Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,568,5040TG27.4% 210.1 / 11.2 106T6P (ACC→CCC) mdtMmultidrug efflux pump/bile salt:H(+) antiporter/Na(+):H(+) antiporter/K(+):H(+) antiporter
Reads supporting (aligned to +/- strand):  ref base T (24/53);  new base G (29/0);  total (53/53)
Fisher's exact test for biased strand distribution p-value = 1.75e-11
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 109 total aligned reads displayed.

CCAGACTGACATCGGCATTAAAATCACGTACCACATTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGG‑GTAAAAAAACGTGGCAT‑AACAAACAACTCCTTGT‑CCGGAG‑AACCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACGAAAGGCAGGTGAGAAGGGCAACGAGACTGCAAATGTACTGAATTTCATGTAATCAAC  >  NC_000913/4568372‑4568643
                                                                                                                                     |                                                                                                                                              
ccAGACTGACATCGGCATTAAAATCACGTACCACATTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGG‑GTaaaaaaa                                                                                                                                         <  1:403406/140‑1 (MQ=255)
ccAGACTGACATCGGCATTAAAATCACGTACCACATTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGG‑GTaaaaaaa                                                                                                                                         <  1:1323356/140‑1 (MQ=255)
  aGACTGACATCGGCATTAAAATCACGTACCACATTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGG‑GTAAAAAAACg                                                                                                                                       <  1:1187680/140‑1 (MQ=255)
    aCTGACATCGGCATTAAAATCACGTACCACATTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGG‑GTaaaaaaaaggg                                                                                                                                     >  1:126724/1‑136 (MQ=255)
    aCTGACATCGGCATTAAAATCACGTACCACATTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGG‑GTAAAAAAACGTg                                                                                                                                     >  1:754994/1‑140 (MQ=255)
     cTGACATCGGCATTAAAATCACGTACCACATTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGG‑GTAAAAAAACGTgg                                                                                                                                    <  1:874391/140‑1 (MQ=255)
       gACATCGGCATTAAAATCACGTACCACATTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGG‑GTAAAAAAACGTGGCa                                                                                                                                  <  1:30454/140‑1 (MQ=255)
       gACATCGGCATTAAAATCACGTACCACATTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGG‑GTAAAAAAACGTGGCa                                                                                                                                  <  1:141526/140‑1 (MQ=255)
       gACATCGGCATTAAAATCACGTACCACATTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGG‑GGAAAAAAAAGAGGCa                                                                                                                                  >  2:39139/1‑140 (MQ=255)
         cATCGGCATTAAAATCACGTACCACATTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGG‑GTAAAAAAACGTGGCAT‑a                                                                                                                               >  2:563036/1‑140 (MQ=255)
         cATCGGCATTAAAATCACGTACCACATTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGG‑GTAAAAAAACGTGGCAT‑a                                                                                                                               <  1:657505/140‑1 (MQ=255)
             ggCATTAAAATCACGTACCACATTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGG‑GGAAAAAAAAAAGGCAT‑aaaaa                                                                                                                           >  2:1118366/1‑140 (MQ=255)
                  tAAAATCACGTACCACATTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGG‑GTAAAAAAACGTGGCAT‑AACAaacaac                                                                                                                      <  2:901668/140‑1 (MQ=255)
                  tAAAATCACGTACCACATTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGG‑GGAAAAAAAAGAGGCAT‑Aaaaaaaaaa                                                                                                                      >  2:43937/1‑139 (MQ=255)
                    aaaTCACGTACCACATTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGG‑GTAAAAAAAAGTGGCAT‑AACAAAAAAATc                                                                                                                    >  2:522062/1‑140 (MQ=255)
                         aCGTACCACATTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGG‑GTAAAAAAAAGGGGCAT‑AACAAAAAAACCCtttt                                                                                                               >  1:317380/1‑138 (MQ=255)
                         aCGTACCACATTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGG‑GGAAAAAAAAGGGGCAT‑AAAAAAAAAATCCTTGt                                                                                                               >  2:991405/1‑140 (MQ=255)
                          cGTACCACATTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGG‑GGGAAAAAACGAGGGAT‑AAAAAACAAATccctttc                                                                                                              >  2:602258/1‑135 (MQ=255)
                               cacaTTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGG‑GTAAAAAAACGTGGCAT‑AACAAACAACTCCTTGT‑CCGgag                                                                                                        <  1:52216/140‑1 (MQ=255)
                                acaTTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGG‑GTAAAAAAACGTGGCAT‑AACAAACAACTCCTTGT‑CCGgag‑a                                                                                                      <  1:602258/140‑1 (MQ=255)
                                 caTTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGG‑GGAAAAAAACGTGGCAT‑AAAAAAAAAATCCTTGT‑CCGGAG‑aa                                                                                                     >  2:1237803/1‑140 (MQ=255)
                                   ttAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGG‑GTAAAAAAACGTGGCAT‑AACAAACAACTCCTTGT‑CCGGAG‑AAcc                                                                                                   <  1:574527/140‑1 (MQ=255)
                                      aTGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGG‑GTAAAAAAAAACGGCAT‑AAAAAAAAAATCCTTTT‑CCGGAG‑AAccccc                                                                                                >  2:573243/1‑139 (MQ=255)
                                       tGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGG‑GTAAAAAAACGTGGCAT‑AACAAACAACTcc                                                                                                                   <  2:1214329/122‑1 (MQ=255)
                                       tGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGG‑GTAAAAAAAAGTGGCAT‑AACAAACAACTcc                                                                                                                   >  1:1214329/1‑122 (MQ=255)
                                         aTCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGG‑GTAAAAAAAAGTGGCAT‑AACAAAAAAATCCTTTG‑CCGGAGAAACCCCaag                                                                                              >  2:628839/1‑139 (MQ=255)
                                           cccAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGAGTAAAAAAA‑GCGGCAC‑AAAAAACAAATCCTTAT‑CCCGAG‑GAACCCAACggg                                                                                           >  2:541777/1‑140 (MQ=255)
                                           cccAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGG‑GTAAAAAAACGTGGCAT‑AACAAACAACTCCTTGT‑CCGGAG‑AACCCCAACggg                                                                                           >  1:1234628/1‑140 (MQ=255)
                                           cccAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGG‑GTAAAAAAACGTGGCAT‑AACAAACAACTCCTTGT‑CCGGAG‑AACCCCAACggg                                                                                           >  1:1141885/1‑140 (MQ=255)
                                           cccAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGG‑GTAAAAAAAAGTGGCAT‑AACAAAAAAATCCTTGT‑CCGGAG‑AACCCCCACggg                                                                                           >  1:183515/1‑140 (MQ=255)
                                           cccAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGG‑GGAAAAAAACGGGGGAG‑AAAAAACAACTCCTTTT‑CCGGAG‑AACCCCCACggg                                                                                           >  1:765404/1‑140 (MQ=255)
                                            ccAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGG‑GGAAAAAAAAGTGGCAT‑AACAAACAACTCCTTGT‑CCGGAG‑AACCCCAACgggg                                                                                          >  2:1455133/1‑140 (MQ=255)
                                            ccAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGG‑GGAAAAAAAAGTGGCAT‑AAAAAAAAACTCCCTGT‑CCCGAG‑AAACCCAACgggg                                                                                          >  2:1341599/1‑140 (MQ=255)
                                               ggCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGG‑GGAAAAAAAAGTGGCAT‑AAAAAACAACCCCCTGT‑CCGGGG‑GACCCCAACCGGGgaa                                                                                       >  2:587569/1‑140 (MQ=255)
                                                gCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGG‑GTAAAAAAAAGTGGCAT‑AACAAACAACTCCCTTT‑CCGGAG‑AACCCCAACGGGGaaaa                                                                                      >  1:567657/1‑140 (MQ=255)
                                                gCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGG‑GGAAAAAAACATGGGAT‑AAAAAAAAACACCTTGT‑CCGGAG‑AACCCCCACGGGGgaaa                                                                                      >  2:1225411/1‑140 (MQ=255)
                                                  tGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGG‑GTAAAAAAACGTGGCAT‑AACAAACAACTCCTTGT‑CCGGAG‑AACCCCAACGGGGAAAACg                                                                                    <  1:398164/140‑1 (MQ=255)
                                                  tGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGG‑GTAAAAAAACGTGGCAT‑AACAAACAACTCCTTGT‑CCGGAG‑AACCCCAACGGGGAAAACg                                                                                    <  1:991405/140‑1 (MQ=255)
                                                  tGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGG‑GTAAAAAAACGTGGCAT‑AACAAACAACTCCTTGT‑CCGGAG‑AACCCCAACGGGGAAAACg                                                                                    <  2:88154/140‑1 (MQ=255)
                                                  tGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGG‑GTAAAAAAACGTGGCAT‑AACAAACAACTCCTTGT‑CCGGAG‑AACCCCAACGGGGAAAACg                                                                                    <  1:1041873/140‑1 (MQ=255)
                                                    gATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGG‑GGAAAAAAAAGTGGCAT‑AAAAAAAAACTCCTTGT‑CCGGAG‑AAACCCCACCGGGAAAAAGaa                                                                                  >  2:933318/1‑140 (MQ=255)
                                                     aTCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGG‑GTAAAAAAACGTGGCAT‑AACAAACAACTCCTTGT‑CCGGAG‑AACCCCAACGGGGAAAACGaaa                                                                                 <  2:1234628/140‑1 (MQ=255)
                                                     aTCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGG‑GTAAAAAAAAGGGGCAT‑AAAAAAAAACTCCCTGT‑CCGGAG‑AACCCCAACGGGGGAAACGaaa                                                                                 >  1:295754/1‑140 (MQ=255)
                                                     aTCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGG‑GGAAAAAAACGTGGCAT‑AAAAAACAACTCCTTGT‑CCGGAG‑AACCCCAACGGGGAAAACGaaa                                                                                 >  1:1140733/1‑140 (MQ=255)
                                                     aTCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGG‑GGAAAAAAAAGTGGCAT‑AACAAAAAACTCCTTGT‑CCGGAG‑AACCCCAACGGGGgaaaaaaaa                                                                                 >  2:1169152/1‑135 (MQ=255)
                                                     aTCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGG‑GGAAAAAAAAGAGGGCT‑AAAAAAAAACTCCCTTT‑CCGGGG‑AAACCCCACGGGGGAAAAGaaa                                                                                 >  2:276541/1‑140 (MQ=255)
                                                         gATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGG‑GTAAAAAAAAGTGGCAT‑AACAAAAAACTCCTTTT‑CCGGAG‑AACCCCAAAGGGGAAAAAGAAAATCa                                                                             >  1:1150666/1‑140 (MQ=255)
                                                          aTCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGG‑GTAAAAAAAAGGGGCAT‑AAAAAAAAACTCCTTTT‑CCGGAG‑AACCCCAACGGGGAAAAAGAAATTCaa                                                                            >  1:1284644/1‑140 (MQ=255)
                                                          aTCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGG‑GGAAAAAAAAGTGGCAT‑AACAAACAAATCCTTGT‑CCGGAG‑AACCCCCACGGGGAAAAAGAAATTCaa                                                                            >  2:1174511/1‑140 (MQ=255)
                                                              gTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGG‑GTAAAAAAACGTGGCAT‑AACAAACAACTCCTTGT‑CCGGAG‑AACCCCAACGGGGAAAACGAAATTCAATAcc                                                                        <  1:1462854/140‑1 (MQ=255)
                                                              gTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGG‑GTAAAAAAACGTGGCAT‑AACAAACAACTCCTTGT‑CCGGAG‑AACCCCAACGGGGAAAACGAAATTCAATAcc                                                                        <  2:1192211/140‑1 (MQ=255)
                                                               tCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGG‑GTAAAAAAACGTGGCAT‑ATCAAACAACTCCTTGT‑CCGGAG‑AACCCCAACGGGGAAAACGAAATTCAATACCg                                                                       <  1:543395/140‑1 (MQ=255)
                                                               tCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGG‑GTAAAAAAACGTGGCAT‑AACAAACAACTCCTTGT‑CCGGAG‑AACCCCAACGGGGAAAACGAAATTCAATACCg                                                                       <  1:628839/140‑1 (MQ=255)
                                                               tCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGG‑GTAAAAAAAAGTGGCAT‑AAAAAACAACTCCTTGT‑CCGGAG‑AACCCCAACAGGGGAAAAGAAAATCAATAccc                                                                       >  2:420091/1‑139 (MQ=255)
                                                               aggACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGG‑GTAAAAAAACGTGGCAT‑AACAAACAACTCCTTGT‑CCGGAG‑AACCCCAACGGGGAAAACGAAATTCAATACCg                                                                       <  1:445581/138‑1 (MQ=255)
                                                                cGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGG‑GGAAAAAAAAGAGGGATAAAAAAAAAACACCTT‑T‑CCGGGG‑GAACCCAACGGGGGAAAAGAAAATAAATACCGt                                                                      >  2:513231/1‑140 (MQ=255)
                                                                  tatctgtcGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGG‑GTAAAAAAACGTGGCAT‑AACAAACAACTCCTTGT‑CCGGAG‑AACCCCAACGGGGAAAACGAAATTCAATACCGTCg                                                                    >  1:1046533/8‑140 (MQ=255)
                                                                    aGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGG‑GGAAAAAAAAGTGGCAT‑AACAAACAACTCCTTGT‑CCCGAG‑AACCCCCACGGGGAAAAAGAAATTCAATAACCTCggg                                                                  >  1:914029/1‑139 (MQ=255)
                                                                    aGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGG‑GGAAAAAAAAGTGGCAT‑AACAAAAAACTCCTTGT‑CCGGAG‑AACCCCCACGGGGAAAAAGAAAATCAATACCGTCggg                                                                  >  1:324686/1‑139 (MQ=255)
                                                                      aTACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGG‑GTAAAAAAACGTGGCAT‑AACAAACAACTCCTTGT‑CCGGAG‑AACCCCAACGGGGAAAACGAAATTCAATACCGTCGGTaa                                                                <  1:933318/140‑1 (MQ=255)
                                                                      aTACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGG‑GTAAAAAAACGTGGCAT‑AACAAACAACTCCTTGT‑CCGGAG‑AACCCCAACGGGGAAAACGAAATTCAATACCGTCGGTaa                                                                <  1:573243/140‑1 (MQ=255)
                                                                            agcaAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGG‑GGAAAAAAAAGTGGCAT‑AAAAAAAAACTCCTTGT‑CCGGGG‑AAACCCAACGGGGAAAAAGAAAATCAATAACGTCGGGAAAGAccc                                                          >  2:445581/1‑138 (MQ=255)
                                                                              caAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGG‑GTAAAAAAACGTGGCAT‑AACAAACAACTCCTTGT‑CCGGAG‑AACCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACGaaa                                                        <  2:507361/140‑1 (MQ=255)
                                                                              caAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGG‑GTAAAAAAACGTGGCAT‑AACAAACAACTCCTTGT‑CCGGAG‑AACCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACGaaa                                                        <  2:434477/140‑1 (MQ=255)
                                                                              caAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGG‑GTAAAAAAACGTGGCAT‑AACAAACAACTCCTTGT‑CCGGAG‑AACCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACGaaa                                                        <  2:939409/140‑1 (MQ=255)
                                                                              caAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGG‑GGAAAAAAAAGTGGCAT‑AAAAAAAAACTCCTTGT‑CCGGAG‑AACCCCAACGGGGAAAAAGAAAATCAAAACCCTCGGTAATGACGaaa                                                        >  2:77915/1‑140 (MQ=255)
                                                                                aaGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGG‑GTAAAAAAACGTGGCAT‑AACAAACAACTCCTTGT‑CCGGAG‑AACCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACGAAAgg                                                      <  2:795/140‑1 (MQ=255)
                                                                                aaGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGG‑GTAAAAAAACGTGGCAT‑AACAAACAACTCCTTGT‑CCGGAG‑AACCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACGAAAgg                                                      <  1:1237803/140‑1 (MQ=255)
                                                                                aaGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGG‑GTAAAAAAACGTGGCAT‑AACAAACAACTCCTTGT‑CCGGAG‑AACCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACGAAAgg                                                      <  1:1225411/140‑1 (MQ=255)
                                                                                aaGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGG‑GGAAAAAAAAGTGGCAT‑AAAAAAAAACTCCTTGT‑CCGGAG‑AACCCCCACGGGGGAAAAGAAAATCAATACCGTCGGTGATGACGAAAgg                                                      >  2:416591/1‑140 (MQ=255)
                                                                                       aCAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGG‑GTAAAAAAACGTGGCAT‑AACAAACAACTCCTTGT‑CCGGAG‑AACCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACGAAAGGCAGGTga                                               <  2:1211680/140‑1 (MQ=255)
                                                                                        cAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGG‑GTAAAAAAACGTGGCAT‑AACAAACAACTCCTTGT‑CCGGAG‑AACCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACGAAAGGCAGGTgag                                              <  1:530074/140‑1 (MQ=255)
                                                                                           aaTCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGG‑GTAAAAAAACGTGGCAT‑AACAAACAACTCCTTGT‑CCGGAG‑AACCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACGAAAGGCAGGTGAGAAg                                           <  1:513231/140‑1 (MQ=255)
                                                                                             tCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGG‑GGAAAAAAAAGTGGGAT‑AAAAAAAAAAACCTTTT‑CCGGAG‑AACCCCCACGGGGAAAACGAAAATCAAAAACGCCGGTAAAGAAGAAAAGGAGGGGAGAAagg                                         >  2:718127/1‑140 (MQ=255)
                                                                                                aCGCCATCGGGAAAAACAGCGTGGCGGCATGGCGG‑GTAAAAAAACGTGGCAT‑AACAAACAACTCCTTGT‑CCGGAG‑AACCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACGAAAGGCAGGTGAGAAGGGCaa                                      <  1:563036/140‑1 (MQ=255)
                                                                                                aCGCCATCGGGAAAAACAGCGTGGCGGCATGGCGG‑GGAAAAAAAAGTGGCAT‑AAAAAAAAAATCCTTGT‑CCGGAG‑AAACCCCACCGGGAAAAAGAAAATCAAAACCCTCGGTAAAGACGAAAAGAAGGGGAGAAGGGCaa                                      >  2:203029/1‑140 (MQ=255)
                                                                                                 cGCCATCGGGAAAAACAGCGTGGCGGCATGGCGG‑GTAAAAAAACGTGGCAT‑AACAAACAACTCCTTGT‑CCGGAG‑AACCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACGAAAGGCAGGTGAGAAGGGCAAc                                     <  1:304756/140‑1 (MQ=255)
                                                                                                 cGCCATCGGGAAAAACAGCGTGGCGGCATGGCGG‑GTAAAAAAACGTGGCAT‑AACAAACAACTCCTTGT‑CCGGAG‑AACCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACGAAAGGCAGGTGAGAAGGGCAAc                                     <  1:1341599/140‑1 (MQ=255)
                                                                                                   ccATCGGGAAAAACAGCGTGGCGGCATGGCGG‑GTAAAAAAACGTGGCAT‑AACAAACAACTCCTTGT‑CCGGAG‑AACCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACGAAAGGCAGGTGAGAAGGGCAACga                                   <  1:203029/140‑1 (MQ=255)
                                                                                                   ccATCGGGAAAAACAGCGTGGCGGCATGGCGG‑GTAAAAAAACGTGGCAT‑AACAAACAACTCCTTGT‑CCGGAG‑AACCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACGAAAGGCAGGTGAGAAGGGCAACga                                   <  2:1046533/140‑1 (MQ=255)
                                                                                                   ccATCGGGAAAAACAGCGTGGCGGCATGGCGG‑GTAAAAAAACGTGGCAT‑AACAAACAACTCCTTGT‑CCGGAG‑AACCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACGAAAGGCAGGTGAGAAGGGCAACga                                   <  2:316022/140‑1 (MQ=255)
                                                                                                     aTCGGGAAAAACAGCGTGGCGGCATGGCGG‑GTAAAAAAAAGTGGCAT‑AAAAAAAAACTCCTTGT‑CCGGAG‑AAACCCAACGGGGGAAACGAAAATCAAAAACCTCGGTAAAGACGAAAAGCAGGGGGGGAGGGGAACgaga                                 >  2:966291/1‑140 (MQ=255)
                                                                                                           aaaaaCAGCGTGGCGGCATGGCGG‑GGAAAAAAAAGTGGCAT‑AAAAAACAAACCCTTGT‑CCGGAG‑AACCCCAACGGGGGAAAAGAAAATCAATACCGTCGGTAATGACGAAAAGCAGGGGGGAAGGGCAACGAGACTGCaa                           >  2:4703/1‑140 (MQ=255)
                                                                                                                cgggttGGCGGCATGGCGG‑GT‑AAAAAACGTGGCAT‑AACAAACAACTCCTTGT‑CCGGAG‑AACCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACGAAAGGCAGGTGAGAAGGGCAACGAGACTGCAAATGTAc                     <  2:1284644/135‑1 (MQ=255)
                                                                                                                  gCGTGGCGGCATGGCGG‑GTAAAAAAACGTGGCAT‑AACAAACAACTCCTTGT‑CCGGAG‑AACCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACGAAAGGCAGGTGAGAAGGGCAACGAGACTGCAAATGTACt                    <  1:1118366/140‑1 (MQ=255)
                                                                                                                  gCGTGGCGGCATGGCGG‑GTAAAAAAACGTGGCAT‑AACAAACAACTCCTTGT‑CCGGAG‑AACCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACGAAAGGCAGGTGAGAAGGGCAACGAGACTGCAAATGTACt                    <  1:1169152/140‑1 (MQ=255)
                                                                                                                  gCGTGGCGGCATGGCGG‑GTAAAAAAACGTGGCAT‑AACAAACAACTCCTTGT‑CCGGAG‑AACCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACGAAAGGCAGGTGAGAAGGGCAACGAGACTGCAAATGTACt                    <  1:1336375/140‑1 (MQ=255)
                                                                                                                  gCGTGGCGGCATGGCGG‑GTAAAAAAACGTGGCAT‑AACAAACAACTCCTTGT‑CCGGAG‑AACCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACGAAAGGCAGGTGAGAAGGGCAACGAGACTGCAAATGTACt                    <  2:765404/140‑1 (MQ=255)
                                                                                                                  gCGTGGCGGCATGGCGG‑GGAAAAAAACAGGGCAT‑AAAAAACAACACCCTGT‑CCCGAG‑AACCCCCACGGGGAAAACGAAAATCAATACCGTCGGGAAAGACGAAAAGCAGGGGAGAAAGGCAACGAAACTGCAAAAGTaat                    >  2:711204/1‑138 (MQ=255)
                                                                                                                    gTGGCGGCATGGCGG‑GGAAAAAAAAGTGGCAT‑AAAAAACAACTCCTTGT‑TCGGGG‑AACCCCCACGGGGAAAACGAAATTCAAAACCGTCGGTAATGACGAAAAGCAGGGGGGAAAGGGAACGAGACTGCAAAAGTAATGa                  >  2:769735/1‑140 (MQ=255)
                                                                                                                        cggcATGGCGG‑GTAAAAAAAAGTGGCAT‑AACAAACAACTCCTTGT‑CCGGAG‑AACCCCAACGGGGAAAACGAAATTCAATACCGTCGGGAATGACGAAAGGCAGGTGAGAAGGGCAACGAGACTGCAAATGTACTGAAttt              >  1:288526/1‑140 (MQ=255)
                                                                                                                        cggcATGGCGG‑GTAAAAAAAAGTGGCAT‑AAAAAACAACTCCTTGT‑CCGGAG‑AACCCCCACGGGGGAAACGAAATTCAATACCGTCGGGAATGACGAAAAGCAGGGGAGAAGGGGAACGAGACTGCAAAAGTACTGAAttt              >  2:998323/1‑140 (MQ=255)
                                                                                                                            aTGGCGG‑GTAAAAAAACGTGGCAT‑AACAAACAACTCCTTGT‑CCGGAG‑AACCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACGAAAGGCAGGTGAGAAGGGCAACGAGACTGCAAATGTACTGAATTTCATg          <  1:276541/140‑1 (MQ=255)
                                                                                                                            aTGGCGG‑GTAAAAAAACGTGGCAT‑AACAAACAACTCCTTGT‑CCGGAG‑AACCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACGAAAGGCAGGTGAGAAGGGCAACGAGACTGCAAATGTACTGAATTTCATg          <  2:1150666/140‑1 (MQ=255)
                                                                                                                             tGGCGG‑GTAAAAAAACGTGGGAT‑AAAAAACAAACCCTTGTCCCGGGG‑ACCCCCAAGGGGGAAAA‑GAAAATCAATACCGGCGGGAAATACGAAAGGCAGGGGGGGAAGGGAACGAGAATGCCAAAGGAATGAATTTCATGt         >  2:1110856/1‑140 (MQ=255)
                                                                                                                              ggCGG‑GTAAAAAAACGTGGCAT‑AACAAACAACTCCTTGT‑TCGGAG‑AACCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACGAAAGGCAGGTGAGAAGGGCAACGAGACTGCAAATGTACTGAATTTCATGTa        <  1:172716/140‑1 (MQ=255)
                                                                                                                              ggCGG‑GTAAAAAAACGTGGCAT‑AACAAACAACTCCTTGT‑CCGGAG‑AACCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACGAAAGGCAGGTGAGAAGGGCAACGAGACTGCAAATGTACTGAATTTCATGTa        <  1:1363315/140‑1 (MQ=255)
                                                                                                                              ggCGG‑GTAAAAAAACGTGGCAT‑AACAAACAACTCCTTGT‑CCGGAG‑AACCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACGAAAGGCAGGTGAGAAGGGCAACGAGACTGCAAATGTACTGAATTTCATGTa        <  1:416591/140‑1 (MQ=255)
                                                                                                                              ggCGG‑GTAAAAAAACGTGGCAT‑AACAAACAACTCCTTGT‑CCGGAG‑AACCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACGAAAGGCAGGTGAGAAGGGCAACGAGACTGCAAATGTACTGAATTTCATGTa        <  1:77915/140‑1 (MQ=255)
                                                                                                                              ggCGG‑GTAAAAAAACGTGGCAT‑AACAAACAACTCCTTGT‑CCGGAG‑AACCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACGAAAGGCAGGTGAGAAGGGCAACGAGACTGCAAATGTACTGAATTTCATGTa        <  1:801008/140‑1 (MQ=255)
                                                                                                                                     |                                                                                                                                              
CCAGACTGACATCGGCATTAAAATCACGTACCACATTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGG‑GTAAAAAAACGTGGCAT‑AACAAACAACTCCTTGT‑CCGGAG‑AACCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACGAAAGGCAGGTGAGAAGGGCAACGAGACTGCAAATGTACTGAATTTCATGTAATCAAC  >  NC_000913/4568372‑4568643

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑