Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,525,760	0	G	A	20.3%	146.4 / 10.3	59	S74L (TCG→TTG)	ansP	L‑asparagine transporter
Reads supporting (aligned to +/- strand):  ref base G (33/14);  new base A (0/12);  total (33/26)
Fisher's exact test for biased strand distribution p-value = 8.63e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

GTTGATGAAGTACATCCAGCCAGCAACATAAGCGGCTTTCTCACCCAAAAA‑CTCACGGGCATAAGAAACAAAA‑CTGCCACTGGAAGGGCGGTGTAGCACCAGCTCACCCAATGCACGCAGAATAAAAAACGAAAACAAGCCACAAATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTGCACCTGGCGATTGCCCATCGCTTT  >  NC_000913/1525630‑1525899                                                                                                                                     |                                                                                                                                            gTTGATGAAGTACATCCAGCCAGCAACATAAGCGGCTTTCTCACCCAAAAA‑CTCACGGGCATAAGAAACAAAA‑CTGCCACTGGAAGGGCGGTGTAGCACCAGCTCACCCAATGCACGCAGAATAAAAAACGAAAACa                                                                                                                                       >  2:49346/1‑137 (MQ=255) gTTGATGAAGTACATCCAGCCAGCAACATAAGCGGCTTTCTCACCCAAAAA‑CTCACGGGCATAAGAAACAAAA‑CTGCCACTGGAAGGGCGGTGTAGCACCAGCTCACCCAATGCACGCAGAATAAAAAACGAAAACa                                                                                                                                       <  1:49346/137‑1 (MQ=255)       gaAGTACATCCAGCCAGCAACATAAGCGGCTTTCTCCCCCAAAAC‑CTCACGGGCATAAGAAAAAAAA‑CTGCCACTGGAAGGGCGGTGTAGCACCAGCTCACCCAATGCACGCAGAATAAAAAACGAAAACAAGCCACaaa                                                                                                                              <  2:1188124/140‑1 (MQ=255)       gaAGTACACCCAGCCAGCAACATAAGCGGCTTTCTCCCCAAAAAA‑CTCACGGGAAAAAGAAAAAAAA‑CTGCCACTGGAAGGGCGGTGTAGCACCAGCTCACCCAATGCACGCAGAATAAAAAACGAAAACAAGCCACaaa                                                                                                                              <  2:65895/140‑1 (MQ=255)        aaGTACATCCAGCCAGCAACATAAGCGGCTTTCTCACCCAAAAA‑CTCACGGGCATAAGAAACAAAA‑CTGCCACTGGAAGGGCGGTGTAGCACCAGCTCACCCAATGCACGCAGAATAAAAAACGAAAACAAGCCACAAAt                                                                                                                             >  1:805953/1‑140 (MQ=255)            ccATCCAGCCAGCAACATAAGCGGCTTTCTCCCCAAAAAC‑CTCACGGGCATAAGAAAAAAAA‑CTGCCACTGGAAGGGCGGTGTAGCACCAGCTCACCCAATGCACGCAGAATAAAAAACGAAAACAAGCCACAAATtaaa                                                                                                                         <  2:451554/139‑1 (MQ=255)            aCATCCAGCCAGCAACATAAGCGGCTTTCTCACCCAAAAA‑CTCACGGGCATAAGAAACAAAA‑CTGCCACTGGAAGGGCGGTGTAGCACCAGCTCACCCAATGCACGCAGAATAAAAAACGAAAACAAGCCACAAATtaaa                                                                                                                         >  2:1001917/1‑140 (MQ=255)            aCATCCAGCCAGCAACATAAGCGGCTTTCTCACCCAAAAA‑CTCACGGGCATAAGAAACAAAA‑CTGCCACTGGAAGGGCGGTGTAGCACCAGCTCACCCAATGCACGCAGAATAAAAAACGAAAACAAGCCACAAATtaaa                                                                                                                         >  1:863192/1‑140 (MQ=255)            aCATCCAGCCAGCAACATAAGCGGCTTTCTCACCCAAAAA‑CTCACGGGCATAAGAAACAAAA‑CTGCCACTGGAAGGGCGGTGTAGCACCAGCTCACCCAATGCACGCAGAATAAAAAACGAAAACAAGCCACAAATtaaa                                                                                                                         >  2:439336/1‑140 (MQ=255)             cATCCAGCCAGCAACATAAGCGGCTTTCTCACCCAAAAA‑CTCACGGGCATAAGAAACAAAA‑CTGCCACTGGAAGGGCGGTGTAGCACCAGCTCACCCAATGCACGCAGAATAAAAAACGAAAACAAGCCACAAATtaaat                                                                                                                        >  1:1128785/1‑140 (MQ=255)               tCCAGCCAGCAACATAAGCGGCTTTCTCACCCAAAAA‑CTCACGGGCATAAGAAACAAAA‑CTGCCACTGGAAGGGCGGTGTAGCACCAGCTCACCCAATGCACGCAGAATAAAAAACGAAAACAAGCCACAAATtaaataa                                                                                                                      >  1:65895/1‑140 (MQ=255)               tCCAGCCAGCAACATAAGCGGCTTTCTCACCCAAAAA‑CTCACGGGCATAAGAAACAAAA‑CTGCCACTGGAAGGGCGGTGTAGCACCAGCTCACCCAATGCACGCAGAATAAAAAACGAAAACAAGCCACAAATtaaataa                                                                                                                      >  2:37957/1‑140 (MQ=255)                ccagccagCAACATAAGCGGCTTTCTCACCCAAAAA‑CTCACGGGCATAAGAAACAAAA‑CTGCCACTGGAAGGGCGGTGTAGCACCAGCTCACCCAATGCACGCAGAATAAAAAACGAAAACAAGCCACAAATtaaataaa                                                                                                                     >  1:72297/1‑140 (MQ=255)                  agccagcAACATAAGCGGCTTTCTCACCCAAAAA‑CTCACGGGCATAAGAAACAAAA‑CTGCCACTGGAAGGGCGGTGTAGCACCAGCTCACCCAATGCACGCAGAATAAAAAACGAAAACAAGCCACAAATTAAATAAAcc                                                                                                                   <  1:773537/140‑1 (MQ=255)                    ccagcAACATAAGCGGCTTTCTCACCCAAAAA‑CTCACGGGCATAAGAAACAAAA‑CTGCCACTGGAAGGGCGGTGTAGCACCAGCTCACCCAATGCACGCAGAATAAAAAACGAAAACAAGCCACAAATTAAATAAAccag                                                                                                                 >  1:804290/1‑140 (MQ=255)                    ccagcAACATAAGCGGCTTTCTCACCCAAAAA‑CTCACGGGCATAAGAAACAAAA‑CTGCCACTGGAAGGGCGGTGTAGCACCAGCTCACCCAATGCACGCAGAATAAAAAACGAAAACAAGCCACAAATTAAATAAAccag                                                                                                                 >  1:700449/1‑140 (MQ=255)                       gcAACATAAGCGGCTTTCTCCCCAAAAAA‑CCCACGGGCAAAAAAAAAAAAA‑CTGCCACTGGAAGGGCGGTGTAGCACCAGCTCACCCAATGCACGCAGAATAAAAAACGAAAACAAGCCACAAATTAAATAAAccagtgc                                                                                                              <  2:805953/140‑1 (MQ=255)                       gcAACATAAGCGGCTTTCTCACCCAAAAA‑CTCACGGGCATAAGAAACAAAA‑CTGCCACTGGAAGGGCGGTGTAGCACCAGCTCACCCAATGCACGCAGAATAAAAAACGAAAACAAGCCACAAATTAAATAAAccagtgc                                                                                                              <  1:1046814/140‑1 (MQ=255)                       gcAACATAAGCGCCTTTCTCCCCAAAAAA‑CTCCCGGGCATAAAAAACAAAA‑CTGCCACTGGAAGGGCGGTGTAGCACCAGCTCACCCAATGCACGCAGAATAAAAAACGAAAACAAGCCACAAATTAAATAAAccagtgc                                                                                                              <  2:804290/140‑1 (MQ=255)                       gcAAAATAAGCGGTTTTCTCACCCAAAAA‑CTCAGGGCCATAAAAAACAAAA‑CTGCCACGGGAAGGGCGGTGTAGCCCCAGCTCACCCAATGCACGCAGAATAAAAACCGAAAACAAGCCACAAATTAAATAAAccagtgc                                                                                                              <  2:116962/140‑1 (MQ=255)                                gCGGCTTTCTCACCCAAAAA‑CTCACGGGCATAAGAAACAAAA‑CTGCCACTGGAAGGGCGGTGTAGCACCAGCTCACCCAATGCACGCAGAATAAAAAACGAAAACAAGCCACAAATTAAATAAACCAGTGCCAGTGCggg                                                                                                     >  1:428223/1‑140 (MQ=255)                                  ggCTTTCCCCCCAAAAAC‑CCCACGGGAAAAAAAAAAAAAC‑CTGCCACGGGAAGGGGGGTGTACCACCAGCCCACCCAAGGCACGCAAAAAAAAAAACAAAAACAAGCCACAAATTAAATAAACCAGTGCCAGTGCGGGcc                                                                                                   <  2:1019025/140‑1 (MQ=255)                                      ttCTCACCCAAAAA‑CTCACGGGCATAAGAAACAAAA‑CTGCCACTGGAAGGGCGGTGTAGCACCAGCTCACCCAATGCACGCAGAATAAAAAACGAAAACAAGCCACAAATTAAATAAACCAGTGCCAGTGCGGGCCCCGc                                                                                               >  1:758623/1‑140 (MQ=255)                                           ccccAAAAACCTCAGGGGAAAAAGAAAAAAAA‑CTGCCACTGGAAGGGCGGTGTAGCACCAGCTCACCAAATGCCCGCAGAATAAAAAACGAAAACAAGCCACAAATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCAtt                                                                                           <  1:705893/139‑1 (MQ=255)                                               aaaaa‑CCCAGGGCAAAAAAAAAAAAAA‑CTGCCACTGGAAGGGCGGTGTAGCACCAGCCCACCCAATGCACGCAGAATAAAAAACGAAAACAAGCCACAAATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAg                                                                                      <  2:1111932/140‑1 (MQ=255)                                                  aa‑CTCACGGGCATAAGAAACAAAA‑CTGCCACTGGAAGGGCGGTGTAGCACCAGCTCACCCAATGCACGCAGAATAAAAAACGAAAACAAGCCACAAATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCg                                                                                   >  2:313752/1‑140 (MQ=255)                                                   acctccggggAATAAAAAAAAAAC‑CTGCCACTGGAAGGGCGGTGTAGCACCAGCTCCCCAAATGCACGCAGAAAAAAAAACAAAAACAAGCCACAAATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCg                                                                                   <  2:1148722/133‑1 (MQ=255)                                                    acccccGGGCAAAAAAAAAAAAACCTGCCACGGGAGGGGGGGTGAAGCACCAGCCCCCCCAATGCACGCAAAATAAAAAACAAAAACAAGCCACAAATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCg                                                                                   <  2:431300/135‑1 (MQ=255)                                                        aCGGGCATAAGAAACAAAA‑CTGCCACTGGAAGGGCGGTGTAGCACCAGCTCACCCAATGCACGCAGAATAAAAAACGAAAACAAGCCACAAATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTc                                                                              >  2:1227513/1‑140 (MQ=255)                                                          gggCATAAGAAACAAAA‑CTGCCACTGGAAGGGCGGTGTAGCACCAGCTCACCCAATGCACGCAGAATAAAAAACGAAAACAAGCCACAAATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCt                                                                            >  2:1166014/1‑140 (MQ=255)                                                           gcaaTAAAAAAAAAAA‑CGGCCACTGAAAGGGCGGTGTAGCACCAGCTCACCCAAGACGCGCAGAATAAAAAACAAAAACAAGCCACAAATTAAATAACCCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTg                                                                           <  2:795383/137‑1 (MQ=255)                                                                      cAAAA‑CTGCCACTGGAAGGGCGGTGTAGCACCAGCTCACCCAATGCACGCAGAATAAAAAACGAAAACAAGCCACAAATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAAc                                                                >  1:1075849/1‑140 (MQ=255)                                                                       aaaa‑CTGCCACTGGAAGGGCGGTGTAGCACCAGCTCACCCAATGCACGCAGAATAAAAAACGAAAACAAGCCACAAATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACa                                                               >  2:842662/1‑140 (MQ=255)                                                                         aa‑CTGCCACTGGAAGGGCGGTGTAGCACCAGCTCACCAAATGCACGCAGAAAAAAAAACAAAAACAAGCCACAAATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAg                                                             <  1:439336/140‑1 (MQ=255)                                                                           accgCCACTGGAAGGGGGGTGTAGCCCCAGCCCCCCAAATGCACGAAAAAAAAAAAACAAAAACAAGCCACAAATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGc                                                            <  2:372698/137‑1 (MQ=255)                                                                              gCCACTGGAAGGGCGGTGTAGCACCAGCTCACCCAATGCACGCAGAATAAAAAACGAAAACAAGCCACAAATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCgg                                                         >  1:1151115/1‑140 (MQ=255)                                                                                        ggggggTGAACCACCACCCCCCCAAATCCACGAAAAAAAAAAACCAAAAACAAGCCACAAATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATcgc                                               <  2:3360/140‑1 (MQ=255)                                                                                         ggCGGTGAACCACCACCCCCCCCAATCCACCAAAAAAAAAAAACAAAAACAAGCCACAAATTAAAAAACCCAGTCCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATcgcg                                              <  2:428223/140‑1 (MQ=255)                                                                                             gtgtAGCACCAGCTCACCCAATGCACGCAGAATAAAAAACGAAAACAAGCCACAAATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGcgccgc                                          >  1:1235163/1‑140 (MQ=255)                                                                                               gtAGCACCAGCTCACCCAATGCACGCAGAATAAAAAACGAAAACAAGCCACAAATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCa                                        >  2:372560/1‑140 (MQ=255)                                                                                                tAGCACCAGCTCACCCAATGCACGCAGAATAAAAAACGAAAACAAGCCACAAATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCaa                                       >  2:827527/1‑140 (MQ=255)                                                                                                     ccAGCTCACCCAATGCACGCAGAATAAAAAACGAAAACAAGCCACAAATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCg                                  >  1:602664/1‑140 (MQ=255)                                                                                                        gCTCACCCAATGCACGCAGAATAAAAAACGAAAACAAGCCACAAATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGatc                               >  2:647090/1‑140 (MQ=255)                                                                                                        gCTCACCCAATGCACGCAGAATAAAAAACGAAAACAAGCCACAAATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGatc                               >  2:68319/1‑140 (MQ=255)                                                                                                        gCTCACCCAATGCACGCAGAATAAAAAACGAAAACAAGCCACAAATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGatc                               >  2:846189/1‑140 (MQ=255)                                                                                                        gCTCACCCAATGCACGCAGAATAAAAAACGAAAACAAGCCACAAATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGatc                               >  1:10295/1‑140 (MQ=255)                                                                                                          cccccaaaTGCAGGCAAAAAAAAAAACAAAAAAAAGCCACAAATAAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGatcat                             <  2:602664/134‑1 (MQ=255)                                                                                                            cccaacggCACGGAAAAAAAAAAACGAAAACAAGCCACAAATTAAATAAACCGGGGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCt                           <  1:397225/133‑1 (MQ=255)                                                                                                             cccAATGCACGCAGAATAAAAAACGAAAACAAGCCACAAATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTg                          >  2:1249027/1‑140 (MQ=255)                                                                                                             cccAATGCACGCAGAATAAAAAACGAAAACAAGCCACAAATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTg                          >  2:704566/1‑140 (MQ=255)                                                                                                             ccaaaTGCCCGCAAAAAAAAAAACAAAAACAAGCCACAAATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTg                          <  1:177449/137‑1 (MQ=255)                                                                                                                 aTGCACGCAAAAAAAAAAACAAAAACAAGCCACAAATAAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTGCAcc                      <  2:1151115/140‑1 (MQ=255)                                                                                                                  tGCACGCAGAATAAAAAACGAAAACAAGCCACAAATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTGCACCt                     >  1:766282/1‑140 (MQ=255)                                                                                                                       gCAGAAAAAAAAACGAAAACAAGCCACAAATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTGCACCTGGCGa                <  1:1239209/140‑1 (MQ=255)                                                                                                                        cAGAATAAAAAACGAAAACAAGCCACAAATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTGCACCTGGCGAt               >  2:387078/1‑140 (MQ=255)                                                                                                                        cAGAATAAAAAACGAAAACAAGCCACAAATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTGCACCTGGCGAt               >  1:619416/1‑140 (MQ=255)                                                                                                                             tAAAAAACGAAAACAAGCCACAAATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTGCACCTGGCGATTGccc          >  2:388574/1‑140 (MQ=255)                                                                                                                              aaaaaaCAAAAACAAGCCACAAATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTGCACCTGGCGATTGCCCa         <  2:1075849/140‑1 (MQ=255)                                                                                                                              aaaaaaCAAAAAAAAGCCACAAATTAAATAACCCAGTGCCAGGGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTGCACCTGGCGATTGCCCa         <  2:217532/140‑1 (MQ=255)                                                                                                                               aaaaacaaaaacAAGCCACAAATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTGCACCTGGCGATTGCCCAt        <  1:1001917/140‑1 (MQ=255)                                                                                                                                 aaaCGAAAACAAGCCACAAATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTGCACCTGGCGATTGCCCATCg      <  1:68319/140‑1 (MQ=255)                                                                                                                                     gAAAACAAGCCACAAATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTGCACCTGGCGATTGCCCATCGCttt  >  1:332268/1‑140 (MQ=255)                                                                                                                                     gAAAACAAGCCACAAATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTGCACCTGGCGATTGCCCATCGCttt  >  2:230593/1‑140 (MQ=255)                                                                                                                                     |                                                                                                                                            GTTGATGAAGTACATCCAGCCAGCAACATAAGCGGCTTTCTCACCCAAAAA‑CTCACGGGCATAAGAAACAAAA‑CTGCCACTGGAAGGGCGGTGTAGCACCAGCTCACCCAATGCACGCAGAATAAAAAACGAAAACAAGCCACAAATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTGCACCTGGCGATTGCCCATCGCTTT  >  NC_000913/1525630‑1525899

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑