Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,339,065 G→C A119G (GCA→GGA)  gyrA ← DNA gyrase subunit A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,339,0650GC94.1% 119.7 / ‑0.3 34A119G (GCA→GGA) gyrADNA gyrase subunit A
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base C (28/4);  total (29/5)
Fisher's exact test for biased strand distribution p-value = 2.76e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

TAGGAATTTTGGTTGGCATGACGTCCGGAATTTTTTCCGTGCCGTCATAGTTATCAACGAAATCGACCGTCTCTTTTTCGAGATCGGCCATCAGTTCATGGGCAATTTTCGCCAGACGGATTTCCGTATAACGCATTGCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGCCGAGTCACCATGGGGATGGTATTTA  >  NC_000913/2338928‑2339196
                                                                                                                                         |                                                                                                                                   
tAGGAATTTTGGTTGGCATGACGTCCGGAATTTTTTCCGTGCCGTCATAGTTATCAACGAAATCGACCGTCTCTTTTTCGAGATCGGCCATCAGTTCATGGGCAATTTTCGCCAGACGGATTTCCGTATAACGCATTccc                                                                                                                                   >  2:1021642/1‑140 (MQ=255)
  ggAATTTTGGTTGGCATGACGTCCGGAATTTTTTCCGTGCCGTCATAGTTATCAACGAAATCGACCGTCTCTTTTTCGAGATCGGCCATCAGTTCATGGGCAATTTTCGCCAGACGGATTTCCGTATAACGCATTcccgc                                                                                                                                 >  2:1791753/1‑140 (MQ=255)
      ttttGGTTGGCATGACGTCCGGAATTTTTTCCGTGCCGTCATAGTTATCAACGAAATCGACCGTCTCTTTTTCGAGATCGGCCATCAGTTCATGGGCAATTTTCGCCAGACGGATTTCCGTATAACGCATTCCCGCCGCa                                                                                                                             >  1:1345103/1‑140 (MQ=255)
        ttggttggCATGACGTCCGGAATTTTTTCCGTGCCGTCATAGTTATCAACGAAATCGACCGTCTCTTTTTCGAGATCGGCCATCAGTTCATGGGCAATTTTCGCCAGCCGGATTTCCGTATAACGCATTCCCGCCGCaga                                                                                                                           >  1:2022159/1‑140 (MQ=255)
             tggCATGACGTCCGGAATTTTTTCCGTGCCGTCATAGTTATCAACGAAATCGACCGTCTCTTTTTCGAGATCGGCCATCAGTTCATGGGCAATTTTCGCCAGACGGATTTCCGTATAACGCATTCCCGCCGCAGAGTCGc                                                                                                                      >  1:1400145/1‑140 (MQ=255)
                         cGGAATTTTTTCCGTGCCGTCATAGTTATCAACGAAATCGACCGTCTCTTTTTCGAGATCGGCCATCAGTTCATGGGCAATTTTCGCCAGACGGATTTCCGTATAACGCATTCCCGCCGCAGAGTCGCCGTCGATAGAAc                                                                                                          >  1:2643977/1‑140 (MQ=255)
                         cGGAATTTTTTCCGTGCCGTCATAGTTATCAACGAAATCGACCGTCTCTTTTTCGAGATCGGCCATCAGTTCATGGGCAATTTTCGCCAGACGGATTTCCGTATAACGCATTCCCGCCGCAGAGTCGCCGTCGATAGAAc                                                                                                          >  2:1674462/1‑140 (MQ=255)
                              ttttttCCGTGCCGTCATAGTTATCAACGAAATCGACCGTCTCTTTTTCGAGATCGGCCATCAGTTCATGGGCAATTTTCGCCAGACGGATTTCCGTATAACGCATTCCCGCCGCAGAGTCGCCGTCGATAGAACCGAAg                                                                                                     >  1:2248167/1‑140 (MQ=255)
                                      gTGCCGTCATAGTTATCAACGAAATCGACCGTCTCTTTTTCGAGATCGGCCATCAGTTCATGGGCAATTTTCGCCAGACGGATTTCCGTATAACGCATTCCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTg                                                                                             >  1:2645857/1‑140 (MQ=255)
                                       ggCCGTCATATTTCTCAACGAAACCGCCCGCCTGTTTGTGGAGATGGGCCATGGCTTCGGGGGAATTTCGCGCCGGGCGGATTTCCGTATACCGCATACCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGa                                                                                            <  2:2243662/139‑1 (MQ=255)
                                       ggCCGTCATAGTTATCAACGAAATCGCCCGCCTTTTTTTCGAGATCGGCCATCAGTTCAGGGGCATTTTCCGCCAGACGGATTTCCGTATAACGCATCCCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGa                                                                                            <  2:1639147/139‑1 (MQ=255)
                                       ggCCGTCATAGTTATCAACGAAACCGCCCGTCTTTTTTTCGAGATCGGCCATCATTTCTGGGGCATTTTCCCCCAGACGGATTTCCTTATAACGCATCCCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGa                                                                                            <  2:1114041/139‑1 (MQ=255)
                                            tCATAGTTATCAACGAAATCGACCGTCTCTTTTTCGAGATCGGCCATCAGTTCATGGGCAATTTTCGCCAGACGGATTTCCGTATAACGCATTCCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTc                                                                                       >  2:1324102/1‑140 (MQ=255)
                                               tatttATCAACGAAACCGCCCTTCTTTTTGTCGGGAGCGGCCATCATTTCAGGGAAAATTTCCGCAAGACGGATTCCCGTAAAACGCTTTCCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTAc                                                                                    <  2:1477881/137‑1 (MQ=255)
                                                    aTCAACGAAATCGACCGTCTCTTTTTCGAGATCGGCCATCAGTTCATGGGCAATTTTCGCCAGACGGATTTCCGTATAACGCATTCCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCa                                                                               >  1:2182234/1‑140 (MQ=255)
                                                    aTCAACGAAATCGACCGTCTCTTTTTCGAGATCGGCCATCAGTTCATGGGCAATTTTCGCCAGACGGATTTCCGTATAACGCATTCCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCa                                                                               >  2:2425965/1‑140 (MQ=255)
                                                     tCAACGAAATCGACCGTCTCTTTTTCGAGATCGGCCATCAGTTCATGGGCAATTTTCGCCAGACGGATTTCCGTATAACGCATTCCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCat                                                                              >  1:1148143/1‑140 (MQ=255)
                                                      cAACGAAATCGACCGTCTCTTTTTCGAGATCGGCCATCAGTTCATGGGCAATTTTCGCCAGACGGATTTCCGTATAACGCATTCCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCata                                                                             >  1:1065078/1‑140 (MQ=255)
                                                      cAACGAAATCGACCGTCTCTTTTTCGAGATCGGCCATCAGTTCATGGGCAATTTTCGCCAGACGGATTTCCGTATAACGCATTCCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCata                                                                             >  2:1700229/1‑140 (MQ=255)
                                                       aaCGAAATCGACCGTCTCTTTTTCGAGATCGGCCATCAGTTCATGGGCAATTTTCGCCAGACGGATTTCCGTATAACGCATTCCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCatat                                                                            >  1:2617918/1‑140 (MQ=255)
                                                       aaCGAAATCGACCGTCTCTTTTTCGAGATCGGCCATCAGTTCATGGGCAATTTTCGCCAGACGGATTTCCGTATAACGCATTCCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCatat                                                                            >  2:1993777/1‑140 (MQ=255)
                                                            aaTCGACCGTCTCTTTTTCGAGATCGGCCATCAGTTCATGGGCAATTTTCGCCAGACGGATTTCCGTATAACGCATTGCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGc                                                                       >  2:779631/1‑140 (MQ=255)
                                                            aaTCGACCGTCTCTTTTTCGAGATCGGCCATCAGTTCATGGGCAATTTTCGCCAGACGGATTTCCGTATAACGCATTCCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGc                                                                       >  1:1444005/1‑140 (MQ=255)
                                                            aaTCGACCGTCTCTTTTTCGAGATCGGCCATCAGTTCATGGGCAATTTTCGCCAGACGGATTTCCGTATAACGCATTCCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGc                                                                       >  1:2332343/1‑140 (MQ=255)
                                                             accGACCGCCTTTTTTTCGAGATCGGCCATCAGTTCAGGGGCAATTTCCGCCAGCCGGTTTTCCGTATAACGCATTCCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCa                                                                      <  2:2282265/138‑1 (MQ=255)
                                                                      ctctTTTTCGAGATCGGCCATCAGTTCATGGGCAATTTTCGCCAGACGGATTTCCGTATAACGCATTCCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATg                                                             >  1:2016117/1‑140 (MQ=255)
                                                                            ttCGAGATCGGCAATCAGTTCAGGGGCATTTTTCGCCAGACGGTTTTCCGTATAACCCTTTCCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTgcg                                                       <  2:1956803/140‑1 (MQ=255)
                                                                              cGAGTTCGCCCATCAGTTCAGGGTTACTTTCCGCAGGCGGGATTCCCTAATACCCCTTTCCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGcc                                                     <  2:2106376/140‑1 (MQ=255)
                                                                              cGAGACCGGCCATCATTTCAGGGGCAATTTCCGCCAGACGGTTTTCCGTATAACGCATCCCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGcc                                                     <  2:1400145/140‑1 (MQ=255)
                                                                                     ggCCATCAGTTCATGGGCATTTTCCGCCAGACGGTTTTCCGTATAACGCTTTCCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGa                                              <  1:1702615/140‑1 (MQ=255)
                                                                                     gcccaccaGTTGATGGGCAATTTCCGCCGGGCGTTTTCCGGTATAACGCATCCCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGa                                              <  2:2028119/138‑1 (MQ=255)
                                                                                      gCCATCAGTTCATGGGCAATTTTCGCCAGACGGATTTCCGTATAACGCATTGCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGAc                                             <  1:779631/140‑1 (MQ=255)
                                                                                          tCAGTTCATGGAAATTTTCCGCCGGACGGTTTTCCGTATAACGCATTCCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATc                                         <  1:2382942/140‑1 (MQ=255)
                                                                                          tCAGTTCAGGGGCATTTTCCGCCAGACGGTTTCCCGTATACCGCATTCCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATc                                         <  1:2544127/140‑1 (MQ=255)
                                                                                          tCAGTTCAGGGGAATTTTCCGCCAGACGGATTCCCGTATAACGCTTTCCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATc                                         <  1:2048668/140‑1 (MQ=255)
                                                                                          ccatttCAGGGGCATTTTCCCCCAGAGGGTTTCCCGTATACCGCATTCCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATc                                         <  2:1305300/136‑1 (MQ=255)
                                                                                           cAGTTCATGGGCAATTTTCGCCAGACGGATTTCCGTATAACGCATTCCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCg                                        >  1:2324622/1‑140 (MQ=255)
                                                                                             tttCAGGGGAATTTTCCGCCAGCCGGTTTTCCGTATAACGCTTTCCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCgtg                                      <  1:1674462/139‑1 (MQ=255)
                                                                                              ttCATGGGCAATTTTCGCCAGACGGATTTCCGTATAACGCATTCCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCgtgt                                     >  2:1215043/1‑140 (MQ=255)
                                                                                                cATGGGCAATTTTCGCCAGACGGATTTCCGTATAACGCATTCCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCa                                   >  1:1081081/1‑140 (MQ=255)
                                                                                                cATGGGCAATTTTCGCCAGACGGATTTCCGTATAACGCATTCCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCa                                   >  2:2557069/1‑140 (MQ=255)
                                                                                                 aTGGGCAATTTTCGCCAGACGGATTTCCGTATAACGCATTCCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCAt                                  >  1:1608217/1‑140 (MQ=255)
                                                                                                 aTGGGCAATTTTCGCCAGACGGATTTCCGTATAACGCATTCCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCAt                                  >  1:1810593/1‑140 (MQ=255)
                                                                                                  tGGGCAATTTTCGCCAGACGGATTTCCGTATAACGCATTCCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGACTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATa                                 >  2:1390468/1‑140 (MQ=255)
                                                                                                      cAATTTTCGCCAGACGGATTTCCGTATAACGCATTCCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGAcc                             >  2:1835469/1‑140 (MQ=255)
                                                                                                            tCGCCAGACGGATTTCCGTATAACGCATTCCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGCCGAg                       <  2:2182234/140‑1 (MQ=255)
                                                                                                             ccccAGAGGGTTTCCCGTATAACGCTTCCCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGCCGAGt                      <  1:2532509/138‑1 (MQ=255)
                                                                                                               ccAGACGGATTTCCGTATAACGCATTCCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGCCGAGTCa                    >  2:1702591/1‑140 (MQ=255)
                                                                                                                 agccGGATTCCCGTATACCGCACTCCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGCCGAGTCAcc                  <  2:2248167/137‑1 (MQ=255)
                                                                                                                 aGACGTATTCCTGTATACCGCTTTCCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGCCGAGTCAcc                  <  1:1021642/140‑1 (MQ=255)
                                                                                                                   gggtaTTCCCTTATACCGCTTTCCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGCCGAGTCACCAt                <  1:2149515/136‑1 (MQ=255)
                                                                                                                       ttttCGGTATAACGCTTCCCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCAGACGATCGTGTCATAGACCGCCGAGTCACCATgggg            <  2:1345103/139‑1 (MQ=255)
                                                                                                                       tttcccGTAAAACGCTTCCCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGCCGAGTCACCATgggg            <  2:2645857/136‑1 (MQ=255)
                                                                                                                                 aaCGCATTCCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGCCGAGTCACCATGGGGATGGTATTTa  >  1:2227327/1‑140 (MQ=255)
                                                                                                                                         |                                                                                                                                   
TAGGAATTTTGGTTGGCATGACGTCCGGAATTTTTTCCGTGCCGTCATAGTTATCAACGAAATCGACCGTCTCTTTTTCGAGATCGGCCATCAGTTCATGGGCAATTTTCGCCAGACGGATTTCCGTATAACGCATTGCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGCCGAGTCACCATGGGGATGGTATTTA  >  NC_000913/2338928‑2339196

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑