Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,244,885	T→A	intergenic (‑144/‑339)	uxaC ← / → exuT	D‑glucoronate/D‑galacturonate isomerase/hexuronate transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,244,885	0	T	A	100.0%	258.3 / NA	76	intergenic (‑144/‑339)	uxaC/exuT	D‑glucoronate/D‑galacturonate isomerase/hexuronate transporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (35/41);  total (35/41)

CTCTCAACGAGTGAGCTAGCTTGCTTATGGTGCGATGCTGACAAAGTTATCACACCAATTTCCAGAGTCCGAAGATATTTTCGTGAGTTAGATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGT  >  NC_000913/3244752‑3245019                                                                                                                                      |                                                                                                                                       ctctCAACGAGTGAGCTAGCTTGCTTATGGTGCGATGCTGACAAAGTTATCACACCAATTTCCAGAGTCCGAAGATATTTTCGTGAGTTAGATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAAACTCGCt                                                                                                                                  >  2:1807753/1‑140 (MQ=255)  tctcAACGAGTGAGCTAGCTTGCTTATGGTGCGATGCTGACAAAGTTATCACACCAATTTCCAGAGTCCGAAGATATTTTCGTGAGTTAGATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAAACTCGCTg                                                                                                                                 <  2:2634765/140‑1 (MQ=255)       aCGAGTGAGCTAGCTTGCTTATGGTGCGATGCTGACAAAGTTATCACACCAATTTCCAGAGTCCGAAGATATTTTCGTGAGTTAGATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAAACTCGCTGCAGGc                                                                                                                            <  1:1807407/140‑1 (MQ=255)       aCGAGTGAGCTAGCTTGCTTATGGTGCGATGCTGACAAAGTTATCACACCAATTTCCAGAGTCCGAAGATATTTTCGTGAGTTAGATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAAACTCGCTGCAGGc                                                                                                                            <  2:1206017/140‑1 (MQ=255)         gAGTGAGCTAGCTTGCTTATGGTGCGATGCTGACAAAGTTATCACACCAATTTCCAGAGTCCGAAGATATTTTCGTGAGTTAGATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAAACTCGCTGCAGGCcg                                                                                                                          <  2:2564498/140‑1 (MQ=255)           gTGAGCTAGCTTGCTTATGGTGCGATGCTGACAAAGTTATCACACCAATTTCCAGAGTCCGAAGATATTTTCGTGAGTTAGATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAAACTCGCTGCAGGCcgcg                                                                                                                        >  2:2561191/1‑140 (MQ=255)           gTGAGCTAGCTTGCTTATGGTGCGATGCTGACAAAGTTATCACACCAATTTCCAGAGTCCGAAGATATTTTCGTGAGTTAGATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAAACTCGCTGCAGGCcgcg                                                                                                                        >  2:2409208/1‑140 (MQ=255)            tGAGCTAGCTTGCTTATGGTGCGATGCTGACAAAGTTATCACACCAATTTCCAGAGTCCGAAGATATTTTCGTGAGTTAGATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAAACTCGCTGCAGGCCgcgc                                                                                                                       <  1:1128660/140‑1 (MQ=255)            tGAGCTAGCTTGCTTATGGTGCGATGCTGACAAAGTTATCACACCAATTTCCAGAGTCCGAAGATATTTTCGTGAGTTAGATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAAACTCGCTGCAGGCCgcgc                                                                                                                       <  2:1840123/140‑1 (MQ=255)                ctagctTGCTTATGGTGCGATGCTGACAAAGTTATCACACCAATTTCCAGAGTCCGAAGATATTTTCGTGAGTTAGATCAATAAACGTAGTTAAAAAAATTGCTCTCAAAGTGGTAAAACTCGCTGCAGGCCGCGCCAGt                                                                                                                   <  1:1029881/140‑1 (MQ=255)                  agctTGCTTATGGTGCGATGCTGACAAAGTTATCACACCAATTTCCAGAGTCCGAAGATATTTTCGTGAGTTAGATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAAACTCGCTGCAGGCCGCGCCAGTAc                                                                                                                 >  1:1703988/1‑140 (MQ=255)                    cttgcttATGGTGCGATGCTGACAAAGTTATCACACCAATTTCCAGAGTCCGAAGATATTTTCGTGAGTTAGATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAAACTCGCTGCAGGCCGCGCCAGTACTg                                                                                                               >  1:1417912/1‑140 (MQ=255)                       gcttATGGTGCGATGCTGACAAAGTTATCACACCAATTTCCAGAGTCCGAAGATATTTTCGTGAGTTAGATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAAACTCGCTGCAGGCCGCGCCAGTACTGGcc                                                                                                            <  1:1435461/140‑1 (MQ=255)                        cttATGGTGCGATGCTGACAAAGTTATCACACCAATTTCCAGAGTCCGAAGATATTTTCGTGAGTTAGATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAAACTCGCTGCAGGCCGCGCCAGTACTGGCCt                                                                                                           >  1:2047154/1‑140 (MQ=255)                        cttATGGTGCGATGCTGACAAAGTTATCACACCAATTTCCAGAGTCCGAAGATATTTTCGTGAGTTAGATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAAACTCGCTGCAGGCCGCGCCAGTACTGGCCt                                                                                                           >  1:1653015/1‑140 (MQ=255)                          tATGGTGCGATGCTGACAAAGTTATCACACCAATTTCCAGAGTCCGAAGATATTTTCGTGAGTTAGATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAAACTCGCTGCAGGCCGCGCCAGTACTGGCCTTg                                                                                                         >  1:1475690/1‑140 (MQ=255)                             ggTGCGATGCTGACAAAGTTATCACACCAATTTCCAGAGTCCGAAGATATTTTCGTGAGTTAGATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAAACTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTg                                                                                                      <  1:1100293/140‑1 (MQ=255)                             ggTGCGATGCTGACAAAGTTATCACACCAATTTCCAGAGTCCGAAGATATTTTCGTGAGTTAGATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAAACTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTg                                                                                                      >  1:1700601/1‑140 (MQ=255)                                 cGATGCTTACAAAGTTATCACTGCAATTTCTAGTATCCGAGCTTATGTTCGTGGGTTAGATCAATAACCGTAGATAAATAAATTTCTCTCAAACTGGTAAACGTCTCTTCAGGCCGCGGCAGTACTGGCCTTGCTgtcgt                                                                                                  <  2:2631168/140‑1 (MQ=255)                                 cGATGCTGACAAAGTTATCACACCAATTTCCAGAGTCCGAAGATATTTTCGTGAGTTAGATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAAACTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTgtcgt                                                                                                  <  1:1044216/140‑1 (MQ=255)                                  gATGCTGACAAAGTTATCACACCAATTTCCAGAGTCCGAAGATATTTTCGTGAGTTAGATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAAACTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTgtcgtc                                                                                                 <  2:2071770/140‑1 (MQ=255)                                   aTGCTGACAAAGTTATCACACCAATTTCCAGAGTCCGAAGATATTTTCGTGAGTTAGATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAAACTCGCTGCAGGCCGCGCCAGTa                                                                                                                  >  1:1353732/1‑122 (MQ=255)                                   aTGCTGACAAAGTTATCACACCAATTTCCAGAGTCCGAAGATATTTTCGTGAGTTAGATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAAACTCGCTGCAGGCCGCGCCAGTa                                                                                                                  <  2:1353732/122‑1 (MQ=255)                                   aTGCTGACAAAGTTATCACACCAATTTCCAGAGTCCGAAGATATTTTCGTGAGTTAGATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAAACTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCa                                                                                                >  1:1557814/1‑140 (MQ=255)                                   aTGCTGACAAAGTTATCACACCAATTTCCAGAGTCCGAAGATATTTTCGTGAGTTAGATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAAACTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCa                                                                                                >  1:2162437/1‑140 (MQ=255)                                      cTGACAAAGTTATCACACCAATTTCCAGAGTCCGAAGATATTTTCGTGAGTTAGATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAAACTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGt                                                                                             <  1:2229142/140‑1 (MQ=255)                                        gACAAAGTTATCACACCAATTTCCAGAGTCCGAAGATATTTTCGTGAGTTAGATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAAACTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGTaa                                                                                           <  2:2449481/140‑1 (MQ=255)                                         aCAAAGTTATCACACCAATTTCCAGAGTCCGAAGATATTTTCGTGAGTTAGATCAATAAACGTAGTTAAAAGAATTACTCTCAAAGTGGTAAAACTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGTAAt                                                                                          <  1:1655939/140‑1 (MQ=255)                                           aaaGTTATCACACCAATTTCCAGAGTCCGAAGATATTTTCGTGAGTTAGATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAAACTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGTAATGt                                                                                        <  2:1838812/140‑1 (MQ=255)                                           aaaGTTATCACACCAATTTCCAGAGTCCGAAGATATTTTCGTGAGTTAGATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAAACTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGTAATGt                                                                                        <  2:2520633/140‑1 (MQ=255)                                                tATCACACCAATTTCCAGAGTCCGAAGATATTTTCGTGAGTTAGATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAAACTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGTAATGTCCCTa                                                                                   >  2:2602762/1‑140 (MQ=255)                                                tATCACACCAATTTCCAGAGTCCGAAGATATTTTCGTGAGTTAGATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAAACTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGTAATGTCCCTa                                                                                   >  2:2083021/1‑140 (MQ=255)                                                tATCACACCAATTTCCAGAGTCCGAAGATATTTTCGTGAGTTAGATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAAACTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGTAATGTCCCTa                                                                                   >  1:2494632/1‑140 (MQ=255)                                                   cacaCCAATTTCCAGAGTCCGAAGATATTTTCGTGAGTTAGATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAAACTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGTAATGTCCCTACaa                                                                                <  2:2047154/140‑1 (MQ=255)                                                   cacaCCAATTTCCAGAGTCCGAAGATATTTTCGTGAGTTAGATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAAACTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGTAATGTCCCTACaa                                                                                <  1:2118036/140‑1 (MQ=255)                                                    acacCAATTTCCAGAGTCCGAAGATATTTTCGTGAGTTAGATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAAACTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGTAATGTCCCTACaaa                                                                               <  2:2415058/140‑1 (MQ=255)                                                     cacCAATTTCCAGAGTCCGAAGATATTTTCGTGAGTTAGATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAAACTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGTAATGTCCCTACAAat                                                                              >  1:2235607/1‑140 (MQ=255)                                                       ccAATTTCCAGAGTCCGAAGATATTTTCGTGAGTTAGATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAAACTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGTAATGTCCCTACAAatat                                                                            >  2:1078492/1‑140 (MQ=255)                                                         aaTTTCCAGAGTCCGAAGATATTTTCGTGAGTTAGATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAAACTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGTAATGTCCCTACAAATATTc                                                                          <  1:2426378/140‑1 (MQ=255)                                                          aTTTCCAGAGTCCGAAGATATTTTCGTGAGTTAGATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAAACTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGTAATGTCCCTACAAATATTcc                                                                         <  2:1288769/140‑1 (MQ=255)                                                          aTTTCCAGAGTCCGAAGATATTTTCGTGAGTTAGATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAAACTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGTAATGTCCCTACAAATATTcc                                                                         <  2:1417912/140‑1 (MQ=255)                                                           tttCCAGAGTCCGAAGATATTTTCGTGAGTTAGATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAAACTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGTAATGTCCCTACAAATATTccc                                                                        >  1:1151161/1‑140 (MQ=255)                                                                     ccGAAGATATTTTCGTGAGTTAGATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAAACTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGTAATGTCCCTACAAATATTCCCACATTTGTGa                                                              <  1:2409208/140‑1 (MQ=255)                                                                     ccGAAGATATTTTCGTGAGTTAGATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAAACTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGTAATGTCCCTACAAATATTCCCACATTTGTGa                                                              <  1:1620512/140‑1 (MQ=255)                                                                      cGAAGATATTTTCGTGAGTTAGATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAAACTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGTAATGTCCCTACAAATATTCCCACATTTGTGAt                                                             <  2:2235607/140‑1 (MQ=255)                                                                      cGAAGATATTTTCGTGAGTTAGATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAAACTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGTAATGTCCCTACAAATATTCCCACATTTGTGAt                                                             >  1:2070568/1‑140 (MQ=255)                                                                      cGAAGATATTTTCGTGAGTTAGATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAAACTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGTAATGTCCCTACAAATATTCCCACATTTGTGAt                                                             <  2:1700601/140‑1 (MQ=255)                                                                            tatTTTCGTGAGTTAGATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAAACTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGctct                                                       >  1:1780463/1‑140 (MQ=255)                                                                              ttttCGTGAGTTAGATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAAACTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCa                                                     <  2:2197783/140‑1 (MQ=255)                                                                              ttttCGTGAGTTAGATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAAACTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCa                                                     <  1:1741155/140‑1 (MQ=255)                                                                              ttttCGTGAGTTAGATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAAACTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCa                                                     >  1:1228883/1‑140 (MQ=255)                                                                                    tGAGTTAGATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAAACTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCtttt                                               >  2:1469149/1‑140 (MQ=255)                                                                                     gAGTTAGATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAAACTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTa                                              >  2:1860673/1‑140 (MQ=255)                                                                                         tAGATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAAACTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGt                                          <  2:2162437/140‑1 (MQ=255)                                                                                              cAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAAACTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTAt                                     <  2:1817813/140‑1 (MQ=255)                                                                                              cAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAAACTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTAt                                     <  1:1406509/140‑1 (MQ=255)                                                                                              cAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAAACTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTAt                                     <  2:1851750/140‑1 (MQ=255)                                                                                              cAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAAACTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTAt                                     <  1:2561191/140‑1 (MQ=255)                                                                                              cAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAAACTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGTAATGTCCCTACAAATATTCACACATTTGTGATGGCTCTCACCTTTTAAAGTTGTAt                                     <  1:2602762/140‑1 (MQ=255)                                                                                               aaTAAACGTAGTTAAAAAAATTGCTCTCAAAGTGGTAAAACTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCTCCTTTTAAAGTTGTATg                                    <  2:2401840/140‑1 (MQ=255)                                                                                                aTAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAAACTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGa                                   >  2:1398225/1‑140 (MQ=255)                                                                                                aTAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAAACTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGa                                   >  1:2417022/1‑140 (MQ=255)                                                                                                aTAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAAACTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGa                                   >  2:1217535/1‑140 (MQ=255)                                                                                                 tAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAAACTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGAc                                  <  2:1557814/140‑1 (MQ=255)                                                                                                  aaaCGTAGTTAAAAAAATTACTCTCAAAGTGGTAAAACTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACa                                 >  1:1572205/1‑140 (MQ=255)                                                                                                  aaaCGTAGTTAAAAAAATTACTCTCAAAGTGGTAAAACTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACa                                 <  1:2083021/140‑1 (MQ=255)                                                                                                  aaaCGTAGTTAAAAAAATTACTCTCAAAGTGGTAAAACTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACa                                 >  2:1429874/1‑140 (MQ=255)                                                                                                     cGTAGTTAAAAAAATTACTCTCAAAGTGGTAAAACTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGt                              <  1:1258938/140‑1 (MQ=255)                                                                                                      gTAGTTAAAAAAATTACTCTCAAAGTGGTAAAACTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGtt                             >  2:2101609/1‑140 (MQ=255)                                                                                                      gTAGTTAAAAAAATTACTCTCAAAGTGGTAAAACTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGtt                             >  2:2626003/1‑140 (MQ=255)                                                                                                       tAGTTAAAAAAATTACTCTCAAAGTGGTAAAACTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTa                            >  2:2239283/1‑140 (MQ=255)                                                                                                           tAAAAAAATTACTCTCAAAGTGGTAAAACTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCtt                        >  1:2160567/1‑140 (MQ=255)                                                                                                                  aTTACTCTCAAAGTGGTAAAACTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCg                 <  2:2494632/140‑1 (MQ=255)                                                                                                                            aaGTGGTAAAACTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCa       <  2:2120060/140‑1 (MQ=255)                                                                                                                                 gTAAAACTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGt  >  1:2599666/1‑140 (MQ=255)                                                                                                                                 gTAAAACTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGt  >  1:1845272/1‑140 (MQ=255)                                                                                                                                 gTAAAACTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGt  >  1:1201826/1‑140 (MQ=255)                                                                                                                                      |                                                                                                                                       CTCTCAACGAGTGAGCTAGCTTGCTTATGGTGCGATGCTGACAAAGTTATCACACCAATTTCCAGAGTCCGAAGATATTTTCGTGAGTTAGATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGT  >  NC_000913/3244752‑3245019

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑