Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,604,3440CT48.1% 70.9 / 98.7 108S62F (TCT→TTT) bglJDNA‑binding transcriptional regulator BglJ
Reads supporting (aligned to +/- strand):  ref base C (8/48);  new base T (52/0);  total (60/48)
Fisher's exact test for biased strand distribution p-value = 1.22e-22
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 130 total aligned reads displayed.

ATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCT‑GACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCAT  >  NC_000913/4604207‑4604481
                                                                                                                                          |                                                                                                                                          
aTGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGGGCTtttttttt                                                                                                                                          >  2:1059577/1‑140 (MQ=255)
  gCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATtttttctttt                                                                                                                                        <  1:1386803/140‑1 (MQ=255)
   cGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATtttttcttttt                                                                                                                                       <  1:335978/140‑1 (MQ=255)
     tCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTttttctttttct                                                                                                                                     <  2:273438/140‑1 (MQ=255)
     tCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTttttctttttct                                                                                                                                     <  1:594941/140‑1 (MQ=255)
       aTGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTCTTTTTCTGc                                                                                                                                   <  2:313494/140‑1 (MQ=255)
              gtaTCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTTTTTTTCTGCCATga                                                                                                                              >  1:87167/1‑138 (MQ=255)
              gtaTCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTCTTTTTCTGCCATga                                                                                                                              <  2:87167/138‑1 (MQ=255)
               taTCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCCTTTTTTTTTTTTCTGCCCTGAGaa                                                                                                                           >  1:1220657/1‑140 (MQ=255)
                aTCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTTTTTTTTTTCCATGAGaaa                                                                                                                          >  2:1667818/1‑139 (MQ=255)
                 tCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTCTTTTTCTGCCATGAGAAGt                                                                                                                         <  1:1667818/140‑1 (MQ=255)
                    gTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAg                                                                                                                      >  1:42408/1‑140 (MQ=255)
                      aTTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTGGTTTTTTTTTTTTTTGGCGTGCAAAGTGAgcc                                                                                                                    >  1:1692398/1‑139 (MQ=255)
                      aTTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTTTTTTTTTGCCATGAGAAGTGAggg                                                                                                                    >  2:1062341/1‑138 (MQ=255)
                      aTTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAgcg                                                                                                                    <  2:1164729/140‑1 (MQ=255)
                      aTTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAgcg                                                                                                                    <  2:27183/140‑1 (MQ=255)
                        tGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTTTTTTTCTGCCATGAGAAGTGAGCGCa                                                                                                                  >  1:254708/1‑140 (MQ=255)
                        tGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCa                                                                                                                  <  1:1062341/140‑1 (MQ=255)
                         gagaGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTTTTTTTCTGCCATGAGAAGTGAGCGCag                                                                                                                 >  2:450117/1‑140 (MQ=255)
                         gagaGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTCTTTTTCTGc                                                                                                                                   <  1:962180/122‑1 (MQ=255)
                         gagaGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTCTTTTTCTGc                                                                                                                                   >  2:962180/1‑122 (MQ=255)
                         gagaGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCag                                                                                                                 >  1:1191918/1‑140 (MQ=255)
                          agagTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGGCATTTTTTTTTTTTCTGCCCTGAGAAGGGAgcgccgg                                                                                                                >  2:913854/1‑137 (MQ=255)
                           gagTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCagag                                                                                                               <  1:1082387/140‑1 (MQ=255)
                           gagTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCagag                                                                                                               <  1:738227/140‑1 (MQ=255)
                            agTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTTTTTTTTTTCCATTAGAAGTGAGCGCagaga                                                                                                              >  1:820574/1‑140 (MQ=255)
                            agTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTTTTTTTTTGCCATGAGAAGTGAGCGCagaga                                                                                                              >  1:736173/1‑140 (MQ=255)
                            agTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTTTTTTTTTGCCATGAGAAGGGAGCGCcgagg                                                                                                              >  1:1571718/1‑139 (MQ=255)
                            agTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTTTTTTTCTTCCATGAGGAGTGAGCGCagaga                                                                                                              >  2:1082387/1‑140 (MQ=255)
                               ttATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTTTTTTTTTTCCCTGAGAAGTGAGCGCAGAGgggg                                                                                                           >  2:215331/1‑140 (MQ=255)
                               ttATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTTTTTTTTTTCCCTGAGAAGTGAGCGCAGAGAggg                                                                                                           >  2:620690/1‑140 (MQ=255)
                                 aTTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGAt                                                                                                         <  2:623205/140‑1 (MQ=255)
                                    cAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTAt                                                                                                      <  1:520835/140‑1 (MQ=255)
                                     aGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATc                                                                                                     <  1:1476315/140‑1 (MQ=255)
                                      gAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTTTTTTTTTTCCCTGAGAAGTGGGCGCAGAGgggggtatttt                                                                                                    >  2:1004471/1‑133 (MQ=255)
                                      gAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTTTTTTTTTTCCCTGAGAAGGGAGCGCAGGGAGGGATTAttt                                                                                                    >  2:520835/1‑138 (MQ=255)
                                            ttGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCct‑g                                                                                             >  1:1498732/1‑140 (MQ=255)
                                                     aCCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGAGCTTTTTTTTTTTTCTTCCATGTGAAGGGGGCGCCGCGAGGGGGTAACTTGCCT‑CATTGAAATg                                                                                    >  2:1002652/1‑140 (MQ=255)
                                                           ataAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCT‑GACTGAACTGGCGAtt                                                                              <  1:1681039/140‑1 (MQ=255)
                                                               gCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCT‑GACTGAACTGGCGATTAAGt                                                                          <  2:1227274/140‑1 (MQ=255)
                                                                cTCCATACCTATACCAGTCAGGAGTCATTTCAGGACGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTTTTTTTTTTCCCTGAGAAGGGAGCGCCGAGAGGGGTTATCTTGTCT‑GACTGAAATGGCGATTAAGtt                                                                         >  2:1638229/1‑140 (MQ=255)
                                                                 tCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTTTTTTTTTGCCCTGAGAAATGAGGGCGGGGGGGGGTTTTCTTGCCTGGCCGGAACTGGGGTTAAAGtt                                                                         >  2:354351/1‑140 (MQ=255)
                                                                 tCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTCTTTTTCTGCCCTGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCT‑GACTGAACTGGCGATTAAAttt                                                                        >  1:868428/1‑140 (MQ=255)
                                                                  ccATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTTTTTTTCTTCCATGAGAAGGGAGCGCAGAGAGGGGTTATCTTGCCT‑GACTGAACTGGCGGtttaaattt                                                                       >  2:772381/1‑133 (MQ=255)
                                                                   cATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTTTTTTTTTGCCCTGAGAAGTGAGCGCAGAGAGGGGTTATCTTGCCT‑GACTGAACTGGCGGTTAAGgtttc                                                                      >  2:340888/1‑138 (MQ=255)
                                                                     tACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTTTTTTTTTGCCATGAGAAGTGAGCGCAGAGAGGGATTATTTTTCCT‑GACTGAACTGGGGATTAAGTTTTcgc                                                                    >  2:1241535/1‑140 (MQ=255)
                                                                          ataCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTTTTTTTCTGCCCTGAGAAGGGAAGGCCGAGAGGGGTTATCTTGTCT‑TACTTAACTGGGGGTTAAGTTTCCTGGTAcc                                                               >  2:666171/1‑140 (MQ=255)
                                                                           taCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCCTTTTTTTTTTTTTTTCCCTGAGAAGGGAGGGCCGAGAGGGGTTATTTTTTCT‑GACTGAACTGGGGGTTAAATTTTCGCGTAccc                                                              >  2:837723/1‑140 (MQ=255)
                                                                            aCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCT‑GACTGAACTGGCGATTAAGTTTCCGCGTACCCg                                                             <  1:663378/140‑1 (MQ=255)
                                                                               aGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCT‑GACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCg                                                          <  1:1636383/140‑1 (MQ=255)
                                                                                gTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTTTTTTTTTTCCATGAGGAGTGAGGGCAGAGAGGGGTTATCTTTCCT‑GACTGGACTGGGGGTTTAATTTTCGCGGACCCggggg                                                         >  2:1439276/1‑137 (MQ=255)
                                                                                gTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTTTTTTTTTTCCATGAGAAGGGAGCGCAGAGAGGGGTTATCTTGCCT‑GACTGAACTGGCGATTAAGGTTCCGCGGACCCGGCgg                                                         >  2:909476/1‑139 (MQ=255)
                                                                                gTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTTTTTTTTTGCCCTCAGTAGGGGGAGCAGCGAGGGGTTTTTTTGCCT‑TACTGAACTGGCGGTTAAATTTTCGCGCAACCGGCgg                                                         >  2:1064015/1‑139 (MQ=255)
                                                                                gTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCT‑GACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGt                                                         <  2:254708/140‑1 (MQ=255)
                                                                                gTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GG‑CATTTTTTTTTTTTTTTCCATGAGAAGTCAGCGCAGAGAGGGAGTATCTTGCCT‑TGCTGAAATGGCGGTTAAGGTTCCGCGGACACGGCggt                                                        >  2:1068043/1‑138 (MQ=255)
                                                                                 tCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTTTTTTTTTGCCCTGAGAAGTGAGCGCAGAGAGGGGGTATCTTTCCT‑GACTGAACTGGGGATTAAGTTTTCGCGTACCCGGCggt                                                        >  2:1636383/1‑138 (MQ=255)
                                                                                 tCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTTTTTTTTTGCCATGAGAAGTGAGCGCAGGGGGGGGTTATCTTTCCT‑GACTGAACTGGCGGTTAAGTTTTCGCGTACCCGGCggt                                                        >  2:160531/1‑138 (MQ=255)
                                                                                 tCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTTTTTTTTTGCCATGAGAAGTGAGCGCAGAGGGGGGTTATCTTGCCT‑GACTGAACTGGCGATTAAGATTCCGCGTACCCGGCggt                                                        >  2:1196304/1‑138 (MQ=255)
                                                                                 tCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTTTTTTTTTGCCATGAGAAGTGAGCGCAGAGAGGGGTTATCTTTCCT‑GACTGAACTGGCGATTAAGTTTTCGCGGACCCGGCggt                                                        >  2:1397153/1‑138 (MQ=255)
                                                                                 tCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTTTTTTTTTGCCATGAGAAGGGAGCGCAGAGAGGGGTTATCTTGCCT‑GACTGAACTGGCGATTAAGTTTTCGCGTACCCGGCGtt                                                        >  2:475445/1‑140 (MQ=255)
                                                                                 tCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTTTTTTTCTTCCCTGAGAAGGGAGCGCCGGGAGGGGTTATTTTTCCT‑GACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCggt                                                        >  2:801531/1‑138 (MQ=255)
                                                                                 tCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTTTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGGTTATCTTGCCT‑GACTGAACTGGCGATTAAATTTTCGCGTACCCGGCggt                                                        >  2:134031/1‑138 (MQ=255)
                                                                                 tCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCT‑GACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGtt                                                        <  2:251333/140‑1 (MQ=255)
                                                                                 tCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGAGATTTTTTTTTTTTCTGCCCTGCGAAAATAGAGGAAGGAGGGGGTATCTTGCCT‑TATTGTACGGGCGATTTAGGTTTCGCGTCCCCGGCggt                                                        >  2:924915/1‑138 (MQ=255)
                                                                                  cAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTTTTTTTTTGCCATGAGAAGGGAGCGCAGGGGGGGGTTATCTTTCCT‑GACTGAACTGGCGGTTAAGGTTTCGCGGACCCGGCGgtt                                                       >  2:1461750/1‑140 (MQ=255)
                                                                                  cAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCT‑GACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGttt                                                       <  1:1461750/140‑1 (MQ=255)
                                                                                     gAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCCTTTTTTTTTTTTCTGCCATGAGAAGTGGGGGCAGAGAGGGGGTATTTTGCCT‑GACTGAACTGGCGGTTAAGGTTTCGCGTACCCGGCGGTTAGt                                                    >  2:250817/1‑140 (MQ=255)
                                                                                     gAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTTTTTTTTTTCCCTGAGAAGTGAGCGCAGGGAGGGGGTATCTTGCCT‑GACTGAACTGGCGGTTAAGTTTTCGCGTACCCCGCGTTTTGt                                                    >  2:1711074/1‑140 (MQ=255)
                                                                                     gAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCT‑GACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGt                                                    <  1:594388/140‑1 (MQ=255)
                                                                                      aGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTTTTTTTTTGCCCTGAGGAGTGAGCGCAGAGAGGGGTTATCTTTCCT‑GACTGAACTGGCGATTAAGTTTTCGCGTACCCCGCGGTTAGtt                                                   >  2:663378/1‑140 (MQ=255)
                                                                                           tttCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCT‑GACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGc                                              >  1:1504567/1‑140 (MQ=255)
                                                                                            ttCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTCTTTTTTTGCCCTGAGAAGTGAGCGCCGAGGGGGATTTTTTTGCCC‑TTCTTAACGGGGGGTTAAGTTTTCCCGTACCCCGCGTTTAGCTTTTgag                                             >  2:1027027/1‑138 (MQ=255)
                                                                                            ttCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCT‑GACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCg                                             <  1:27250/140‑1 (MQ=255)
                                                                                            ttCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCT‑GACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCg                                             <  2:1498732/140‑1 (MQ=255)
                                                                                              cAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCT‑GACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGga                                           <  1:1119553/140‑1 (MQ=255)
                                                                                               aGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCT‑GACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGgat                                          <  1:134031/140‑1 (MQ=255)
                                                                                                ggATGCCATGTCGCGGATCTCGTTTGCGGCGGGATATTTTTTTTTTTTTTGCC‑TAAGAAGTCGGCGCAGAGATGGGACATCCTGCCT‑GAACTCACTGGCGGCTAAGTTTGCGCGTCCCCCGGGTTTTGTttttttgggtg                                         >  2:557929/1‑133 (MQ=255)
                                                                                                ggATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTTTTTTTCTGCCATGAGAAGTGAGCGCAGAGGGGGATTATCTTGCCT‑GACTGAACTGGCGATTAAATTTCCGCGTACCCGGCGTTTTGTTATTGCGggtg                                         >  2:1094725/1‑140 (MQ=255)
                                                                                                 gacgCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCT‑GACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGgatga                                        <  1:1638229/137‑1 (MQ=255)
                                                                                                 gATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCT‑GACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGgatga                                        <  1:909476/140‑1 (MQ=255)
                                                                                                 gATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTCTTTTTCTACCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCT‑GACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGgatga                                        <  1:1059577/140‑1 (MQ=255)
                                                                                                  aTGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCT‑GACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGgatgat                                       <  2:659763/140‑1 (MQ=255)
                                                                                                   tGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCT‑GACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGgatgatg                                      <  1:402244/140‑1 (MQ=255)
                                                                                                      cATGTCGCGGATCTCGTTTGCGGC‑GGTCCTTTTTTTTTTTTTTTCCCTGAGAAGTGAGCGCAGAGAGGGGTTATTTTTCCT‑GACTGAACTTGCGGTTTAATTTTCGCGTACCCCGCGGTTTGTTATTTCGGGTGATGata                                   >  2:468888/1‑140 (MQ=255)
                                                                                                      cATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTTTTTTTTTTCCATGAGAAGGGGGCGCCGAGAGGGGTTATTTTGCCT‑GACTGAACTGGCGATTTAGTTTTCGCGGACCCCGCGGTTTGTTATTTTGGGTGATGata                                   >  2:242841/1‑140 (MQ=255)
                                                                                                       aTGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCT‑GACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGatat                                  <  1:268129/140‑1 (MQ=255)
                                                                                                        tGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCT‑GACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATAtt                                 <  2:1174677/140‑1 (MQ=255)
                                                                                                         gTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTTTTTTTTTTGCATTAGAAGGGAGCGCAGAGGGGGGTTATTTTGCCT‑GTCTTAA‑TGGCGAATTAATTTTCGGGGACCCGGCGTTTTGTTAATTCGGGAGGTGATAttta                               >  2:419154/1‑138 (MQ=255)
                                                                                                              ggATCTCGTTTGCGGC‑GGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCT‑GACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCt                           <  1:1027027/140‑1 (MQ=255)
                                                                                                                  ctcGTTTGCGGC‑GGTCCTTTTTTTTTTTTTTTCCCTGAGAAGTGAGGGCAGAGAGGGGTTATCTTTCCT‑GACTGAACTGGCGATTTAGTTTTCGCGTACCCGGCGGTTTGTTATTTCGGAGGATGATATTTAAACTCGGc                       >  2:1270345/1‑140 (MQ=255)
                                                                                                                    cGTTTGCGGC‑GGTCATTTTTTTTTTTTTTGCCCTGAGAAGTGAGCGCAGAGGGGGGTTATCTTGCCT‑GTCTGAACTGGCGGTTAAGTTTTCGCGGACCCCGCGTTTTGTTATTTGGGATGATGATATTTTAGCTCGGCTg                     >  2:1370044/1‑140 (MQ=255)
                                                                                                                     gTTTGCGGC‑GGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCT‑GACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGa                    <  1:1370044/140‑1 (MQ=255)
                                                                                                                          cggc‑ggTCATTTTTTTTTTTTTTGCCCTGGGAAGGGAGCGCCGAGAGGGGTTATCTTGCCT‑TACTGAACTGGCGATTAAGTTTTCCCGTACCCCGCGGTTTGTTATTTCGGATGATGATATTTAAGCTCGGCtggttggt               >  2:992397/1‑140 (MQ=255)
                                                                                                                             c‑ggTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCT‑GACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCa            <  2:1698969/140‑1 (MQ=255)
                                                                                                                                gTCATTTTTTTTTTTTTTTCCCTGAGAAGGGAGCGCAGAGAGGGGTTATCTTGCCT‑GACTGAACTGGCGATTAAGTTTCCGCGGACCCCGCGGTTTGTTATTGCGGGTGATGATATTTAAGCTCGGCTGATTTGTTTAtt          >  2:1146947/1‑140 (MQ=255)
                                                                                                                                       tttctttttctGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCT‑GACTGAA‑TGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCAt  <  1:419154/140‑1 (MQ=255)
                                                                                                                                        ttctttttctGCCATGAGAAGTGATCGCAGAGAGGGATTATCTTGCCT‑GACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCAt  <  2:794043/140‑1 (MQ=255)
                                                                                                                                        ttctttttctGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCT‑GACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCAt  <  2:1504567/140‑1 (MQ=255)
                                                                                                                                        ttctttttctGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCT‑GACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCAt  <  2:1564788/140‑1 (MQ=255)
                                                                                                                                        ttctttttctGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCT‑GACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCAt  <  2:1571718/140‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                          
ATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGC‑GGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCT‑GACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCAT  >  NC_000913/4604207‑4604481

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑