Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,721,915	Δ1 bp	coding (652/858 nt)	ydhJ →	putative membrane fusion protein YdhJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,721,912	0	A	.	100.0%	184.0 / NA	43	coding (649/858 nt)	ydhJ	putative membrane fusion protein YdhJ
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (22/21);  total (22/21)

GATTACGCTGATACCGGGAAACCGCTGTTTGCCCTTGTCGACAGCCACTCGTTTTACGTCATTGGTTATTTTGAAGAAACCAAGTTGCGCCATATCCGCGAAGGTGCACCAGCACAAATTACCCTCTATAGTGATAACAAAACGTTACAGGGTCACGTTTCCAGTATCGGTCGGGCGATTTATGATCAGAGCGTTGAAAGTGACTCAAGCCTTATTCCGGATGTTAAACCTAACGTCCCCTGGGTTCGACTCGCCCAACGCGTTCCCGTC  >  NC_000913/1721774‑1722043                                                                                                                                           |                                                                                                                                    gATTACGCTGATACCGGGAAACCGCTGTTTGCCCTTGTCGACAGCCACTCGTTTTACGTCATTGGTTATTTTGAAGAAACCAAGTTGCGCCATATCCGCGAAGGTGCACCAGCACAAATTACCCTCTATAGTGATAACaa                                                                                                                                    <  1:1146512/140‑1 (MQ=255)  aTTACGCTGATACCGGGAAACCGCTGTTTGCCCTTGTCGATAGCCACTCGTTTTACGTCATTGGTTATTTTGAAGAAACCAAGTTGCGCCATATCCGCGAAGGTGCACCAGCACAAATTACCCTCTATAGTGATAACaaa                                                                                                                                   <  1:904890/140‑1 (MQ=255)          gATACCGGGAAACCGCTGTTTGCCCTTGTCGACAGCCACTCGTTTTACGTCATTGGTTATTTTGAAGAAACCAAGTTGCGCCATATCCGCGAAGGTGCACCAGCACAAATTACCCTCTATAGTGATAACa                                                                                                                                     <  1:1008694/130‑1 (MQ=255)          gATACCGGGAAACCGCTGTTTGCCCTTGTCGACAGCCACTCGTTTTACGTCATTGGTTATTTTGAAGAAACCAAGTTGCGCCATATCCGCGAAGGTGCACCAGCACAAATTACCCTCTATAGTGATAACa                                                                                                                                     >  2:1008694/1‑130 (MQ=255)             aCCGGGAAACCGCTGTTTGCCCTTGTCGACAGCCACTCGTTTTACGTCATTGGTTATTTTGAAGAAACCAAGTTGCGCCATATCCGCGAAGGTGCACCAGCACAAATTACCCTCTATAGTGATAAC‑AAACGTTACAGGGt                                                                                                                       <  1:191058/140‑1 (MQ=255)             aCCGGGAAACCGCTGTTTGCCCTTGTCGACAGCCACTCGTTTTACGTCATTGGTTATTTTGAAGAAACCAAGTTGCGCCATATCCGCGAAGGTGCACCAGCACAAATTACCCTCTATAGTGATAAC‑AAACGTTACAGGGt                                                                                                                       <  1:383198/140‑1 (MQ=255)                gggAAACCGCTGTTTGCCCTTGTCGACAGCCACTCGTTTTACGTCATTGGTTATTTTGAAGAAACCAAGTTGCGCCATATCCGCGAAGGTGCACCAGCACAAATTACCCTCTATAGTGATAAC‑AAACGTTACAGGGTCAc                                                                                                                    <  2:1201484/140‑1 (MQ=255)                gggAAACCGCTGTTTGCCCTTGTCGACAGCCACTCGTTTTACGTCATTGGTTATTTTGAAGAAACCAAGTTGCGCCATATCCGCGAAGGTGCACCAGCACAAATTACCCTCTATAGTGATAAC‑AAACGTTACAGGGTCAc                                                                                                                    >  1:810410/1‑140 (MQ=255)                gggAAACCGCTGTTTGCCCTTGTCGACAGCCACTCGTTTTACGTCATTGGTTATTTTGAAGAAACCAAGTTGCGCCATATCCGCGAAGGTGCACCAGCACAAATTACCCTCTATAGTGATAAC‑AAACGTTACAGGGTCAc                                                                                                                    <  1:360598/140‑1 (MQ=255)                 ggAAACCGCTGTTTGCCCTTGTCGACAGCCACTCGTTTTACGTCATTGGTTATTTTGAAGAAACCAAGTTGCGCCATATCCGCGAAGGTGCACCAGCACAAATTACCCTCTATAGTGATAACa                                                                                                                                     >  2:112398/1‑123 (MQ=255)                 ggAAACCGCTGTTTGCCCTTGTCGACAGCCACTCGTTTTACGTCATTGGTTATTTTGAAGAAACCAAGTTGCGCCATATCCGCGAAGGTGCACCAGCACAAATTACCCTCTATAGTGATAACa                                                                                                                                     <  1:112398/123‑1 (MQ=255)                   aaaCCGCTGTTTGCCCTTGTCGACAGCCACTCGTTTTACGTCATTGGTTATTTTGAAGAAACCAAGTTGCGCCATATCCGCGAAGGTGCACCAGCACAAATTACCCTCTATAGTGATAAC‑AAACGTTACAGGGTCACGtt                                                                                                                 <  1:259181/140‑1 (MQ=255)                       cGCTGTTTGCCCTTGTCGACAGCCACTCGTTTTACGTCATTGGTTATTTTGAAGAAACCAAGTTGCGCCATATCCGCGAAGGTGCACCAGCACAAATTACCCTCTATAGTGATAAC‑AAACGTTACAGGGTCACGTTTCCa                                                                                                             >  1:956084/1‑140 (MQ=255)                           gTTTGCCCTTGTCGACAGCCACTCGTTTTACGTCATTGGTTATTTTGAAGAAACCAAGTTGCGCCATATCCGCGAAGGTGCACCAGCACAAATTACCCTCTATAGTGATAAC‑AAACGTTACAGGGTCACGTTTCCAGTAt                                                                                                         <  2:956084/140‑1 (MQ=255)                              tGCCCTTGTCGACAGCCACTCGTTTTACGTCATTGGTTATTTTGAAGAAACCAAGTTGCGCCATATCCGCGAAGGTGCACCAGCACAAATTACCCTCTATAGTGATAAC‑AAACGTTACAGGGTCACGTTTCCAGTAtcgg                                                                                                      >  2:81747/1‑140 (MQ=255)                                  cTTGTCGACAGCCACTCGTTTTACGTCATTGGTTATTTTGAAGAAACCAAGTTGCGCCATATCCGCGAAGGTGCACCAGCACAAATTACCCTCTATAGTGATAAC‑AAACGTTACAGGGTCACGTTTCCAGTAtcggtcgg                                                                                                  <  2:810410/140‑1 (MQ=255)                                    tGTCGACAGCCACTCGTTTTACGTCATTGGTTATTTTGAAGAAACCAAGTTGCGCCATATCCGCGAAGGTGCACCAGCACAAATTACCCTCTATAGTGATAAC‑AAACGTTACAGGGTCACGTTTCCAGTATCGGTCGGGc                                                                                                <  1:1035695/140‑1 (MQ=255)                                        gACAGCCACTCGTTTTACGTCATTGGTTATTTTGAAGAAACCAAGTTGCGCCATATCCGCGAAGGTGCACCAGCACAAATTACCCTCTATAGTGATAAC‑AAACGTTACAGGGTCACGTTTCCAGTATCGGTCGGGCGAtt                                                                                            <  2:741391/140‑1 (MQ=255)                                                cTCGTTTTACGTCATTGGTTATTTTGAAGAAACCAAGTTGCGCCATATCCGCGAAGGTGCACCAGCACAAATTACCCTCTATAGTGATAAC‑AAACGTTACAGGGTCACGTTTCCAGTATCGGTCGGGCGATTTATGATc                                                                                     >  2:1279889/1‑139 (MQ=255)                                                cTCGTTTTACGTCATTGGTTATTTTGAAGAAACCAAGTTGCGCCATATCCGCGAAGGTGCACCAGCACAAATTACCCTCTATAGTGATAAC‑AAACGTTACAGGGTCACGTTTCCAGTATCGGTCGGGCGATTTATGATc                                                                                     <  1:1279889/139‑1 (MQ=255)                                                  cGTTTTACGTCATTGGTTATTTTGAAGAAACCAAGTTGCGCCATATCCGCGAAGGTGCACCAGCACAAATTACCCTCTATAGTGATAAC‑AAACGTTACAGGGTCACGTTTCCAGTATCGGTCGGGCGATTTATGATCaga                                                                                  <  2:520223/140‑1 (MQ=255)                                                     tttACGTCATTGGTTATTTTGAAGAAACCAAGTTGCGCCATATCCGCGAAGGTGCACCAGCACAAATTACCCTCTATAGTGATAAC‑AAACGTTACAGGGTCACGTTTCCAGTATCGGTCGGGCGATTTATGAt                                                                                      <  1:339157/133‑1 (MQ=255)                                                     tttACGTCATTGGTTATTTTGAAGAAACCAAGTTGCGCCATATCCGCGAAGGTGCACCAGCACAAATTACCCTCTATAGTGATAAC‑AAACGTTACAGGGTCACGTTTCCAGTATCGGTCGGGCGATTTATGAt                                                                                      >  2:339157/1‑133 (MQ=255)                                                     tttACGTCATTGGTTATTTTGAAGAAACCAAGTTGCGCCATATCCGCGAAGGTGCACCAGCACAAATTACCCTCTATAGTGATAAC‑AAACGTTACAGGGTCACGTTTCCAGTATCGGTCGGGCGATTTATGATCAGAGCg                                                                               <  1:1003838/140‑1 (MQ=255)                                                     tttACGTCATTGGTTATTTTGAAGAAACCAAGTTGCGCCATATCCGCGAAGGTGCACCAGCACAAATTACCCTCTATAGTGATAAC‑AAACGTTACAGGGTCACGTTTCCAGTATCGGTCGGGCGATTTATGATCAGAGCg                                                                               >  2:1193372/1‑140 (MQ=255)                                                           tCATTGGTTATTTTGAAGAAACCAAGTTGCGCCATATCCGCGAAGGTGCACCAGCACAAATTACCCTCTATAGTGATAAC‑AAACGTTACAGGGTCACGTTTCCAGTATCGGTCGGGCGATTTATGATCAGAGCGTTGaaa                                                                         >  2:134751/1‑140 (MQ=255)                                                             aTTGGTTATTTTGAAGAAACCAAGTTGCGCCATATCCGCGAAGGTGCACCAGCACAAATTACCCTCTATAGTGATAAC‑AAACGTTACAGGGTCACGTTTCCAGTATCGGTCGGGCGATTTATGATCAGAGCGTTGAAAGt                                                                       >  2:338409/1‑140 (MQ=255)                                                              ttGGTTATTTTGAAGAAACCAAGTTGCGCCATATCCGCGAAGGTGCACCAGCACAAATTACCCTCTATAGTGATAAC‑AAACGTTACAGGGTCACGTTTCCAGTATCGGTCGGGCGATTTATGATCag                                                                                   <  2:101305/127‑1 (MQ=255)                                                              ttGGTTATTTTGAAGAAACCAAGTTGCGCCATATCCGCGAAGGTGCACCAGCACAAATTACCCTCTATAGTGATAAC‑AAACGTTACAGGGTCACGTTTCCAGTATCGGTCGGGCGATTTATGATCag                                                                                   >  1:101305/1‑127 (MQ=255)                                                               tGGTTATTTTGAAGAAACCAAGTTGCGCCATATCCGCGAAGGTGCACCAGCACAAATTACCCTCTATAGTGATAAC‑AAACGTTACAGGGTCACGTTTCCAGTATCGGTCGGGCGATTTATGATCAGAGCGTTGAAAGTGa                                                                     >  2:673484/1‑140 (MQ=255)                                                                  ttATTTTGAAGAAACCAAGTTGCGCCATATCCGCGAAGGTGCACCAGCACAAATTACCCTCTATAGTGATAAC‑AAACGTTACAGGGTCACGTTTCCAGTATCGGTCGGGCGATTTATGATCAGAGCGTTGAAAGTGACTc                                                                  <  1:338409/140‑1 (MQ=255)                                                                   tATTTTGAAGAAACCAAGTTGCGCCATATCCGCGAAGGTGCACCAGCACAAATTACCCTCTATAGTGATAAC‑AAACGTTACAGGGTCACGTTTCCAGTATCGGTCGGGCGATTTATGATCAGAGCGTTGAAAGTGACTCa                                                                 >  1:723377/1‑140 (MQ=255)                                                                               aCCAAGTTGCGCCATATCCGCGAAGGTGCACCAGCACAAATTACCCTCTATAGTGATAAC‑AAACGTTACAGGGTCACGTTTCCAGTATCGGTCGGGCGATTTATGATCAGAGCGTTGAAAGTGACTCAAGCCTTATTCCg                                                     <  2:725802/140‑1 (MQ=255)                                                                                   aGTTGCGCCATATCCGCGAAGGTGCACCAGCACAAATTACCCTCTATAGTGATAAC‑AAACGTTACAGGGTCACGTTTCCAGTATCGGTCGGGCGATTTATGATCAGAGCGTTGAAAGTGACTCAAGCCTTATTCCGGATg                                                 <  1:81747/140‑1 (MQ=255)                                                                                         gcCATATCCGCGAAGGTGCACCAGCACAAATTACCCTCTATAGTGATAAC‑AAACGTTACAGGGTCACGTTTCCAGTATCGGTCGGGCGATTTATGATCAGAGCGTTGAAAGTGACTCAAGCCTTATTCCGGATGTTAAAc                                           <  1:559386/140‑1 (MQ=255)                                                                                             tatCCGCGAAGGTGCACCAGCACAAATTACCCTCTATAGTGATAAC‑AAACGTTACAGGGTCACGTTTCCAGTATCGGTCGGGCGATTTATGATCAGAGCGTTGAAAGTGACTCAAGCCTTATTCCGGATGTTAAACCTaa                                       >  1:709770/1‑140 (MQ=255)                                                                                               tCCGCGAAGGTGCACCAGCACAAATTACCCTCTATAGTGATAAC‑AAACGTTACAGGGTCACGTTTCCAGTATCGGTCGGGCGATTTATGATCAGAGCGTTGAAAGTGACTCAAGCCTTATTCCGGATGTTAAACCTAACg                                     <  1:1222821/140‑1 (MQ=255)                                                                                                  gcgAAGGTGCACCAGCACAAATTACCCTCTATAGTGATAAC‑AAACGTTACAGGGTCACGTTTCCAGTATCGGTCGGGCGATTTATGATCAGAGCGTTGAAAGTGACTCAAGCCTTATTCCGGATGTTAAACCTAACGTcc                                  >  1:838553/1‑140 (MQ=255)                                                                                                   cgAAGGTGCACCAGCACAAATTACCCTCTATAGTGATAAC‑AAACGTTACAGGGTCACGTTTCCAGTATCGGTCGGGCGATTTATGATCAGAGCGTTGAAAGTGACTCAAGCCTTATTCCGGATGTTAAACCTAACGTccc                                 <  1:1193372/140‑1 (MQ=255)                                                                                                   cgAAGGTGCACCAGCACAAATTACCCTCTATAGTGATAAC‑AAACGTTACAGGGTCACGTTTCCAGTATCGGTCGGGCGATTTATGATCAGAGCGTTGAAAGTGACTCAAGCCTTATTCCGGATGTTAAACCTAACGTccc                                 <  2:675587/140‑1 (MQ=255)                                                                                                   cgAAGGTGCACCAGCACAAATTACCCTCTATAGTGATAAC‑AAACGTTACAGGGTCACGTTTCCAGTATCGGTCGGGCGATTTATGATCAGAGCGTTGAAAGTGACTCAAGCCTTATTCCGGATGTTAAACCTAACGTccc                                 >  1:409707/1‑140 (MQ=255)                                                                                                           cACCAGCACAAATTACCCTCTATAGTGATAAC‑AAACGTTACAGGGTCACGTTTCCAGTATCGGTCGGGCGATTTATGATCAGAGCGTTGAAAGTGACTCAAGCCTTATTCCGGATGTTAAACCTAACGTCCCCTGGGTTc                         >  2:641993/1‑140 (MQ=255)                                                                                                            aCCAGCACAAATTACCCTCTATAGTGATAAC‑AAACGTTACAGGGTCACGTTTCCAGTATCGGTCGGGCGATTTATGATCAGAGCGTTGAAAGTGACTCAAGCCTTATTCCGGATGTTAAACCTAACGTCCCCTGGGTTCg                        >  2:342581/1‑140 (MQ=255)                                                                                                                  acaAATTACCCTCTATAGTGATAAC‑AAACGTTACAGGGTCACGTTTCCAGTATCGGTCGGGCGATTTATGATCAGAGCGTTGAAAGTGACTCAAGCCTTATTCCGGATGTTAAACCTAACGTCCCCTGGGTTCGACTCGc                  >  1:1275951/1‑140 (MQ=255)                                                                                                                               tataGTGATAAC‑AAACGTTACAGGGTCACGTTTCCAGTATCGGTCGGGCGATTTATGATCAGAGCGTTGAAAGTGACTCAAGCCTTATTCCGGATGTTAAACCTAACGTCCCCTGGGTTCGACTCGCCCAACGCGTTccc     >  2:272496/1‑140 (MQ=255)                                                                                                                                ataGTGATAAC‑AAACGTTACAGGGTCACGTTTCCAGTATCGGTCGGGCGATTTATGATCAGAGCGTTGAAAGTGACTCAAGCCTTATTCCGGATGTTAAACCTAACGTCCCCTGGGTTCGACTCGCCCAACGCGTTCCCg    >  1:907598/1‑140 (MQ=255)                                                                                                                                ataGTGATAAC‑AAACGTTACAGGGTCACGTTTCCAGTATCGGTCGGGCGATTTATGATCAGAGCGTTGAAAGTGACTCAAGCCTTATTCCGGATGTTAAACCTAACGTCCCCTGGGTTCGACTCGCCCAACGCGTTCCCg    >  2:1275910/1‑140 (MQ=255)                                                                                                                                ataGTGATAAC‑AAACGTTACAGGGTCACGTTTCCAGTATCGGTCGGGCGATTTATGATCAGAGCGTTGAAAGTGACTCAAGCCTTATTCCGGATGTTAAACCTAACGTCCCCTGGGTTCGACTCGCCCAACGCGTTCCCg    >  1:295730/1‑140 (MQ=255)                                                                                                                                  aGTGATAAC‑AAACGTTACAGGGTCACGTTTCCAGTATCGGTCGGGCGATTTATGATCAGAGCGTTGAAAGTGACTCAAGCCTTATTCCGGATGTTAAACCTAACGTCCCCTGGGTTCGACTCGCCCAACGCGTTCCCGTc  >  1:196582/1‑140 (MQ=255)                                                                                                                                           |                                                                                                                                    GATTACGCTGATACCGGGAAACCGCTGTTTGCCCTTGTCGACAGCCACTCGTTTTACGTCATTGGTTATTTTGAAGAAACCAAGTTGCGCCATATCCGCGAAGGTGCACCAGCACAAATTACCCTCTATAGTGATAACAAAACGTTACAGGGTCACGTTTCCAGTATCGGTCGGGCGATTTATGATCAGAGCGTTGAAAGTGACTCAAGCCTTATTCCGGATGTTAAACCTAACGTCCCCTGGGTTCGACTCGCCCAACGCGTTCCCGTC  >  NC_000913/1721774‑1722043

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑