Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,786,901	T→C	E71G (GAA→GGA)	ppsA ←	phosphoenolpyruvate synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,786,901	0	T	C	100.0%	217.3 / NA	56	E71G (GAA→GGA)	ppsA	phosphoenolpyruvate synthetase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (30/26);  total (30/26)

TGTGCATAGGCTTCGCGGATGGCGTTTTCCAGCTCAGGCTGGAAGGGAGTGTCGATAATCCACTGGCGGATTTGCGCGCCCGCTTTCGCAAGCTGAGTAACATCGTCAATATCCGTTTTATCCAGCAGTTCATAAATGCGCTGGTTTACGCCGCTTTGGTCCAGAAACTGGTTAAACGCGTCGGCGGTTGTGGCGAAACCATTCGGAACGGAAACACCCATTCCGGAAAGATTAGTAATCATTTCACCCAGGGAGGCATTTTTGC  >  NC_000913/1786772‑1787036                                                                                                                                  |                                                                                                                                        tgtgCATAGGCTTCGCGGATGGCGTTTTCCAGCTCAGGCTGGAAGGGAGTGTCGATAATCCACTGGCGGATTTGCGCGCCCGCTTTCGCAAGCTGAGTAACATCGTCAATATCCGTTTTATCCAGCAGTCCATAAATgcg                                                                                                                               <  1:416410/140‑1 (MQ=255)    gCATAGGCTTCGCGGATGGCGTTTTCCAGCTCAGGCTGGAAGGGAGTGTCGATAATCCACTGGCGGATTTGCGCGCCCGCTTTCGCAAGCTGAGTAACATCGTCAATATCCGTTTTATCCAGCAGTCCATAAATGCGCTg                                                                                                                            <  2:1161725/140‑1 (MQ=255)       tAGGCTTCGCGGATGGCGTTTTCCAGCTCAGGCTGGAAGGGAGTGTCGATAATCCACTGGCGGATTTGCGCGCCCGCTTTCGCAAGCTGAGTAACATCGTCAATATCCGTTTTATCCAGCAGTCCATAAATGCGCTGGtt                                                                                                                         <  2:425319/140‑1 (MQ=255)        aGGCTTCGCGGATGGCGTTTTCCAGCTCAGGCTGGAAGGGAGTGTCGATAATCCACTGGCGGATTTGCGCGCCCGCTTTCGCAAGCTGAGTAACATCGTCAATATCCGTTTTATCCAGCAGTCCATAAATGCGCTGGttt                                                                                                                        >  1:825077/1‑140 (MQ=255)          gCTTCGCGGATGGCGTTTTCCAGCTCAGGCTGGAAGGGAGTGTCGATAATCCACTGGCGGATTTGCGCGCCCGCTTTCGCAAGCTGAGTAACATCGTAAATATCCGTTTTATCCAGCAGTCCATAAATGCGCTGGTTTAc                                                                                                                      >  1:459002/1‑140 (MQ=255)             tCGCGGATGGCGTTTTCCAGCTCAGGCTGGAAGGGAGTGTCGATAATCCACTGGCGGATTTGCGCGCCCGCTTTCGCAAGCTGAGTAACATCGTCAATATCCGTTTTATCCAGCAGTCCATAAATGCGCTGGTTTAcgcc                                                                                                                   >  2:1405289/1‑140 (MQ=255)             tCGCGGATGGCGTTTTCCAGCTCAGGCTGGAAGGGAGTGTCGATAATCCACTGGCGGATTTGCGCGCCCGCTTTCGCAAGCTGAGTAACATCGTCAATATCCGTTTTATCCAGCAGTCCATAAATGCGCTGGTTTAcgcc                                                                                                                   >  1:231513/1‑140 (MQ=255)             tCGCGGATGGCGTTTTCCAGCTCAGGCTGGAAGGGAGTGTCGATAATCCACTGGCGGATTTGCGCGCCCGCTTTCGCAAGCTGAGTAACATCGTCAATATCCGTTTTATCCAGCAGTCCATAAATGCGCTGGTTTAcgcc                                                                                                                   >  1:491194/1‑140 (MQ=255)             tCGCGGATGGCGTTTTCCAGCTCAGGCTGGAAGGGAGTGTCGATAATCCACTGGCGGATTTGCGCGCCCGCTTTCGCAAGCTGAGTAACATCGTCAATATCCGTTTTATCCAGCAGTCCATAAATGCGCTGGTTTAcgcc                                                                                                                   >  2:897385/1‑140 (MQ=255)                cgGATGGCGTTTTCCAGCTCAGGCTGGAAGGGAGTGTCGATAATCCACTGGCGGATTTGCGCGCCCGCTTTCGCAAGCTGGGTAACATCGTCAATATCCGTTTTATCCAGCAGTCCATAAATGCGCTGGTTTACGCCGCt                                                                                                                >  1:993280/1‑140 (MQ=255)                cgGATGGCGTTTTCCAGCTCAGGCTGGAAGGGAGTGTCGATAATCCACTGGCGGATTTGCGCGCCCGCTTTCGCAAGCTGAGTAACATCGTCAATATCCGTTTTATCCAGCAGTCCATAAATGCGCTGGTTTACGCCGCt                                                                                                                <  1:897385/140‑1 (MQ=255)                 ggATGGCGTTTTCCAGCTCAGGCTGGAAGGGAGTGTCGATAATCCACTGGCGGATTTGCGCGCCCGCTTTCGCAAGCTGAGTAACATCGTCAATATCCGTTTTATCCATCAGTCCATAAATGCGCTGGTTTACGCCGCtt                                                                                                               <  1:876561/140‑1 (MQ=255)                    tGGCGTTTTCCAGCTCAGGCTGGAAGGGAGTGTCGATAATCCACTGGCGGATTTGCGCGCCCGCTTTCGCAAGCTGAGTAACATCGTCAATATCCGTTTTATCCAGCAGTCCATAAATGCGCTGGTTTACGCCGCTTTgg                                                                                                            >  1:762268/1‑140 (MQ=255)                            tCCAGCTCAGGCTGGAAGGGAGTGTCGATAATCCACTGGCGGATTTGCGCGCCCGCTTTCGCAAGCTGAGTAACATCGTCAATATCCGTTTTATCCAGCAGTCCATAAATGCGCTGGTTTACGCCGCTTTGGTCCAGaaa                                                                                                    <  2:279840/140‑1 (MQ=255)                                    aGGCTGGAAGGGAGTGTCGATAATCCACTGGCGGATTTGCGCGCCCGCTTTCGCAAGCTGAGTAACATCGTCAATATCCGTTTTATCCAGCAGTCCATAAATGCGCTGGTTTACGCCGCTTTGGTCCAGAAACTGGTTaa                                                                                            >  1:317168/1‑140 (MQ=255)                                           aaGGGAGTGTCGATAATCCACTGGCGGATTTGCGCGCCCGCTTTCGCAAGCTGAGTAACATCGTCAATATCCGTTTTATCCAGCAGTCCATAAATGCGCTGGTTTACGCCGCTTTGGTCCAGa                                                                                                      <  1:1336987/123‑1 (MQ=255)                                           aaGGGAGTGTCGATAATCCACTGGCGGATTTGCGCGCCCGCTTTCGCAAGCTGAGTAACATCGTCAATATCCGTTTTATCCAGCAGTCCATAAATGCGCTGGTTTACGCCGCTTTGGTCCAGa                                                                                                      >  2:1336987/1‑123 (MQ=255)                                           aaGGGAGTGTCGATAATCCACTGGCGGATTTGCGCGCCCGCTTTCGCAAGCTGAGTAACATCGTCAATATCCGTTTTATCCAGCAGTCCATAAATGCGCTGGTTTACGCCGCTTTGGTCCAGAAACTGGTTAAACGCGTc                                                                                     >  1:894101/1‑140 (MQ=255)                                           aaGGGAGTGTCGATAATCCACTGGCGGATTTGCGCGCCCGCTTTCGCAAGCTGAGTAACATCGTCAATATCCGTTTTATCCAGCAGTCCATAAATGCGCTGGTTTACGCCGCTTTGGTCCAGAAACTGGTTAAACGCGTc                                                                                     >  1:790613/1‑140 (MQ=255)                                             gggAGTGTCGATAATCCACTGGCGGATTTGCGCGCCCGCTTTCGCAAGCTGAGTAACATCGTCAATATCCGTTTTATCCAGCAGTCCATAAATGCGCTGGTTTACGCCGCTTTGGTCCAGAAACTGGTTAAACGCGTcgg                                                                                   <  2:86574/140‑1 (MQ=255)                                             gggAGTGTCGATAATCCACTGGCGGATTTGCGCGCCCGCTTTCGCAAGCTGAGTAACATCGTCAATATCCGTTTTATCCAGCAGTCCATAAATGCGCTGGTTTACGCCGCTTTGGTCCAGAAACTGGTTAAACGCGTcgg                                                                                   <  2:762268/140‑1 (MQ=255)                                                aGTGTCGATAATCCACTGGCGGATTTGCGCGCCCGCTTTCGCAAGCTGAGTAACATCGTCAATATCCGTTTTATCCAGCAGTCCATAAATGCGCTGGTTTACGCCGCTTTGGTCCAGAAACTGGTTAAACGCGTcggcgg                                                                                >  2:83623/1‑140 (MQ=255)                                                aGTGTCGATAATCCACTGGCGGATTTGCGCGCCCGCTTTCGCAAGCTGAGTAACATCGTCAATATCCGTTTTATCCAGCAGTCCATAAATGCGCTGGTTTACGCCGCTTTGGTCCAGAAACTGGTTAAACGCGTcggcgg                                                                                >  1:1475031/1‑140 (MQ=255)                                                  tgtCGATAATCCACTGGCGGATTTGCGCGCCCGCTTTCGCAAGCTGAGTAACATCGTCAATATCCGTTTTATCCAGCAGTCCATAAATGCGCTGGTTTACGCCGCTTTGGTCCAGAAACTGGTTAAACGCGTCGGCGGtt                                                                              <  2:963323/140‑1 (MQ=255)                                                  tgtCGATAATCCACTGGCGGATTTGCGCGCCCGCTTTCGCAAGCTGAGTAACATCGTCAATATCCGTTTTATCCAGCAGTCCATAAATGCGCTGGTTTACGCCGCTTTGGTCCAGAAACTGGTTAAACGCGTCGGCGGtt                                                                              >  1:963323/1‑140 (MQ=255)                                                    tCGATAATCCACTGGCGGATTTGCGCGCCCGCTTTCGCAAGCTGAGTAACATCGTCAATATCCGTTTTATCCAGCAGTCCATAAATGCGCTGGTTTACGCCGCTTTGGTCCAGAAACTGGTTaaa                                                                                           >  1:558581/1‑125 (MQ=255)                                                    tCGATAATCCACTGGCGGATTTGCGCGCCCGCTTTCGCAAGCTGAGTAACATCGTCAATATCCGTTTTATCCAGCAGTCCATAAATGCGCTGGTTTACGCCGCTTTGGTCCAGAAACTGGTTaaa                                                                                           <  2:558581/125‑1 (MQ=255)                                                    tCGATAATCCACTGGCGGATTTGCGCGCCCGCTTTCGCAAGCTGAGTAAAATCGGCAATATCCGTTTTATCCAGCAGTCCATAAATGCGCTGGTTTACGCCGCTTTGGTCCAGAAACTGGTTAAACGCGTCGGCGGTtgg                                                                            >  1:717622/1‑139 (MQ=255)                                                      gATAATCCACTGGCGGATTTGCGCGCCCGCTTTCGCAAGCTGAGTAACATCGTCAATATCCGTTTTATCCAGCAGTCCATAAATGCGCTGGTTTACGCCGCTTTGGTCCAGAAACTGGTTAAACGCGTCGGCGGTTGTgg                                                                          <  1:1213719/140‑1 (MQ=255)                                                      gATAATCCACTGGCGGATTTGCGCGCCCGCTTTCGCAAGCTGAGTAACATCGTCAATATCCGTTTTATCCAGCAGTCCATAAATGCGCTGGTTTACGCCGCTTTGGTCCAGAAACTGGTTAAACGCGTCGGCGGTTGTgg                                                                          <  2:993280/140‑1 (MQ=255)                                                         aaTCCACTGGCGGATTTGCGCGCCCGCTTTCGCAAGCTGAGTAACATCGTCAATATCCGTTTTATCCAGCAGTCCATAAATGCGCTGGTTTACGCCGCTTTGGTCCAGAAACTGGTTAAACGCGTCGGCGGTTGTGGCGa                                                                       >  1:338470/1‑140 (MQ=255)                                                          aTCCACTGGCGGATTTGCGCGCCCGCTTTCGCAAGCTGAGTAACATCGTCAATATCCGTTTTATCCAGCAGTCCATAAATGCGCTGGTTTACGCCGCTTTGGTCCAGAAACTGGTTAAACGCGTCGGCGGTTGTGGCGaa                                                                      <  2:825077/140‑1 (MQ=255)                                                                tGGCGGATTTGCGCGCCCGCTTTCGCAAGCTGAGTAACATCGTCAATATCCGTTTTATCCAGCAGTCCATAAATGCGCTGGTTTACGCCGCTTTGGTCCAGAAACTGGTTAAACGCGTCGGCGGTTGTGGCGAAACCAtt                                                                <  1:1461721/140‑1 (MQ=255)                                                                tGGCGGATTTGCGCGCCCGC‑TTCGCAAGCTGAGTAACATCGTCAATATCCGTTTTATCCAGCAGTCCATAAATGCGCTGGTTTACGCCGCTTTGGTCCAGAAACTGGTTAAACGCGTCGGCGGTTGTGGCGaaa                                                                     >  1:1262/1‑134 (MQ=255)                                                                tGGCGGATTTGCGCGCCCGC‑TTCGCAAGCTGAGTAACATCGTCAATATCCGTTTTATCCAGCAGTCCATAAATGCGCTGGTTTACGCCGCTTTGGTCCAGAAACTGGTTAAACGCGTCGGCGGTTGTGGCGaaa                                                                     <  2:1262/134‑1 (MQ=255)                                                                     gATTTGCGCGCCCGCTTTCGCAAGCTGAGTAACATCGTCAATATCCGTTTTATCCAGCAGTCCATAAATGCGCTGGTTTACGCCGCTTTGGTCCAGAAACTGGTTAAACGCGTCGGCGGTTGTGGCGAAACCATTcggaa                                                           <  1:1411577/140‑1 (MQ=255)                                                                     gATTTGCGCGCCCGCTTTCGCAAGCTGAGTAACATCGTCAATATCCGTTTTATCCAGCAGTCCATAAATGCGCTGGTTTACGCCGCTTTGGTCCAGAAACTGGTTAAACGCGTCGGCGGTTGTGGCGAAACCATTcggaa                                                           <  2:790613/140‑1 (MQ=255)                                                                         tGCGCGCCCGCTTTCGCAAGCTGAGTAACATCGTAAATATCCGTTTTATCCAGCAGTCCATAAATGCGCTGGTTTACACCGCTTTGGTCCAGAAACTGGTTAAACGCGTCGGCGGTTGTGGCGAAACCATTcggaacgga                                                       <  2:459002/140‑1 (MQ=255)                                                                                     ttCGCAAGCTGAGTAACATCGTCAATATCCGTTTTATCCAGCAGTCCATAAATGCGCTGGTTTACGCCGCTTTGGTCCAGAAACTGGTTAAACGCGTCGGCGGTTGTGGCGAAACCATTCGGAACGGAAACACCCATTcc                                           <  2:182838/140‑1 (MQ=255)                                                                                        gCAAGCTGAGTAACATCGTCAATATCCGTTTTATCCAGCAGTCCATAAATGCGCTGGTTTACGCCGCTTTGGTCCAGAAACTGGTTAAACGCGTCGGCGGTTGTGGCGAAACCATTCGGAACGGAAACACCCATTCCGGa                                        >  2:1113689/1‑140 (MQ=255)                                                                                        gCAAGCTGAGTAACATCGTCAATATCCGTTTTATCCAGCAGTCCATAAATGCGCTGGTTTACGCCGCTTTGGTCCAGAAACTGGTTAAACGCGTCGGCGGTTGTGGCGAAACCATTCGGAACGGAAACACCCATTCCGGa                                        <  1:1113689/140‑1 (MQ=255)                                                                                         cAAGCTGAGTAACATCGTCAATATCCGTTTTATCCAGCAGTCCATAAATGCGCTGGTTTACGCCGCTTTGGTCCAGAAACTGGTTAAACGCGTCGGCGGTTGTGGCGAAACCATTCGGAACGGAAACACCCATTCCGGaa                                       <  2:937573/140‑1 (MQ=255)                                                                                          aaGCTGAGTAACATCGTCAATATCCGTTTTATCCAGCAGTCCATAAATGCGCTGGTTTACGCCGCTTTGGTCCAGAAACTGGTTAAACGCGTCGGCGGTTGTGGCGAAACCATTCGGAACGGAAACACCCATTCCGGaaa                                      <  2:717622/140‑1 (MQ=255)                                                                                              tGAGTAACATCGTCAATATCCGTTTTATCCAGCAGTCCATAAATGCGCTGGTTTACGCCGCTTTGGTCCAGAAACTGGTTAAACGCGTCGGCGGTTGTGGCGAAACCATTCGGAACGGAAACACCCATTCCGGAAAGAtt                                  >  2:402032/1‑140 (MQ=255)                                                                                              tGAGTAACATCGTCAATATCCGTTTTATCCAGCAGTCCATAAATGCGCTGGTTTACGCCGCTTTGGTCCAGAAACTGGTTAAACGCGTCGGCGGTTGTGGCGAAACCATTCGGAACGGAAACACCCATTCCGGAAAGAtt                                  >  1:1336348/1‑140 (MQ=255)                                                                                               gAGTAACATCGTCAATATCCGTTTTATCCAGCAGTCCATAAATGCGCTGGTTTACGCCGCTTTGGTCCAGAAACTGGTTAAACGCGTCGGCGGTTGTGGCGAAACCATTCGGAACGGAAACACCCATTCCGGAAAGATTa                                 <  2:894101/140‑1 (MQ=255)                                                                                                aGTAACATCGTCAATATCCGTTTTATCCAGCAGTCCATAAATGCGCTGGTTTACGCCGCTTTGGTCCAGAAACTGGTTAAACGCGTCGGCGGTTGTGGCGAAACCATTCGGAACGGAAACACCCATTCCGGAAAGATTAg                                >  1:921101/1‑140 (MQ=255)                                                                                                   aaCATCGTCAATATCCGTTTTATCCAGCAGTCCATAAATGCGCTGGTTTACGCCGCTTTGGTCCAGAAACTGGTTAAACGCGTCGGCGGTTGTGGCGAAACCATTCGGAACGGAAACACCCATTCCGGAAAGATTAGTaa                             <  2:338470/140‑1 (MQ=255)                                                                                                        cGTCAATATCCGTTTTATCCAGCAGTCCATAAATGCGCTGGTTTACGCCGCTTTGGTCCAGAAACTGGTTAAACGCGTCGGCGGTTGTGGCGAAACCATTCGGAACGGAAACACCCATTCCGGAAAGATTAGTaa                             >  1:1258573/1‑135 (MQ=255)                                                                                                        cGTCAATATCCGTTTTATCCAGCAGTCCATAAATGCGCTGGTTTACGCCGCTTTGGTCCAGAAACTGGTTAAACGCGTCGGCGGTTGTGGCGAAACCATTCGGAACGGAAACACCCATTCCGGAAAGATTAGTaa                             <  2:1258573/135‑1 (MQ=255)                                                                                                          tCAATATCCGTTTTATCCAGCAGTCCATAAATGCGCTGGTTTACGCCGCTTTGGTCCAGAAACTGGTTAAACGCGTCGGCGGTTGTGGCGAAACCATTCGGAACGGAAACACCCATTCCGGAAAGATTAGTAATc                           <  1:166060/135‑1 (MQ=255)                                                                                                          tCAATATCCGTTTTATCCAGCAGTCCATAAATGCGCTGGTTTACGCCGCTTTGGTCCAGAAACTGGTTAAACGCGTCGGCGGTTGTGGCGAAACCATTCGGAACGGAAACACCCATTCCGGAAAGATTAGTAATc                           >  2:166060/1‑135 (MQ=255)                                                                                                          tCAATATCCGTTTTATCCAGCAGTCCATAAATGCGCTGGTTTACGCCGCTTTGGTCCAGAAACTGGTTAAACGCGTCGGCGGTTGTGGCGAAACCATTCGGAACGGAAACACCCATTCCGGAAAGATTAGTAATCATTTc                      >  2:27053/1‑140 (MQ=255)                                                                                                            aaTATCCGTTTTATCCAGCAGTCCATAAATGCGCTGGTTTACGCCGCTTTGGTCCAGAAACTGGTTAAACGCGTCGGCGGTTGGGGCGGAAACATTTGGAACGGAAACACCCCTTCCGGAAAGAATAATAAACATTTCAc                    >  2:1122329/1‑140 (MQ=255)                                                                                                                    ttttATCCAGCAGTCCATAAATGCGCTGGTTTACGCCGCTTTGGTCCAGAAACTGGTTAAACGCGTCGGCGGTTGTGGCGAAACCATTCGGAACGGAAACACCCATTCCGGAAAGATTAGTAATCATTTCACCCAGGGAg            >  1:527875/1‑140 (MQ=255)                                                                                                                    ttttATCCAGCAGTCCATAAATGCGCTGGTTTACGCCGCTTTGGTCCAGAAACTGGTTAAACGCGTCGGCGGTTGTGGCGAAACCATTCGGAACGGAAACACCCATTCCGGAAAGATTAGTAATCATTTCACCCAGGGAg            >  1:451455/1‑140 (MQ=255)                                                                                                                      ttATCCAGCAGTCCATAAATGCGCTGGTTTACGCCGCTTTGGTCCAGAAACTGGTTAAACGCGTCGGCGGTTGTGGCGAAACCATTCGGAACGGAAACACCCATTCCGGAAAGATTAGTAATCATTTCACCCAGGGAGGc          >  2:847339/1‑140 (MQ=255)                                                                                                                              cagTCCATAAATGCGCTGGTTTACGCCGCTTTGGTCCAGAAACTGGTTAAACGCGTCGGCGGTTGTGGCGAAACCATTCGGAACGGAAACACCCATTCCGGAAAGATTAGTAATCATTTCACCCAGGGAGGCATTTTTGc  >  2:599385/1‑140 (MQ=255)                                                                                                                                  |                                                                                                                                        TGTGCATAGGCTTCGCGGATGGCGTTTTCCAGCTCAGGCTGGAAGGGAGTGTCGATAATCCACTGGCGGATTTGCGCGCCCGCTTTCGCAAGCTGAGTAACATCGTCAATATCCGTTTTATCCAGCAGTTCATAAATGCGCTGGTTTACGCCGCTTTGGTCCAGAAACTGGTTAAACGCGTCGGCGGTTGTGGCGAAACCATTCGGAACGGAAACACCCATTCCGGAAAGATTAGTAATCATTTCACCCAGGGAGGCATTTTTGC  >  NC_000913/1786772‑1787036

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑