Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,802,435	C→A	D46Y (GAT→TAT)	thrS ←	threonine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,802,435	0	C	A	100.0%	163.6 / NA	47	D46Y (GAT→TAT)	thrS	threonine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (21/26);  total (21/26)

CGGCCAAAGTTGTTTAATCGCGTGCCCTAACAGGTGCGCACAGGAGTGACGAATGATCTCCAGACCTTCTTCGTCTTTGGCGGTAATGATCGACAGTTGTGCGTCGTTTTCAATCAGATCGCAAGCATCAACCAGTTCGCCATTAACGCGCCCTGCGATACAGGCTTTCGCCAGACCTGGACCAATGTCCAGCGCAACATCCATGGGGCTTACAGCGTGATCGTAATGGCGTTGGCTGCCATCAGGAAGAGTTATAACAGGC  >  NC_000913/1802307‑1802568                                                                                                                                 |                                                                                                                                      cGGCCAAAGTTGTTTAATCGCGTGCCCTAACAGGTGCGCACAGGAGTGACGAATGATCTCCAGACCTTCTTCGTCTTTGGCGGTAATGATCGACAGTTGTGCGTCGTTTTCAATCAGATCGCAAGCATAAACCAGTTCGc                                                                                                                            >  2:1145470/1‑140 (MQ=255)           tgttTAATCGCGTGCCCTAACAGGTGCGCACAGGAGTGACGAATGATCTCCAGACCTTCTTCGTCTTTGGCGGTAATGATCGACAGTTGTGCGTCGTTTTCAATCAGATCGCAAGCATAAACCAGTTCGCCATTAAcgcg                                                                                                                  <  2:92999/140‑1 (MQ=255)           tgttTAATCGCGTGCCCTAACAGGTGCGCACAGGAGTGACGAATGATCTCCAGACCTTCTTCGTCTTTGGCGGTAATGATCGACAGTTGTGCGTCGTTTTCAATCAGATCGCAAGCATAAACCAGTTCGCCATTAAcgcg                                                                                                                  <  2:475290/140‑1 (MQ=255)           tgttTAATCGCGTGCCCTAACAGGTGCGCACAGGAGTGACGAATGATCTCCAGACCTTCTTCGTCTTTGGCGGTAATGATCGACAGTTGTGCGTCGTTTTCAATCAGATCGCAAGCATAAACCAGTTCGCCATTAAcgcg                                                                                                                  <  1:14145/140‑1 (MQ=255)              ttAATCGCGTGCCCTAACAGGTGCGCACAGGAGTGACGAATGATCTCCAGACCTTCTTCGTCTTTGGCGGTAATGATCGACAGTTGTGCGTCGTTTTCAATCAGATCGCAAGCATAAACCAGTTCGCCATTAACGCGccc                                                                                                               >  1:70696/1‑140 (MQ=255)                                aGGTGCGCACAGGAGTGACGAATGATCTCCAGACCTTCTTCGTCTTTGGCGGTAATGATCGACAGTTGTGCGTCGTTTTCAATCAGATCGCAAGCATAAACCAGTTCGCCATTAACGCGCCCTGCGATACAGGCTTTCGc                                                                                             <  1:382092/140‑1 (MQ=255)                                    gcgcACAGGAGTGACGAATGATCTCCAGACCTTCTTCGTCTTTGGCGGTAATGATCGACAGTTGTGCGTCGTTTTCAATCAGATCGCAAGCATAAACCAGTTCGCCATTAACGCGCCCTGCGATACAGGCTTTCGcc                                                                                            >  1:990414/1‑137 (MQ=255)                                    gcgcACAGGAGTGACGAATGATCTCCAGACCTTCTTCGTCTTTGGCGGTAATGATCGACAGTTGTGCGTCGTTTTCAATCAGATCGCAAGCATAAACCAGTTCGCCATTAACGCGCCCTGCGATACAGGCTTTCGcc                                                                                            <  2:990414/137‑1 (MQ=255)                                     cgcACAGGAGTGACGAATGATCTCCAGACCTTCTTCGTCTTTGGCGGTAATGATCGACAGTTGTGCGTCGTTTTCAATCAGATCGCAAGCATAAACCAGTTCGCCATTAACGCGCCCTGCGATACAGGCTTTCGCCAGAc                                                                                        <  1:423527/140‑1 (MQ=255)                                     cgcACAGGAGTGACGAATGATCTCCAGACCTTCTTCGTCTTTGGCGGTAATGATCGACAGTTGTGCGTCGTTTTCAATCAGATCGCAAGCATAAACCAGTTCGCCATTAACGCGCCCTGCGATACAGGCTTTCGCCAGAc                                                                                        >  1:592877/1‑140 (MQ=255)                                       cacaGGAGTGACGAATGATCTCCAGACCTTCTTCGTCTTTGGCGGTAATGATCGACAGTTGTGCGTCGTTTTCAATCAGATCGCAAGCATAAACCAGTTCGCCATTAACGCGCCCTGCGATACAGGCTTTCGCCAGACCt                                                                                      >  2:154714/1‑140 (MQ=255)                                       cacaGGAGTGACGAATGATCTCCAGACCTTCTTCGTCTTTGGCGGTAATGATCGACAGTTGTGCGTCGTTTTCAATCAGATCGCAAGCATAAACCAGTTCGCCATTAACGCGCCCTGCGATACAGGCTTTCGCCAGACCt                                                                                      >  2:709498/1‑140 (MQ=255)                                                               gACCTTCTTCGTCTTTGGCGGTAATGATCGACAGTTGTGCGTCGTTTTCAATCAGATCGCAAGCATAAACCAGTTCGCCATTAACGCGCCCTGCGATACAGGCTTTCGCCAGACCTGGACCAATGTCCAGCGCAACATcc                                                              <  2:404470/140‑1 (MQ=255)                                                                 ccTTCTTCGTCTTTGGCGGTAATGATCGACAGTTGTGCGTCGTTTTCAATCAGATCGCAAGCATAAACCAGTTCGCCATTAACGCGCCCTGCGATACAGGCTTTCGCCAGACCTGGACCAATGTCCAGCGCAACATCCAt                                                            <  1:1019831/140‑1 (MQ=255)                                                                   ttcttcGTCTTTGGCGGTAATGATCGACAGTTGTGCGTCGTTTTCAATCAGATCGCAAGCATAAACCAGTTCGCCATTAACGCGCCCTGCGATACAGGCTTTCGCCAGACCTGGACCAATGTCCAGCGCaa                                                                   <  2:716231/131‑1 (MQ=255)                                                                   ttcttcGTCTTTGGCGGTAATGATCGACAGTTGTGCGTCGTTTTCAATCAGATCGCAAGCATAAACCAGTTCGCCATTAACGCGCCCTGCGATACAGGCTTTCGCCAGACCTGGACCAATGTCCAGCGCaa                                                                   >  1:716231/1‑131 (MQ=255)                                                                   ttcttcGTCTTTGGCGGTAATGATCGACAGTTGTGCGTCGTTTTCAATCAGATCGCAAGCATAAACCAGTTCGCCATTAACGCGCCCTGCGATACAGGCTTTCGCCAGACCTGGACCAATGTCCAGCGCAACATCCATgg                                                          <  1:1276744/140‑1 (MQ=255)                                                                   ttcttcGTCTTTGGCGGTAATGATCGACAGTTGTGCGTCGTTTTCAATCAGATCGCAAGCATAAACCAGTTCGCCATTAACGCGCCCTGCGATACAGGCTTTCGCCAGACCTGGACCAATGTCCAGCGCAACATCCATgg                                                          <  1:1057038/140‑1 (MQ=255)                                                                     cttcGTCTTTGGCGGTAATGATCGACAGTTGTGCGTCGTTTTCAATCAGATCGCAAGCATAAACCAGTTCGCCATTAACGCGCCCTGCGATACAGGCTTTCGCCATACCTGGACCAATGTCCAGCGCAACATCCATgggg                                                        >  2:1266864/1‑140 (MQ=255)                                                                     cttcGTCTTTGGCGGTAATGATCGACAGTTGTGCGTCGTTTTCAATCAGATCGCAAGCATAAACCAGTTCGCCATTAACGCGCCCTGCGATACAGGCTTTCGCCAGACCTGGACCAATGTCCAgcgc                                                                     <  2:1261861/127‑1 (MQ=255)                                                                     cttcGTCTTTGGCGGTAATGATCGACAGTTGTGCGTCGTTTTCAATCAGATCGCAAGCATAAACCAGTTCGCCATTAACGCGCCCTGCGATACAGGCTTTCGCCAGACCTGGACCAATGTCCAgcgc                                                                     >  2:91584/1‑127 (MQ=255)                                                                     cttcGTCTTTGGCGGTAATGATCGACAGTTGTGCGTCGTTTTCAATCAGATCGCAAGCATAAACCAGTTCGCCATTAACGCGCCCTGCGATACAGGCTTTCGCCAGACCTGGACCAATGTCCAgcgc                                                                     <  1:91584/127‑1 (MQ=255)                                                                     cttcGTCTTTGGCGGTAATGATCGACAGTTGTGCGTCGTTTTCAATCAGATCGCAAGCATAAACCAGTTCGCCATTAACGCGCCCTGCGATACAGGCTTTCGCCAGACCTGGACCAATGTCCAgcgc                                                                     >  1:1261861/1‑127 (MQ=255)                                                                         gTCTTTGGCGGTAATGATCGACAGTTGTGCGTCGTTTTCAATCAGATCGCAAGCATAAACCAGTTCGCCATTAACGCGCCCTGCGATACAGGCTTTCGCCAGACCTGGACCAATGTCCAGCGCAACATCCATGGGGCTTa                                                    <  2:592877/140‑1 (MQ=255)                                                                             ttGGCGGTAATGATCGACAGTTGTGCGTCGTTTTCAATCAGATCGCAAGCATAAACCAGTTCGCCATTAACGCGCCCTGCGATACAGGCTTTCGCCAGACCTGGACCAATGTCCAGCGCAACATCCATGGGGCTTACAGc                                                <  1:1266864/140‑1 (MQ=255)                                                                              tGGCGGTAATGATCGACCGATGTGCGTCGCTTTCAATCAGATCGCAAGCATAAACCAGTTCGCCCTTAACGCGCCCTGCGATACAGGCTTTCGCCAGACCTGGACCAATGTCCAGCGCAACATCCTTGGGGCTTACAGCg                                               >  2:580310/1‑140 (MQ=255)                                                                                    tAATGATCGACAGTTGTGCGTCGTTTTCAATCAGATCGCAAGCATAAACCAGTTCGCCATTAACGCGCCCTGCGATACAGGCTTTCGCCAGACCTGGACCAATGTCCAGCGCAACATCCATGGGGCTTACAGCGTGATCg                                         >  1:1282301/1‑140 (MQ=255)                                                                                         aTCGACAGTTGTGCGTCGTTTTCAATCAGATCGCAAGCATAAACCAGTTCGCCATTAACGCGCCCTGCGATACAGGCTTTCGCCAGACCTGGACCAATGTCCAGCGCAACATCCATGGGGCTTACAGCGTGATCGTAATg                                    >  2:486077/1‑140 (MQ=255)                                                                                           cGACAGTTGTGCGTCGTTTTCAATCAGATCGCAAGCATAAACCAGTTCGCCATTAACGCGCCCTGCGATACAGGCTTTCGCCAGACCTGGACCAATGTCCAGCGCAACATCCATGGGGCTTACAGCGTGATCGTAATGGc                                  <  1:315596/140‑1 (MQ=255)                                                                                            gACAGTTGTGCGTCGTTTTCAATCAGATCGCAAGCATAAACCAGTTCGCCATTAACGCGCCCTGCGATACAGGCTTTCGCCAGACCTGGACCAATGTCCAGCGCAACATCCATGGGGCTTACAGCGTGATCGTAATGGCg                                 >  1:931608/1‑140 (MQ=255)                                                                                                 ttGTGCGTCGTTTTCAATCAGATCGCAAGCATAAACCAGTTCGCCATTAACGCGCCCTGCGATACAGGCTTTCGCCAGACCTGGACCAATGTCCAGCGCAACATCCATGGGGCTTACAGCGTGATCGTAATGGCGTTGGc                            >  1:1224100/1‑140 (MQ=255)                                                                                                 ttGTGCGTCGTTTTCAATCAGATCGCAAGCATAAACCAGTTCGCCATTAACGCGCCCTGCGATACAGGCTTTCGCCAGACCTGGACCAATGTCCAGCGCAACATCCATGGGGCTTACAGCGTGATCGTAATGGCGTTGGc                            <  1:709498/140‑1 (MQ=255)                                                                                                   gtgCGTCGTTTTCAATCAGATCGCAAGCATAAACCAGTTCGCCATTAACGCGCCCTGCGATACAGGCTTTCGCCAGACCTGGACCAATGTCCAGCGCAACATCCATGGGGCTTACAGCGTGATCGTAATGGCGTTGGCTg                          >  2:284285/1‑140 (MQ=255)                                                                                                   gtgCGTCGTTTTCAATCAGATCGCAAGCATAAACCAGTTCGCCATTAACGCGCCCTGCGATACAGGCTTTCGCCAGACCTGGACCAATGTCCAGCGCAACATCCATGGGGCTTACAGCGTGATCGTAATGGCGTTGGCTg                          >  2:463357/1‑140 (MQ=255)                                                                                                      cgtcgtTTTCAATCAGATCGCAAGCATAAACCAGTTCGCCATTAACGCGCCCTGCGATACAGGCTTTCGCCAGACCTGGACCAATGTCCAGCGCAACATCCATGGGGCTTACAGCGTGATCGTAATGGCGTTGGCTGCCa                       >  1:1031743/1‑140 (MQ=255)                                                                                                         cgtTTTCAATCAGATCGCAAGCATAAACCAGTTCGCCATTAACGCGCCCTGCGATACAGGCTTTCGCCAGACCTGGACCAATGTCCAGCGCAACATCCATGGGGCTTACAGCGTGATCGTAATGGCGTTGGCTGCCATCa                    >  2:887661/1‑140 (MQ=255)                                                                                                           ttttCAATCAGATCGCAAGCATAAACCAGTTCGCCATTAACGCGCCCTGCGATACAGGCTTTCGCCAGACCTGGACCAATGTCCAGCGCAACATCCATGGGGCTTACAGCGTGATCGTAATGGCGTTGGCTGCCa                       <  1:682088/135‑1 (MQ=255)                                                                                                           ttttCAATCAGATCGCAAGCATAAACCAGTTCGCCATTAACGCGCCCTGCGATACAGGCTTTCGCCAGACCTGGACCAATGTCCAGCGCAACATCCATGGGGCTTACAGCGTGATCGTAATGGCGTTGGCTGCCa                       >  2:682088/1‑135 (MQ=255)                                                                                                            tttCAATCAGATCGCAAGCATAAACCAGTTCGCCATTAACGCGCCCTGCGATACAGGCTTTCGCCAGACCTGGACCAATGTCCAGCGCAACATCCATGGGGCTTACAGCGTGATCGTAATGGCGTTGGCTGCCATCAGGa                 <  1:463357/140‑1 (MQ=255)                                                                                                            tttCAATCAGATCGCAAGCATAAACCAGTTCGCCATTAACGCGCCCTGCGATACAGGCTTTCGCCAGACCTGGACCAATGTCCAGCGCAACATCCATGGGGCTTACAGCGTGATCGTAATGGCGTTGGCTGCCATCAGGa                 <  1:918213/140‑1 (MQ=255)                                                                                                             ttCAATCAGATCGCAAGCATAAACCAGTTCGCCATTAACGCGCCCTGCGATACAGGCTTTCGCCAGACCTGGACCAATGTCCAGCGCAACATCCATGGGGCTTACAGCGTGATCGTAATGGCGTTGGCTGCCATCAGGaa                <  2:1031743/140‑1 (MQ=255)                                                                                                                    aGATCGCAAGCATAAACCAGTTCGCCATTAACGCGCCCTGCGATACAGGCTTTCGCCAGACCTGGACCAATGTCCAGCGCAACATCCATGGGGCTTACAGCGTGATCGTAATGGCGTTGGCTGCCATCAGGAAGAGTtat         <  2:992456/140‑1 (MQ=255)                                                                                                                     gATCGCAAGCATAAACCAGTTCGCCATTAACGCGCCCTGCGATACAGGCTTTCGCCAGACCTGGACCAATGTCCAGCGCAACATCCATGGGGCTTACAGCGTGATCGTAATGGCGTTGGCTGCCAt                      <  2:1188752/126‑1 (MQ=255)                                                                                                                     gATCGCAAGCATAAACCAGTTCGCCATTAACGCGCCCTGCGATACAGGCTTTCGCCAGACCTGGACCAATGTCCAGCGCAACATCCATGGGGCTTACAGCGTGATCGTAATGGCGTTGGCTGCCAt                      >  1:1188752/1‑126 (MQ=255)                                                                                                                      aTCGCAAGCATAAACCAGTTCGCCATTAACGCGCCCTGCGATACAGGCTTTCGCCAGACCTGGACCAATGTCCAGCGCAACATCCATGGGGCTTACAGCGTGATCGTAATGGCGTTGGCTGCCATCAGGAAGAGTTATaa       >  2:856009/1‑140 (MQ=255)                                                                                                                        cGCAAGCATAAACCAGTTCGCCATTAACGCGCCCTGCGATACAGGCTTTCGCCAGACCTGGACCAATGTCCAGCGCAACATCCATGGGGCTTACAGCGTGATCGTAATGGCGTTGGCTGCCATCAGGAAGAGTTATAACa     >  2:856951/1‑140 (MQ=255)                                                                                                                          cAAGCATAAACCAGTTCGCCATTAACGCGCCCTGCGATACAGGCTTTCGCCAGACCTGGACCAATGTCCAGCGCAACATCCATGGGGCTTACAGCGTGATCGTAATGGCGTTGGCTGCCATCAGGAAGAGTTATAACAgg   <  2:1224100/140‑1 (MQ=255)                                                                                                                          cAAGCATAAACCAGTTCGCCATTAACGCGCCCTGCGATACAGGCTTTCGCCAGACCTGGACCAATGTCCAGCGCAACATCCATGGGGCTTACAGCGTGATCGTAATGGCGTTGGCTGCCATCAGGAAGAGTTATAACAgg   <  1:580310/140‑1 (MQ=255)                                                                                                                           aaGCATAAACCAGTTCGCCATTAACGCGCCCTGCGATACAGGCTTTCGCCAGACCTGGACCAATGTCCAGCGCAACATCCATGGGGCTTACAGCGTGATCGTAATGGCGTTGGCTGCCATCAGGAAGAGTTATAACAGGc  <  2:931608/140‑1 (MQ=255)                                                                                                                                 |                                                                                                                                      CGGCCAAAGTTGTTTAATCGCGTGCCCTAACAGGTGCGCACAGGAGTGACGAATGATCTCCAGACCTTCTTCGTCTTTGGCGGTAATGATCGACAGTTGTGCGTCGTTTTCAATCAGATCGCAAGCATCAACCAGTTCGCCATTAACGCGCCCTGCGATACAGGCTTTCGCCAGACCTGGACCAATGTCCAGCGCAACATCCATGGGGCTTACAGCGTGATCGTAATGGCGTTGGCTGCCATCAGGAAGAGTTATAACAGGC  >  NC_000913/1802307‑1802568

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑