%	361.5 / NA	91	D153G (GAC→GGC)	ychA	transglutaminase‑like/TPR repeat‑containing protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (42/49);  total (42/49)

GGTCGCGAATCGTCTCGATTTGCCGCTGCTGCCGGTGATTTTCCCTACGCAGCTGATATTGCGCATTGAATGTCCGGATGGCGAAATTTGGCTGATTAATCCTTTTAACGGTGAATCGTTAAGCGAACATATGCTGGACGTATGGTTAAAGGGAAATATCAGCCCGTCGGCGGAACTGTTTTATGAAGACCTTGATGAAGCTGATAACATTGAGGTAATCCGCAAATTGCTGGATACACTCAAAGCCTCGTTGATGGAAGAAAATCAGATGG  >  NC_000913/1267640‑1267911                                                                                                                                          |                                                                                                                                       ggTCGCGAATCGTCTCGATTTGCCGCTGCTGCCGGTGATTTTCCCTACGCAGCTGATATTGCGCATTGAATGTCCGGATGGCGAAATTTGGCTGATTAATCCTTTTAACGGTGAATCGTTAAGCGAACATATGCTGGGCg                                                                                                                                      >  2:988609/1‑140 (MQ=255) ggTCGCGAATCGTCTCGATTTGCCGCTGCTGCCGGTGATTTTCCCTACGCAGCTGATATTGCGCATTGAATGTCCGGATGGCGAAATTTGGCTGATTAATCCTTTTAACGGTGAATCGTTAAGCGAACATATGCTGGGCg                                                                                                                                      >  2:1460605/1‑140 (MQ=255) ggTCGCGAATCGTCTCGATTTGCCGCTGCTGCCGGTGATTTTCCCTACGCAGCTGATATTGCGCATTGAATGTCCGGATGGCGAAATTTGGCTGATTAATCCTTTTAACGGTGAATCGTTAAGCGAACATATGCTGGGCg                                                                                                                                      >  1:47690/1‑140 (MQ=255) ggTCGCGAATCGTCTCGATTTGCCGCTGCTGCCGGTGATTTTCCCTACGCAGCTGATATTGCGCATTGAATGTCCGGATGGCGAAATTTGGCTGATTAATCCTTTTAACGGTGAATCGTTAAGCGAACATATGCTGGGCg                                                                                                                                      >  2:738022/1‑140 (MQ=255)   tCGCGAATCGTCTCGATTTGCCGCTGCTGCCGGTGATTTTCCCTACGCAGCTGATATTGCGCATTGAATGTCCGGATGGCGAAATTTGGCTGATTAATCCTTTTAACGGTGAATCGTTAAGCGAACATATGCTGGGCGTa                                                                                                                                    <  2:935178/140‑1 (MQ=255)    cgcgAATCGTCTCGATTTGCCGCTGCTGCCGGTGATTTTCCCTACGCAGCTGATATTGCGCATTGAATGTCCGGATGGCGAAATTTGGCTGATTAATCCTTTTAACGGTGAATCGTTAAGCGAACATATGCTGGGCGTAt                                                                                                                                   <  2:777819/140‑1 (MQ=255)     gcgAATCGTCTCGATTTGCCGCTGCTGCCGGTGATTTTCCCTACGCAGCTGATATTGCGCATTGAATGTCCGGATGGCGAAATTTGGCTGATTAATCCTTTTAACGGTGAATCGTTAAGCGAACATATGCTGGGCGTATg                                                                                                                                  >  2:323934/1‑140 (MQ=255)      cgAATCGTCTCGATTTGCCGCTGCTGCCGGTGATTTTCCCTACGCAGCTGATATTGCGCATTGAATGTCCGGATGGCGAAATTTGGCTGATTAATCCTTTTAACGGTGAATCGTTAAGCGAACATATGCTGGGCGTATgg                                                                                                                                 >  2:216437/1‑140 (MQ=255)          tCGTCTCGATTTGCCGCTGCTGCCGGTGATTTTCCCTACGCAGCTGATATTGCGCATTGAATGTCCGGATGGCGAAATTTGGCTGATTAATCCTTTTAACGGTGAATCGTTAAGCGAACATATGCTGGGCGTATGGTTaa                                                                                                                             <  2:434472/140‑1 (MQ=255)          tCGTCTCGATTTGCCGCTGCTGCCGGTGATTTTCCCTACGCAGCTGATATTGCGCATTGAATGTCCGGATGGCGAAATTTGGCTGATTAATCCTTTTAACGGTGAATCGTTAAGCGAACATATGCTGGGCGTATGGTTaa                                                                                                                             <  2:745311/140‑1 (MQ=255)           cGTCTCGATTTGCCGCTGCTGCCGGTGATTTTCCCTACGCAGCTGATATTGCGCATTGAATGTCCGGATGGCGAAATTTGGCTGATTAATCCTTTTAACGGTGAATCGTTAAGCGAACATATGCTGGGCGTATGGTTaaa                                                                                                                            >  2:486037/1‑140 (MQ=255)             tctcGATTTGCCGCTGCTGCCGGTGATTTTCCCTACGCAGCTGATATTGCGCATTGAATGTCCGGATGGCGAAATTTGGCTGATTAATCCTTTTAACGGTGAATCGTTAAGCGAACATATGCTGGGTGTATGGTTAAAgg                                                                                                                          >  1:348951/1‑140 (MQ=255)             tctcGATTTGCCGCTGCTGCCGGTGATTTTCCCTACGCAGCTGATATTGCGCATTGAATGTCCGGATGGCGAAATTTGGCTGATTAATCCTTTTAACGGTGAATCGTTAAGCGAACATATGCTGGGCGTATGGTTAAAgg                                                                                                                          >  1:439769/1‑140 (MQ=255)             tctcGATTTGCCGCTGCTGCCGGTGATTTTCCCTACGCAGCTGATATTGCGCATTGAATGTCCGGATGGCGAAATTTGGCTGATTAATCCTTTTAACGGTGAATCGTTAAGCGAACATATGCTGGGCGTATGGTTAAAgg                                                                                                                          <  1:1187811/140‑1 (MQ=255)                       ccGCTGCTGCCGGTGATTTTCCCTACGCAGCTGATATTGCGCATTGAATGTCCGGATGGCGAAATTTGGCTGATTAATCCTTTTAACGGTGAATCGTTAAGCGAACATATGCTGGGCGTATGGTTAAAGGGAAATATCAg                                                                                                                >  1:247496/1‑140 (MQ=255)                        cGCTGCTGCCGGTGATTTTCCCTACGCAGCTGATATTGCGCATTGAATGTCCGGATGGCGAAATTTGGCTGATTAATCCTTTTAACGGTGAATCGTTAAGCGAACATATGCTGGGCGTATGGTTAAAGGGAAATATCAGc                                                                                                               >  2:208256/1‑140 (MQ=255)                           tgctgcCGGTGATTTTCCCTACGCAGCTGATATTGCGCATTGAATGTCCGGATGGCGAAATTTGGCTGATTAATCCTTTTAACGGTGAATCGTTAAGCGAACATATGCTGGGCGTATGGTTAAAGGGAAATATCAGccc                                                                                                             >  1:1363006/1‑139 (MQ=255)                           tgctgcCGGTGATTTTCCCTACGCAGCTGATATTGCGCATTGAATGTCCGGATGGCGAAATTTGGCTGATTAATCCTTTTAACGGTGAATCGTTAAGCGAACATATGCTGGGCGTATGGTTAAAGGGAAATATCAGccc                                                                                                             <  2:1363006/139‑1 (MQ=255)                            gctgcCGGTGATTTTCCCTACGCAGCTGATATTGCGCATTGAATGTCCGGATGGCGAAATTTGGCTGATTAATCCTTTTAACGGTGAATCGTTAAGCGAACATATGCTGGGCGTATGGTTAAAGGGAAATATCAGCCCGt                                                                                                           >  2:1611478/1‑140 (MQ=255)                               gcCGGTGATTTTCCCTACGCAGCTGATATTGCGCATTGAATGTCCGGATGGCGAAATTTGGCTGATTAATCCTTTTAACGGTGAATCGTTAAGCGAACATATGCTGGGCGTATGGTTAAAGGGAAATATCAGCCCGTcgg                                                                                                        <  1:319828/140‑1 (MQ=255)                                    tGATTTTCCCTACGCAGCTGATATTGCGCATTGAATGTCCGGATGGCGAAATTTGGCTGATTAATCCTTTTAACGGTGAATCGTTAAGCGAACATATGCTGGGCGTATGGTTAAAGGGAAATATCAGCCCGTCGGCGGaa                                                                                                   <  1:738022/140‑1 (MQ=255)                                    tGATTTTCCCTACGCAGCTGATATTGCGCATTGAATGTCCGGATGGCGAAATTTGGCTGATTAATCCTTTTAACGGTGAATCGTTAAGCGAACATATGCTGGGCGTATGGTTAAAGGGAAATATCAGCCCGTCGGCGGaa                                                                                                   <  1:675095/140‑1 (MQ=255)                                    tGATTTTCCCTACGCAGCTGATATTGCGCATTGAATGTCCGGATGGCGAAATTTGGCTGATTAATCCTTTTAACGGTGAATCGTTAAGCGAACATATGCTGGGCGTATGGTTAAAGGGAAATATCAGCCCGTCGGCGGaa                                                                                                   <  1:125708/140‑1 (MQ=255)                                    tGATTTTCCCTACGCAGCTGATATTGCGCATTGAATGTCCGGATGGCGAAATTTGGCTGATTAATCCTTTTAACGGTGAATCGTTAAGCGAACATATGCTGGGCGTATGGTTAAAGGGAAATATCAGCCCGTCGGCGGaa                                                                                                   <  2:47690/140‑1 (MQ=255)                                     gATTTTCCCTACGCAGCTGATATTGCGCATTGAATGTCCGGATGGCGAAATTTGGCTGATTAATCCTTTTAACGGTGAATCGTTAAGCGAACATATGCTGGGCGTATGGTTAAAGGGAAATATCAGCCCGTCGGCGGAAc                                                                                                  <  2:1379402/140‑1 (MQ=255)                                       ttttCCCTACGCAGCTGATATTGCGCATTGAATGTCCGGATGGCGAAATTTGGCTGATTAATCCTTTTAACGGTGAATCGTTAAGCGAACATATGCTGGGCGTATGGTTAAAGGGAAATATCAGCCCGTCGGCGGAACTg                                                                                                <  2:247496/140‑1 (MQ=255)                                        tttCCCTACGCAGCTGATATTGCGCATTGAATGTCCGGATGGCGAAATTTGGCTGATTAATCCTTTTAACGGTGAATCGTTAAGCGAACATATGCTGGGCGTATGGTTAAAGGGAAATATCAGCCCGTCGGCGGAACTGt                                                                                               <  2:45618/140‑1 (MQ=255)                                         ttCCCTACGCAGCTGATATTGCGCATTGAATGTCCGGATGGCGAAATTTGGCTGATTAATCCTTTTAACGGTGAATCGTTAAGCGAACATATGCTGGGCGTATGGTTAAAGGGAAATATCAGCCCGTCGGCGGAACTGtt                                                                                              <  1:1273565/140‑1 (MQ=255)                                          tCCCTACGCAGCTGATATTGCGCATTGAATGTCCGGATGGCGAAATTTGGCTGATTAATCCTTTTAACGGTGAATCGTTAAGCGAACATATGCTGGGCGTATGGTTAAAGGGAAATATCAGCCCGTCGGCGGAACTGttt                                                                                             >  2:1230024/1‑140 (MQ=255)                                              tACGCAGCTGATATTGCGCATTGAATGTCCGGATGGCGAAATTTGGCTGATTAATCCTTTTAACGGTGAATCGTTAAGCGAACATATGCTGGGCGTATGGTTAAAGGGAAATATCAGCCCGTCGGCGGAACTGtt                                                                                              >  1:2610/1‑135 (MQ=255)                                              tACGCAGCTGATATTGCGCATTGAATGTCCGGATGGCGAAATTTGGCTGATTAATCCTTTTAACGGTGAATCGTTAAGCGAACATATGCTGGGCGTATGGTTAAAGGGAAATATCAGCCCGTCGGCGGAACTGtt                                                                                              <  2:2610/135‑1 (MQ=255)                                              tACGCAGCTGATATTGCGCATTGAATGTCCGGATGGCGAAATTTGGCTGATTAATCCTTTTAACGGTGAATCGTTAAGCGAACATATGCTGGGCGTATGGTTAAAGGGAAATATCAGCCCGTCGGCGGAACTGTTTTATg                                                                                         <  1:753318/140‑1 (MQ=255)                                              tACGCAGCTGATATTGCGCATTGAATGTCCGGATGGCGAAATTTGGCTGATTAATCCTTTTAACGGTGAATCGTTAAGCGAACATATGCTGGGCGTATGGTTAAAGGGAAATATCAGCCCGTCGGCGGAACTGTTTTATg                                                                                         >  2:860998/1‑140 (MQ=255)                                               aCGCAGCTGATATTGCGCATTGAATGTCCGGATGGCGAAATTTGGCTGATTAATCCTTTTAACGGTGAATCGTTAAGCGAACATATGCTGGGCGTATGGTTAAAGGGAAATATCAGCCCGTCGGCGGAACTGTTTTATGa                                                                                        <  1:1460605/140‑1 (MQ=255)                                               aCGCAGCTGATATTGCGCATTGAATGTCCGGATGGCGAAATTTGGCTGATTAATCCTTTTAACGGTGAATCGTTAAGCGAACATATGCTGGGCGTATGGTTAAAGGGAAATATCAGCCCGTCGGCGGAACTGTTTTATGa                                                                                        <  1:1611478/140‑1 (MQ=255)                                                   aGCTGATATTGCGCATTGAATGTCCGGATGGCGAAATTTGGCTGATTAATCCTTTTAACGGTGAATCGTTAAGCGAACATATGCTGGGCGTATGGTTAAAGGGAAATATCAGCCCGTCGGCGGAACTGTTTTATGAAGAc                                                                                    <  1:173469/140‑1 (MQ=255)                                                   aGCTGATATTGCGCATTGAATGTCCGGATGGCGAAATTTGGCTGATTAATCCTTTTAACGGTGAATCGTTAAGCGAACATATGCTGGGCGTATGGTTAAAGGGAAATATCAGCCCGTCGGCGGAACTGTTTTATGAAGAc                                                                                    <  1:1821027/140‑1 (MQ=255)                                                     cTGATATTGCGCATTGAATGTCCGGATGGCGAAATTTGGCTGATTAATCCTTTTAACGGTGAATCGTTAAGCGAACATATGCTGGGCGTATGGTTAAAGGGAAATATCAGCCCGTCGGCGGAACTGTTTTATg                                                                                         >  2:263590/1‑133 (MQ=255)                                                     cTGATATTGCGCATTGAATGTCCGGATGGCGAAATTTGGCTGATTAATCCTTTTAACGGTGAATCGTTAAGCGAACATATGCTGGGCGTATGGTTAAAGGGAAATATCAGCCCGTCGGCGGAACTGTTTTATg                                                                                         <  1:263590/133‑1 (MQ=255)                                                     cTGATATTGCGCATTGAATGTCCGGATGGCGAAATTTGGCTGATTAATCCTTTTAACGGTGAATCGTTAAGCGAACATATGCTGGGCGTATGGTTAAAGGGAAATATCAGCCCGTCGGCGGAACTGTTTTATGAAGACCt                                                                                  >  1:511834/1‑140 (MQ=255)                                                        atatTGCGCATTGAATGTCCGGATGGCGAAATTTGGCTGATTAATCCTTTTAACGGTGAATCGTTAAGCGAACATATGCTGGGCGTATGGTTAAAGGGAAATATCAGCCCGTCGGCGGAACTGTTTTATGAAGACCTtga                                                                               <  1:1116074/140‑1 (MQ=255)                                                        atatTGCGCATTGAATGTCCGGATGGCGAAATTTGGCTGATTAATCCTTTTAACGGTGAATCGTTAAGCGAACATATGCTGGGCGTATGGTTAAAGGGAAATATCAGCCCGTCGGCGGAACTGTTTTATGAAGACCTtga                                                                               <  1:1065955/140‑1 (MQ=255)                                                          atTGCGCATTGAATGTCCGGATGGCGAAATTTGGCTGATTAATCCTTTTAACGGTGAATCGTTAAGCGAACATATGCTGGGTGTATGGTTAAAGGGAAATATCAGCCCGTCGGCGGAACTGTTTTATGAAGACCTtgatg                                                                             <  2:348951/140‑1 (MQ=255)                                                          atTGCGCATTGAATGTCCGGATGGCGAAATTTGGCTGATTAATCCTTTTAACGGTGAATCGTTAAGCGAACATATGCTGGGCGTATGGTTAAAGGGAAATATCAGCCCGTCGGCGGAACTGTTTTATGAAGACCTtgatg                                                                             >  1:1149719/1‑140 (MQ=255)                                                          atTGCGCATTGAATGTCCGGATGGCGAAATTTGGCTGATTAATCCTTTTAACGGTGAATCGTTAAGCGAACATATGCTGGGCGTATGGTTAAAGGGAAATATCAGCCCGTCGGCGGAACTGTTTTATGAAGACCTtgatg                                                                             <  2:439769/140‑1 (MQ=255)                                                          atTGCGCATTGAATGTCCGGATGGCGAAATTTGGCTGATTAATCCTTTTAACGGTGAATCGTTAAGCGAACATATGCTGGGCGTATGGTTAAAGGGAAATATCAGCCCGTCGGCGGAACTGTTTTATGAAGACCTtgatg                                                                             <  1:1761622/140‑1 (MQ=255)                                                          atTGCGCATTGAATGTCCGGATGGCGAAATTTGGCTGATTAATCCTTTTAACGGTGAATCGTTAAGCGAACATATGCTGGGCGTATGGTTAAAGGGAAATATCAGCCCGTCGGCGGAACTGTTTTATGAAGACCTtgatg                                                                             <  1:208256/140‑1 (MQ=255)                                                           ttGCGCATTGAATGTCCGGATGGCGAAATTTGGCTGATTAATCCTTTTAACGGTGAATCGTTAAGCGAACATATGCTGGGCGTATGGTTAAAGGGAAATATCAGCCCGTCGGCGGAACTGTTTTATGAAGACCTtgatga                                                                            <  2:274274/140‑1 (MQ=255)                                                            tGCGCATTGAATGTCCGGATGGCGAAATTTGGCTGATTAATCCTTTTAACGGTGAATCGTTAAGCGAACATATGCTGGGCGTATGGTTAAAGGGAAATATCAGCCCGTCGGCGGAACTGTTTTATGAAGACCTTGATGaa                                                                           <  2:535707/140‑1 (MQ=255)                                                             gcgcATTGAATGTCCGGATGGCGAAATTTGGCTGATTAATCCTTTTAACGGTGAATCGTTAAGCGAACATATGCTGGGCGTATGGTTAAAGGGAAATATCAGCCCGTCGGCGGAACTGTTTTATGAAGACCTTGATGAAg                                                                          <  1:323934/140‑1 (MQ=255)                                                               gcATTGAATGTCCGGATGGCGAAATTTGGCTGATTAATCCTTTTAACGGTGAATCGTTAAGCGAACATATGCTGGGCGTATGGTTAAAGGGAAATATCAGCCCGTCGGCGGAACTGTTTTATGAAGACCTTGATGAAGCt                                                                        >  1:1276080/1‑140 (MQ=255)                                                               gcATTGAATGTCCGGATGGCGAAATTTGGCTGATTAATCCTTTTAACGGTGAATCGTTAAGCGAACATATGCTGGGCGTATGGTTAAAGGGAAATATCAGCCCGTCGGCGGAACTGTTTTATGAAGACCTTGATGAAGCt                                                                        >  2:338126/1‑140 (MQ=255)                                                               gcATTGAATGTCCGGATGGCGAAATTTGGCTGATTAATCCTTTTAACGGAGAATCGTTAAGCGAACATATGCTGGGCGTATGGTTAAAGGGAAATATCAGCCCGTCGGCGGAACTGTTTTATGAAGACCTTGATGAAGCt                                                                        >  1:1160511/1‑140 (MQ=255)                                                                 aTTGAATGTCCGGATGGCGAAATTTGGCTGATTAATCCTTTTAACGGTGAATCGTTAAGCGAACATATGCTGGGCGTATGGTTAAAGGGAAATATCAGCCCGTCGGCGGAACTGTTTTATGAAGACCTTGATGAAGCTGa                                                                      <  1:1420787/140‑1 (MQ=255)                                                                  ttGAATGTCCGGATGGCGAAATTTGGCTGATTAATCCTTTTAACGGTGAATCGTTAAGCGAACATATGCTGGGCGTATGGTTAAAGGGAAATATCAGCCCGTCGGCGGAACTGTTTTATGAAGACCTTGATGAAGCTGAt                                                                     <  2:1276080/140‑1 (MQ=255)                                                                  ttGAATGTCCGGATGGCGAAATTTGGCTGATTAATCCTTTTAACGGTGAATCGTTAAGCGAACATATGCTGGGCGTATGGTTAAAGGGAAATATCAGCCCGTCGGCGGAACTGTTTTATGAAGACCTTGATGAAGCTGAt                                                                     <  1:860998/140‑1 (MQ=255)                                                                  ttGAATGTCCGGATGGCGAAATTTGGCTGATTAATCCTTTTAACGGTGAATCGTTAAGCGAACATATGCTGGGCGTATGGTTAAAGGGAAATATCAGCCCGTCGGCGGAACTGTTTTATGAAGACCTTGATGAAGCTGAt                                                                     <  2:455422/140‑1 (MQ=255)                                                                  ttGAATGTCCGGATGGCGAAATTTGGCTGATTAATCCTTTTAACGGTGAATCGTTAAGCGAACATATGCTGGGCGTATGGTTAAAGGGAAATATCAGCCCGTCGGCGGAACTGTTTTATGAAGACCTTGATGAAGCTGAt                                                                     <  2:764906/140‑1 (MQ=255)                                                                   tGAATGTCCGGATGGCGAAATTTGGCTGATTAATCCTTTTAACGGTGAATCGTTAAGCGAACATATGCTGGGCGTATGGTTAAAGGGAAATATCAGCCCGTCGGCGGAACTGTTTTATGAAGACCTTGATGAAGCTGATa                                                                    >  1:519413/1‑140 (MQ=255)                                                                             gATGGCGAAATTTGGCTGATTAATCCTTTTAACGGTGAATCGTTAAGCGAACATATGCTGGGCGTATGGTTAAAGGGAAATATCAGCCCGTCGGCGGAACTGTTTTATGAAGACCTTGATGAAGCTGATAACATTGAGGt                                                          >  1:533772/1‑140 (MQ=255)                                                                             gATGGCGAAATTTGGCTGATTAATCCTTTTAACGGTGAATCGTTAAGCGAACATATGCTGGGCGCATGGTTAAAGGGAAATATCAGCCCGTCGGCGGAACTGTTTTATGAAGACCTTGATGAAGCTGATAACATTGAGGt                                                          >  2:1369874/1‑140 (MQ=255)                                                                               tGGCGAAATTTGGCTGATTAATCCTTTTAACGGTGAATCGTTAAGCGAACATATGCTGGGCGTATGGTTAAAGGGAAATATCAGCCCGTCGGCGGAACTGTTTTATGAAGACCTTGATGAAGCTGATAACATTGAGGTaa                                                        >  2:1699735/1‑140 (MQ=255)                                                                                  cGAAATTTGGCTGATTAATCCTTTTAACGGTGAATCGTTAAGCGAACATATGCTGGGCGTATGGTTAAAGGGAAATATCAGCCCGTCGGCGGAACTGTTTTATGAAGACCTTGATGAAGCTGATAACATTGAGGTAATcc                                                     >  1:1627578/1‑140 (MQ=255)                                                                                  cGAAATTTGGCTGATTAATCCTTTTAACGGTGAATCGTTAAGCGAACATATGCTGGGCGTATGGTTAAAGGGAAATATCAGCCCGTCGGCGGAACTGTTTTATGAAGACCTTGATGAAGCTGATAACATTGAGGTAATcc                                                     >  1:732476/1‑140 (MQ=255)                                                                                     aaTTTGGCTGATTAATCCTTTTAACGGTGAATCGTTAAGCGAACATATGCTGGGCGTATGGTTAAAGGGAAATATCAGCCCGTCGGCGGAACTGTTTTATGAAGACCTTGATGAAGCTGATAACATTGAGGTAATCCGCa                                                  <  2:732476/140‑1 (MQ=255)                                                                                       tttGGCTGATTAATCCTTTTAACGGTGAATCGTTAAGCGAACATATGCTGGGCGTATGGTTAAAGGGAAATATCAGCCCGTCGGCGGAACTGTTTTATGAAGACCTTGATGAAGCTGATAACATTGAGGTAATCCGCaaa                                                <  1:216437/140‑1 (MQ=255)                                                                                          ggCTGATTAATCCTTTTAACGGTGAATCGTTAAGCGAACATATGCTGGGCGTATGGTTAAAGGGAAATATCAGCCCGTCGGCGGAACTGTTTTATGAAGACCTTGATGAAGCTGATAACATTGAGGTAATCCGCAAATTg                                             >  1:733506/1‑140 (MQ=255)                                                                                           gCTGATTAATCCTTTTAACGGTGAATCGTTAAGCGAACATATGCTGGGCGTATGGTTAAAGGGAAATATCAGCCCGTCGGCGGAACTGTTTTATGAAGACCTTGATGAAGCTGATAACATTGAGGTAATCCGCAAATTGc                                            <  2:496675/140‑1 (MQ=255)                                                                                           gCTGATTAATCCTTTTAACGGTGAATCGTTAAGCGAACATATGCTGGGCGTATGGTTAAAGGGAAATATCAGCCCGTCGGCGGAACTGTTTTATGAAGACCTTGATGAAGCTGATAACATTGAGGTAATCCGCAAATTGc                                            <  1:486037/140‑1 (MQ=255)                                                                                                 tAATCCTTTTAACGGTGAATCGTTAAGCGAACATATGCTGGGCGTATGGTTAAAGGGAAATATCAGCCCGTCGGCGGAACTGTTTTATGAAGACCTTGATGAAGCTGATAACATTGAGGTAATCCGCAAATTGCTGGATa                                      >  1:717598/1‑140 (MQ=255)                                                                                                 tAATCCTTTTAACGGTGAATCGTTAAGCGAACATATGCTGGGCGTATGGTTAAAGGGAAATATCAGCCCGTCGGCGGAACTGTTTTATGAAGACCTTGATGAAGCTGATAACATTGAGGTAATCCGCAAATTGCTGGATa                                      <  2:217429/140‑1 (MQ=255)                                                                                                 tAATCCTTTTAACGGTGAATCGTTAAGCGAACATATGCTGGGCGTATGGTTAAAGGGAAATATCAGCCCGTCGGCGGAACTGTTTTATGAAGACCTTGATGAAGCTGATAACATTGAGGTAATCCGCAAATTGCTGGATa                                      <  2:1627578/140‑1 (MQ=255)                                                                                                  aaTCCTTTTAACGGTGAATCGTTAAGCGAACATATGCTGGGCGTATGGTTAAAGGGAAATATCAGCCCGTCGGCGGAACTGTTTTATGAAGACCTTGATGAAGCTGATAACATTGAGGTAATCCGCAAATTGCTGGATac                                     <  1:1699735/140‑1 (MQ=255)                                                                                                    tCCTTTTAACGGTGAATCGTTAAGCGAACATATGCTGGGCGTATGGTTAAAGGGAAATATCAGCCCGTCGGCGGAACTGTTTTATGAAGACCTTGATGAAGCTGATAACATTGAGGTAATCCGCAAATTGc                                            >  2:833114/1‑131 (MQ=255)                                                                                                    tCCTTTTAACGGTGAATCGTTAAGCGAACATATGCTGGGCGTATGGTTAAAGGGAAATATCAGCCCGTCGGCGGAACTGTTTTATGAAGACCTTGATGAAGCTGATAACATTGAGGTAATCCGCAAATTGc                                            <  1:833114/131‑1 (MQ=255)                                                                                                        tttAACGGTGAATCGTTAAGCGAACATATGCTGGGCGTATGGTTAAAGGGAAATATCAGCCCGTCGGCGGAACTGTTTTATGAAGACCTTGATGAAGCTGATAACATTGAGGTAATCCGCAAATTGCTGGATACACTCaa                               >  2:715164/1‑140 (MQ=255)                                                                                                         ttAACGGTGAATCGTTAAGCGAACATATGCTGGGCGTATGGTTAAAGGGAAATATCAGCCCGTCGGCGGAACTGTTTTATGAAGACCTTGATGAAGCTGATAACATTGAGGTAATCCGCAAATTGCTGGATACACTCaaa                              >  1:139825/1‑140 (MQ=255)                                                                                                         ttAACGGTGAATCGTTAAGCGAACATATGCTGGGCGTATGGTTAAAGGGAAATATCAGCCCGTCGGCGGAACTGTTTTATGAAGACCTTGATGAAGCTGATAACATTGAGGTAATCCGCAAATTGCTGGATACACTCaaa                              >  1:987200/1‑140 (MQ=255)                                                                                                         ttAACGGTGAATCGTTAAGCGAACATATGCTGGGCGTATGGTTAAAGGGAAATATCAGCCCGTCGGCGGAACTGTTTTATGAAGACCTTGATGAAGCTGATAACATTGAGGTAATCCGCAAATTGCTGGATACACTCaaa                              <  2:1149719/140‑1 (MQ=255)                                                                                                         ttAACGGTGAATCGTTAAGCGAACATATGCTGGGCGTATGGTTAAAGGGAAATATCAGCCCGTCGGCGGAACTGTTTTATGAAGACCTTGATGAAGCTGATAACATTGAGGTAATCCGCAAATTGCTGGATACACTCaaa                              >  2:1370675/1‑140 (MQ=255)                                                                                                          tAACGGTGAATCGTTAAGCGAACATATGCTGGGCGTATGGTTAAAGGGAAATATCAGCCCGTCGGCGGAACTGTTTTATGAAGACCTTGATGAAGCTGATAACATTGAGGTAATCCGCAAATTGCTGGATACACTCAAAg                             <  2:988379/140‑1 (MQ=255)                                                                                                               gTGAATCGTTAAGCGAACATATGCTGGGCGTATGGTTAAAGGGAAATATCAGCCCGTCGGCGGAACTGTTTTATGAAGACCTTGATGAAGCTGATAACATTGAGGTAATCCGCAAATTGCTGGATACACTCAAAGCCTCg                        <  2:733506/140‑1 (MQ=255)                                                                                                                 gAATCGTTAAGCGAACATATGCTGGGCGTATGGTTAAAGGGAAATATCAGCCCGTCGGCGGAACTGTTTTATGAAGACCTTGATGAAGCTGATAACATTGAGGTAATCCGCAAATTGCTGGATACACTCAAAGCCTCGtt                      >  2:1714622/1‑140 (MQ=255)                                                                                                                  aaTCGTTAAGCGAACATATGCTGGGCGTATGGTTAAAGGGAAATATCAGCCCGTCGGCGGAACTGTTTTATGAAGACCTTGATGAAGCTGATAACATTGAGGTAATCCGCAAATTGCTGGATACACTCAAAGCCTCGTTg                     >  1:305700/1‑140 (MQ=255)                                                                                                                  aaTCGTTAAGCGAACATATGCTGGGCGTATGGTTAAAGGGAAATATCAGCCCGTCGGCGGAACTGTTTTATGAAGACCTTGATGAAGCTGATAACATTGAGGTAATCCGCAAATTGCTGGATACACTCAAAGCCTCGTTg                     >  2:1396751/1‑140 (MQ=255)                                                                                                                       ttAAGCGAACATATGCTGGGCGTATGGTTAAAGGGAAATATCAGCCCGTCGGCGGAACTGTTTTATGAAGACCTTGATGAAGCTGATAACATTGAGGTAATCCGCAAATTGCTGGATACACTCAAAGCCTCGTTGATGga                >  1:335234/1‑140 (MQ=255)                                                                                                                         aaGCGAACATATGCTGGGCGTATGGTTAAAGGGAAATATCAGCCCGTCGGCGGAACTGTTTTATGAAGACCTTGATGAAGCTGATAACATTGAGGTAATCCGCAAATTGCTGGATACACTCAAAGCCTCg                        >  2:784181/1‑130 (MQ=255)                                                                                                                         aaGCGAACATATGCTGGGCGTATGGTTAAAGGGAAATATCAGCCCGTCGGCGGAACTGTTTTATGAAGACCTTGATGAAGCTGATAACATTGAGGTAATCCGCAAATTGCTGGATACACTCAAAGCCTCg                        <  1:784181/130‑1 (MQ=255)                                                                                                                         aaGCGAACATATGCTGGGCGTATGGTTAAAGGGAAATATCAGCCCGTCGGCGGAACTGTTTTATGAAGACCTTGATGAAGCTGATAACATTGAGGTAATCCGCAAATTGCTGGATACACTCAAAGCCTCGTTGATGgaag              <  2:216771/140‑1 (MQ=255)                                                                                                                              aaCATATGCTGGGCGTATGGTTAAAGGGAAATATCAGCCCGTCGGCGGAACTGTTTTATGAAGACCTTGATGAAGCTGATAACATTGAGGTAATCCGCAAATTGCTGGATACACTCAAAGCCTCGTTGATGGAAGAAAAt         >  1:1322556/1‑140 (MQ=255)                                                                                                                                     gCTGGGCGTATGGTTAAAGGGAAATATCAGCCCGTCGGCGGAACTGTTTTATGAAGACCTTGATGAAGCTGATAACATTGAGGTAATCCGCAAATTGCTGGATACACTCAAAGCCTCGTTGATGGAAGAAAATCAGATgg  >  1:1430246/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                       GGTCGCGAATCGTCTCGATTTGCCGCTGCTGCCGGTGATTTTCCCTACGCAGCTGATATTGCGCATTGAATGTCCGGATGGCGAAATTTGGCTGATTAATCCTTTTAACGGTGAATCGTTAAGCGAACATATGCTGGACGTATGGTTAAAGGGAAATATCAGCCCGTCGGCGGAACTGTTTTATGAAGACCTTGATGAAGCTGATAACATTGAGGTAATCCGCAAATTGCTGGATACACTCAAAGCCTCGTTGATGGAAGAAAATCAGATGG  >  NC_000913/1267640‑1267911

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑