Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,129,834	T→C	K349E (AAA→GAA)	wcaD ←	putative colanic acid polymerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,129,834	0	T	C	100.0%	258.3 / NA	65	K349E (AAA→GAA)	wcaD	putative colanic acid polymerase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (33/32);  total (33/32)

GACAGACGATTAAAAATGCATATTCCGGGCTAAATATCGAACCGGTAAAAAACAGCGATACCGGAGTAAAAAGATATAATTGCACGCGAAAATTGCGGTTATCACCAAAAGCGTTGATCATCATTTTTATCACTTTCCCCATGTACCACAGTGATAAAAACACCGCGAACCAGGAAAAATAAATAATCAGCAGATACAAACCATTGTCTATGGTTTTTCCGACATCCGCACCGTTAAATATTCCGAATGATGCGACATATTCATAAAGTGAG  >  NC_000913/2129699‑2129970                                                                                                                                        |                                                                                                                                         gACAGACGATTAAAAATGCATATTCCGGGCTAAATATCGAACCGGTAAAAAACAGCGATACCGGAGTAAAAAGATATAATTGCACGCGAAAATTGCGGTTATCACCAAAAGCGTTGATCATCATTTTTATCACTTCcccc                                                                                                                                      <  1:882675/140‑1 (MQ=255)     gACGATTAAAAATGCATATTCCGGGCTAAATATCGAACCGGTAAAAAACAGCGATACCGGAGTAAAAAGATATAATTGCACGCGAAAATTGCGGTTATCACCAAAAGCGTTGATCATCATTTTTATCACTTCCCCCATGt                                                                                                                                  >  2:80744/1‑140 (MQ=255)     gACGATTAAAAATGCATATTCCGGGCTAAATATCGAACCGGTAAAAAACAGCGATACCGGAGTAAAAAGATATAATTGCACGCGAAAATTGCGGTTATCACCAAAAGCGTTGATCATCATTTTTATCACTTCCCCCATGt                                                                                                                                  >  1:1393439/1‑140 (MQ=255)       cGATTAAAAATGCATATTCCGGGCTAAATATCGAACCGGTAAAAAACAGCGATACCGGAGTAAAAAGATATAATTGCACGCGAAAATTGCGGTTATCACCAAAAGCGTTGATCATCATTTTTATCACTTCCCCCATGTAc                                                                                                                                <  2:533377/140‑1 (MQ=255)         aTTAAAAATGCATATTCCGGGCTAAATATCGAACCGGTAAAAAACAGCGATACCGGAGTAAAAAGATATAATTGCACGCGAAAATTGCGGTTATCACCAAAAGCGTTGATCATCATTTTTATCACTTCCCCCATGTACca                                                                                                                              <  1:550233/140‑1 (MQ=255)         aTTAAAAATGCATATTCCGGGCTAAATATCGAACCGGTAAAAAACAGCGATACCGGAGTAAAAAGATATAATTGCACGCGAAAATTGCGGTTATCACCAAAAGCGTTGATCATCATTTTTATCACTTCCCCCATGTACca                                                                                                                              <  1:743239/140‑1 (MQ=255)                        tCCGGGCTAAATATCGAACCGGTAAAAAACAGCGATACCGGAGTAAAAAGATATAATTGCACGCGAAAATTGCGGTTATCACCAAAAGCGTTGATCATCATTTTTATCACTTCCCCCATGTACCACAGTGATAAAAacac                                                                                                               >  2:141581/1‑140 (MQ=255)                          cGGGCTAAATATCGAACCGGTAAAAAACAGCGATACCGGAGTAAAAAGATATAATTGCACGCGAAAATTGCGGTTATCACCAAAAGCGTTGATCATCATTTTTATCACTTCCCCCATGTACCACAGTGATAAAAACACcg                                                                                                             <  2:237064/140‑1 (MQ=255)                           gggCTAAATATCGAACCGGTAAAAAACAGCGATACCGGAGTAAAAAGATATAATTGCACGCGAAAATTGCGGTTATCACCAAAAGCGTTGATCATCATTTTTATCACTTCCCCCATGTACCACAGTGATAAAAACACcgc                                                                                                            >  1:657029/1‑140 (MQ=255)                            ggCTAAATATCGAACCGGTAAAAAACAGCGATACCGGAGTAAAAAGATATAATTGCACGCGAAAATTGCGGTTATCACCAAAAGCGTTGATCATCATTTTTATCACTTCCCCCATGTACCACAGTGATAAAAACACcgcg                                                                                                           >  2:73600/1‑140 (MQ=255)                            ggCTAAATATCGAACCGGTAAAAAACAGCGATACCGGAGTAAAAAGATATAATTGCACGCGAAAATTGCGGTTATCACCAAAAGCGTTGATCATCATTTTTATCACTTCCCCCATGTACCACAGTGATAAAAACACcgcg                                                                                                           >  2:959927/1‑140 (MQ=255)                            ggCTAAATATCGAACCGGTAAAAAACAGCGATACCGGAGTAAAAAGATATAATTGCACGCGAAAATTGCGGTTATCACCAAAAGCGTTGATCATCATTTTTATCACTTCCCCCATGTAACACAGTGATAAAAACACcgcg                                                                                                           >  1:732880/1‑140 (MQ=255)                             gCTAAATATCGAACCGGTAAAAAACAGCGATACCGGAGTAAAAAGATATAATTGCACGCGAAAATTGCGGATATCACCAAAAGCGTTGATCATCATTTTTATCACTTCCCCCATGTACCACAGTGATAAAAACACCGCGa                                                                                                          >  2:1257792/1‑140 (MQ=255)                                aaaTATCGAACCGGTAAAAAACAGCGATACCGGAGTAAAAAGATATAATTGCACGCGAAAATTGCGGTTATCACCAAAAGCGTTGATCATCATTTTTATCACTTCCCCCATGTACCACAGTGATAAAAACACCGCGAAcc                                                                                                       >  1:1158029/1‑140 (MQ=255)                                 aaTATCGAACCGGTAAAAAACAGCGATACCGGAGTAAAAAGATATAATTGCACGCGAAAATTGCGGTTATCACCAAAAGCGTTGATCATCATTTTTATCACTTCCCCCATGTACCACAGTGATAAAAACACCGCGAACCa                                                                                                      >  2:588508/1‑140 (MQ=255)                                  atatCGAACCGGTAAAAAACAGCGATACCGGAGTAAAAAGATATAATTGCACGCGAAAATTGCGGTTATCACCAAAAGCGTTGATCATCATTTTTATCACTTCCCCCATGTACCACAGTGATAAAAACACCGCGAACCAg                                                                                                     <  1:1123919/140‑1 (MQ=255)                                    atCGAACCGGTAAAAAACAGCGATACCGGAGTAAAAAGATATAATTGCACGCGAAAATTGCGGTTATCACCAAAAGCGTTGATCATCATTTTTATCACTTCCCCCATGTACCACAGTGATAAAAACACCGCGAACCAGGa                                                                                                   <  1:80744/140‑1 (MQ=255)                                      cGAACCGGTAAAAAACAGCGATACCGGAGTAAAAAGATATAATTGCACGCGAAAATTGCGGTTATCACCAAAAGCGTTGATCATCATTTTTATCACTTCCCCCATGTACCACAGTGATAAAAACACCGCGAACCAGGaaa                                                                                                 <  1:1371998/140‑1 (MQ=255)                                        aaCCGGTAAAAAACAGCGATACCGGAGTAAAAAGATATAATTGCACGCGAAAATTGCGGTTATCACCAAAAGCGTTGATCATCATTTTTATCACTTCCCCCATGTACCACAGTGATAAAAACACCGCGAACCAGGaaaaa                                                                                               >  1:951956/1‑140 (MQ=255)                                        aaCCGGTAAAAAACAGCGATACCGGAGTAAAAAGATATAATTGCACGCGAAAATTGCGGTTATCACCAAAAGCGTTGATCATCATTTTTATCACTTCCCCCATGTACCACAGTGATAAAAACACCGCGAACCAGGa                                                                                                   >  2:1098945/1‑136 (MQ=255)                                        aaCCGGTAAAAAACAGCGATACCGGAGTAAAAAGATATAATTGCACGCGAAAATTGCGGTTATCACCAAAAGCGTTGATCATCATTTTTATCACTTCCCCCATGTACCACAGTGATAAAAACACCGCGAACCAGGa                                                                                                   <  1:1098945/136‑1 (MQ=255)                                           cGGTAAAAAACAGCGATACCGGAGTAAAAAGATATAATTGCACGCGAAAATTGCGGTTATCACCAAAAGCGTTGATCATCATTTTTATCACTTCCCCCATGTACCACAGTGATAAAAACACCGCGAACCAGGAAaaataa                                                                                            >  1:1485153/1‑140 (MQ=255)                                             gTAAAAAACAGCGATACCGGAGTAAAAAGATATAATTGCACGCGAAAATTGCGGTTATCACCAAAAGCGTTGATCATCATTTTTATCACTTCCCCCATGTACCACAGTGATAAAAACACCGCGAACCAGGAAaaataaat                                                                                          >  1:1228813/1‑140 (MQ=255)                                                    aCAGCGATACCGGAGTAAAAAGATATAATTGCACGCGAAAATTGCGGTTATCACCAAAAGCGTTGATCATCATTTTTATCACTTCCCCCATGTACCACAGTGATAAAAACACCGCGAACCAGGAAAAATAAATAATcagc                                                                                   <  2:10960/140‑1 (MQ=255)                                                    aCAGCGATACCGGAGTAAAAAGATATAATTGCACGCGAAAATTGCGGTTATCACCAAAAGCGTTGATCATCATTTTTATCACTTCCCCCATGTACCACAGTGATAAAAACACCGCGAACCAGGAAAAATAAATAATcagc                                                                                   <  2:1573832/140‑1 (MQ=255)                                                      aGCGATACCGGAGTAAAAAGATATAATTGCACGCGAAAATTGCGGTTATCACCAAAAGCGTTGATCATCATTTTTATCACTTCCCCCATGTAACACAGTGATAAAAACACCGCGAACCAGGAAAAATAAATAATcagcag                                                                                 <  2:732880/140‑1 (MQ=255)                                                       gCGATACCGGAGTAAAAAGATATAATTGCACGCGAAAATTGCGGTTATCACCAAAAGCGTTGATCATCATTTTTATCACTTCCCCCATGTACCACAGTGATAAAAACACCGCGAACCAGGAAAAATAAATAATCAGCAGa                                                                                >  2:493144/1‑140 (MQ=255)                                                        cGATACCGGAGTAAAAAGATATAATTGCACGCGAAAATTGCGGTTATCACCAAAAGCGTTGATCATCATTTTTATCACTTCCCCCATGTACCACAGTGATAAAAACACCGCGAACCAGGAAAAATAAATAATCAGCAGAt                                                                               >  1:381610/1‑140 (MQ=255)                                                           tACCGGAGTAAAAAGATATAATTGCACGCGAAAATTGCGGTTATCACCAAAAGCGTTGATCATCATTTTTATCACTTCCCCCATGTACCACAGTGATAAAAACACCGCGAACCAGGAAAAATAAATAATCAGCAGATACa                                                                            <  1:73600/140‑1 (MQ=255)                                                             ccGGAGTAAAAAGATATAATTGCACGCGAAAATTGCGGTTATCACCAAAAGCGTTGATCATCATTTTTATCACTTCCCCCATGTACCACAGTGATAAAAACACCGCGAACCAGGAAAAATAAATAATCAGCAGATACaaa                                                                          >  2:803632/1‑140 (MQ=255)                                                                gAGTAAAAAGATATAATTGCACGCGAAAATTGCGGTTATCACCAAAAGCGTTGATCATCATTTTTATCACTTCCCCCATGTACCACAGTGATAAAAACACCGCGAACCAGGAAAAATAAATAATCAGCAGATACAAACCa                                                                       <  2:1158029/140‑1 (MQ=255)                                                                gAGTAAAAAGATATAATTGCACGCGAAAATTGCGGTTATCACCAAAAGCGTTGATCATCATTTTTATCACTTCCCCCATGTACCACAGTGATAAAAACACCGCGAACCAGGAAAAATAAATAATCAGCAGATACAAACCa                                                                       <  1:131181/140‑1 (MQ=255)                                                                 aGTAAAAAGATATAATTGCACGCGAAAATTGCGGTTATCACCAAAAGCGTTGATCATCATTTTTATCACTTCCCCCATGTACCACAGTGATAAAAACACCGCGAACCAGGAAAAATAAATAATCAGCAGATACAAACCAt                                                                      <  1:959927/140‑1 (MQ=255)                                                                     aaaaGATATAATTGCACGCGAAAATTGCGGTTATCACCAAAAGCGTTGATCATCATTTTTATCACTTCCCCCATGTACCACAGTGATAAAAACACCGCGAACCAGGAAAAATAAATAATCAGCAGATACAAACCATTGTc                                                                  <  2:897754/140‑1 (MQ=255)                                                                          atatAATTGCACGCGAAAATTGCGGTTATCACCAAAAGCGTTGATCATCATTTTTATCACTTCCCCCATGTACCACAGTGATAAAAACACCGCGAACCAGGAAAAATAAATAATCAGCAGATACAAACCATTGTCTATgg                                                             >  2:1173444/1‑140 (MQ=255)                                                                             taATTGCACGCGAAAATTGCGGTTATCACCAAAAGCGTTGATCATCATTTTTATCACTTCCCCCATGTACCACAGTGATAAAAACACCGCGAACCAGGAAAAATAAATAATCAGCAGATACAAACCATTGTCTATGGttt                                                          >  1:617358/1‑140 (MQ=255)                                                                                 tGCACGCGAAAATTGCGGTTATCACCAAAAGCGTTGATCATCATTTTTATCACTTCCCCCATGTACCACAGTGATAAAAACACCGCGAACCAGGAAAAATAAATAATCAGCAGATACAAACCATTGTCTATGGTTTTTcc                                                      <  2:657029/140‑1 (MQ=255)                                                                                     cgcgAAAATTGCGGTTATCACCAAAAGCGTTGATCATCATTTTTATCACTTCCCCCATGTACCACAGTGATAAAAACACCGCGAACCAGGAAAAATAAATAATCAGCAGATACAAACCATTGTCTATGGTTTTTCCGACa                                                  >  2:286025/1‑140 (MQ=255)                                                                                     cgcgAAAATTGCGGTTATCACCAAAAGCGTTGATCATCATTTTTATCACTTCCCCCATGTACCACAGTGATAAAAACACCGCGAACCAGGAAAAATAAATAATCAGCAGATACAAACCATTGTCTATGGTTTTTCCGACa                                                  >  2:1149354/1‑140 (MQ=255)                                                                                           aaTTGCGGTTATCACCAAAAGCGTTGATCATCATTTTTATCACTTCCCCCATGTACCACAGTGATAAAAACACCGCGAACCAGGAAAAATAAATAATCAGCAGATACAAACCATTGTCTATGGTTTTTCCGACATCCGCa                                            <  2:1228813/140‑1 (MQ=255)                                                                                                    tATCACCAAAAGCGTTGATCATCATTTTTATCACTTCCCCCATGTACCACAGTGATAAAAACACCGCGAACCAGGAAAAATAAATAATCAGCAGATACAAACCATTGTCTATGGTTTTTCCGACATCCGCACCGTTAAat                                   <  1:141581/140‑1 (MQ=255)                                                                                                     aTCACCAAAAGCGTTGATCATCATTTTTATCACTTCCCCCATGTACCACAGTGATAAAAACACCGCGAACCAGGAAAAATAAATAATCAGCAGATACAAACCATTGTCTATGGTTTTTCCGACATCCGCACCGTTAAata                                  >  2:1361504/1‑140 (MQ=255)                                                                                                     aTCACCAAAAGCGTTGATCATCATTTTTATCACTTCCCCCATGTACCACAGTGATAAAAACACCGCGAACCAGGAAAAATAAATAATCAGCAGATACAAACCATTGTCTATGGTTTTTCCGACATCCGCACCGTTAAata                                  >  1:844015/1‑140 (MQ=255)                                                                                                            aaaGCGTTGATCATCATTTTTATCACTTCCCCCATGTACCACAGTGATAAAAACACCGCGAACCAGGAAAAATAAATAATCAGCAGATACAAACCATTGTCTATGGTTTTTCCGACATCCGCACCGTTAAATATTCCGaa                           >  1:15010/1‑140 (MQ=255)                                                                                                             aaGCGTTGATCATCATTTTTATCACTTCCCCCATGTACCACAGTGATAAAAACACCGCGAACCAGGAAAAATAAATAATCAGCAGATACAAACCATTGTCTATGGTTTTTCCGACATCCGCACCGTTAAATATTCCGAat                          >  1:1284253/1‑140 (MQ=255)                                                                                                             aaGCGTTGATCATCATTTTTATCACTTCCCCCATGTACCACAGTGATAAAAACACCGCGAACCAGGAAAAATAAATAATCAGCAGATACAAACCATTGTCTATGGTTTTTCCGACATCCGCACCGTTAAATATTCCGAat                          >  1:392872/1‑140 (MQ=255)                                                                                                              aGCGTTGATCATCATTTTTATCACTTCCCCCATGTACCACAGTGATAAAAACACCGCGAACCAGGAAAAATAAATAATCAGCAGATACAAACCATTGTCTATGGTTTTTCCGACATCCGCACCGTTAAATATTCCGAatg                         <  2:1472363/140‑1 (MQ=255)                                                                                                                cGTTGATCATCATTTTTATCACTTCCCCCATGTACCACAGTGATAAAAACACCGCGAACCAGGAAAAATAAATAATCAGCAGATACAAACCATTGTCTATGGTTTTTCCGACATCCGCACCGTTAAATATTCCGAatgat                       <  1:317030/140‑1 (MQ=255)                                                                                                                 gTTGATCATCATTTTTATCACTTCCCCCATGTACCACAGTGATAAAAACACCGCGAACCAGGAAAAATAAATAATCAGCAGATACAAACCATTGTCTATGGTTTTTCCGACATCCGCACCGTTAAATATTCCGAatgatg                      >  2:782216/1‑140 (MQ=255)                                                                                                                 gTTGATCATCATTTTTATCACTTCCCCCATGTACCACAGTGATAAAAACACCGCGAACCAGGAAAAATAAATAATCAGCAGATACAAACCATTGTCTATGGTTTTTCCGACATCCGCACCGTTAAATATTCCGAatgatg                      >  1:648915/1‑140 (MQ=255)                                                                                                                  ttGATCATCATTTTTATCACTTCCCCCATGTACCACAGTGATAAAAACACCGCGAACCAGGAAAAATAAATAATCAGCAGATACAAACCATTGTCTATGGTTTTTCCGACATCCGCACCGTTAAATATTCCGAATGATGc                     >  2:416374/1‑140 (MQ=255)                                                                                                                  ttGATCATCATTTTTATCACTTCCCCCATGTACCACAGTGATAAAAACACCGCGAACCAGGAAAAATAAATAATCAGCAGATACAAACCATTGTCTATGGTTTTTCCGACATCCGCACCGTTAAATATTCCGAATGATGc                     <  1:1109188/140‑1 (MQ=255)                                                                                                                   tGATCATCATTTTTATCACTTCCCCCATGTACCACAGTGATAAAAACACCGCGAACCAGGAAAAATAAATAATCAGCAGATACAAACCATTGTCTATGGTTTTTCCGACATCCGCACCGTTAAATATTCCGAATGATGCg                    >  2:1064122/1‑140 (MQ=255)                                                                                                                    gATCATCATTTTTATCACTTCCCCCATGTACCACAGTGATAAAAACACCGCGAACCAGGAAAAATAAATAATCAGCAGATACAAACCATTGTCTATGGTTTTTCCGACATCCGCACCGTTAAATATTCCGAATGATGCGa                   <  2:1002635/140‑1 (MQ=255)                                                                                                                    gATCATCATTTTTATCACTTCCCCCATGTACCACAGTGATAAAAACACCGCGAACCAGGAAAAATAAATAATCAGCAGATACAAACCATTGTCTATGGTTTTTCCGACATCCGCACCGTTAAATATTCCGAATGATGCGa                   <  1:588508/140‑1 (MQ=255)                                                                                                                      tcatcaTTTTTATCACTTCCCCCATGTACCACAGTGATAAAAACACCGCGAACCAGGAAAAATAAATAATCAGCAGATACAAACCATTGTCTATGGTTTTTCCGACATCCGCACCGTTAAATATTCCGAATGATGCGACa                 <  2:1284253/140‑1 (MQ=255)                                                                                                                      tcatcaTTTTTATCACTTCCCCCATGTACCACAGTGATAAAAACACCGCGAACCAGGAAAAATAAATAATCAGCAGATACAAACCATTGTCTATGGTTTTTCCGACATCCGCACCGTTAAATATTCCGAATGATGCGACa                 <  2:617358/140‑1 (MQ=255)                                                                                                                        atcatTTTTATCACTTCCCCCATGTACCACAGTGATAAAAACACCGCGAACCAGGAAAAATAAATAATCAGCAGATACAAACCATTGTCTATGGTTTTTCCGACATCCGCACCGTTAAATATTCCGAATGATGCGACata               <  2:1454330/140‑1 (MQ=255)                                                                                                                         tcatTTTTATCACTTCCCCCATGTACCACAGTGATAAAAACACCGCGAACCAGGAAAAATAAATAATCAGCAGATACAAACCATTGTCTATGGTTTTTCCGACATCCGCACCGTTAAATATTCCGAATGATGCGACatat              <  2:1485153/140‑1 (MQ=255)                                                                                                                          catTTTTATCACTTCCCCCATGTACCACAGTGATAAAAACACCGCGAACCAGGAAAAATAAATAATCAGCAGATACAAACCATTGTCTATGGTTTTTCCGACATCCGCACCGTTAAATATTCCGAATGATGCGACa                 >  2:465051/1‑136 (MQ=255)                                                                                                                          catTTTTATCACTTCCCCCATGTACCACAGTGATAAAAACACCGCGAACCAGGAAAAATAAATAATCAGCAGATACAAACCATTGTCTATGGTTTTTCCGACATCCGCACCGTTAAATATTCCGAATGATGCGACa                 <  1:465051/136‑1 (MQ=255)                                                                                                                               ttATCACTTCCCCCATGTACCACAGTGATAAAAACACCGCGAACCAGGAAAAATAAATAATCAGCAGATACAAACCATTGTCTATGGTTTTTCCGACATCCGCACCGTTAAATATTCCGAATGATGCGACATATTCATaa        >  1:1105894/1‑140 (MQ=255)                                                                                                                                 aTCACTTCCCCCATGTACCACAGTGATAAAAACACCGCGAACCAGGAAAAATAAATAATCAGCAGATACAAACCATTGTCTATGGTTTTTCCGACATCCGCACCGTTAAATATTCCGAATGATGCGACATATTCATAAAg      >  2:482564/1‑140 (MQ=255)                                                                                                                                 aTCACTTCCCCCATGTACCACAGTGATAAAAACACCGCGAACCAGGAAAAATAAATAATCAGCAGATACAAACCATTGTCTATGGTTTTTCCGACATCCGCACCGTTAAATATTCCGAATGATGCGACATATTCATAAAg      <  1:482564/140‑1 (MQ=255)                                                                                                                                     cTTCCCCCATGTACCACAGTGATAAAAACACCGCGAACCAGGAAAAATAAATAATCAGCAGATACAAACCATTGTCTATGGTTTTTCCGACATCCGCACCGTTAAATATTCCGAATGATGCGACATATTCATAAAGTGAg  <  1:1064122/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                         GACAGACGATTAAAAATGCATATTCCGGGCTAAATATCGAACCGGTAAAAAACAGCGATACCGGAGTAAAAAGATATAATTGCACGCGAAAATTGCGGTTATCACCAAAAGCGTTGATCATCATTTTTATCACTTTCCCCATGTACCACAGTGATAAAAACACCGCGAACCAGGAAAAATAAATAATCAGCAGATACAAACCATTGTCTATGGTTTTTCCGACATCCGCACCGTTAAATATTCCGAATGATGCGACATATTCATAAAGTGAG  >  NC_000913/2129699‑2129970

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑