Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	204,758	T→A	L89Q (CTG→CAG)	rnhB →	RNase HII

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	204,758	0	T	A	100.0%	175.1 / NA	49	L89Q (CTG→CAG)	rnhB	RNase HII
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (21/28);  total (21/28)

AATGATTCCAAAAAGCTGAGCGAAAAACGCCGTCTGGCGCTCTATGAAGAGATCAAAGAGAAAGCGTTGAGCTGGAGTCTGGGCCGCGCGGAACCCCACGAAATCGACGAGCTGAACATTCTTCATGCGACCATGCTGGCGATGCAGCGTGCCGTCGCTGGGCTGCATATTGCGCCGGAATATGTGTTGATTGATGGTAACCGCTGCCCGAAATTACCGATGCCTGCGATGGCTGTGGTGAAAGGCGATAGCCGCGTACCGGAAATCAGTG  >  NC_000913/204622‑204892                                                                                                                                         |                                                                                                                                       aaTGATTCCAAAAAGCTGAGCGAAAAACGCCGTCTGGCGCTCTATGAAGAGATCAAAGAGAAAGCGTTGAGCTGGAGTCTGGGCCGCGCGGAACCCCACGAAATCGACGAGCTGAACATTCTTCATGCGACCATGCAGGc                                                                                                                                     <  2:992727/140‑1 (MQ=255)     aTTCCAAAAAGCTGAGCGAAAAACGCCGTCTGGCGCTCTATGAAGAGATCAAAGAGAAAGCGTTGAGCTGGAGTCTGGGCCGCGCGGAACCCCACGAAATCGACGAGCTGAACATTCTTCATGCGACCATGCAGGCGATg                                                                                                                                 <  2:860570/140‑1 (MQ=255)       tCCAAAAAGCTGAGCGAAAAACGCCGTCTGGCGCTCTATGAAGAGATCAAAGAGAAAGCGTTGAGCTGGAGTCTGGGCCGCGCGGAACCCCACGAAATCGACGAGCTGAACATTCTTCATGCGACCATGCAGGCGATGCa                                                                                                                               >  2:1214949/1‑140 (MQ=255)       tCCAAAAAGCTGAGCGAAAAACGCCGTCTGGCGCTCTATGAAGAGATCAAAGAGAAAGCGTTGAGCTGGAGTCTGGGCCGCGCGGAACCCCACGAAATCGACGAGCTGAACATTCTTCATGCGACCATGCAGGCGATGCa                                                                                                                               >  2:213882/1‑140 (MQ=255)             aaGCTGAGCGAAAAACGCCGTCTGGCGCTCTATGAAGAGATCAAAGAGAAAGCGTTGAGCTGGAGTCTGGGCCGCGCGGAACCCCACGAAATCGACGAGCTGAACATTCTTCATGCGACCATGCAGGCGATGCAGCGTGc                                                                                                                         <  2:408573/140‑1 (MQ=255)             aaGCTGAGCGAAAAACGCCGTCTGGCGCTCTATGAAGAGATCAAAGAGAAAGCGTTGAGCTGGAGTCTGGGCCGCGCGGAACCCCACGAAATCGACGAGCTGAACATTCTTCATGCGACCATGCAGGCGATGCAGCGTGc                                                                                                                         <  1:377561/140‑1 (MQ=255)               gCTGAGCGAAAAACGCCGTCTGGCGCTCTATGAAGAGATCAAAGAGAAAGCGTTGAGCTGGAGTCTGGGCCGCGCGGAACCCCACGAAATCGACGAGCTGAACATTCTTCATGCGACCATGCAGGCGATGCAGCGTGc                                                                                                                         <  2:919511/138‑1 (MQ=255)               gCTGAGCGAAAAACGCCGTCTGGCGCTCTATGAAGAGATCAAAGAGAAAGCGTTGAGCTGGAGTCTGGGCCGCGCGGAACCCCACGAAATCGACGAGCTGAACATTCTTCATGCGACCATGCAGGCGATGCAGCGTGc                                                                                                                         >  1:919511/1‑138 (MQ=255)                  gAGCGAAAAACGCCGTCTGGCGCTCTATGAAGAGATCAAAGAGAAAGCGTTGAGCTGGAGTCTGGGCCGCGCGGAACCCCACGAAATCGACGAGCTGAACATTCTTCATGCGACCATGCAGGCGATGCAGCGTGCCGTCg                                                                                                                    <  1:1273402/140‑1 (MQ=255)                   aGCGAAAAACGCCGTCTGGCGCTCTATGAAGAGATCAAAGAGAAAGCGTTGAGCTGGAGTCTGGGCCGCGCGGAACCCCACGAAATCGACGAGCTGAACATTCTTCATGCGACCATGCAGGCGATGCAGCGTGCCGTCGc                                                                                                                   >  2:161483/1‑140 (MQ=255)                    gCGAAAAACGCCGTCTGGCGCTCTATGAAGAGATCAAAGAGAAAGCGTTGAGCTGGAGTCTGGGCCGCGCGGAACCCCACGAAATCGACGAGCTGAACATTCTTCATGCGACCATGCAGGCGATGCAGCGTGCCGTCGCt                                                                                                                  <  2:1258072/140‑1 (MQ=255)                                    ggCGCTCTATGAAGAGATCAAAGAGAAAGCGTTGAGCTGGAGTCTGGGCCGCGCGGAACCCCACGAAATCGACGAGCTGAACATTCTTCATGCGACCATGCAGGCGATGCAGCGTGCCGTCGCTGGGCTGCATATTgcgc                                                                                                  <  2:803533/140‑1 (MQ=255)                                    ggCGCTCTATGAAGAGATCAAAGAGAAAGCGTTGAGCTGGAGTCTGGGCCGCGCGGAACCCCACGAAATCGACGAGCTGAACATTCTTCATGCGACCATGCAGGCGATGCAGCGTGCCGTCGCTGGGCTGCATATTgcgc                                                                                                  <  2:1085286/140‑1 (MQ=255)                                               aaGAGATCAAAGAGAAAGCGTTGAGCTGGAGTCTGGGCCGCGCGGAACCCCACGAAATCGACGAGCTGAACATTCTTCATGCGACCATGCAGGCGATGCAGCGTGCCGTCGCTGGGCTGCATATTGCGCCGGAATAtgtg                                                                                       <  1:563465/140‑1 (MQ=255)                                                agagATCAAAGAGAAAGCGTTGAGCTGGAGTCTGGGCCGCGCGGAACCCCACGAAATCGACGAGCTGAACATTCTTCATGCGACCATGCAGGCGATGCAGCGTGCCGTCGCTGGGCTGCATATTGCGCCGGAATATgtgt                                                                                      <  1:161483/140‑1 (MQ=255)                                                agagATCAAAGAGAAAGCGTTGAGCTGGAGTCTGGGCCGCGCGGAACCCCACGAAATCGACGAGCTGAACATTCTTCATGCGACCATGCAGGCGATGCAGCGTGCCGTCGCTGGGCTGCATATTGCGCCGGAATATgtgt                                                                                      <  1:277177/140‑1 (MQ=255)                                                   gaTCAAAGAGAAAGCGTTGAGCTGGAGTCTGGGCCGCGCGGAACCCCACGAAATCGACGAGCTGAACATTCTTCATGCGACCATGCAGGCGATGCAGCGTGCCGTCGCTGGGCTGCATATTGCGCCGGAATATGTGttga                                                                                   <  2:198151/140‑1 (MQ=255)                                                   gaTCAAAGAGAAAGCGTTGAGCTGGAGTCTGGGCCGCGCGGAACCCCACGAAATCGACGAGCTGAACATTCTTCATGCGACCATGCAGGCGATGCAGCGTGCCGTCGCTGGGCTGCATATTGCGCCGGAATATGTGttga                                                                                   >  1:1024013/1‑140 (MQ=255)                                                    aTCAAAGAGAAAGCGTTGAGCTGGAGTCTGGGCCGCGCGGAACCCCACGAAATCGACGAGCTGAACATTCTTCATGCGACCATGCAGGCGATGCAGCGTGCCGTCGCTGGGCTGCATATTGCGCCGGAATATGTGttga                                                                                   <  1:1046159/139‑1 (MQ=255)                                                    aTCAAAGAGAAAGCGTTGAGCTGGAGTCTGGGCCGCGCGGAACCCCACGAAATCGACGAGCTGAACATTCTTCATGCGACCATGCAGGCGATGCAGCGTGCCGTCGCTGGGCTGCATATTGCGCCGGAATATGTGttga                                                                                   >  2:1046159/1‑139 (MQ=255)                                                     tCAAAGAGAAAGCGTTGAGCTGGAGTCTGGGCCGCGCGGAACCCCACGAAATCGACGAGCTGAACATTCTTCATGCGACCATGCAGGCGATGCAGCGTGCCGTCGCTGGGCTGCATATTGCGCCGGAATATGTGttgatt                                                                                 <  1:407978/140‑1 (MQ=255)                                                     tCAAAGAGAAAGCGTTGAGCTGGAGTCTGGGCCGCGCGGAACCCCACGAAATCGACGAGCTGAACATTCTTCATGCGACCATGCAGGCGATGCAGCGTGCCGTCGCTGGGCTGCATATTGCGCCGGAATATGTGttgatt                                                                                 >  2:668100/1‑140 (MQ=255)                                                     tCAAAGAGAAAGCGTTGAGCTGGAGTCTGGGCCGCGCGGAACCCCACGAAATCGACGAGCTGAACATTCTTCATGCGACCATGCAGGCGATGCAGCGTGCCGTCGCTGGGCTGCATATTGCGCCGGAATATGTGttgatt                                                                                 <  1:213882/140‑1 (MQ=255)                                                     tCAAAGAGAAAGCGTTGAGCTGGAGTCTGGGCCGCGCGGAACCCCACGAAATCGACGAGCTGAACATTCTTCATGCGACCATGCAGGCGATGCAGCGTGCCGTCGCTGGGCTGCATATTGCGCCGGAATATGTGttgatt                                                                                 >  2:139136/1‑140 (MQ=255)                                                     tCAAAGAGAAAGCGTTGAGCTGGAGTCTGGGCCGCGCGGAACCCCACGAAATCGACGAGCTGAACATTCTTCATGCGACCATGCAGGCGATGCAGCGTGCCGTCGCTGGGCTGCATATTGCGCCGGAATATGTGttgatt                                                                                 >  2:1219661/1‑140 (MQ=255)                                                      aaaaGAGAAAGCGTTGAGCTGGAGTCTGGGCCGCGCGGAACCCCACGAAATCGACGACCTGAACATTCTTCATGCGACAATGCAGGCGATGCAGCGTGCCGTCGCTGGGCTGCATATTGCGCCGGAATATGTGttgattg                                                                                <  2:474665/139‑1 (MQ=255)                                                       aaaGAGAAAGCGTTGAGCTGGAGTCTGGGCCGCGCGGAACCCCACGAAATCGACGAGCTGAACATTCTTCATGCGACCATGCAGGCGATGCAGCGTGCCGTCGCTGGGCTGCATATTGCGCCGGAATATGTGttgattga                                                                               >  1:455/1‑140 (MQ=255)                                                            gaAAGCGTTGAGCTGGAGTCTGGGCCGCGCGGAACCCCACGAAATCGACGAGCTGAACATTCTTCATGCGACCATGCAGGCGATGCAGCGTGCCGTCGCTGGGCTGCATATTGCGCCGGAATATGTGTTGATTGATGGTa                                                                          >  2:807917/1‑140 (MQ=255)                                                              aaGCGTTGAGCTGGAGTCTGGGCCGCGCGGAACCCCACGAAATCGACGAGCTGAACATTCTTCATGCGACCATGCAGGCGATGCAGCGTGCCGTCGCTGGGCTGCATATTGCGCCGGAATATGTGTTGATTGATGGTAAc                                                                        >  2:533573/1‑140 (MQ=255)                                                                     gAGCTGGAGTCTGGGCCGCGCGGAACCCCACGAAATCGACGAGCTGAACATTCTTCATGCGACCATGCAGGCGATGCAGCGTGCCGTCGCTGGGCTGCATATTGCGCCGGAATATGTGTTGATTGATGGTAACCg                                                                      <  2:1069094/135‑1 (MQ=255)                                                                     gAGCTGGAGTCTGGGCCGCGCGGAACCCCACGAAATCGACGAGCTGAACATTCTTCATGCGACCATGCAGGCGATGCAGCGTGCCGTCGCTGGGCTGCATATTGCGCCGGAATATGTGTTGATTGATGGTAACCg                                                                      >  1:1069094/1‑135 (MQ=255)                                                                                tGGGCCGCGCGGAACCCCACGAAATCGACGAGCTGAACATTCTTCATGCGACCATGCAGGCGATGCAGCGTGCCGTCGCTGGGCTGCATATTGCGCCGGAATATGTGTTGATTGATGGTAACCGCTGCCCGAAATTAc                                                        <  2:969604/138‑1 (MQ=255)                                                                                tGGGCCGCGCGGAACCCCACGAAATCGACGAGCTGAACATTCTTCATGCGACCATGCAGGCGATGCAGCGTGCCGTCGCTGGGCTGCATATTGCGCCGGAATATGTGTTGATTGATGGTAACCGCTGCCCGAAATTAc                                                        >  1:969604/1‑138 (MQ=255)                                                                                tGGGCCGCGCGGAACCCCACGAAATCGACGAGCTGAACATTCTTCATGCGACCATGCAGGCGATGCAGCGTGCCGTCGCTGGGCTGCATATTGCGCCGGAATATGTGTTGATTGATGGTAACCGCTGCCCGAAATTACCg                                                      <  1:668100/140‑1 (MQ=255)                                                                                  ggCCGCGCGGAACCCCACGAAATCGACGAGCTGAACATTCTTCATGCGACCATGCAGGCGATGCAGCGTGCCGTCGCTGGGCTGCATATTGCGCCGGAATATGTGTTGATTGATGGTAACCGCTGCCCGAAATTACCGAt                                                    <  2:455/140‑1 (MQ=255)                                                                                     cgcgcgGAACCCCACGAAATCGACGAGCTGAACATTCTTCATGCGACCATGCAGGCGATGCAGCGTGCCGTCGCTGGGCTGCATATTGCGCCGGAATATGTGTTGATTGATGGTAACCGCTGCCCGAAATTACCGATGcc                                                 >  1:142805/1‑140 (MQ=255)                                                                                     cgcgcgGAACCCCACGAAATCGACGAGCTGAACATTCTTCATGCGACCATGCAGGCGATGCAGCGTGCCGTCGCTGGGCTGCATATTGCGCCGGAATATGTGTTGATTGATGGTAACCGCTGCCCGAAATTACCGATGcc                                                 <  1:1061286/140‑1 (MQ=255)                                                                                     cgcgcgGAACCCCACGAAATCGACGAGCTGAACATTCTTCATGCGACCATGCAGGCGATGCAGCGTGCCGTCGCTGGGCTGCATATTGCGCCGGAATATGTGTTGATTGATGGTAACCGCTGCCCGAAATTACCGATGc                                                  >  1:338734/1‑139 (MQ=255)                                                                                     cgcgcgGAACCCCACGAAATCGACGAGCTGAACATTCTTCATGCGACCATGCAGGCGATGCAGCGTGCCGTCGCTGGGCTGCATATTGCGCCGGAATATGTGTTGATTGATGGTAACCGCTGCCCGAAATTACCGATGc                                                  <  2:338734/139‑1 (MQ=255)                                                                                       cgcgGAACCCCACGAAATCGACGAGCTGAACATTCTTCATGCGACCATGCAGGCGATGCAGCGTGCCGTCGCTGGGCTGCATATTGCGCCGGAATATGTGTTGATTGATGGTAACCGCTGCCCGAAATTACCGATGCCTg                                               >  1:931576/1‑140 (MQ=255)                                                                                                        tCGACGAGCTGAACATTCTTCATGCGACCATGCAGGCGATGCAGCGTGCCGTCGCTGGGCTGCATATTGCGCCGGAATATGTGTTGATTGATGGTAACCGCTGCCCGAAATTACCGATGCCTGCGATGGCTGTGGTGaaa                              >  1:984347/1‑140 (MQ=255)                                                                                                                cTGAACATTCTTCATGCGACCATGCAGGCGATGCAGCGTGCCGTCGCTGGGCTGCATATTGCGCCGGAATATGTGTTGATTGATGGTAACCGCTGCCCGAAATTACCGATGCCTGCGATGGCTGTGGTGAAAGGCGATAg                      >  2:273286/1‑140 (MQ=255)                                                                                                                     cATTCTTCATGCGACCATGCAGGCGATGCAGCGTGCCGTCGCTGGGCTGCATATTGCGCCGGAATATGTGTTGATTGATGGTAACCGCTGCCCGAAATTACCGATGCCTGCGATGGCTGTGGTGAAAGGCGATAGCcgcg                 <  1:273286/140‑1 (MQ=255)                                                                                                                     cATTCTTCATGCGACCATGCAGGCGATGCAGCGTGCCGTCGCTGGGCTGCATATTGCGCCGGAATATGTGTTGATTGATGGTAACCGCTGCCCGAAATTACCGATGCCTGCGATGGCTGTGGTGAAAGGCGATAGCcgcg                 <  2:1338133/140‑1 (MQ=255)                                                                                                                     cATTCTTCATGCGACCATGCAGGCGATGCAGCGTGCCGTCGCTGGGCTGCATATTGCGCCGGAATATGTGTTGATTGATGGTAACCGCTGCCCGAAATTACCGATGCCTGCGATGGCTGTGGTGAAAGGCGATAGCcgcg                 <  1:807917/140‑1 (MQ=255)                                                                                                                        tcttcATGCGACCATGCAGGCGATGCAGCGTGCCGTCGCTGGGCTGCATATTGCGCCGGAATATGTGTTGATTGATGGTAACCGCTGCCCGAAATTACCGATGCCTGCGATGGCTGTGGTGAAAGGCGATAGCCGCGTAc              >  1:22734/1‑140 (MQ=255)                                                                                                                        tcttcATGCGACCATGCAGGCGATGCAGCGTGCCGTCGCTGGGCTGCATATTGCGCCGGAATATGTGTTGATTGATGGTAACCGCTGCCCGAAATTACCGATGCCTGCGATGGCTGTGGTGAAAGGCGATAGCCGCGTAc              <  2:931576/140‑1 (MQ=255)                                                                                                                                 gACCATGCAGGCGATGCAGCGTGCCGTCGCTGGGCTGCATATTGCGCCGGAATATGTGTTGATTGATGGTAACCGCTGCCCGAAATTACCGATGCCTGCGATGGCTGTGGTGAAAGGCGATAGCCGCGTACCGGAAATCa     <  2:488237/140‑1 (MQ=255)                                                                                                                                  aCCATGCAGGCGATGCAGCGTGCCGTCGCTGGGCTGCATATTGCGCCGGAATATGTGTTGATTGATGGTAACCGCTGCCCGAAATTACCGATGCCTGCGATGGCTGTGGTGAAAGGCGATAGCCGCGTACCGGAAATCAg    >  2:7176/1‑140 (MQ=255)                                                                                                                                    cATGCAGGCGATGCAGCGTGCCGTCGCTGGGCTGCATATTGCGCCGGAATATGTGTTGATTGATGGTAACCGCTGCCCGAAATTACCGATGCCTGCGATGGCTGTGGTGAAAGGCGATAGCCGCGTACCGGAAATCAGTg  <  2:984347/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                       AATGATTCCAAAAAGCTGAGCGAAAAACGCCGTCTGGCGCTCTATGAAGAGATCAAAGAGAAAGCGTTGAGCTGGAGTCTGGGCCGCGCGGAACCCCACGAAATCGACGAGCTGAACATTCTTCATGCGACCATGCTGGCGATGCAGCGTGCCGTCGCTGGGCTGCATATTGCGCCGGAATATGTGTTGATTGATGGTAACCGCTGCCCGAAATTACCGATGCCTGCGATGGCTGTGGTGAAAGGCGATAGCCGCGTACCGGAAATCAGTG  >  NC_000913/204622‑204892

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑