Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	445,558	0	A	C	29.5%	78.7 / 22.0	44	V370G (GTA→GGA)	yajR	putative major facilitator superfamily transport protein YajR
Reads supporting (aligned to +/- strand):  ref base A (20/11);  new base C (0/13);  total (20/24)
Fisher's exact test for biased strand distribution p-value = 4.96e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

AGCAAACGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTG‑CTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAA‑CATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTACTGATAAGTGA  >  NC_000913/445420‑445694                                                                                                                                            |                                                                                                                                          aGCAAACGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTG‑CTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCCGCATTGCGCCCGCGAGAAAAAc                                                                                                                                          >  2:1614201/1‑140 (MQ=255) aGCAAACGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTG‑CTGACATACGGCGGTTCTTTCATGGTACTGGCGACCGTCCGCCACACCGCGGCCAGCATTTCGCCCGCGAGAAATAc                                                                                                                                          >  2:1444183/1‑140 (MQ=255)      aCGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTG‑CTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCt                                                                                                                                     <  1:109405/140‑1 (MQ=255)      aCGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTG‑CTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCt                                                                                                                                     <  2:162800/140‑1 (MQ=255)      aCGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTG‑CTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCt                                                                                                                                     <  1:1335478/140‑1 (MQ=255)      aCGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTG‑CTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCt                                                                                                                                     <  1:973778/140‑1 (MQ=255)      aCGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTG‑CTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCt                                                                                                                                     <  1:800567/140‑1 (MQ=255)      aCGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTG‑CTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCt                                                                                                                                     <  1:579214/140‑1 (MQ=255)       cGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTG‑CTGACATACGGCGGTTCTTTTATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTg                                                                                                                                    >  2:944890/1‑140 (MQ=255)       cGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTG‑CTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTg                                                                                                                                    <  1:949278/140‑1 (MQ=255)              ttAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTG‑CTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGGAATACCCCCTGACCGTCa                                                                                                                             <  2:1067004/140‑1 (MQ=255)                   gCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTG‑CTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAA‑CAt                                                                                                                       <  1:616171/140‑1 (MQ=255)                        gTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTG‑CTGACATACGGCGGTTCTTTTATGGTACTGGCGGCTGTCAGCCCTACTTCGGGCAGCATTGCGCCCGCGGGAAAAACCCCCCGCCCGTCAAA‑CATGCCgg                                                                                                                  >  2:1665983/1‑139 (MQ=255)                        gTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTG‑CTGACATACGGCGGTTCTTTCATTGTACTGGCGACTGTCAGCCCCACTGCGGCCAGCATTGCGCCCGCGAGAAATACCCCCTGACCGTCAAA‑CATGCCGt                                                                                                                  >  2:1312768/1‑140 (MQ=255)                        gTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTG‑CTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTTCGCCAGCGAGAAAAACCCCCCGACCGTCAAA‑CATGCCGt                                                                                                                  >  1:144504/1‑140 (MQ=255)                             cGGCAATGTTCGCCGGGATTTCAATGCGCAAACTG‑CTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAA‑CATGCCGTTAATc                                                                                                             >  1:469110/1‑140 (MQ=255)                                          ggggATTTAAATGC‑CAAACCGCCTGACATACGGGGGTTCTTTAATGTTACGGGCGACTGCCAGCCACCCTGCGGCAGGTATGGCGCAGGCAAAAAATACCCCCTGCCCGTCAAA‑CATGCCGTTAATCCAGCCGCCCAGCg                                                                                                <  2:1289841/139‑1 (MQ=255)                                             gATTTCAATGCGCAAACTG‑CTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCCGCCCCACCGCGGCCAGCATTTCGCCCGCGAGAAAAAACCCCTGACCCCCAAA‑CATGCCGTTAATCCAGCCGCCCCGCGAAc                                                                                             >  2:41984/1‑140 (MQ=255)                                              aTTTCAATGCGCAAACTG‑CTGACATACGGCGGTTCTTTCATTGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCCGCGAGAAATACCCCCCGACCGTCAAA‑CATGCCGTTAATCCAGCCGCCCAGCGAAcc                                                                                            >  2:728168/1‑140 (MQ=255)                                                ttCAATGCGCAAACTG‑CTGACATCCGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATCCCCCCTGACCGTCAAA‑CATGCCGTTAATCCAGCCGCCCAGCGAACCGc                                                                                          <  1:1444183/140‑1 (MQ=255)                                                       cgcAAACTG‑CTGACATACGGGGGTTTTTTCAGGGTACGGGCGATTGTCAGCCACACGGCGGCCAGCATTGCGCCAGCGAAAAATCCCCCCTGCCCGTCAAA‑CATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATcgc                                                                                   <  2:877753/140‑1 (MQ=255)                                                       cgcAAACTG‑CTAACATCCGGCGTTTCTTTCAGGGTACTGGCGACTGTCACCCACACTGCGGCCAGCATTGCGCCAGCGAAAAATACCCCCTGACCGTCAAA‑CATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATcgc                                                                                   <  2:1317255/140‑1 (MQ=255)                                                         caaccgg‑ctgACATACGGCGTTTTTTTCAGGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAAAAATCCCCCCTGCCCGTCAAA‑CATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATcgcca                                                                                 <  2:907279/134‑1 (MQ=255)                                                                    aCATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATCCCCCCTGACCGTCAAA‑CATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGaaa                                                                       <  1:944890/140‑1 (MQ=255)                                                                      aTACGGCGGTTCTTTCATGGTGCTGGCGACTGTCAGCCACACTGGGGCCAGCATGGCGCCGGCGAGAAATCCCCCCTGCCCGTAAAA‑CATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAAct                                                                     <  2:1360536/140‑1 (MQ=255)                                                                              gTTTTTTCATGGTACTGGCGACTGTCACCCCCACTGCGGCCAGCTTTGCGCCAGCGAGAAATCCCCCCGGCCCGTAAAA‑CATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCtggtg                                                             <  2:144504/140‑1 (MQ=255)                                                                              gTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATCCCCCCTGCCCGTCAAA‑CATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCtggtg                                                             <  1:862243/140‑1 (MQ=255)                                                                                 cTTTCATGGTACTGGCGCCTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATCCCCCCTGACCGTCAAA‑CATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAg                                                          <  1:48408/140‑1 (MQ=255)                                                                                  tttCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCCGACCGTCAAA‑CATGCCGTTAATCCAGCCGCCCCGCGAACCGCCCATCGCCACGCCAAGAAACTGGCtgggggggg                                                         >  2:862243/1‑137 (MQ=255)                                                                                        ggTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAA‑CATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAacac                                                   >  1:1376512/1‑140 (MQ=255)                                                                                         gTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAA‑CATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACAcc                                                  >  2:443383/1‑140 (MQ=255)                                                                                            cTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAA‑CATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCAt                                               >  1:100760/1‑140 (MQ=255)                                                                                            cTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAA‑CATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCAt                                               >  1:392458/1‑140 (MQ=255)                                                                                             tGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAA‑CATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATc                                              >  1:646950/1‑140 (MQ=255)                                                                                             tGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAA‑CATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATc                                              >  1:782730/1‑140 (MQ=255)                                                                                               gCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAA‑CATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGc                                            >  2:84624/1‑140 (MQ=255)                                                                                               gCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAAAAATCCCCCCTGACCGTCAAA‑CATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGc                                            <  1:638800/140‑1 (MQ=255)                                                                                                 gACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATCCCCCCTGCCCGTCAAA‑CATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCg                                          <  1:1614201/140‑1 (MQ=255)                                                                                                   cTGTCAGCCACACTGCGGCCAGCTTTGCGCCAGCGAGAAATCCCCCCTGACCGTAAAA‑CATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTa                                        <  2:1090629/140‑1 (MQ=255)                                                                                                   cTGTCAGCCACACTGCGGCCAGCATTGCGCTAGCGAGAAATCCCCCCTGACCGTAAAA‑CATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTa                                        <  1:1354772/140‑1 (MQ=255)                                                                                                    tGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAA‑CATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCg                                          <  1:1379974/137‑1 (MQ=255)                                                                                                    tGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAA‑CATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCg                                          >  2:1379974/1‑137 (MQ=255)                                                                                                    tGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAA‑CATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTAc                                       >  2:165501/1‑140 (MQ=255)                                                                                                    tGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAA‑CATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTAc                                       >  1:66665/1‑140 (MQ=255)                                                                                                    tGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAA‑CATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTAc                                       >  2:648112/1‑140 (MQ=255)                                                                                                    tGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAA‑CATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTAc                                       >  1:883004/1‑140 (MQ=255)                                                                                                                tGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAA‑CATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACctg                           >  1:1612366/1‑140 (MQ=255)                                                                                                                tGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAA‑CATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCATACCTTTGTAACctg                           >  2:1448240/1‑140 (MQ=255)                                                                                                                  gggCCAGCATTGCGCCAGCGAGAAATCCCCCCTGCCCGTCAAA‑CATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACctgct                         <  1:1224738/139‑1 (MQ=255)                                                                                                                  cGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAA‑CATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACctgct                         >  1:864231/1‑140 (MQ=255)                                                                                                                    gCCAGCATTGCGCCAGCGAGAAATCCCCCCTGACCGTCAAC‑CATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTgg                       <  2:66317/140‑1 (MQ=255)                                                                                                                    gCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAA‑CATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTgg                       >  2:4650/1‑140 (MQ=255)                                                                                                                    gCCAGCATTGCGCCAGCGAAAAATCCCCCCTGACCGTAAAA‑CATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTgg                       <  1:1312768/140‑1 (MQ=255)                                                                                                                    gCCAGCATGGCGCCAGCGAGAAATCCCCCCTGCCCGTCAAA‑CATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTgg                       <  2:1040828/140‑1 (MQ=255)                                                                                                                      cAGCTTTGCCCCAGCAAAAAATCCCCCCTGCCCGAAAAA‑CATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCg                     <  2:100760/140‑1 (MQ=255)                                                                                                                       aGCTTTGCGCCAGGGAAAAAT‑CCCCCCGACCCGCAAACCATGCCTTAAATCCACCCCCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGa                    <  2:479557/140‑1 (MQ=255)                                                                                                                       aGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAA‑CATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGa                    >  1:124444/1‑140 (MQ=255)                                                                                                                       aGCATTGCGCCAGCGAAAAATCCCCCCTGACGGTAAAA‑CTTGCCGTTAATCCAGCCGCCAAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGa                    <  2:66665/140‑1 (MQ=255)                                                                                                                         cATTGCGCCAGCGAGAAATCCCCCCTGACCGTCAAA‑CATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGAct                  <  2:58134/140‑1 (MQ=255)                                                                                                                         cATTGCGCCAGCGAAAAATCCCCCCTGACCGTCAAA‑CATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCACCGTACCTTTGTAACCTGCTGGCGAct                  <  1:1559988/140‑1 (MQ=255)                                                                                                                           ttccgcCAGCAAGAAATCCCCCCTGCCCGCCAAA‑CATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGActct                <  1:288799/137‑1 (MQ=255)                                                                                                                           ttGCGCCAGCGAGAAATCCCCCCTGACCGACAAA‑CATGCCGTAAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGActct                <  1:774517/140‑1 (MQ=255)                                                                                                                           ttGCGCCAGCGAGAAATACCCCCTGACCGTCAAA‑CATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGActct                <  1:84624/140‑1 (MQ=255)                                                                                                                           ttGCGCCAGCGAAAAATCCCCCCTGACCGTAAAA‑CATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGActct                <  1:728168/140‑1 (MQ=255)                                                                                                                             gcgcCGGCGAAAAATCCCCCCTGCCCGAAAAA‑CATGCCGTTAATCCAGCCCCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCttt              <  2:1031784/140‑1 (MQ=255)                                                                                                                             gcgcCAGGGAGAAATCCCCCCTGACCGTAAAA‑CATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCttt              <  1:890750/140‑1 (MQ=255)                                                                                                                             gcgcCAGGGAAAAAACCCCCCTGACCAGAAAA‑CACGCCGTTAATCCAGCCCCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCttt              <  2:864231/140‑1 (MQ=255)                                                                                                                                  ggCGAGAAATCCCCCTTGCCCGTAAAA‑CATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTACTGa         <  2:469110/139‑1 (MQ=255)                                                                                                                                  aGCGAGAAATCCCCCCTGACCGTCAAA‑CATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTACTGa         <  1:648112/140‑1 (MQ=255)                                                                                                                                   gCGAGAAATACCCCCTGACCGTCAAA‑CATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTACTGAt        >  1:1362342/1‑140 (MQ=255)                                                                                                                                    cGAGAAATACCCCCTGACCGTCAAA‑CATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTACTGATa       >  2:19129/1‑140 (MQ=255)                                                                                                                                       aaaaTCCCCCCTGCCCGTAAAA‑CATGCCGTTAACCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTACTGATAAGt    <  2:883004/139‑1 (MQ=255)                                                                                                                                       aaaaTCCCCCCTGACCGTCAAA‑CATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTACTGATAAGt    <  1:165501/139‑1 (MQ=255)                                                                                                                                       aaaaTCCCCCCTGACCGTCAAA‑CATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCATACCTTTGTAACCTGCTGGCGACTCTTTACTGATAAGt    <  1:1448240/139‑1 (MQ=255)                                                                                                                                         aaTCCCCCCTGCCCGTCAAC‑CATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTACTGATAAGTGa  <  1:41984/140‑1 (MQ=255)                                                                                                                                            |                                                                                                                                          AGCAAACGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTG‑CTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAA‑CATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTACTGATAAGTGA  >  NC_000913/445420‑445694

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑