| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 786,855 | 0 | A | C | 26.0% | 108.1 / 15.8 | 50 | G247G (GGT→GGG) | gpmA | 2,3‑bisphosphoglycerate‑dependent phosphoglycerate mutase |
| Reads supporting (aligned to +/- strand): ref base A (30/7); new base C (0/13); total (30/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.18e-07 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GCGTATCCGATGAATCACCACAGGCCTGATAAGTCGCGCAGCGTCGCATCAGGCAATGTGCTCCATTGTTAGCAACAAAAAAGCCGACTCACTTGCAGTCGGCTTTCTCATTTTAAACGAATGACGTTTACTTCGCTTTACCCTGGTTTGCAACCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCTCGTCGAACTCATACACCAGCGGCACGCCAGTCGGGATATTAAGCTCAAGAA > NC_000913/786716‑786988 | gCGTATCCGATGAATCACCACAGGCCTGATAAGTCGCGCAGCGTCGCATCAGGCAATGTGCTCCATTGTTAGCAACAAAAAAGCCGACTCACTTGCAGTCGGCTTTCTCATTTTAAACGAATGACGTTTACTTCGCTTTa > 1:535242/1‑140 (MQ=255) tATCCGATGAATCACCACAGGCCTGATAAGTCGCGCAGCGTCGCATCAGGCAATGTGCTCCATTGTTAGCAACAAAAAAGCCGACTCACTTGCAGTCGGCTTTCTCATTTTAAACGAATGACGTTTACTTCGCTTTAccc < 2:722331/140‑1 (MQ=255) ccGATGAATCACCACAGGCCTGATAAGTCGCGCAGCGTCGCATCAGGCAATGTGCTCCATTGTTAGCAACAAAAAAGCCGACTCACTTGCAGTCGGCTTTCTCATTTTAAACGAATGACGTTTACTTCGCTTTACCCTgg > 2:289070/1‑140 (MQ=255) cGATGAATCACCACAGGCCTGATAAGTCGCGCAGCGTCGCATCAGGCAATGTGCTCCATTGTTAGCAACAAAAAAGCCGACTCACTTGCAGTCGGCTTTCTCATTTTAAACGAATGACGTTTACTTCGCTTTACCCTGGt > 1:1257706/1‑140 (MQ=255) caGGCCTGATAAGTCGCGCAGCGTCGCATCAGGCAATGTGCTCCATTGTTAGCAACAAAAAAGCCGACTCACTTGCAGTCGGCTTTCTCATTTTAAACGAATGACGTTTACTTCGCTTTACCCTGGTTTGCAACcgccgc > 1:374183/1‑140 (MQ=255) ccTGATAAGTCGCGCAGCGTCGCATCAGGCAATGTGCTCCATTGTTAGCAACAAAAAAGCCGACTCACTTGCAGTCGGCTTTCTCATTTTAAACGAATGACGTTTACTTCGCTTTACCCTGGTTTGCAACCGCCgctgct > 2:881898/1‑140 (MQ=255) aTAAGTCGCGCAGCGTCGCATCAGGCAATGTGCTCCATTGTTAGCAACAAAAAAGCCGACTCACTTGCAGTCGGCTTTCTCATTTTAAACGAATGACGTTTACTTCGCTTTACCCTGGTTTGCAACCGCCGCTGCTTTCg > 1:1055905/1‑140 (MQ=255) aTAAGTCGCGCAGCGTCGCATCAGGCAATGTGCTCCATTGTTAGCAACAAAAAAGCCGACTCACTTGCAGTCGGCTTTCTCATTTTAAACGAATGACGTTTACTTCGCTTTACCCTGGTTTGCAACCGCCGCTGCTTTCg > 2:955546/1‑140 (MQ=255) tCGCGCAGCGTCGCATCAGGCAATGTGCTCCATTGTTAGCAACAAAAAAGCCGACTCACTTGCAGTCGGCTTTCTCATTTTAAACGAATGACGTTTACTTCGCTTTACCCTGGTTTGCAACCGCCGCTGCTTTCGCTGCg > 2:1635380/1‑140 (MQ=255) cgcgCAGCGTCGCATCAGGCAATGTGCTCCATTGTTAGCAACAAAAAAGCCGACTCACTTGCAGTCGGCTTTCTCATTTTAAACGAATGACGTTTACTTCGCTTTACCCTGGTTTGCAACCGCCGCTGCTTTCGCTGCGa > 1:1258803/1‑140 (MQ=255) gcgcAGCGTCGCATCAGGCAATGTGCTCCATTGTTAGCAACAAAAAAGCCGACTCACTTGCAGTCGGCTTTCTCATTTTAAACGAATGACGTTTACTTCGCTTTCCCCTGTTTTGCAACCGCCGCTGCTTTCGCTGCGAt < 2:535242/140‑1 (MQ=255) gcgcAGCGTCGCATCAGGCAATGTGCTCCATTGTTAGCAACAAAAAAGCCGACTCACTTGCAGTCGGCTTTCTCATTTAAAACGAATGACGTTTACTTCGCTTTCCCCTGGTTTGCAACCGCCGCTGCTTTCGCTGCGAt < 1:470886/140‑1 (MQ=255) cgcAGCGTCGCATCAGGCAATGTGCTCCATTGTTAGCAACAAAAAAGCCGACTCACTTGCAGTCGGCTTTCTCATTTTAAACGAATGACGTTTACTTCGCTTTACCCTGGTTTGCAACCGCCGCTGCTTTCGCTGCGAtc > 2:1210262/1‑140 (MQ=255) gcAGCGTCGCATCAGGCAATGTGCTCCATTGTTAGCAACAAAAAAGCCGACTCACTTGCAGTCGGCTTTCTCATTTTAAACGAATGACGTTTACTTCGCTTTACCCTGGTTTGCAACCGCCGCTGCTTTCGCTGCGAtct > 2:25309/1‑140 (MQ=255) gCGTCGCATCAGGCAATGTGCTCCATTGTTAGCAACAAAAAAGCCGACTCACTTGCAGTCGGTTTTCTCATTTAAAACGAATGACTTTTCCTTCGCTTTCCCCTGGTTTGCAACCGCCGCTGCTTTCGCTGCGATCTCGt < 2:381143/140‑1 (MQ=255) tCGCATCAGGCAATGTGCTCCATTGTTAGCAACAAAAAAGCCGACTCACTTGCAGTCGGCTTTCTCATTTTAAACGAATGACGTTTACTTCGCTTTACCCTGGTTTGCAACCGCCGCTGCTTTCGCTGCGATCTCGTCAg > 1:1544253/1‑140 (MQ=255) gCATCAGGCAATGTGCTCCATTGTTAGCAAAAAAAAAGCCGCCTCCCTTGCGGTCGGTTTTCTCTTTTTAAACGAATGACTTTTCCTTCGTTTTCCCCTGTTTTGCAACCGCCGCTGCTTTCGCTGCGATCTCGTCAGCa < 2:92086/140‑1 (MQ=255) gCATCAGGCAATGTCCTCCATTGTTAGAAAAAAAAAACCCGACTCACTTGCAGCCGGCTTTCTCATTTAAAACGAATGACTTTTCCTTCGCTTTCCCCTGTTTTGCAACCGCCGCTGCTTTCGCTGCGATCTCGTCAGCa < 2:1165132/140‑1 (MQ=255) ccaccAGGCAATGTGCTCCATTTTGAGAAAAAAAAAAGCCGACTCATTTGCAGGCGGCTTTCTTTTTTAAACCGAATGGCTTTTCCTTCGCTTTACCCTGTTTTGCACCCGCCGCTGCTTTCGCTGCGATCTCGTCAGCa < 2:1513641/136‑1 (MQ=255) aTCAGGCAATGTGCTCCATTGTTAGCAACAAAAAAGCCGCCTCACTTGCAGTCGGTTTTCTCATTTTAAACGAATGCCTTTTACTTCGCTTTCCCCTGGTTTGCAACCGCCGCTGCTTTCGCTGCGATCTCGTCAGCAtt < 2:460871/140‑1 (MQ=255) gCAATGTGCTCCATTGTTAGCAACAAAAAAGCCGACTCACTTGCAGTCGGCTTTCTCATTTTAAACGAATGACGTTTACTTCGCTTTCCCCTGGTTTGCAACCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCa < 1:1145009/140‑1 (MQ=255) tgtgCTCCATTGTTAGCAACAAAAAAGCCGACTCACTTGCAGTCGGCTTTCTCATTTTAAACGAATGACGTTTACTTCGCTTTACCCTGGTTTGCAACCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGata > 2:26179/1‑140 (MQ=255) tCCATTGTTAGCAACAAAAAAGCCGACTCACTTGCAGTCGGCTTTCTCATTTTAAACGAATGACGTTTACTTCGCTTTACCCTGGTTTGCAACCGCTGCTGCTTTCGCTGCGATCTCGTCAGCa < 2:407364/124‑1 (MQ=255) tCCATTGTTAGCAACAAAAAAGCCGACTCACTTGCAGTCGGCTTTCTCATTTTAAACGAATGACGTTTACTTCGCTTTACCCTGGTTTGCAACCGCTGCTGCTTTCGCTGCGATCTCGTCAGCa > 1:407364/1‑124 (MQ=255) tGTTAGCAACAAAAAAGCCGACTCACTTGCAGTCGGCTTTCTCATTTTAAACGAATGACGTTTACTTCGCTTTACCCTGGTTTGCAACCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTc > 1:219432/1‑140 (MQ=255) tGTTAGCAACAAAAAAGCCGACTCACTTGCAGTCGGCTTTCTCATTTTAAACGAATGACGTTTACTTCGCTTTACCCTGGTTTGCAACCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTc > 1:895574/1‑140 (MQ=255) ttAGCAACAAAAAAGCCGACTCACTTGCAGTCGGCTTTCTCATTTTAAACGAATGACGTTTACTTCGCTTTACCCTGGTTTGCAACCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAg > 1:1098527/1‑140 (MQ=255) aGCAACAAAAAAGCCGACTCACTTGCAGTCGGCTTTCTCATTTTAAACGAATGACGTTTACTTCGCTTTACCCTGGTTTGCAACCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCg > 2:937243/1‑140 (MQ=255) aacaaAAAAGCCGACTCACTTGCAGTCGGCTTTCTCATTTTAAACGAATGACGTTTACTTCGCTTTACCCTGGTTTGCAACCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGtt > 2:651542/1‑140 (MQ=255) caaAAAAGCCGACTCACTTGCAGTCGGCTTTCTCATTTTAAACGAATGACGTTTACTTCGCTTTACCCTGGTTTGCAACCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTg > 1:450179/1‑140 (MQ=255) caaAAAAGCCGACTCACTTGCAGTCGGCTTTCTCATTTTAAACGAATGACGTTTACTTCGCTTTACCCTGGTTTGCAACCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTg > 2:226251/1‑140 (MQ=255) gACTCACTTGCAGTCGGTTTTCTCATTTTAAACGAATGACGTTTACTTCGCTTTCCCCTGGTTTGCAACCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCtcgt < 1:163556/140‑1 (MQ=255) aaCTCTCTTGCAGTCGGTTTTCTCTTTTTAAACGAATGACGTTTACTTCCTTTTCCCCTGGTTTGCAACCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCtcgt < 1:289070/139‑1 (MQ=255) cTCACTTGCAGTCGGTTTTCTCTTTTTAAACGAATGACGTTTCCTTCGCTTTCCCCTGGTTTGCAACCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCtcgtcg < 2:1258803/140‑1 (MQ=255) cTCACTTGCAGTCGGCTTTCTCATTTTAAACGAATGACGTTTACTTCGCTTTCCCCTGGTTTGCAACCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCtcgtcg < 1:1032855/140‑1 (MQ=255) tCACTTGCAGTCGGCTTTCTCATTTTAAACGAATGACGTTTACTTCGCTTTCCCCTGGTTTGCAACCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCTCGTCGa < 1:25309/140‑1 (MQ=255) tCACTTGCAGTCGGCTTTCTCATTTAAAACGAATGACGTTTACTTCGCTTTCCCCTGGTTTGCAACCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCTCGTCGa < 2:450179/140‑1 (MQ=255) tcTTGCAGTCGGCTTTCTCATTTTAAACGAATGACTTTTCCTTCGCTTTCCCTTGGTTTGCAGCCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCTCGTCGAAc < 2:20818/139‑1 (MQ=255) tGCAGTCGGCTTTCTCATTTTAAACGAATGACTTTTACTTCGCTTTCCCCTGTTTTGCAACCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCTCGTCGAACTCa < 1:1359799/140‑1 (MQ=255) tGCAGTCGGCTTTCTCATTTTAAACGAATGACGTTTACTTCGCTTTACCCTGGTTTGCAACCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCTCGTCGAACTCa > 1:1631442/1‑140 (MQ=255) tCGGCTTTCTCATTTTAAACGAATGACGTTTACTTCGCTTTACCCTGGTTTGCAACCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCTCGTCGAACTCATacac > 1:1705283/1‑140 (MQ=255) ggCTTTCTCATTTTAAACGGATGACGTTTACTTCGCTTTACCCTGGTTTGCAACCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCTCGTCGAACTCATACACCa > 1:231114/1‑140 (MQ=255) ggCTTTCTCATTTTAAACGAATGACGTTTACTTCGCTTTACCCTGGTTTGCAACCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCTCGTCGAACTCATACACCa > 1:20818/1‑140 (MQ=255) ggCTTTCTCATTTTAAACGAATGACGTTTACTTCGCTTTACCCTGGTTTGCAACCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCTCGTCGAACTCATACACCa > 1:369442/1‑140 (MQ=255) ggCTTTCTCATTTTAAACGAATGACGTTTACTTCGCTTTACCCTGGTTTGCAACCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCTCGTCGAACTCATACACCa > 1:1370369/1‑140 (MQ=255) gCTTTCTCATTTTAAACGAATGACGTTTACTTCGCTTTACCCTGGTTTGCAACCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCTCGTCGAACTCATACACCAg > 1:447748/1‑140 (MQ=255) cTTTCTCATTTTAAACGAATGACGTTTACTTCGCTTTACCCTGGTTTGCAACCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCTCGTCGAACTCATGCACCAGc > 2:1123563/1‑140 (MQ=255) cttaTTTTAAACGAATGACTTTTTCTTCGCTTTCCCCTGGTTTGCAACCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCTCGTCGAACTCATACACCAGCGGCa < 2:374183/137‑1 (MQ=255) taTTTTAACCGAATGACTTTTACTTCGCTTTCCCCTGGTTTGCAACCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCTCGTCGAACTCATACACCAGCGGCACg < 2:895574/139‑1 (MQ=255) ttAAACGAATGACTTTTCCTTCGCTTTCCCCTGTTTTGCAACCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCTCGTCGAACTCATACACCAGCGGCACGCCAg < 2:1055905/140‑1 (MQ=255) taaaaCGAATGACGTTTACTCCGCTTTCCCCTGGTTTGCAACCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCTCGTCGAACTCATACACCAGCGGCACGCCAg < 1:861771/138‑1 (MQ=255) aCGAATGACTTTTCCTTCGCTTTCCCCTGTTTTCCAACCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCTCGTCGAACTCATACACCAGCGGCACGCCAGTCgg < 2:1370369/140‑1 (MQ=255) aCGAATGACGTTTACTTCGCTTTACCCTGGTTTGCAACCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCTCGTCGAACTCATACACCAGCGGCACGCCAGTCgg < 2:447748/140‑1 (MQ=255) gACGTTTACTTCGCTTTACCCTGGTTTGCAACCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCTCGTCGAACTCATACACCAGCGGCACGCCAGTCGGGATAtt < 2:231114/140‑1 (MQ=255) ctttttCTTCGTTTTCCCCTGGTTTGCACCCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCTCGTCGAACTCATACACCAGCGGCACGCCAGTCGGGATATTaa < 2:1544253/138‑1 (MQ=255) gTTTACTTCGCTTTACCCTGGTTTGCAACCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCTCGTCGAACTCATACACCAGCGGCACGCCAGTCGGGATATTAAg < 1:226251/140‑1 (MQ=255) gTTTACTTCGCTTTACCCTGGTTTGCAACCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCTCGTCGAACTCATACACCAGCGGCACGCCAGTCGGGATATTAAg < 1:1635380/140‑1 (MQ=255) ttACTTCGCTTTCCCCTGGTTTGCAACCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCTCGTCGAACTCATACACCAGCGGCACGCCAGTCGGGATATTAAGCt < 2:180726/140‑1 (MQ=255) ccTTCGCTTTCCCCTGTTTTGCAACCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCTCGTCGAACTCATACACCAGCGGCACGCCAGTCGGGATATTAAGCTCa < 1:852969/139‑1 (MQ=255) cGCTTTACCCTGGTTTGCAACCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCTCGTCGAACTCATACACCAGCGGCACGCCAGTCGGGATATTAAGCTCAAGaa > 1:871267/1‑140 (MQ=255) gCTTTACCCTGGTTTGCAACCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCTCGTCGAACTCATACACCAGCGGCACGCCAGTCggg < 2:1489245/123‑1 (MQ=255) gCTTTACCCTGGTTTGCAACCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCTCGTCGAACTCATACACCAGCGGCACGCCAGTCggg > 1:1489245/1‑123 (MQ=255) | GCGTATCCGATGAATCACCACAGGCCTGATAAGTCGCGCAGCGTCGCATCAGGCAATGTGCTCCATTGTTAGCAACAAAAAAGCCGACTCACTTGCAGTCGGCTTTCTCATTTTAAACGAATGACGTTTACTTCGCTTTACCCTGGTTTGCAACCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCTCGTCGAACTCATACACCAGCGGCACGCCAGTCGGGATATTAAGCTCAAGAA > NC_000913/786716‑786988 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |