Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,384,176	G→A	L20L (CTG→CTA)	ycjX →	DUF463 domain‑containing protein YcjX

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,384,176	0	G	A	100.0%	204.8 / NA	58	L20L (CTG→CTA)	ycjX	DUF463 domain‑containing protein YcjX
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (31/27);  total (31/27)

ATCAATGGCCTGGCCCCCACATTCATATCCTTACGAATGATTTTTTTTCTGGTCTTCAGAGCAGCACAGGACAGCAATGAAGCGACTTAAAAATGAACTCAATGCGCTGGTGAATCGGGGTGTCGACAGACATCTGCGCCTCGCTGTAACCGGACTTAGCCGCAGCGGCAAAACAGCGTTTATCACCGCGATGGTTAATCAGTTGCTTAATATTCACGCCGGAGCACGTTTGCCGCTGTTAAGTGCGGTGCGTGAAGAGCGCCTGCTG  >  NC_000913/1384041‑1384308                                                                                                                                        |                                                                                                                                     aTCAATGGCCTGGCCCCCACATTCATATCCTTACGAATGATTTTTTTTCTGGTCTTCAGAGCAGCACAGGACAGCAATGAAGCGACTTAAAAATGAACTCAATGCGCTGGTGAATCGGGGTGTCGACAGACATCTACGcc                                                                                                                                  <  2:770375/140‑1 (MQ=255)  tCAATGGCCTGGCCCCCACATTCATATCCTTACGAATGATTTTTTTTCTGGTCTTCAGAGCAGCACAGGACAGCAATGAAGCGACTTAAAAATGAACTCAATGCGCTGGTGAATCGGGGTGTCGACAGACATCTACGCCt                                                                                                                                 <  1:590046/140‑1 (MQ=255)     aTGGCCTGGCCCCCACATTCATATCCTTACGAATGATTTTTTTTCTGGTCTTCAGAGCAGCACAGGACAGCAATGAAGCGACTTAAAAATGAACTCAATGCGCTGGTGAATCGGGGTGTCGACAGACATCTACGCCTCGc                                                                                                                              >  2:874343/1‑140 (MQ=255)       ggcctggccCCCACATTCATATCCTTACGAATGATTTTTTTTCTGGTCTTCAGAGCAGCACAGGACAGCAATGAAGCGACTTAAAAATGAACTCAATGCGCTGGTGAATCGGGGTGTCGACAGACATCTACGCCTCGCTg                                                                                                                            >  2:663291/1‑140 (MQ=255)          ctggccCCCACATTCATACCCTTACGAATGATTTTTTTTCTGGTCTTCAGAGCAGCACAGGACAGCAATGAAGCGACTAAAAAATGAACTCAATGCGCTGGTGAATCGGGGTGTCGACAGACATCTACGCCTCGCTGTaa                                                                                                                         <  2:286992/140‑1 (MQ=255)           tggccCCCACATTCATATCCTTACGAATGATTTTTTTTCTGGTCTTCAGAGCAGCACAGGACAGCAATGAAGCGACTTAAAAATGAACTCAATGCGCTGGTGAATCGGGGTGTCGACAGACATCTACGCCTCGCTGTAAc                                                                                                                        <  2:282977/140‑1 (MQ=255)              ccccaACATTCATATCCTTACGAATGATTTTTTTTCTGGTCTTCAGAGCAGCACAGGACAGCAATGAAGCGACTTAAAAATGAACTCAATGCGCTGGTGAATCGGGGTGTCGACAGACATCTACGCCTCGCTGTAACCgg                                                                                                                     >  2:1027605/1‑140 (MQ=255)                  cacaTTCATATCCTTACGAATGATTTTTTTTCTGGTCTTCAGAGCAGCACAGGACAGCAATGAAGCGACTTAAAAATGAACTCAATGCGCTGGTGAATCGGGGTGTCGACAGACATCTACGCCTCGCTGTAACCGGACtt                                                                                                                 >  1:97719/1‑140 (MQ=255)                       tCATATCCTTACGAATGATTTTTTTTCTGGTCTTCAGAGCAGCACAGGACAGCAATGAAGCGACTTAAAAATGAACTCAATGCGCTGGTGAATCGGGGTGTCGACAGACATCTACGCCTCGCTGTAACCGGACTTAGCCg                                                                                                            >  2:423596/1‑140 (MQ=255)                          tatCCTTACGAATGATTTTTTTTCTGGTCTTCAGAGCAGCACAGGACAGCAATGAAGCGACTTAAAAATGAACTCAATGCGCTGGTGAATCGGGGTGTCGACAGACATCTACGCCTCGCTGTAACCGGACTTAGCCGCAg                                                                                                         <  2:839095/140‑1 (MQ=255)                               ttACGAATGATTTTTTTTCTGGTCTTCAGAGCAGCACAGGACAGCAATGAAGCGACTTAAAAATGAACTCAATGCGCTGGTGAATCGGGGTGTCGACAGACATCTACGCCTCGCTGTAACCGGACTTAGCCGCAGCGGCa                                                                                                    >  1:1249019/1‑140 (MQ=255)                               ttACGAATGATTTTTTTTCTGGTCTTCAGAGCAGCACAGGACAGCAATGAAGCGACTTAAAAATGAACTCAATGCGCTGGTGAATCGGGGTGTCGACAGACATCTACGCCTCGCTGTAACCGGACTTAGCCGCAGCGGCa                                                                                                    >  2:1121599/1‑140 (MQ=255)                               ttACGAATGATTTTTTTTCTGGTCTTCAGAGCAGCACAGGACAGCAATGAAGCGACTTAAAAATGAACTCAATGCGCTGGTGAATCGGGGTGTCGACAGACATCTACGCCTCGCTGTAACCGGACTTAGCCGCAGCGGCa                                                                                                    >  2:1013674/1‑140 (MQ=255)                                 aCGAATGATTTTTTTTCTGGTCTTCAGAGCAGCACAGGACAGCAATGAAGCGACTTAAAAATGAACTCAATGCGCTGGTGAATCGGGGTGTCGACAGACATCTACGCCTCGCTGTAACCGGACTTAGCCGCAGCGGCaaa                                                                                                  >  2:1508396/1‑140 (MQ=255)                                 aCGAATGATTTTTTTTCTGGTCTTCAGAGCAGCACAGGACAGCAATGAAGCGACTTAAAAATGAACTCAATGCGCTGGTGAATCGGGGTGTCGACAGACATCTACGCCTCGCTGTAACCGGACTTAGCCGCAGCGGCaaa                                                                                                  >  1:432855/1‑140 (MQ=255)                                  cGAATGATTTTTTTTCTGGTCTTCAGAGCAGCACAGGACAGCAATGAAGCGACTTAAAAATGAACTCAATGCGCTGGTGAATCGGGGTGTCGACAGACATCTACGCCTCGCTGTAACCGGACTTAGCCGCAGCGGCaaaa                                                                                                 <  2:1105667/140‑1 (MQ=255)                                    aaTGATTTTTTTTCTGGTCTTCAGAGCAGCACAGGACAGCAATGAAGCGACTTAAAAATGAACTCAATGCGCTGGTGAATCGGGGTGTCGACAGACATCTACGCCTCGCTGTAACCGGACTTAGCCGCAGCGGCAAAACa                                                                                               <  1:465929/140‑1 (MQ=255)                                       gATTTTTTTTCTGGTCTTCAGAGCAGCACAGGACAGCAATGAAGCGACTTAAAAATGAACTCAATGCGCTGGTGAATCGGGGTGTCGACAGACATCTACGCCTCGCTGTAACCGGACTTAGCCGCAGCGGCAAAACAGCg                                                                                            >  2:35695/1‑140 (MQ=255)                                       gATTTTTTTTCTGGTCTTCAGAGCAGCACAGGACAGCAATGAAGCGACTTAAAAATGAACTCAATGCGCTGGTGAATCGGGGTGTCGACAGACATCTACGCCTCGCTGTAACCGGACTTAGCCGCAGCGGCAAAACAGCg                                                                                            >  2:391548/1‑140 (MQ=255)                                        aTTTTTTTTCTGGTCTTCAGAGCAGCACAGGACAGCAATGAAGCGACTTAAAAATGAACTCAATGCGCTGGTGAATCGGGGTGTCGACAGACATCTACGCCTCGCTGTAACCGGACTTAGCCGCAGCGGCAAAACAGCGt                                                                                           >  1:434162/1‑140 (MQ=255)                                                  tGGTCTTCAGAGCAGCACAGGACAGCAATGAAGCGACTTAAAAATGAACTCAATGCGCTGGTGAATCGGGGTGTCGACAGACATCTACGCCTCGCTGTAACCGGACTTAGCCGCAGCGGCAAAACAGCGTTTATCACcgc                                                                                 <  2:97719/140‑1 (MQ=255)                                                    gTCTTCAGAGCAGCACAGGACAGCAATGAAGCGACTTAAAAATGAACTCAATGCGCTGGTGAATCGGGGTGTCGACAGACATCTACGCCTCGCTGTAACCGGACTTAGCCGCAGCGGCAAAACAGCGTTTATCACCGCGa                                                                               <  2:1442627/140‑1 (MQ=255)                                                     tCTTCAGAGCAGCACAGGACAGCAATGAAGCGACTTAAAAATGAACTCAATGCGCTGGTGAATCGGGGTGTCGACAGACATCTACGCCTCGCTGTAACCGGACTTAGCCGCAGCGGCAAAACAGCGTTTATCACCGCGAt                                                                              <  1:1508396/140‑1 (MQ=255)                                                        tCAGAGCAGCACAGGACAGCAATGAAGCGACTTAAAAATGAACTCAATGCGCTGGTGAATCGGGGTGTCGACAGACATCTACGCCTCGCTGTAACCGGACTTAGCCGCAGCGGCAAAACAGCGTTTATCACCGCGATGGt                                                                           <  2:996743/140‑1 (MQ=255)                                                            agcagcACAGGACAGCAATGAAGCGACTTAAAAATGAACTCAATGCGCTGGTGAATCGGGGTGTCGACAGACATCTACGCCTCGCTGTAACCGGACTTAGCCGCAGCGGCAAAACAGCGTTTATCACCGCGATGGTTAAt                                                                       <  2:439767/140‑1 (MQ=255)                                                             gcagcaCAGGACAGCAATGAAGCGACTTAAAAATGAACTCAATGCGCTGGTGAATCGGGGTGTCGACAGACATCTACGCCTCGCTGTAACCGGACTTAGCCGCAGCGGCAAAACAGCGTTTATCACCGCGATGGTTAATc                                                                      <  2:434162/140‑1 (MQ=255)                                                               agcaCAGGACAGCAATGAAGCGACTTAAAAATGAACTCAATGCGCTGGTGAATCGGGGTGTCGACAGACATCTACGCCTCGCTGTAACCGGACTTAGCCGCAGCGGCAAAACAGCGTTTATCACCGCGATGGTTAATCAg                                                                    >  2:291774/1‑140 (MQ=255)                                                                 cacaGGACAGCAATGAAGCGACTTAAAAATGAACTCAATGCGCTGGTGAATCGGGGTGTCGACAGACATCTACGCCTCGCTGTAACCGGACTTAGCCGCAGCGGCAAAACAGCGTTTATCACCGCGAt                                                                              <  2:981637/128‑1 (MQ=255)                                                                 cacaGGACAGCAATGAAGCGACTTAAAAATGAACTCAATGCGCTGGTGAATCGGGGTGTCGACAGACATCTACGCCTCGCTGTAACCGGACTTAGCCGCAGCGGCAAAACAGCGTTTATCACCGCGAt                                                                              >  1:981637/1‑128 (MQ=255)                                                                 cacaGGACAGCAATGAAGCGACTTAAAAATGAACTCAATGCGCTGGTGAATCGGGGTGTCGACAGACATCTACGCCTCGCTGTAACCGGACTTAGCCGCAGCGGCAAAACAGCGTTTATCACCGCGATGGTTa                                                                         >  2:942679/1‑133 (MQ=255)                                                                 cacaGGACAGCAATGAAGCGACTTAAAAATGAACTCAATGCGCTGGTGAATCGGGGTGTCGACAGACATCTACGCCTCGCTGTAACCGGACTTAGCCGCAGCGGCAAAACAGCGTTTATCACCGCGATGGTTa                                                                         <  1:942679/133‑1 (MQ=255)                                                                 cacaGGACAGCAATGAAGCGACTTAAAAATGAACTCAATGCGCTGGTGAATCGGGGTGTCGACAGACATCTACGCCTCGCTGTAACCGGACTTAGCCGCAGCGGCAAAACAGCGTTTATCACCGCGATGGTTAATCAGtt                                                                  <  2:513776/140‑1 (MQ=255)                                                                 cacaGGACAGCAATGAAGCGACTTAAAAATGAACTCAATGCGCTGGTGAATCGGGGTGTCGACAGACATCTACGCCTCGCTGTAACCGGACTTAGCCGCAGCGGCAAAACAGCGTTTATCACCGCGATGGTTAATCAGtt                                                                  >  1:1324096/1‑140 (MQ=255)                                                                   caGGACAGCAATGAAGCGACTTAAAAATGAACTCAATGCGCTGGTGAATCGGGGTGTCGACAGACATCTACGCCTCGCTGTAACCGGACTTAGCCGCAGCGGCAAAACAGCGTTTATCACCGCGATGGTTAATCAGTTGc                                                                >  2:892771/1‑140 (MQ=255)                                                                      gACAGCAATGAAGCGACTTAAAAATGAACTCAATGCGCTGGTGAATCGGGGTGTCGACAGACATCTACGCCTCGCTGTAACCGGACTTAGCCGCAGCGGCAAAACAGCGTTTATCACCGCGATGGTTAATCAGTTGCTTa                                                             >  1:1012031/1‑140 (MQ=255)                                                                      gACAGCAATGAAGCGACTTAAAAATGAACTCAATGCGCTGGTGAATCGGGGTGTCGACAGACATCTACGCCTCGCTGTAACCGGACTTAGCCGCAGCGGCAAAACAGCGTTTATCACCGCGATGGTTAATCAGTTGCTTa                                                             >  1:318446/1‑140 (MQ=255)                                                                      gACAGCAATGAAGCGACTTAAAAATGAACTCAATGCGCTGGTGAATCGGGGTGTCGACAGACATCTACGCCTCGCTGTAACCGGACTTAGCCGCAGCGGCAAAACAGCGTTTATCACCGCGATGGTTAATCAGTTGCTTa                                                             >  1:183609/1‑140 (MQ=255)                                                                           cAATGAAGCGACTTAAAAATGAACTCAATGCGCTGGTGAATCGGGGTGTCGACAGACATCTACGCCTCGCTGTAACCGGACTTAGCCGCAGCGGCAAAACAGCGTTTATCACCGCGATGGTTAATCAGTTGCTTAATAtt                                                        >  1:876915/1‑140 (MQ=255)                                                                           cAATGAAGCGACTTAAAAATGAACTCAATGCGCTGGTGAATCGGGGTGTCGACAGACATCTACGCCTCGCTGTAACCGGACTTAGCCGCAGCGGCAAAACAGCGTTTATCACCGCGATGGTTAATCAGTTGCTTAATAtt                                                        >  2:418201/1‑140 (MQ=255)                                                                           cAATGAAGCGACTTAAAAATGAACTCAATGCGCTGGTGAATCGGGGCGTCGACAGACATCTACGCCTCGCTGTAACCGGACTTAGCCGCAGCGGCAAAACAGCGTTTATCACCGCGATGGTTAATCAGTTGCTTAATAtt                                                        >  1:557582/1‑140 (MQ=255)                                                                                aaGCGACTTAAAAATGAACTCAATGCGCTGGTGAATCGGGGTGTCGACAGACATCTACGCCTCGCTGTAACCGGACTTAGCCGCAGCGGCAAAACAGCGTTTATCACCGCGATGGTTAATCAGTTGCTTAATATTCACGc                                                   <  2:876915/140‑1 (MQ=255)                                                                                   cGACTTAAAAATGAACTCAATGCGCTGGTGAATCGGGGTGTCGACAGACATCTACGCCTCGCTGTAACCGGACTTAGCCGCAGCGGCAAAACAGCGTTTATCACCGCGATGGTTAATCAGTTGCTTAATATTCACGCCgg                                                >  2:429306/1‑140 (MQ=255)                                                                                                aaCTCAATGCGCTGGTGAATCGGGGTGTCGACAGACATCTACGCCTCGCTGTAACCGGACTTAGCCGCAGCGGCAAAACAGCGTTTATCACCGCGATGGTTAATCAGTTGCTTAATATTCACGCCGGAGCACGTTTGCCg                                   >  2:784110/1‑140 (MQ=255)                                                                                                     aaTGCGCTGGTGAATCGGGGTGTCGACAGACATCTACGCCTCGCTGTAACCGGACTTAGCCGCAGCGGCAAAACAGCGTTTATCACCGCGATGGTTAATCAGTTGCTTAATATTCACGCCGGAGCACGTTTGCCGCTGtt                              >  1:1424139/1‑140 (MQ=255)                                                                                                     aaTGCGCTGGTGAATCGGGGTGTCGACAGACATCTACGCCTCGCTGTAACCGGACTTAGCCGCAGCGGCAAAACAGCGTTTATCACCGCGATGGTTAATCAGTTGCTTAATATTCACGCCGGAGCACGTTTGCCGCTGtt                              <  2:1324096/140‑1 (MQ=255)                                                                                                           cTGGTGAATCGGGGTGTCGACAGACATCTACGCCTCGCTGTAACCGGACTTAGCCGCAGCGGCAAAACAGCGTTTATCACCGCGATGGTTAATCAGTTGCTTAATATTCACGCCGGAGCACGTTTGCCGCTGTTAAgtgc                        <  1:291774/140‑1 (MQ=255)                                                                                                           cTGGTGAATCGGGGTGTCGACAGACATCTACGCCTCGCTGTAACCGGACTTAGCCGCAGCGGCAAAACAGCGTTTATCACCGCGATGGTTAATCAGTTGCTTAATATTCACGCCGGAGCACGTTTGCCGCTGTTAAgtgc                        <  1:418201/140‑1 (MQ=255)                                                                                                           cTGGTGAATCGGGGTGTCGACAGACATCTACGCCTCGCTGTAACCGGACTTAGCCGCAGCGGCAAAACAGCGTTTATCACCGCGATGGTTAATCAGTTGCTTAATATTCACGCCGGAGCACGTTTGCCGCTGTTAAgtgc                        <  1:663291/140‑1 (MQ=255)                                                                                                             ggTGAATCGGGGTGTCGACAGACATCTACGCCTCGCTGTAACCGGACTTAGCCGCAGCGGCAAAACAGCGTTTATCACCGCGATGGTTAATCAGTTGCTTAATATTCACGCCGGAGCACGTTTGCCGCTGTTAAgtgcgg                      <  1:638360/140‑1 (MQ=255)                                                                                                             ggTGAATCGGGGTGTCGACAGACATCTACGCCTCGCTGTAACCGGACTTAGCCGCAGCGGCAAAACAGCGTTTATCACCGCGATGGTTAATCAGTTGCTTAATATTCACGCCGGAGCACGTTTGCCGCTGTTAAgtgcgg                      <  1:784110/140‑1 (MQ=255)                                                                                                                    cGGGGTGTCGACAGACATCTACGCCTCGCTGTAACCGGACTTAGCCGCAGCGGCAAAACAGCGTTTATCACCGCGATGGTTAATCAGTTGCTTAATATTCACGCCGGAGCACGTTTGCCGCTGTTAAGTGCGGTGCGTGa               <  2:183609/140‑1 (MQ=255)                                                                                                                    cGGGGTGTCGACAGACATCTACGCCTCGCTGTAACCGGACTTAGCCGCAGCGGCAAAACAGCGTTTATCACCGCGATGGTTAATCAGTTGCTTAATATTCACGCCGGAGCACGTTTGCCGCTGTTAAGTGCGGTGCGTGa               <  2:1012031/140‑1 (MQ=255)                                                                                                                      gggTGTCGACAGACATCTACGCCTCGCTGTAACCGGACTTAGCCGCAGCGGCAAAACAGCGTTTATCACCGCGATGGTTAATCAGTTGCTTAATATTCACGCCGGAGCACGTTTGCCGCTGTTAAGTGCGGTGCGTGAag             <  1:423596/140‑1 (MQ=255)                                                                                                                        gtgtCGACAGACATCTACGCCTCGCTGTAACCGGACTTAGCCGCAGCGGCAAAACAGCGTTTATCACCGCGATGGTTAATCAGTTGCTTAATATTCACGCCGGAGCACGTTTGCCGCTGTTAAGTGCGGTGCGTGAagag           <  2:1249019/140‑1 (MQ=255)                                                                                                                        gtgtCGACAGACATCTACGCCTCGCTGTAACCGGACTTAGCCGCAGCGGCAAAACAGCGTTTATCACCGCGATGGTTAATCAGTTGCTTAATATTCACGCCGGAGCACGTTTGCCGCTGTTAAGTGCGGTGCGTGAagag           >  1:686014/1‑140 (MQ=255)                                                                                                                           tCGACAGACATCTACGCCTCGCTGTAACCGGACTTAGCCGCAGCGGCAAAACAGCGTTTATCACCGCGATGGTTAATCAGTTGCTTAATATTCACGCCGGAGCACGTTTGCCGCTGTTAAGTGCGGTGCGTGAAGAgcgc        >  1:297557/1‑140 (MQ=255)                                                                                                                           tCGACAGACATCTACGCCTCGCTGTAACCGGACTTAGCCGCAGCGGCAAAACAGCGTTTATCACCGCGATGGTTAATCAGTTGCTTAATATTCACGCCGGAGCACGTTTGCCGCTGTTAAGTGCGGTGCGTGAAGAgcgc        >  1:866601/1‑140 (MQ=255)                                                                                                                                 gacaTCTACGCCTCGCTGTAACCGGACTTAGCCGCAGCGGCAAAACAGCGTTTATCACCGCGATGGTTAATCAGTTGCTTAATATTCACGCCGGAGCACGTTTGCCGCTGTTAAGTGCGGTGCGTGAAGAGCGCctgctg  >  1:452562/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                     ATCAATGGCCTGGCCCCCACATTCATATCCTTACGAATGATTTTTTTTCTGGTCTTCAGAGCAGCACAGGACAGCAATGAAGCGACTTAAAAATGAACTCAATGCGCTGGTGAATCGGGGTGTCGACAGACATCTGCGCCTCGCTGTAACCGGACTTAGCCGCAGCGGCAAAACAGCGTTTATCACCGCGATGGTTAATCAGTTGCTTAATATTCACGCCGGAGCACGTTTGCCGCTGTTAAGTGCGGTGCGTGAAGAGCGCCTGCTG  >  NC_000913/1384041‑1384308

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑