Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	354,503	G→A	A600T (GCG→ACG)	prpE →	propionyl‑CoA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	354,503	0	G	A	100.0%	293.6 / NA	82	A600T (GCG→ACG)	prpE	propionyl‑CoA synthetase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (29/53);  total (29/53)

GCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCGGCGCACGTCTGGTTTGTCTCGCAATTGCCAAAAACGCGATCCGGAAAAATGCTGCGCCGCACGATCCAGGCGATTTGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTG  >  NC_000913/354368‑354632                                                                                                                                        |                                                                                                                                  gcgcACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCGGCGCACGTCTGGTTTGTCTCGCAATTGCCAAAAACGCGATCCGGAAAAATGCTGCGCCGCACGATCCAGACGAt                                                                                                                               >  1:506178/1‑140 (MQ=255) gcgcACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCGGCGCACGTCTGGTTTGTCTCGCAATTGCCAAAAACGCGATCCGGAAAAATGCTGCGCCGCACGATCCAGACGAt                                                                                                                               >  2:54680/1‑140 (MQ=255) gcgcACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCGGCGCACGTCTGGTTTGTCTCGCAATTGCCAAAAACGCGATCCGGAAAAATGCTGCGCCGCACGATCCAGACGAt                                                                                                                               >  2:1252539/1‑140 (MQ=255)  cgcACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCGGCGCACGTCTGGTTTGTCTCGCAATTGCCAAAAACGCGATCCGGAAAAATGCTGCGCCGCACGATCCAGACGAtt                                                                                                                              >  1:1629219/1‑140 (MQ=255)      cTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCGGCGCACGTCTGGTTTGTCTCGCAATTGCCAAAAACGCGATCCGGAAAAATGCTGCGCCGCACGATCCAGACGATTTGCg                                                                                                                          <  1:850665/140‑1 (MQ=255)      cTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCGGCGCACGTCTGGTTTGTCTCGCAATTGCCAAAAACGCGATCCGGAAAAATGCTGCGCCGCACGATCCAGACGATTTGCg                                                                                                                          >  1:93564/1‑140 (MQ=255)      cTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCGGCGCACGTCTGGTTTGTCTCGCAATTGCCAAAAACGCGATCCGGAAAAATGCTGCGCCGCACGATCCAGACGATTTGCg                                                                                                                          <  1:1219478/140‑1 (MQ=255)      cTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCGGCGCACGTCTGGTTTGTCTCGCAATTGCCAAAAACGCGATCCGGAAAAATGCTGCGCCGCACGATCCAGACGATTTGCg                                                                                                                          <  1:378957/140‑1 (MQ=255)      cTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCGGCGCACGTCTGGTTTGTCTCGCAATTGCCAAAAACGCGATCCGGAAAAATGCTGCGCCGCACGATCCAGACGATTTGCg                                                                                                                          <  2:1615414/140‑1 (MQ=255)      cTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCGGCGCACGTCTGGTTTGTCTCGCAATTGCCAAAAACGCGATCCGGAAAAATGCTGCGCCGCACGATCCAGACGATTTGCg                                                                                                                          >  1:1598195/1‑140 (MQ=255)         gCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCGGCGCACGTCTGGTTTGTCTCGCAATTGCCAAAAACGCGATCCGGAAAAATGCTGCGCCGCACGATCCAGACGATTTGCGAAg                                                                                                                       >  1:1178027/1‑140 (MQ=255)             gagaAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCGGCGCACGTCTGGTTTGTCTCGCAATTGCCAAAAACGCGATCCGGAAAAATGCTGCGCCGCACGATCCAGACGATTTGCGAAGGAcg                                                                                                                   <  2:970967/140‑1 (MQ=255)             gagaAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCGGCGCACGTCTGGTTTGTCTCGCAATTGCCAAAAACGCGATCCGGAAAAATGCTGCGCCGCACGATCCAGACGATTTGCGAAGGAcg                                                                                                                   <  1:639502/140‑1 (MQ=255)             gagaAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCGGCGCACGTCTGGTTTGTCTCGCAATTGCCAAAAACGCGATCCGGAAAAATGCTGCGCCGCACGATCCAGACGATTTGCGAAGGAcg                                                                                                                   <  1:1451962/140‑1 (MQ=255)                aaGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCGGCGCACGTCTGGTTTGTCTCGCAATTGCCAAAAACGCGATCCGGAAAAATGCTGCGCCGCACGATCCAGACGATTTGCGAAGGACGCGa                                                                                                                <  2:177023/140‑1 (MQ=255)                aaGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCGGCGCACGTCTGGTTTGTCTCGCAATTGCCAAAAACGCGATCCGGAAAAATGCTGCGCCGCACGATCCAGACGATTTGCGAAGGACGCGa                                                                                                                <  1:406642/140‑1 (MQ=255)                aaGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCGGCGCACGTCTGGTTTGTCTCGCAATTGCCAAAAACGCGATCCGGAAAAATGCTGCGCCACACGATCCAGACGATTTGCGAAGGACGCGa                                                                                                                <  1:956344/140‑1 (MQ=255)                 aGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCGGCGCACGTCTGGTTTGTCTCGCAATTGCCAAAAACGCGATCCGGAAAAATGCTGCGCCGCACGATCCAGACGATTTGCGAAGGACGCGAt                                                                                                               >  1:209953/1‑140 (MQ=255)                  ggCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCGGCGCACGTCTGGTTTGTCTCGCAATTGCCAAAAACGCGATCCGGAAAAATGCTGCGCCGCACGATCCAGACGATTTGCGAAGGACGCGATc                                                                                                              >  2:1509969/1‑140 (MQ=255)                   gCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCGGCGCACGTCTGGTTTGTCTCGCAATTGCCAAAAACGCGATCCGGAAAAATGCTGCGCCGCACGATCCAGACGATTTGCGAAGGACGCGATcc                                                                                                             >  1:552389/1‑140 (MQ=255)                   gCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCGGCGCACGTCTGGTTTGTCTCGCAATTGCCAAAAACGCGATCCGGAAAAATGCTGCGCCGCACGATCCAGACGATTTGCGAAGGACGCGATcc                                                                                                             >  1:960873/1‑140 (MQ=255)                   gCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCGGCGCACGTCTGGTTTGTCTCGCAATTGCCAAAAACGCGATCCGGAAAAATGCTGCGCCGCACGATCCAGACGATTTGCGAAGGACGCGATcc                                                                                                             <  1:511256/140‑1 (MQ=255)                   gCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCGGCGCACGTCTGGTTTGTCTCGCAATTGCCAAAAACGCGATCCGGAAAAATGCTGCGCCGCACGATCCAGACGATTTGCGAAGGACGCGATcc                                                                                                             >  1:456520/1‑140 (MQ=255)                          tGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCGGCGCACGTCTGGTTTGTCTCGCAATTGCCAAAAACGCGATCCGGAAAAATGCTGCGCCGCACGATCCAGACGATTTGCGAAGGACGCGATCCTGGAGAt                                                                                                      >  1:1579665/1‑140 (MQ=255)                          tGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCGGCGCACGTCTGGTTTGTCTCGCAATTGCCAAAAACGCGATCCGGAAAAATGCTGCGCCGCACGATCCAGACGATTTGCGAAGGACGCGATCCTGGAGAt                                                                                                      <  1:166332/140‑1 (MQ=255)                                 ggtggACAGCCAGATTGGCAACTTTGGCCGCCCGGCGCACGTCTGGTTTGTCTCGCAATTGCCAAAAACGCGATCCGGAAAAATGCTGCGCCGCACGATCCAGACGATTTGCGAAGGACGCGATCCTGGAGATCTgacga                                                                                               <  2:506178/140‑1 (MQ=255)                                 ggtggACAGCCAGATTGGCAACTTTGGCCGCCCGGCGCACGTCTGGTTTGTCTCGCAATTGCCAAAAACGCGATCCGGAAAAATGCTGCGCCGCACGATCCAGACGATTTGCGAAGGACGCGATCCTGGAGATCTgacga                                                                                               <  2:1598195/140‑1 (MQ=255)                                 ggtggACAGCCAGATTGGCAACTTTGGCCGCCCGGCGCACGTCTGGTTTGTCTCGCAATTGCCAAAAACGCGATCCGGAAAAATGCTGCGCCGCACGATCCAGACGATTTGCGAAGGACGCGATCCTGGAGATCTgacga                                                                                               >  2:872339/1‑140 (MQ=255)                                 ggtggACAGCCAGATTGGCAACTTTGGCCGCCCGGCGCACGTCTGGTTTGTCTCGCAATTGCCAAAAACGCGATCCGGAAAAATGCTGCGCCGCACGATCCAGACGATTTGCGAAGGACGCGATCCTGGAGATCTgacga                                                                                               <  2:93564/140‑1 (MQ=255)                                  gtggACAGCCAGATTGGCAACTTTGGCCGCCCGGCGCACGTCTGGTTTGTCTCGCAATTGCCAAAAACGCGATCCGGAAAAATGCTGCGCCGCACGATCCAGACGATTTGCGAAGGACGCGATCCTGGAGATCTgacgac                                                                                              <  1:1509969/140‑1 (MQ=255)                                   tggACAGCCAGATTGGCAACTTTGGCCGCCCGGCGCACGTCTGGTTTGTCTCGCAATTGCCAAAAACGCGATCCGGAAAAATGCTGCGCCGCACGATCCAGACGATTTGCGAAGGACGCGATCCTGGAGATCTGACGAcc                                                                                             <  1:144548/140‑1 (MQ=255)                                   tggACAGCCAGATTGGCAACTTTGGCCGCCCGGCGCACGTCTGGTTTGTCTCGCAATTGCCAAAAACGCGATCCGGAAAAATGCTGCGCCGCACGATCCAGACGATTTGCGAAGGACGCGATCCTGGAGATCTGACGAcc                                                                                             <  2:209953/140‑1 (MQ=255)                                   tggACAGCCAGATTGGCAACTTTGGCCGCCCGGCGCACGTCTGGTTTGTCTCGCAATTGCCAAAAACGCGATCCGGAAAAATGCTGCGCCGCACGATCCAGACGATTTGCGAAGGACGCGATCCTGGAGATCTGACGAcc                                                                                             <  2:1628543/140‑1 (MQ=255)                                   tggACAGCCAGATTGGCAACTTTGGCCGCCCGGCGCACGTCTGGTTTGTCTCGCAATTGCCAAAAACGCGATCCGGAAAAATGCTGCGCCGCACGATCCAGACGATTTGCGAAGGACGCGATCCTGGAGATCTGACGAcc                                                                                             <  1:564690/140‑1 (MQ=255)                                     gACAGCCAGATTGGCAACTTTGGCCGCCCGGCGCACGTCTGGTTTGTCTCGCAATTGCCAAAAACGCGATCCGGAAAAATGCTGCGCCGCACGATCCAGACGATTTGCGAAGGACGCGATCCTGGAGATCTGACGACCAt                                                                                           <  2:746652/140‑1 (MQ=255)                                     gACAGCCAGATTGGCAACTTTGGCCGCCCGGCGCACGTCTGGTTTGTCTCGCAATTGCCAAAAACGCGATCCGGAAAAATGCTGCGCCGCACGATCCAGACGATTTGCGAAGGACGCGATCCTGGAGATCTGACGACCAt                                                                                           >  1:95574/1‑140 (MQ=255)                                       cAGCCAGATTGGCAACTTTGGCCGCCCGGCGCACGTCTGGTTTGTCTCGCAATTGCCAAAAACGCGATCCGGAAAAATGCTGCGCCGCACGATCCAGACGATTTGCGAAGGACGCGATCCTGGAGATCTGACGACCATtg                                                                                         <  2:1178027/140‑1 (MQ=255)                                            aGATTGGCAACTTTGGCCGCCCGGCGCACGTCTGGTTTGTCTCGCAATTGCCAAAAACGCGATCCGGAAAAATGCTGCGCCGCACGATCCAGACGATTTGCGAAGGACGCGATCCTGGAGATTTgacgac                                                                                              <  2:1053202/130‑1 (MQ=255)                                            aGATTGGCAACTTTGGCCGCCCGGCGCACGTCTGGTTTGTCTCGCAATTGCCAAAAACGCGATCCGGAAAAATGCTGCGCCGCACGATCCAGACGATTTGCGAAGGACGCGATCCTGGAGATTTgacgac                                                                                              >  1:1053202/1‑130 (MQ=255)                                            aGATTGGCAACTTTGGCCGCCCGGCGCACGTCTGGTTTGTCTCGCAATTGCCAAAAACGCGATCCGGAAAAATGCTGCGCCGCACGATCCAGACGATTTGCGAAGGACGCGATCCTGGAGATCTGACGACCATTgatgat                                                                                    >  1:453389/1‑140 (MQ=255)                                               ttGGCAACTTTGGCCGCCCGGCGCACGTCTGGTTTGTCTCGCAATTGCCAAAAACGCGATCCGGAAAAATGCTGCGCCGCACGATCCAGACGATTTGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCt                                                                                 >  2:567478/1‑140 (MQ=255)                                                 ggCAACTTTGGCCGCCCGGCGCACGTCTGGTTTGTCTCGCAATTGCCAAAAACGCGATCCGGAAAAATGCTGCGCCGCACGATCCAGACGATTTGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGc                                                                               <  1:1252539/140‑1 (MQ=255)                                                    aaCTTTGGCCGCCCGGCGCACGTCTGGTTTGTCTCGCAATTGCCAAAAACGCGATCCGGAAAAATGCTGCGCCGCACGATCCAGACGATTTGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGcgtc                                                                            >  1:294209/1‑140 (MQ=255)                                                    aaCTTTGGCCGCCCGGCGCACGTCTGGTTTGTCTCGCAATTGCCAAAAACGCGATCCGGAAAAATGCTGCGCCGCACGATCCAGACGATTTGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGcgtc                                                                            >  2:866402/1‑140 (MQ=255)                                                     aCTTTGGCCGCCCGGCGCACGTCTGGTTTGTCTCGCAATTGCCAAAAACGCGATCCGGAAAAATGCTGCGCCGCACGATCCAGACGATTTGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATG‑TCCTGcgtcgt                                                                          <  2:960873/140‑1 (MQ=255)                                                      cTTTGGCCGCCCGGCGCACGTCTGGTTTGTCTCGCAATTGCCAAAAACGCGATCCGGAAAAATGCTGCGCCGCACGATCCAGACGATTTGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGcgtcgt                                                                          <  1:866402/140‑1 (MQ=255)                                                        ttGGCCGCCCGGCGCACGTCTGGTTTGTCTCGCAATTGCCAAAAACGCGATCCGGAAAAATGCTGCGCCGCACGATCCAGACGATTTGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTg                                                                        >  1:732339/1‑140 (MQ=255)                                                          ggCCGCCCGGCGCACGTCTGGTTTGTCTCGCAATTGCCAAAAACGCGATCCGGAAAAATGCTGCGCCGCACGATCCAGACGATTTGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGa                                                                      <  1:326051/140‑1 (MQ=255)                                                                 cGGCGCACGTCTGGTTTGTCTCGCAATTGCCAAAAACGCGATCCGGAAAAATGCTGCGCCGCACGATCCAGACGATTTGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATc                                                               >  2:1021181/1‑140 (MQ=255)                                                                    cgcACGTCTGGTTTGTCTCGCAATTGCCAAAAACGCGATCCGGAAAAATGCTGCGCCGCACGATCCAGACGATTTGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGc                                                            <  2:952211/140‑1 (MQ=255)                                                                            tGGTTTGTCTCGCAATTGCCAAAAACGCGATCCGGAAAAATGCTGCGCCGCACGATCCAGACGATTTGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGAt                                                    <  1:363392/140‑1 (MQ=255)                                                                            tGGTTTGTCTCGCAATTGCCAAAAACGCGATCCGGAAAAATGCTGCGCCGCACGATCCAGACGATTTGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGAt                                                    <  1:54680/140‑1 (MQ=255)                                                                             ggTTTGTCTCGCAATTGCCAAAAACGCGATCCGGAAAAATGCTGCGCCGCACGATCCAGACGATTTGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATg                                                   <  1:326619/140‑1 (MQ=255)                                                                             ggTTTGTCTCGCAATTGCCAAAAACGCGATCCGGAAAAATGCTGCGCCGCACGATCCAGACGATTTGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATg                                                   <  2:1629219/140‑1 (MQ=255)                                                                              gTTTGTCTCGCAATTGCCAAAAACGCGATCCGGAAAAATGCTGCGCCGCACGATCCAGACGATTTGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATgg                                                  <  1:1240754/140‑1 (MQ=255)                                                                                   tctcGCAATTGCCAAAAACGCGATCCGGAAAAATGCTGCGCCGCACGATCCAGACGATTTGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAagag                                             >  1:1597209/1‑140 (MQ=255)                                                                                   tctcGCAATTGCCAAAAACGCGATCCGGAAAAATGCTGCGCCGCACGATCCAGACGATTTGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAagag                                             <  1:625011/140‑1 (MQ=255)                                                                                   tctcGCAATTGCCAAAAACGCGATCCGGAAAAATGCTGCGCCGCACGATCCAGACGATTTGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGAAGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAagag                                             >  2:1505761/1‑140 (MQ=255)                                                                                   tctcGCAATTCCCAAAAACGCGATCCGGAAAAATGCTGCGCCGCACGATCCAGACGATTTGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAagag                                             <  2:359842/140‑1 (MQ=255)                                                                                     tcGCAATTGCCAAAAACGCGATCCGGAAAAATGCTGCGCCGCACGATCCAGACGATTTGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTa                                           >  1:797881/1‑140 (MQ=255)                                                                                       gCAATTGCCAAAAACGCGATCCGGAAAAATGCTGCGCCGCACGATCCAGACGATTTGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAgg                                         <  1:980519/140‑1 (MQ=255)                                                                                       gCAATTGCCAAAAACGCGATCCGGAAAAATGCTGCGCCGCACGATCCAGACGATTTGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGAAGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAgg                                         <  1:1505761/140‑1 (MQ=255)                                                                                          aTTGCCAAAAACGCGATCCGGAAAAATGCTGCGCCGCACGATCCAGACGATTTGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTa                                      <  2:456520/140‑1 (MQ=255)                                                                                            tGCCAAAAACGCGATCCGGAAAAATGCTGCGCCGCACGATCCAGACGATTTGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTAtt                                    <  2:95574/140‑1 (MQ=255)                                                                                            tGCCAAAAACGCGATCCGGAAAAATGCTGCGCCGCACGATCCAGACGATTTGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTAtt                                    <  1:1439684/140‑1 (MQ=255)                                                                                                   aaCGCGATCCGGAAAAATGCTGCGCCGCACGATCCAGACGATTTGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGAt                             <  2:453389/140‑1 (MQ=255)                                                                                                              gAAAAATGCTGCGCCGCACGATCCAGACGATTTGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCgg                  >  1:1399191/1‑140 (MQ=255)                                                                                                              gAAAAATGCTGCGCCGCACGATCCAGACGATTTGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCgg                  >  1:755223/1‑140 (MQ=255)                                                                                                               aaaaaTGCTGCGCCGCACGATCCAGACGATTTGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGt                 <  2:1239728/140‑1 (MQ=255)                                                                                                                    tgctgcGCCGCACGATCCAGACGATTTGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGt                 >  1:1601423/1‑135 (MQ=255)                                                                                                                    tgctgcGCCGCACGATCCAGACGATTTGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGt                 <  2:1601423/135‑1 (MQ=255)                                                                                                                      ctgcGCCGCACGATCCAGACGATTTGCGAAGGACGCGATCCTGGAGATCTGACGACCTTTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCg          <  2:1485437/140‑1 (MQ=255)                                                                                                                      ctgcGCCGCACGATCCAGACGATTTGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCg          <  2:755223/140‑1 (MQ=255)                                                                                                                      ctgcGCCGCACGATCCAGACGATTTGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCg          <  2:797881/140‑1 (MQ=255)                                                                                                                      ctgcGCCGCACGATCCAGACGATTTGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCg          <  2:1399191/140‑1 (MQ=255)                                                                                                                       tgcGCCGCACGATCCAGACGATTTGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCgg         <  2:294209/140‑1 (MQ=255)                                                                                                                       tgcGCCGCACGATCCAGACGATTTGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCgg         <  1:1470987/140‑1 (MQ=255)                                                                                                                         cgccgcACGATCCAGACGATTTGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCa       <  2:359203/140‑1 (MQ=255)                                                                                                                         cgccgcACGATCCAGACGATTTGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCa       <  1:1184931/140‑1 (MQ=255)                                                                                                                         cgccgcACGATCCAGACGATTTGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCa       <  1:567478/140‑1 (MQ=255)                                                                                                                         cgccgcACGATCCAGACGATTTGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCa       <  1:1021181/140‑1 (MQ=255)                                                                                                                              cACGATCCAGACGATTTGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACtgtg  >  2:1280354/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                  GCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCGGCGCACGTCTGGTTTGTCTCGCAATTGCCAAAAACGCGATCCGGAAAAATGCTGCGCCGCACGATCCAGGCGATTTGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTG  >  NC_000913/354368‑354632

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑