Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	15,344	T→G	intergenic (+46/‑101)	dnaJ → / → insL‑1	chaperone protein DnaJ/IS186/IS421 transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	15,344	0	T	G	100.0%	205.5 / NA	52	intergenic (+46/‑101)	dnaJ/insL‑1	chaperone protein DnaJ/IS186/IS421 transposase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (17/35);  total (17/35)

CTTCGGTGGCCCAACCGGCGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTG  >  NC_000913/15208‑15480                                                                                                                                         |                                                                                                                                         cTTCGGTGGCCCAACCGGCGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGGGCg                                                                                                                                       <  2:1359757/140‑1 (MQ=255)   tCGGTGGCCCAACCGGCGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGGGCGtt                                                                                                                                     <  2:1577126/140‑1 (MQ=255)   tCGGTGGCCCAACCGGCGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGGGCGtt                                                                                                                                     >  1:169305/1‑140 (MQ=255)   tCGGTGGCCCAACCGGCGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGAAGGCCTGGGTAAAAATAGGGGGCGtt                                                                                                                                     >  1:1349326/1‑140 (MQ=255)      gTGGCCCAACCGGCGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGGGCGTTGaa                                                                                                                                  >  1:51407/1‑140 (MQ=255)           ccAACCGGCGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGGGCGtt                                                                                                                                     <  1:633460/132‑1 (MQ=255)           ccAACCGGCGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGGGCGtt                                                                                                                                     >  2:633460/1‑132 (MQ=255)              aCCGGCGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGGGCGTTGAAGATATGCg                                                                                                                          <  1:1312823/140‑1 (MQ=255)                cGGCGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGGGCGTTGAAGATATGCGAg                                                                                                                        <  1:128156/140‑1 (MQ=255)                cGGCGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGGGCGTTGAAGATATGCGAg                                                                                                                        <  2:1473336/140‑1 (MQ=255)                cGGCGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGGGCGTTGAAGATATGCGAg                                                                                                                        <  2:1441368/140‑1 (MQ=255)                cGGCGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGGGCGTTGAAGATATGCGAg                                                                                                                        <  2:970087/140‑1 (MQ=255)                cGGCGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGGGCGTTGAAGATATGCGAg                                                                                                                        <  2:1488041/140‑1 (MQ=255)                   cGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGGGCGTTGAAGATATGCGAGCAc                                                                                                                     >  2:897826/1‑140 (MQ=255)                   cGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGGGCGTTGAAGATATGCGAGCAc                                                                                                                     <  1:747251/140‑1 (MQ=255)                                  gcgcTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTAGGTAAAAATAGGGGGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCggg                                                                                                      <  1:1405752/140‑1 (MQ=255)                                    gcTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGGGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCggggg                                                                                                    >  2:204292/1‑139 (MQ=255)                                    gcTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGGGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGa                                                                                                    >  2:1265235/1‑140 (MQ=255)                                    gcTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGGGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGa                                                                                                    >  2:517725/1‑140 (MQ=255)                                    gcTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGACCGTGGGCAGGCCTGGTTAAAAATAGGGGGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGa                                                                                                    <  2:300951/140‑1 (MQ=255)                                      tCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGGGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATc                                                                                                  <  2:869627/140‑1 (MQ=255)                                       cAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGGGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCa                                                                                                 >  1:1001078/1‑140 (MQ=255)                                             gCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGGGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCa                                                                                           <  2:258700/140‑1 (MQ=255)                                              cttcttTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGGGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCAt                                                                                          <  1:1047197/140‑1 (MQ=255)                                              cttcttTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGGGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCAt                                                                                          <  1:82497/140‑1 (MQ=255)                                              cttcttTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGGGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCAt                                                                                          <  1:653414/140‑1 (MQ=255)                                              cttcttTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGAAGGCCTGGGTAAAAATAGGGGGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCAt                                                                                          <  2:1349326/140‑1 (MQ=255)                                                  tttGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGGGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGGGGACTcccccaaac                                                                                      >  2:1047197/1‑134 (MQ=255)                                                    tGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGGGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAgcgc                                                                                    <  2:1199714/140‑1 (MQ=255)                                                     gATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGGGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCt                                                                                   <  2:36490/140‑1 (MQ=255)                                                      aTGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGGGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGGTCACTCCCATAAacgcca                                                                                  >  1:938568/1‑138 (MQ=255)                                                          tgtgAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGGGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACtt                                                                              <  1:1395159/140‑1 (MQ=255)                                                           gtgAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGGGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTa                                                                             <  1:1255373/140‑1 (MQ=255)                                                           gtgAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGGGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTa                                                                             >  1:1295533/1‑140 (MQ=255)                                                           gtgAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGGGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTa                                                                             <  1:786640/140‑1 (MQ=255)                                                            tgAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGGGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGGGCACTCCCCTAAAAGCTAACTTaa                                                                            >  2:459786/1‑140 (MQ=255)                                                            tgAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGGGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTaa                                                                            <  1:1477449/140‑1 (MQ=255)                                                               agaagTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGGGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAggg                                                                         <  1:459786/140‑1 (MQ=255)                                                                  agTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGGGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTtg                                                                      <  1:1124297/140‑1 (MQ=255)                                                                   gTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGGGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGGGGACTCCCCTAAGCGCTAAATTAAGGGgtgt                                                                     >  2:30567/1‑140 (MQ=255)                                                                    ttttttGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGGGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTtgtg                                                                    <  1:1265235/140‑1 (MQ=255)                                                                                               ccccAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGGGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGGGCACCCCCCTAAAAGCTAAATTTAGGGGTGTGGGGTTTTGCCTGGTATTATTTTAAGTg                                         >  2:1302247/1‑140 (MQ=255)                                                                                                 ccAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGGGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGcc                                       <  2:1494722/140‑1 (MQ=255)                                                                                                       gCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGGGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGGGCACTCCCCCAAACGCCAACCTAAAGGGTGTGGGGTTTCGCCTGGTATGATTTAAAGTGCCGATGaa                                 >  2:1372377/1‑140 (MQ=255)                                                                                                            cccGTGGGCAGGCCTGGGTAAAAATAGGGGGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTAct                            <  1:30567/140‑1 (MQ=255)                                                                                                            cccGTGGGCAGGCCTGGGTAAAAATAGGGGGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTAct                            <  2:355325/140‑1 (MQ=255)                                                                                                            cccGTGGGCAGGCCTGGGTAAAAATAGGGGGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTAct                            <  2:1295533/140‑1 (MQ=255)                                                                                                                      ggCCTGGGTAAAAATAGGGGGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGGGCACTCCCCTAAACGGTAAATTTAAGGGTGGGGTAGTTAGCCTGCTATGAGTTTAATTGGCGGGGAATTAATTTCACCATaa                  >  2:1475898/1‑140 (MQ=255)                                                                                                                              tAAAAATAGGGGGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAg          <  1:1475898/140‑1 (MQ=255)                                                                                                                              tAAAAATAGGGGGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAg          <  2:1115615/140‑1 (MQ=255)                                                                                                                                   aTAGGGGGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAAtt     <  1:919712/140‑1 (MQ=255)                                                                                                                                   aTAGGGGGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAAtt     <  2:1001078/140‑1 (MQ=255)                                                                                                                                      ggggGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTg  <  1:204292/140‑1 (MQ=255)                                                                                                                                      ggggGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTg  <  1:517725/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                         CTTCGGTGGCCCAACCGGCGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTG  >  NC_000913/15208‑15480

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑