Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,209,8270TG37.9% 24.7 / 14.5 29noncoding (54/76 nt)gltVtRNA‑Glu
Reads supporting (aligned to +/- strand):  ref base T (8/10);  new base G (11/0);  total (19/10)
Fisher's exact test for biased strand distribution p-value = 3.49e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.25e-01
Rejected as consensus: Frequency below/above cutoff threshold.

ACCTTAAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTC‑GAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGT  >  NC_000913/4209688‑4209872
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accttaAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGt                                                <  1:1046472/140‑1 (MQ=32)
    taAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTC‑Ga                                           >  2:997573/1‑140 (MQ=32)
    taAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTC‑Ga                                           >  2:327187/1‑140 (MQ=33)
     aaaGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTC‑Gaa                                          <  2:712858/140‑1 (MQ=32)
       agaagCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTC‑GAATc                                        >  2:390419/1‑140 (MQ=33)
       agaagCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGGTC‑GAATc                                        >  2:1237861/1‑140 (MQ=18)
          agCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTC‑GAATcccc                                     >  1:451944/1‑140 (MQ=33)
           gCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCTGATTcccc                                     >  2:914433/1‑140 (MQ=16)
           gCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGGTC‑GAATccccc                                    >  2:463801/1‑139 (MQ=18)
            cGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGGTC‑GCAACCCCTa                                   >  2:1245174/1‑140 (MQ=12)
            cGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGGTC‑GAATCCCCTa                                   >  1:1560813/1‑140 (MQ=18)
            cGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGGTC‑GAATCCCCTa                                   >  2:1193802/1‑140 (MQ=18)
             gTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTC‑GAATCCCCTAg                                  <  2:1201120/140‑1 (MQ=32)
              ttCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGGTC‑GAATCCCCTAgg                                 >  1:29336/1‑140 (MQ=18)
              ttCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGCCGGAAAAAGGGGATC‑GGATTCCCTAgg                                 >  1:1438545/1‑140 (MQ=11)
               tCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGGTC‑GAATCCCCTAggg                                >  2:1354374/1‑140 (MQ=18)
                cTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTC‑GAATCCCCTAgggg                               <  1:997573/140‑1 (MQ=32)
                   tGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGGTC‑GAATCCCCTAGGGGACg                            >  1:913184/1‑140 (MQ=18)
                    gCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGGTC‑GAATCCCCTAGGGGACGc                           >  1:1530336/1‑140 (MQ=18)
                     cAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTC‑GAATCCCCTAGGGGACGcc                          <  1:544316/140‑1 (MQ=32)
                          cTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTC‑GAATCCCCTAGGGGACGCCACTTg                     >  2:1392852/1‑140 (MQ=32)
                           tCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTC‑GAATCCCCTAGGGGACGCCACTTGc                    >  1:92877/1‑140 (MQ=32)
                           tCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGGTC‑GAATCCCCTAGGGGACGCCACTTGc                    >  2:123424/1‑140 (MQ=18)
                             acacaGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTC‑GAATCCCCTAGGGGGCGCCACTTGCTg                  >  1:801044/1‑140 (MQ=17)
                                    ttGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTC‑GAATCCCCTAGGGGACGCCACTTGCTGGTTtgtg           <  1:814579/140‑1 (MQ=32)
                                      gTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTC‑GAATCCCCTAGGGGACGCCACTTGCTGGTTTgtgag         <  1:1392852/140‑1 (MQ=32)
                                      gTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTC‑GAATCCCCTAGGGGACGCCACTTGCTGGTTTgtgag         <  2:370775/140‑1 (MQ=32)
                                         tGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTC‑GAATCCCCTAGGGGACGCCACTTGCTGGTTTgtgagtga      <  1:567477/140‑1 (MQ=32)
                                         tGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGGTC‑GAATCCCCTAGGGGACGCCACTTGCTGGTTTgtgagtga      >  2:1313837/1‑140 (MQ=18)
                                            tAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTC‑GAATCCCCTAGGGGACGCCACTTGCTGGTTtgt            <  2:987657/131‑1 (MQ=32)
                                            tAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTC‑GAATCCCCTAGGGGACGCCACTTGCTGGTTtgt            >  1:987657/1‑131 (MQ=33)
                                             aGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAAGGGGTC‑GAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGGGAAAgg  >  2:567477/1‑139 (MQ=12)
                                               aaaGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTC‑GAATCCCCTAGGGGACGCCACTTGCTGGTTTgtgag         >  1:451333/1‑131 (MQ=11)
                                               aaaGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTC‑GAATCCCCTAGGGGACGCCACTTGCTGGTTTgtgag         <  2:451333/131‑1 (MQ=11)
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ACCTTAAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTC‑GAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGT  >  NC_000913/4209688‑4209872

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑